Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91136.t1 | RHN78473.1 | 82.8 | 93 | 16 | 0 | 1 | 93 | 1 | 93 | 1.60E-35 | 158.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91136.t1 | A0A396JQB5 | 82.8 | 93 | 16 | 0 | 1 | 93 | 1 | 93 | 1.2e-35 | 158.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052902 | MS.gene91136 | 0.810169 | 1.32E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91136.t1 | MTR_1g040615 | 82.418 | 91 | 16 | 0 | 3 | 93 | 1132 | 1222 | 1.20e-47 | 163 |
| MS.gene91136.t1 | MTR_1g040615 | 78.652 | 89 | 18 | 1 | 5 | 93 | 1 | 88 | 4.48e-42 | 147 |
| MS.gene91136.t1 | MTR_1g039310 | 79.570 | 93 | 18 | 1 | 1 | 93 | 1 | 92 | 1.10e-43 | 151 |
| MS.gene91136.t1 | MTR_1g038890 | 76.596 | 94 | 21 | 1 | 1 | 93 | 1 | 94 | 3.62e-43 | 150 |
| MS.gene91136.t1 | MTR_1g040525 | 77.895 | 95 | 19 | 1 | 1 | 93 | 1 | 95 | 8.02e-43 | 149 |
| MS.gene91136.t1 | MTR_0400s0030 | 67.273 | 110 | 35 | 1 | 1 | 110 | 1 | 109 | 1.15e-42 | 149 |
| MS.gene91136.t1 | MTR_0365s0020 | 67.273 | 110 | 35 | 1 | 1 | 110 | 1 | 109 | 1.15e-42 | 149 |
| MS.gene91136.t1 | MTR_1g040545 | 78.652 | 89 | 18 | 1 | 5 | 93 | 1 | 88 | 4.79e-42 | 147 |
| MS.gene91136.t1 | MTR_1g040555 | 77.381 | 84 | 19 | 0 | 10 | 93 | 1 | 84 | 1.54e-39 | 139 |
| MS.gene91136.t1 | MTR_0400s0040 | 70.526 | 95 | 27 | 1 | 1 | 95 | 1 | 94 | 2.85e-38 | 136 |
| MS.gene91136.t1 | MTR_0365s0030 | 70.526 | 95 | 27 | 1 | 1 | 95 | 1 | 94 | 2.85e-38 | 136 |
| MS.gene91136.t1 | MTR_1g039220 | 84.615 | 78 | 11 | 1 | 16 | 93 | 17 | 93 | 9.00e-37 | 132 |
| MS.gene91136.t1 | MTR_1g040575 | 76.316 | 76 | 17 | 1 | 18 | 93 | 127 | 201 | 4.69e-33 | 121 |
| MS.gene91136.t1 | MTR_1g039090 | 64.773 | 88 | 27 | 1 | 10 | 93 | 1 | 88 | 2.64e-30 | 112 |
| MS.gene91136.t1 | MTR_1g039240 | 70.270 | 74 | 21 | 1 | 22 | 95 | 1 | 73 | 2.46e-27 | 105 |
| MS.gene91136.t1 | MTR_7g045710 | 59.140 | 93 | 36 | 2 | 2 | 93 | 8 | 99 | 1.04e-22 | 92.0 |
| MS.gene91136.t1 | MTR_7g045510 | 60.215 | 93 | 35 | 2 | 2 | 93 | 8 | 99 | 2.72e-22 | 90.9 |
| MS.gene91136.t1 | MTR_7g039330 | 60.215 | 93 | 35 | 2 | 2 | 93 | 8 | 99 | 7.06e-22 | 89.7 |
| MS.gene91136.t1 | MTR_7g045860 | 53.261 | 92 | 34 | 2 | 2 | 93 | 8 | 90 | 1.88e-20 | 85.5 |
| MS.gene91136.t1 | MTR_7g446160 | 58.750 | 80 | 27 | 2 | 28 | 102 | 26 | 104 | 3.01e-20 | 85.1 |
| MS.gene91136.t1 | MTR_0289s0040 | 56.250 | 80 | 29 | 2 | 28 | 102 | 27 | 105 | 9.77e-18 | 77.8 |
| MS.gene91136.t1 | MTR_7g051040 | 48.913 | 92 | 45 | 2 | 2 | 93 | 8 | 97 | 3.37e-17 | 76.3 |
| MS.gene91136.t1 | MTR_7g081570 | 49.383 | 81 | 37 | 3 | 14 | 93 | 1 | 78 | 2.00e-15 | 71.2 |
| MS.gene91136.t1 | MTR_7g081720 | 50.000 | 82 | 35 | 2 | 26 | 102 | 28 | 108 | 2.30e-15 | 70.9 |
| MS.gene91136.t1 | MTR_7g446190 | 74.468 | 47 | 11 | 1 | 36 | 82 | 25 | 70 | 1.90e-14 | 68.6 |
| MS.gene91136.t1 | MTR_7g081480 | 43.590 | 78 | 42 | 2 | 17 | 93 | 6 | 82 | 2.38e-12 | 62.4 |
