Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91566.t1 | AFQ94047.1 | 92.5 | 146 | 3 | 1 | 1 | 146 | 14 | 151 | 1.60E-67 | 265.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91566.t1 | Q9SR34 | 36.5 | 178 | 67 | 7 | 1 | 139 | 1 | 171 | 7.3e-13 | 75.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91566.t1 | J7K954 | 92.5 | 146 | 3 | 1 | 1 | 146 | 14 | 151 | 1.1e-67 | 265.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene91566.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057406 | MS.gene91566 | 0.814553 | 1.45E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91566.t1 | MTR_7g063220 | 91.781 | 146 | 4 | 1 | 1 | 146 | 1 | 138 | 3.32e-96 | 273 |
| MS.gene91566.t1 | MTR_4g064873 | 38.562 | 153 | 64 | 3 | 24 | 146 | 21 | 173 | 1.80e-22 | 87.8 |
| MS.gene91566.t1 | MTR_1g075430 | 77.500 | 40 | 9 | 0 | 19 | 58 | 13 | 52 | 8.27e-17 | 73.6 |
| MS.gene91566.t1 | MTR_3g068095 | 68.000 | 50 | 16 | 0 | 24 | 73 | 55 | 104 | 9.07e-16 | 72.0 |
| MS.gene91566.t1 | MTR_2g076060 | 51.471 | 68 | 22 | 2 | 3 | 59 | 18 | 85 | 8.90e-15 | 68.9 |
| MS.gene91566.t1 | MTR_4g021270 | 66.667 | 42 | 14 | 0 | 24 | 65 | 59 | 100 | 1.89e-14 | 69.3 |
| MS.gene91566.t1 | MTR_1g070250 | 61.364 | 44 | 17 | 0 | 21 | 64 | 35 | 78 | 1.02e-13 | 65.5 |
| MS.gene91566.t1 | MTR_5g014400 | 76.471 | 34 | 8 | 0 | 24 | 57 | 80 | 113 | 1.11e-13 | 66.2 |
| MS.gene91566.t1 | MTR_1g044225 | 65.000 | 40 | 14 | 0 | 24 | 63 | 26 | 65 | 1.20e-13 | 64.7 |
| MS.gene91566.t1 | MTR_1g070265 | 64.286 | 42 | 13 | 1 | 15 | 56 | 28 | 67 | 1.50e-13 | 65.1 |
| MS.gene91566.t1 | MTR_5g080660 | 74.359 | 39 | 10 | 0 | 24 | 62 | 47 | 85 | 1.76e-13 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91566.t1 | AT3G09290 | 37.278 | 169 | 62 | 6 | 1 | 131 | 1 | 163 | 3.85e-19 | 79.3 |
| MS.gene91566.t1 | AT3G53820 | 35.862 | 145 | 54 | 5 | 15 | 139 | 13 | 138 | 1.32e-16 | 72.0 |
| MS.gene91566.t1 | AT5G06070 | 80.556 | 36 | 7 | 0 | 24 | 59 | 53 | 88 | 6.44e-15 | 69.3 |
| MS.gene91566.t1 | AT2G42410 | 56.897 | 58 | 23 | 1 | 20 | 75 | 43 | 100 | 9.87e-15 | 68.6 |
| MS.gene91566.t1 | AT2G42410 | 56.897 | 58 | 23 | 1 | 20 | 75 | 43 | 100 | 9.87e-15 | 68.6 |
| MS.gene91566.t1 | AT2G42410 | 56.897 | 58 | 23 | 1 | 20 | 75 | 69 | 126 | 1.38e-14 | 68.6 |
| MS.gene91566.t1 | AT2G37740 | 47.826 | 69 | 35 | 1 | 24 | 92 | 37 | 104 | 1.55e-14 | 69.3 |
| MS.gene91566.t1 | AT5G43540 | 39.252 | 107 | 59 | 2 | 26 | 131 | 35 | 136 | 7.47e-14 | 64.7 |
| MS.gene91566.t1 | AT3G23130 | 74.286 | 35 | 9 | 0 | 24 | 58 | 45 | 79 | 1.65e-13 | 65.1 |
| MS.gene91566.t1 | AT4G17810 | 33.846 | 130 | 67 | 4 | 24 | 134 | 44 | 173 | 1.04e-11 | 59.7 |
| MS.gene91566.t1 | AT4G17810 | 76.667 | 30 | 7 | 0 | 24 | 53 | 44 | 73 | 2.43e-11 | 59.3 |
Find 33 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAAGAAAATTTGCTTTCTT+TGG | 0.082851 | 7.1:+38584800 | MS.gene91566:CDS |
| CTTATTTGGTCAGATTTATT+AGG | 0.139528 | 7.1:-38584639 | None:intergenic |
| GGTCAGATTTATTAGGGTTT+TGG | 0.161658 | 7.1:-38584632 | None:intergenic |
