Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90335.t1 | XP_003616258.2 | 94.7 | 225 | 12 | 0 | 1 | 225 | 1 | 225 | 2.00E-125 | 458.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90335.t1 | G7KG29 | 94.7 | 225 | 12 | 0 | 1 | 225 | 1 | 225 | 1.4e-125 | 458.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054801 | MS.gene90335 | 0.802689 | 4.98E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene90335 | 0.829166 | 5.99E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90335.t1 | MTR_5g077900 | 94.248 | 226 | 13 | 0 | 1 | 226 | 1 | 226 | 2.41e-162 | 447 |
| MS.gene90335.t1 | MTR_0002s1270 | 93.805 | 226 | 14 | 0 | 1 | 226 | 175 | 400 | 2.52e-161 | 452 |
| MS.gene90335.t1 | MTR_7g046380 | 94.884 | 215 | 11 | 0 | 1 | 215 | 183 | 397 | 2.05e-155 | 437 |
| MS.gene90335.t1 | MTR_2g092960 | 37.443 | 219 | 131 | 4 | 6 | 223 | 1003 | 1216 | 5.14e-42 | 152 |
| MS.gene90335.t1 | MTR_0462s0020 | 33.645 | 214 | 135 | 3 | 12 | 222 | 82 | 291 | 7.38e-39 | 136 |
| MS.gene90335.t1 | MTR_8g051620 | 52.294 | 109 | 52 | 0 | 1 | 109 | 1 | 109 | 1.71e-38 | 130 |
| MS.gene90335.t1 | MTR_8g051620 | 52.778 | 108 | 51 | 0 | 1 | 108 | 1 | 108 | 2.07e-38 | 129 |
| MS.gene90335.t1 | MTR_0057s0200 | 96.610 | 59 | 2 | 0 | 168 | 226 | 1 | 59 | 4.15e-37 | 124 |
| MS.gene90335.t1 | MTR_8g017000 | 35.437 | 206 | 126 | 3 | 24 | 226 | 488 | 689 | 2.58e-35 | 132 |
| MS.gene90335.t1 | MTR_2g037270 | 35.437 | 206 | 126 | 3 | 24 | 226 | 488 | 689 | 3.43e-35 | 132 |
| MS.gene90335.t1 | MTR_4g066320 | 85.246 | 61 | 8 | 1 | 165 | 224 | 59 | 119 | 7.80e-33 | 115 |
| MS.gene90335.t1 | MTR_3g011560 | 32.843 | 204 | 121 | 3 | 24 | 226 | 488 | 676 | 2.17e-31 | 121 |
| MS.gene90335.t1 | MTR_4g066310 | 83.673 | 49 | 8 | 0 | 1 | 49 | 151 | 199 | 1.60e-22 | 91.3 |
| MS.gene90335.t1 | MTR_3g028430 | 74.074 | 54 | 5 | 1 | 173 | 226 | 15 | 59 | 1.61e-19 | 79.3 |
| MS.gene90335.t1 | MTR_4g066300 | 88.235 | 34 | 4 | 0 | 164 | 197 | 5 | 38 | 1.61e-14 | 66.2 |
| MS.gene90335.t1 | MTR_2g086200 | 62.500 | 48 | 18 | 0 | 177 | 224 | 185 | 232 | 2.58e-13 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCGAAAGTGTTTGGGATTT+TGG | 0.107550 | 7.4:+43342526 | MS.gene90335:CDS |
| TTGCCATTAGGTTGCTTTCT+TGG | 0.248489 | 7.4:-43343122 | None:intergenic |
| TCATTGCTTCATTAAGATCT+TGG | 0.330442 | 7.4:-43342817 | None:intergenic |
| TGGTTAACACAGAGCTTTAG+AGG | 0.331097 | 7.4:+43342936 | MS.gene90335:intron |
| AACTGTCTAATACACTTTGC+TGG | 0.367487 | 7.4:-43342233 | None:intergenic |
| GCAATAGAAAGAAGGCAAAA+TGG | 0.368092 | 7.4:+43342977 | MS.gene90335:CDS |
| AAAATAGATCGAAAGTGTTT+GGG | 0.378893 | 7.4:+43342519 | MS.gene90335:CDS |
| AGAGGTTCATCAACTTGCTC+AGG | 0.406730 | 7.4:+43342954 | MS.gene90335:CDS |
| GTATTTGGCTCATGTTCTAA+TGG | 0.408074 | 7.4:-43342194 | None:intergenic |
| GACAACTAAACTATTGTCCT+TGG | 0.409668 | 7.4:-43342780 | None:intergenic |
| TATCCACAATCATTGCCATT+AGG | 0.411955 | 7.4:-43343134 | None:intergenic |
