AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90654

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90654.t1 MTR_1g023760 96.401 778 28 0 1 778 1 778 0.0 1530
MS.gene90654.t1 MTR_8g466020 73.485 132 25 1 48 179 19 140 1.23e-53 187
MS.gene90654.t1 MTR_7g007170 79.592 98 20 0 84 181 11 108 3.12e-46 164
MS.gene90654.t1 MTR_1g023925 44.706 170 20 3 557 724 1 98 7.37e-28 108
MS.gene90654.t1 MTR_5g038620 37.313 134 83 1 126 259 121 253 5.46e-17 85.9
MS.gene90654.t1 MTR_4g108140 35.714 140 84 2 124 259 99 236 3.06e-16 83.2
MS.gene90654.t1 MTR_4g108140 35.714 140 84 2 124 259 100 237 3.26e-16 83.2
MS.gene90654.t1 MTR_3g091560 36.047 172 66 6 124 261 112 273 4.32e-15 79.0
MS.gene90654.t1 MTR_4g050480 32.121 165 82 4 124 262 97 257 3.49e-14 75.9
MS.gene90654.t1 MTR_4g050480 32.121 165 82 4 124 262 96 256 3.66e-14 75.9
MS.gene90654.t1 MTR_8g024540 59.649 57 23 0 126 182 129 185 4.54e-14 76.3
MS.gene90654.t1 MTR_0019s0070 33.333 156 88 7 107 260 106 247 1.32e-12 71.2
MS.gene90654.t1 MTR_1g101330 28.631 241 143 8 30 260 25 246 2.01e-12 70.5
MS.gene90654.t1 MTR_8g014580 49.254 67 34 0 126 192 128 194 2.66e-12 70.1
MS.gene90654.t1 MTR_1g041430 56.604 53 23 0 124 176 111 163 4.36e-12 69.3
MS.gene90654.t1 MTR_7g114700 50.769 65 32 0 124 188 135 199 5.85e-12 68.9
MS.gene90654.t1 MTR_1g095740 28.834 163 90 3 124 260 141 303 1.22e-11 68.2
MS.gene90654.t1 MTR_1g007920 56.364 55 24 0 121 175 94 148 2.17e-11 67.4
MS.gene90654.t1 MTR_0047s0040 43.210 81 44 1 117 197 94 172 2.38e-11 67.0
MS.gene90654.t1 MTR_1g007920 56.364 55 24 0 121 175 119 173 2.95e-11 67.0
MS.gene90654.t1 MTR_1g066800 28.302 159 92 2 124 260 116 274 4.88e-11 66.2
MS.gene90654.t1 MTR_1g066800 28.302 159 92 2 124 260 121 279 5.30e-11 66.2
MS.gene90654.t1 MTR_1g103070 44.444 72 39 1 124 195 136 206 5.62e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90654.t1 AT1G19870 36.984 703 283 29 113 773 21 605 2.96e-95 309
MS.gene90654.t1 AT1G19870 36.984 703 283 29 113 773 204 788 3.43e-93 309
MS.gene90654.t1 AT1G18840 36.364 176 94 6 91 259 81 245 6.08e-22 101
MS.gene90654.t1 AT1G18840 36.364 176 94 6 91 259 81 245 6.08e-22 101
MS.gene90654.t1 AT1G18840 36.364 176 94 6 91 259 81 245 6.08e-22 101
MS.gene90654.t1 AT1G74690 40.288 139 78 3 124 259 113 249 7.73e-21 97.8
MS.gene90654.t1 AT1G14380 37.589 141 77 3 124 259 94 228 1.05e-20 97.8
MS.gene90654.t1 AT1G14380 37.589 141 77 3 124 259 94 228 1.05e-20 97.8
MS.gene90654.t1 AT1G14380 37.589 141 77 3 124 259 94 228 1.05e-20 97.8
MS.gene90654.t1 AT1G14380 37.589 141 77 3 124 259 94 228 1.32e-20 97.1
MS.gene90654.t1 AT1G14380 37.589 141 77 3 124 259 94 228 1.32e-20 97.1
MS.gene90654.t1 AT1G14380 37.589 141 77 3 124 259 94 228 1.32e-20 97.1
MS.gene90654.t1 AT1G14380 37.589 141 77 3 124 259 94 228 1.51e-20 97.1
MS.gene90654.t1 AT5G03040 29.630 162 87 4 124 260 115 274 4.81e-15 79.0
MS.gene90654.t1 AT5G03040 29.630 162 87 4 124 260 115 274 4.81e-15 79.0
MS.gene90654.t1 AT5G03040 29.630 162 87 4 124 260 115 274 4.81e-15 79.0
MS.gene90654.t1 AT3G49260 51.471 68 33 0 124 191 120 187 6.38e-15 78.6
MS.gene90654.t1 AT3G49260 51.471 68 33 0 124 191 120 187 6.38e-15 78.6
MS.gene90654.t1 AT3G49260 51.471 68 33 0 124 191 120 187 6.38e-15 78.6
MS.gene90654.t1 AT3G49260 51.471 68 33 0 124 191 120 187 6.88e-15 78.6
MS.gene90654.t1 AT4G14750 31.111 225 118 6 1 214 1 199 1.28e-14 77.0
MS.gene90654.t1 AT2G02790 28.497 193 134 2 67 259 53 241 1.55e-14 77.8
MS.gene90654.t1 AT2G02790 28.497 193 134 2 67 259 53 241 1.55e-14 77.8
MS.gene90654.t1 AT3G52290 31.875 160 84 4 124 260 108 265 1.70e-14 77.0
MS.gene90654.t1 AT2G02790 28.804 184 130 1 76 259 59 241 1.79e-14 77.8
MS.gene90654.t1 AT1G01110 55.072 69 30 1 126 194 123 190 2.49e-14 77.0
MS.gene90654.t1 AT4G14750 31.111 225 118 6 1 214 60 258 4.32e-14 75.9
MS.gene90654.t1 AT4G14750 31.111 225 118 6 1 214 60 258 4.39e-14 75.9
MS.gene90654.t1 AT4G00820 53.623 69 31 1 126 194 134 201 9.29e-14 75.1
MS.gene90654.t1 AT3G51380 53.968 63 29 0 124 186 37 99 4.22e-13 66.6
MS.gene90654.t1 AT4G10640 35.260 173 76 6 19 189 27 165 4.74e-13 72.4
MS.gene90654.t1 AT3G22190 30.435 161 88 3 126 262 90 250 9.49e-13 71.6
MS.gene90654.t1 AT3G22190 30.435 161 88 3 126 262 90 250 9.49e-13 71.6
MS.gene90654.t1 AT3G22190 30.435 161 88 3 126 262 90 250 9.49e-13 71.6
MS.gene90654.t1 AT3G22190 30.435 161 88 3 126 262 90 250 9.49e-13 71.6
MS.gene90654.t1 AT1G72670 30.097 206 96 7 96 260 57 255 1.35e-12 70.9
MS.gene90654.t1 AT2G26180 57.692 52 22 0 124 175 84 135 2.80e-11 67.0
