Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92337.t1 | XP_018459315.1 | 46.6 | 339 | 158 | 6 | 1 | 338 | 1 | 317 | 3.70E-67 | 265.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92337.t1 | A0A2N9I863 | 41.6 | 356 | 170 | 7 | 1 | 339 | 1 | 335 | 4.4e-62 | 248.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052447 | MS.gene92337 | 0.824369 | 8.38E-54 | -1.69E-46 |
| MS.gene05415 | MS.gene92337 | 0.821905 | 3.15E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92337.t1 | MTR_7g062720 | 40.541 | 148 | 80 | 3 | 1 | 146 | 1 | 142 | 6.56e-32 | 117 |
| MS.gene92337.t1 | MTR_4g037355 | 38.686 | 137 | 62 | 3 | 155 | 290 | 1 | 116 | 1.48e-21 | 89.7 |
| MS.gene92337.t1 | MTR_5g061150 | 33.333 | 111 | 74 | 0 | 40 | 150 | 26 | 136 | 1.17e-17 | 79.0 |
| MS.gene92337.t1 | MTR_4g112730 | 57.971 | 69 | 28 | 1 | 143 | 211 | 2 | 69 | 1.37e-17 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 59 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTATTATTGCCCTCATTAA+TGG | 0.192737 | 5.1:-534252 | MS.gene92337:CDS |
| TGGATCAATGGAACAAATTA+AGG | 0.196151 | 5.1:-534232 | MS.gene92337:CDS |
| AAAGTTGTGGCGTTTATTTC+AGG | 0.203868 | 5.1:+533936 | None:intergenic |
| CAAAAGATCCCAAGATCTTA+TGG | 0.237847 | 5.1:-534310 | MS.gene92337:CDS |
| GACAAAATTAAAGCAAGATT+TGG | 0.252197 | 5.1:-534287 | MS.gene92337:CDS |
| GATTCTTGAGGGAGACGATT+TGG | 0.271296 | 5.1:-534429 | MS.gene92337:CDS |
| CACGACCTCTACCACGAAAA+TGG | 0.272842 | 5.1:+533820 | None:intergenic |
| CTCAATTAAGTTGTTGTTCC+AGG | 0.274617 | 5.1:+534478 | None:intergenic |
| ATTTACTGAATACTCTGATT+TGG | 0.325598 | 5.1:-534042 | MS.gene92337:CDS |
| AGACATGTAGTGTCATTCTT+TGG | 0.340063 | 5.1:+533564 | None:intergenic |
| TATTGAAGGAGAATTTAATA+TGG | 0.344429 | 5.1:-533526 | MS.gene92337:CDS |
| ATTCTTGAGGGAGACGATTT+GGG | 0.354692 | 5.1:-534428 | MS.gene92337:CDS |
| GATTTCTAGAGGGACCTTCC+TGG | 0.373636 | 5.1:+533721 | None:intergenic |
| CATATCCACCACGTCCTCTT+TGG | 0.374344 | 5.1:+533886 | None:intergenic |
| ACAAAATTAAAGCAAGATTT+GGG | 0.407053 | 5.1:-534286 | MS.gene92337:CDS |
| CGTGGTGGATATGCTCGTTT+TGG | 0.409902 | 5.1:-533876 | MS.gene92337:CDS |
| TGCCCTCATTAATGGATCAA+TGG | 0.432677 | 5.1:-534244 | MS.gene92337:CDS |
| ACAATCAAACAAGAATCATC+AGG | 0.437067 | 5.1:-533760 | MS.gene92337:CDS |
| TATAACTGAAGTGGAAAAGT+TGG | 0.441180 | 5.1:-534129 | MS.gene92337:CDS |
| AAAAGATCCCAAGATCTTAT+GGG | 0.458748 | 5.1:-534309 | MS.gene92337:CDS |
| CGTGGTAGAGGTCGTGGAAG+AGG | 0.462783 | 5.1:-533813 | MS.gene92337:CDS |
| AACCATCAGCTGCGTCCTAC+AGG | 0.465355 | 5.1:-533972 | MS.gene92337:CDS |
| AACAAGAATCATCAGGGAAA+AGG | 0.472157 | 5.1:-533753 | MS.gene92337:CDS |
| ATCTGTGGATGGAAAGGAAA+AGG | 0.477668 | 5.1:-533610 | MS.gene92337:CDS |