| TATACTTACTTAAATAGATA+TGG | 0.415179 | 7.4:-43342656 | None:intergenic |
| AAAGTGTATTAGACAGTTGT+TGG | 0.445582 | 7.4:+43342238 | MS.gene90335:CDS |
| TAGTACTAAAACTAAAGTTC+AGG | 0.451652 | 7.4:+43342583 | MS.gene90335:CDS |
| TGGATTAAAATCCAAGCATA+CGG | 0.460463 | 7.4:-43342552 | None:intergenic |
| TCTTGACTTATATAACGCTT+AGG | 0.471488 | 7.4:+43343389 | MS.gene90335:CDS |
| CTGCTAATATTACTAAGTCT+TGG | 0.472781 | 7.4:+43343189 | MS.gene90335:CDS |
| GTATGATTTGCGAAACCAAT+GGG | 0.494818 | 7.4:+43343356 | MS.gene90335:CDS |
| TAGCCAAGAAAGCAACCTAA+TGG | 0.495668 | 7.4:+43343119 | MS.gene90335:intron |
| CAACCTAATGGCAATGATTG+TGG | 0.497368 | 7.4:+43343131 | MS.gene90335:CDS |
| CAAGAAAACAAGTTGCCCAT+TGG | 0.504204 | 7.4:-43343371 | None:intergenic |
| GTTAATAATTATTTGTCCCA+AGG | 0.509187 | 7.4:+43342763 | MS.gene90335:CDS |
| GGATTAAAATCCAAGCATAC+GGG | 0.511064 | 7.4:-43342551 | None:intergenic |
| ACAACTAAACTATTGTCCTT+GGG | 0.519949 | 7.4:-43342779 | None:intergenic |
| TCATCTTCAGTTAATGTCGA+TGG | 0.549468 | 7.4:-43343330 | None:intergenic |
| AGTTGTTGGTGGGTAATGCT+TGG | 0.552427 | 7.4:+43342252 | MS.gene90335:CDS |
| GAGGTTCATCAACTTGCTCA+GGG | 0.558482 | 7.4:+43342955 | MS.gene90335:CDS |
| GTGTATTAGACAGTTGTTGG+TGG | 0.561452 | 7.4:+43342241 | MS.gene90335:CDS |
| TGCTCAGGGCAATAGAAAGA+AGG | 0.563067 | 7.4:+43342969 | MS.gene90335:CDS |
| TGTATTAGACAGTTGTTGGT+GGG | 0.569307 | 7.4:+43342242 | MS.gene90335:CDS |
| TGTATGATTTGCGAAACCAA+TGG | 0.592485 | 7.4:+43343355 | MS.gene90335:CDS |
| TCTACGTGTGCAGAGGACAC+TGG | 0.601997 | 7.4:+43342738 | MS.gene90335:intron |
| TTCAGGAACACATACAAACT+AGG | 0.603672 | 7.4:+43342600 | MS.gene90335:CDS |
| TAATATTACTAAGTCTTGGA+TGG | 0.604101 | 7.4:+43343193 | MS.gene90335:CDS |
| GATCTTGGTGCATTGAACAA+AGG | 0.604500 | 7.4:-43342802 | None:intergenic |
| AACATGAGCCAAATACCGCA+AGG | 0.641032 | 7.4:+43342201 | MS.gene90335:CDS |
| TATTACTAAGTCTTGGATGG+AGG | 0.643493 | 7.4:+43343196 | MS.gene90335:CDS |
| GATTAAAATCCAAGCATACG+GGG | 0.693850 | 7.4:-43342550 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACTAATCACTATATAAAAA+TGG | + | chr7.4:43343085-43343104 | MS.gene90335:intron | 15.0% |
| !! | TATACTTACTTAAATAGATA+TGG | - | chr7.4:43342659-43342678 | None:intergenic | 15.0% |
| !!! | CTTTGATGATTTTTTTAATT+TGG | + | chr7.4:43342916-43342935 | MS.gene90335:intron | 15.0% |
| !!! | TGTTAATTTTAAATTTGTCT+AGG | + | chr7.4:43342375-43342394 | MS.gene90335:intron | 15.0% |
| !! | AACACTTAAATATTGAATTG+TGG | - | chr7.4:43342359-43342378 | None:intergenic | 20.0% |
| !! | ATCGAATACTATTATCAATA+TGG | + | chr7.4:43342416-43342435 | MS.gene90335:intron | 20.0% |
| !!! | CTTTTGTACTATTATCAATA+TGG | + | chr7.4:43342320-43342339 | MS.gene90335:intron | 20.0% |
| ! | ACGTAGAAGAACAATTATTA+AGG | - | chr7.4:43342725-43342744 | None:intergenic | 25.0% |
| ! | GTTAATAATTATTTGTCCCA+AGG | + | chr7.4:43342763-43342782 | MS.gene90335:CDS | 25.0% |
| ! | TAATATTACTAAGTCTTGGA+TGG | + | chr7.4:43343193-43343212 | MS.gene90335:CDS | 25.0% |