| MS.gene91136.t1 | MTR_7g081780 | 44.318 | 88 | 43 | 5 | 8 | 93 | 1 | 84 | 1.35e-11 | 60.1 |
| MS.gene91136.t1 | MTR_5g085700 | 46.154 | 78 | 37 | 4 | 17 | 93 | 28 | 101 | 3.14e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCACCAATATTGGACTAAA+AGG | 0.226348 | 1.3:-22444046 | MS.gene91136:intron |
| GAGATCAAGATAAGATAATT+TGG | 0.269404 | 1.3:+22443847 | None:intergenic |
| GCAATGCTTTGCTCTCTTCA+TGG | 0.313641 | 1.3:-22444144 | MS.gene91136:CDS |
| AAGGTAAATCTCACCAATAT+TGG | 0.354623 | 1.3:-22444055 | MS.gene91136:CDS |
| GTAATAATCCTTGTAGTAGT+TGG | 0.372647 | 1.3:-22444117 | MS.gene91136:CDS |
| GGTGAGATTTACCTTGTAGA+TGG | 0.383385 | 1.3:+22444063 | None:intergenic |
| ACCAATTGGGTTATCTCAAA+TGG | 0.418791 | 1.3:+22443797 | None:intergenic |
| CTCAGTGACAATTCTCTCAC+TGG | 0.446431 | 1.3:-22443827 | MS.gene91136:CDS |
| GCTTTGCTCTCTTCATGGAT+TGG | 0.455890 | 1.3:-22444139 | MS.gene91136:CDS |
| AAAAGAAGAAGGCACGAGAT+TGG | 0.470865 | 1.3:+22444271 | None:intergenic |
| TAATAATCCTTGTAGTAGTT+GGG | 0.474523 | 1.3:-22444116 | MS.gene91136:CDS |
| AAGCTGATGCTCTGTTGAAG+TGG | 0.541853 | 1.3:-22444189 | MS.gene91136:CDS |
| GGTACCATTCCCAGTAGCAT+TGG | 0.555044 | 1.3:-22443878 | MS.gene91136:intron |
| ATTCCTTCCCAACTACTACA+AGG | 0.556952 | 1.3:+22444109 | None:intergenic |
| AAGCATTGCTATGGTTGTCA+AGG | 0.567884 | 1.3:+22444158 | None:intergenic |
| AAGAGAGCAAAGCATTGCTA+TGG | 0.576948 | 1.3:+22444149 | None:intergenic |
| AATCCTTGTAGTAGTTGGGA+AGG | 0.591361 | 1.3:-22444112 | MS.gene91136:CDS |
| TGCCGCAGCATAAGGTGATG+TGG | 0.599139 | 1.3:+22444228 | None:intergenic |
| TGATTCAAAATCCATCTACA+AGG | 0.615419 | 1.3:-22444074 | MS.gene91136:CDS |
| GGCCACATCACCTTATGCTG+CGG | 0.621448 | 1.3:-22444230 | MS.gene91136:CDS |
| TCAGTGACAATTCTCTCACT+GGG | 0.621481 | 1.3:-22443826 | MS.gene91136:CDS |
| ATGTGGCCATAACAAACACA+TGG | 0.637077 | 1.3:+22444245 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAGATCAAGATAAGATAATT+TGG | + | chr1.3:22444258-22444277 | None:intergenic | 25.0% |
| ! | TAATAATCCTTGTAGTAGTT+GGG | - | chr1.3:22443986-22444005 | MS.gene91136:intron | 25.0% |
| ! | TTAAAAGACAACTCTTTCTA+CGG | - | chr1.3:22444131-22444150 | MS.gene91136:CDS | 25.0% |
| ! | TTGTCACTGAATAATTTCTA+TGG | - | chr1.3:22444203-22444222 | MS.gene91136:CDS | 25.0% |
| !!! | AGAGTTGTCTTTTAAAACTA+AGG | + | chr1.3:22444126-22444145 | None:intergenic | 25.0% |
| !!! | TTTTTTCCATGTGTTTGTTA+TGG | - | chr1.3:22443851-22443870 | MS.gene91136:CDS | 25.0% |
| AAGGTAAATCTCACCAATAT+TGG | - | chr1.3:22444047-22444066 | MS.gene91136:CDS | 30.0% | |
| ACACATGGAAAAAAAGAAGA+AGG | + | chr1.3:22443845-22443864 | None:intergenic | 30.0% | |
| GTAATAATCCTTGTAGTAGT+TGG | - | chr1.3:22443985-22444004 | MS.gene91136:intron | 30.0% | |
| TGATTCAAAATCCATCTACA+AGG | - | chr1.3:22444028-22444047 | MS.gene91136:intron | 30.0% | |
| ! | TGTACCTTTTAGTCCAATAT+TGG | + | chr1.3:22444063-22444082 | None:intergenic | 30.0% |
