AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91902

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91902.t1 MTR_5g039600 95.215 418 15 3 1 415 1 416 0.0 669
MS.gene91902.t1 MTR_4g108370 48.064 439 149 19 16 412 27 428 1.96e-89 278
MS.gene91902.t1 MTR_2g006150 67.188 128 35 2 21 148 91 211 9.29e-50 174
MS.gene91902.t1 MTR_2g006150 67.188 128 35 2 21 148 91 211 1.13e-49 175
MS.gene91902.t1 MTR_7g028160 59.677 124 33 3 55 178 38 144 2.38e-39 142
MS.gene91902.t1 MTR_1g114380 71.277 94 18 2 50 139 17 105 2.92e-38 139
MS.gene91902.t1 MTR_8g033070 79.221 77 16 0 63 139 76 152 7.98e-37 137
MS.gene91902.t1 MTR_1g038650 78.205 78 12 1 58 135 28 100 5.05e-36 132
MS.gene91902.t1 MTR_1g063870 79.412 68 14 0 62 129 44 111 1.80e-33 124
MS.gene91902.t1 MTR_1g101810 67.059 85 28 0 64 148 61 145 5.92e-33 127
MS.gene91902.t1 MTR_1g101810 67.059 85 28 0 64 148 91 175 1.23e-32 126
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91902.t1 AT3G47620 46.108 334 113 13 58 352 108 413 1.97e-51 180
MS.gene91902.t1 AT1G69690 71.429 126 17 2 16 136 10 121 2.13e-51 176
MS.gene91902.t1 AT1G58100 88.235 85 10 0 64 148 57 141 1.56e-46 165
MS.gene91902.t1 AT1G35560 88.372 86 9 1 63 148 52 136 2.09e-44 157
MS.gene91902.t1 AT1G72010 87.342 79 10 0 64 142 60 138 8.12e-42 152
MS.gene91902.t1 AT2G45680 76.471 85 20 0 60 144 68 152 1.53e-38 142
MS.gene91902.t1 AT5G51910 74.699 83 21 0 57 139 47 129 1.54e-37 138
MS.gene91902.t1 AT5G51910 74.699 83 21 0 57 139 47 129 1.54e-37 138
MS.gene91902.t1 AT5G23280 74.390 82 16 1 58 139 35 111 1.06e-36 134
MS.gene91902.t1 AT3G27010 54.688 128 42 2 17 128 12 139 9.76e-36 134
MS.gene91902.t1 AT3G27010 54.688 128 42 2 17 128 12 139 9.76e-36 134
MS.gene91902.t1 AT5G08330 73.494 83 17 1 58 140 27 104 2.64e-35 130
MS.gene91902.t1 AT2G37000 77.419 62 14 0 65 126 41 102 1.13e-27 108
MS.gene91902.t1 AT5G41030 58.228 79 27 1 51 129 58 130 1.61e-26 107
MS.gene91902.t1 AT1G58100 62.353 85 8 1 64 148 57 117 7.47e-24 102
MS.gene91902.t1 AT3G45150 60.317 63 25 0 64 126 14 76 2.22e-21 90.9

Find 130 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAATTATTATCACAAAATA+TGG 0.184669 5.3:-27749926 MS.gene91902:CDS
TAAAAGCTGAAGAGGGAAAT+TGG 0.195439 5.3:+27750659 None:intergenic
GGCAGTACAACCATCGTTTC+CGG 0.257678 5.3:+27750581 None:intergenic
CCTGAACCTAGTTGACTTCC+AGG 0.283489 5.3:+27749664 None:intergenic
GTAATGGAAATGATCCAATT+TGG 0.290902 5.3:-27749789 MS.gene91902:CDS
CTGCTGGTCTGATGAGATTA+TGG 0.298113 5.3:+27750560 None:intergenic
ACAGAAGGAATTGCCCAAAT+TGG 0.300472 5.3:+27749775 None:intergenic
AAGCCGCCGGCATGCGGATC+CGG 0.301056 5.3:+27750472 None:intergenic
CTTCAAATACGGCAGCGTTT+TGG 0.303402 5.3:-27749855 MS.gene91902:CDS
CTTCCAGGCATAAGATTCAT+TGG 0.326078 5.3:+27749679 None:intergenic
GGTGCTCATCATCAAAATAA+TGG 0.332278 5.3:-27750696 MS.gene91902:CDS
GTTCCAGCTCACGCGTGAGC+TGG 0.358435 5.3:-27750436 MS.gene91902:CDS