| ATGTGGAAAGAAAGGTCATT+GGG | 0.480192 | 5.1:-533679 | MS.gene92337:CDS |
| GATGTGGAAAGAAAGGTCAT+TGG | 0.491426 | 5.1:-533680 | MS.gene92337:CDS |
| ATCAAGCACCACATTGATGA+TGG | 0.492315 | 5.1:-534356 | MS.gene92337:CDS |
| TGTTATAGATGTGGAAAGAA+AGG | 0.499593 | 5.1:-533687 | MS.gene92337:CDS |
| CTGGCGACAACTACATAACC+TGG | 0.502097 | 5.1:-534496 | MS.gene92337:CDS |
| GTTCCATTGATCCATTAATG+AGG | 0.509644 | 5.1:+534241 | None:intergenic |
| GGACTAGACAAGATTCTTGA+GGG | 0.521095 | 5.1:-534440 | MS.gene92337:CDS |
| TGCAACAACAATATAGAATG+AGG | 0.523271 | 5.1:-534067 | MS.gene92337:CDS |
| CAAAGAATCTGTGGATGGAA+AGG | 0.532926 | 5.1:-533616 | MS.gene92337:CDS |
| TTCCATTGATCCATTAATGA+GGG | 0.533283 | 5.1:+534242 | None:intergenic |
| TTCCTGTAGGACGCAGCTGA+TGG | 0.535010 | 5.1:+533970 | None:intergenic |
| TGGCAAAACTATAACTGAAG+TGG | 0.552382 | 5.1:-534138 | MS.gene92337:CDS |
| CGTGGTCGAGGCAGAAGTCA+TGG | 0.557420 | 5.1:-533849 | MS.gene92337:CDS |
| TTTGATTGTTCATATTTGAG+AGG | 0.564015 | 5.1:+533774 | None:intergenic |
| GTCATTGGGCTAAAACATGC+AGG | 0.564978 | 5.1:-533665 | MS.gene92337:CDS |
| AATTGAGTATCTTGCATGTG+AGG | 0.565013 | 5.1:-534462 | MS.gene92337:CDS |
| ATTGAGTATCTTGCATGTGA+GGG | 0.575966 | 5.1:-534461 | MS.gene92337:CDS |
| GGATATGCCATTGTTCCTGT+AGG | 0.578642 | 5.1:+533957 | None:intergenic |
| TTTGGTTGCAGCTCTTCTAG+TGG | 0.579996 | 5.1:-534024 | MS.gene92337:CDS |
| TAAAAGATACAAAGAATCTG+TGG | 0.594054 | 5.1:-533625 | MS.gene92337:CDS |
| AGATACAAAGAATCTGTGGA+TGG | 0.602731 | 5.1:-533621 | MS.gene92337:CDS |
| GCAACAACAATATAGAATGA+GGG | 0.604762 | 5.1:-534066 | MS.gene92337:CDS |
| ACGCCCTTGATTAAAAGTTG+TGG | 0.608515 | 5.1:+533923 | None:intergenic |
| TATAATCGCCAAAGAGGACG+TGG | 0.608957 | 5.1:-533894 | MS.gene92337:CDS |
| ACTATCATCATGATTTCTAG+AGG | 0.612820 | 5.1:+533710 | None:intergenic |
| GGGACTAGACAAGATTCTTG+AGG | 0.613207 | 5.1:-534441 | MS.gene92337:CDS |
| GATGATAGTTGTTATAGATG+TGG | 0.615697 | 5.1:-533696 | MS.gene92337:CDS |
| CAATCAAACAAGAATCATCA+GGG | 0.626443 | 5.1:-533759 | MS.gene92337:CDS |
| GAATGACACTACATGTCTTG+AGG | 0.641228 | 5.1:-533559 | MS.gene92337:CDS |
| GGTGGTTATAATCGCCAAAG+AGG | 0.642086 | 5.1:-533900 | MS.gene92337:CDS |
| GCTGCGTCCTACAGGAACAA+TGG | 0.649882 | 5.1:-533964 | MS.gene92337:CDS |
| CTATCATCATGATTTCTAGA+GGG | 0.659075 | 5.1:+533711 | None:intergenic |
| AATCGCCAAAGAGGACGTGG+TGG | 0.675947 | 5.1:-533891 | MS.gene92337:CDS |
| TTTGTAATCCATCATCAATG+TGG | 0.715751 | 5.1:+534348 | None:intergenic |
| CGATTTGGGAGATGTCCCAG+AGG | 0.725435 | 5.1:-534414 | MS.gene92337:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATTAAAGCAAGATTT+GGG | - | chr5.1:533771-533790 | MS.gene92337:CDS | 20.0% |
| !! | TATTGAAGGAGAATTTAATA+TGG | - | chr5.1:534531-534550 | MS.gene92337:CDS | 20.0% |