| ! | TAGTACTAAAACTAAAGTTC+AGG | + | chr7.4:43342583-43342602 | MS.gene90335:CDS | 25.0% |
| ! | TTTATTAACCTACACATTGT+AGG | + | chr7.4:43343296-43343315 | MS.gene90335:intron | 25.0% |
| !! | ACTTTTCTATTCTACAACTT+TGG | + | chr7.4:43342279-43342298 | MS.gene90335:CDS | 25.0% |
| !!! | AAAAATAGATCGAAAGTGTT+TGG | + | chr7.4:43342518-43342537 | MS.gene90335:CDS | 25.0% |
| !!! | AAAATAGATCGAAAGTGTTT+GGG | + | chr7.4:43342519-43342538 | MS.gene90335:CDS | 25.0% |
| !!! | GTTATACATTTTAGTGTTGT+AGG | - | chr7.4:43342897-43342916 | None:intergenic | 25.0% |
| !!! | GTTTTAGTACTATGATCTTT+TGG | - | chr7.4:43342575-43342594 | None:intergenic | 25.0% |
| AAAGTGTATTAGACAGTTGT+TGG | + | chr7.4:43342238-43342257 | MS.gene90335:CDS | 30.0% | |
| AATCTACACCATACTTACTT+TGG | - | chr7.4:43343010-43343029 | None:intergenic | 30.0% | |
| ACAACTAAACTATTGTCCTT+GGG | - | chr7.4:43342782-43342801 | None:intergenic | 30.0% | |
| ATAAAAAACCTTGCGGTATT+TGG | - | chr7.4:43342212-43342231 | None:intergenic | 30.0% | |
| ATCTACACCATACTTACTTT+GGG | - | chr7.4:43343009-43343028 | None:intergenic | 30.0% | |
| ATTGAATACCTACAATGTGT+AGG | - | chr7.4:43343307-43343326 | None:intergenic | 30.0% | |
| CTGCTAATATTACTAAGTCT+TGG | + | chr7.4:43343189-43343208 | MS.gene90335:CDS | 30.0% | |
| TCATTGCTTCATTAAGATCT+TGG | - | chr7.4:43342820-43342839 | None:intergenic | 30.0% | |
| TGGATTAAAATCCAAGCATA+CGG | - | chr7.4:43342555-43342574 | None:intergenic | 30.0% | |
| AACTGTCTAATACACTTTGC+TGG | - | chr7.4:43342236-43342255 | None:intergenic | 35.0% | |
| CTTTCTTGGCTACAAATGAT+TGG | - | chr7.4:43343111-43343130 | None:intergenic | 35.0% | |
| GACAACTAAACTATTGTCCT+TGG | - | chr7.4:43342783-43342802 | None:intergenic | 35.0% | |
| GATTAAAATCCAAGCATACG+GGG | - | chr7.4:43342553-43342572 | None:intergenic | 35.0% | |
| GCAATAGAAAGAAGGCAAAA+TGG | + | chr7.4:43342977-43342996 | MS.gene90335:CDS | 35.0% | |
| GGATTAAAATCCAAGCATAC+GGG | - | chr7.4:43342554-43342573 | None:intergenic | 35.0% | |
| TATCCACAATCATTGCCATT+AGG | - | chr7.4:43343137-43343156 | None:intergenic | 35.0% | |
| TATTACTAAGTCTTGGATGG+AGG | + | chr7.4:43343196-43343215 | MS.gene90335:CDS | 35.0% | |
| TCATCTTCAGTTAATGTCGA+TGG | - | chr7.4:43343333-43343352 | None:intergenic | 35.0% | |
| TGCTGGAATAAAAAACCTTG+CGG | - | chr7.4:43342219-43342238 | None:intergenic | 35.0% | |
| TGTATTAGACAGTTGTTGGT+GGG | + | chr7.4:43342242-43342261 | MS.gene90335:CDS | 35.0% | |
| TTCAGGAACACATACAAACT+AGG | + | chr7.4:43342600-43342619 | MS.gene90335:CDS | 35.0% | |
| ! | GTATGATTTGCGAAACCAAT+GGG | + | chr7.4:43343356-43343375 | MS.gene90335:CDS | 35.0% |
| ! | GTATTTGGCTCATGTTCTAA+TGG | - | chr7.4:43342197-43342216 | None:intergenic | 35.0% |
| ! | TGTATGATTTGCGAAACCAA+TGG | + | chr7.4:43343355-43343374 | MS.gene90335:CDS | 35.0% |
| !!! | ATCGAAAGTGTTTGGGATTT+TGG | + | chr7.4:43342526-43342545 | MS.gene90335:CDS | 35.0% |
