AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91239

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91239.t1 MTR_5g074600 80.496 282 54 1 1 281 78 359 9.49e-168 469
MS.gene91239.t1 MTR_5g074540 83.333 282 28 2 1 281 78 341 3.88e-167 466
MS.gene91239.t1 MTR_5g074580 78.369 282 60 1 1 281 79 360 1.99e-162 455
MS.gene91239.t1 MTR_5g074680 78.648 281 59 1 1 280 79 359 1.43e-160 451
MS.gene91239.t1 MTR_5g074610 64.184 282 71 3 1 281 74 326 1.50e-127 365
MS.gene91239.t1 MTR_4g079160 59.717 283 108 4 1 281 77 355 1.52e-116 339
MS.gene91239.t1 MTR_4g088200 47.902 286 142 6 1 281 78 361 1.49e-83 255
MS.gene91239.t1 MTR_7g014570 45.745 282 145 6 1 281 78 352 2.59e-83 254
MS.gene91239.t1 MTR_4g033015 48.611 288 139 6 1 281 78 363 3.57e-81 249
MS.gene91239.t1 MTR_4g033200 48.611 288 139 6 1 281 78 363 5.05e-81 249
MS.gene91239.t1 MTR_7g014510 43.158 285 153 6 1 281 78 357 8.70e-80 245
MS.gene91239.t1 MTR_4g088190 49.135 289 137 6 1 281 78 364 1.25e-78 243
MS.gene91239.t1 MTR_4g033150 46.875 288 144 6 1 281 78 363 5.65e-78 241
MS.gene91239.t1 MTR_4g088345 44.964 278 147 5 6 281 83 356 1.61e-75 234
MS.gene91239.t1 MTR_4g088340 44.964 278 147 5 6 281 60 333 2.03e-75 233
MS.gene91239.t1 MTR_7g024520 45.000 260 136 4 21 279 95 348 2.33e-73 229
MS.gene91239.t1 MTR_1g076940 42.509 287 156 7 1 281 78 361 3.84e-72 226
MS.gene91239.t1 MTR_7g024500 43.077 260 141 4 21 279 95 348 9.11e-72 224
MS.gene91239.t1 MTR_4g033170 46.479 284 145 4 1 281 78 357 9.76e-71 222
MS.gene91239.t1 MTR_4g033085 45.775 284 147 4 1 281 78 357 6.45e-67 212
MS.gene91239.t1 MTR_3g083620 38.489 278 168 3 1 277 82 357 2.32e-59 193
MS.gene91239.t1 MTR_0079s0070 39.700 267 155 5 18 281 71 334 4.97e-56 184
MS.gene91239.t1 MTR_4g038440 39.700 267 155 5 18 281 105 368 1.61e-55 183
MS.gene91239.t1 MTR_0017s0100 38.577 267 158 4 18 281 105 368 3.63e-51 172
MS.gene91239.t1 MTR_1g096310 33.937 221 142 3 48 268 142 358 3.75e-44 154
MS.gene91239.t1 MTR_8g068870 32.950 261 170 4 21 280 106 362 5.18e-42 148
MS.gene91239.t1 MTR_0591s0010 36.885 244 147 6 21 262 120 358 5.28e-42 148
MS.gene91239.t1 MTR_1g036490 38.500 200 119 3 69 268 160 355 1.76e-41 147
MS.gene91239.t1 MTR_8g068690 34.979 243 153 4 21 262 119 357 2.84e-41 146
MS.gene91239.t1 MTR_2g055940 35.185 216 130 4 54 266 139 347 3.01e-41 146
MS.gene91239.t1 MTR_7g118320 36.000 250 149 7 21 266 105 347 3.49e-41 145
MS.gene91239.t1 MTR_1g036460 38.095 210 126 3 59 268 150 355 1.35e-40 144
MS.gene91239.t1 MTR_8g068750 33.603 247 159 4 21 266 120 362 1.88e-40 144
