AlfalfaGEDB | MS.gene90155

M. sativa cultivar XinJiangDaYe / MS.gene90155

KEGG: K24345
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13833.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90155.t1	XP_003596520.1	95.6	114	5	0	1	114	1	114	1.20E-57	232.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90155.t1	Q9LSQ6	56.8	95	39	2	9	101	19	113	7.1e-24	111.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90155.t1	Q2HVR3	95.6	114	5	0	1	114	1	114	8.3e-58	232.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050100	MS.gene90155	0.838974	2.09E-57	-1.69E-46
MS.gene055359	MS.gene90155	0.833843	4.22E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90155.t1	MTR_2g081440	95.614	114	5	0	1	114	1	114	1.76e-75	219
MS.gene90155.t1	MTR_2g081350	88.596	114	13	0	1	114	1	114	3.34e-67	198
MS.gene90155.t1	MTR_2g081300	85.321	109	16	0	6	114	2	110	8.84e-65	191
MS.gene90155.t1	MTR_2g011400	63.830	94	33	1	9	101	14	107	8.05e-36	119
MS.gene90155.t1	MTR_2g081580	59.794	97	38	1	7	102	8	104	2.09e-34	115
MS.gene90155.t1	MTR_8g466250	61.458	96	36	1	9	103	11	106	2.31e-34	115
MS.gene90155.t1	MTR_2g089210	56.122	98	42	1	2	98	3	100	1.55e-32	110
MS.gene90155.t1	MTR_1g052885	59.341	91	36	1	9	98	6	96	8.44e-31	106
MS.gene90155.t1	MTR_8g027180	53.191	94	43	1	6	98	16	109	1.03e-27	98.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90155.t1	AT5G54490	56.842	95	39	2	9	101	19	113	4.53e-32	109
MS.gene90155.t1	AT4G27280	55.208	96	41	2	8	101	18	113	1.78e-29	103
MS.gene90155.t1	AT2G46600	48.936	94	47	1	6	98	19	112	6.42e-25	92.0