| !!! | TTCAAAATTTTAAGCATAAC+TGG | - | chr5.1:533542-533561 | MS.gene92337:CDS | 20.0% |
| ! | ATTTACTGAATACTCTGATT+TGG | - | chr5.1:534015-534034 | MS.gene92337:CDS | 25.0% |
| ! | GACAAAATTAAAGCAAGATT+TGG | - | chr5.1:533770-533789 | MS.gene92337:CDS | 25.0% |
| ! | TAAAAGATACAAAGAATCTG+TGG | - | chr5.1:534432-534451 | MS.gene92337:CDS | 25.0% |
| ! | TTTGATTGTTCATATTTGAG+AGG | + | chr5.1:534286-534305 | None:intergenic | 25.0% |
| !! | ATTGCACAATTAGAATTTTG+TGG | - | chr5.1:533899-533918 | MS.gene92337:CDS | 25.0% |
| !! | TATCTTTTACATAGATACTC+TGG | + | chr5.1:534421-534440 | None:intergenic | 25.0% |
| !!! | CAACAACAGTTTTATAATCT+TGG | + | chr5.1:533853-533872 | None:intergenic | 25.0% |
| AAAAGATCCCAAGATCTTAT+GGG | - | chr5.1:533748-533767 | MS.gene92337:CDS | 30.0% | |
| ACAATCAAACAAGAATCATC+AGG | - | chr5.1:534297-534316 | MS.gene92337:CDS | 30.0% | |
| AGTATTATTGCCCTCATTAA+TGG | - | chr5.1:533805-533824 | MS.gene92337:CDS | 30.0% | |
| CAATCAAACAAGAATCATCA+GGG | - | chr5.1:534298-534317 | MS.gene92337:CDS | 30.0% | |
| GATGATAGTTGTTATAGATG+TGG | - | chr5.1:534361-534380 | MS.gene92337:CDS | 30.0% | |
| GCAACAACAATATAGAATGA+GGG | - | chr5.1:533991-534010 | MS.gene92337:CDS | 30.0% | |
| TATAACTGAAGTGGAAAAGT+TGG | - | chr5.1:533928-533947 | MS.gene92337:CDS | 30.0% | |
| TGCAACAACAATATAGAATG+AGG | - | chr5.1:533990-534009 | MS.gene92337:CDS | 30.0% | |
| TGGATCAATGGAACAAATTA+AGG | - | chr5.1:533825-533844 | MS.gene92337:CDS | 30.0% | |
| TGTTATAGATGTGGAAAGAA+AGG | - | chr5.1:534370-534389 | MS.gene92337:CDS | 30.0% | |
| TTCCATTGATCCATTAATGA+GGG | + | chr5.1:533818-533837 | None:intergenic | 30.0% | |
| TTTGTAATCCATCATCAATG+TGG | + | chr5.1:533712-533731 | None:intergenic | 30.0% | |
| ! | AATTTTGTCCCATAAGATCT+TGG | + | chr5.1:533759-533778 | None:intergenic | 30.0% |
| ! | ACTATCATCATGATTTCTAG+AGG | + | chr5.1:534350-534369 | None:intergenic | 30.0% |
| ! | ATTTTGTCCCATAAGATCTT+GGG | + | chr5.1:533758-533777 | None:intergenic | 30.0% |
| ! | CTATCATCATGATTTCTAGA+GGG | + | chr5.1:534349-534368 | None:intergenic | 30.0% |
| ! | TTTTCTTTGCTAATTCCTCT+GGG | + | chr5.1:533661-533680 | None:intergenic | 30.0% |
| ! | TTTTTCTTTGCTAATTCCTC+TGG | + | chr5.1:533662-533681 | None:intergenic | 30.0% |
| AAAGGTAAAAACATCCAGGA+AGG | - | chr5.1:534322-534341 | MS.gene92337:CDS | 35.0% | |
| AAAGTTGTGGCGTTTATTTC+AGG | + | chr5.1:534124-534143 | None:intergenic | 35.0% | |
| AACAAGAATCATCAGGGAAA+AGG | - | chr5.1:534304-534323 | MS.gene92337:CDS | 35.0% | |
| AATTGAGTATCTTGCATGTG+AGG | - | chr5.1:533595-533614 | MS.gene92337:CDS | 35.0% | |
| AGATACAAAGAATCTGTGGA+TGG | - | chr5.1:534436-534455 | MS.gene92337:CDS | 35.0% | |
| ATGTGGAAAGAAAGGTCATT+GGG | - | chr5.1:534378-534397 | MS.gene92337:CDS | 35.0% | |
