Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90695.t1 | XP_013466127.1 | 89.5 | 143 | 13 | 1 | 1 | 141 | 1 | 143 | 6.40E-66 | 260 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90695.t1 | I3SQ79 | 89.5 | 143 | 13 | 1 | 1 | 141 | 1 | 143 | 4.6e-66 | 260.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057086 | MS.gene90695 | -0.826919 | 2.08E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90695.t1 | MTR_1g022395 | 89.510 | 143 | 13 | 1 | 1 | 141 | 1 | 143 | 7.04e-92 | 262 |
| MS.gene90695.t1 | MTR_3g111970 | 62.937 | 143 | 44 | 5 | 1 | 140 | 1 | 137 | 4.01e-55 | 169 |
| MS.gene90695.t1 | MTR_3g111970 | 63.636 | 121 | 35 | 5 | 1 | 118 | 1 | 115 | 2.08e-44 | 141 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90695.t1 | AT1G19530 | 41.538 | 130 | 57 | 3 | 1 | 129 | 1 | 112 | 2.95e-18 | 75.5 |
| MS.gene90695.t1 | AT1G19530 | 41.538 | 130 | 57 | 3 | 1 | 129 | 1 | 112 | 2.95e-18 | 75.5 |
Find 35 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGATGAAATTGATGCTTAT+TGG | 0.156482 | 1.4:-1877864 | MS.gene90695:CDS |
| AATCATATGGTACCTGATTT+AGG | 0.175839 | 1.4:+1878045 | None:intergenic |
| GAACGTACTTGACACAAATT+TGG | 0.215908 | 1.4:-1876719 | MS.gene90695:CDS |
| GTCAAGTACGTTCATATTAA+AGG | 0.224800 | 1.4:+1876729 | None:intergenic |
| CTGAATAGTCTCTGACAAAT+TGG | 0.254276 | 1.4:+1877798 | None:intergenic |
| CTGTCTCAGCCATACTTAAA+AGG | 0.269763 | 1.4:-1877903 | MS.gene90695:intron |
| CAGTTTCATGTTGCCAGTTT+GGG | 0.286936 | 1.4:-1876478 | MS.gene90695:CDS |
| CAATTTGTCAGAGACTATTC+AGG | 0.322635 | 1.4:-1877797 | MS.gene90695:intron |
| ACAGTTTCATGTTGCCAGTT+TGG | 0.323503 | 1.4:-1876479 | MS.gene90695:CDS |
| GCAGGTGGACTAAGAGTAAC+TGG | 0.334701 | 1.4:-1876555 | MS.gene90695:intron |
| TCAAGTACGTTCATATTAAA+GGG | 0.359264 | 1.4:+1876730 | None:intergenic |
| TCTTCAATTGATCCTAAATC+AGG | 0.362673 | 1.4:-1878057 | MS.gene90695:intron |
| TTGGATGCAGCTTCCATCAT+TGG | 0.363993 | 1.4:+1876514 | None:intergenic |
| GGGTTCTCTTATGGCAGGAT+GGG | 0.445491 | 1.4:-1878085 | MS.gene90695:CDS |
| TCTGAATGAGCCACCAATGA+TGG | 0.467236 | 1.4:-1876527 | MS.gene90695:CDS |
| CCATGCCCGTGGCCCACGTA+AGG | 0.471373 | 1.4:-1876756 | MS.gene90695:CDS |
| AAACTGTGCTGCATACTTGT+TGG | 0.471706 | 1.4:+1876495 | None:intergenic |
| GGGTCCCTTACGTGGGCCAC+GGG | 0.472849 | 1.4:+1876751 | None:intergenic |
| GGGGTCCCTTACGTGGGCCA+CGG | 0.474803 | 1.4:+1876750 | None:intergenic |
| CCTTACGTGGGCCACGGGCA+TGG | 0.492032 | 1.4:+1876756 | None:intergenic |
| GGTTCTCTTATGGCAGGATG+GGG | 0.492223 | 1.4:-1878084 | MS.gene90695:CDS |
| CAATCACTAACTTCATCTAC+GGG | 0.495529 | 1.4:+1876439 | None:intergenic |
| GAAATCAAAGAAGAAGATAG+AGG | 0.499005 | 1.4:-1877842 | MS.gene90695:CDS |
| GATGCAGCTTCCATCATTGG+TGG | 0.505474 | 1.4:+1876517 | None:intergenic |