Find 28 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTACGGGTTTCATTTGCTTA+TGG	0.204248	2.3:+16897661	MS.gene90155:CDS
CCATCCATGGTTGCGAGTTT+AGG	0.278334	2.3:+16897611	MS.gene90155:CDS
TTAGGAGCAGAAGCATTTAT+AGG	0.308592	2.3:+16897629	MS.gene90155:CDS
CATGCTAATGGAAGGTGATT+TGG	0.335601	2.3:+16897772	MS.gene90155:CDS
AAAATATGCACAAATCTTCA+AGG	0.428589	2.3:+16897872	MS.gene90155:CDS
TAGGTTGAGTCCATGTTTAA+TGG	0.441618	2.3:+16897841	MS.gene90155:CDS
CCTAAACTCGCAACCATGGA+TGG	0.475656	2.3:-16897611	None:intergenic
CACAAGTTTCCACATGGAGT+TGG	0.491047	2.3:+16897559	None:intergenic
AAGATGAATTGTTTCATGCT+TGG	0.497368	2.3:+16897719	MS.gene90155:CDS
TGAGGACTACTTTCCATCCA+TGG	0.498192	2.3:+16897598	MS.gene90155:CDS
TGAGCTCGTGTACATGCTAA+TGG	0.499730	2.3:+16897760	MS.gene90155:CDS
ATGAGAATGCAAAACTCCAT+TGG	0.509723	2.3:-16897815	None:intergenic
GAATTGTTTCATGCTTGGTA+TGG	0.522428	2.3:+16897724	MS.gene90155:CDS
TTGAGTCCATGTTTAATGGA+TGG	0.531601	2.3:+16897845	MS.gene90155:CDS
TTTATAGGTGAACTTTGCTA+CGG	0.531654	2.3:+16897644	MS.gene90155:CDS
TTATAGGTGAACTTTGCTAC+GGG	0.538698	2.3:+16897645	MS.gene90155:CDS
TTTGCTTATGGATGTCAACA+AGG	0.557910	2.3:+16897673	MS.gene90155:CDS
TCATGCTTGGTATGGAGGTG+AGG	0.562351	2.3:+16897732	MS.gene90155:CDS
CATGCTTGGTATGGAGGTGA+GGG	0.564464	2.3:+16897733	MS.gene90155:CDS
AGATGGTGCTTTAAATCCAA+TGG	0.565219	2.3:+16897799	MS.gene90155:CDS
TTGTTTCATGCTTGGTATGG+AGG	0.565973	2.3:+16897727	MS.gene90155:CDS
TGCTCCTAAACTCGCAACCA+TGG	0.581169	2.3:-16897615	None:intergenic
GAGTGTCATCACATTAACAT+TGG	0.590328	2.3:-16897902	None:intergenic
TTAGGTCCATCCATTAAACA+TGG	0.608146	2.3:-16897851	None:intergenic
GAAGGTGATTTGGATGGAGA+TGG	0.627275	2.3:+16897782	MS.gene90155:CDS
TTGCTTATGGATGTCAACAA+GGG	0.661501	2.3:+16897674	MS.gene90155:CDS
CTAATGGAAGGTGATTTGGA+TGG	0.674005	2.3:+16897776	MS.gene90155:CDS
CTCGTGTACATGCTAATGGA+AGG	0.692064	2.3:+16897764	MS.gene90155:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAATATGCACAAATCTTCA+AGG	+	chr2.3:16897872-16897891	MS.gene90155:CDS	25.0%
!!	TGAAGATTTGTGCATATTTT+AGG	-	chr2.3:16897872-16897891	None:intergenic	25.0%
	AAGATGAATTGTTTCATGCT+TGG	+	chr2.3:16897719-16897738	MS.gene90155:CDS	30.0%
	TTTATAGGTGAACTTTGCTA+CGG	+	chr2.3:16897644-16897663	MS.gene90155:CDS	30.0%
!	AGTTTTGCATTCTCATGTTT+AGG	+	chr2.3:16897822-16897841	MS.gene90155:CDS	30.0%
!!	GGATTTTGATATGGATTTTG+AGG	+	chr2.3:16897580-16897599	MS.gene90155:CDS	30.0%
	ATGAGAATGCAAAACTCCAT+TGG	-	chr2.3:16897818-16897837	None:intergenic	35.0%
	GAATTGTTTCATGCTTGGTA+TGG	+	chr2.3:16897724-16897743	MS.gene90155:CDS	35.0%
	TTAGGTCCATCCATTAAACA+TGG	-	chr2.3:16897854-16897873	None:intergenic	35.0%
	TTATAGGTGAACTTTGCTAC+GGG	+	chr2.3:16897645-16897664	MS.gene90155:CDS	35.0%
	TTGAGTCCATGTTTAATGGA+TGG	+	chr2.3:16897845-16897864	MS.gene90155:CDS	35.0%
	TTGCTTATGGATGTCAACAA+GGG	+	chr2.3:16897674-16897693	MS.gene90155:CDS	35.0%
	TTTGCTTATGGATGTCAACA+AGG	+	chr2.3:16897673-16897692	MS.gene90155:CDS	35.0%
!	AGATGGTGCTTTAAATCCAA+TGG	+	chr2.3:16897799-16897818	MS.gene90155:CDS	35.0%
!	TAGGTTGAGTCCATGTTTAA+TGG	+	chr2.3:16897841-16897860	MS.gene90155:CDS	35.0%
!!	TTAGGAGCAGAAGCATTTAT+AGG	+	chr2.3:16897629-16897648	MS.gene90155:CDS	35.0%
	CATGCTAATGGAAGGTGATT+TGG	+	chr2.3:16897772-16897791	MS.gene90155:CDS	40.0%
	CTACGGGTTTCATTTGCTTA+TGG	+	chr2.3:16897661-16897680	MS.gene90155:CDS	40.0%
	TTGTTTCATGCTTGGTATGG+AGG	+	chr2.3:16897727-16897746	MS.gene90155:CDS	40.0%
!	CTAATGGAAGGTGATTTGGA+TGG	+	chr2.3:16897776-16897795	MS.gene90155:CDS	40.0%
	CTCGTGTACATGCTAATGGA+AGG	+	chr2.3:16897764-16897783	MS.gene90155:CDS	45.0%
	TGAGCTCGTGTACATGCTAA+TGG	+	chr2.3:16897760-16897779	MS.gene90155:CDS	45.0%
	TGAGGACTACTTTCCATCCA+TGG	+	chr2.3:16897598-16897617	MS.gene90155:CDS	45.0%
!	GAAGGTGATTTGGATGGAGA+TGG	+	chr2.3:16897782-16897801	MS.gene90155:CDS	45.0%
	CATGCTTGGTATGGAGGTGA+GGG	+	chr2.3:16897733-16897752	MS.gene90155:CDS	50.0%
	CCATCCATGGTTGCGAGTTT+AGG	+	chr2.3:16897611-16897630	MS.gene90155:CDS	50.0%
	CCTAAACTCGCAACCATGGA+TGG	-	chr2.3:16897614-16897633	None:intergenic	50.0%
	TCATGCTTGGTATGGAGGTG+AGG	+	chr2.3:16897732-16897751	MS.gene90155:CDS	50.0%
	TGCTCCTAAACTCGCAACCA+TGG	-	chr2.3:16897618-16897637	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	16897572	16897916	16897572	ID=MS.gene90155
chr2.3	mRNA	16897572	16897916	16897572	ID=MS.gene90155.t1;Parent=MS.gene90155
chr2.3	exon	16897572	16897916	16897572	ID=MS.gene90155.t1.exon1;Parent=MS.gene90155.t1
chr2.3	CDS	16897572	16897916	16897572	ID=cds.MS.gene90155.t1;Parent=MS.gene90155.t1

Gene Sequence	>MS.gene90155 ATGGAGTTGGATTTTGATATGGATTTTGAGGACTACTTTCCATCCATGGTTGCGAGTTTAGGAGCAGAAGCATTTATAGGTGAACTTTGCTACGGGTTTCATTTGCTTATGGATGTCAACAAGGGTCTTATAACATTTGAGAGTTTGAAGATGAATTGTTTCATGCTTGGTATGGAGGTGAGGGATGATGAGCTCGTGTACATGCTAATGGAAGGTGATTTGGATGGAGATGGTGCTTTAAATCCAATGGAGTTTTGCATTCTCATGTTTAGGTTGAGTCCATGTTTAATGGATGGACCTAAAATATGCACAAATCTTCAAGGTGTGAATCCAATGTTAATGTGA
Protein sequence	>MS.gene90155.t1 MELDFDMDFEDYFPSMVASLGAEAFIGELCYGFHLLMDVNKGLITFESLKMNCFMLGMEVRDDELVYMLMEGDLDGDGALNPMEFCILMFRLSPCLMDGPKICTNLQGVNPMLM