MS.gene90654.t1 AT3G09710 30.409 171 92 7 124 269 108 276 4.44e-11 66.2
MS.gene90654.t1 AT5G13460 32.164 171 99 6 107 260 92 262 4.56e-11 66.2

Find 160 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAATCTACTTCTTGTTTA+AGG 0.127404 1.1:+1081069 MS.gene90654:CDS
TTGATTCCATTATTTCATTT+TGG 0.176997 1.1:-1083356 None:intergenic
GGCTTCAATTAGAGGATATT+TGG 0.187454 1.1:+1082298 MS.gene90654:CDS
GAAGGTGTTCCTTGAGATTC+AGG 0.211542 1.1:-1084068 None:intergenic
TCTTGATTCTGAGGAGAATT+AGG 0.232958 1.1:-1083399 None:intergenic
GTTTCGGTGACAATTGTTTC+TGG 0.235103 1.1:-1082224 None:intergenic
GATAGTACGGTACCACTTTC+AGG 0.245019 1.1:-1083702 None:intergenic
ACTTTCAGGATCAGCATTTC+TGG 0.256182 1.1:-1083688 None:intergenic
TATCCCTTCTACTAAATCTT+TGG 0.290554 1.1:-1083784 None:intergenic
TTCTTTCCAAAATGAAATAA+TGG 0.290941 1.1:+1083350 MS.gene90654:CDS
CCCTTTGTGCCCTGCAGAGC+TGG 0.292599 1.1:-1084563 None:intergenic
GACCACATCTTCAGCAAATA+AGG 0.295588 1.1:+1082076 MS.gene90654:CDS
GATCAATTAGAAACTGTTAA+TGG 0.296217 1.1:+1083882 MS.gene90654:CDS
CAGCAATAAGTTTGCTTCTC+AGG 0.298464 1.1:+1082841 MS.gene90654:CDS
GCTGAGTTCAAATTCAAATT+CGG 0.303504 1.1:+1083557 MS.gene90654:CDS
ATTGCTACTACTATTATCTT+TGG 0.306041 1.1:-1084363 None:intergenic
GACTGTTCGGTCGTACAAAT+TGG 0.313335 1.1:-1083081 None:intergenic
GCTAATTCAAATCAAGATTC+AGG 0.330999 1.1:+1084185 MS.gene90654:CDS
GGTTCTTGATCAGTGGGTTC+AGG 0.340125 1.1:-1084338 None:intergenic
GCGAATGCAAATCATGATTC+AGG 0.353964 1.1:+1084239 MS.gene90654:CDS
CAAGCAGAATCACCTTTAGA+AGG 0.355744 1.1:-1083000 None:intergenic
AGGCTCAACAGCATGCTCTT+TGG 0.355751 1.1:-1083946 None:intergenic
ATCATCCAGGCTTCAATTAG+AGG 0.366920 1.1:+1082290 MS.gene90654:CDS
ATGATTATCACAGCAGATTC+TGG 0.367431 1.1:-1082272 None:intergenic
GTCATTATCTCCAGCTCTGC+AGG 0.369580 1.1:+1084553 MS.gene90654:CDS
CTGAGTTCAAATTCAAATTC+GGG 0.375719 1.1:+1083558 MS.gene90654:CDS
TAATTTACCTATCTCTGATT+CGG 0.378191 1.1:+1083860 MS.gene90654:CDS
CAAATCTTCTCAACAACATT+AGG 0.378376 1.1:-1082143 None:intergenic
TTGTGTAGCTTGCATGAAAC+GGG 0.383519 1.1:-1084396 None:intergenic
TGCACATCTGGACTTGATCT+TGG 0.387615 1.1:-1084452 None:intergenic
GGAAAGAGACGCAATTCATT+TGG 0.388346 1.1:+1084305 MS.gene90654:CDS
GATGCCAAAGATTTAGTAGA+AGG 0.388914 1.1:+1083780 MS.gene90654:CDS
GGAGAGATGGATGTTTGTTT+CGG 0.389207 1.1:+1083032 MS.gene90654:CDS
AACACCTTCAAGTCAGGTTT+CGG 0.391099 1.1:+1084082 MS.gene90654:CDS
TCGTACAAGGAGCTGCATTT+TGG 0.392183 1.1:-1082527 None:intergenic
AGAGGAATTTGCATTTATCT+TGG 0.400449 1.1:-1084429 None:intergenic
ATGTCAGTGTCATGCACATC+TGG 0.402507 1.1:-1084464 None:intergenic
GAGGGAAAGAAAATTACATT+TGG 0.405918 1.1:+1083480 MS.gene90654:CDS
ACCTGGTGCAACTGGTAAGC+AGG 0.406901 1.1:+1084511 MS.gene90654:CDS
TTGGTAGGTTCTTGATCAGT+GGG 0.414639 1.1:-1084344 None:intergenic
TTTCCATTCAGCTGATCCTT+AGG 0.417372 1.1:-1084287 None:intergenic
TTCTCTAATAAATGATAACT+TGG 0.417692 1.1:+1083266 MS.gene90654:CDS
AGATGCATGAGTGCTATCTT+TGG 0.419431 1.1:-1083112 None:intergenic
TTCGTGGGCACTTGGTTAGA+AGG 0.422143 1.1:+1082467 MS.gene90654:CDS
TTTGGTTTCGTCCGACTGTT+CGG 0.423888 1.1:-1083094 None:intergenic
CATTCCTTACCTGGTGCAAC+TGG 0.427428 1.1:+1084503 MS.gene90654:CDS
TTCACCGAAACCTGACTTGA+AGG 0.429554 1.1:-1084086 None:intergenic
TCAAGGAACACCTTCAAGTC+AGG 0.430241 1.1:+1084076 MS.gene90654:CDS
GGAATTGTCATATAGCTTCC+CGG 0.435431 1.1:-1084047 None:intergenic
GCATGCTCTTTGGAGTTCTC+AGG 0.444509 1.1:-1083936 None:intergenic
TCTTTCCCTCGGTCTCTAGT+TGG 0.446588 1.1:-1083467 None:intergenic
ACCTGACAGATCAGAGACTT+TGG 0.446642 1.1:+1083746 MS.gene90654:CDS
ATCCACCTTACTCTCAGTTT+CGG 0.446953 1.1:-1082240 None:intergenic
ACAAAGGGCAATGGTTCATC+TGG 0.448716 1.1:+1084578 MS.gene90654:CDS
ATTTGCATTTATCTTGGCCT+TGG 0.451725 1.1:-1084423 None:intergenic
AGAAAATTACATTTGGATCA+AGG 0.453684 1.1:+1083487 MS.gene90654:CDS
CTTCTAAAGGTGATTCTGCT+TGG 0.454777 1.1:+1083001 MS.gene90654:CDS
CCAGCTCTGCAGGGCACAAA+GGG 0.458401 1.1:+1084563 MS.gene90654:CDS
AAGGTGATTCTGCTTGGAAA+TGG 0.460534 1.1:+1083007 MS.gene90654:CDS
GAGAATAAAATCAACAAGAC+TGG 0.464101 1.1:+1084116 MS.gene90654:CDS
CTCGGATGGTAGAGGTGAAT+CGG 0.465697 1.1:-1083178 None:intergenic
GAATCTTGATTTGAATTAGC+AGG 0.467650 1.1:-1084182 None:intergenic
AATTGATCCGAATCAGAGAT+AGG 0.472689 1.1:-1083867 None:intergenic
AGCTGCTGTTCGTGGGCACT+TGG 0.480789 1.1:+1082459 MS.gene90654:CDS
GTATTCTCAACAAGCTTCAC+CGG 0.482731 1.1:+1084028 MS.gene90654:CDS
TCCAAAGTCTCTGATCTGTC+AGG 0.487160 1.1:-1083747 None:intergenic
CCTGAAAACAAGATTGATCA+TGG 0.488495 1.1:+1083813 MS.gene90654:CDS