| CAACCTAATGGCAATGATTG+TGG | + | chr7.4:43343131-43343150 | MS.gene90335:CDS | 40.0% | |
| CAAGAAAACAAGTTGCCCAT+TGG | - | chr7.4:43343374-43343393 | None:intergenic | 40.0% | |
| GTGTATTAGACAGTTGTTGG+TGG | + | chr7.4:43342241-43342260 | MS.gene90335:CDS | 40.0% | |
| GTTTCGTCCCAAAGTAAGTA+TGG | + | chr7.4:43342999-43343018 | MS.gene90335:intron | 40.0% | |
| TAGCCAAGAAAGCAACCTAA+TGG | + | chr7.4:43343119-43343138 | MS.gene90335:intron | 40.0% | |
| ! | GATCTTGGTGCATTGAACAA+AGG | - | chr7.4:43342805-43342824 | None:intergenic | 40.0% |
| ! | TGGTTAACACAGAGCTTTAG+AGG | + | chr7.4:43342936-43342955 | MS.gene90335:intron | 40.0% |
| ! | TTGCCATTAGGTTGCTTTCT+TGG | - | chr7.4:43343125-43343144 | None:intergenic | 40.0% |
| AACATGAGCCAAATACCGCA+AGG | + | chr7.4:43342201-43342220 | MS.gene90335:CDS | 45.0% | |
| TGCTCAGGGCAATAGAAAGA+AGG | + | chr7.4:43342969-43342988 | MS.gene90335:CDS | 45.0% | |
| TTGTTCTTCTACGTGTGCAG+AGG | + | chr7.4:43342731-43342750 | MS.gene90335:intron | 45.0% | |
| ! | AGAGGTTCATCAACTTGCTC+AGG | + | chr7.4:43342954-43342973 | MS.gene90335:CDS | 45.0% |
| ! | AGTTGTTGGTGGGTAATGCT+TGG | + | chr7.4:43342252-43342271 | MS.gene90335:CDS | 45.0% |
| ! | GAGGTTCATCAACTTGCTCA+GGG | + | chr7.4:43342955-43342974 | MS.gene90335:CDS | 45.0% |
| !! | AAAAGTAATTAATATTAATA+AGG | - | chr7.4:43343279-43343298 | None:intergenic | 5.0% |
| ! | GATTTTGGACCCCGTATGCT+TGG | + | chr7.4:43342541-43342560 | MS.gene90335:CDS | 50.0% |
| TCTACGTGTGCAGAGGACAC+TGG | + | chr7.4:43342738-43342757 | MS.gene90335:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 43342161 | 43343410 | 43342161 | ID=MS.gene90335 |
| chr7.4 | mRNA | 43342161 | 43343410 | 43342161 | ID=MS.gene90335.t1;Parent=MS.gene90335 |
| chr7.4 | exon | 43342161 | 43342306 | 43342161 | ID=MS.gene90335.t1.exon1;Parent=MS.gene90335.t1 |
| chr7.4 | CDS | 43342161 | 43342306 | 43342161 | ID=cds.MS.gene90335.t1;Parent=MS.gene90335.t1 |
| chr7.4 | exon | 43342493 | 43342669 | 43342493 | ID=MS.gene90335.t1.exon2;Parent=MS.gene90335.t1 |
| chr7.4 | CDS | 43342493 | 43342669 | 43342493 | ID=cds.MS.gene90335.t1;Parent=MS.gene90335.t1 |
| chr7.4 | exon | 43342751 | 43342855 | 43342751 | ID=MS.gene90335.t1.exon3;Parent=MS.gene90335.t1 |
| chr7.4 | CDS | 43342751 | 43342855 | 43342751 | ID=cds.MS.gene90335.t1;Parent=MS.gene90335.t1 |
| chr7.4 | exon | 43342948 | 43343011 | 43342948 | ID=MS.gene90335.t1.exon4;Parent=MS.gene90335.t1 |
| chr7.4 | CDS | 43342948 | 43343011 | 43342948 | ID=cds.MS.gene90335.t1;Parent=MS.gene90335.t1 |
| chr7.4 | exon | 43343122 | 43343217 | 43343122 | ID=MS.gene90335.t1.exon5;Parent=MS.gene90335.t1 |
| chr7.4 | CDS | 43343122 | 43343217 | 43343122 | ID=cds.MS.gene90335.t1;Parent=MS.gene90335.t1 |
| chr7.4 | exon | 43343318 | 43343410 | 43343318 | ID=MS.gene90335.t1.exon6;Parent=MS.gene90335.t1 |
| chr7.4 | CDS | 43343318 | 43343410 | 43343318 | ID=cds.MS.gene90335.t1;Parent=MS.gene90335.t1 |
| Gene Sequence |
| Protein sequence |
