Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92323.t1 | XP_003610667.1 | 93.7 | 159 | 10 | 0 | 1 | 159 | 38 | 196 | 6.30E-80 | 307 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92323.t1 | Q84MC0 | 62.2 | 156 | 56 | 2 | 2 | 157 | 39 | 191 | 9.9e-50 | 198.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92323.t1 | B7FGN4 | 93.7 | 159 | 10 | 0 | 1 | 159 | 38 | 196 | 4.5e-80 | 307.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050297 | MS.gene92323 | 0.802353 | 5.84E-49 | -1.69E-46 |
| MS.gene052143 | MS.gene92323 | 0.826373 | 2.81E-54 | -1.69E-46 |
| MS.gene056526 | MS.gene92323 | 0.803983 | 2.69E-49 | -1.69E-46 |
| MS.gene06057 | MS.gene92323 | 0.809742 | 1.63E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92323.t1 | MTR_5g005710 | 93.711 | 159 | 10 | 0 | 1 | 159 | 38 | 196 | 1.78e-107 | 306 |
| MS.gene92323.t1 | MTR_1g105305 | 68.553 | 159 | 44 | 1 | 2 | 160 | 43 | 195 | 1.95e-77 | 230 |
| MS.gene92323.t1 | MTR_1g082770 | 40.625 | 128 | 74 | 2 | 2 | 127 | 44 | 171 | 6.19e-30 | 109 |
| MS.gene92323.t1 | MTR_7g113640 | 40.741 | 135 | 77 | 2 | 2 | 133 | 40 | 174 | 1.07e-29 | 108 |
| MS.gene92323.t1 | MTR_1g082830 | 37.500 | 128 | 78 | 2 | 2 | 127 | 59 | 186 | 1.43e-25 | 98.6 |
| MS.gene92323.t1 | MTR_1g082790 | 35.577 | 104 | 66 | 1 | 1 | 103 | 57 | 160 | 5.87e-18 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92323.t1 | AT3G06035 | 62.179 | 156 | 56 | 2 | 2 | 157 | 39 | 191 | 2.52e-64 | 197 |
| MS.gene92323.t1 | AT5G19250 | 65.909 | 132 | 45 | 0 | 2 | 133 | 40 | 171 | 2.01e-60 | 187 |
| MS.gene92323.t1 | AT1G54860 | 42.138 | 159 | 88 | 4 | 2 | 156 | 42 | 200 | 1.33e-41 | 139 |
| MS.gene92323.t1 | AT5G19240 | 47.170 | 159 | 70 | 4 | 2 | 157 | 40 | 187 | 1.48e-40 | 136 |
| MS.gene92323.t1 | AT5G19230 | 52.381 | 126 | 51 | 2 | 2 | 127 | 40 | 156 | 3.57e-36 | 125 |
| MS.gene92323.t1 | AT5G19230 | 52.381 | 126 | 51 | 2 | 2 | 127 | 40 | 156 | 3.57e-36 | 125 |
Find 46 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAGCTTTATCACCAAAAT+TGG | 0.148873 | 5.1:+781879 | MS.gene92323:CDS |
| GACTCAAAGTTTACTGGAAT+TGG | 0.202771 | 5.1:+781753 | MS.gene92323:CDS |
| GTAATGCCTGCTTGTGTTCC+TGG | 0.222223 | 5.1:+781669 | MS.gene92323:CDS |
| GGATTGGTTCCGAAGATAAT+TGG | 0.229248 | 5.1:+781775 | MS.gene92323:CDS |
| CTTAATGACTCAAAGTTTAC+TGG | 0.244932 | 5.1:+781747 | MS.gene92323:CDS |
| TTAAGTGACATTTAGTTAAA+AGG | 0.252789 | 5.1:-781620 | None:intergenic |
| GCAGTAAACCAATCCAATTT+TGG | 0.309912 | 5.1:-781892 | None:intergenic |
| TACTATCACTGTCTGGGAAA+TGG | 0.319924 | 5.1:+782031 | MS.gene92323:CDS |
