Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91722.t1 | XP_013448846.1 | 96.2 | 78 | 3 | 0 | 1 | 78 | 1 | 78 | 1.20E-29 | 138.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91722.t1 | Q41657 | 87.2 | 78 | 9 | 1 | 1 | 78 | 1 | 77 | 1.1e-26 | 120.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91722.t1 | A0A072U177 | 96.2 | 78 | 3 | 0 | 1 | 78 | 1 | 78 | 8.6e-30 | 138.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051241 | MS.gene91722 | 0.803598 | 3.23E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91722.t1 | MTR_7g061018 | 94.872 | 78 | 4 | 0 | 1 | 78 | 1 | 78 | 5.02e-31 | 103 |
| MS.gene91722.t1 | MTR_4g021730 | 55.405 | 74 | 30 | 2 | 7 | 78 | 3 | 75 | 8.84e-18 | 70.5 |
| MS.gene91722.t1 | MTR_4g021725 | 53.247 | 77 | 27 | 3 | 4 | 78 | 6 | 75 | 1.76e-15 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91722.t1 | AT3G09390 | 70.370 | 81 | 21 | 3 | 1 | 78 | 1 | 81 | 3.02e-12 | 56.6 |
| MS.gene91722.t1 | AT3G09390 | 70.370 | 81 | 21 | 3 | 1 | 78 | 1 | 81 | 3.02e-12 | 56.6 |
| MS.gene91722.t1 | AT5G02380 | 70.000 | 80 | 19 | 3 | 1 | 78 | 1 | 77 | 2.86e-11 | 53.9 |
Find 26 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTGAGAGTGCTGCTGAAA+TGG | 0.348838 | 7.2:-38906128 | MS.gene91722:CDS |
| ACAGCCAGTGCCGCATTTGC+AGG | 0.352772 | 7.2:+38906913 | None:intergenic |
| TGCAAATGCGGCACTGGCTG+TGG | 0.361672 | 7.2:-38906911 | MS.gene91722:CDS |
| GATTCTGTGTAGCTCAAATC+AGG | 0.372420 | 7.2:+38906199 | None:intergenic |
| CAGGTTCATTTGCAAGTGCA+TGG | 0.385728 | 7.2:+38906058 | None:intergenic |
| AGTGCCTGCAAATGCGGCAC+TGG | 0.424055 | 7.2:-38906917 | MS.gene91722:CDS |
| CTGAAAATGTCTTGCTGTGG+TGG | 0.438298 | 7.2:-38906956 | None:intergenic |
| CATGGGTTGCAGCTGCAGTT+TGG | 0.448219 | 7.2:+38906076 | None:intergenic |
| TCAGCAGCACTCTCAAATTG+AGG | 0.485132 | 7.2:+38906133 | None:intergenic |
| AGGTTCATTTGCAAGTGCAT+GGG | 0.494035 | 7.2:+38906059 | None:intergenic |
| AAATGCGGCACTGGCTGTGG+AGG | 0.501749 | 7.2:-38906908 | MS.gene91722:CDS |
| TTTGAGAGTGCTGCTGAAAT+GGG | 0.507034 | 7.2:-38906127 | MS.gene91722:CDS |
| AGCACTCTCAAATTGAGGCT+TGG | 0.528291 | 7.2:+38906138 | None:intergenic |
| AACCACTGAGACCCTTGTGA+TGG | 0.567822 | 7.2:-38906173 | MS.gene91722:CDS |
| ACCACTGAGACCCTTGTGAT+GGG | 0.567834 | 7.2:-38906172 | MS.gene91722:CDS |
| GAGAATGATGGCTGCAAGTG+TGG | 0.583748 | 7.2:-38906100 | MS.gene91722:CDS |
| CAGATGCAACTCCCATCACA+AGG | 0.587599 | 7.2:+38906161 | None:intergenic |
| TCTTGCTGTGGTGGAAACTG+TGG | 0.591533 | 7.2:-38906947 | MS.gene91722:CDS |
| TGTGGTGGAAACTGTGGTTG+TGG | 0.595875 | 7.2:-38906941 | MS.gene91722:CDS |
| TGTGGAAGTGCCTGCAAATG+CGG | 0.600913 | 7.2:-38906923 | MS.gene91722:CDS |
| GAAATGGGTGCTGAGAATGA+TGG | 0.610124 | 7.2:-38906112 | MS.gene91722:CDS |
| TCCCATCACAAGGGTCTCAG+TGG | 0.626453 | 7.2:+38906171 | None:intergenic |
| AATGCGGCACTGGCTGTGGA+GGG | 0.639645 | 7.2:-38906907 | MS.gene91722:intron |
