Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92223.t1 | AES77205.1 | 59.5 | 200 | 20 | 3 | 1 | 158 | 1 | 181 | 6.80E-43 | 183.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92223.t1 | P52855 | 68.3 | 63 | 20 | 0 | 1 | 63 | 1 | 63 | 2.2e-18 | 93.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92223.t1 | G7KWM5 | 59.5 | 200 | 20 | 3 | 1 | 158 | 1 | 181 | 4.9e-43 | 183.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057576 | MS.gene92223 | 0.84503 | 5.24E-59 | -1.69E-46 |
| MS.gene057584 | MS.gene92223 | 0.840869 | 6.70E-58 | -1.69E-46 |
| MS.gene057586 | MS.gene92223 | 0.806681 | 7.31E-50 | -1.69E-46 |
| MS.gene057588 | MS.gene92223 | 0.82091 | 5.34E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92223.t1 | MTR_7g005740 | 61.000 | 200 | 17 | 3 | 1 | 158 | 1 | 181 | 1.06e-59 | 184 |
| MS.gene92223.t1 | MTR_3g092600 | 95.082 | 61 | 3 | 0 | 1 | 61 | 1 | 61 | 9.19e-37 | 123 |
| MS.gene92223.t1 | MTR_2g066750 | 95.082 | 61 | 3 | 0 | 1 | 61 | 1 | 61 | 9.19e-37 | 123 |
| MS.gene92223.t1 | MTR_8g024350 | 66.176 | 68 | 23 | 0 | 1 | 68 | 1 | 68 | 7.00e-27 | 97.8 |
| MS.gene92223.t1 | MTR_7g118060 | 66.102 | 59 | 20 | 0 | 1 | 59 | 1 | 59 | 1.19e-19 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92223.t1 | AT5G24510 | 52.727 | 110 | 31 | 2 | 1 | 89 | 1 | 110 | 1.18e-27 | 100 |
| MS.gene92223.t1 | AT1G01100 | 51.064 | 94 | 38 | 3 | 2 | 90 | 3 | 93 | 4.61e-23 | 87.8 |
| MS.gene92223.t1 | AT5G47700 | 63.333 | 60 | 22 | 0 | 2 | 61 | 3 | 62 | 4.87e-23 | 88.2 |
| MS.gene92223.t1 | AT5G47700 | 63.333 | 60 | 22 | 0 | 2 | 61 | 3 | 62 | 4.87e-23 | 88.2 |
| MS.gene92223.t1 | AT1G01100 | 61.667 | 60 | 23 | 0 | 2 | 61 | 3 | 62 | 1.83e-22 | 86.7 |
| MS.gene92223.t1 | AT1G01100 | 61.667 | 60 | 23 | 0 | 2 | 61 | 3 | 62 | 1.83e-22 | 86.7 |
| MS.gene92223.t1 | AT1G01100 | 61.667 | 60 | 23 | 0 | 2 | 61 | 3 | 62 | 1.83e-22 | 86.7 |
| MS.gene92223.t1 | AT4G00810 | 60.000 | 60 | 24 | 0 | 2 | 61 | 3 | 62 | 1.23e-21 | 84.7 |
| MS.gene92223.t1 | AT4G00810 | 60.000 | 60 | 24 | 0 | 2 | 61 | 3 | 62 | 1.23e-21 | 84.7 |
Find 29 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGTCGTGCAAAGTATATTT+TGG | 0.240282 | 7.1:-87098119 | None:intergenic |
| ACATAATATAATCAACTTCT+AGG | 0.271094 | 7.1:-87098271 | None:intergenic |
| GATAATAGACTTACGGTGAT+TGG | 0.316212 | 7.1:-87097304 | None:intergenic |
| GATGATGATATAGGTTGTTT+TGG | 0.338031 | 7.1:+87098059 | MS.gene92223:CDS |
| TCATCTGAGCTCTCGACTTC+AGG | 0.351887 | 7.1:-87098041 | None:intergenic |
| TCTGAGCAAGCTTAGCGAAT+AGG | 0.386873 | 7.1:-87097450 | None:intergenic |
| ATCTTTGATAGTCGTACTAA+TGG | 0.401580 | 7.1:+87098140 | MS.gene92223:CDS |
| GATGAGAATGGCAGAAAATT+CGG | 0.405059 | 7.1:+87098242 | MS.gene92223:CDS |
