Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92024.t1 | XP_013447725.1 | 98.7 | 228 | 3 | 0 | 1 | 228 | 1 | 228 | 1.30E-120 | 442.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92024.t1 | A0A072U7J6 | 98.7 | 228 | 3 | 0 | 1 | 228 | 1 | 228 | 9.0e-121 | 442.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050959 | MS.gene92024 | 0.813208 | 2.87E-51 | -1.69E-46 |
| MS.gene058168 | MS.gene92024 | 0.800968 | 1.12E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene30204 | MS.gene92024 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92024.t1 | MTR_7g018370 | 98.760 | 242 | 3 | 0 | 1 | 242 | 1 | 242 | 1.83e-176 | 488 |
| MS.gene92024.t1 | MTR_6g017240 | 68.750 | 240 | 66 | 5 | 9 | 242 | 11 | 247 | 1.05e-106 | 311 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92024.t1 | AT5G16030 | 55.556 | 252 | 85 | 9 | 14 | 242 | 4 | 251 | 3.90e-73 | 224 |
| MS.gene92024.t1 | AT5G16030 | 55.556 | 252 | 85 | 9 | 14 | 242 | 4 | 251 | 1.15e-71 | 223 |
| MS.gene92024.t1 | AT5G16030 | 53.846 | 260 | 85 | 9 | 14 | 242 | 4 | 259 | 2.58e-70 | 219 |
| MS.gene92024.t1 | AT5G16030 | 55.336 | 253 | 85 | 10 | 14 | 242 | 4 | 252 | 5.72e-70 | 219 |
| MS.gene92024.t1 | AT3G02500 | 49.587 | 242 | 97 | 9 | 14 | 242 | 4 | 233 | 2.82e-61 | 195 |
| MS.gene92024.t1 | AT3G02500 | 48.971 | 243 | 98 | 9 | 14 | 242 | 4 | 234 | 6.40e-61 | 194 |
| MS.gene92024.t1 | AT3G02500 | 45.041 | 242 | 93 | 10 | 14 | 242 | 4 | 218 | 1.71e-48 | 161 |
| MS.gene92024.t1 | AT5G16030 | 52.761 | 163 | 56 | 6 | 98 | 242 | 2 | 161 | 2.17e-38 | 134 |
Find 47 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGTAATGTACCTTTAAA+AGG | 0.181978 | 7.3:-79990694 | None:intergenic |
| TCTTTCACCACTGCCCTTAA+AGG | 0.188324 | 7.3:+79990825 | MS.gene92024:CDS |
| TATATATGCAATCCACTTTC+AGG | 0.234776 | 7.3:+79991226 | MS.gene92024:CDS |
| GAATTTAATGCAAACCCTTC+TGG | 0.264923 | 7.3:-79991181 | None:intergenic |
| ATCTCTTCTTCTTGAACTAT+AGG | 0.274819 | 7.3:-79990503 | None:intergenic |
| TCTCTTCTTCTTGAACTATA+GGG | 0.297403 | 7.3:-79990502 | None:intergenic |
| AAAATTAATATTACCTTTAA+GGG | 0.307677 | 7.3:-79990838 | None:intergenic |
| TCTCTTAAATTTCCAATTCC+AGG | 0.312931 | 7.3:+79991397 | MS.gene92024:CDS |
| AACAATTCTAGAAGGTATTC+AGG | 0.354558 | 7.3:+79990586 | MS.gene92024:CDS |
| AAAGCATATGAACCTGGAAT+TGG | 0.366715 | 7.3:-79991409 | None:intergenic |
| TCTTCATGTGTGTAAATTGA+TGG | 0.384210 | 7.3:-79991370 | None:intergenic |
| GGGATATGATTCAGCATTGT+GGG | 0.444974 | 7.3:-79990482 | None:intergenic |
| TGAAGAAGCAGTACTAGAAA+TGG | 0.463289 | 7.3:-79990662 | None:intergenic |
| AATATTACCTTTAAGGGCAG+TGG | 0.469379 | 7.3:-79990832 | None:intergenic |
| AGAGTAACAACAATTCTAGA+AGG | 0.470000 | 7.3:+79990578 | MS.gene92024:CDS |
| TCTAGTACTGCTTCTTCACC+TGG | 0.472912 | 7.3:+79990667 | MS.gene92024:CDS |
