Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92195.t1 | AFK43929.1 | 98.6 | 70 | 1 | 0 | 1 | 70 | 1 | 70 | 1.40E-29 | 138.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92195.t1 | Q9ASY8 | 63.1 | 65 | 22 | 1 | 8 | 70 | 11 | 75 | 1.8e-17 | 89.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92195.t1 | I3T329 | 98.6 | 70 | 1 | 0 | 1 | 70 | 1 | 70 | 1.0e-29 | 138.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene06001 | MS.gene92195 | 0.80933 | 2.00E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92195.t1 | MTR_2g067370 | 98.571 | 70 | 1 | 0 | 1 | 70 | 1 | 70 | 2.79e-45 | 139 |
| MS.gene92195.t1 | MTR_3g462060 | 90.000 | 70 | 7 | 0 | 1 | 70 | 1 | 70 | 8.24e-41 | 128 |
| MS.gene92195.t1 | MTR_3g055500 | 85.507 | 69 | 10 | 0 | 1 | 69 | 1 | 69 | 1.31e-38 | 124 |
| MS.gene92195.t1 | MTR_3g061970 | 80.000 | 70 | 14 | 0 | 1 | 70 | 1 | 70 | 6.78e-35 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92195.t1 | AT5G41685 | 67.308 | 52 | 17 | 0 | 19 | 70 | 24 | 75 | 2.18e-23 | 84.3 |
| MS.gene92195.t1 | AT1G64220 | 70.455 | 44 | 13 | 0 | 27 | 70 | 34 | 77 | 9.29e-19 | 72.8 |
Find 18 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTGAGAAATTTGTGGTTT+TGG | 0.210299 | 2.4:-27219617 | None:intergenic |
| ACAAAGGTTATTGCTCACTA+TGG | 0.348329 | 2.4:+27219554 | MS.gene92195:CDS |
| ATTCCTTTGATTATCATCAT+TGG | 0.388372 | 2.4:+27219581 | MS.gene92195:CDS |
| CTGAGAAGCTGAGAAATTTG+TGG | 0.449278 | 2.4:-27219623 | None:intergenic |
| GAAAAGAAGAATGGCGCCTA+GGG | 0.517130 | 2.4:+27219433 | None:intergenic |
| CTGTGAAGGAATGGACAACA+TGG | 0.524007 | 2.4:+27219519 | MS.gene92195:CDS |
| TGATCGATTCTGTGAAGGAA+TGG | 0.539433 | 2.4:+27219510 | MS.gene92195:CDS |
| TTTGCTTTCAGAGAAACCCT+AGG | 0.540311 | 2.4:-27219449 | None:intergenic |
| AGGGTTTCTCTGAAAGCAAA+GGG | 0.547703 | 2.4:+27219452 | MS.gene92195:CDS |
| AGAAAAGAAGAATGGCGCCT+AGG | 0.558230 | 2.4:+27219432 | None:intergenic |
| TAGGGTTTCTCTGAAAGCAA+AGG | 0.590299 | 2.4:+27219451 | MS.gene92195:CDS |
| ATACCAATGATGATAATCAA+AGG | 0.596226 | 2.4:-27219584 | None:intergenic |
| ATCAGTGATCGATTCTGTGA+AGG | 0.598645 | 2.4:+27219505 | MS.gene92195:CDS |
| TGTGAAGGAATGGACAACAT+GGG | 0.649737 | 2.4:+27219520 | MS.gene92195:CDS |
| ATGGGGTATGAAGAAAACAA+AGG | 0.679010 | 2.4:+27219538 | MS.gene92195:CDS |
| GTGAAGGAATGGACAACATG+GGG | 0.691902 | 2.4:+27219521 | MS.gene92195:CDS |
| ATGTGCACATCGATTACACA+GGG | 0.703734 | 2.4:-27219646 | None:intergenic |
| AATGTGCACATCGATTACAC+AGG | 0.707397 | 2.4:-27219647 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATACCAATGATGATAATCAA+AGG | - | chr2.4:27219587-27219606 | None:intergenic | 25.0% |
| ! | ATTCCTTTGATTATCATCAT+TGG | + | chr2.4:27219581-27219600 | MS.gene92195:CDS | 25.0% |
| ACAAAGGTTATTGCTCACTA+TGG | + | chr2.4:27219554-27219573 | MS.gene92195:CDS | 35.0% | |
| ATGGGGTATGAAGAAAACAA+AGG | + | chr2.4:27219538-27219557 | MS.gene92195:CDS | 35.0% | |
| ! | AGCTGAGAAATTTGTGGTTT+TGG | - | chr2.4:27219620-27219639 | None:intergenic | 35.0% |
| CTGAGAAGCTGAGAAATTTG+TGG | - | chr2.4:27219626-27219645 | None:intergenic | 40.0% | |
| TGTGAAGGAATGGACAACAT+GGG | + | chr2.4:27219520-27219539 | MS.gene92195:CDS | 40.0% | |
| TTTGCTTTCAGAGAAACCCT+AGG | - | chr2.4:27219452-27219471 | None:intergenic | 40.0% | |
| ! | AGGGTTTCTCTGAAAGCAAA+GGG | + | chr2.4:27219452-27219471 | MS.gene92195:CDS | 40.0% |
| ! | ATCAGTGATCGATTCTGTGA+AGG | + | chr2.4:27219505-27219524 | MS.gene92195:CDS | 40.0% |
| ! | TAGGGTTTCTCTGAAAGCAA+AGG | + | chr2.4:27219451-27219470 | MS.gene92195:CDS | 40.0% |
| ! | TGATCGATTCTGTGAAGGAA+TGG | + | chr2.4:27219510-27219529 | MS.gene92195:CDS | 40.0% |
| CTGTGAAGGAATGGACAACA+TGG | + | chr2.4:27219519-27219538 | MS.gene92195:CDS | 45.0% | |
| GTGAAGGAATGGACAACATG+GGG | + | chr2.4:27219521-27219540 | MS.gene92195:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 27219443 | 27219655 | 27219443 | ID=MS.gene92195 |
| chr2.4 | mRNA | 27219443 | 27219655 | 27219443 | ID=MS.gene92195.t1;Parent=MS.gene92195 |
| chr2.4 | exon | 27219443 | 27219655 | 27219443 | ID=MS.gene92195.t1.exon1;Parent=MS.gene92195.t1 |
| chr2.4 | CDS | 27219443 | 27219655 | 27219443 | ID=cds.MS.gene92195.t1;Parent=MS.gene92195.t1 |
| Gene Sequence |
| Protein sequence |