| ATTCTGTGTAGCTCAAATCA+GGG | 0.645627 | 7.2:+38906200 | None:intergenic |
| CATCTGAAAATGTCTTGCTG+TGG | 0.660819 | 7.2:-38906959 | None:intergenic |
| AGATGCAACTCCCATCACAA+GGG | 0.706946 | 7.2:+38906162 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATTTAACAAAATATTTCT+AGG | - | chr7.2:38906704-38906723 | MS.gene91722:intron | 10.0% |
| !!! | TAATTCATTGATTATTTTAT+GGG | - | chr7.2:38906436-38906455 | MS.gene91722:intron | 10.0% |
| !!! | TTAATTCATTGATTATTTTA+TGG | - | chr7.2:38906435-38906454 | MS.gene91722:intron | 10.0% |
| !! | TAAAATTTAACTAAACCAAA+GGG | + | chr7.2:38906466-38906485 | None:intergenic | 15.0% |
| !! | TTAAAATTTAACTAAACCAA+AGG | + | chr7.2:38906467-38906486 | None:intergenic | 15.0% |
| !!! | ATTCATGTCTTTTTTTTTTT+TGG | - | chr7.2:38906139-38906158 | MS.gene91722:CDS | 15.0% |
| !!! | TTCTTTTGATTTGATTTTTT+TGG | - | chr7.2:38906220-38906239 | MS.gene91722:intron | 15.0% |
| !! | AAAATTTAACTAAACCAAAG+GGG | + | chr7.2:38906465-38906484 | None:intergenic | 20.0% |
| !! | ATTTCTTCAAATTCAATTAG+AGG | + | chr7.2:38906406-38906425 | None:intergenic | 20.0% |
| !!! | GAGCTTTTTGTATTTTTTTT+GGG | - | chr7.2:38906340-38906359 | MS.gene91722:intron | 20.0% |
| !!! | TGAGCTTTTTGTATTTTTTT+TGG | - | chr7.2:38906339-38906358 | MS.gene91722:intron | 20.0% |
| !!! | TGTATTTTTCATTTGCTTTA+TGG | - | chr7.2:38906581-38906600 | MS.gene91722:intron | 20.0% |
| ! | TGAAATTGAACAAAAGCTAA+TGG | + | chr7.2:38906545-38906564 | None:intergenic | 25.0% |
| ! | TTGGGTAAATTGTTTCATAT+GGG | - | chr7.2:38906358-38906377 | MS.gene91722:intron | 25.0% |
| ! | TTTGGGTAAATTGTTTCATA+TGG | - | chr7.2:38906357-38906376 | MS.gene91722:intron | 25.0% |
| ! | TTTGTTCAATTTCACAAAGA+TGG | - | chr7.2:38906551-38906570 | MS.gene91722:intron | 25.0% |
| !! | AGTCAATTGTTGATTAAGAA+GGG | + | chr7.2:38906616-38906635 | None:intergenic | 25.0% |
| !! | TAGTCAATTGTTGATTAAGA+AGG | + | chr7.2:38906617-38906636 | None:intergenic | 25.0% |
| AAGAAACAGAAAACTTTCTC+TGG | + | chr7.2:38906205-38906224 | None:intergenic | 30.0% | |
| TCCATGCAATTCATCAAAAA+AGG | + | chr7.2:38906248-38906267 | None:intergenic | 30.0% | |
| TGGGTAAATTGTTTCATATG+GGG | - | chr7.2:38906359-38906378 | MS.gene91722:intron | 30.0% | |
| TTTCATTTGCTTTATGGCTT+TGG | - | chr7.2:38906587-38906606 | MS.gene91722:intron | 30.0% | |
| ! | GCTACGTTTCAATTTTGTAT+TGG | - | chr7.2:38906653-38906672 | MS.gene91722:intron | 30.0% |
| !!! | ACCTTTTTTGATGAATTGCA+TGG | - | chr7.2:38906244-38906263 | MS.gene91722:intron | 30.0% |
| ATTCTGTGTAGCTCAAATCA+GGG | + | chr7.2:38906816-38906835 | None:intergenic | 35.0% | |
| TTAACCCTTCCTCTTATGTT+GGG | - | chr7.2:38906282-38906301 | MS.gene91722:intron | 35.0% | |
| TTTAACCCTTCCTCTTATGT+TGG | - | chr7.2:38906281-38906300 | MS.gene91722:intron | 35.0% | |
| ! | ATTTTCCCAACATAAGAGGA+AGG | + | chr7.2:38906290-38906309 | None:intergenic | 35.0% |