| ATGATGGATGGAGTTGAAGT+AGG | 0.491118 | 7.3:-79991812 | None:intergenic |
| ATTGGTACCTCTCCTGAAAG+TGG | 0.491756 | 7.3:-79991238 | None:intergenic |
| CAAGGGTAAGCTATAACAGT+TGG | 0.503145 | 7.3:+79991146 | MS.gene92024:CDS |
| TTTGCTGATAAACATTCCAT+TGG | 0.503270 | 7.3:-79991256 | None:intergenic |
| AAAGTAATGTACCTTTAAAA+GGG | 0.508301 | 7.3:-79990693 | None:intergenic |
| AGAGGGAATGACAAGAGATG+TGG | 0.508546 | 7.3:+79991745 | MS.gene92024:CDS |
| GTACCTTTAAAAGGGTATCC+AGG | 0.521359 | 7.3:-79990685 | None:intergenic |
| TTTAACCTCTCTATGATGGA+TGG | 0.523140 | 7.3:-79991824 | None:intergenic |
| GTGGTGAAAGAATATGTTGA+AGG | 0.526710 | 7.3:-79990813 | None:intergenic |
| TTCATACAGCATTACAAGCA+AGG | 0.534612 | 7.3:+79991128 | MS.gene92024:intron |
| CTATGCAGAAAAGAAGAAAG+AGG | 0.536699 | 7.3:+79991727 | MS.gene92024:intron |
| TGATGGATGGAGTTGAAGTA+GGG | 0.547063 | 7.3:-79991811 | None:intergenic |
| AAGGGTAAGCTATAACAGTT+GGG | 0.551130 | 7.3:+79991147 | MS.gene92024:CDS |
| GATGGAGTTGAAGTAGGGCT+AGG | 0.557032 | 7.3:-79991806 | None:intergenic |
| TGGGAGTGTTTATCTCCAGA+AGG | 0.557249 | 7.3:+79991166 | MS.gene92024:CDS |
| GAGGGAATGACAAGAGATGT+GGG | 0.562323 | 7.3:+79991746 | MS.gene92024:CDS |
| TTTGCATTTGAATTTGAACT+AGG | 0.572177 | 7.3:-79991866 | None:intergenic |
| GGACTGCTTGAACTAAGATA+AGG | 0.574582 | 7.3:-79991785 | None:intergenic |
| AGGGATATGATTCAGCATTG+TGG | 0.580036 | 7.3:-79990483 | None:intergenic |
| TCATACAGCATTACAAGCAA+GGG | 0.583811 | 7.3:+79991129 | MS.gene92024:intron |
| GGGAGTGTTTATCTCCAGAA+GGG | 0.586530 | 7.3:+79991167 | MS.gene92024:CDS |
| TATGCAGAAAAGAAGAAAGA+GGG | 0.610950 | 7.3:+79991728 | MS.gene92024:intron |
| CTGCTTGAACTAAGATAAGG+AGG | 0.612198 | 7.3:-79991782 | None:intergenic |
| TGTCTTGCAGAATAAACAAG+AGG | 0.624414 | 7.3:-79991334 | None:intergenic |
| GGTTTGCATTAAATTCAAAG+TGG | 0.634839 | 7.3:+79991188 | MS.gene92024:CDS |
| TGGTGAAAGAATATGTTGAA+GGG | 0.636354 | 7.3:-79990812 | None:intergenic |
| ACTTTCAGGAGAGGTACCAA+TGG | 0.645021 | 7.3:+79991240 | MS.gene92024:CDS |
| CAACTCCATCCATCATAGAG+AGG | 0.653040 | 7.3:+79991819 | MS.gene92024:CDS |
| ATGCAATCCACTTTCAGGAG+AGG | 0.654107 | 7.3:+79991231 | MS.gene92024:CDS |
| GAAGCTTTAGAGAAGATACA+AGG | 0.659098 | 7.3:+79990620 | MS.gene92024:CDS |
| AGGGAATGACAAGAGATGTG+GGG | 0.701802 | 7.3:+79991747 | MS.gene92024:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATTAATATTACCTTTA+AGG | - | chr7.3:79990842-79990861 | None:intergenic | 10.0% |
| !! | AAAATTAATATTACCTTTAA+GGG | - | chr7.3:79990841-79990860 | None:intergenic | 10.0% |
| !! | TATATTTATTGAGAAAAATT+TGG | - | chr7.3:79991918-79991937 | None:intergenic | 10.0% |
| !! | AATAAGCTAAAAACAATTTA+TGG | - | chr7.3:79991548-79991567 | None:intergenic | 15.0% |