| TGGGTTCTCTTATGGCAGGA+TGG | 0.507870 | 1.4:-1878086 | MS.gene90695:CDS |
| TCAATCACTAACTTCATCTA+CGG | 0.513170 | 1.4:+1876438 | None:intergenic |
| TATTAAAGGGGTCCCTTACG+TGG | 0.530982 | 1.4:+1876743 | None:intergenic |
| AAAATGGGTTCTCTTATGGC+AGG | 0.548408 | 1.4:-1878090 | MS.gene90695:CDS |
| CATGCCCGTGGCCCACGTAA+GGG | 0.568293 | 1.4:-1876755 | MS.gene90695:CDS |
| ACAAATTGGAAATTGCTGTA+AGG | 0.584160 | 1.4:+1877812 | None:intergenic |
| GAAGTCAATGACCATGCCCG+TGG | 0.649977 | 1.4:-1876767 | MS.gene90695:CDS |
| CAAGTACGTTCATATTAAAG+GGG | 0.651488 | 1.4:+1876731 | None:intergenic |
| ATTAAAGGGGTCCCTTACGT+GGG | 0.678500 | 1.4:+1876744 | None:intergenic |
| CAGGTGGACTAAGAGTAACT+GGG | 0.688506 | 1.4:-1876554 | MS.gene90695:CDS |
| AATCACTAACTTCATCTACG+GGG | 0.796732 | 1.4:+1876440 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAAATAAAAACATTAAAAT+TGG | + | chr1.4:1877659-1877678 | None:intergenic | 10.0% |
| !!! | AAAAGTATAACTATAATAAT+TGG | + | chr1.4:1877875-1877894 | None:intergenic | 10.0% |
| !!! | TACAAAATCATATATATTTT+TGG | + | chr1.4:1877490-1877509 | None:intergenic | 10.0% |
| !!! | TTTATTTTTTTGGAAAAATT+TGG | + | chr1.4:1876831-1876850 | None:intergenic | 10.0% |
| !! | AATAAATAAAATACATACGA+AGG | - | chr1.4:1876845-1876864 | MS.gene90695:intron | 15.0% |
| !! | ATAAACATTAATTTCAAACT+TGG | + | chr1.4:1877702-1877721 | None:intergenic | 15.0% |
| !! | ATAATACTATAATATGACTA+AGG | - | chr1.4:1877185-1877204 | MS.gene90695:intron | 15.0% |
| !! | TAATACTATAATATGACTAA+GGG | - | chr1.4:1877186-1877205 | MS.gene90695:intron | 15.0% |
| !! | TATAGTATTATATATAGTGT+TGG | + | chr1.4:1877176-1877195 | None:intergenic | 15.0% |
| !!! | AAATACATCTTTAATTTCAT+TGG | - | chr1.4:1877000-1877019 | MS.gene90695:intron | 15.0% |
| !!! | TTAATGTTTTTATTTCATGA+TGG | - | chr1.4:1877662-1877681 | MS.gene90695:intron | 15.0% |
| !! | CATATGCATAATTCATATAT+AGG | + | chr1.4:1877925-1877944 | None:intergenic | 20.0% |
| !! | CTATATATGAATTATGCATA+TGG | - | chr1.4:1877923-1877942 | MS.gene90695:intron | 20.0% |
| !! | TTTACACACAATGTTAAAAT+TGG | + | chr1.4:1877628-1877647 | None:intergenic | 20.0% |
| !!! | AAGTATCGTTATTAATCATA+TGG | + | chr1.4:1876496-1876515 | None:intergenic | 20.0% |
| !!! | AGGTTGATATAGAAATTAAT+AGG | + | chr1.4:1877400-1877419 | None:intergenic | 20.0% |
| !!! | CTACTATTTTCTTTTTCTTT+TGG | + | chr1.4:1877144-1877163 | None:intergenic | 20.0% |
| !!! | GGTTGATATAGAAATTAATA+GGG | + | chr1.4:1877399-1877418 | None:intergenic | 20.0% |
| !!! | GTGCTTATTATTTTATGTTA+TGG | - | chr1.4:1876538-1876557 | MS.gene90695:CDS | 20.0% |
| !!! | TATATTTTTGGATTTCACTT+AGG | + | chr1.4:1877478-1877497 | None:intergenic | 20.0% |
| !!! | TATTAATTTAATTTGAGTGC+AGG | - | chr1.4:1877952-1877971 | MS.gene90695:intron | 20.0% |
