Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91410.t1 | XP_013469869.1 | 94.5 | 199 | 11 | 0 | 1 | 199 | 1 | 199 | 3.90E-101 | 377.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91410.t1 | Q84MC0 | 53.7 | 203 | 86 | 3 | 1 | 199 | 1 | 199 | 3.0e-54 | 213.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91410.t1 | A0A072VQ56 | 94.5 | 199 | 11 | 0 | 1 | 199 | 1 | 199 | 2.8e-101 | 377.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050649 | MS.gene91410 | 0.828872 | 7.06E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91410.t1 | MTR_1g105305 | 94.472 | 199 | 11 | 0 | 1 | 199 | 1 | 199 | 2.65e-137 | 382 |
| MS.gene91410.t1 | MTR_5g005710 | 63.902 | 205 | 64 | 2 | 1 | 199 | 1 | 201 | 6.58e-96 | 277 |
| MS.gene91410.t1 | MTR_1g082770 | 36.943 | 157 | 96 | 3 | 20 | 173 | 20 | 176 | 1.02e-31 | 114 |
| MS.gene91410.t1 | MTR_7g113640 | 38.889 | 144 | 84 | 3 | 19 | 159 | 17 | 159 | 4.14e-31 | 112 |
| MS.gene91410.t1 | MTR_1g082830 | 36.170 | 141 | 88 | 2 | 31 | 169 | 47 | 187 | 1.55e-26 | 101 |
| MS.gene91410.t1 | MTR_1g082790 | 34.783 | 115 | 74 | 1 | 31 | 144 | 46 | 160 | 1.47e-18 | 79.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91410.t1 | AT3G06035 | 58.659 | 179 | 70 | 2 | 25 | 199 | 21 | 199 | 3.16e-72 | 217 |
| MS.gene91410.t1 | AT5G19250 | 61.078 | 167 | 63 | 1 | 24 | 188 | 19 | 185 | 1.85e-68 | 207 |
| MS.gene91410.t1 | AT1G54860 | 41.808 | 177 | 85 | 4 | 1 | 168 | 2 | 169 | 1.02e-46 | 152 |
| MS.gene91410.t1 | AT5G19240 | 45.349 | 172 | 88 | 3 | 23 | 192 | 20 | 187 | 2.16e-46 | 152 |
| MS.gene91410.t1 | AT5G19230 | 45.562 | 169 | 80 | 3 | 1 | 169 | 1 | 157 | 3.90e-41 | 138 |
| MS.gene91410.t1 | AT5G19230 | 45.562 | 169 | 80 | 3 | 1 | 169 | 1 | 157 | 3.90e-41 | 138 |
Find 42 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAATTGACATTTAGCTAAA+AGG | 0.190847 | 1.3:+71808848 | None:intergenic |
| CCAAGTTCTCTCTATTCTTC+TGG | 0.209568 | 1.3:-71809647 | MS.gene91410:CDS |
| GTTATGCCTGCCTGTGTTCC+TGG | 0.264326 | 1.3:-71808799 | MS.gene91410:CDS |
| TCCAACTTTGGAAATCAAAT+TGG | 0.265283 | 1.3:+71808606 | None:intergenic |
| TGAGAGAGATAATCTTTATC+AGG | 0.299934 | 1.3:-71809040 | MS.gene91410:CDS |
| TGACATTTAGCTAAAAGGTT+TGG | 0.301742 | 1.3:+71808853 | None:intergenic |
| AGCTAAAAGGTTTGGGTAGT+TGG | 0.318984 | 1.3:+71808861 | None:intergenic |
| GTATTGGCTCAGAAGATAAC+TGG | 0.333128 | 1.3:-71808693 | MS.gene91410:CDS |
| GACATTTAGCTAAAAGGTTT+GGG | 0.340607 | 1.3:+71808854 | None:intergenic |
| CCAGAAGAATAGAGAGAACT+TGG | 0.347419 | 1.3:+71809647 | None:intergenic |
| GCAATAAATCATTCCAACTT+TGG | 0.358521 | 1.3:+71808594 | None:intergenic |
| TACATGGTTGATTCTTGAAT+TGG | 0.360348 | 1.3:+71808932 | None:intergenic |