| !! | TTAATTTATCCAAAAAGTTT+AGG | - | chr7.3:79991100-79991119 | None:intergenic | 15.0% |
| !!! | AACTTTTTGGATAAATTAAA+TGG | + | chr7.3:79991101-79991120 | MS.gene92024:intron | 15.0% |
| !! | AAAAGTAATGTACCTTTAAA+AGG | - | chr7.3:79990697-79990716 | None:intergenic | 20.0% |
| !! | AACAGATACTAAAATGTTTA+AGG | - | chr7.3:79991989-79992008 | None:intergenic | 20.0% |
| !! | TATATAGTTCATGAATTTCA+TGG | + | chr7.3:79990735-79990754 | MS.gene92024:intron | 20.0% |
| !!! | AAAGTAATGTACCTTTAAAA+GGG | - | chr7.3:79990696-79990715 | None:intergenic | 20.0% |
| !!! | AATTGTTTTTAGCTTATTTC+TGG | + | chr7.3:79991550-79991569 | MS.gene92024:intron | 20.0% |
| !!! | AGAATCATGGTTTTAAATTA+CGG | + | chr7.3:79990915-79990934 | MS.gene92024:intron | 20.0% |
| !!! | CTATATAGTTTGAGTTAAAA+TGG | - | chr7.3:79990722-79990741 | None:intergenic | 20.0% |
| ! | AAAAGGCGAAAGAATAATAA+AGG | + | chr7.3:79990871-79990890 | MS.gene92024:intron | 25.0% |
| ! | AAATGCAAAAACAAAATCTG+AGG | + | chr7.3:79991880-79991899 | MS.gene92024:CDS | 25.0% |
| ! | CCTCAAAATTCTTTATATTG+CGG | + | chr7.3:79991005-79991024 | MS.gene92024:intron | 25.0% |
| ! | TTTGATTCCTAATTCTACTT+CGG | + | chr7.3:79991430-79991449 | MS.gene92024:intron | 25.0% |
| ! | TTTGCATTTGAATTTGAACT+AGG | - | chr7.3:79991869-79991888 | None:intergenic | 25.0% |
| !!! | CAGATTTTAATTTGAGAGAA+TGG | + | chr7.3:79990530-79990549 | MS.gene92024:CDS | 25.0% |
| !!! | TATAAAGAATTTTGAGGTCT+CGG | - | chr7.3:79991002-79991021 | None:intergenic | 25.0% |
| !!! | TATTTTTGGCCTAAACTTTT+TGG | + | chr7.3:79991088-79991107 | MS.gene92024:intron | 25.0% |
| AAAAACAAAATCTGAGGAAG+AGG | + | chr7.3:79991886-79991905 | MS.gene92024:CDS | 30.0% | |
| AGAGTAACAACAATTCTAGA+AGG | + | chr7.3:79990578-79990597 | MS.gene92024:CDS | 30.0% | |
| ATCTCTTCTTCTTGAACTAT+AGG | - | chr7.3:79990506-79990525 | None:intergenic | 30.0% | |
| CACATTGTTAACTAGAATCA+TGG | + | chr7.3:79990902-79990921 | MS.gene92024:intron | 30.0% | |
| GGTTTGCATTAAATTCAAAG+TGG | + | chr7.3:79991188-79991207 | MS.gene92024:CDS | 30.0% | |
| TAAGTAAGTCAATCTGAACA+TGG | - | chr7.3:79991456-79991475 | None:intergenic | 30.0% | |
| TATATATGCAATCCACTTTC+AGG | + | chr7.3:79991226-79991245 | MS.gene92024:CDS | 30.0% | |
| TATGCAGAAAAGAAGAAAGA+GGG | + | chr7.3:79991728-79991747 | MS.gene92024:intron | 30.0% | |
| TCTCTTAAATTTCCAATTCC+AGG | + | chr7.3:79991397-79991416 | MS.gene92024:CDS | 30.0% | |
| TCTCTTCTTCTTGAACTATA+GGG | - | chr7.3:79990505-79990524 | None:intergenic | 30.0% | |
| TCTTCATGTGTGTAAATTGA+TGG | - | chr7.3:79991373-79991392 | None:intergenic | 30.0% | |
| TGGTGAAAGAATATGTTGAA+GGG | - | chr7.3:79990815-79990834 | None:intergenic | 30.0% | |
| TTATCAGCAAAAACTCTTAG+TGG | + | chr7.3:79991268-79991287 | MS.gene92024:CDS | 30.0% | |