MS.gene91239.t1 MTR_7g012040 33.636 220 142 3 48 267 138 353 2.71e-40 143
MS.gene91239.t1 MTR_7g011990 33.182 220 143 3 48 267 137 352 2.74e-40 143
MS.gene91239.t1 MTR_7g012070 32.127 221 146 3 48 268 145 361 5.45e-40 143
MS.gene91239.t1 MTR_8g024160 34.538 249 151 6 36 280 87 327 1.09e-39 141
MS.gene91239.t1 MTR_1g036510 38.500 200 119 3 69 268 174 369 1.34e-39 142
MS.gene91239.t1 MTR_7g012090 33.182 220 143 3 48 267 145 360 3.75e-39 140
MS.gene91239.t1 MTR_7g011900 33.488 215 139 2 54 268 146 356 3.90e-39 140
MS.gene91239.t1 MTR_7g118300 34.375 256 159 7 17 270 114 362 3.42e-38 138
MS.gene91239.t1 MTR_3g092900 33.333 252 155 8 21 267 106 349 6.50e-38 137
MS.gene91239.t1 MTR_3g021440 33.790 219 140 3 50 268 143 356 1.78e-37 136
MS.gene91239.t1 MTR_5g098170 34.091 220 135 5 52 268 138 350 4.65e-37 135
MS.gene91239.t1 MTR_7g012020 31.944 216 139 4 54 267 145 354 1.97e-36 133
MS.gene91239.t1 MTR_3g021430 32.489 237 151 5 33 267 113 342 3.74e-36 132
MS.gene91239.t1 MTR_6g078850 32.941 255 162 7 15 266 120 368 2.09e-35 133
MS.gene91239.t1 MTR_0794s0020 37.572 173 101 4 1 167 78 249 7.83e-35 126
MS.gene91239.t1 MTR_1g076930 36.413 184 113 3 1 181 55 237 4.27e-33 121
MS.gene91239.t1 MTR_8g068570 40.361 166 94 4 84 248 156 317 2.20e-31 119
MS.gene91239.t1 MTR_8g068730 31.200 250 129 8 21 266 120 330 5.88e-29 113
MS.gene91239.t1 MTR_4g038440 36.145 166 104 1 18 181 105 270 1.61e-27 107
MS.gene91239.t1 MTR_2g072790 30.435 253 168 6 18 266 91 339 1.82e-26 106
MS.gene91239.t1 MTR_3g096070 28.704 216 147 4 54 266 109 320 2.39e-23 97.8
MS.gene91239.t1 MTR_3g096050 28.137 263 161 9 18 266 95 343 4.06e-22 94.7
MS.gene91239.t1 MTR_1g050220 32.903 155 91 3 117 271 152 293 1.40e-21 92.4
MS.gene91239.t1 MTR_8g068600 30.864 162 109 3 21 181 94 253 2.63e-20 88.6
MS.gene91239.t1 MTR_7g012020 32.812 128 85 1 54 181 145 271 4.65e-20 88.2
MS.gene91239.t1 MTR_8g068620 36.283 113 71 1 72 184 2 113 4.80e-20 83.6
MS.gene91239.t1 MTR_8g068690 30.909 165 111 3 21 184 119 281 1.62e-19 86.3
MS.gene91239.t1 MTR_1g036510 38.938 113 68 1 69 181 174 285 2.78e-19 85.9
MS.gene91239.t1 MTR_4g025570 42.708 96 51 4 183 277 122 214 2.50e-14 70.9
MS.gene91239.t1 MTR_8g068740 26.705 176 94 3 21 195 120 261 4.27e-14 71.2
MS.gene91239.t1 MTR_7g012020 33.333 99 65 1 54 152 145 242 8.69e-13 67.0
MS.gene91239.t1 MTR_1g036510 38.824 85 51 1 69 153 174 257 1.79e-12 66.2
MS.gene91239.t1 MTR_3g096050 27.586 174 107 5 18 181 95 259 1.94e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91239.t1 AT4G35160 35.154 293 175 7 1 280 90 380 1.53e-46 160