ATTTGCATTCGCAGGAGACT+TGG 0.490435 1.1:-1084228 None:intergenic
GTCAAATGTGATCCTTCTAA+AGG 0.490498 1.1:+1082988 MS.gene90654:CDS
ATCTGCTGTGATAATCATCC+AGG 0.492717 1.1:+1082277 MS.gene90654:CDS
ATTGTGTAGCTTGCATGAAA+CGG 0.493254 1.1:-1084397 None:intergenic
AGGTTAATCACATGCGGTGG+TGG 0.495955 1.1:+1081089 MS.gene90654:CDS
CTTACCAGTTGCACCAGGTA+AGG 0.496866 1.1:-1084507 None:intergenic
TTTGGTAGGTTCTTGATCAG+TGG 0.497450 1.1:-1084345 None:intergenic
TTGCCTAAGGATCAGCTGAA+TGG 0.499267 1.1:+1084284 MS.gene90654:CDS
CTTGGAAATGGTTGGAGAGA+TGG 0.499335 1.1:+1083019 MS.gene90654:CDS
CTACTACTATTATCTTTGGT+AGG 0.501445 1.1:-1084359 None:intergenic
GAGCATGTCGTGCTCGTACA+AGG 0.502104 1.1:-1082540 None:intergenic
GGCAGTCTTCTCTTCATCCT+CGG 0.508919 1.1:-1083196 None:intergenic
AACTGAAGAATAACTTGAGA+TGG 0.509161 1.1:-1083153 None:intergenic
TGTCACCGAAACTGAGAGTA+AGG 0.509505 1.1:+1082235 MS.gene90654:CDS
GAGGATTGAAAACTAATACT+TGG 0.509672 1.1:-1082113 None:intergenic
TCATCACTTTCTTGATTCTG+AGG 0.511188 1.1:-1083408 None:intergenic
TAGCACTCATGCATCTCAAT+CGG 0.515881 1.1:+1083119 MS.gene90654:CDS
GCCGAGTGGTTTGCTCGACC+AGG 0.516424 1.1:+1083584 MS.gene90654:CDS
ATCAGCATTTCTGGAAGGAT+AGG 0.517006 1.1:-1083679 None:intergenic
AATATCCTCTAATTGAAGCC+TGG 0.517071 1.1:-1082295 None:intergenic
TCTTCATCCTCGGATGGTAG+AGG 0.519415 1.1:-1083186 None:intergenic
GAATCATGATTTGCATTCGC+AGG 0.523017 1.1:-1084236 None:intergenic
ACAAACTGAGACTGTTCTGC+AGG 0.526275 1.1:+1083998 MS.gene90654:CDS
AATAAATGATAACTTGGAAG+AGG 0.528936 1.1:+1083272 MS.gene90654:CDS
ATTCTAATGATAAACGCGGT+TGG 0.530998 1.1:+1081994 MS.gene90654:CDS
GGATCACATTTGACATGGAT+AGG 0.532828 1.1:-1082979 None:intergenic
AGAAATGCTGATCCTGAAAG+TGG 0.536855 1.1:+1083690 MS.gene90654:CDS
GAGATAGGTAAATTAGAAGT+CGG 0.539880 1.1:-1083852 None:intergenic
TTGGTTAGAAGGCATGCTGT+TGG 0.542923 1.1:+1082478 MS.gene90654:CDS
AGACGCAGGGTACTGTCTGC+TGG 0.543441 1.1:+1084149 MS.gene90654:CDS
TGATTGAGCAGCAATAAATG+AGG 0.543692 1.1:-1083523 None:intergenic
CTCCTTATTTGCTGAAGATG+TGG 0.543734 1.1:-1082078 None:intergenic
TGATTCTGCTTGGAAATGGT+TGG 0.544910 1.1:+1083011 MS.gene90654:CDS
TTGGAAAGAATCAATTTCAG+TGG 0.550366 1.1:-1083337 None:intergenic
GTGACTCAACCGATACATCC+TGG 0.551810 1.1:-1083602 None:intergenic
GAAAGAATCAATTTCAGTGG+AGG 0.553284 1.1:-1083334 None:intergenic
AGTTGAAGAAATAGATAGTA+CGG 0.556045 1.1:-1083715 None:intergenic
ATGACAATTCCTGAATCTCA+AGG 0.562140 1.1:+1084059 MS.gene90654:CDS
TGAGTTGGTATCATAGTAAG+TGG 0.562597 1.1:-1083217 None:intergenic
GTCTTCTCTTCATCCTCGGA+TGG 0.563357 1.1:-1083192 None:intergenic
TTTGCTCGACCAGGATGTAT+CGG 0.563779 1.1:+1083593 MS.gene90654:CDS
AGATCACCATGTTGTTTCTG+AGG 0.564237 1.1:+1081124 MS.gene90654:CDS
AAGTTGCAAGCTGCTGTTCG+TGG 0.564977 1.1:+1082451 MS.gene90654:CDS
TCAGCATTTCTGGAAGGATA+GGG 0.569303 1.1:-1083678 None:intergenic
CCCTGCTTACCAGTTGCACC+AGG 0.570056 1.1:-1084512 None:intergenic
CTGCAGGGCACAAAGGGCAA+TGG 0.570514 1.1:+1084569 MS.gene90654:CDS
GATTGAGCAGCAATAAATGA+GGG 0.573179 1.1:-1083522 None:intergenic
TCCAGCTCTGCAGGGCACAA+AGG 0.574271 1.1:+1084562 MS.gene90654:CDS
ATGGAATCAAATGCAAGTGT+CGG 0.577817 1.1:+1083369 MS.gene90654:CDS
TTAATCACATGCGGTGGTGG+TGG 0.579067 1.1:+1081092 MS.gene90654:CDS
AAGAAGAGACATTCCTTACC+TGG 0.579855 1.1:+1084494 MS.gene90654:CDS
TAGTAGAGAACAATTGCCTA+AGG 0.581315 1.1:+1084271 MS.gene90654:CDS
AAATGTGAAGCACACTTCTG+TGG 0.582438 1.1:+1082808 MS.gene90654:CDS
TCAGGATCAGCATTTCTGGA+AGG 0.588211 1.1:-1083684 None:intergenic
CCTGGTGCAACTGGTAAGCA+GGG 0.589383 1.1:+1084512 MS.gene90654:CDS
CTCGGTCTCTAGTTGGTCTG+AGG 0.591676 1.1:-1083460 None:intergenic
TAGAAGGATCACATTTGACA+TGG 0.592428 1.1:-1082984 None:intergenic
GCTCAGAAGTCACATTCAGA+TGG 0.597896 1.1:+1082559 MS.gene90654:CDS
AATCATGATTCAGGCTCAAG+AGG 0.598045 1.1:+1084248 MS.gene90654:CDS
AGAGGCTCCTCCCGAAAAGA+CGG 0.601939 1.1:+1083290 MS.gene90654:CDS
GATAATGACTGTTCGACACG+CGG 0.605175 1.1:-1084539 None:intergenic
CTGGATCAAGCAGTAGACGC+AGG 0.606218 1.1:+1084135 MS.gene90654:CDS
TGTTTAAGGTTAATCACATG+CGG 0.607247 1.1:+1081083 MS.gene90654:CDS
ATTATCACAGCAGATTCTGG+TGG 0.607983 1.1:-1082269 None:intergenic
ATGCCAAAGATTTAGTAGAA+GGG 0.611090 1.1:+1083781 MS.gene90654:CDS
AGTTGCAAGCTGCTGTTCGT+GGG 0.611911 1.1:+1082452 MS.gene90654:CDS
CTGAATCAACAACATTGCCA+CGG 0.613933 1.1:-1083908 None:intergenic
TGAATCAACAACATTGCCAC+GGG 0.613962 1.1:-1083907 None:intergenic
ATCTGGACTTGATCTTGGAG+AGG 0.618466 1.1:-1084447 None:intergenic
TTATTAGAGAAGATGAAGAG+AGG 0.625076 1.1:-1083254 None:intergenic