| !!! | TTTTACCATTTTTCTAGTAA+TGG | - | chr1.4:1877051-1877070 | MS.gene90695:intron | 20.0% |
| ! | AAGATGAAATTGATGCTTAT+TGG | - | chr1.4:1876661-1876680 | MS.gene90695:intron | 25.0% |
| ! | ACACGACAAACATTAATTAT+TGG | - | chr1.4:1877330-1877349 | MS.gene90695:intron | 25.0% |
| ! | ACACTTGAAAAAATCAACAA+GGG | - | chr1.4:1877112-1877131 | MS.gene90695:intron | 25.0% |
| ! | ACTATAATAATTGGAAGAGT+AGG | + | chr1.4:1877866-1877885 | None:intergenic | 25.0% |
| ! | ATAAGGTAGTTATATTGATG+AGG | - | chr1.4:1876587-1876606 | MS.gene90695:intron | 25.0% |
| ! | ATTACTCTAGTAGAAGAATA+TGG | + | chr1.4:1877300-1877319 | None:intergenic | 25.0% |
| ! | GAACCCTAATAATTGTTAAT+TGG | + | chr1.4:1876918-1876937 | None:intergenic | 25.0% |
| ! | GCTCCAATTAACAATTATTA+GGG | - | chr1.4:1876912-1876931 | MS.gene90695:intron | 25.0% |
| ! | TAAGGTAGTTATATTGATGA+GGG | - | chr1.4:1876588-1876607 | MS.gene90695:intron | 25.0% |
| ! | TACACTTGAAAAAATCAACA+AGG | - | chr1.4:1877111-1877130 | MS.gene90695:intron | 25.0% |
| ! | TCAAGTACGTTCATATTAAA+GGG | + | chr1.4:1877798-1877817 | None:intergenic | 25.0% |
| ! | TGCTCCAATTAACAATTATT+AGG | - | chr1.4:1876911-1876930 | MS.gene90695:intron | 25.0% |
| ! | TTACTCTAGTAGAAGAATAT+GGG | + | chr1.4:1877299-1877318 | None:intergenic | 25.0% |
| ! | TTGAATCTAGTAAAAGAAGT+TGG | + | chr1.4:1877375-1877394 | None:intergenic | 25.0% |
| !! | AGTATTATATATAGTGTTGG+TGG | + | chr1.4:1877173-1877192 | None:intergenic | 25.0% |
| !! | TAGTTTTCTAATATGCAAGA+AGG | + | chr1.4:1877429-1877448 | None:intergenic | 25.0% |
| !!! | GTATTATATATAGTGTTGGT+GGG | + | chr1.4:1877172-1877191 | None:intergenic | 25.0% |
| !!! | TATTATATATAGTGTTGGTG+GGG | + | chr1.4:1877171-1877190 | None:intergenic | 25.0% |
| !!! | TTTTTTGGAAAAATTTGGTG+TGG | + | chr1.4:1876826-1876845 | None:intergenic | 25.0% |
| AACTTATCAACAAAAATGGC+TGG | - | chr1.4:1877832-1877851 | MS.gene90695:CDS | 30.0% | |
| AATATGCAAGAAGGACTAAA+AGG | + | chr1.4:1877420-1877439 | None:intergenic | 30.0% | |
| AATCATATGGTACCTGATTT+AGG | + | chr1.4:1876483-1876502 | None:intergenic | 30.0% | |
| ACAAATTGGAAATTGCTGTA+AGG | + | chr1.4:1876716-1876735 | None:intergenic | 30.0% | |
| AGTGACCATTACTAGAAAAA+TGG | + | chr1.4:1877059-1877078 | None:intergenic | 30.0% | |
| CAAGTACGTTCATATTAAAG+GGG | + | chr1.4:1877797-1877816 | None:intergenic | 30.0% | |
| GAAATCAAAGAAGAAGATAG+AGG | - | chr1.4:1876683-1876702 | MS.gene90695:intron | 30.0% | |
| GAGCAACTTATCAACAAAAA+TGG | - | chr1.4:1877828-1877847 | MS.gene90695:CDS | 30.0% | |
| GTCAAGTACGTTCATATTAA+AGG | + | chr1.4:1877799-1877818 | None:intergenic | 30.0% | |
| TCAATCACTAACTTCATCTA+CGG | + | chr1.4:1878090-1878109 | None:intergenic | 30.0% | |