MS.gene91239.t1 AT4G35150 40.000 215 121 4 73 280 110 323 3.31e-45 155
MS.gene91239.t1 AT1G51990 35.094 265 163 6 21 281 103 362 5.31e-44 153
MS.gene91239.t1 AT1G51990 35.094 265 163 6 21 281 103 362 5.96e-44 153
MS.gene91239.t1 AT1G77520 34.348 230 142 4 57 280 154 380 4.37e-39 140
MS.gene91239.t1 AT5G54160 33.080 263 167 6 8 267 91 347 2.63e-38 138
MS.gene91239.t1 AT5G53810 37.383 214 130 3 68 280 167 377 2.64e-35 130
MS.gene91239.t1 AT1G63140 39.485 233 132 6 51 280 154 380 1.12e-34 129
MS.gene91239.t1 AT1G63140 39.485 233 132 6 51 280 174 400 1.26e-34 129
MS.gene91239.t1 AT1G77530 35.981 214 133 3 68 280 170 380 1.06e-32 124
MS.gene91239.t1 AT3G53140 30.882 272 174 8 1 266 81 344 1.02e-31 120
MS.gene91239.t1 AT1G33030 32.636 239 152 7 32 267 104 336 9.61e-31 118
MS.gene91239.t1 AT1G62900 38.942 208 121 4 75 280 1 204 3.09e-30 113
MS.gene91239.t1 AT5G37170 33.668 199 127 4 84 280 38 233 3.35e-30 114
MS.gene91239.t1 AT5G37170 33.668 199 127 4 84 280 138 333 1.81e-29 114
MS.gene91239.t1 AT5G37170 34.043 188 119 4 95 280 1 185 3.86e-28 107
MS.gene91239.t1 AT1G21130 31.321 265 163 8 12 267 105 359 7.01e-28 110
MS.gene91239.t1 AT1G21100 34.146 205 122 5 68 267 163 359 3.79e-27 108
MS.gene91239.t1 AT1G21110 34.328 201 119 5 68 263 163 355 1.10e-26 107
MS.gene91239.t1 AT1G21120 33.659 205 123 5 68 267 163 359 4.36e-26 105
MS.gene91239.t1 AT1G21120 33.659 205 123 5 68 267 211 407 6.65e-26 105
MS.gene91239.t1 AT1G76790 33.000 200 121 5 68 262 155 346 1.34e-24 101
MS.gene91239.t1 AT1G63140 37.121 132 79 3 51 181 174 302 3.55e-15 74.7
MS.gene91239.t1 AT1G33030 29.801 151 103 3 32 181 104 252 4.90e-12 65.1
MS.gene91239.t1 AT1G33030 29.801 151 103 3 32 181 104 252 6.40e-12 64.7

Find 55 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAAATGAGTTGTTAATTT+TGG 0.160023 5.1:-57224879 None:intergenic
CAGCACATGGGATGTCGTTT+TGG 0.230646 5.1:+57224837 MS.gene91239:CDS
ATAGACCTTGATCCTAAAAT+TGG 0.254900 5.1:-57226059 None:intergenic
GATCTCCCACATGTTGTTCA+TGG 0.264137 5.1:+57225064 MS.gene91239:CDS
TGCCATGGATCTATCATATC+TGG 0.330412 5.1:-57224767 None:intergenic
ATGCGAATCATGACTTATTC+CGG 0.334688 5.1:+57224617 None:intergenic
TACAGTCGATATTGCATGAT+TGG 0.335498 5.1:+57225793 MS.gene91239:intron
CTCCATTTGTGGGAATGATT+TGG 0.349338 5.1:-57225029 None:intergenic
CAACCAATGACTCAACTTCA+TGG 0.372843 5.1:-57224967 None:intergenic
AACAAAATTGCATCTGTTGA+CGG 0.377379 5.1:-57225142 None:intergenic
TCGATTAGTTTCCGATTTGT+TGG 0.382710 5.1:+57224922 MS.gene91239:CDS
GAAAACTTAAACTATGTTGC+TGG 0.385861 5.1:+57225100 MS.gene91239:CDS
GTTGATGTTGGAGGAGGTAC+GGG 0.411565 5.1:+57224986 MS.gene91239:CDS