TGGTATCTCTGAGCAAACAT+CGG 0.629581 1.1:-1082204 None:intergenic
TCATTATCTCCAGCTCTGCA+GGG 0.630457 1.1:+1084554 MS.gene90654:CDS
GAAACTGTTAATGGCTCCCG+TGG 0.632617 1.1:+1083891 MS.gene90654:CDS
TATTCTCAACAAGCTTCACC+GGG 0.635415 1.1:+1084029 MS.gene90654:CDS
GTTCAAATTCAAATTCGGGT+AGG 0.637397 1.1:+1083562 MS.gene90654:CDS
GCAAGCTACACAATCTGCCA+AGG 0.638530 1.1:+1084406 MS.gene90654:CDS
AATTTGTACGACCGAACAGT+CGG 0.638857 1.1:+1083083 MS.gene90654:CDS
CGATTCACCTCTACCATCCG+AGG 0.641344 1.1:+1083179 MS.gene90654:CDS
CTCAGACCAACTAGAGACCG+AGG 0.645334 1.1:+1083461 MS.gene90654:CDS
GCTTGCTTGAAAGTGTGAGT+TGG 0.649171 1.1:-1083232 None:intergenic
TGGATCAAGCAGTAGACGCA+GGG 0.654626 1.1:+1084136 MS.gene90654:CDS
TCAAATTCGGGTAGGCCGAG+TGG 0.655550 1.1:+1083570 MS.gene90654:CDS
TCCTGGTCGAGCAAACCACT+CGG 0.660396 1.1:-1083585 None:intergenic
CACCGAAACTGAGAGTAAGG+TGG 0.667231 1.1:+1082238 MS.gene90654:CDS
TTAAGGTTAATCACATGCGG+TGG 0.672478 1.1:+1081086 MS.gene90654:CDS
TATCTCTGAGCAAACATCGG+AGG 0.674060 1.1:-1082201 None:intergenic
TCAGGCTCAAGAGTGTCTGT+CGG 0.675797 1.1:+1084203 MS.gene90654:CDS
AACAACATTAGGCTCAGCTG+AGG 0.679085 1.1:-1082132 None:intergenic
TCAGACCAACTAGAGACCGA+GGG 0.692845 1.1:+1083462 MS.gene90654:CDS
GATTCACCTCAGAAACAACA+TGG 0.720891 1.1:-1081130 None:intergenic
TGTGTAGCTTGCATGAAACG+GGG 0.721075 1.1:-1084395 None:intergenic
CAAACATCGGAGGATAACTG+TGG 0.737595 1.1:-1082191 None:intergenic
AAGGATTCTAATGATAAACG+CGG 0.747406 1.1:+1081990 MS.gene90654:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCACAAAAAAAAAAAAAAAA+AGG - chr1.1:1081439-1081458 None:intergenic 10.0%
!!! TTTTATTTTCTTTCTATTTT+TGG + chr1.1:1081316-1081335 MS.gene90654:intron 10.0%
!!! CTATTAAACGAATAAAATTT+TGG - chr1.1:1082654-1082673 None:intergenic 15.0%
!!! TTCATAATTGAATAGTTTTA+AGG + chr1.1:1081358-1081377 MS.gene90654:intron 15.0%
!! ACGAACAAAAGATTTATAAA+AGG - chr1.1:1082383-1082402 None:intergenic 20.0%
!! ATTTAGTATGAATGAACAAA+CGG - chr1.1:1082907-1082926 None:intergenic 20.0%
!! TTATGAATCAAAATCTTGTA+AGG + chr1.1:1082341-1082360 MS.gene90654:intron 20.0%
!! TTCTCTAATAAATGATAACT+TGG + chr1.1:1083266-1083285 MS.gene90654:CDS 20.0%
!! TTCTTTCCAAAATGAAATAA+TGG + chr1.1:1083350-1083369 MS.gene90654:CDS 20.0%
!!! TTATTTTTTTACTGTGGTTT+AGG + chr1.1:1082760-1082779 MS.gene90654:intron 20.0%
!!! TTGATTCCATTATTTCATTT+TGG - chr1.1:1083359-1083378 None:intergenic 20.0%
!!! TTTTATTCGTTTAATAGTTG+TGG + chr1.1:1082657-1082676 MS.gene90654:intron 20.0%
! AAATGATGACTCAAAAACTA+CGG + chr1.1:1082585-1082604 MS.gene90654:CDS 25.0%
! AATAAATGATAACTTGGAAG+AGG + chr1.1:1083272-1083291 MS.gene90654:CDS 25.0%
! AATAAGAAAAGAAACGAAGT+TGG - chr1.1:1081247-1081266 None:intergenic 25.0%
! AGAAAATTACATTTGGATCA+AGG + chr1.1:1083487-1083506 MS.gene90654:CDS 25.0%
! AGTTGAAGAAATAGATAGTA+CGG - chr1.1:1083718-1083737 None:intergenic 25.0%
! ATATACATCTTTATGCCAAT+GGG + chr1.1:1081669-1081688 MS.gene90654:intron 25.0%
! ATTGCTACTACTATTATCTT+TGG - chr1.1:1084366-1084385 None:intergenic 25.0%
! GATCAATTAGAAACTGTTAA+TGG + chr1.1:1083882-1083901 MS.gene90654:CDS 25.0%
! GATGTAAGTTGTACAATTTA+CGG + chr1.1:1081703-1081722 MS.gene90654:intron 25.0%
! TAAAGAAGTAAAATCAAGCA+AGG - chr1.1:1081525-1081544 None:intergenic 25.0%
! TAATTTACCTATCTCTGATT+CGG + chr1.1:1083860-1083879 MS.gene90654:CDS 25.0%
! TATATACATCTTTATGCCAA+TGG + chr1.1:1081668-1081687 MS.gene90654:intron 25.0%
! TATCAACTAAGCACAAAAAA+CGG - chr1.1:1081729-1081748 None:intergenic 25.0%
!! AAATGTAATTTTCTTTCCCT+CGG - chr1.1:1083481-1083500 None:intergenic 25.0%
!! TGAAATCTACTTCTTGTTTA+AGG + chr1.1:1081069-1081088 MS.gene90654:CDS 25.0%
!! TTTTGTTCGTGTTTCTTTTT+AGG + chr1.1:1082393-1082412 MS.gene90654:intron 25.0%
!! TTTTTCTTTAATGATGTTCC+AGG + chr1.1:1081965-1081984 MS.gene90654:intron 25.0%
!!! GTCGTTTTATTTTTTTACTG+TGG + chr1.1:1082754-1082773 MS.gene90654:intron 25.0%
AACTGAAGAATAACTTGAGA+TGG - chr1.1:1083156-1083175 None:intergenic 30.0%
AAGGATTCTAATGATAAACG+CGG + chr1.1:1081990-1082009 MS.gene90654:CDS 30.0%
AGAGGAATTTGCATTTATCT+TGG - chr1.1:1084432-1084451 None:intergenic 30.0%
ATGCCAAAGATTTAGTAGAA+GGG + chr1.1:1083781-1083800 MS.gene90654:CDS 30.0%
CAAATCTTCTCAACAACATT+AGG - chr1.1:1082146-1082165 None:intergenic 30.0%
CTACTACTATTATCTTTGGT+AGG - chr1.1:1084362-1084381 None:intergenic 30.0%
CTGAGTTCAAATTCAAATTC+GGG + chr1.1:1083558-1083577 MS.gene90654:CDS 30.0%
CTGTTTGGATAACATTGAAT+AGG + chr1.1:1081383-1081402 MS.gene90654:intron 30.0%