| TTTGCTGATAAACATTCCAT+TGG | - | chr7.3:79991259-79991278 | None:intergenic | 30.0% | |
| ! | CATGATTCTAGTTAACAATG+TGG | - | chr7.3:79990904-79990923 | None:intergenic | 30.0% |
| ! | CCGCAATATAAAGAATTTTG+AGG | - | chr7.3:79991008-79991027 | None:intergenic | 30.0% |
| !! | AACAATTCTAGAAGGTATTC+AGG | + | chr7.3:79990586-79990605 | MS.gene92024:CDS | 30.0% |
| AAAGCATATGAACCTGGAAT+TGG | - | chr7.3:79991412-79991431 | None:intergenic | 35.0% | |
| AAGGGTAAGCTATAACAGTT+GGG | + | chr7.3:79991147-79991166 | MS.gene92024:CDS | 35.0% | |
| CTATGCAGAAAAGAAGAAAG+AGG | + | chr7.3:79991727-79991746 | MS.gene92024:intron | 35.0% | |
| GAATTTAATGCAAACCCTTC+TGG | - | chr7.3:79991184-79991203 | None:intergenic | 35.0% | |
| GTGGTGAAAGAATATGTTGA+AGG | - | chr7.3:79990816-79990835 | None:intergenic | 35.0% | |
| TAAGCTTGCAATTCTAGTCA+TGG | - | chr7.3:79991051-79991070 | None:intergenic | 35.0% | |
| TCATACAGCATTACAAGCAA+GGG | + | chr7.3:79991129-79991148 | MS.gene92024:intron | 35.0% | |
| TGAAGAAGCAGTACTAGAAA+TGG | - | chr7.3:79990665-79990684 | None:intergenic | 35.0% | |
| TGTCTTGCAGAATAAACAAG+AGG | - | chr7.3:79991337-79991356 | None:intergenic | 35.0% | |
| TTCATACAGCATTACAAGCA+AGG | + | chr7.3:79991128-79991147 | MS.gene92024:intron | 35.0% | |
| ! | AATATTACCTTTAAGGGCAG+TGG | - | chr7.3:79990835-79990854 | None:intergenic | 35.0% |
| ! | AGCTTTTAACCTCTCTATGA+TGG | - | chr7.3:79991831-79991850 | None:intergenic | 35.0% |
| ! | GAATCAAAAGCATATGAACC+TGG | - | chr7.3:79991418-79991437 | None:intergenic | 35.0% |
| ! | GTTTTTACTGTGTATGTGAG+TGG | - | chr7.3:79992037-79992056 | None:intergenic | 35.0% |
| ! | TTTAACCTCTCTATGATGGA+TGG | - | chr7.3:79991827-79991846 | None:intergenic | 35.0% |
| !! | GAAGCTTTAGAGAAGATACA+AGG | + | chr7.3:79990620-79990639 | MS.gene92024:CDS | 35.0% |
| !! | GTTGTTTTCGTAAGCTATTC+TGG | - | chr7.3:79991590-79991609 | None:intergenic | 35.0% |
| !! | TTTAGCTTATTTCTGGTAGC+CGG | + | chr7.3:79991557-79991576 | MS.gene92024:intron | 35.0% |
| !!! | ATGGTTTTAAATTACGGTCG+CGG | + | chr7.3:79990921-79990940 | MS.gene92024:intron | 35.0% |
| AACATGGCCGAAGTAGAATT+AGG | - | chr7.3:79991440-79991459 | None:intergenic | 40.0% | |
| AGGGATATGATTCAGCATTG+TGG | - | chr7.3:79990486-79990505 | None:intergenic | 40.0% | |
| CAAGGGTAAGCTATAACAGT+TGG | + | chr7.3:79991146-79991165 | MS.gene92024:CDS | 40.0% | |
| CTGCTTGAACTAAGATAAGG+AGG | - | chr7.3:79991785-79991804 | None:intergenic | 40.0% | |
| GGACTGCTTGAACTAAGATA+AGG | - | chr7.3:79991788-79991807 | None:intergenic | 40.0% | |
| GGGATATGATTCAGCATTGT+GGG | - | chr7.3:79990485-79990504 | None:intergenic | 40.0% | |
| ! | TCACCTGGATACCCTTTTAA+AGG | + | chr7.3:79990682-79990701 | MS.gene92024:CDS | 40.0% |
| ! | TGATGGATGGAGTTGAAGTA+GGG | - | chr7.3:79991814-79991833 | None:intergenic | 40.0% |