| TTGTGTTCCTGGCCTTGTTC+CGG | 0.361906 | 5.1:+781680 | MS.gene92323:CDS |
| CAGTGAAGCATAGAGGGATA+TGG | 0.369607 | 5.1:+782085 | MS.gene92323:CDS |
| AGTGATAGTATAAGCCTATA+TGG | 0.384109 | 5.1:-782018 | None:intergenic |
| ACTATCACTGTCTGGGAAAT+GGG | 0.388974 | 5.1:+782032 | MS.gene92323:CDS |
| ACTCAAAGTTTACTGGAATT+GGG | 0.389579 | 5.1:+781754 | MS.gene92323:CDS |
| TACAAGGTTGATTCTTGAAC+TGG | 0.391876 | 5.1:-781536 | None:intergenic |
| AGGCTTATACTATCACTGTC+TGG | 0.401288 | 5.1:+782024 | MS.gene92323:CDS |
| GTTAAAAGGTCTTCATAGTC+GGG | 0.420487 | 5.1:-781606 | None:intergenic |
| CTTTATCACCAAAATTGGAT+TGG | 0.444400 | 5.1:+781884 | MS.gene92323:CDS |
| AACAACAATCCAATTATCTT+CGG | 0.461927 | 5.1:-781784 | None:intergenic |
| CTCGTCATCACTAGAACTCT+CGG | 0.472274 | 5.1:-781844 | None:intergenic |
| AGTTAAAAGGTCTTCATAGT+CGG | 0.473244 | 5.1:-781607 | None:intergenic |
| AGTGATGACGAGACTTCTAA+TGG | 0.482206 | 5.1:+781855 | MS.gene92323:CDS |
| AAGTTTACTGGAATTGGGAT+TGG | 0.486609 | 5.1:+781759 | MS.gene92323:CDS |
| ACAATGTCCGGAACAAGGCC+AGG | 0.505794 | 5.1:-781687 | None:intergenic |
| TACAGATCCATCCCTTGTGT+TGG | 0.505945 | 5.1:-781649 | None:intergenic |
| TTGCAGCATAGTCGGTAAGC+AGG | 0.507546 | 5.1:+781987 | MS.gene92323:intron |
| GCACCTGTGGTATTTGTACA+AGG | 0.509795 | 5.1:-781552 | None:intergenic |
| AATATTTCCAACACAAGGGA+TGG | 0.513314 | 5.1:+781642 | MS.gene92323:CDS |
| ACAAGGCCAGGAACACAAGC+AGG | 0.520245 | 5.1:-781675 | None:intergenic |
| GGCTTATACTATCACTGTCT+GGG | 0.523682 | 5.1:+782025 | MS.gene92323:CDS |
| TATAAGCCTATATGGTAGCT+TGG | 0.531909 | 5.1:-782010 | None:intergenic |
| AAATTAGAGAGGACAATGTC+CGG | 0.542734 | 5.1:-781699 | None:intergenic |
| GGTAATTGAGGCTCAGTACC+TGG | 0.543020 | 5.1:-781585 | None:intergenic |
| AGCAGGCCAAGCTACCATAT+AGG | 0.557003 | 5.1:+782004 | MS.gene92323:CDS |
| TCTTCATAGTCGGGTAATTG+AGG | 0.557889 | 5.1:-781597 | None:intergenic |
| ACTTAAATATTTCCAACACA+AGG | 0.568858 | 5.1:+781637 | MS.gene92323:CDS |
| TGGTATTGTGTTAGCACCTG+TGG | 0.587893 | 5.1:-781565 | None:intergenic |
| TGGGAAACTGCAAATAGTGA+TGG | 0.590466 | 5.1:+782051 | MS.gene92323:CDS |
| TCGTCATCACTAGAACTCTC+GGG | 0.593940 | 5.1:-781843 | None:intergenic |
| GAGATTTCGTGAAATTAGAG+AGG | 0.595369 | 5.1:-781710 | None:intergenic |
| CAACCTTGTACAAATACCAC+AGG | 0.614385 | 5.1:+781549 | MS.gene92323:CDS |
| AGAGGACAATGTCCGGAACA+AGG | 0.617052 | 5.1:-781692 | None:intergenic |
| ACAGGTGCTAACACAATACC+AGG | 0.623473 | 5.1:+781567 | MS.gene92323:CDS |
| CTTAAATATTTCCAACACAA+GGG | 0.640170 | 5.1:+781638 | MS.gene92323:CDS |
| GCTTCCGTCAGAAGTGCTTG+TGG | 0.642715 | 5.1:-781814 | None:intergenic |
