Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91299.t1 | XP_003592478.1 | 87.7 | 211 | 9 | 3 | 1 | 211 | 1 | 194 | 4.90E-94 | 354 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91299.t1 | Q9SN23 | 68.6 | 118 | 35 | 1 | 1 | 118 | 1 | 116 | 5.7e-43 | 175.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91299.t1 | G7IE59 | 87.7 | 211 | 9 | 3 | 1 | 211 | 1 | 194 | 3.5e-94 | 354.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene91299.t1 | TF | LOB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060276 | MS.gene91299 | 0.880869 | 3.85E-70 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91299.t1 | MTR_1g106420 | 87.678 | 211 | 9 | 3 | 1 | 211 | 1 | 194 | 1.09e-124 | 350 |
| MS.gene91299.t1 | MTR_5g015880 | 65.873 | 126 | 40 | 2 | 1 | 126 | 1 | 123 | 4.07e-55 | 175 |
| MS.gene91299.t1 | MTR_4g095600 | 70.085 | 117 | 33 | 1 | 1 | 117 | 1 | 115 | 1.42e-54 | 174 |
| MS.gene91299.t1 | MTR_7g007010 | 61.538 | 104 | 40 | 0 | 1 | 104 | 3 | 106 | 2.13e-46 | 155 |
| MS.gene91299.t1 | MTR_6g018270 | 44.720 | 161 | 84 | 2 | 1 | 161 | 3 | 158 | 3.89e-45 | 151 |
| MS.gene91299.t1 | MTR_6g453130 | 52.727 | 110 | 42 | 2 | 1 | 104 | 3 | 108 | 1.08e-37 | 130 |
| MS.gene91299.t1 | MTR_1g110840 | 39.157 | 166 | 84 | 5 | 1 | 157 | 3 | 160 | 4.25e-37 | 129 |
| MS.gene91299.t1 | MTR_6g054150 | 49.057 | 106 | 52 | 1 | 1 | 104 | 3 | 108 | 9.24e-35 | 123 |
| MS.gene91299.t1 | MTR_6g054080 | 41.732 | 127 | 67 | 2 | 1 | 125 | 3 | 124 | 2.88e-33 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91299.t1 | AT3G49940 | 68.644 | 118 | 35 | 1 | 1 | 118 | 1 | 116 | 2.02e-54 | 174 |
| MS.gene91299.t1 | AT5G67420 | 37.698 | 252 | 114 | 4 | 1 | 211 | 1 | 250 | 6.60e-52 | 168 |
| MS.gene91299.t1 | AT5G67420 | 37.698 | 252 | 114 | 4 | 1 | 211 | 1 | 250 | 6.60e-52 | 168 |
| MS.gene91299.t1 | AT4G37540 | 60.741 | 135 | 44 | 3 | 1 | 129 | 1 | 132 | 1.24e-50 | 164 |
| MS.gene91299.t1 | AT3G02550 | 61.538 | 104 | 40 | 0 | 1 | 104 | 3 | 106 | 1.17e-46 | 155 |
| MS.gene91299.t1 | AT1G67100 | 60.000 | 105 | 42 | 0 | 1 | 105 | 3 | 107 | 4.76e-44 | 147 |
| MS.gene91299.t1 | AT1G68510 | 48.462 | 130 | 65 | 1 | 1 | 130 | 3 | 130 | 1.01e-41 | 141 |
Find 53 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAACAGCAAGAGTTTATTT+TGG | 0.186602 | 1.3:+73086365 | MS.gene91299:CDS |
| AGCCCAACTGCTCCATTTAC+TGG | 0.215944 | 1.3:-73086204 | None:intergenic |