TGCCAAATCATTCCCACAAA+TGG 0.419064 5.1:+57225027 MS.gene91239:CDS
AAGAGAGAAACGAGAAAGAA+TGG 0.424421 5.1:+57225991 MS.gene91239:CDS
ATGGATTAAGTTGATTTCCT+TGG 0.431482 5.1:+57226010 MS.gene91239:CDS
GTTCCATGAAGTTGAGTCAT+TGG 0.432542 5.1:+57224964 MS.gene91239:CDS
CAACTCATTTAACGAATCAA+TGG 0.437712 5.1:+57224889 MS.gene91239:CDS
CATGTTGTTCATGGTTTGCA+AGG 0.449071 5.1:+57225073 MS.gene91239:CDS
AACACAATGCACTCCATTTG+TGG 0.453290 5.1:-57225040 None:intergenic
GCAATATCAAAGAAAGGTAA+AGG 0.460727 5.1:+57225858 MS.gene91239:CDS
GCACTTCTCAACCAACAAAT+CGG 0.467046 5.1:-57224933 None:intergenic
CATGGCATCAATTCTCTGCT+TGG 0.468036 5.1:+57224783 MS.gene91239:CDS
GTTGAGTCATTGGTTGATGT+TGG 0.468409 5.1:+57224974 MS.gene91239:CDS
ATTAAGTTGATTTCCTTGGC+TGG 0.468848 5.1:+57226014 MS.gene91239:CDS
GGTTGATGTTGGAGGAGGTA+CGG 0.474379 5.1:+57224985 MS.gene91239:CDS
ACACAATGCACTCCATTTGT+GGG 0.515239 5.1:-57225039 None:intergenic
ACATGTGGTGCCACACTCAT+TGG 0.525895 5.1:-57224728 None:intergenic
CAATATCAAAGAAAGGTAAA+GGG 0.532814 5.1:+57225859 MS.gene91239:CDS
TTGATGATGGTAGTATTCGC+AGG 0.543043 5.1:+57225966 MS.gene91239:CDS
AAGGATGCAATATCAAAGAA+AGG 0.545671 5.1:+57225852 MS.gene91239:CDS
CTTCTATGACATGTTGATGA+TGG 0.553245 5.1:+57225953 MS.gene91239:CDS
ACGGTGTTAGACAAAGAGAA+TGG 0.563271 5.1:+57225900 MS.gene91239:CDS
GAGTCATTGGTTGATGTTGG+AGG 0.570761 5.1:+57224977 MS.gene91239:CDS
TGCAAACCATGAACAACATG+TGG 0.579903 5.1:-57225070 None:intergenic
AAAGAAAGGTAAAGGGAAGG+TGG 0.580286 5.1:+57225866 MS.gene91239:CDS
TGGAGGAGGTACGGGGACCA+TGG 0.588947 5.1:+57224994 MS.gene91239:CDS
CAAGCAGAGAATTGATGCCA+TGG 0.591666 5.1:-57224782 None:intergenic
CTGAGTATCCCGTGCACATG+TGG 0.594919 5.1:-57224743 None:intergenic
GTAGTCACTAAAACCAGCCA+AGG 0.595683 5.1:-57226027 None:intergenic
TTGTTGGTTGAGAAGTGCAA+GGG 0.598365 5.1:+57224938 MS.gene91239:CDS
TTTGTTGGTTGAGAAGTGCA+AGG 0.604264 5.1:+57224937 MS.gene91239:CDS
GTCCAGATATGATAGATCCA+TGG 0.611089 5.1:+57224765 MS.gene91239:CDS
AAAGATATTAAAGAATTGCA+AGG 0.612054 5.1:+57225833 MS.gene91239:CDS
ATCAAAGAAAGGTAAAGGGA+AGG 0.618639 5.1:+57225863 MS.gene91239:CDS
TCATTGGTTGATGTTGGAGG+AGG 0.622074 5.1:+57224980 MS.gene91239:CDS
TTGATGTTGGAGGAGGTACG+GGG 0.638274 5.1:+57224987 MS.gene91239:CDS
GTGTGGCACCACATGTGCAC+GGG 0.643351 5.1:+57224735 MS.gene91239:CDS
ACAGCATTTGAAACAGCACA+TGG 0.645064 5.1:+57224824 MS.gene91239:CDS
AGTGTGGCACCACATGTGCA+CGG 0.654606 5.1:+57224734 MS.gene91239:CDS
TAAGAACAGTCCAATGAGTG+TGG 0.672166 5.1:+57224718 MS.gene91239:CDS
GCAAACCATGAACAACATGT+GGG 0.683754 5.1:-57225069 None:intergenic
