Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91084.t1 | XP_013466900.1 | 94.5 | 73 | 4 | 0 | 1 | 73 | 131 | 203 | 1.20E-31 | 145.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91084.t1 | Q9FMX7 | 77.1 | 70 | 16 | 0 | 1 | 70 | 126 | 195 | 3.2e-25 | 115.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91084.t1 | A0A072VH64 | 94.5 | 73 | 4 | 0 | 1 | 73 | 131 | 203 | 8.6e-32 | 145.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052447 | MS.gene91084 | 0.801553 | 8.53E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91084.t1 | MTR_1g038530 | 94.521 | 73 | 4 | 0 | 1 | 73 | 131 | 203 | 5.27e-46 | 145 |
| MS.gene91084.t1 | MTR_1g037830 | 94.521 | 73 | 4 | 0 | 1 | 73 | 131 | 203 | 5.62e-46 | 145 |
| MS.gene91084.t1 | MTR_1g037850 | 86.667 | 60 | 8 | 0 | 12 | 71 | 97 | 156 | 1.89e-34 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91084.t1 | AT5G23230 | 77.143 | 70 | 16 | 0 | 1 | 70 | 126 | 195 | 6.33e-35 | 117 |
| MS.gene91084.t1 | AT5G23220 | 75.714 | 70 | 17 | 0 | 1 | 70 | 126 | 195 | 6.76e-34 | 114 |
Find 15 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCCTTTATTAGAGGCTTTA+GGG | 0.250422 | 1.3:-21368569 | MS.gene91084:CDS |
| AGGCCTTTATTAGAGGCTTT+AGG | 0.281884 | 1.3:-21368570 | MS.gene91084:CDS |
| TGCAACTGCTACTTCTGATT+TGG | 0.316176 | 1.3:-21368530 | MS.gene91084:CDS |
| GCGCGTGAGGCCTTTATTAG+AGG | 0.320021 | 1.3:-21368577 | MS.gene91084:CDS |
| CACCCTAAAGCCTCTAATAA+AGG | 0.383650 | 1.3:+21368567 | None:intergenic |
| CTTACTTTGTTGATTGTCAC+AGG | 0.435237 | 1.3:-21368465 | MS.gene91084:CDS |
| GAAAGAGATGGGTGTGGAGG+AGG | 0.438319 | 1.3:-21368644 | None:intergenic |
| GAAGCTGAAAGAGATGGGTG+TGG | 0.483670 | 1.3:-21368650 | None:intergenic |
| AGAAGTAGCAGTTGCATCAG+TGG | 0.497636 | 1.3:+21368537 | None:intergenic |
| GCTGAAAGAGATGGGTGTGG+AGG | 0.519757 | 1.3:-21368647 | None:intergenic |
| GTGGAGGAGGTTATAGTGAC+GGG | 0.521658 | 1.3:-21368631 | MS.gene91084:CDS |
| TTCTGATTTGGATCTTCATG+AGG | 0.546705 | 1.3:-21368518 | MS.gene91084:CDS |
| TGTGGAGGAGGTTATAGTGA+CGG | 0.549097 | 1.3:-21368632 | MS.gene91084:CDS |
| CTGTGAGACAACGGCGCGTG+AGG | 0.563577 | 1.3:-21368590 | MS.gene91084:CDS |
| TAACTTGTGCTGTGAGACAA+CGG | 0.722054 | 1.3:-21368599 | MS.gene91084:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACATTGAAAAACATAGCTTA+TGG | - | chr1.3:21368582-21368601 | MS.gene91084:CDS | 25.0% |
| ! | CTTACTTTGTTGATTGTCAC+AGG | - | chr1.3:21368610-21368629 | MS.gene91084:CDS | 35.0% |
| !! | TTCTGATTTGGATCTTCATG+AGG | - | chr1.3:21368557-21368576 | MS.gene91084:CDS | 35.0% |
| AGGCCTTTATTAGAGGCTTT+AGG | - | chr1.3:21368505-21368524 | MS.gene91084:CDS | 40.0% | |
| CACCCTAAAGCCTCTAATAA+AGG | + | chr1.3:21368511-21368530 | None:intergenic | 40.0% | |
| GGCCTTTATTAGAGGCTTTA+GGG | - | chr1.3:21368506-21368525 | MS.gene91084:CDS | 40.0% | |
| ! | TAACTTGTGCTGTGAGACAA+CGG | - | chr1.3:21368476-21368495 | MS.gene91084:CDS | 40.0% |
| ! | TGCAACTGCTACTTCTGATT+TGG | - | chr1.3:21368545-21368564 | MS.gene91084:CDS | 40.0% |
| AGAAGTAGCAGTTGCATCAG+TGG | + | chr1.3:21368541-21368560 | None:intergenic | 45.0% | |
| TGTGGAGGAGGTTATAGTGA+CGG | - | chr1.3:21368443-21368462 | MS.gene91084:CDS | 45.0% | |
| GTGGAGGAGGTTATAGTGAC+GGG | - | chr1.3:21368444-21368463 | MS.gene91084:CDS | 50.0% | |
| GCGCGTGAGGCCTTTATTAG+AGG | - | chr1.3:21368498-21368517 | MS.gene91084:CDS | 55.0% | |
| CTGTGAGACAACGGCGCGTG+AGG | - | chr1.3:21368485-21368504 | MS.gene91084:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 21368438 | 21368659 | 21368438 | ID=MS.gene91084 |
| chr1.3 | mRNA | 21368438 | 21368659 | 21368438 | ID=MS.gene91084.t1;Parent=MS.gene91084 |
| chr1.3 | exon | 21368438 | 21368659 | 21368438 | ID=MS.gene91084.t1.exon1;Parent=MS.gene91084.t1 |
| chr1.3 | CDS | 21368438 | 21368659 | 21368438 | ID=cds.MS.gene91084.t1;Parent=MS.gene91084.t1 |
| Gene Sequence |
| Protein sequence |
