Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91969.t1 | XP_003618014.1 | 96.4 | 56 | 2 | 0 | 1 | 56 | 1 | 56 | 2.40E-15 | 92.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91969.t1 | G7K500 | 96.4 | 56 | 2 | 0 | 1 | 56 | 1 | 56 | 1.8e-15 | 92.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050648 | MS.gene91969 | 0.803352 | 3.63E-49 | -1.69E-46 |
| MS.gene051648 | MS.gene91969 | 0.806892 | 6.60E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91969.t1 | MTR_5g098020 | 91.282 | 195 | 17 | 0 | 1 | 195 | 1 | 195 | 3.99e-79 | 234 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 39 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGCTGAAGATTCTTCTT+TGG | 0.248746 | 5.2:+82507811 | None:intergenic |
| TTGGACTCTCAACGATTTCT+TGG | 0.283330 | 5.2:+82507855 | None:intergenic |
| TTCTGTTGTTGAGGCTGTTC+AGG | 0.288375 | 5.2:-82507609 | MS.gene91969:CDS |
| TCTGATGCTGCTGGTGTGAT+TGG | 0.302513 | 5.2:+82507836 | None:intergenic |
| GTCGAGGAAACAAAAGAAAA+TGG | 0.336894 | 5.2:-82507635 | MS.gene91969:CDS |
| CTCAACAGGTTCCTTTGTCT+CGG | 0.374352 | 5.2:+82507688 | None:intergenic |
| TCTGTCTCAGATTCTGCAGC+TGG | 0.400444 | 5.2:+82507581 | None:intergenic |
| CTCAGCTTTAACCAAAGGTT+TGG | 0.405277 | 5.2:+82507784 | None:intergenic |
| TGGACTCTCAACGATTTCTT+GGG | 0.409670 | 5.2:+82507856 | None:intergenic |
| CCTTACTCACTCTTCTCAGT+TGG | 0.426357 | 5.2:+82507374 | None:intergenic |
| AGCTGAAGAAGCCAAACCTT+TGG | 0.430085 | 5.2:-82507795 | MS.gene91969:CDS |
| TGAGACAGAAAATGTTGTTA+AGG | 0.433126 | 5.2:-82507567 | MS.gene91969:CDS |
| GGTAGCTGAGGCTGAAACTA+AGG | 0.438659 | 5.2:-82507534 | MS.gene91969:CDS |
| CCAACTGAGAAGAGTGAGTA+AGG | 0.442677 | 5.2:-82507374 | None:intergenic |
| GGTTGAGAAAGAAGAAGCCT+TGG | 0.446199 | 5.2:-82507465 | MS.gene91969:CDS |
| TCTTTGGTTTCTGATGCTGC+TGG | 0.462673 | 5.2:+82507827 | None:intergenic |
| TCCAATTATGGCCACTGTTG+AGG | 0.502667 | 5.2:-82508670 | None:intergenic |
| TACAGCAGAGGAAACAAAGA+AGG | 0.515632 | 5.2:-82507720 | MS.gene91969:CDS |
| AATTGAGAAGAGTGAAGAAG+AGG | 0.516526 | 5.2:-82507399 | MS.gene91969:CDS |
| GGTTAAAGCTGAGGCAAAAG+AGG | 0.520787 | 5.2:-82507774 | MS.gene91969:CDS |
| GGAAGAACAAACCGAGACAA+AGG | 0.535864 | 5.2:-82507699 | MS.gene91969:CDS |
| TTTGCCTCAGCTTTAACCAA+AGG | 0.538136 | 5.2:+82507779 | None:intergenic |
| GTTGAGGAAACAAAAGAAAA+CGG | 0.538839 | 5.2:-82507671 | MS.gene91969:CDS |
| AGAAGAGAAAAGTGAGGAGA+AGG | 0.539879 | 5.2:-82507486 | MS.gene91969:CDS |
| ACTCTCAACGATTTCTTGGG+TGG | 0.555409 | 5.2:+82507859 | None:intergenic |
| AGCCTTGGATGAAAAGAAAG+AGG | 0.562181 | 5.2:-82507450 | MS.gene91969:CDS |