CAGCATTTGAAACAGCACAT+GGG 0.692682 5.1:+57224825 MS.gene91239:CDS
GAAGGTGGTTATCATTGACA+CGG 0.700974 5.1:+57225881 MS.gene91239:CDS
ATTGCATGATTGGAATGACG+AGG 0.708926 5.1:+57225803 MS.gene91239:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTGATTTTTTTTTCT+TGG + chr5.1:57225616-57225635 MS.gene91239:intron 10.0%
!! ACAAACATATATATTCTTAA+TGG - chr5.1:57225270-57225289 None:intergenic 15.0%
!! ATATATATAATTGTTCTCAT+GGG - chr5.1:57225495-57225514 None:intergenic 15.0%
!! ATTAAGAATATATATGTTTG+TGG + chr5.1:57225269-57225288 MS.gene91239:intron 15.0%
!! TATATATATAATTGTTCTCA+TGG - chr5.1:57225496-57225515 None:intergenic 15.0%
!! TTAAGAATATATATGTTTGT+GGG + chr5.1:57225270-57225289 MS.gene91239:intron 15.0%
!!! AAAATTAGTTTACACATTTT+AGG + chr5.1:57225592-57225611 MS.gene91239:intron 15.0%
!!! AAATTAGTTTACACATTTTA+GGG + chr5.1:57225593-57225612 MS.gene91239:intron 15.0%
!! AAAGATATTAAAGAATTGCA+AGG + chr5.1:57225833-57225852 MS.gene91239:CDS 20.0%
!! TATATATAATTGTTCTCATG+GGG - chr5.1:57225494-57225513 None:intergenic 20.0%
!!! CCATATTTTTCTACTAATAA+CGG + chr5.1:57225670-57225689 MS.gene91239:intron 20.0%
!!! GAACATTAGAGTATTTTTTA+TGG - chr5.1:57225232-57225251 None:intergenic 20.0%
!!! GTAAACTAATTTTTTTACCA+TGG - chr5.1:57225585-57225604 None:intergenic 20.0%
!!! GTTAAATGAGTTGTTAATTT+TGG - chr5.1:57224882-57224901 None:intergenic 20.0%
! ATATATAATTGTTCTCATGG+GGG - chr5.1:57225493-57225512 None:intergenic 25.0%
! ATATATGTTTGTGGGTTTAA+TGG + chr5.1:57225278-57225297 MS.gene91239:intron 25.0%
! CAATATCAAAGAAAGGTAAA+GGG + chr5.1:57225859-57225878 MS.gene91239:CDS 25.0%
! CCGTTATTAGTAGAAAAATA+TGG - chr5.1:57225673-57225692 None:intergenic 25.0%
! CGAGGAAAATATTATCTAAT+TGG - chr5.1:57225696-57225715 None:intergenic 25.0%
! TTTATTATCCATAGAGATAG+AGG + chr5.1:57225320-57225339 MS.gene91239:intron 25.0%
!! AATTTAGGCAAACATAGTTT+TGG - chr5.1:57225556-57225575 None:intergenic 25.0%
!! TACAAGATTACTCCAATTTT+AGG + chr5.1:57226047-57226066 MS.gene91239:CDS 25.0%
!!! TTTTTTCTTGGTACAAAAGA+GGG + chr5.1:57225628-57225647 MS.gene91239:intron 25.0%
!!! TTTTTTTCTTGGTACAAAAG+AGG + chr5.1:57225627-57225646 MS.gene91239:intron 25.0%
AACAAAATTGCATCTGTTGA+CGG - chr5.1:57225145-57225164 None:intergenic 30.0%
AAGGATGCAATATCAAAGAA+AGG + chr5.1:57225852-57225871 MS.gene91239:CDS 30.0%
AGATGAAAGTAGAAGCAAAA+TGG - chr5.1:57225403-57225422 None:intergenic 30.0%
AGTGGAAAAAATAGAAAACG+AGG - chr5.1:57225714-57225733 None:intergenic 30.0%
ATAGACCTTGATCCTAAAAT+TGG - chr5.1:57226062-57226081 None:intergenic 30.0%