| ! | CACAATTTTCCCAACATAAG+AGG | + | chr7.2:38906294-38906313 | None:intergenic | 35.0% |
| ! | TATTTTATGGGTATCCCCTT+TGG | - | chr7.2:38906448-38906467 | MS.gene91722:intron | 35.0% |
| ! | TTTTCCCAACATAAGAGGAA+GGG | + | chr7.2:38906289-38906308 | None:intergenic | 35.0% |
| GATTCTGTGTAGCTCAAATC+AGG | + | chr7.2:38906817-38906836 | None:intergenic | 40.0% | |
| !! | ATTTGAGAGTGCTGCTGAAA+TGG | - | chr7.2:38906885-38906904 | MS.gene91722:intron | 40.0% |
| !! | TTTGAGAGTGCTGCTGAAAT+GGG | - | chr7.2:38906886-38906905 | MS.gene91722:intron | 40.0% |
| AGATGCAACTCCCATCACAA+GGG | + | chr7.2:38906854-38906873 | None:intergenic | 45.0% | |
| AGCACTCTCAAATTGAGGCT+TGG | + | chr7.2:38906878-38906897 | None:intergenic | 45.0% | |
| TCAGCAGCACTCTCAAATTG+AGG | + | chr7.2:38906883-38906902 | None:intergenic | 45.0% | |
| !! | GAAATGGGTGCTGAGAATGA+TGG | - | chr7.2:38906901-38906920 | MS.gene91722:intron | 45.0% |
| !!! | TAGAAATTTTTAAATAAAAA+AGG | + | chr7.2:38906679-38906698 | None:intergenic | 5.0% |
| AACCACTGAGACCCTTGTGA+TGG | - | chr7.2:38906840-38906859 | MS.gene91722:intron | 50.0% | |
| ACCACTGAGACCCTTGTGAT+GGG | - | chr7.2:38906841-38906860 | MS.gene91722:intron | 50.0% | |
| CAGATGCAACTCCCATCACA+AGG | + | chr7.2:38906855-38906874 | None:intergenic | 50.0% | |
| GAGAATGATGGCTGCAAGTG+TGG | - | chr7.2:38906913-38906932 | MS.gene91722:CDS | 50.0% | |
| ! | TCTTGCTGTGGTGGAAACTG+TGG | - | chr7.2:38906066-38906085 | MS.gene91722:CDS | 50.0% |
| ! | TGTGGTGGAAACTGTGGTTG+TGG | - | chr7.2:38906072-38906091 | MS.gene91722:CDS | 50.0% |
| !! | TGTGGAAGTGCCTGCAAATG+CGG | - | chr7.2:38906090-38906109 | MS.gene91722:CDS | 50.0% |
| TCCCATCACAAGGGTCTCAG+TGG | + | chr7.2:38906845-38906864 | None:intergenic | 55.0% | |
| ! | CATGGGTTGCAGCTGCAGTT+TGG | + | chr7.2:38906940-38906959 | None:intergenic | 55.0% |
| ACAGCCAGTGCCGCATTTGC+AGG | + | chr7.2:38906103-38906122 | None:intergenic | 60.0% | |
| AGTGCCTGCAAATGCGGCAC+TGG | - | chr7.2:38906096-38906115 | MS.gene91722:CDS | 60.0% | |
| !! | AAATGCGGCACTGGCTGTGG+AGG | - | chr7.2:38906105-38906124 | MS.gene91722:CDS | 60.0% |
| !! | AATGCGGCACTGGCTGTGGA+GGG | - | chr7.2:38906106-38906125 | MS.gene91722:CDS | 60.0% |
| !! | TGCAAATGCGGCACTGGCTG+TGG | - | chr7.2:38906102-38906121 | MS.gene91722:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 38906063 | 38906972 | 38906063 | ID=MS.gene91722 |
| chr7.2 | mRNA | 38906063 | 38906972 | 38906063 | ID=MS.gene91722.t1;Parent=MS.gene91722 |
| chr7.2 | exon | 38906908 | 38906972 | 38906908 | ID=MS.gene91722.t1.exon1;Parent=MS.gene91722.t1 |
| chr7.2 | CDS | 38906908 | 38906972 | 38906908 | ID=cds.MS.gene91722.t1;Parent=MS.gene91722.t1 |
| chr7.2 | exon | 38906063 | 38906234 | 38906063 | ID=MS.gene91722.t1.exon2;Parent=MS.gene91722.t1 |
| chr7.2 | CDS | 38906063 | 38906234 | 38906063 | ID=cds.MS.gene91722.t1;Parent=MS.gene91722.t1 |
| Gene Sequence |
| Protein sequence |