| ATTGGCAGCATTAGAACTAT+AGG | 0.374058 | 1.3:+71808624 | None:intergenic |
| GCCAATTTGATTTCCAAAGT+TGG | 0.375408 | 1.3:-71808607 | MS.gene91410:CDS |
| TTGGCAGCATTAGAACTATA+GGG | 0.380106 | 1.3:+71808625 | None:intergenic |
| CATCCTCTAGGTATCATAAC+AGG | 0.398840 | 1.3:+71808542 | None:intergenic |
| TCACCGTCATCACATTTAAC+TGG | 0.400596 | 1.3:+71809585 | None:intergenic |
| GACACACAGTATACAGGAAT+TGG | 0.421140 | 1.3:-71808715 | MS.gene91410:CDS |
| TGCTCAATTCTCTTGTTAGC+TGG | 0.427277 | 1.3:-71808574 | MS.gene91410:CDS |
| CTCAACGACACACAGTATAC+AGG | 0.481619 | 1.3:-71808721 | MS.gene91410:CDS |
| TTGACCACTGACACTCCAAC+AGG | 0.483770 | 1.3:-71808658 | MS.gene91410:CDS |
| TTTGGGTAGTTGGAGAATTG+AGG | 0.485525 | 1.3:+71808871 | None:intergenic |
| ATTGGCTATTTCTGATACAA+AGG | 0.497292 | 1.3:-71808830 | MS.gene91410:CDS |
| ACGAGGCTTGAAGCTAGGCC+AGG | 0.504069 | 1.3:+71808781 | None:intergenic |
| TGAAGCTAGGCCAGGAACAC+AGG | 0.509872 | 1.3:+71808789 | None:intergenic |
| GCTAGGCCAGGAACACAGGC+AGG | 0.513127 | 1.3:+71808793 | None:intergenic |
| GAGAATTGAGGTTCAGTACC+TGG | 0.522201 | 1.3:+71808883 | None:intergenic |
| GCTATTTCTGATACAAAGGA+TGG | 0.531181 | 1.3:-71808826 | MS.gene91410:CDS |
| CAGTATACAGGAATTGGTAT+TGG | 0.536270 | 1.3:-71808709 | MS.gene91410:CDS |
| GCACCTGTGGTATTTGTACA+TGG | 0.542966 | 1.3:+71808916 | None:intergenic |
| TCGTGAAGTTAGTGAGGACG+AGG | 0.562843 | 1.3:+71808764 | None:intergenic |
| TGAGGACGAGGCTTGAAGCT+AGG | 0.566605 | 1.3:+71808776 | None:intergenic |
| GTTTCCTGTTGGAGTGTCAG+TGG | 0.576612 | 1.3:+71808654 | None:intergenic |
| GAGATTTCGTGAAGTTAGTG+AGG | 0.582648 | 1.3:+71808758 | None:intergenic |
| GAGAGAGATAATCTTTATCA+GGG | 0.592178 | 1.3:-71809039 | MS.gene91410:CDS |
| TATCCAGTTAAATGTGATGA+CGG | 0.600716 | 1.3:-71809588 | MS.gene91410:intron |
| TGGTACTGTATTAGCACCTG+TGG | 0.607702 | 1.3:+71808903 | None:intergenic |
| TGGCAGCATTAGAACTATAG+GGG | 0.612217 | 1.3:+71808626 | None:intergenic |
| ACAGGTGCTAATACAGTACC+AGG | 0.621692 | 1.3:-71808901 | MS.gene91410:CDS |
| CAGAAGAATAGAGAGAACTT+GGG | 0.624874 | 1.3:+71809648 | None:intergenic |
| CAACCATGTACAAATACCAC+AGG | 0.631778 | 1.3:-71808919 | MS.gene91410:CDS |
| GGCAGCATTAGAACTATAGG+GGG | 0.666389 | 1.3:+71808627 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTGACCAATACAAAATA+TGG | + | chr1.3:71808708-71808727 | None:intergenic | 20.0% |
| ! | ATTCATGAGATTAATGTCAT+TGG | - | chr1.3:71808773-71808792 | MS.gene91410:CDS | 25.0% |
| !! | TCTTAGACCATATTTTGTAT+TGG | - | chr1.3:71808698-71808717 | MS.gene91410:CDS | 25.0% |
| CCAATTGACATTTAGCTAAA+AGG | + | chr1.3:71809367-71809386 | None:intergenic | 30.0% | |
| GACATTTAGCTAAAAGGTTT+GGG | + | chr1.3:71809361-71809380 | None:intergenic | 30.0% | |