| AAAATGAGTTCCGGCGAGTT+GGG | 0.423502 | 7.1:+87097241 | None:intergenic |
| GCAAGCTTAGCGAATAGGTT+TGG | 0.436712 | 7.1:-87097445 | None:intergenic |
| ATAATAGACTTACGGTGATT+GGG | 0.445449 | 7.1:-87097303 | None:intergenic |
| GAAGATCGGCACTTTGTTGA+AGG | 0.446681 | 7.1:+87097393 | MS.gene92223:CDS |
| TTTGATGACTTTGATTGCAT+TGG | 0.451838 | 7.1:+87098197 | MS.gene92223:CDS |
| GATTATATTATGTGCGTTAG+TGG | 0.457016 | 7.1:+87098281 | MS.gene92223:CDS |
| TTGATGACTTTGATTGCATT+GGG | 0.462464 | 7.1:+87098198 | MS.gene92223:CDS |
| TTGAACTCCGGCTCTGTTGG+TGG | 0.474989 | 7.1:+87097496 | MS.gene92223:CDS |
| GAGAGCTCAGATGATGATAT+AGG | 0.478337 | 7.1:+87098050 | MS.gene92223:CDS |
| ATTATATTATGTGCGTTAGT+GGG | 0.495034 | 7.1:+87098282 | MS.gene92223:CDS |
| GGCGGCACCACCAACAGAGC+CGG | 0.512281 | 7.1:-87097503 | None:intergenic |
| AACTCAGACGTTGATGAGAA+TGG | 0.522044 | 7.1:+87098230 | MS.gene92223:CDS |
| ACGTCACCGTTGAATCCTAC+TGG | 0.524586 | 7.1:+87097422 | MS.gene92223:CDS |
| ACCTTGATCCTCCACGACGA+TGG | 0.533754 | 7.1:+87097277 | MS.gene92223:CDS |
| TCCATCGTCGTGGAGGATCA+AGG | 0.539738 | 7.1:-87097278 | None:intergenic |
| GGCTGCGGGACAAGTGAGGA+AGG | 0.571317 | 7.1:+87098080 | MS.gene92223:CDS |
| AGCGAATAGGTTTGGCCAGT+AGG | 0.571609 | 7.1:-87097437 | None:intergenic |
| GTTTGGCCAGTAGGATTCAA+CGG | 0.591956 | 7.1:-87097428 | None:intergenic |
| GTAAATGCAACCCAACTCGC+CGG | 0.634662 | 7.1:-87097251 | None:intergenic |
| TTGGGATTCCATCGTCGTGG+AGG | 0.650788 | 7.1:-87097285 | None:intergenic |
| TGATTGGGATTCCATCGTCG+TGG | 0.662981 | 7.1:-87097288 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTAAAATAAAAAGTACAAT+TGG | - | chr7.1:87097847-87097866 | None:intergenic | 10.0% |
| !!! | AGATCTCTTTTTTTTTTTTT+AGG | + | chr7.1:87097366-87097385 | MS.gene92223:intron | 15.0% |
| !! | ACATAATATAATCAACTTCT+AGG | - | chr7.1:87098274-87098293 | None:intergenic | 20.0% |
| !! | GAAAAAAGATAATAGACTTA+CGG | - | chr7.1:87097314-87097333 | None:intergenic | 20.0% |
| !! | GAATAATCAAAACAATAATC+AGG | + | chr7.1:87097689-87097708 | MS.gene92223:intron | 20.0% |
| !!! | AATTGGTTTATAAAATGAGA+AGG | - | chr7.1:87097830-87097849 | None:intergenic | 20.0% |
| !!! | ATTGGTTTATAAAATGAGAA+GGG | - | chr7.1:87097829-87097848 | None:intergenic | 20.0% |
| !!! | TCTCTTTTTTTTTTTTTAGG+CGG | + | chr7.1:87097369-87097388 | MS.gene92223:intron | 20.0% |
| ! | ATTAAAATATACTCACCCTT+GGG | + | chr7.1:87097957-87097976 | MS.gene92223:intron | 25.0% |
| ! | ATTATATTATGTGCGTTAGT+GGG | + | chr7.1:87098282-87098301 | MS.gene92223:CDS | 25.0% |
| ! | TGAAAAAGAAATAACCGAAA+AGG | - | chr7.1:87097587-87097606 | None:intergenic | 25.0% |
| ! | TGGAATTATTCAAAACTTGT+TGG | + | chr7.1:87097642-87097661 | MS.gene92223:intron | 25.0% |
