Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90261.t1 | XP_003629262.1 | 94.4 | 125 | 7 | 0 | 1 | 125 | 1 | 125 | 2.00E-58 | 235 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90261.t1 | Q8L7E9 | 81.6 | 125 | 23 | 0 | 1 | 125 | 1 | 125 | 1.8e-52 | 206.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90261.t1 | G7LB65 | 94.4 | 125 | 7 | 0 | 1 | 125 | 1 | 125 | 1.4e-58 | 235.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056638 | MS.gene90261 | 0.820071 | 8.32E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90261.t1 | MTR_8g075150 | 94.400 | 125 | 7 | 0 | 1 | 125 | 1 | 125 | 2.00e-81 | 245 |
| MS.gene90261.t1 | MTR_5g022670 | 82.927 | 123 | 21 | 0 | 3 | 125 | 2 | 124 | 2.03e-71 | 219 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90261.t1 | AT4G35920 | 81.600 | 125 | 23 | 0 | 1 | 125 | 1 | 125 | 4.38e-70 | 216 |
| MS.gene90261.t1 | AT4G35920 | 81.600 | 125 | 23 | 0 | 1 | 125 | 1 | 125 | 4.38e-70 | 216 |
| MS.gene90261.t1 | AT4G35920 | 81.600 | 125 | 23 | 0 | 1 | 125 | 1 | 125 | 4.38e-70 | 216 |
| MS.gene90261.t1 | AT4G35920 | 81.600 | 125 | 23 | 0 | 1 | 125 | 1 | 125 | 4.38e-70 | 216 |
| MS.gene90261.t1 | AT4G35920 | 81.600 | 125 | 23 | 0 | 1 | 125 | 1 | 125 | 4.38e-70 | 216 |
| MS.gene90261.t1 | AT2G17780 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 7.83e-59 | 184 |
| MS.gene90261.t1 | AT2G17780 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 3.87e-58 | 184 |
| MS.gene90261.t1 | AT2G17780 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 6.65e-58 | 184 |
| MS.gene90261.t1 | AT2G17780 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 6.65e-58 | 184 |
| MS.gene90261.t1 | AT2G17780 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 6.65e-58 | 184 |
| MS.gene90261.t1 | AT2G17780 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 6.65e-58 | 184 |
| MS.gene90261.t1 | AT2G17780 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 6.65e-58 | 184 |
| MS.gene90261.t1 | AT2G17780 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 7.33e-58 | 184 |
| MS.gene90261.t1 | AT2G17780 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 7.33e-58 | 184 |
| MS.gene90261.t1 | AT2G17780 | 71.200 | 125 | 36 | 0 | 1 | 125 | 1 | 125 | 7.96e-58 | 184 |
Find 23 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTAGAGGCTCTCGGGTTTC+AGG | 0.140533 | 4.2:+20241415 | None:intergenic |
| CTGCATCTAAGCCTATTAAT+TGG | 0.182088 | 4.2:+20241575 | None:intergenic |
| TTTGAGAAGGTCTTCTATTT+TGG | 0.212238 | 4.2:-20241377 | MS.gene90261:CDS |
| GGAACATTGTTTATCAATTT+AGG | 0.249469 | 4.2:-20241306 | MS.gene90261:CDS |
| GCAAATGTAGCCCAATTAAT+AGG | 0.272486 | 4.2:-20241586 | MS.gene90261:CDS |
| GCATCTTCATGGGAACAATT+TGG | 0.278027 | 4.2:-20241616 | MS.gene90261:CDS |
| GCACAACATCTTAAGTTGAT+TGG | 0.319124 | 4.2:-20241481 | MS.gene90261:CDS |
| TAGATGCAGTAAAGTTGATT+AGG | 0.362979 | 4.2:-20241561 | MS.gene90261:CDS |
| CACAACATCTTAAGTTGATT+GGG | 0.378407 | 4.2:-20241480 | MS.gene90261:CDS |
| CTAGTTGTTCTAGAGGCTCT+CGG | 0.379192 | 4.2:+20241407 | None:intergenic |
| GATATAGAATGGCATCTTCA+TGG | 0.416346 | 4.2:-20241627 | None:intergenic |
| TGCATCTAAGCCTATTAATT+GGG | 0.419039 | 4.2:+20241576 | None:intergenic |