CAACTCATTTAACGAATCAA+TGG + chr5.1:57224889-57224908 MS.gene91239:CDS 30.0%
GAAAACTTAAACTATGTTGC+TGG + chr5.1:57225100-57225119 MS.gene91239:CDS 30.0%
GAAAAGAGAACAAACTAAAG+TGG - chr5.1:57225732-57225751 None:intergenic 30.0%
GCAATATCAAAGAAAGGTAA+AGG + chr5.1:57225858-57225877 MS.gene91239:CDS 30.0%
TTACCATGGTGACATAATTT+AGG - chr5.1:57225571-57225590 None:intergenic 30.0%
! AACAGATGCAATTTTGTTGA+AGG + chr5.1:57225147-57225166 MS.gene91239:CDS 30.0%
! ATTGTTTTCCTCTATCTCTA+TGG - chr5.1:57225331-57225350 None:intergenic 30.0%
! TATGTTTGAGTTACGAAAGA+AGG - chr5.1:57225760-57225779 None:intergenic 30.0%
! TTTTTCTTGGTACAAAAGAG+GGG + chr5.1:57225629-57225648 MS.gene91239:intron 30.0%
!! ATGGATTAAGTTGATTTCCT+TGG + chr5.1:57226010-57226029 MS.gene91239:CDS 30.0%
!!! TTACTCCAATTTTAGGATCA+AGG + chr5.1:57226054-57226073 MS.gene91239:CDS 30.0%
AAGAGAGAAACGAGAAAGAA+TGG + chr5.1:57225991-57226010 MS.gene91239:CDS 35.0%
ATCAAAGAAAGGTAAAGGGA+AGG + chr5.1:57225863-57225882 MS.gene91239:CDS 35.0%
ATGCATTCCCAACATAAGTT+GGG - chr5.1:57225461-57225480 None:intergenic 35.0%
CTTGCTACTAGAAAAGAGTA+GGG + chr5.1:57225430-57225449 MS.gene91239:intron 35.0%
TACAGTCGATATTGCATGAT+TGG + chr5.1:57225793-57225812 MS.gene91239:intron 35.0%
TCTTGCTACTAGAAAAGAGT+AGG + chr5.1:57225429-57225448 MS.gene91239:intron 35.0%
TTGCCTAAATTATGTCACCA+TGG + chr5.1:57225565-57225584 MS.gene91239:intron 35.0%
! ATTTTGTTGAGAGAAGAAGC+CGG - chr5.1:57224639-57224658 None:intergenic 35.0%
! CTTCTATGACATGTTGATGA+TGG + chr5.1:57225953-57225972 MS.gene91239:CDS 35.0%
!! ATTAAGTTGATTTCCTTGGC+TGG + chr5.1:57226014-57226033 MS.gene91239:CDS 35.0%
!! TCGATTAGTTTCCGATTTGT+TGG + chr5.1:57224922-57224941 MS.gene91239:CDS 35.0%
AAAGAAAGGTAAAGGGAAGG+TGG + chr5.1:57225866-57225885 MS.gene91239:CDS 40.0%
AACACAATGCACTCCATTTG+TGG - chr5.1:57225043-57225062 None:intergenic 40.0%
ACACAATGCACTCCATTTGT+GGG - chr5.1:57225042-57225061 None:intergenic 40.0%
ATTGCATGATTGGAATGACG+AGG + chr5.1:57225803-57225822 MS.gene91239:CDS 40.0%
CAACCAATGACTCAACTTCA+TGG - chr5.1:57224970-57224989 None:intergenic 40.0%
CATACCATTGTACCACTCAT+TGG + chr5.1:57225367-57225386 MS.gene91239:intron 40.0%
CATGCATTCCCAACATAAGT+TGG - chr5.1:57225462-57225481 None:intergenic 40.0%
CTCCATTTGTGGGAATGATT+TGG - chr5.1:57225032-57225051 None:intergenic 40.0%
GAAGGTGGTTATCATTGACA+CGG + chr5.1:57225881-57225900 MS.gene91239:CDS 40.0%
GAGTGGTACAATGGTATGAT+TGG - chr5.1:57225365-57225384 None:intergenic 40.0%
GCAAACCATGAACAACATGT+GGG - chr5.1:57225072-57225091 None:intergenic 40.0%
GCACTTCTCAACCAACAAAT+CGG - chr5.1:57224936-57224955 None:intergenic 40.0%