| GAGAGAGATAATCTTTATCA+GGG | - | chr1.3:71809173-71809192 | MS.gene91410:intron | 30.0% | |
| GCAATAAATCATTCCAACTT+TGG | + | chr1.3:71809621-71809640 | None:intergenic | 30.0% | |
| TATCCAGTTAAATGTGATGA+CGG | - | chr1.3:71808624-71808643 | MS.gene91410:CDS | 30.0% | |
| TGACATTTAGCTAAAAGGTT+TGG | + | chr1.3:71809362-71809381 | None:intergenic | 30.0% | |
| TGAGAGAGATAATCTTTATC+AGG | - | chr1.3:71809172-71809191 | MS.gene91410:intron | 30.0% | |
| TGTCAGTGAACAAAATGATT+TGG | + | chr1.3:71809067-71809086 | None:intergenic | 30.0% | |
| TTTCAGCTTCATTGTGTATA+TGG | + | chr1.3:71809133-71809152 | None:intergenic | 30.0% | |
| ! | TACATGGTTGATTCTTGAAT+TGG | + | chr1.3:71809283-71809302 | None:intergenic | 30.0% |
| ! | TCCAACTTTGGAAATCAAAT+TGG | + | chr1.3:71809609-71809628 | None:intergenic | 30.0% |
| !! | ATTGGCTATTTCTGATACAA+AGG | - | chr1.3:71809382-71809401 | MS.gene91410:intron | 30.0% |
| !! | CCTTTTAGCTAAATGTCAAT+TGG | - | chr1.3:71809364-71809383 | MS.gene91410:intron | 30.0% |
| ATGCCTCTTAAAACATTCAC+TGG | - | chr1.3:71808966-71808985 | MS.gene91410:CDS | 35.0% | |
| ATTGGCAGCATTAGAACTAT+AGG | + | chr1.3:71809591-71809610 | None:intergenic | 35.0% | |
| CAGAAGAATAGAGAGAACTT+GGG | + | chr1.3:71808567-71808586 | None:intergenic | 35.0% | |
| CAGTATACAGGAATTGGTAT+TGG | - | chr1.3:71809503-71809522 | MS.gene91410:intron | 35.0% | |
| CTAAGCTTGAAATGAGTCAT+GGG | + | chr1.3:71808846-71808865 | None:intergenic | 35.0% | |
| TTGGCAGCATTAGAACTATA+GGG | + | chr1.3:71809590-71809609 | None:intergenic | 35.0% | |
| ! | GCCAATTTGATTTCCAAAGT+TGG | - | chr1.3:71809605-71809624 | MS.gene91410:CDS | 35.0% |
| !! | GCTATTTCTGATACAAAGGA+TGG | - | chr1.3:71809386-71809405 | MS.gene91410:intron | 35.0% |
| !! | TGTCCAGTGAATGTTTTAAG+AGG | + | chr1.3:71808972-71808991 | None:intergenic | 35.0% |
| AGCTAAAAGGTTTGGGTAGT+TGG | + | chr1.3:71809354-71809373 | None:intergenic | 40.0% | |
| CAACCATGTACAAATACCAC+AGG | - | chr1.3:71809293-71809312 | MS.gene91410:intron | 40.0% | |
| CCAAGTTCTCTCTATTCTTC+TGG | - | chr1.3:71808565-71808584 | MS.gene91410:CDS | 40.0% | |
| CCAGAAGAATAGAGAGAACT+TGG | + | chr1.3:71808568-71808587 | None:intergenic | 40.0% | |
| CTCATCTGCAGAAAAAACAC+TGG | + | chr1.3:71809156-71809175 | None:intergenic | 40.0% | |
| GACACACAGTATACAGGAAT+TGG | - | chr1.3:71809497-71809516 | MS.gene91410:intron | 40.0% | |
| GAGATTTCGTGAAGTTAGTG+AGG | + | chr1.3:71809457-71809476 | None:intergenic | 40.0% | |
| GCTAAGCTTGAAATGAGTCA+TGG | + | chr1.3:71808847-71808866 | None:intergenic | 40.0% | |
| TCACCGTCATCACATTTAAC+TGG | + | chr1.3:71808630-71808649 | None:intergenic | 40.0% | |
| TGCTCAATTCTCTTGTTAGC+TGG | - | chr1.3:71809638-71809657 | MS.gene91410:CDS | 40.0% | |