| CGAGACAAAGGAACCTGTTG+AGG | 0.574462 | 5.2:-82507687 | MS.gene91969:CDS |
| CAAACCTTTGGTTAAAGCTG+AGG | 0.582591 | 5.2:-82507783 | MS.gene91969:CDS |
| AAATGGTGATTCTGTTGTTG+AGG | 0.613322 | 5.2:-82507618 | MS.gene91969:CDS |
| TGTTGTTAAGGATGAGAATG+TGG | 0.621253 | 5.2:-82507555 | MS.gene91969:CDS |
| CGGTGATTCAGTTAATGTCG+AGG | 0.622446 | 5.2:-82507651 | MS.gene91969:CDS |
| TGATAACTGAGCTGTGTCGT+CGG | 0.632470 | 5.2:+82507901 | None:intergenic |
| CAACTGAGAAGAGTGAGTAA+GGG | 0.654993 | 5.2:-82507373 | None:intergenic |
| AGCCTCAGAAGAGAAAAGTG+AGG | 0.660719 | 5.2:-82507492 | MS.gene91969:CDS |
| GGATGAGAATGTGGTAGCTG+AGG | 0.670593 | 5.2:-82507546 | MS.gene91969:CDS |
| GGTGGTAGAAGAAGTTCTTG+TGG | 0.675484 | 5.2:-82507753 | MS.gene91969:CDS |
| TAAAGCTGAGGCAAAAGAGG+TGG | 0.676484 | 5.2:-82507771 | MS.gene91969:CDS |
| GGCTGTGAAAGATACAGCAG+AGG | 0.684792 | 5.2:-82507732 | MS.gene91969:CDS |
| TGGTAGAGTTACCTCAACAG+TGG | 0.712754 | 5.2:+82508659 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGGAAATATTAATTATAAT+TGG | - | chr5.2:82507771-82507790 | MS.gene91969:CDS | 10.0% |
| !!! | AATCAGTGTTTAAAATAAAA+TGG | + | chr5.2:82507452-82507471 | None:intergenic | 15.0% |
| !!! | AATTTTGAGTCTTTTATTTT+TGG | - | chr5.2:82507877-82507896 | MS.gene91969:CDS | 15.0% |
| !!! | AGATATTTTTTTCTTATGAA+CGG | + | chr5.2:82507914-82507933 | None:intergenic | 15.0% |
| !!! | ATCAGTGTTTAAAATAAAAT+GGG | + | chr5.2:82507451-82507470 | None:intergenic | 15.0% |
| !! | AAAATTATGATGAAAAACCA+AGG | + | chr5.2:82507415-82507434 | None:intergenic | 20.0% |
| !! | AGTAAAATCTGAACAAAAAT+TGG | - | chr5.2:82507963-82507982 | MS.gene91969:intron | 20.0% |
| !!! | AAAATTTGAAGCTCTTAAAA+TGG | - | chr5.2:82507752-82507771 | MS.gene91969:CDS | 20.0% |
| !!! | GATATTTTTTTCTTATGAAC+GGG | + | chr5.2:82507913-82507932 | None:intergenic | 20.0% |
| ! | ACATGACATGTTATTAATCA+CGG | - | chr5.2:82507549-82507568 | MS.gene91969:CDS | 25.0% |
| ! | TCATATCTACTTGAGTAAAT+AGG | + | chr5.2:82507724-82507743 | None:intergenic | 25.0% |
| ! | TGATTAATAACATGTCATGT+AGG | + | chr5.2:82507549-82507568 | None:intergenic | 25.0% |
| !! | CATCAATACTTTTATGTCTT+TGG | + | chr5.2:82507655-82507674 | None:intergenic | 25.0% |
| !!! | AATATTTTGAAGCATCTAGT+AGG | - | chr5.2:82507486-82507505 | MS.gene91969:CDS | 25.0% |
| !!! | ACCTTTTTTAATGCATTAGA+TGG | - | chr5.2:82507675-82507694 | MS.gene91969:CDS | 25.0% |
| AAAAACCAAGGTCTTGAAAT+TGG | + | chr5.2:82507403-82507422 | None:intergenic | 30.0% | |
| ATCTACTTGAGTAAATAGGA+AGG | + | chr5.2:82507720-82507739 | None:intergenic | 30.0% | |
| GACACATTGTTTAGTAAAAC+CGG | + | chr5.2:82508008-82508027 | None:intergenic | 30.0% | |