| TTTATGACATACATGATCTT+TGG | 0.296751 | 1.3:-73085984 | None:intergenic |
| AGAACTATAAATCCAGTAAA+TGG | 0.325401 | 1.3:+73086192 | MS.gene91299:CDS |
| ATGGAGCAGTTGGGCTGTTA+TGG | 0.333353 | 1.3:+73086211 | MS.gene91299:CDS |
| GTCTTTGTCACCAAGTTCTT+CGG | 0.371969 | 1.3:+73085919 | MS.gene91299:CDS |
| TGTAATGAAGAAGCACAATC+TGG | 0.379276 | 1.3:+73086396 | MS.gene91299:CDS |
| GATCTTTGGTTGGGAGGAAC+AGG | 0.395798 | 1.3:-73085970 | None:intergenic |
| GACATACATGATCTTTGGTT+GGG | 0.427328 | 1.3:-73085979 | None:intergenic |
| CACTTATGTATGAAGCTGTT+GGG | 0.452221 | 1.3:+73086169 | MS.gene91299:CDS |
| TGGCGTTAGCCTGTGCTTGT+GGG | 0.452316 | 1.3:-73085894 | None:intergenic |
| GACACATGAGTCTGAGGAAT+CGG | 0.458754 | 1.3:+73086485 | MS.gene91299:CDS |
| TCCACACCTAACAACTGATC+AGG | 0.464754 | 1.3:-73086309 | None:intergenic |
| GGTGGCACGGCCGAAGAACT+TGG | 0.467396 | 1.3:-73085929 | None:intergenic |
| AATCCAGTAAATGGAGCAGT+TGG | 0.467406 | 1.3:+73086201 | MS.gene91299:CDS |
| GAATCGGAGTCGTCCACGTT+GGG | 0.467550 | 1.3:+73086501 | MS.gene91299:CDS |
| GCACTCCCTGATCAGTTGTT+AGG | 0.477941 | 1.3:+73086303 | MS.gene91299:CDS |
| GTGGCGTTAGCCTGTGCTTG+TGG | 0.478831 | 1.3:-73085895 | None:intergenic |
| TGACATACATGATCTTTGGT+TGG | 0.489850 | 1.3:-73085980 | None:intergenic |
| CCCTGATCAGTTGTTAGGTG+TGG | 0.497837 | 1.3:+73086308 | MS.gene91299:CDS |
| CCAAAATAAACTCTTGCTGT+TGG | 0.526318 | 1.3:-73086365 | None:intergenic |
| ATCCAGTAAATGGAGCAGTT+GGG | 0.536591 | 1.3:+73086202 | MS.gene91299:CDS |
| TCACTTATGTATGAAGCTGT+TGG | 0.539852 | 1.3:+73086168 | MS.gene91299:CDS |
| TGCATCCCTTTCTCAAAACT+CGG | 0.542491 | 1.3:-73085828 | None:intergenic |
| GAACAAGTGCTGAGAGGTAA+CGG | 0.548928 | 1.3:+73086267 | MS.gene91299:CDS |
| GAGATCATAAGAATAGTGTA+AGG | 0.555999 | 1.3:-73086590 | None:intergenic |
| GATACAAAACCCACAAGCAC+AGG | 0.556238 | 1.3:+73085885 | MS.gene91299:CDS |
| TATAATAAAATGAGTTGCAA+CGG | 0.559441 | 1.3:+73085802 | None:intergenic |
| TTTGAGAAAGGGATGCAGTG+AGG | 0.560200 | 1.3:+73085834 | MS.gene91299:CDS |
| ATAAGAATAGTGTAAGGAGT+TGG | 0.568732 | 1.3:-73086584 | None:intergenic |
| CAGCTTCATACATAAGTGAC+TGG | 0.569517 | 1.3:-73086164 | None:intergenic |
| GTAATGAAGAAGCACAATCT+GGG | 0.578649 | 1.3:+73086397 | MS.gene91299:CDS |
| ATGCTACTTCTCAAACTCAA+AGG | 0.580159 | 1.3:+73086559 | MS.gene91299:CDS |
| ATACATGATCTTTGGTTGGG+AGG | 0.585857 | 1.3:-73085976 | None:intergenic |
| AGTACTCTAGAGTGGTGAGA+AGG | 0.591970 | 1.3:+73086430 | MS.gene91299:CDS |