| TCGTACTAGAACTTACTATA+AGG | - | chr1.4:1876570-1876589 | MS.gene90695:intron | 30.0% | |
| TCTTCAATTGATCCTAAATC+AGG | - | chr1.4:1876468-1876487 | MS.gene90695:CDS | 30.0% | |
| ! | TTTCATCTTTTGTAAGTGAC+TGG | + | chr1.4:1876650-1876669 | None:intergenic | 30.0% |
| !! | TAATTTAATTTGAGTGCAGG+TGG | - | chr1.4:1877955-1877974 | MS.gene90695:intron | 30.0% |
| AATCACTAACTTCATCTACG+GGG | + | chr1.4:1878088-1878107 | None:intergenic | 35.0% | |
| ATGTTTGTCGTGTCTCTAAT+AGG | + | chr1.4:1877323-1877342 | None:intergenic | 35.0% | |
| CAATCACTAACTTCATCTAC+GGG | + | chr1.4:1878089-1878108 | None:intergenic | 35.0% | |
| CAATTTGTCAGAGACTATTC+AGG | - | chr1.4:1876728-1876747 | MS.gene90695:CDS | 35.0% | |
| CTGAATAGTCTCTGACAAAT+TGG | + | chr1.4:1876730-1876749 | None:intergenic | 35.0% | |
| GAACGTACTTGACACAAATT+TGG | - | chr1.4:1877806-1877825 | MS.gene90695:CDS | 35.0% | |
| !! | TGAAACTTCTTTTCAGGATC+AGG | + | chr1.4:1877738-1877757 | None:intergenic | 35.0% |
| !! | TGACTGGTTCCTTTTAAGTA+TGG | + | chr1.4:1876634-1876653 | None:intergenic | 35.0% |
| !!! | GACTTCTGAAACTTCTTTTC+AGG | + | chr1.4:1877744-1877763 | None:intergenic | 35.0% |
| AAACTGTGCTGCATACTTGT+TGG | + | chr1.4:1878033-1878052 | None:intergenic | 40.0% | |
| AAAGAAGAAGCAGACAGAGT+TGG | + | chr1.4:1877034-1877053 | None:intergenic | 40.0% | |
| ACAGTTTCATGTTGCCAGTT+TGG | - | chr1.4:1878046-1878065 | MS.gene90695:intron | 40.0% | |
| CAGTTTCATGTTGCCAGTTT+GGG | - | chr1.4:1878047-1878066 | MS.gene90695:intron | 40.0% | |
| CTGTCTCAGCCATACTTAAA+AGG | - | chr1.4:1876622-1876641 | MS.gene90695:intron | 40.0% | |
| ! | CAATTTTGTCGATCCCAAAC+TGG | + | chr1.4:1878063-1878082 | None:intergenic | 40.0% |
| !! | ATCCAACTTATGGTTTGGTG+CGG | - | chr1.4:1876794-1876813 | MS.gene90695:intron | 40.0% |
| !! | TGACTAAGGGCACTACAATT+TGG | - | chr1.4:1877199-1877218 | MS.gene90695:intron | 40.0% |
| ATTAAAGGGGTCCCTTACGT+GGG | + | chr1.4:1877784-1877803 | None:intergenic | 45.0% | |
| GGCAGATCCAACTTATGGTT+TGG | - | chr1.4:1876789-1876808 | MS.gene90695:CDS | 45.0% | |
| TATTAAAGGGGTCCCTTACG+TGG | + | chr1.4:1877785-1877804 | None:intergenic | 45.0% | |
| TCTGAATGAGCCACCAATGA+TGG | - | chr1.4:1877998-1878017 | MS.gene90695:intron | 45.0% | |
| TTGGATGCAGCTTCCATCAT+TGG | + | chr1.4:1878014-1878033 | None:intergenic | 45.0% | |
| ! | AAGAAGCAGACAGAGTTGGT+GGG | + | chr1.4:1877030-1877049 | None:intergenic | 45.0% |
| !! | CAGGTGGACTAAGAGTAACT+GGG | - | chr1.4:1877971-1877990 | MS.gene90695:intron | 45.0% |
| !!! | ATGTATTTTATTTATTTTTT+TGG | + | chr1.4:1876841-1876860 | None:intergenic | 5.0% |
| AACCTCTACTTGCCACCTTC+CGG | - | chr1.4:1876762-1876781 | MS.gene90695:CDS | 50.0% | |
| AGCCGCACCAAACCATAAGT+TGG | + | chr1.4:1876799-1876818 | None:intergenic | 50.0% | |