GGCCGGAATTGTTCCTGTTC+CGG 0.366988 5.3:+27750344 None:intergenic
TCAGATTCAGATGGAAGTAT+AGG 0.367930 5.3:-27749970 MS.gene91902:CDS
AAGCATACTCAGATTGCTTT+CGG 0.377552 5.3:+27749603 None:intergenic
GTCATCGCCGCTACCGGAAC+AGG 0.388365 5.3:-27750357 MS.gene91902:CDS
CAGATTCAGATGGAAGTATA+GGG 0.391222 5.3:-27749969 MS.gene91902:CDS
GGCGGTGGAGGAGGAGGAGG+TGG 0.393868 5.3:-27749637 MS.gene91902:CDS
GGTGGAGGAGGAGGAGGTGG+TGG 0.394715 5.3:-27749634 MS.gene91902:CDS
GATTGAGTGGCTGCTTCAAC+AGG 0.397983 5.3:-27750391 MS.gene91902:CDS
CAGCATCACGGCGGAGGTGG+AGG 0.398279 5.3:-27749523 MS.gene91902:CDS
ATCGGCGGTGGAGGAGGAGG+AGG 0.403325 5.3:-27749640 MS.gene91902:CDS
TACAAGCAAAACAAGAGCTT+CGG 0.403614 5.3:-27750047 MS.gene91902:CDS
TAATGGAAATGATCCAATTT+GGG 0.409142 5.3:-27749788 MS.gene91902:CDS
TCCTTCGTCGAAGCGCGCTT+CGG 0.409305 5.3:+27750525 None:intergenic
AGCTTAGAAACCGGGCTGAA+TGG 0.413702 5.3:-27750206 MS.gene91902:CDS
GTCGAAGCGCGCTTCGGCGG+AGG 0.423149 5.3:+27750531 None:intergenic
TTTGGATGGTAACTGGTAAT+GGG 0.423705 5.3:-27749837 MS.gene91902:CDS
ACTGCCACCGCCTCCGGTGG+TGG 0.424243 5.3:-27750015 MS.gene91902:CDS
CGTCGGCTCAGCTTAGAAAC+CGG 0.427388 5.3:-27750215 MS.gene91902:CDS
GACCGGCACACGAAGGTAGA+TGG 0.427587 5.3:-27750504 MS.gene91902:CDS
TCAGGAATCGGCGGTGGAGG+AGG 0.431745 5.3:-27749646 MS.gene91902:CDS
TTCAAGTCATGCTTCAAATA+CGG 0.432008 5.3:-27749866 MS.gene91902:CDS
GGAATCGGCGGTGGAGGAGG+AGG 0.432136 5.3:-27749643 MS.gene91902:CDS
TACCGGAACAGGAACAATTC+CGG 0.434355 5.3:-27750346 MS.gene91902:CDS
AACATTGCTGATAAAGAAGA+AGG 0.434505 5.3:+27750099 None:intergenic
GGAAACAATGGTTTATATAG+AGG 0.437006 5.3:-27749751 MS.gene91902:CDS
AAAAGCTGAAGAGGGAAATT+GGG 0.437875 5.3:+27750660 None:intergenic
AAGCAATCTGAGTATGCTTT+CGG 0.438504 5.3:-27749599 MS.gene91902:CDS
GAGGGAAATTGGGTCTGCTT+CGG 0.440519 5.3:+27750670 None:intergenic
GCTTAGAAACCGGGCTGAAT+GGG 0.442453 5.3:-27750205 MS.gene91902:CDS
ACCGCCTCCGGTGGTGGCGG+AGG 0.443245 5.3:-27750009 MS.gene91902:CDS
ATTTGGGCAATTCCTTCTGT+TGG 0.444072 5.3:-27749772 MS.gene91902:CDS
GGAGCTATGTCATCTCCTAA+TGG 0.444498 5.3:-27749730 MS.gene91902:CDS
GGCGGAGGATTTGAATTGTT+AGG 0.445132 5.3:-27749994 MS.gene91902:CDS
GGACAGCATCACGGCGGAGG+TGG 0.445597 5.3:-27749526 MS.gene91902:CDS
ATTCGTCGAAATCTGCCTAT+AGG 0.448820 5.3:+27749567 None:intergenic
GTCCCAGCTCACGCGTGAGC+TGG 0.449479 5.3:+27750433 None:intergenic
CCGGTAGCGGCGATGACAGC+TGG 0.456062 5.3:+27750363 None:intergenic
GCCGAAGCGCGCTTCGACGA+AGG 0.457337 5.3:-27750526 MS.gene91902:CDS
AATATATCTCTTAGAAGCTC+AGG 0.459346 5.3:-27750306 MS.gene91902:CDS
CCTGGAAGTCAACTAGGTTC+AGG 0.459463 5.3:-27749664 MS.gene91902:CDS
TTTGTTCTTCTGAGACATCT+AGG 0.461674 5.3:+27750616 None:intergenic
TCCTCCGCCACCACCGGAGG+CGG 0.462046 5.3:+27750008 None:intergenic
TATTTGTTACAATCAAATGT+AGG 0.463489 5.3:-27749898 MS.gene91902:CDS
ACAAGCAAAACAAGAGCTTC+GGG 0.465254 5.3:-27750046 MS.gene91902:CDS