| GGCTGTTATGGACTGGAAAG+TGG | 0.603769 | 1.3:+73086223 | MS.gene91299:CDS |
| GAAAAGACATGAGGGTGGCA+CGG | 0.613200 | 1.3:-73085942 | None:intergenic |
| GCAGTTGGGCTGTTATGGAC+TGG | 0.615948 | 1.3:+73086216 | MS.gene91299:CDS |
| CCACACCTAACAACTGATCA+GGG | 0.634962 | 1.3:-73086308 | None:intergenic |
| GATTTCACAGTACTCTAGAG+TGG | 0.637833 | 1.3:+73086422 | MS.gene91299:CDS |
| TGCGGAGACACATGAGTCTG+AGG | 0.638167 | 1.3:+73086479 | MS.gene91299:CDS |
| GGAATCGGAGTCGTCCACGT+TGG | 0.646426 | 1.3:+73086500 | MS.gene91299:CDS |
| AAGTGGCAATTCTGTCAACT+TGG | 0.652925 | 1.3:+73086240 | MS.gene91299:CDS |
| CCAAGCAGAAAAGCAACGTG+CGG | 0.655633 | 1.3:+73086461 | MS.gene91299:CDS |
| TTCTGTTCTACTTCCCAACG+TGG | 0.659625 | 1.3:-73086514 | None:intergenic |
| CAGGTGAAAGAAAAGACATG+AGG | 0.660626 | 1.3:-73085951 | None:intergenic |
| AGGTGAAAGAAAAGACATGA+GGG | 0.666800 | 1.3:-73085950 | None:intergenic |
| TAACAACTGATCAGGGAGTG+CGG | 0.677605 | 1.3:-73086301 | None:intergenic |
| TGCTACGAGATTGTCTAACG+TGG | 0.685370 | 1.3:+73085863 | MS.gene91299:CDS |
| GAACTTGGTGACAAAGACAG+TGG | 0.712149 | 1.3:-73085914 | None:intergenic |
| TGAAAGAAAAGACATGAGGG+TGG | 0.725920 | 1.3:-73085947 | None:intergenic |
| GGTGTTGAACAAGTGCTGAG+AGG | 0.756300 | 1.3:+73086261 | MS.gene91299:CDS |
| CAAGTGCTGAGAGGTAACGG+CGG | 0.757236 | 1.3:+73086270 | MS.gene91299:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTAGTTTGAATATGTATT+TGG | - | chr1.3:73086052-73086071 | None:intergenic | 15.0% |
| !! | ATTCAATTACTCTATATTAC+AGG | + | chr1.3:73086135-73086154 | MS.gene91299:intron | 20.0% |
| !!! | TCAAACTAATTTGTATGTAT+AGG | + | chr1.3:73086061-73086080 | MS.gene91299:intron | 20.0% |
| ! | AGAACTATAAATCCAGTAAA+TGG | + | chr1.3:73086192-73086211 | MS.gene91299:CDS | 25.0% |
| ! | TTTATGACATACATGATCTT+TGG | - | chr1.3:73085987-73086006 | None:intergenic | 25.0% |
| ATAAGAATAGTGTAAGGAGT+TGG | - | chr1.3:73086587-73086606 | None:intergenic | 30.0% | |
| !! | CAACAGCAAGAGTTTATTTT+GGG | + | chr1.3:73086366-73086385 | MS.gene91299:CDS | 30.0% |
| AGGTGAAAGAAAAGACATGA+GGG | - | chr1.3:73085953-73085972 | None:intergenic | 35.0% | |
| CACTTATGTATGAAGCTGTT+GGG | + | chr1.3:73086169-73086188 | MS.gene91299:CDS | 35.0% | |
| GACATACATGATCTTTGGTT+GGG | - | chr1.3:73085982-73086001 | None:intergenic | 35.0% | |
| TCACTTATGTATGAAGCTGT+TGG | + | chr1.3:73086168-73086187 | MS.gene91299:CDS | 35.0% | |