GTCCAGATATGATAGATCCA+TGG + chr5.1:57224765-57224784 MS.gene91239:CDS 40.0%
GTTCCATGAAGTTGAGTCAT+TGG + chr5.1:57224964-57224983 MS.gene91239:CDS 40.0%
TAAGAACAGTCCAATGAGTG+TGG + chr5.1:57224718-57224737 MS.gene91239:CDS 40.0%
TGCAAACCATGAACAACATG+TGG - chr5.1:57225073-57225092 None:intergenic 40.0%
TGCCAAATCATTCCCACAAA+TGG + chr5.1:57225027-57225046 MS.gene91239:CDS 40.0%
TGCCATGGATCTATCATATC+TGG - chr5.1:57224770-57224789 None:intergenic 40.0%
! ACAGCATTTGAAACAGCACA+TGG + chr5.1:57224824-57224843 MS.gene91239:CDS 40.0%
! ACGGTGTTAGACAAAGAGAA+TGG + chr5.1:57225900-57225919 MS.gene91239:CDS 40.0%
! CAGCATTTGAAACAGCACAT+GGG + chr5.1:57224825-57224844 MS.gene91239:CDS 40.0%
! CATGTTGTTCATGGTTTGCA+AGG + chr5.1:57225073-57225092 MS.gene91239:CDS 40.0%
! GTTGAGTCATTGGTTGATGT+TGG + chr5.1:57224974-57224993 MS.gene91239:CDS 40.0%
! TTGTTGGTTGAGAAGTGCAA+GGG + chr5.1:57224938-57224957 MS.gene91239:CDS 40.0%
! TTTGGCAAGAGTTTTTGCCA+TGG - chr5.1:57225014-57225033 None:intergenic 40.0%
! TTTGTTGGTTGAGAAGTGCA+AGG + chr5.1:57224937-57224956 MS.gene91239:CDS 40.0%
!! TTGATGATGGTAGTATTCGC+AGG + chr5.1:57225966-57225985 MS.gene91239:CDS 40.0%
ACCATTGTACCACTCATTGG+AGG + chr5.1:57225370-57225389 MS.gene91239:intron 45.0%
CAAGCAGAGAATTGATGCCA+TGG - chr5.1:57224785-57224804 None:intergenic 45.0%
CATGGCATCAATTCTCTGCT+TGG + chr5.1:57224783-57224802 MS.gene91239:CDS 45.0%
GATCTCCCACATGTTGTTCA+TGG + chr5.1:57225064-57225083 MS.gene91239:CDS 45.0%
GGCACATCCCAACTTATGTT+GGG + chr5.1:57225451-57225470 MS.gene91239:intron 45.0%
GTAGTCACTAAAACCAGCCA+AGG - chr5.1:57226030-57226049 None:intergenic 45.0%
TCCTCCAATGAGTGGTACAA+TGG - chr5.1:57225374-57225393 None:intergenic 45.0%
! GAGTCATTGGTTGATGTTGG+AGG + chr5.1:57224977-57224996 MS.gene91239:CDS 45.0%
! TCATTGGTTGATGTTGGAGG+AGG + chr5.1:57224980-57224999 MS.gene91239:CDS 45.0%
!!! AGCACATGGGATGTCGTTTT+GGG + chr5.1:57224838-57224857 MS.gene91239:CDS 45.0%
GGGCACATCCCAACTTATGT+TGG + chr5.1:57225450-57225469 MS.gene91239:intron 50.0%
GGTCTTTGTCCTCCAATGAG+TGG - chr5.1:57225382-57225401 None:intergenic 50.0%
! GGTTGATGTTGGAGGAGGTA+CGG + chr5.1:57224985-57225004 MS.gene91239:CDS 50.0%
! GTTGATGTTGGAGGAGGTAC+GGG + chr5.1:57224986-57225005 MS.gene91239:CDS 50.0%
! TTGATGTTGGAGGAGGTACG+GGG + chr5.1:57224987-57225006 MS.gene91239:CDS 50.0%
!! ACATGTGGTGCCACACTCAT+TGG - chr5.1:57224731-57224750 None:intergenic 50.0%
!! CAGCACATGGGATGTCGTTT+TGG + chr5.1:57224837-57224856 MS.gene91239:CDS 50.0%
AGTGTGGCACCACATGTGCA+CGG + chr5.1:57224734-57224753 MS.gene91239:CDS 55.0%