GAGAATAAAATCAACAAGAC+TGG + chr1.1:1084116-1084135 MS.gene90654:CDS 30.0%
GAGATAGGTAAATTAGAAGT+CGG - chr1.1:1083855-1083874 None:intergenic 30.0%
GAGGATTGAAAACTAATACT+TGG - chr1.1:1082116-1082135 None:intergenic 30.0%
GAGGGAAAGAAAATTACATT+TGG + chr1.1:1083480-1083499 MS.gene90654:CDS 30.0%
GCTAATTCAAATCAAGATTC+AGG + chr1.1:1084185-1084204 MS.gene90654:CDS 30.0%
GCTGAGTTCAAATTCAAATT+CGG + chr1.1:1083557-1083576 MS.gene90654:CDS 30.0%
TATCCCTTCTACTAAATCTT+TGG - chr1.1:1083787-1083806 None:intergenic 30.0%
TTATTAGAGAAGATGAAGAG+AGG - chr1.1:1083257-1083276 None:intergenic 30.0%
TTGGAAAGAATCAATTTCAG+TGG - chr1.1:1083340-1083359 None:intergenic 30.0%
TTGTGAAAAACACTAAACCT+CGG - chr1.1:1081172-1081191 None:intergenic 30.0%
! ATCATATGTAACCGTCTTTT+CGG - chr1.1:1083304-1083323 None:intergenic 30.0%
! GAATCTTGATTTGAATTAGC+AGG - chr1.1:1084185-1084204 None:intergenic 30.0%
! TGTTTAAGGTTAATCACATG+CGG + chr1.1:1081083-1081102 MS.gene90654:CDS 30.0%
! TTTGGTATGTTTCTTTTCTG+AGG + chr1.1:1082316-1082335 MS.gene90654:intron 30.0%
!! TGAAATTTTGATCGTGATTC+AGG + chr1.1:1081902-1081921 MS.gene90654:intron 30.0%
!!! AATAGTTTTAAGGTGCTGTT+TGG + chr1.1:1081368-1081387 MS.gene90654:intron 30.0%
!!! ACATGGATAGGTTTGTTTTT+TGG - chr1.1:1082970-1082989 None:intergenic 30.0%
AATATCCTCTAATTGAAGCC+TGG - chr1.1:1082298-1082317 None:intergenic 35.0%
AATTGATCCGAATCAGAGAT+AGG - chr1.1:1083870-1083889 None:intergenic 35.0%
ATGACAATTCCTGAATCTCA+AGG + chr1.1:1084059-1084078 MS.gene90654:CDS 35.0%
ATGATTATCACAGCAGATTC+TGG - chr1.1:1082275-1082294 None:intergenic 35.0%
ATGGAATCAAATGCAAGTGT+CGG + chr1.1:1083369-1083388 MS.gene90654:CDS 35.0%
ATTCTAATGATAAACGCGGT+TGG + chr1.1:1081994-1082013 MS.gene90654:CDS 35.0%
ATTTGCATTTATCTTGGCCT+TGG - chr1.1:1084426-1084445 None:intergenic 35.0%
CCTGAAAACAAGATTGATCA+TGG + chr1.1:1083813-1083832 MS.gene90654:CDS 35.0%
GAAAGAATCAATTTCAGTGG+AGG - chr1.1:1083337-1083356 None:intergenic 35.0%
GATGCCAAAGATTTAGTAGA+AGG + chr1.1:1083780-1083799 MS.gene90654:CDS 35.0%
GATTGAGCAGCAATAAATGA+GGG - chr1.1:1083525-1083544 None:intergenic 35.0%
GGCTTCAATTAGAGGATATT+TGG + chr1.1:1082298-1082317 MS.gene90654:CDS 35.0%
GTCAAATGTGATCCTTCTAA+AGG + chr1.1:1082988-1083007 MS.gene90654:CDS 35.0%
GTTCAAATTCAAATTCGGGT+AGG + chr1.1:1083562-1083581 MS.gene90654:CDS 35.0%
TAGAAGGATCACATTTGACA+TGG - chr1.1:1082987-1083006 None:intergenic 35.0%
TAGTAGAGAACAATTGCCTA+AGG + chr1.1:1084271-1084290 MS.gene90654:CDS 35.0%
TATCATTAGAATCCTTGACC+TGG - chr1.1:1081986-1082005 None:intergenic 35.0%
TCATCACTTTCTTGATTCTG+AGG - chr1.1:1083411-1083430 None:intergenic 35.0%
TGATTGAGCAGCAATAAATG+AGG - chr1.1:1083526-1083545 None:intergenic 35.0%
TTTAATGATGTTCCAGGTCA+AGG + chr1.1:1081971-1081990 MS.gene90654:intron 35.0%
! AAGTCAGATGCAATTTTCTC+AGG - chr1.1:1083969-1083988 None:intergenic 35.0%
! ATTGTGTAGCTTGCATGAAA+CGG - chr1.1:1084400-1084419 None:intergenic 35.0%
! TCATATGTAACCGTCTTTTC+GGG - chr1.1:1083303-1083322 None:intergenic 35.0%
! TCTTGATTCTGAGGAGAATT+AGG - chr1.1:1083402-1083421 None:intergenic 35.0%
!! TGAGTTGGTATCATAGTAAG+TGG - chr1.1:1083220-1083239 None:intergenic 35.0%
!! TTCGTGTTTCTTTTTAGGCT+CGG + chr1.1:1082398-1082417 MS.gene90654:intron 35.0%
!!! CCATGATCAATCTTGTTTTC+AGG - chr1.1:1083816-1083835 None:intergenic 35.0%
AAAACGACACACTTAGAGCA+AGG - chr1.1:1082742-1082761 None:intergenic 40.0%
AAACTTGATCTCGTGGATCT+AGG - chr1.1:1081780-1081799 None:intergenic 40.0%
AAATGTGAAGCACACTTCTG+TGG + chr1.1:1082808-1082827 MS.gene90654:CDS 40.0%
AACACCTTCAAGTCAGGTTT+CGG + chr1.1:1084082-1084101 MS.gene90654:CDS 40.0%
AAGAAGAGACATTCCTTACC+TGG + chr1.1:1084494-1084513 MS.gene90654:CDS 40.0%
AAGGTGATTCTGCTTGGAAA+TGG + chr1.1:1083007-1083026 MS.gene90654:CDS 40.0%
AATCATGATTCAGGCTCAAG+AGG + chr1.1:1084248-1084267 MS.gene90654:CDS 40.0%
AATTTGTACGACCGAACAGT+CGG + chr1.1:1083083-1083102 MS.gene90654:CDS 40.0%
ACAAACGGATGACGTTAAAC+AGG - chr1.1:1082892-1082911 None:intergenic 40.0%
ACTTTCAGGATCAGCATTTC+TGG - chr1.1:1083691-1083710 None:intergenic 40.0%
AGAAATGCTGATCCTGAAAG+TGG + chr1.1:1083690-1083709 MS.gene90654:CDS 40.0%
AGATGCATGAGTGCTATCTT+TGG - chr1.1:1083115-1083134 None:intergenic 40.0%
ATCATCCAGGCTTCAATTAG+AGG + chr1.1:1082290-1082309 MS.gene90654:CDS 40.0%
ATCCACCTTACTCTCAGTTT+CGG - chr1.1:1082243-1082262 None:intergenic 40.0%
ATCTGCTGTGATAATCATCC+AGG + chr1.1:1082277-1082296 MS.gene90654:CDS 40.0%
ATGCACAAAACTTGATCTCG+TGG - chr1.1:1081787-1081806 None:intergenic 40.0%
ATTATCACAGCAGATTCTGG+TGG - chr1.1:1082272-1082291 None:intergenic 40.0%