| TGACATACATGATCTTTGGT+TGG | - | chr1.3:73085983-73086002 | None:intergenic | 35.0% | |
| ! | ATGCTACTTCTCAAACTCAA+AGG | + | chr1.3:73086559-73086578 | MS.gene91299:CDS | 35.0% |
| ! | CCAAAATAAACTCTTGCTGT+TGG | - | chr1.3:73086368-73086387 | None:intergenic | 35.0% |
| ! | CCAACAGCAAGAGTTTATTT+TGG | + | chr1.3:73086365-73086384 | MS.gene91299:CDS | 35.0% |
| ! | GTAATGAAGAAGCACAATCT+GGG | + | chr1.3:73086397-73086416 | MS.gene91299:CDS | 35.0% |
| ! | TGTAATGAAGAAGCACAATC+TGG | + | chr1.3:73086396-73086415 | MS.gene91299:CDS | 35.0% |
| AAGTGGCAATTCTGTCAACT+TGG | + | chr1.3:73086240-73086259 | MS.gene91299:CDS | 40.0% | |
| AATCCAGTAAATGGAGCAGT+TGG | + | chr1.3:73086201-73086220 | MS.gene91299:CDS | 40.0% | |
| ATACATGATCTTTGGTTGGG+AGG | - | chr1.3:73085979-73085998 | None:intergenic | 40.0% | |
| ATCCAGTAAATGGAGCAGTT+GGG | + | chr1.3:73086202-73086221 | MS.gene91299:CDS | 40.0% | |
| CAGCTTCATACATAAGTGAC+TGG | - | chr1.3:73086167-73086186 | None:intergenic | 40.0% | |
| CAGGTGAAAGAAAAGACATG+AGG | - | chr1.3:73085954-73085973 | None:intergenic | 40.0% | |
| GTCTTTGTCACCAAGTTCTT+CGG | + | chr1.3:73085919-73085938 | MS.gene91299:CDS | 40.0% | |
| TGAAAGAAAAGACATGAGGG+TGG | - | chr1.3:73085950-73085969 | None:intergenic | 40.0% | |
| TGCATCCCTTTCTCAAAACT+CGG | - | chr1.3:73085831-73085850 | None:intergenic | 40.0% | |
| ! | GATTTCACAGTACTCTAGAG+TGG | + | chr1.3:73086422-73086441 | MS.gene91299:CDS | 40.0% |
| CCACACCTAACAACTGATCA+GGG | - | chr1.3:73086311-73086330 | None:intergenic | 45.0% | |
| GACACATGAGTCTGAGGAAT+CGG | + | chr1.3:73086485-73086504 | MS.gene91299:CDS | 45.0% | |
| GATACAAAACCCACAAGCAC+AGG | + | chr1.3:73085885-73085904 | MS.gene91299:CDS | 45.0% | |
| TAACAACTGATCAGGGAGTG+CGG | - | chr1.3:73086304-73086323 | None:intergenic | 45.0% | |
| TCCACACCTAACAACTGATC+AGG | - | chr1.3:73086312-73086331 | None:intergenic | 45.0% | |
| TGCTACGAGATTGTCTAACG+TGG | + | chr1.3:73085863-73085882 | MS.gene91299:CDS | 45.0% | |
| TTCTGTTCTACTTCCCAACG+TGG | - | chr1.3:73086517-73086536 | None:intergenic | 45.0% | |
| TTTGAGAAAGGGATGCAGTG+AGG | + | chr1.3:73085834-73085853 | MS.gene91299:CDS | 45.0% | |
| ! | GAACTTGGTGACAAAGACAG+TGG | - | chr1.3:73085917-73085936 | None:intergenic | 45.0% |
| !! | AGTACTCTAGAGTGGTGAGA+AGG | + | chr1.3:73086430-73086449 | MS.gene91299:CDS | 45.0% |
| !! | GAACAAGTGCTGAGAGGTAA+CGG | + | chr1.3:73086267-73086286 | MS.gene91299:CDS | 45.0% |