CTGAGTATCCCGTGCACATG+TGG - chr5.1:57224746-57224765 None:intergenic 55.0%
GTGTGGCACCACATGTGCAC+GGG + chr5.1:57224735-57224754 MS.gene91239:CDS 60.0%
! TGGAGGAGGTACGGGGACCA+TGG + chr5.1:57224994-57225013 MS.gene91239:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 57224626 57226100 57224626 ID=MS.gene91239
chr5.1 mRNA 57224626 57226100 57224626 ID=MS.gene91239.t1;Parent=MS.gene91239
chr5.1 exon 57224626 57225168 57224626 ID=MS.gene91239.t1.exon1;Parent=MS.gene91239.t1
chr5.1 CDS 57224626 57225168 57224626 ID=cds.MS.gene91239.t1;Parent=MS.gene91239.t1
chr5.1 exon 57225798 57226100 57225798 ID=MS.gene91239.t1.exon2;Parent=MS.gene91239.t1
chr5.1 CDS 57225798 57226100 57225798 ID=cds.MS.gene91239.t1;Parent=MS.gene91239.t1
Gene Sequence

>MS.gene91239

ATGACTTATTCCGGCTTCTTCTCTCAACAAAATATTACTGAGAATGAGCTAGAGATTGAGTACATGTTAACTGATGTATCTCTATTACTACTTAAGAACAGTCCAATGAGTGTGGCACCACATGTGCACGGGATACTCAGTCCAGATATGATAGATCCATGGCATCAATTCTCTGCTTGGTTAAAAAATGATGATATTACAGCATTTGAAACAGCACATGGGATGTCGTTTTGGGATTATCTTGCACGTGACTCCAAAATTAACAACTCATTTAACGAATCAATGGCAAAAGATACTCGATTAGTTTCCGATTTGTTGGTTGAGAAGTGCAAGGGAGTGTTCCATGAAGTTGAGTCATTGGTTGATGTTGGAGGAGGTACGGGGACCATGGCAAAAACTCTTGCCAAATCATTCCCACAAATGGAGTGCATTGTGTTTGATCTCCCACATGTTGTTCATGGTTTGCAAGGAATTGAAAACTTAAACTATGTTGCTGGAGACATGTTTAAAGAAATTCCGTCAACAGATGCAATTTTGTTGAAGGTAAAATGTTATAATATGCATTTATATATATTACTTGTAATTTTATTGTTTAGAGAAACTCCATAAAAAATACTCTAATGTTCAAAAAACTTGTACTACCATTAAGAATATATATGTTTGTGGGTTTAATGGAAATCTAAAATGATATTGATTTATTATCCATAGAGATAGAGGAAAACAATCAACTAATAGTCCAATCATACCATTGTACCACTCATTGGAGGACAAAGACCATTTTGCTTCTACTTTCATCTGCTAGCTCTTGCTACTAGAAAAGAGTAGGGCACATCCCAACTTATGTTGGGAATGCATGAAGTTACACCCCCATGAGAACAATTATATATATATTTTATCTCAATTTCAACATAGATTATGTTTCTTGAACCAAAACTATGTTTGCCTAAATTATGTCACCATGGTAAAAAAATTAGTTTACACATTTTAGGGTTAATTTGATTTTTTTTTCTTGGTACAAAAGAGGGGATAATTTGTTTTTGTTCACCATATTTTTCTACTAATAACGGCCAATTAGATAATATTTTCCTCGTTTTCTATTTTTTCCACTTTAGTTTGTTCTCTTTTCTCTATCCTTCTTTCGTAACTCAAACATAGTCTTGATGAAATTACAGTCGATATTGCATGATTGGAATGACGAGGAATGTGTAAAGATATTAAAGAATTGCAAGGATGCAATATCAAAGAAAGGTAAAGGGAAGGTGGTTATCATTGACACGGTGTTAGACAAAGAGAATGGAAACATTAATGAATCAGTTGAAACACAACTCTTCTATGACATGTTGATGATGGTAGTATTCGCAGGAAAAGAGAGAAACGAGAAAGAATGGATTAAGTTGATTTCCTTGGCTGGTTTTAGTGACTACAAGATTACTCCAATTTTAGGATCAAGGTCTATGATTGAGATCTATCCATGA
Protein sequence

>MS.gene91239.t1

MTYSGFFSQQNITENELEIEYMLTDVSLLLLKNSPMSVAPHVHGILSPDMIDPWHQFSAWLKNDDITAFETAHGMSFWDYLARDSKINNSFNESMAKDTRLVSDLLVEKCKGVFHEVESLVDVGGGTGTMAKTLAKSFPQMECIVFDLPHVVHGLQGIENLNYVAGDMFKEIPSTDAILLKSILHDWNDEECVKILKNCKDAISKKGKGKVVIIDTVLDKENGNINESVETQLFYDMLMMVVFAGKERNEKEWIKLISLAGFSDYKITPILGSRSMIEIYP