| TGGCAGCATTAGAACTATAG+GGG | + | chr1.3:71809589-71809608 | None:intergenic | 40.0% | |
| TTGAGTAATGCACAGATGAC+AGG | + | chr1.3:71808997-71809016 | None:intergenic | 40.0% | |
| TTTGGGTAGTTGGAGAATTG+AGG | + | chr1.3:71809344-71809363 | None:intergenic | 40.0% | |
| ! | TCTCAGTTGTGACCTTTTTC+TGG | - | chr1.3:71809088-71809107 | MS.gene91410:intron | 40.0% |
| !! | GGTCAGCTATTTTATCAGCA+AGG | + | chr1.3:71809262-71809281 | None:intergenic | 40.0% |
| !! | GTATTGGCTCAGAAGATAAC+TGG | - | chr1.3:71809519-71809538 | MS.gene91410:intron | 40.0% |
| ACAGGTGCTAATACAGTACC+AGG | - | chr1.3:71809311-71809330 | MS.gene91410:intron | 45.0% | |
| CTCAACGACACACAGTATAC+AGG | - | chr1.3:71809491-71809510 | MS.gene91410:intron | 45.0% | |
| GAGAATTGAGGTTCAGTACC+TGG | + | chr1.3:71809332-71809351 | None:intergenic | 45.0% | |
| GCACCTGTGGTATTTGTACA+TGG | + | chr1.3:71809299-71809318 | None:intergenic | 45.0% | |
| GGCAGCATTAGAACTATAGG+GGG | + | chr1.3:71809588-71809607 | None:intergenic | 45.0% | |
| TGGTACTGTATTAGCACCTG+TGG | + | chr1.3:71809312-71809331 | None:intergenic | 45.0% | |
| TGTGCATGTGTGCCAGAAAA+AGG | + | chr1.3:71809103-71809122 | None:intergenic | 45.0% | |
| TAGGGGGCAAAGTTTCCTGT+TGG | + | chr1.3:71809572-71809591 | None:intergenic | 50.0% | |
| TCATCGCAGTCGCGAGATTA+GGG | + | chr1.3:71808920-71808939 | None:intergenic | 50.0% | |
| TCGTGAAGTTAGTGAGGACG+AGG | + | chr1.3:71809451-71809470 | None:intergenic | 50.0% | |
| TTCATCGCAGTCGCGAGATT+AGG | + | chr1.3:71808921-71808940 | None:intergenic | 50.0% | |
| TTGACCACTGACACTCCAAC+AGG | - | chr1.3:71809554-71809573 | MS.gene91410:intron | 50.0% | |
| ! | GTTTCCTGTTGGAGTGTCAG+TGG | + | chr1.3:71809561-71809580 | None:intergenic | 50.0% |
| GTTATGCCTGCCTGTGTTCC+TGG | - | chr1.3:71809413-71809432 | MS.gene91410:intron | 55.0% | |
| TGAAGCTAGGCCAGGAACAC+AGG | + | chr1.3:71809426-71809445 | None:intergenic | 55.0% | |
| TGAGGACGAGGCTTGAAGCT+AGG | + | chr1.3:71809439-71809458 | None:intergenic | 55.0% | |
| ACGAGGCTTGAAGCTAGGCC+AGG | + | chr1.3:71809434-71809453 | None:intergenic | 60.0% | |
| GCTAGGCCAGGAACACAGGC+AGG | + | chr1.3:71809422-71809441 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 71808555 | 71809679 | 71808555 | ID=MS.gene91410 |
| chr1.3 | mRNA | 71808555 | 71809679 | 71808555 | ID=MS.gene91410.t1;Parent=MS.gene91410 |
| chr1.3 | exon | 71809589 | 71809679 | 71809589 | ID=MS.gene91410.t1.exon1;Parent=MS.gene91410.t1 |
| chr1.3 | CDS | 71809589 | 71809679 | 71809589 | ID=cds.MS.gene91410.t1;Parent=MS.gene91410.t1 |
| chr1.3 | exon | 71808555 | 71809063 | 71808555 | ID=MS.gene91410.t1.exon2;Parent=MS.gene91410.t1 |
| chr1.3 | CDS | 71808555 | 71809063 | 71808555 | ID=cds.MS.gene91410.t1;Parent=MS.gene91410.t1 |
| Gene Sequence |
| Protein sequence |