ATTCCAACAGCTTCAATGGA+AGG - chr1.1:1082942-1082961 None:intergenic 40.0%
CAAGCAGAATCACCTTTAGA+AGG - chr1.1:1083003-1083022 None:intergenic 40.0%
CTCTCTCTTAGATAATTCCG+AGG + chr1.1:1081152-1081171 MS.gene90654:intron 40.0%
CTGAATCAACAACATTGCCA+CGG - chr1.1:1083911-1083930 None:intergenic 40.0%
GACCACATCTTCAGCAAATA+AGG + chr1.1:1082076-1082095 MS.gene90654:CDS 40.0%
GATTCACCTCAGAAACAACA+TGG - chr1.1:1081133-1081152 None:intergenic 40.0%
GCGAATGCAAATCATGATTC+AGG + chr1.1:1084239-1084258 MS.gene90654:CDS 40.0%
GGAAAGAGACGCAATTCATT+TGG + chr1.1:1084305-1084324 MS.gene90654:CDS 40.0%
GGAATTGTCATATAGCTTCC+CGG - chr1.1:1084050-1084069 None:intergenic 40.0%
GGAGAGATGGATGTTTGTTT+CGG + chr1.1:1083032-1083051 MS.gene90654:CDS 40.0%
GGATCACATTTGACATGGAT+AGG - chr1.1:1082982-1083001 None:intergenic 40.0%
GTATTCTCAACAAGCTTCAC+CGG + chr1.1:1084028-1084047 MS.gene90654:CDS 40.0%
GTTTCGGTGACAATTGTTTC+TGG - chr1.1:1082227-1082246 None:intergenic 40.0%
TAGCACTCATGCATCTCAAT+CGG + chr1.1:1083119-1083138 MS.gene90654:CDS 40.0%
TATTCTCAACAAGCTTCACC+GGG + chr1.1:1084029-1084048 MS.gene90654:CDS 40.0%
TGAATCAACAACATTGCCAC+GGG - chr1.1:1083910-1083929 None:intergenic 40.0%
TGATTCTGCTTGGAAATGGT+TGG + chr1.1:1083011-1083030 MS.gene90654:CDS 40.0%
TGGTATCTCTGAGCAAACAT+CGG - chr1.1:1082207-1082226 None:intergenic 40.0%
TTAAGGTTAATCACATGCGG+TGG + chr1.1:1081086-1081105 MS.gene90654:CDS 40.0%
TTGGTAGGTTCTTGATCAGT+GGG - chr1.1:1084347-1084366 None:intergenic 40.0%
TTGGTTGCATGAAACATCCT+CGG - chr1.1:1081228-1081247 None:intergenic 40.0%
TTTCCATTCAGCTGATCCTT+AGG - chr1.1:1084290-1084309 None:intergenic 40.0%
TTTCCTTCCATTGAAGCTGT+TGG + chr1.1:1082936-1082955 MS.gene90654:intron 40.0%
TTTGGTAGGTTCTTGATCAG+TGG - chr1.1:1084348-1084367 None:intergenic 40.0%
! ATAAACGCGGTTGGAGTTTT+CGG + chr1.1:1082003-1082022 MS.gene90654:CDS 40.0%
! ATCAGCATTTCTGGAAGGAT+AGG - chr1.1:1083682-1083701 None:intergenic 40.0%
! CAGCAATAAGTTTGCTTCTC+AGG + chr1.1:1082841-1082860 MS.gene90654:CDS 40.0%
! CTCCTTATTTGCTGAAGATG+TGG - chr1.1:1082081-1082100 None:intergenic 40.0%
! CTTCTAAAGGTGATTCTGCT+TGG + chr1.1:1083001-1083020 MS.gene90654:CDS 40.0%
! GAATCATGATTTGCATTCGC+AGG - chr1.1:1084239-1084258 None:intergenic 40.0%
! GTTGATTCCAACAGCTTCAA+TGG - chr1.1:1082946-1082965 None:intergenic 40.0%
! TCAGCATTTCTGGAAGGATA+GGG - chr1.1:1083681-1083700 None:intergenic 40.0%
! TTGTGTAGCTTGCATGAAAC+GGG - chr1.1:1084399-1084418 None:intergenic 40.0%
!! AGATCACCATGTTGTTTCTG+AGG + chr1.1:1081124-1081143 MS.gene90654:CDS 40.0%
!! GTGTGTTTTTGTTTGATCCG+AGG + chr1.1:1081208-1081227 MS.gene90654:intron 40.0%
AACAACATTAGGCTCAGCTG+AGG - chr1.1:1082135-1082154 None:intergenic 45.0%
ACAAACTGAGACTGTTCTGC+AGG + chr1.1:1083998-1084017 MS.gene90654:CDS 45.0%
ACCTGACAGATCAGAGACTT+TGG + chr1.1:1083746-1083765 MS.gene90654:CDS 45.0%
ATCTGGACTTGATCTTGGAG+AGG - chr1.1:1084450-1084469 None:intergenic 45.0%
ATGTCAGTGTCATGCACATC+TGG - chr1.1:1084467-1084486 None:intergenic 45.0%
ATTTGCATTCGCAGGAGACT+TGG - chr1.1:1084231-1084250 None:intergenic 45.0%
CAAACATCGGAGGATAACTG+TGG - chr1.1:1082194-1082213 None:intergenic 45.0%
CATCAAAAGTCACAGCCCAT+TGG - chr1.1:1081687-1081706 None:intergenic 45.0%
CTTGGAAATGGTTGGAGAGA+TGG + chr1.1:1083019-1083038 MS.gene90654:CDS 45.0%
GACTGTTCGGTCGTACAAAT+TGG - chr1.1:1083084-1083103 None:intergenic 45.0%
GATAATGACTGTTCGACACG+CGG - chr1.1:1084542-1084561 None:intergenic 45.0%
GATAGTACGGTACCACTTTC+AGG - chr1.1:1083705-1083724 None:intergenic 45.0%
GCTCAGAAGTCACATTCAGA+TGG + chr1.1:1082559-1082578 MS.gene90654:CDS 45.0%
TATCTCTGAGCAAACATCGG+AGG - chr1.1:1082204-1082223 None:intergenic 45.0%
TCAAGGAACACCTTCAAGTC+AGG + chr1.1:1084076-1084095 MS.gene90654:CDS 45.0%
TCATTATCTCCAGCTCTGCA+GGG + chr1.1:1084554-1084573 MS.gene90654:CDS 45.0%
TCCAAAGTCTCTGATCTGTC+AGG - chr1.1:1083750-1083769 None:intergenic 45.0%
TGCACATCTGGACTTGATCT+TGG - chr1.1:1084455-1084474 None:intergenic 45.0%
TGTCACCGAAACTGAGAGTA+AGG + chr1.1:1082235-1082254 MS.gene90654:CDS 45.0%
TTGCCTAAGGATCAGCTGAA+TGG + chr1.1:1084284-1084303 MS.gene90654:CDS 45.0%
TTTGCTCGACCAGGATGTAT+CGG + chr1.1:1083593-1083612 MS.gene90654:CDS 45.0%
! GCTTGCTTGAAAGTGTGAGT+TGG - chr1.1:1083235-1083254 None:intergenic 45.0%
! GTTTTCGGAAGAGATCTGCA+AGG + chr1.1:1082018-1082037 MS.gene90654:CDS 45.0%
! TCAGGATCAGCATTTCTGGA+AGG - chr1.1:1083687-1083706 None:intergenic 45.0%
! TCGTACAAGGAGCTGCATTT+TGG - chr1.1:1082530-1082549 None:intergenic 45.0%
! TGTGTAGCTTGCATGAAACG+GGG - chr1.1:1084398-1084417 None:intergenic 45.0%