| ! | TTGGTTTATAAAATGAGAAG+GGG | - | chr7.1:87097828-87097847 | None:intergenic | 25.0% |
| !! | GGAATTATTCAAAACTTGTT+GGG | + | chr7.1:87097643-87097662 | MS.gene92223:intron | 25.0% |
| AAAGAGATCTATTAGCGATT+TGG | - | chr7.1:87097356-87097375 | None:intergenic | 30.0% | |
| ATAATAGACTTACGGTGATT+GGG | - | chr7.1:87097306-87097325 | None:intergenic | 30.0% | |
| GATTAAAATATACTCACCCT+TGG | + | chr7.1:87097956-87097975 | MS.gene92223:intron | 30.0% | |
| GATTATATTATGTGCGTTAG+TGG | + | chr7.1:87098281-87098300 | MS.gene92223:CDS | 30.0% | |
| TCAAAGCATACATATGATCA+GGG | + | chr7.1:87097750-87097769 | MS.gene92223:intron | 30.0% | |
| TGATCAATTAAGAAACCCAA+GGG | - | chr7.1:87097975-87097994 | None:intergenic | 30.0% | |
| TGGTTTATAAAATGAGAAGG+GGG | - | chr7.1:87097827-87097846 | None:intergenic | 30.0% | |
| TTGATCAATTAAGAAACCCA+AGG | - | chr7.1:87097976-87097995 | None:intergenic | 30.0% | |
| ! | ATCTTTGATAGTCGTACTAA+TGG | + | chr7.1:87098140-87098159 | MS.gene92223:CDS | 30.0% |
| ! | ATGTCGTGCAAAGTATATTT+TGG | - | chr7.1:87098122-87098141 | None:intergenic | 30.0% |
| ! | GATGATGATATAGGTTGTTT+TGG | + | chr7.1:87098059-87098078 | MS.gene92223:CDS | 30.0% |
| ! | TTGATGACTTTGATTGCATT+GGG | + | chr7.1:87098198-87098217 | MS.gene92223:CDS | 30.0% |
| ! | TTTGATGACTTTGATTGCAT+TGG | + | chr7.1:87098197-87098216 | MS.gene92223:CDS | 30.0% |
| !!! | AGCAACTTCTTTTTTGTCTT+CGG | - | chr7.1:87098018-87098037 | None:intergenic | 30.0% |
| CTCAAAGCATACATATGATC+AGG | + | chr7.1:87097749-87097768 | MS.gene92223:intron | 35.0% | |
| GATAATAGACTTACGGTGAT+TGG | - | chr7.1:87097307-87097326 | None:intergenic | 35.0% | |
| GATGAGAATGGCAGAAAATT+CGG | + | chr7.1:87098242-87098261 | MS.gene92223:CDS | 35.0% | |
| GCTTGTGAGATATTCAAAGA+CGG | - | chr7.1:87097780-87097799 | None:intergenic | 35.0% | |
| TGATTCTCTTCTGTATTGCT+TGG | + | chr7.1:87097622-87097641 | MS.gene92223:intron | 35.0% | |
| ! | GATGATCTCATTTTGAACTC+CGG | + | chr7.1:87097484-87097503 | MS.gene92223:CDS | 35.0% |
| !!! | GCAACTTCTTTTTTGTCTTC+GGG | - | chr7.1:87098017-87098036 | None:intergenic | 35.0% |
| !!! | TTTTTTTAGGCGGAGAAGAT+CGG | + | chr7.1:87097379-87097398 | MS.gene92223:intron | 35.0% |
| GAAATAACCGAAAAGGAACG+AGG | - | chr7.1:87097580-87097599 | None:intergenic | 40.0% | |
| GAGAGCTCAGATGATGATAT+AGG | + | chr7.1:87098050-87098069 | MS.gene92223:CDS | 40.0% | |
| ! | AACTCAGACGTTGATGAGAA+TGG | + | chr7.1:87098230-87098249 | MS.gene92223:CDS | 40.0% |
| !!! | ATATAGGTTGTTTTGGCTGC+GGG | + | chr7.1:87098066-87098085 | MS.gene92223:CDS | 40.0% |
| !!! | GATATAGGTTGTTTTGGCTG+CGG | + | chr7.1:87098065-87098084 | MS.gene92223:CDS | 40.0% |
| AGAAGTTGCTAAGCAGAGAG+AGG | + | chr7.1:87097722-87097741 | MS.gene92223:intron | 45.0% | |
| AGCATGAGCAAGCTTAGCAA+CGG | - | chr7.1:87097527-87097546 | None:intergenic | 45.0% | |