| CTAGAGGCTCTCGGGTTTCA+GGG | 0.434211 | 4.2:+20241416 | None:intergenic |
| CTATCTTTATTTGCTCGCCA+TGG | 0.439513 | 4.2:-20241332 | MS.gene90261:CDS |
| TATCTTTATTTGCTCGCCAT+GGG | 0.488601 | 4.2:-20241331 | MS.gene90261:CDS |
| TAGTTGTTCTAGAGGCTCTC+GGG | 0.496041 | 4.2:+20241408 | None:intergenic |
| TTTATTTGCTCGCCATGGGA+TGG | 0.519477 | 4.2:-20241327 | MS.gene90261:CDS |
| ATATAGAATGGCATCTTCAT+GGG | 0.542232 | 4.2:-20241626 | None:intergenic |
| GCATCTTCTAGTTGTTCTAG+AGG | 0.556880 | 4.2:+20241400 | None:intergenic |
| CATTGTTTATCAATTTAGGA+AGG | 0.567874 | 4.2:-20241302 | MS.gene90261:CDS |
| ATAAACAATGTTCCATCCCA+TGG | 0.583807 | 4.2:+20241315 | None:intergenic |
| AACTAGAAGATGCTTTGAGA+AGG | 0.613004 | 4.2:-20241390 | MS.gene90261:CDS |
| CGAAAGCAGCAAATACAGCA+AGG | 0.638455 | 4.2:-20241531 | MS.gene90261:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CATTGTTTATCAATTTAGGA+AGG | - | chr4.2:20241578-20241597 | MS.gene90261:CDS | 25.0% |
| ! | GGAACATTGTTTATCAATTT+AGG | - | chr4.2:20241574-20241593 | MS.gene90261:CDS | 25.0% |
| TGCATCTAAGCCTATTAATT+GGG | + | chr4.2:20241307-20241326 | None:intergenic | 30.0% | |
| ! | TAGATGCAGTAAAGTTGATT+AGG | - | chr4.2:20241319-20241338 | MS.gene90261:CDS | 30.0% |
| ! | TTTGAGAAGGTCTTCTATTT+TGG | - | chr4.2:20241503-20241522 | MS.gene90261:CDS | 30.0% |
| !! | CACAACATCTTAAGTTGATT+GGG | - | chr4.2:20241400-20241419 | MS.gene90261:CDS | 30.0% |
| !!! | TATTTTGGTCAATAGTTGTC+AGG | - | chr4.2:20241518-20241537 | MS.gene90261:CDS | 30.0% |
| ATAAACAATGTTCCATCCCA+TGG | + | chr4.2:20241568-20241587 | None:intergenic | 35.0% | |
| CTGCATCTAAGCCTATTAAT+TGG | + | chr4.2:20241308-20241327 | None:intergenic | 35.0% | |
| GCAAATGTAGCCCAATTAAT+AGG | - | chr4.2:20241294-20241313 | MS.gene90261:CDS | 35.0% | |
| ! | TATCTTTATTTGCTCGCCAT+GGG | - | chr4.2:20241549-20241568 | MS.gene90261:CDS | 35.0% |
| !! | AACTAGAAGATGCTTTGAGA+AGG | - | chr4.2:20241490-20241509 | MS.gene90261:CDS | 35.0% |
| !! | GCACAACATCTTAAGTTGAT+TGG | - | chr4.2:20241399-20241418 | MS.gene90261:CDS | 35.0% |
| GCATCTTCATGGGAACAATT+TGG | - | chr4.2:20241264-20241283 | MS.gene90261:CDS | 40.0% | |
| ! | CTATCTTTATTTGCTCGCCA+TGG | - | chr4.2:20241548-20241567 | MS.gene90261:CDS | 40.0% |
| ! | GCATCTTCTAGTTGTTCTAG+AGG | + | chr4.2:20241483-20241502 | None:intergenic | 40.0% |
| CGAAAGCAGCAAATACAGCA+AGG | - | chr4.2:20241349-20241368 | MS.gene90261:CDS | 45.0% | |
| TTTATTTGCTCGCCATGGGA+TGG | - | chr4.2:20241553-20241572 | MS.gene90261:CDS | 45.0% | |
| !! | CTAGTTGTTCTAGAGGCTCT+CGG | + | chr4.2:20241476-20241495 | None:intergenic | 45.0% |
| !! | TAGTTGTTCTAGAGGCTCTC+GGG | + | chr4.2:20241475-20241494 | None:intergenic | 45.0% |
| CTAGAGGCTCTCGGGTTTCA+GGG | + | chr4.2:20241467-20241486 | None:intergenic | 55.0% | |
| TCTAGAGGCTCTCGGGTTTC+AGG | + | chr4.2:20241468-20241487 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 20241261 | 20241641 | 20241261 | ID=MS.gene90261 |
| chr4.2 | mRNA | 20241261 | 20241641 | 20241261 | ID=MS.gene90261.t1;Parent=MS.gene90261 |
| chr4.2 | exon | 20241261 | 20241641 | 20241261 | ID=MS.gene90261.t1.exon1;Parent=MS.gene90261.t1 |
| chr4.2 | CDS | 20241261 | 20241641 | 20241261 | ID=cds.MS.gene90261.t1;Parent=MS.gene90261.t1 |
| Gene Sequence |
| Protein sequence |