! TTCACCGAAACCTGACTTGA+AGG - chr1.1:1084089-1084108 None:intergenic 45.0%
! TTGGTTAGAAGGCATGCTGT+TGG + chr1.1:1082478-1082497 MS.gene90654:CDS 45.0%
! TTTGGTTTCGTCCGACTGTT+CGG - chr1.1:1083097-1083116 None:intergenic 45.0%
!! GAAGGTGTTCCTTGAGATTC+AGG - chr1.1:1084071-1084090 None:intergenic 45.0%
!! TATGTAACCGTCTTTTCGGG+AGG - chr1.1:1083300-1083319 None:intergenic 45.0%
AAGTTGCAAGCTGCTGTTCG+TGG + chr1.1:1082451-1082470 MS.gene90654:CDS 50.0%
AGGCTCAACAGCATGCTCTT+TGG - chr1.1:1083949-1083968 None:intergenic 50.0%
AGGTTAATCACATGCGGTGG+TGG + chr1.1:1081089-1081108 MS.gene90654:CDS 50.0%
AGTTGCAAGCTGCTGTTCGT+GGG + chr1.1:1082452-1082471 MS.gene90654:CDS 50.0%
CACCGAAACTGAGAGTAAGG+TGG + chr1.1:1082238-1082257 MS.gene90654:CDS 50.0%
CATTCCTTACCTGGTGCAAC+TGG + chr1.1:1084503-1084522 MS.gene90654:CDS 50.0%
CTCGGATGGTAGAGGTGAAT+CGG - chr1.1:1083181-1083200 None:intergenic 50.0%
CTTACCAGTTGCACCAGGTA+AGG - chr1.1:1084510-1084529 None:intergenic 50.0%
GAAACTGTTAATGGCTCCCG+TGG + chr1.1:1083891-1083910 MS.gene90654:CDS 50.0%
GCAAGCTACACAATCTGCCA+AGG + chr1.1:1084406-1084425 MS.gene90654:CDS 50.0%
GGCAGTCTTCTCTTCATCCT+CGG - chr1.1:1083199-1083218 None:intergenic 50.0%
GTCATTATCTCCAGCTCTGC+AGG + chr1.1:1084553-1084572 MS.gene90654:CDS 50.0%
GTCTTCTCTTCATCCTCGGA+TGG - chr1.1:1083195-1083214 None:intergenic 50.0%
GTGACTCAACCGATACATCC+TGG - chr1.1:1083605-1083624 None:intergenic 50.0%
TCAGACCAACTAGAGACCGA+GGG + chr1.1:1083462-1083481 MS.gene90654:CDS 50.0%
TCTTCATCCTCGGATGGTAG+AGG - chr1.1:1083189-1083208 None:intergenic 50.0%
TCTTTCCCTCGGTCTCTAGT+TGG - chr1.1:1083470-1083489 None:intergenic 50.0%
TGATCTCGTGGATCTAGGCT+CGG - chr1.1:1081775-1081794 None:intergenic 50.0%
TGGATCAAGCAGTAGACGCA+GGG + chr1.1:1084136-1084155 MS.gene90654:CDS 50.0%
TTCGTGGGCACTTGGTTAGA+AGG + chr1.1:1082467-1082486 MS.gene90654:CDS 50.0%
! GGTTCTTGATCAGTGGGTTC+AGG - chr1.1:1084341-1084360 None:intergenic 50.0%
! TTAATCACATGCGGTGGTGG+TGG + chr1.1:1081092-1081111 MS.gene90654:CDS 50.0%
!! GCATGCTCTTTGGAGTTCTC+AGG - chr1.1:1083939-1083958 None:intergenic 50.0%
!! TCAGGCTCAAGAGTGTCTGT+CGG + chr1.1:1084203-1084222 MS.gene90654:CDS 50.0%
ACCTGGTGCAACTGGTAAGC+AGG + chr1.1:1084511-1084530 MS.gene90654:CDS 55.0%
AGAGGCTCCTCCCGAAAAGA+CGG + chr1.1:1083290-1083309 MS.gene90654:CDS 55.0%
CCTGGTGCAACTGGTAAGCA+GGG + chr1.1:1084512-1084531 MS.gene90654:CDS 55.0%
CGATTCACCTCTACCATCCG+AGG + chr1.1:1083179-1083198 MS.gene90654:CDS 55.0%
CTCAGACCAACTAGAGACCG+AGG + chr1.1:1083461-1083480 MS.gene90654:CDS 55.0%
CTGGATCAAGCAGTAGACGC+AGG + chr1.1:1084135-1084154 MS.gene90654:CDS 55.0%
GAGCATGTCGTGCTCGTACA+AGG - chr1.1:1082543-1082562 None:intergenic 55.0%
TCAAATTCGGGTAGGCCGAG+TGG + chr1.1:1083570-1083589 MS.gene90654:CDS 55.0%
TCCTGGTCGAGCAAACCACT+CGG - chr1.1:1083588-1083607 None:intergenic 55.0%
! CTCGGTCTCTAGTTGGTCTG+AGG - chr1.1:1083463-1083482 None:intergenic 55.0%
AGCTGCTGTTCGTGGGCACT+TGG + chr1.1:1082459-1082478 MS.gene90654:CDS 60.0%
CCAGCTCTGCAGGGCACAAA+GGG + chr1.1:1084563-1084582 MS.gene90654:CDS 60.0%
CCCTGCTTACCAGTTGCACC+AGG - chr1.1:1084515-1084534 None:intergenic 60.0%
CTGCAGGGCACAAAGGGCAA+TGG + chr1.1:1084569-1084588 MS.gene90654:CDS 60.0%
TCCAGCTCTGCAGGGCACAA+AGG + chr1.1:1084562-1084581 MS.gene90654:CDS 60.0%
TCGTGGATCTAGGCTCGGCA+TGG - chr1.1:1081770-1081789 None:intergenic 60.0%
!! AGACGCAGGGTACTGTCTGC+TGG + chr1.1:1084149-1084168 MS.gene90654:CDS 60.0%
CCCTTTGTGCCCTGCAGAGC+TGG - chr1.1:1084566-1084585 None:intergenic 65.0%
!! GCCGAGTGGTTTGCTCGACC+AGG + chr1.1:1083584-1083603 MS.gene90654:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 1081065 1084599 1081065 ID=MS.gene90654
chr1.1 mRNA 1081065 1084599 1081065 ID=MS.gene90654.t1;Parent=MS.gene90654
chr1.1 exon 1081065 1081145 1081065 ID=MS.gene90654.t1.exon1;Parent=MS.gene90654.t1
chr1.1 CDS 1081065 1081145 1081065 ID=cds.MS.gene90654.t1;Parent=MS.gene90654.t1
chr1.1 exon 1081987 1082319 1081987 ID=MS.gene90654.t1.exon2;Parent=MS.gene90654.t1
chr1.1 CDS 1081987 1082319 1081987 ID=cds.MS.gene90654.t1;Parent=MS.gene90654.t1
chr1.1 exon 1082415 1082606 1082415 ID=MS.gene90654.t1.exon3;Parent=MS.gene90654.t1
chr1.1 CDS 1082415 1082606 1082415 ID=cds.MS.gene90654.t1;Parent=MS.gene90654.t1
chr1.1 exon 1082782 1082862 1082782 ID=MS.gene90654.t1.exon4;Parent=MS.gene90654.t1
chr1.1 CDS 1082782 1082862 1082782 ID=cds.MS.gene90654.t1;Parent=MS.gene90654.t1
chr1.1 exon 1082952 1084599 1082952 ID=MS.gene90654.t1.exon5;Parent=MS.gene90654.t1