| !!! | TGTCTTTTAAAACTAAGGTG+TGG | + | chr1.3:22444121-22444140 | None:intergenic | 30.0% |
| AGTTGTACCACACAATATTG+GGG | - | chr1.3:22444154-22444173 | MS.gene91136:CDS | 35.0% | |
| CTCACCAATATTGGACTAAA+AGG | - | chr1.3:22444056-22444075 | MS.gene91136:CDS | 35.0% | |
| GACAATTTACCAATGCTACT+GGG | + | chr1.3:22444236-22444255 | None:intergenic | 35.0% | |
| GAGTTGTACCACACAATATT+GGG | - | chr1.3:22444153-22444172 | MS.gene91136:CDS | 35.0% | |
| !! | CAAATCAAGAGTGTCTAGAT+TGG | + | chr1.3:22444186-22444205 | None:intergenic | 35.0% |
| AATCCTTGTAGTAGTTGGGA+AGG | - | chr1.3:22443990-22444009 | MS.gene91136:intron | 40.0% | |
| ATGTGGCCATAACAAACACA+TGG | + | chr1.3:22443860-22443879 | None:intergenic | 40.0% | |
| ATTCCTTCCCAACTACTACA+AGG | + | chr1.3:22443996-22444015 | None:intergenic | 40.0% | |
| GGACAATTTACCAATGCTAC+TGG | + | chr1.3:22444237-22444256 | None:intergenic | 40.0% | |
| GGAGTTGTACCACACAATAT+TGG | - | chr1.3:22444152-22444171 | MS.gene91136:CDS | 40.0% | |
| GGTGAGATTTACCTTGTAGA+TGG | + | chr1.3:22444042-22444061 | None:intergenic | 40.0% | |
| TCAGTGACAATTCTCTCACT+GGG | - | chr1.3:22444276-22444295 | MS.gene91136:CDS | 40.0% | |
| TTTACCAATGCTACTGGGAA+TGG | + | chr1.3:22444231-22444250 | None:intergenic | 40.0% | |
| ! | AAAAGAAGAAGGCACGAGAT+TGG | + | chr1.3:22443834-22443853 | None:intergenic | 40.0% |
| ! | AAGCATTGCTATGGTTGTCA+AGG | + | chr1.3:22443947-22443966 | None:intergenic | 40.0% |
| !! | AAGAGAGCAAAGCATTGCTA+TGG | + | chr1.3:22443956-22443975 | None:intergenic | 40.0% |
| AAGCTGATGCTCTGTTGAAG+TGG | - | chr1.3:22443913-22443932 | MS.gene91136:intron | 45.0% | |
| CTCAGTGACAATTCTCTCAC+TGG | - | chr1.3:22444275-22444294 | MS.gene91136:CDS | 45.0% | |
| GACATCTCCCCAATATTGTG+TGG | + | chr1.3:22444164-22444183 | None:intergenic | 45.0% | |
| GCAATGCTTTGCTCTCTTCA+TGG | - | chr1.3:22443958-22443977 | MS.gene91136:intron | 45.0% | |
| GCTTTGCTCTCTTCATGGAT+TGG | - | chr1.3:22443963-22443982 | MS.gene91136:intron | 45.0% | |
| GGTACCATTCCCAGTAGCAT+TGG | - | chr1.3:22444224-22444243 | MS.gene91136:CDS | 50.0% | |
| ! | TGCACTTTTGCCGCAGCATA+AGG | + | chr1.3:22443885-22443904 | None:intergenic | 50.0% |
| GGCCACATCACCTTATGCTG+CGG | - | chr1.3:22443872-22443891 | MS.gene91136:intron | 55.0% | |
| TGCCGCAGCATAAGGTGATG+TGG | + | chr1.3:22443877-22443896 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 22443807 | 22444317 | 22443807 | ID=MS.gene91136 |
| chr1.3 | mRNA | 22443807 | 22444317 | 22443807 | ID=MS.gene91136.t1;Parent=MS.gene91136 |
| chr1.3 | exon | 22444047 | 22444317 | 22444047 | ID=MS.gene91136.t1.exon1;Parent=MS.gene91136.t1 |
| chr1.3 | CDS | 22444047 | 22444317 | 22444047 | ID=cds.MS.gene91136.t1;Parent=MS.gene91136.t1 |
| chr1.3 | exon | 22443807 | 22443886 | 22443807 | ID=MS.gene91136.t1.exon2;Parent=MS.gene91136.t1 |
| chr1.3 | CDS | 22443807 | 22443886 | 22443807 | ID=cds.MS.gene91136.t1;Parent=MS.gene91136.t1 |
| Gene Sequence |
| Protein sequence |