| !! | ATGATGGATGGAGTTGAAGT+AGG | - | chr7.3:79991815-79991834 | None:intergenic | 40.0% |
| !! | GTACCTTTAAAAGGGTATCC+AGG | - | chr7.3:79990688-79990707 | None:intergenic | 40.0% |
| AGAGGGAATGACAAGAGATG+TGG | + | chr7.3:79991745-79991764 | MS.gene92024:CDS | 45.0% | |
| AGGGAATGACAAGAGATGTG+GGG | + | chr7.3:79991747-79991766 | MS.gene92024:CDS | 45.0% | |
| ATGCAATCCACTTTCAGGAG+AGG | + | chr7.3:79991231-79991250 | MS.gene92024:CDS | 45.0% | |
| ATTGGTACCTCTCCTGAAAG+TGG | - | chr7.3:79991241-79991260 | None:intergenic | 45.0% | |
| CAACTCCATCCATCATAGAG+AGG | + | chr7.3:79991819-79991838 | MS.gene92024:CDS | 45.0% | |
| GAGGGAATGACAAGAGATGT+GGG | + | chr7.3:79991746-79991765 | MS.gene92024:CDS | 45.0% | |
| TCTAGTACTGCTTCTTCACC+TGG | + | chr7.3:79990667-79990686 | MS.gene92024:CDS | 45.0% | |
| TCTTTCACCACTGCCCTTAA+AGG | + | chr7.3:79990825-79990844 | MS.gene92024:CDS | 45.0% | |
| ! | AAGCTATTCTGGAGAGCTAC+CGG | - | chr7.3:79991579-79991598 | None:intergenic | 45.0% |
| !! | ACTTTCAGGAGAGGTACCAA+TGG | + | chr7.3:79991240-79991259 | MS.gene92024:CDS | 45.0% |
| !! | GGGAGTGTTTATCTCCAGAA+GGG | + | chr7.3:79991167-79991186 | MS.gene92024:CDS | 45.0% |
| !! | TGGGAGTGTTTATCTCCAGA+AGG | + | chr7.3:79991166-79991185 | MS.gene92024:CDS | 45.0% |
| !!! | TAATTTTTTTTTTTAGTAAA+AGG | + | chr7.3:79990854-79990873 | MS.gene92024:intron | 5.0% |
| !!! | TTTCTTATATTTTATATTTT+TGG | + | chr7.3:79991074-79991093 | MS.gene92024:intron | 5.0% |
| GATGGAGTTGAAGTAGGGCT+AGG | - | chr7.3:79991809-79991828 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 79990478 | 79992072 | 79990478 | ID=MS.gene92024 |
| chr7.3 | mRNA | 79990478 | 79992072 | 79990478 | ID=MS.gene92024.t1;Parent=MS.gene92024 |
| chr7.3 | exon | 79990478 | 79990703 | 79990478 | ID=MS.gene92024.t1.exon1;Parent=MS.gene92024.t1 |
| chr7.3 | CDS | 79990478 | 79990703 | 79990478 | ID=cds.MS.gene92024.t1;Parent=MS.gene92024.t1 |
| chr7.3 | exon | 79990802 | 79990846 | 79990802 | ID=MS.gene92024.t1.exon2;Parent=MS.gene92024.t1 |
| chr7.3 | CDS | 79990802 | 79990846 | 79990802 | ID=cds.MS.gene92024.t1;Parent=MS.gene92024.t1 |
| chr7.3 | exon | 79991137 | 79991418 | 79991137 | ID=MS.gene92024.t1.exon3;Parent=MS.gene92024.t1 |
| chr7.3 | CDS | 79991137 | 79991418 | 79991137 | ID=cds.MS.gene92024.t1;Parent=MS.gene92024.t1 |
| chr7.3 | exon | 79991735 | 79991907 | 79991735 | ID=MS.gene92024.t1.exon4;Parent=MS.gene92024.t1 |
| chr7.3 | CDS | 79991735 | 79991907 | 79991735 | ID=cds.MS.gene92024.t1;Parent=MS.gene92024.t1 |
| chr7.3 | exon | 79992034 | 79992072 | 79992034 | ID=MS.gene92024.t1.exon5;Parent=MS.gene92024.t1 |
| chr7.3 | CDS | 79992034 | 79992072 | 79992034 | ID=cds.MS.gene92024.t1;Parent=MS.gene92024.t1 |
| Gene Sequence |
| Protein sequence |