| GAAGAAGCAGACAGAGTTGG+TGG | + | chr1.4:1877031-1877050 | None:intergenic | 50.0% | |
| GATGCAGCTTCCATCATTGG+TGG | + | chr1.4:1878011-1878030 | None:intergenic | 50.0% | |
| GCATGGGCAGATCCAACTTA+TGG | - | chr1.4:1876784-1876803 | MS.gene90695:CDS | 50.0% | |
| GGGTTCTCTTATGGCAGGAT+GGG | - | chr1.4:1876440-1876459 | MS.gene90695:CDS | 50.0% | |
| GGTTCTCTTATGGCAGGATG+GGG | - | chr1.4:1876441-1876460 | MS.gene90695:CDS | 50.0% | |
| TGGGTTCTCTTATGGCAGGA+TGG | - | chr1.4:1876439-1876458 | MS.gene90695:CDS | 50.0% | |
| ! | TAAGTTGGATCTGCCCATGC+CGG | + | chr1.4:1876784-1876803 | None:intergenic | 50.0% |
| !! | AGAAGCAGACAGAGTTGGTG+GGG | + | chr1.4:1877029-1877048 | None:intergenic | 50.0% |
| !! | GCAGGTGGACTAAGAGTAAC+TGG | - | chr1.4:1877970-1877989 | MS.gene90695:intron | 50.0% |
| GAAGTCAATGACCATGCCCG+TGG | - | chr1.4:1877758-1877777 | MS.gene90695:intron | 55.0% | |
| TACTTGCCACCTTCCGGCAT+GGG | - | chr1.4:1876768-1876787 | MS.gene90695:CDS | 55.0% | |
| CTACTTGCCACCTTCCGGCA+TGG | - | chr1.4:1876767-1876786 | MS.gene90695:CDS | 60.0% | |
| TGCCGGAAGGTGGCAAGTAG+AGG | + | chr1.4:1876767-1876786 | None:intergenic | 60.0% | |
| TTGGATCTGCCCATGCCGGA+AGG | + | chr1.4:1876780-1876799 | None:intergenic | 60.0% | |
| CATGCCCGTGGCCCACGTAA+GGG | - | chr1.4:1877770-1877789 | MS.gene90695:intron | 65.0% | |
| GATCTGCCCATGCCGGAAGG+TGG | + | chr1.4:1876777-1876796 | None:intergenic | 65.0% | |
| CCATGCCCGTGGCCCACGTA+AGG | - | chr1.4:1877769-1877788 | MS.gene90695:intron | 70.0% | |
| CCTTACGTGGGCCACGGGCA+TGG | + | chr1.4:1877772-1877791 | None:intergenic | 70.0% | |
| GGGGTCCCTTACGTGGGCCA+CGG | + | chr1.4:1877778-1877797 | None:intergenic | 70.0% | |
| GGGTCCCTTACGTGGGCCAC+GGG | + | chr1.4:1877777-1877796 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 1876438 | 1878109 | 1876438 | ID=MS.gene90695 |
| chr1.4 | mRNA | 1876438 | 1878109 | 1876438 | ID=MS.gene90695.t1;Parent=MS.gene90695 |
| chr1.4 | exon | 1878058 | 1878109 | 1878058 | ID=MS.gene90695.t1.exon1;Parent=MS.gene90695.t1 |
| chr1.4 | CDS | 1878058 | 1878109 | 1878058 | ID=cds.MS.gene90695.t1;Parent=MS.gene90695.t1 |
| chr1.4 | exon | 1877798 | 1877916 | 1877798 | ID=MS.gene90695.t1.exon2;Parent=MS.gene90695.t1 |
| chr1.4 | CDS | 1877798 | 1877916 | 1877798 | ID=cds.MS.gene90695.t1;Parent=MS.gene90695.t1 |
| chr1.4 | exon | 1876694 | 1876812 | 1876694 | ID=MS.gene90695.t1.exon3;Parent=MS.gene90695.t1 |
| chr1.4 | CDS | 1876694 | 1876812 | 1876694 | ID=cds.MS.gene90695.t1;Parent=MS.gene90695.t1 |
| chr1.4 | exon | 1876438 | 1876573 | 1876438 | ID=MS.gene90695.t1.exon4;Parent=MS.gene90695.t1 |
| chr1.4 | CDS | 1876438 | 1876573 | 1876438 | ID=cds.MS.gene90695.t1;Parent=MS.gene90695.t1 |
| Gene Sequence |
| Protein sequence |