| CGTCAGAGCTCCATCTTATT+TGG | 0.226034 | 7.1:-38584653 | None:intergenic |
| TCCGAATCTGCTTGATCATT+AGG | 0.285392 | 7.1:-38584840 | None:intergenic |
| GCTTGGCTCTATCTCTTCTA+TGG | 0.291132 | 7.1:-38584761 | None:intergenic |
| CCGAATCTGCTTGATCATTA+GGG | 0.317565 | 7.1:-38584839 | None:intergenic |
| TTATTTGGTCAGATTTATTA+GGG | 0.342818 | 7.1:-38584638 | None:intergenic |
| GTTCTTCATTTGTTGACATA+AGG | 0.348978 | 7.1:+38584919 | MS.gene91566:CDS |
| TTCTTCATTTGTTGACATAA+GGG | 0.367087 | 7.1:+38584920 | MS.gene91566:CDS |
| TTCTCTAATGCACAAGCATT+AGG | 0.389376 | 7.1:+38584723 | MS.gene91566:CDS |
| CCCTAATGATCAAGCAGATT+CGG | 0.394788 | 7.1:+38584839 | MS.gene91566:CDS |
| AAGGGGATCCAGTGGTGTTA+TGG | 0.404055 | 7.1:+38584938 | MS.gene91566:CDS |
| CTGACGATCCTGGTCAAGTT+AGG | 0.408639 | 7.1:+38584670 | MS.gene91566:CDS |
| GGAAGATTGTTGTTTGAGCT+TGG | 0.430049 | 7.1:-38584778 | None:intergenic |
| TTAGACCTTGAGCTTAGGTT+AGG | 0.446249 | 7.1:+38584990 | MS.gene91566:CDS |
| GTGATTTAGACCTTGAGCTT+AGG | 0.467678 | 7.1:+38584985 | MS.gene91566:CDS |
| AGAGAATCATGAAACTCAAA+GGG | 0.481266 | 7.1:+38584863 | MS.gene91566:CDS |
| ATAAATCTGACCAAATAAGA+TGG | 0.504424 | 7.1:+38584643 | MS.gene91566:CDS |
| TAGGTTAGGTTATCAGCCAA+TGG | 0.520042 | 7.1:+38585004 | MS.gene91566:CDS |
| AGATGGAGCTCTGACGATCC+TGG | 0.523408 | 7.1:+38584660 | MS.gene91566:CDS |
| CTCTTCTATGGATATTCATG+TGG | 0.536618 | 7.1:-38584749 | None:intergenic |
| AAGAGAATCATGAAACTCAA+AGG | 0.543574 | 7.1:+38584862 | MS.gene91566:CDS |
| TCTTCATTTGTTGACATAAG+GGG | 0.545273 | 7.1:+38584921 | MS.gene91566:CDS |
| CCAGTGGTGTTATGGCTCAA+AGG | 0.569482 | 7.1:+38584946 | MS.gene91566:CDS |
| CCTTTGAGCCATAACACCAC+TGG | 0.579057 | 7.1:-38584946 | None:intergenic |
| CTCAATGTTGATGAATCCAT+TGG | 0.590079 | 7.1:-38585020 | None:intergenic |
| TCAACAAATGAAGAACTATG+AGG | 0.591937 | 7.1:-38584912 | None:intergenic |
| TCTAATGCACAAGCATTAGG+AGG | 0.598923 | 7.1:+38584726 | MS.gene91566:CDS |
| GAATAAGACCTAACTTGACC+AGG | 0.612183 | 7.1:-38584678 | None:intergenic |
| GATAACCTAACCTAAGCTCA+AGG | 0.631813 | 7.1:-38584995 | None:intergenic |
| GTTGACATAAGGGGATCCAG+TGG | 0.671698 | 7.1:+38584930 | MS.gene91566:CDS |
| GAGAATCATGAAACTCAAAG+GGG | 0.747840 | 7.1:+38584864 | MS.gene91566:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTTGGTCAGATTTATTA+GGG | - | chr7.1:38584641-38584660 | None:intergenic | 20.0% |
| ! | ATAAATCTGACCAAATAAGA+TGG | + | chr7.1:38584643-38584662 | MS.gene91566:CDS | 25.0% |
| ! | CTTATTTGGTCAGATTTATT+AGG | - | chr7.1:38584642-38584661 | None:intergenic | 25.0% |
| !! | CAAAGAAAGCAAATTTTCTT+CGG | - | chr7.1:38584802-38584821 | None:intergenic | 25.0% |
| !! | TTCTTCATTTGTTGACATAA+GGG | + | chr7.1:38584920-38584939 | MS.gene91566:CDS | 25.0% |
| AAGAGAATCATGAAACTCAA+AGG | + | chr7.1:38584862-38584881 | MS.gene91566:CDS | 30.0% | |
| AGAGAATCATGAAACTCAAA+GGG | + | chr7.1:38584863-38584882 | MS.gene91566:CDS | 30.0% | |