TCCGCCACCACCGGAGGCGG+TGG 0.465294 5.3:+27750011 None:intergenic
CATCACGGCGGAGGTGGAGG+AGG 0.465897 5.3:-27749520 MS.gene91902:CDS
TTCCAGCTCACGCGTGAGCT+GGG 0.468128 5.3:-27750435 MS.gene91902:CDS
GAACACACTGCCACCGCCTC+CGG 0.472267 5.3:-27750021 MS.gene91902:CDS
GTCCATCTACCTTCGTGTGC+CGG 0.473163 5.3:+27750502 None:intergenic
GGTTCAGGAATCGGCGGTGG+AGG 0.473410 5.3:-27749649 MS.gene91902:CDS
CTTATGCCTGGAAGTCAACT+AGG 0.475042 5.3:-27749670 MS.gene91902:CDS
AATTGTTCCTGTTCCGGTAG+CGG 0.476759 5.3:+27750350 None:intergenic
GTATTTGAAGCATGACTTGA+AGG 0.481871 5.3:+27749868 None:intergenic
AGGTAGATGGACGAGGCCGC+CGG 0.488344 5.3:-27750491 MS.gene91902:CDS
CGGCACAAGCCGCCGGCATG+CGG 0.489708 5.3:+27750466 None:intergenic
TTCATGAAATGAAATCCATT+AGG 0.492064 5.3:+27749715 None:intergenic
GCCACCGCCTCCGGTGGTGG+CGG 0.492912 5.3:-27750012 MS.gene91902:CDS
GAAGTATAGGGAGGAAAAGA+AGG 0.493058 5.3:-27749957 MS.gene91902:CDS
GGAGGTGGAGGAGGAGATGA+TGG 0.493240 5.3:-27749511 MS.gene91902:CDS
CACCATTCACATTAACACCT+TGG 0.500135 5.3:+27749812 None:intergenic
CCAGCTGTCATCGCCGCTAC+CGG 0.502854 5.3:-27750363 MS.gene91902:CDS
TTGTAGCCGATGATTCTAGA+AGG 0.503590 5.3:-27750149 MS.gene91902:CDS
CCGCCGGCATGCGGATCCGG+CGG 0.503622 5.3:+27750475 None:intergenic
TACCTCGAAAATAGTGAGAC+GGG 0.503776 5.3:+27750268 None:intergenic
ATTTGTTACAATCAAATGTA+GGG 0.504618 5.3:-27749897 MS.gene91902:CDS
GACGCGGAGGAGAACGAACT+AGG 0.505339 5.3:+27750234 None:intergenic
TATATTAAGTGAAGTAAAGT+TGG 0.506746 5.3:+27750323 None:intergenic
AGCGCGCTTCGACGAAGGAC+CGG 0.508882 5.3:-27750521 MS.gene91902:CDS
GTTAATGTGAATGGTGGTAA+TGG 0.513343 5.3:-27749805 MS.gene91902:CDS
AGGCCGCCGGATCCGCATGC+CGG 0.515715 5.3:-27750478 MS.gene91902:CDS
TCGGGACAGCATCACGGCGG+AGG 0.519932 5.3:-27749529 MS.gene91902:CDS
TTTCGGCTATGAATGCCTAT+AGG 0.521533 5.3:-27749582 MS.gene91902:CDS
ATTCCTTCTGTTGGAAACAA+TGG 0.523024 5.3:-27749763 MS.gene91902:CDS
CCGCCGGATCCGCATGCCGG+CGG 0.526015 5.3:-27750475 MS.gene91902:CDS
AGTCAACTAGGTTCAGGAAT+CGG 0.526365 5.3:-27749658 MS.gene91902:CDS
AAACCATTGTTTCCAACAGA+AGG 0.531131 5.3:+27749760 None:intergenic
ACTCTGGCGGCACAAGCCGC+CGG 0.531379 5.3:+27750459 None:intergenic
TTCGTCGAAGCGCGCTTCGG+CGG 0.531746 5.3:+27750528 None:intergenic
GAATGGTATGTCAGATTCGT+CGG 0.533559 5.3:-27749548 MS.gene91902:CDS
TTCAAATCCTCCGCCACCAC+CGG 0.535080 5.3:+27750002 None:intergenic
TCTCCAATGAATCTTATGCC+TGG 0.535265 5.3:-27749682 MS.gene91902:CDS
GAATTATTATCACAAAATAT+GGG 0.535940 5.3:-27749925 MS.gene91902:CDS
CTAGGTTCAGGAATCGGCGG+TGG 0.540463 5.3:-27749652 MS.gene91902:CDS
AGCACGATCATGGAATTTGA+AGG 0.546398 5.3:-27750717 None:intergenic
AATGGTATGTCAGATTCGTC+GGG 0.560895 5.3:-27749547 MS.gene91902:CDS
TTAAGTGAAGTAAAGTTGGC+CGG 0.565359 5.3:+27750327 None:intergenic
AACCAAGGTGTTAATGTGAA+TGG 0.576736 5.3:-27749814 MS.gene91902:CDS
TCAAGCAGTCAGCATTCCTA+AGG 0.577498 5.3:-27749481 None:intergenic