| GCCATCTAATGCATTAAAAA+AGG | + | chr5.2:82507679-82507698 | None:intergenic | 30.0% | |
| GTTGAGGAAACAAAAGAAAA+CGG | - | chr5.2:82508368-82508387 | MS.gene91969:intron | 30.0% | |
| TGAGACAGAAAATGTTGTTA+AGG | - | chr5.2:82508472-82508491 | MS.gene91969:intron | 30.0% | |
| TGTGCATGAATTAATTCATG+TGG | + | chr5.2:82507815-82507834 | None:intergenic | 30.0% | |
| ! | CTCAATTTTTGCATCATTGT+TGG | + | chr5.2:82508627-82508646 | None:intergenic | 30.0% |
| !! | TTTTCTTTTGTTTCCTCAAC+AGG | + | chr5.2:82508368-82508387 | None:intergenic | 30.0% |
| !!! | TTTTTTTCTTATGAACGGGA+CGG | + | chr5.2:82507909-82507928 | None:intergenic | 30.0% |
| AAATGGTGATTCTGTTGTTG+AGG | - | chr5.2:82508421-82508440 | MS.gene91969:intron | 35.0% | |
| AATTGAGAAGAGTGAAGAAG+AGG | - | chr5.2:82508640-82508659 | MS.gene91969:intron | 35.0% | |
| ACTCTGACTAATATGACACA+TGG | + | chr5.2:82507842-82507861 | None:intergenic | 35.0% | |
| ATGTCATGTAGGAATAATGC+AGG | + | chr5.2:82507538-82507557 | None:intergenic | 35.0% | |
| GTCGAGGAAACAAAAGAAAA+TGG | - | chr5.2:82508404-82508423 | MS.gene91969:intron | 35.0% | |
| TGATCTGAATATGATTCTGC+AGG | - | chr5.2:82508091-82508110 | MS.gene91969:intron | 35.0% | |
| TGTCATGTAGGAATAATGCA+GGG | + | chr5.2:82507537-82507556 | None:intergenic | 35.0% | |
| TGTTGTTAAGGATGAGAATG+TGG | - | chr5.2:82508484-82508503 | MS.gene91969:intron | 35.0% | |
| TTCAGATAAGATTCGAACTC+CGG | - | chr5.2:82507986-82508005 | MS.gene91969:intron | 35.0% | |
| TTCAGCTGAAGATTCTTCTT+TGG | + | chr5.2:82508231-82508250 | None:intergenic | 35.0% | |
| ! | TTCCTCTTTCTTTTCATCCA+AGG | + | chr5.2:82508594-82508613 | None:intergenic | 35.0% |
| !! | GTAAACTCTGAAGTTCGATT+AGG | - | chr5.2:82507935-82507954 | MS.gene91969:intron | 35.0% |
| AGAAGAGAAAAGTGAGGAGA+AGG | - | chr5.2:82508553-82508572 | MS.gene91969:intron | 40.0% | |
| AGCCTTGGATGAAAAGAAAG+AGG | - | chr5.2:82508589-82508608 | MS.gene91969:intron | 40.0% | |
| CAAACCTTTGGTTAAAGCTG+AGG | - | chr5.2:82508256-82508275 | MS.gene91969:intron | 40.0% | |
| CTCTACCAATTTCAAGACCT+TGG | - | chr5.2:82507395-82507414 | MS.gene91969:CDS | 40.0% | |
| TACAGCAGAGGAAACAAAGA+AGG | - | chr5.2:82508319-82508338 | MS.gene91969:intron | 40.0% | |
| TGGACTCTCAACGATTTCTT+GGG | + | chr5.2:82508186-82508205 | None:intergenic | 40.0% | |
| TTGGACTCTCAACGATTTCT+TGG | + | chr5.2:82508187-82508206 | None:intergenic | 40.0% | |
| ! | CTCAGCTTTAACCAAAGGTT+TGG | + | chr5.2:82508258-82508277 | None:intergenic | 40.0% |
| ! | TTTGCCTCAGCTTTAACCAA+AGG | + | chr5.2:82508263-82508282 | None:intergenic | 40.0% |
| !! | AACTCTGAAGTTCGATTAGG+AGG | - | chr5.2:82507938-82507957 | MS.gene91969:intron | 40.0% |