| TCAACAAATGAAGAACTATG+AGG | - | chr7.1:38584915-38584934 | None:intergenic | 30.0% | |
| ! | CGAAGAAAATTTGCTTTCTT+TGG | + | chr7.1:38584800-38584819 | MS.gene91566:CDS | 30.0% |
| ! | GTCAGATTTATTAGGGTTTT+GGG | - | chr7.1:38584634-38584653 | None:intergenic | 30.0% |
| ! | GTTCTTCATTTGTTGACATA+AGG | + | chr7.1:38584919-38584938 | MS.gene91566:CDS | 30.0% |
| ! | TCTTCATTTGTTGACATAAG+GGG | + | chr7.1:38584921-38584940 | MS.gene91566:CDS | 30.0% |
| CTCTTCTATGGATATTCATG+TGG | - | chr7.1:38584752-38584771 | None:intergenic | 35.0% | |
| GAGAATCATGAAACTCAAAG+GGG | + | chr7.1:38584864-38584883 | MS.gene91566:CDS | 35.0% | |
| TTCTCTAATGCACAAGCATT+AGG | + | chr7.1:38584723-38584742 | MS.gene91566:CDS | 35.0% | |
| ! | CTCAATGTTGATGAATCCAT+TGG | - | chr7.1:38585023-38585042 | None:intergenic | 35.0% |
| ! | GGTCAGATTTATTAGGGTTT+TGG | - | chr7.1:38584635-38584654 | None:intergenic | 35.0% |
| !! | TCTTGTACTTTTTGCAAGAG+AGG | + | chr7.1:38584699-38584718 | MS.gene91566:CDS | 35.0% |
| CCCTAATGATCAAGCAGATT+CGG | + | chr7.1:38584839-38584858 | MS.gene91566:CDS | 40.0% | |
| CCGAATCTGCTTGATCATTA+GGG | - | chr7.1:38584842-38584861 | None:intergenic | 40.0% | |
| GAATAAGACCTAACTTGACC+AGG | - | chr7.1:38584681-38584700 | None:intergenic | 40.0% | |
| GATAACCTAACCTAAGCTCA+AGG | - | chr7.1:38584998-38585017 | None:intergenic | 40.0% | |
| GTGATTTAGACCTTGAGCTT+AGG | + | chr7.1:38584985-38585004 | MS.gene91566:CDS | 40.0% | |
| TAGGTTAGGTTATCAGCCAA+TGG | + | chr7.1:38585004-38585023 | MS.gene91566:CDS | 40.0% | |
| TCCGAATCTGCTTGATCATT+AGG | - | chr7.1:38584843-38584862 | None:intergenic | 40.0% | |
| TCTAATGCACAAGCATTAGG+AGG | + | chr7.1:38584726-38584745 | MS.gene91566:CDS | 40.0% | |
| TTAGACCTTGAGCTTAGGTT+AGG | + | chr7.1:38584990-38585009 | MS.gene91566:CDS | 40.0% | |
| !! | GGAAGATTGTTGTTTGAGCT+TGG | - | chr7.1:38584781-38584800 | None:intergenic | 40.0% |
| CGTCAGAGCTCCATCTTATT+TGG | - | chr7.1:38584656-38584675 | None:intergenic | 45.0% | |
| !! | GCTTGGCTCTATCTCTTCTA+TGG | - | chr7.1:38584764-38584783 | None:intergenic | 45.0% |
| CCTTTGAGCCATAACACCAC+TGG | - | chr7.1:38584949-38584968 | None:intergenic | 50.0% | |
| CTGACGATCCTGGTCAAGTT+AGG | + | chr7.1:38584670-38584689 | MS.gene91566:CDS | 50.0% | |
| GTTGACATAAGGGGATCCAG+TGG | + | chr7.1:38584930-38584949 | MS.gene91566:CDS | 50.0% | |
| ! | AAGGGGATCCAGTGGTGTTA+TGG | + | chr7.1:38584938-38584957 | MS.gene91566:CDS | 50.0% |
| !! | CCAGTGGTGTTATGGCTCAA+AGG | + | chr7.1:38584946-38584965 | MS.gene91566:CDS | 50.0% |
| ! | AGATGGAGCTCTGACGATCC+TGG | + | chr7.1:38584660-38584679 | MS.gene91566:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 38584621 | 38585061 | 38584621 | ID=MS.gene91566 |
| chr7.1 | mRNA | 38584621 | 38585061 | 38584621 | ID=MS.gene91566.t1;Parent=MS.gene91566 |
| chr7.1 | exon | 38584621 | 38585061 | 38584621 | ID=MS.gene91566.t1.exon1;Parent=MS.gene91566.t1 |
| chr7.1 | CDS | 38584621 | 38585061 | 38584621 | ID=cds.MS.gene91566.t1;Parent=MS.gene91566.t1 |
| Gene Sequence |
| Protein sequence |