ATAGTGAGACGGGGCAGACA+TGG 0.577717 5.3:+27750278 None:intergenic
ACTACTCCTTCTAGAATCAT+CGG 0.577923 5.3:+27750143 None:intergenic
TTGTTAGGTTCAGATTCAGA+TGG 0.580560 5.3:-27749979 MS.gene91902:CDS
ACGCGTGAGCTGGAACACTC+TGG 0.580832 5.3:+27750443 None:intergenic
TAGTTCTGTCCCATTCAGCC+CGG 0.583900 5.3:+27750196 None:intergenic
CACACTGCCACCGCCTCCGG+TGG 0.585290 5.3:-27750018 MS.gene91902:CDS
GAACAAACTTGTACAACCAC+CGG 0.588907 5.3:-27750600 MS.gene91902:CDS
AAATCCTCCGCCACCACCGG+AGG 0.591864 5.3:+27750005 None:intergenic
GTCGGCTCAGCTTAGAAACC+GGG 0.598655 5.3:-27750214 MS.gene91902:CDS
TATAGGCAGATTTCGACGAA+TGG 0.600746 5.3:-27749565 MS.gene91902:CDS
GAGTAGTAACTCTACCATGT+TGG 0.612610 5.3:-27750127 MS.gene91902:CDS
GAGCTGGGACATAAATCAGA+TGG 0.615252 5.3:-27750420 MS.gene91902:CDS
TGTACAACCACCGGAAACGA+TGG 0.616353 5.3:-27750591 MS.gene91902:CDS
CACACGAAGGTAGATGGACG+AGG 0.621520 5.3:-27750498 MS.gene91902:CDS
ACGCGGAGGAGAACGAACTA+GGG 0.632379 5.3:+27750235 None:intergenic
TTACCTCGAAAATAGTGAGA+CGG 0.633250 5.3:+27750267 None:intergenic
GATTCGTCGGGACAGCATCA+CGG 0.641317 5.3:-27749535 MS.gene91902:CDS
GTTTCTAAGCTGAGCCGACG+CGG 0.645764 5.3:+27750218 None:intergenic
TAGTTCGTTCTCCTCCGCGT+CGG 0.648405 5.3:-27750232 MS.gene91902:CDS
AGTACAACCATCGTTTCCGG+TGG 0.648819 5.3:+27750584 None:intergenic
CAAGGTGTTAATGTGAATGG+TGG 0.667294 5.3:-27749811 MS.gene91902:CDS
GTAACTGGTAATGGGAACCA+AGG 0.670023 5.3:-27749829 MS.gene91902:CDS
CAACTAGGTTCAGGAATCGG+CGG 0.678265 5.3:-27749655 MS.gene91902:CDS
TCTAAGCTGAGCCGACGCGG+AGG 0.679234 5.3:+27750221 None:intergenic
ATTCAGATGGAAGTATAGGG+AGG 0.684188 5.3:-27749966 MS.gene91902:CDS
CAGATGGTGAGACGATTGAG+TGG 0.696529 5.3:-27750404 MS.gene91902:CDS
GACGAAGGACCGGCACACGA+AGG 0.699063 5.3:-27750511 MS.gene91902:CDS
ACCTCGAAAATAGTGAGACG+GGG 0.702901 5.3:+27750269 None:intergenic
CGTGAGCTGGAACACTCTGG+CGG 0.708891 5.3:+27750446 None:intergenic
TCGTCGGGACAGCATCACGG+CGG 0.801947 5.3:-27749532 MS.gene91902:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAATTATTATCACAAAATA+TGG - chr5.3:27750265-27750284 MS.gene91902:CDS 15.0%
!! GAATTATTATCACAAAATAT+GGG - chr5.3:27750266-27750285 MS.gene91902:CDS 15.0%
!! TATATTAAGTGAAGTAAAGT+TGG + chr5.3:27749871-27749890 None:intergenic 20.0%
!!! ATTTGTTACAATCAAATGTA+GGG - chr5.3:27750294-27750313 MS.gene91902:CDS 20.0%
!!! TATTTGTTACAATCAAATGT+AGG - chr5.3:27750293-27750312 MS.gene91902:CDS 20.0%
! TAATGGAAATGATCCAATTT+GGG - chr5.3:27750403-27750422 MS.gene91902:CDS 25.0%
! TTCATGAAATGAAATCCATT+AGG + chr5.3:27750479-27750498 None:intergenic 25.0%
AACATTGCTGATAAAGAAGA+AGG + chr5.3:27750095-27750114 None:intergenic 30.0%
AATATATCTCTTAGAAGCTC+AGG - chr5.3:27749885-27749904 MS.gene91902:CDS 30.0%
GGAAACAATGGTTTATATAG+AGG - chr5.3:27750440-27750459 MS.gene91902:CDS 30.0%
TTCAAGTCATGCTTCAAATA+CGG - chr5.3:27750325-27750344 MS.gene91902:CDS 30.0%
! GTAATGGAAATGATCCAATT+TGG - chr5.3:27750402-27750421 MS.gene91902:CDS 30.0%