| TTGACCACAAGCACTTCTGA+CGG | 0.644420 | 5.1:+781810 | MS.gene92323:CDS |
| ATGTCAAGTTCAATGATGTG+CGG | 0.702075 | 5.1:-781464 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAGTGACATTTAGTTAAA+AGG | - | chr5.1:781623-781642 | None:intergenic | 20.0% |
| ! | AACAACAATCCAATTATCTT+CGG | - | chr5.1:781787-781806 | None:intergenic | 25.0% |
| ! | ACTTAAATATTTCCAACACA+AGG | + | chr5.1:781637-781656 | MS.gene92323:CDS | 25.0% |
| ! | CTTAAATATTTCCAACACAA+GGG | + | chr5.1:781638-781657 | MS.gene92323:CDS | 25.0% |
| ACTCAAAGTTTACTGGAATT+GGG | + | chr5.1:781754-781773 | MS.gene92323:CDS | 30.0% | |
| AGTGATAGTATAAGCCTATA+TGG | - | chr5.1:782021-782040 | None:intergenic | 30.0% | |
| AGTTAAAAGGTCTTCATAGT+CGG | - | chr5.1:781610-781629 | None:intergenic | 30.0% | |
| CTTAATGACTCAAAGTTTAC+TGG | + | chr5.1:781747-781766 | MS.gene92323:CDS | 30.0% | |
| CTTTATCACCAAAATTGGAT+TGG | + | chr5.1:781884-781903 | MS.gene92323:CDS | 30.0% | |
| ! | CATTTTTATTGCAGCATAGT+CGG | + | chr5.1:781979-781998 | MS.gene92323:intron | 30.0% |
| ! | GAAAGCTTTATCACCAAAAT+TGG | + | chr5.1:781879-781898 | MS.gene92323:CDS | 30.0% |
| AAATTAGAGAGGACAATGTC+CGG | - | chr5.1:781702-781721 | None:intergenic | 35.0% | |
| AAGTTTACTGGAATTGGGAT+TGG | + | chr5.1:781759-781778 | MS.gene92323:CDS | 35.0% | |
| AATATTTCCAACACAAGGGA+TGG | + | chr5.1:781642-781661 | MS.gene92323:CDS | 35.0% | |
| ATGTCAAGTTCAATGATGTG+CGG | - | chr5.1:781467-781486 | None:intergenic | 35.0% | |
| GACTCAAAGTTTACTGGAAT+TGG | + | chr5.1:781753-781772 | MS.gene92323:CDS | 35.0% | |
| GAGATTTCGTGAAATTAGAG+AGG | - | chr5.1:781713-781732 | None:intergenic | 35.0% | |
| GTTAAAAGGTCTTCATAGTC+GGG | - | chr5.1:781609-781628 | None:intergenic | 35.0% | |
| TATAAGCCTATATGGTAGCT+TGG | - | chr5.1:782013-782032 | None:intergenic | 35.0% | |
| ! | GCAGTAAACCAATCCAATTT+TGG | - | chr5.1:781895-781914 | None:intergenic | 35.0% |
| ! | TACAAGGTTGATTCTTGAAC+TGG | - | chr5.1:781539-781558 | None:intergenic | 35.0% |
| !! | GTTTTTCAGTGAAGCATAGA+GGG | + | chr5.1:782079-782098 | MS.gene92323:CDS | 35.0% |
| !! | TGTTTTTCAGTGAAGCATAG+AGG | + | chr5.1:782078-782097 | MS.gene92323:CDS | 35.0% |
| ACTATCACTGTCTGGGAAAT+GGG | + | chr5.1:782032-782051 | MS.gene92323:CDS | 40.0% | |
| AGGCTTATACTATCACTGTC+TGG | + | chr5.1:782024-782043 | MS.gene92323:CDS | 40.0% | |
| CAACCTTGTACAAATACCAC+AGG | + | chr5.1:781549-781568 | MS.gene92323:CDS | 40.0% | |
| GGCTTATACTATCACTGTCT+GGG | + | chr5.1:782025-782044 | MS.gene92323:CDS | 40.0% | |
| TACTATCACTGTCTGGGAAA+TGG | + | chr5.1:782031-782050 | MS.gene92323:CDS | 40.0% | |