chr1.1 CDS 1082952 1084599 1082952 ID=cds.MS.gene90654.t1;Parent=MS.gene90654.t1
Gene Sequence

>MS.gene90654

ATGGTGAAATCTACTTCTTGTTTAAGGTTAATCACATGCGGTGGTGGTGGTGACAAAGAAGATCACCATGTTGTTTCTGAGGTGAATCTCTCTCTTAGATAATTCCGAGGTTTAGTGTTTTTCACAATTTTTCAGTTTTATGTGTGTGTTTTTGTTTGATCCGAGGATGTTTCATGCAACCAACTTCGTTTCTTTTCTTATTATCACGTTTCTTGCATTCATTAATTACTTAATTAATTTGCTGTTTAATGTTTTATTTTCTTTCTATTTTTGGATTTATATTCAAGATTTCGTTCATAATTGAATAGTTTTAAGGTGCTGTTTGGATAACATTGAATAGGAAAATTCATGTTTTTTTTTTTTTTGAATTTCCTTTTTTTTTTTTTTTTTGTGATCTTTGATCTCAAGATTAGTAATGAATATCATGTAGTTGATGATGCTCTCTGAAAATCATTTTCCTTGCTTGATTTTACTTCTTTACTAATTTTTCTTGTTTATTTTGATAATCACTAGTATTTCACGATGCTAGAAAATCTGATTATTGTGAATTAATTAGTATTTTTCACATAATAATTGAAGTAAATTAAATTCATGAGTGTGAATTATATACATCTTTATGCCAATGGGCTGTGACTTTTGATGTAAGTTGTACAATTTACGGCCGTTTTTTGTGCTTAGTTGATATGAACTTAGTTATTTGAACCATGCCGAGCCTAGATCCACGAGATCAAGTTTTGTGCATAAAACTTAGTTATAAACATCTAGATAAAGTTTGTGCATCGTATAAGTAAAATGAGATAGTTGCACATTGTACTGTAGTAAGTTTTATTGAATAAGTGAAATTTTGATCGTGATTCAGGAAATATATATTTATGCATTCAAGTTTGTTCTCACAATTGATTTTTCTTTAATGATGTTCCAGGTCAAGGATTCTAATGATAAACGCGGTTGGAGTTTTCGGAAGAGATCTGCAAGGCATCGTGTGCTTAGCAATACTGTGATAACAACAGAGACCACATCTTCAGCAAATAAGGAGACTTCAGAATATCCAAGTATTAGTTTTCAATCCTCAGCTGAGCCTAATGTTGTTGAGAAGATTTGTACGACAGATTTCTCTAATGAGAAGCCACAGTTATCCTCCGATGTTTGCTCAGAGATACCAGAAACAATTGTCACCGAAACTGAGAGTAAGGTGGATGTCAATCCACCAGAATCTGCTGTGATAATCATCCAGGCTTCAATTAGAGGATATTTGGTATGTTTCTTTTCTGAGGTTTTATGAATCAAAATCTTGTAAGGAGAAAACGCTCATTTTCCTTTTATAAATCTTTTGTTCGTGTTTCTTTTTAGGCTCGGAGAGCACTTCTAAAGAGTAAGAATGTAGTGAAGTTGCAAGCTGCTGTTCGTGGGCACTTGGTTAGAAGGCATGCTGTTGGAACATTGCGATGTGTTCAAGCTATTGCCAAAATGCAGCTCCTTGTACGAGCACGACATGCTCAGAAGTCACATTCAGATGGAAAAAATGATGACTCAAAAACTACGGTATATCTATTTGAATTTACTTCGTAGTTACTAATTTCATGATACCAAAATTTTATTCGTTTAATAGTTGTGGTTTCATTGAAGAGATATGTTATTACATTTACGTTACTATCAGATGATATGTGCTGCAAGCCTTGCTCTAAGTGTGTCGTTTTATTTTTTTACTGTGGTTTAGGATAATGAGCATTACACTGCTGAATCAAATGTGAAGCACACTTCTGTGGAAAAGCTTCTCAGCAATAAGTTTGCTTCTCAGGTACAATTAACATATTGTTTGTTTTGCCTGTTTAACGTCATCCGTTTGTTCATTCATACTAAATCTTCTGTTGTTTCCTTCCATTGAAGCTGTTGGAATCAACACCAAAAAACAAACCTATCCATGTCAAATGTGATCCTTCTAAAGGTGATTCTGCTTGGAAATGGTTGGAGAGATGGATGTTTGTTTCGGCAAAAGATTCTGCAGAAGATAAAAAGCCAATTTGTACGACCGAACAGTCGGACGAAACCAAAGATAGCACTCATGCATCTCAATCGGAAACAGATATTCCATCTCAAGTTATTCTTCAGTTAGCCGATTCACCTCTACCATCCGAGGATGAAGAGAAGACTGCCACTTACTATGATACCAACTCACACTTTCAAGCAAGCCTCTCTTCATCTTCTCTAATAAATGATAACTTGGAAGAGGCTCCTCCCGAAAAGACGGTTACATATGATTCTAAAGTAACCTCCACTGAAATTGATTCTTTCCAAAATGAAATAATGGAATCAAATGCAAGTGTCGGACATGAGCCTAATTCTCCTCAGAATCAAGAAAGTGATGAACAGTACAAACAATCAACGAAAGCATTTGCCTCAGACCAACTAGAGACCGAGGGAAAGAAAATTACATTTGGATCAAGGAAGTTAAGCAATCCCTCATTTATTGCTGCTCAATCAAAATTTGAAGAGCTGAGTTCAAATTCAAATTCGGGTAGGCCGAGTGGTTTGCTCGACCAGGATGTATCGGTTGAGTCACAAGCAGATAGTGCATATATATCAAAAGAGTTCATTTCATCTGAGAATTCTATGCCCTATCCTTCCAGAAATGCTGATCCTGAAAGTGGTACCGTACTATCTATTTCTTCAACTCTTGATTCACCTGACAGATCAGAGACTTTGGAAATCGAGCACGATGCCAAAGATTTAGTAGAAGGGATAGTTAATCCTGAAAACAAGATTGATCATGGTGTTGAAGCAAATACTCCGACTTCTAATTTACCTATCTCTGATTCGGATCAATTAGAAACTGTTAATGGCTCCCGTGGCAATGTTGTTGATTCAGTGATGCCTGAGAACTCCAAAGAGCATGCTGTTGAGCCTGAGAAAATTGCATCTGACTTGTTTAGAGAACAAACTGAGACTGTTCTGCAGGATTTCACGTATTCTCAACAAGCTTCACCGGGAAGCTATATGACAATTCCTGAATCTCAAGGAACACCTTCAAGTCAGGTTTCGGTGAAACATAAAGAGAATAAAATCAACAAGACTGGATCAAGCAGTAGACGCAGGGTACTGTCTGCTGGTAACAAGTCTCCTGCTAATTCAAATCAAGATTCAGGCTCAAGAGTGTCTGTCGGTACCAAGTCTCCTGCGAATGCAAATCATGATTCAGGCTCAAGAGGTAGTAGAGAACAATTGCCTAAGGATCAGCTGAATGGAAAGAGACGCAATTCATTTGGCTCAATAAAACCTGAACCCACTGATCAAGAACCTACCAAAGATAATAGTAGTAGCAATAATACTCTTCCCCGTTTCATGCAAGCTACACAATCTGCCAAGGCCAAGATAAATGCAAATTCCTCTCCAAGATCAAGTCCAGATGTGCATGACACTGACATTAATATCAAGAAGAGACATTCCTTACCTGGTGCAACTGGTAAGCAGGGTTCTCCGCGTGTCGAACAGTCATTATCTCCAGCTCTGCAGGGCACAAAGGGCAATGGTTCATCTG
Protein sequence

>MS.gene90654.t1

MVKSTSCLRLITCGGGGDKEDHHVVSEVKDSNDKRGWSFRKRSARHRVLSNTVITTETTSSANKETSEYPSISFQSSAEPNVVEKICTTDFSNEKPQLSSDVCSEIPETIVTETESKVDVNPPESAVIIIQASIRGYLARRALLKSKNVVKLQAAVRGHLVRRHAVGTLRCVQAIAKMQLLVRARHAQKSHSDGKNDDSKTTDNEHYTAESNVKHTSVEKLLSNKFASQLLESTPKNKPIHVKCDPSKGDSAWKWLERWMFVSAKDSAEDKKPICTTEQSDETKDSTHASQSETDIPSQVILQLADSPLPSEDEEKTATYYDTNSHFQASLSSSSLINDNLEEAPPEKTVTYDSKVTSTEIDSFQNEIMESNASVGHEPNSPQNQESDEQYKQSTKAFASDQLETEGKKITFGSRKLSNPSFIAAQSKFEELSSNSNSGRPSGLLDQDVSVESQADSAYISKEFISSENSMPYPSRNADPESGTVLSISSTLDSPDRSETLEIEHDAKDLVEGIVNPENKIDHGVEANTPTSNLPISDSDQLETVNGSRGNVVDSVMPENSKEHAVEPEKIASDLFREQTETVLQDFTYSQQASPGSYMTIPESQGTPSSQVSVKHKENKINKTGSSSRRRVLSAGNKSPANSNQDSGSRVSVGTKSPANANHDSGSRGSREQLPKDQLNGKRRNSFGSIKPEPTDQEPTKDNSSSNNTLPRFMQATQSAKAKINANSSPRSSPDVHDTDINIKKRHSLPGATGKQGSPRVEQSLSPALQGTKGNGSS