!! TTTTCTCTAAAAGCTGAAGA+GGG + chr5.3:27749542-27749561 None:intergenic 30.0%
!! TTTTTCTCTAAAAGCTGAAG+AGG + chr5.3:27749543-27749562 None:intergenic 30.0%
AAAAGCTGAAGAGGGAAATT+GGG + chr5.3:27749534-27749553 None:intergenic 35.0%
AAACCATTGTTTCCAACAGA+AGG + chr5.3:27750434-27750453 None:intergenic 35.0%
AAGCAATCTGAGTATGCTTT+CGG - chr5.3:27750592-27750611 MS.gene91902:CDS 35.0%
AAGCATACTCAGATTGCTTT+CGG + chr5.3:27750591-27750610 None:intergenic 35.0%
ATTCCTTCTGTTGGAAACAA+TGG - chr5.3:27750428-27750447 MS.gene91902:CDS 35.0%
GGTGCTCATCATCAAAATAA+TGG - chr5.3:27749495-27749514 MS.gene91902:CDS 35.0%
GTTAATGTGAATGGTGGTAA+TGG - chr5.3:27750386-27750405 MS.gene91902:CDS 35.0%
TAAAAGCTGAAGAGGGAAAT+TGG + chr5.3:27749535-27749554 None:intergenic 35.0%
TACAAGCAAAACAAGAGCTT+CGG - chr5.3:27750144-27750163 MS.gene91902:CDS 35.0%
TTAAGTGAAGTAAAGTTGGC+CGG + chr5.3:27749867-27749886 None:intergenic 35.0%
TTACCTCGAAAATAGTGAGA+CGG + chr5.3:27749927-27749946 None:intergenic 35.0%
TTGTTAGGTTCAGATTCAGA+TGG - chr5.3:27750212-27750231 MS.gene91902:CDS 35.0%
TTTGGATGGTAACTGGTAAT+GGG - chr5.3:27750354-27750373 MS.gene91902:CDS 35.0%
! AACCAAGGTGTTAATGTGAA+TGG - chr5.3:27750377-27750396 MS.gene91902:CDS 35.0%
! AGAAGAAGGATTTTCCAACA+TGG + chr5.3:27750081-27750100 None:intergenic 35.0%
! CAGATTCAGATGGAAGTATA+GGG - chr5.3:27750222-27750241 MS.gene91902:CDS 35.0%
! GTATTTGAAGCATGACTTGA+AGG + chr5.3:27750326-27750345 None:intergenic 35.0%
! TCAGATTCAGATGGAAGTAT+AGG - chr5.3:27750221-27750240 MS.gene91902:CDS 35.0%
! TTTTGGATGGTAACTGGTAA+TGG - chr5.3:27750353-27750372 MS.gene91902:CDS 35.0%
!! ACTACTCCTTCTAGAATCAT+CGG + chr5.3:27750051-27750070 None:intergenic 35.0%
!! TTTGTTCTTCTGAGACATCT+AGG + chr5.3:27749578-27749597 None:intergenic 35.0%
AATGGTATGTCAGATTCGTC+GGG - chr5.3:27750644-27750663 MS.gene91902:CDS 40.0%
ACAAGCAAAACAAGAGCTTC+GGG - chr5.3:27750145-27750164 MS.gene91902:CDS 40.0%
ACAGAAGGAATTGCCCAAAT+TGG + chr5.3:27750419-27750438 None:intergenic 40.0%
AGTCAACTAGGTTCAGGAAT+CGG - chr5.3:27750533-27750552 MS.gene91902:CDS 40.0%
ATTCGTCGAAATCTGCCTAT+AGG + chr5.3:27750627-27750646 None:intergenic 40.0%
ATTTGGGCAATTCCTTCTGT+TGG - chr5.3:27750419-27750438 MS.gene91902:CDS 40.0%
CACCATTCACATTAACACCT+TGG + chr5.3:27750382-27750401 None:intergenic 40.0%
CTTCCAGGCATAAGATTCAT+TGG + chr5.3:27750515-27750534 None:intergenic 40.0%
GAACAAACTTGTACAACCAC+CGG - chr5.3:27749591-27749610 MS.gene91902:CDS 40.0%
GAAGTATAGGGAGGAAAAGA+AGG - chr5.3:27750234-27750253 MS.gene91902:CDS 40.0%
GAATGGTATGTCAGATTCGT+CGG - chr5.3:27750643-27750662 MS.gene91902:CDS 40.0%
GAGTAGTAACTCTACCATGT+TGG - chr5.3:27750064-27750083 MS.gene91902:CDS 40.0%
TACCTCGAAAATAGTGAGAC+GGG + chr5.3:27749926-27749945 None:intergenic 40.0%
TATAGGCAGATTTCGACGAA+TGG - chr5.3:27750626-27750645 MS.gene91902:CDS 40.0%
TCTCCAATGAATCTTATGCC+TGG - chr5.3:27750509-27750528 MS.gene91902:CDS 40.0%