| !!! | GGTTGCCGAGTTTTGAGAAA+GGG | + | chr1.3:73085823-73085842 | MS.gene91299:CDS | 45.0% |
| AGCCCAACTGCTCCATTTAC+TGG | - | chr1.3:73086207-73086226 | None:intergenic | 50.0% | |
| ATGGAGCAGTTGGGCTGTTA+TGG | + | chr1.3:73086211-73086230 | MS.gene91299:CDS | 50.0% | |
| CCAAGCAGAAAAGCAACGTG+CGG | + | chr1.3:73086461-73086480 | MS.gene91299:CDS | 50.0% | |
| CCCTGATCAGTTGTTAGGTG+TGG | + | chr1.3:73086308-73086327 | MS.gene91299:CDS | 50.0% | |
| GAAAAGACATGAGGGTGGCA+CGG | - | chr1.3:73085945-73085964 | None:intergenic | 50.0% | |
| GATCTTTGGTTGGGAGGAAC+AGG | - | chr1.3:73085973-73085992 | None:intergenic | 50.0% | |
| GCACTCCCTGATCAGTTGTT+AGG | + | chr1.3:73086303-73086322 | MS.gene91299:CDS | 50.0% | |
| !! | GGCTGTTATGGACTGGAAAG+TGG | + | chr1.3:73086223-73086242 | MS.gene91299:CDS | 50.0% |
| !! | GGTGTTGAACAAGTGCTGAG+AGG | + | chr1.3:73086261-73086280 | MS.gene91299:CDS | 50.0% |
| !!! | CGGTTGCCGAGTTTTGAGAA+AGG | + | chr1.3:73085822-73085841 | MS.gene91299:CDS | 50.0% |
| GAATCGGAGTCGTCCACGTT+GGG | + | chr1.3:73086501-73086520 | MS.gene91299:CDS | 55.0% | |
| GCAGTTGGGCTGTTATGGAC+TGG | + | chr1.3:73086216-73086235 | MS.gene91299:CDS | 55.0% | |
| TGCGGAGACACATGAGTCTG+AGG | + | chr1.3:73086479-73086498 | MS.gene91299:CDS | 55.0% | |
| ! | TGGCGTTAGCCTGTGCTTGT+GGG | - | chr1.3:73085897-73085916 | None:intergenic | 55.0% |
| !! | CAAGTGCTGAGAGGTAACGG+CGG | + | chr1.3:73086270-73086289 | MS.gene91299:CDS | 55.0% |
| !!! | CCGCACGTTGCTTTTCTGCT+TGG | - | chr1.3:73086464-73086483 | None:intergenic | 55.0% |
| GGAATCGGAGTCGTCCACGT+TGG | + | chr1.3:73086500-73086519 | MS.gene91299:CDS | 60.0% | |
| ! | GTGGCGTTAGCCTGTGCTTG+TGG | - | chr1.3:73085898-73085917 | None:intergenic | 60.0% |
| GGTGGCACGGCCGAAGAACT+TGG | - | chr1.3:73085932-73085951 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 73085811 | 73086608 | 73085811 | ID=MS.gene91299 |
| chr1.3 | mRNA | 73085811 | 73086608 | 73085811 | ID=MS.gene91299.t1;Parent=MS.gene91299 |
| chr1.3 | exon | 73085811 | 73085994 | 73085811 | ID=MS.gene91299.t1.exon1;Parent=MS.gene91299.t1 |
| chr1.3 | CDS | 73085811 | 73085994 | 73085811 | ID=cds.MS.gene91299.t1;Parent=MS.gene91299.t1 |
| chr1.3 | exon | 73086157 | 73086608 | 73086157 | ID=MS.gene91299.t1.exon2;Parent=MS.gene91299.t1 |
| chr1.3 | CDS | 73086157 | 73086608 | 73086157 | ID=cds.MS.gene91299.t1;Parent=MS.gene91299.t1 |
| Gene Sequence |
| Protein sequence |