| GAAGATCGGCACTTTGTTGA+AGG | + | chr7.1:87097393-87097412 | MS.gene92223:CDS | 45.0% | |
| GCAAGCTTAGCGAATAGGTT+TGG | - | chr7.1:87097448-87097467 | None:intergenic | 45.0% | |
| GTTTGGCCAGTAGGATTCAA+CGG | - | chr7.1:87097431-87097450 | None:intergenic | 45.0% | |
| TCTGAGCAAGCTTAGCGAAT+AGG | - | chr7.1:87097453-87097472 | None:intergenic | 45.0% | |
| ! | ATCTCACAAGCGTCGCAAAT+GGG | + | chr7.1:87097789-87097808 | MS.gene92223:intron | 45.0% |
| ! | ATTTTGAACTCCGGCTCTGT+TGG | + | chr7.1:87097493-87097512 | MS.gene92223:CDS | 45.0% |
| ! | GTCTTCTCCTCGTTCCTTTT+CGG | + | chr7.1:87097570-87097589 | MS.gene92223:intron | 45.0% |
| ! | TATCTCACAAGCGTCGCAAA+TGG | + | chr7.1:87097788-87097807 | MS.gene92223:intron | 45.0% |
| ACGTCACCGTTGAATCCTAC+TGG | + | chr7.1:87097422-87097441 | MS.gene92223:CDS | 50.0% | |
| AGCGAATAGGTTTGGCCAGT+AGG | - | chr7.1:87097440-87097459 | None:intergenic | 50.0% | |
| ATGAGCAAGCTTAGCAACGG+CGG | - | chr7.1:87097524-87097543 | None:intergenic | 50.0% | |
| GTAAATGCAACCCAACTCGC+CGG | - | chr7.1:87097254-87097273 | None:intergenic | 50.0% | |
| TCATCTGAGCTCTCGACTTC+AGG | - | chr7.1:87098044-87098063 | None:intergenic | 50.0% | |
| TGATTGGGATTCCATCGTCG+TGG | - | chr7.1:87097291-87097310 | None:intergenic | 50.0% | |
| ACCTTGATCCTCCACGACGA+TGG | + | chr7.1:87097277-87097296 | MS.gene92223:CDS | 55.0% | |
| TCCATCGTCGTGGAGGATCA+AGG | - | chr7.1:87097281-87097300 | None:intergenic | 55.0% | |
| TTGAACTCCGGCTCTGTTGG+TGG | + | chr7.1:87097496-87097515 | MS.gene92223:CDS | 55.0% | |
| TTGGGATTCCATCGTCGTGG+AGG | - | chr7.1:87097288-87097307 | None:intergenic | 55.0% | |
| !! | TTTTGGCTGCGGGACAAGTG+AGG | + | chr7.1:87098076-87098095 | MS.gene92223:CDS | 55.0% |
| GGCTGCGGGACAAGTGAGGA+AGG | + | chr7.1:87098080-87098099 | MS.gene92223:CDS | 65.0% | |
| GGCGGCACCACCAACAGAGC+CGG | - | chr7.1:87097506-87097525 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 87097244 | 87098310 | 87097244 | ID=MS.gene92223 |
| chr7.1 | mRNA | 87097244 | 87098310 | 87097244 | ID=MS.gene92223.t1;Parent=MS.gene92223 |
| chr7.1 | exon | 87097244 | 87097312 | 87097244 | ID=MS.gene92223.t1.exon1;Parent=MS.gene92223.t1 |
| chr7.1 | CDS | 87097244 | 87097312 | 87097244 | ID=cds.MS.gene92223.t1;Parent=MS.gene92223.t1 |
| chr7.1 | exon | 87097388 | 87097517 | 87097388 | ID=MS.gene92223.t1.exon2;Parent=MS.gene92223.t1 |
| chr7.1 | CDS | 87097388 | 87097517 | 87097388 | ID=cds.MS.gene92223.t1;Parent=MS.gene92223.t1 |
| chr7.1 | exon | 87098012 | 87098310 | 87098012 | ID=MS.gene92223.t1.exon3;Parent=MS.gene92223.t1 |
| chr7.1 | CDS | 87098012 | 87098310 | 87098012 | ID=cds.MS.gene92223.t1;Parent=MS.gene92223.t1 |
| Gene Sequence |
| Protein sequence |