TTGTAGCCGATGATTCTAGA+AGG - chr5.3:27750042-27750061 MS.gene91902:CDS 40.0%
TTTCGGCTATGAATGCCTAT+AGG - chr5.3:27750609-27750628 MS.gene91902:CDS 40.0%
! ATTCAGATGGAAGTATAGGG+AGG - chr5.3:27750225-27750244 MS.gene91902:CDS 40.0%
! CAAGGTGTTAATGTGAATGG+TGG - chr5.3:27750380-27750399 MS.gene91902:CDS 40.0%
AATTGTTCCTGTTCCGGTAG+CGG + chr5.3:27749844-27749863 None:intergenic 45.0%
ACCTCGAAAATAGTGAGACG+GGG + chr5.3:27749925-27749944 None:intergenic 45.0%
CTTATGCCTGGAAGTCAACT+AGG - chr5.3:27750521-27750540 MS.gene91902:CDS 45.0%
GAGCTGGGACATAAATCAGA+TGG - chr5.3:27749771-27749790 MS.gene91902:CDS 45.0%
GGAGCTATGTCATCTCCTAA+TGG - chr5.3:27750461-27750480 MS.gene91902:CDS 45.0%
GGCGGAGGATTTGAATTGTT+AGG - chr5.3:27750197-27750216 MS.gene91902:CDS 45.0%
TACCGGAACAGGAACAATTC+CGG - chr5.3:27749845-27749864 MS.gene91902:CDS 45.0%
! CTGCTGGTCTGATGAGATTA+TGG + chr5.3:27749634-27749653 None:intergenic 45.0%
! CTTCAAATACGGCAGCGTTT+TGG - chr5.3:27750336-27750355 MS.gene91902:CDS 45.0%
!! AAATACGGCAGCGTTTTGGA+TGG - chr5.3:27750340-27750359 MS.gene91902:CDS 45.0%
!! GTAACTGGTAATGGGAACCA+AGG - chr5.3:27750362-27750381 MS.gene91902:CDS 45.0%
AGCTTAGAAACCGGGCTGAA+TGG - chr5.3:27749985-27750004 MS.gene91902:CDS 50.0%
AGTACAACCATCGTTTCCGG+TGG + chr5.3:27749610-27749629 None:intergenic 50.0%
CAACTAGGTTCAGGAATCGG+CGG - chr5.3:27750536-27750555 MS.gene91902:CDS 50.0%
CAGATGGTGAGACGATTGAG+TGG - chr5.3:27749787-27749806 MS.gene91902:CDS 50.0%
CCTGGAAGTCAACTAGGTTC+AGG - chr5.3:27750527-27750546 MS.gene91902:CDS 50.0%
GAGGGAAATTGGGTCTGCTT+CGG + chr5.3:27749524-27749543 None:intergenic 50.0%
GATTGAGTGGCTGCTTCAAC+AGG - chr5.3:27749800-27749819 MS.gene91902:CDS 50.0%
GCTTAGAAACCGGGCTGAAT+GGG - chr5.3:27749986-27750005 MS.gene91902:CDS 50.0%
GGCAGTACAACCATCGTTTC+CGG + chr5.3:27749613-27749632 None:intergenic 50.0%
TGTACAACCACCGGAAACGA+TGG - chr5.3:27749600-27749619 MS.gene91902:CDS 50.0%
! CCTGAACCTAGTTGACTTCC+AGG + chr5.3:27750530-27750549 None:intergenic 50.0%
! TAGTTCTGTCCCATTCAGCC+CGG + chr5.3:27749998-27750017 None:intergenic 50.0%
!! GCAGCGTTTTGGATGGTAAC+TGG - chr5.3:27750347-27750366 MS.gene91902:CDS 50.0%
ACGCGGAGGAGAACGAACTA+GGG + chr5.3:27749959-27749978 None:intergenic 55.0%
ATAGTGAGACGGGGCAGACA+TGG + chr5.3:27749916-27749935 None:intergenic 55.0%
CACACGAAGGTAGATGGACG+AGG - chr5.3:27749693-27749712 MS.gene91902:CDS 55.0%
GATTCGTCGGGACAGCATCA+CGG - chr5.3:27750656-27750675 MS.gene91902:CDS 55.0%
GGCCGGAATTGTTCCTGTTC+CGG + chr5.3:27749850-27749869 None:intergenic 55.0%
GTCCATCTACCTTCGTGTGC+CGG + chr5.3:27749692-27749711 None:intergenic 55.0%
GTTTCTAAGCTGAGCCGACG+CGG + chr5.3:27749976-27749995 None:intergenic 55.0%
TAGTTCGTTCTCCTCCGCGT+CGG - chr5.3:27749959-27749978 MS.gene91902:CDS 55.0%
TTCAAATCCTCCGCCACCAC+CGG + chr5.3:27750192-27750211 None:intergenic 55.0%
! GCCCCGTCTCACTATTTTCG+AGG - chr5.3:27749921-27749940 MS.gene91902:CDS 55.0%