| ACTCTCAACGATTTCTTGGG+TGG | + | chr5.2:82508183-82508202 | None:intergenic | 45.0% | |
| AGCCTCAGAAGAGAAAAGTG+AGG | - | chr5.2:82508547-82508566 | MS.gene91969:intron | 45.0% | |
| AGCTGAAGAAGCCAAACCTT+TGG | - | chr5.2:82508244-82508263 | MS.gene91969:intron | 45.0% | |
| CGGTGATTCAGTTAATGTCG+AGG | - | chr5.2:82508388-82508407 | MS.gene91969:intron | 45.0% | |
| CTCAACAGGTTCCTTTGTCT+CGG | + | chr5.2:82508354-82508373 | None:intergenic | 45.0% | |
| GGAAGAACAAACCGAGACAA+AGG | - | chr5.2:82508340-82508359 | MS.gene91969:intron | 45.0% | |
| GGTGGTAGAAGAAGTTCTTG+TGG | - | chr5.2:82508286-82508305 | MS.gene91969:intron | 45.0% | |
| GGTTAAAGCTGAGGCAAAAG+AGG | - | chr5.2:82508265-82508284 | MS.gene91969:intron | 45.0% | |
| GGTTGAGAAAGAAGAAGCCT+TGG | - | chr5.2:82508574-82508593 | MS.gene91969:intron | 45.0% | |
| TAAAGCTGAGGCAAAAGAGG+TGG | - | chr5.2:82508268-82508287 | MS.gene91969:intron | 45.0% | |
| TGATAACTGAGCTGTGTCGT+CGG | + | chr5.2:82508141-82508160 | None:intergenic | 45.0% | |
| TGGTAGAGTTACCTCAACAG+TGG | + | chr5.2:82507383-82507402 | None:intergenic | 45.0% | |
| TTCTGTTGTTGAGGCTGTTC+AGG | - | chr5.2:82508430-82508449 | MS.gene91969:intron | 45.0% | |
| ! | TCTTTGGTTTCTGATGCTGC+TGG | + | chr5.2:82508215-82508234 | None:intergenic | 45.0% |
| !! | CTCCTCACTTTTCTCTTCTG+AGG | + | chr5.2:82508552-82508571 | None:intergenic | 45.0% |
| CGAGACAAAGGAACCTGTTG+AGG | - | chr5.2:82508352-82508371 | MS.gene91969:intron | 50.0% | |
| CGTGACGCGATTTGAACTAC+AGG | + | chr5.2:82507626-82507645 | None:intergenic | 50.0% | |
| GGATGAGAATGTGGTAGCTG+AGG | - | chr5.2:82508493-82508512 | MS.gene91969:intron | 50.0% | |
| GGCTGTGAAAGATACAGCAG+AGG | - | chr5.2:82508307-82508326 | MS.gene91969:intron | 50.0% | |
| GGTAGCTGAGGCTGAAACTA+AGG | - | chr5.2:82508505-82508524 | MS.gene91969:intron | 50.0% | |
| TCTGATGCTGCTGGTGTGAT+TGG | + | chr5.2:82508206-82508225 | None:intergenic | 50.0% | |
| TCTGTCTCAGATTCTGCAGC+TGG | + | chr5.2:82508461-82508480 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 82507376 | 82508685 | 82507376 | ID=MS.gene91969 |
| chr5.2 | mRNA | 82507376 | 82508685 | 82507376 | ID=MS.gene91969.t1;Parent=MS.gene91969 |
| chr5.2 | exon | 82508671 | 82508685 | 82508671 | ID=MS.gene91969.t1.exon1;Parent=MS.gene91969.t1 |
| chr5.2 | CDS | 82508671 | 82508685 | 82508671 | ID=cds.MS.gene91969.t1;Parent=MS.gene91969.t1 |
| chr5.2 | exon | 82507376 | 82507948 | 82507376 | ID=MS.gene91969.t1.exon2;Parent=MS.gene91969.t1 |
| chr5.2 | CDS | 82507376 | 82507948 | 82507376 | ID=cds.MS.gene91969.t1;Parent=MS.gene91969.t1 |
| Gene Sequence |
| Protein sequence |