| TCTTCATAGTCGGGTAATTG+AGG | - | chr5.1:781600-781619 | None:intergenic | 40.0% | |
| TGCTCATTGCTCTTGTTAGT+TGG | + | chr5.1:781912-781931 | MS.gene92323:intron | 40.0% | |
| TGGGAAACTGCAAATAGTGA+TGG | + | chr5.1:782051-782070 | MS.gene92323:CDS | 40.0% | |
| ! | AGTGATGACGAGACTTCTAA+TGG | + | chr5.1:781855-781874 | MS.gene92323:CDS | 40.0% |
| !! | ATTATCCTGTTTTGAGCGTG+AGG | + | chr5.1:781939-781958 | MS.gene92323:intron | 40.0% |
| !! | GGATTGGTTCCGAAGATAAT+TGG | + | chr5.1:781775-781794 | MS.gene92323:CDS | 40.0% |
| ACAGGTGCTAACACAATACC+AGG | + | chr5.1:781567-781586 | MS.gene92323:CDS | 45.0% | |
| CAGTGAAGCATAGAGGGATA+TGG | + | chr5.1:782085-782104 | MS.gene92323:CDS | 45.0% | |
| CTCGTCATCACTAGAACTCT+CGG | - | chr5.1:781847-781866 | None:intergenic | 45.0% | |
| GCACCTGTGGTATTTGTACA+AGG | - | chr5.1:781555-781574 | None:intergenic | 45.0% | |
| TACAGATCCATCCCTTGTGT+TGG | - | chr5.1:781652-781671 | None:intergenic | 45.0% | |
| TCGTCATCACTAGAACTCTC+GGG | - | chr5.1:781846-781865 | None:intergenic | 45.0% | |
| TGGTATTGTGTTAGCACCTG+TGG | - | chr5.1:781568-781587 | None:intergenic | 45.0% | |
| TTGACCACAAGCACTTCTGA+CGG | + | chr5.1:781810-781829 | MS.gene92323:CDS | 45.0% | |
| AGAGGACAATGTCCGGAACA+AGG | - | chr5.1:781695-781714 | None:intergenic | 50.0% | |
| AGCAGGCCAAGCTACCATAT+AGG | + | chr5.1:782004-782023 | MS.gene92323:CDS | 50.0% | |
| AGCATCCTCACGCTCAAAAC+AGG | - | chr5.1:781947-781966 | None:intergenic | 50.0% | |
| GGTAATTGAGGCTCAGTACC+TGG | - | chr5.1:781588-781607 | None:intergenic | 50.0% | |
| GTAATGCCTGCTTGTGTTCC+TGG | + | chr5.1:781669-781688 | MS.gene92323:CDS | 50.0% | |
| TTGCAGCATAGTCGGTAAGC+AGG | + | chr5.1:781987-782006 | MS.gene92323:intron | 50.0% | |
| !! | TTGTGTTCCTGGCCTTGTTC+CGG | + | chr5.1:781680-781699 | MS.gene92323:CDS | 50.0% |
| ACAAGGCCAGGAACACAAGC+AGG | - | chr5.1:781678-781697 | None:intergenic | 55.0% | |
| ACAATGTCCGGAACAAGGCC+AGG | - | chr5.1:781690-781709 | None:intergenic | 55.0% | |
| !! | GCTTCCGTCAGAAGTGCTTG+TGG | - | chr5.1:781817-781836 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 781459 | 782110 | 781459 | ID=MS.gene92323 |
| chr5.1 | mRNA | 781459 | 782110 | 781459 | ID=MS.gene92323.t1;Parent=MS.gene92323 |
| chr5.1 | exon | 781459 | 781925 | 781459 | ID=MS.gene92323.t1.exon1;Parent=MS.gene92323.t1 |
| chr5.1 | CDS | 781459 | 781925 | 781459 | ID=cds.MS.gene92323.t1;Parent=MS.gene92323.t1 |
| chr5.1 | exon | 781993 | 782110 | 781993 | ID=MS.gene92323.t1.exon2;Parent=MS.gene92323.t1 |
| chr5.1 | CDS | 781993 | 782110 | 781993 | ID=cds.MS.gene92323.t1;Parent=MS.gene92323.t1 |
| Gene Sequence |
| Protein sequence |