! GTCGGCTCAGCTTAGAAACC+GGG - chr5.3:27749977-27749996 MS.gene91902:CDS 55.0%
!! CGTCGGCTCAGCTTAGAAAC+CGG - chr5.3:27749976-27749995 MS.gene91902:CDS 55.0%
!!! TCGGCGGAGGTTTTTTCTGC+TGG + chr5.3:27749650-27749669 None:intergenic 55.0%
ACGCGTGAGCTGGAACACTC+TGG + chr5.3:27749751-27749770 None:intergenic 60.0%
CGTGAGCTGGAACACTCTGG+CGG + chr5.3:27749748-27749767 None:intergenic 60.0%
CTAGGTTCAGGAATCGGCGG+TGG - chr5.3:27750539-27750558 MS.gene91902:CDS 60.0%
GACCGGCACACGAAGGTAGA+TGG - chr5.3:27749687-27749706 MS.gene91902:CDS 60.0%
GACGCGGAGGAGAACGAACT+AGG + chr5.3:27749960-27749979 None:intergenic 60.0%
GGAGGTGGAGGAGGAGATGA+TGG - chr5.3:27750680-27750699 MS.gene91902:CDS 60.0%
TCCTTCGTCGAAGCGCGCTT+CGG + chr5.3:27749669-27749688 None:intergenic 60.0%
TTCCAGCTCACGCGTGAGCT+GGG - chr5.3:27749756-27749775 MS.gene91902:CDS 60.0%
AAATCCTCCGCCACCACCGG+AGG + chr5.3:27750189-27750208 None:intergenic 65.0%
AGCGCGCTTCGACGAAGGAC+CGG - chr5.3:27749670-27749689 MS.gene91902:CDS 65.0%
AGGTAGATGGACGAGGCCGC+CGG - chr5.3:27749700-27749719 MS.gene91902:CDS 65.0%
CCAGCTGTCATCGCCGCTAC+CGG - chr5.3:27749828-27749847 MS.gene91902:CDS 65.0%
GAACACACTGCCACCGCCTC+CGG - chr5.3:27750170-27750189 MS.gene91902:CDS 65.0%
GACGAAGGACCGGCACACGA+AGG - chr5.3:27749680-27749699 MS.gene91902:CDS 65.0%
GGTTCAGGAATCGGCGGTGG+AGG - chr5.3:27750542-27750561 MS.gene91902:CDS 65.0%
GTCATCGCCGCTACCGGAAC+AGG - chr5.3:27749834-27749853 MS.gene91902:CDS 65.0%
GTTCCAGCTCACGCGTGAGC+TGG - chr5.3:27749755-27749774 MS.gene91902:CDS 65.0%
TCAGGAATCGGCGGTGGAGG+AGG - chr5.3:27750545-27750564 MS.gene91902:CDS 65.0%
TCGTCGGGACAGCATCACGG+CGG - chr5.3:27750659-27750678 MS.gene91902:CDS 65.0%
TCTAAGCTGAGCCGACGCGG+AGG + chr5.3:27749973-27749992 None:intergenic 65.0%
TTCGTCGAAGCGCGCTTCGG+CGG + chr5.3:27749666-27749685 None:intergenic 65.0%
AAGCCGCCGGCATGCGGATC+CGG + chr5.3:27749722-27749741 None:intergenic 70.0%
ACTCTGGCGGCACAAGCCGC+CGG + chr5.3:27749735-27749754 None:intergenic 70.0%
ATCGGCGGTGGAGGAGGAGG+AGG - chr5.3:27750551-27750570 MS.gene91902:CDS 70.0%
CAGCATCACGGCGGAGGTGG+AGG - chr5.3:27750668-27750687 MS.gene91902:CDS 70.0%
CATCACGGCGGAGGTGGAGG+AGG - chr5.3:27750671-27750690 MS.gene91902:CDS 70.0%
GCCGAAGCGCGCTTCGACGA+AGG - chr5.3:27749665-27749684 MS.gene91902:CDS 70.0%
GGAATCGGCGGTGGAGGAGG+AGG - chr5.3:27750548-27750567 MS.gene91902:CDS 70.0%
GGACAGCATCACGGCGGAGG+TGG - chr5.3:27750665-27750684 MS.gene91902:CDS 70.0%
GGTGGAGGAGGAGGAGGTGG+TGG - chr5.3:27750557-27750576 MS.gene91902:CDS 70.0%
GTCCCAGCTCACGCGTGAGC+TGG + chr5.3:27749761-27749780 None:intergenic 70.0%
TCGGGACAGCATCACGGCGG+AGG - chr5.3:27750662-27750681 MS.gene91902:CDS 70.0%
! CCGGTAGCGGCGATGACAGC+TGG + chr5.3:27749831-27749850 None:intergenic 70.0%
ACTGCCACCGCCTCCGGTGG+TGG - chr5.3:27750176-27750195 MS.gene91902:CDS 75.0%
AGGCCGCCGGATCCGCATGC+CGG - chr5.3:27749713-27749732 MS.gene91902:CDS 75.0%
CACACTGCCACCGCCTCCGG+TGG - chr5.3:27750173-27750192 MS.gene91902:CDS 75.0%
CGGCACAAGCCGCCGGCATG+CGG + chr5.3:27749728-27749747 None:intergenic 75.0%
GGCGGTGGAGGAGGAGGAGG+TGG - chr5.3:27750554-27750573 MS.gene91902:CDS 75.0%
GTCGAAGCGCGCTTCGGCGG+AGG + chr5.3:27749663-27749682 None:intergenic 75.0%
TCCTCCGCCACCACCGGAGG+CGG + chr5.3:27750186-27750205 None:intergenic 75.0%
! CCGCCGGATCCGCATGCCGG+CGG - chr5.3:27749716-27749735 MS.gene91902:CDS 80.0%
! CCGCCGGCATGCGGATCCGG+CGG + chr5.3:27749719-27749738 None:intergenic 80.0%
! TCCGCCACCACCGGAGGCGG+TGG + chr5.3:27750183-27750202 None:intergenic 80.0%
!! GCCACCGCCTCCGGTGGTGG+CGG - chr5.3:27750179-27750198 MS.gene91902:CDS 80.0%
!!! ACCGCCTCCGGTGGTGGCGG+AGG - chr5.3:27750182-27750201 MS.gene91902:CDS 80.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 27749483 27750730 27749483 ID=MS.gene91902
chr5.3 mRNA 27749483 27750730 27749483 ID=MS.gene91902.t1;Parent=MS.gene91902
chr5.3 exon 27749483 27750730 27749483 ID=MS.gene91902.t1.exon1;Parent=MS.gene91902.t1
chr5.3 CDS 27749483 27750730 27749483 ID=cds.MS.gene91902.t1;Parent=MS.gene91902.t1
Gene Sequence

>MS.gene91902

ATGGAATTTGAAGGTGCTCATCATCAAAATAATGGAATCCGAAGCAGACCCAATTTCCCTCTTCAGCTTTTAGAGAAAAAAGACGACAACATCCTAGATGTCTCAGAAGAACAAACTTGTACAACCACCGGAAACGATGGTTGTACTGCCATAATCTCATCAGACCAGCAGAAAAAACCTCCGCCGAAGCGCGCTTCGACGAAGGACCGGCACACGAAGGTAGATGGACGAGGCCGCCGGATCCGCATGCCGGCGGCTTGTGCCGCCAGAGTGTTCCAGCTCACGCGTGAGCTGGGACATAAATCAGATGGTGAGACGATTGAGTGGCTGCTTCAACAGGCAGAACCAGCTGTCATCGCCGCTACCGGAACAGGAACAATTCCGGCCAACTTTACTTCACTTAATATATCTCTTAGAAGCTCAGGCTCAACCATGTCTGCCCCGTCTCACTATTTTCGAGGTAACTACTTTAACCCTAGTTCGTTCTCCTCCGCGTCGGCTCAGCTTAGAAACCGGGCTGAATGGGACAGAACTATGAACATGAACATGAACATGAACGTTGTAGCCGATGATTCTAGAAGGAGTAGTAACTCTACCATGTTGGAAAATCCTTCTTCTTTATCAGCAATGTTGAATTTCAACAACGTGCAAAATGCTATTCTACAAGCAAAACAAGAGCTTCGGGAAGAACACACTGCCACCGCCTCCGGTGGTGGCGGAGGATTTGAATTGTTAGGTTCAGATTCAGATGGAAGTATAGGGAGGAAAAGAAGGTCAGAGCAAGAATTATTATCACAAAATATGGGAAGTTATTTGTTACAATCAAATGTAGGGTCACTTCCTTCAAGTCATGCTTCAAATACGGCAGCGTTTTGGATGGTAACTGGTAATGGGAACCAAGGTGTTAATGTGAATGGTGGTAATGGAAATGATCCAATTTGGGCAATTCCTTCTGTTGGAAACAATGGTTTATATAGAGGAGCTATGTCATCTCCTAATGGATTTCATTTCATGAATTTTGCTGCATCTCCAATGAATCTTATGCCTGGAAGTCAACTAGGTTCAGGAATCGGCGGTGGAGGAGGAGGAGGTGGTGGTATTTTAGCCGAAAGCAATCTGAGTATGCTTTCGGCTATGAATGCCTATAGGCAGATTTCGACGAATGGTATGTCAGATTCGTCGGGACAGCATCACGGCGGAGGTGGAGGAGGAGATGATGGACATGACTCAAGCAGTCAGCATTCCTAA
Protein sequence

>MS.gene91902.t1

MEFEGAHHQNNGIRSRPNFPLQLLEKKDDNILDVSEEQTCTTTGNDGCTAIISSDQQKKPPPKRASTKDRHTKVDGRGRRIRMPAACAARVFQLTRELGHKSDGETIEWLLQQAEPAVIAATGTGTIPANFTSLNISLRSSGSTMSAPSHYFRGNYFNPSSFSSASAQLRNRAEWDRTMNMNMNMNVVADDSRRSSNSTMLENPSSLSAMLNFNNVQNAILQAKQELREEHTATASGGGGGFELLGSDSDGSIGRKRRSEQELLSQNMGSYLLQSNVGSLPSSHASNTAAFWMVTGNGNQGVNVNGGNGNDPIWAIPSVGNNGLYRGAMSSPNGFHFMNFAASPMNLMPGSQLGSGIGGGGGGGGGILAESNLSMLSAMNAYRQISTNGMSDSSGQHHGGGGGGDDGHDSSSQHS