| ATTGAGTATCTTGCATGTGA+GGG | - | chr5.1:533596-533615 | MS.gene92337:CDS | 35.0% | |
| CAAAAGATCCCAAGATCTTA+TGG | - | chr5.1:533747-533766 | MS.gene92337:CDS | 35.0% | |
| GTTCCATTGATCCATTAATG+AGG | + | chr5.1:533819-533838 | None:intergenic | 35.0% | |
| TGGCAAAACTATAACTGAAG+TGG | - | chr5.1:533919-533938 | MS.gene92337:CDS | 35.0% | |
| ! | AACGCCACAACTTTTAATCA+AGG | - | chr5.1:534130-534149 | MS.gene92337:CDS | 35.0% |
| ! | ACGCCACAACTTTTAATCAA+GGG | - | chr5.1:534131-534150 | MS.gene92337:CDS | 35.0% |
| ! | AGACATGTAGTGTCATTCTT+TGG | + | chr5.1:534496-534515 | None:intergenic | 35.0% |
| ! | CTCAATTAAGTTGTTGTTCC+AGG | + | chr5.1:533582-533601 | None:intergenic | 35.0% |
| ACGCCCTTGATTAAAAGTTG+TGG | + | chr5.1:534137-534156 | None:intergenic | 40.0% | |
| ATCAAGCACCACATTGATGA+TGG | - | chr5.1:533701-533720 | MS.gene92337:CDS | 40.0% | |
| ATCTGTGGATGGAAAGGAAA+AGG | - | chr5.1:534447-534466 | MS.gene92337:CDS | 40.0% | |
| ATTCTTGAGGGAGACGATTT+GGG | - | chr5.1:533629-533648 | MS.gene92337:CDS | 40.0% | |
| CAAAGAATCTGTGGATGGAA+AGG | - | chr5.1:534441-534460 | MS.gene92337:CDS | 40.0% | |
| GAATGACACTACATGTCTTG+AGG | - | chr5.1:534498-534517 | MS.gene92337:CDS | 40.0% | |
| GATGTGGAAAGAAAGGTCAT+TGG | - | chr5.1:534377-534396 | MS.gene92337:CDS | 40.0% | |
| GGACTAGACAAGATTCTTGA+GGG | - | chr5.1:533617-533636 | MS.gene92337:CDS | 40.0% | |
| GGGAAAAGGTAAAAACATCC+AGG | - | chr5.1:534318-534337 | MS.gene92337:CDS | 40.0% | |
| ! | AACTTTTCATGCATCTCAGG+TGG | - | chr5.1:533964-533983 | MS.gene92337:CDS | 40.0% |
| ! | ACAACTTTTAATCAAGGGCG+TGG | - | chr5.1:534136-534155 | MS.gene92337:CDS | 40.0% |
| ! | AGAAACCATTTTCGTGGTAG+AGG | - | chr5.1:534232-534251 | MS.gene92337:CDS | 40.0% |
| ! | CATGGTAGAAACCATTTTCG+TGG | - | chr5.1:534226-534245 | MS.gene92337:CDS | 40.0% |
| ! | CTCAACTTTTCATGCATCTC+AGG | - | chr5.1:533961-533980 | MS.gene92337:CDS | 40.0% |
| ! | TGCCCTCATTAATGGATCAA+TGG | - | chr5.1:533813-533832 | MS.gene92337:CDS | 40.0% |
| !!! | GAGGCTGCTGATTTTATTGA+AGG | - | chr5.1:534517-534536 | MS.gene92337:CDS | 40.0% |
| GATTCTTGAGGGAGACGATT+TGG | - | chr5.1:533628-533647 | MS.gene92337:CDS | 45.0% | |
| GGATATGCCATTGTTCCTGT+AGG | + | chr5.1:534103-534122 | None:intergenic | 45.0% | |
| GGGACTAGACAAGATTCTTG+AGG | - | chr5.1:533616-533635 | MS.gene92337:CDS | 45.0% | |
| GGTGGTTATAATCGCCAAAG+AGG | - | chr5.1:534157-534176 | MS.gene92337:CDS | 45.0% | |
| GTCATTGGGCTAAAACATGC+AGG | - | chr5.1:534392-534411 | MS.gene92337:CDS | 45.0% | |
| TATAATCGCCAAAGAGGACG+TGG | - | chr5.1:534163-534182 | MS.gene92337:CDS | 45.0% | |
| TTTGGTTGCAGCTCTTCTAG+TGG | - | chr5.1:534033-534052 | MS.gene92337:CDS | 45.0% | |
| !! | ACTTTTAATCAAGGGCGTGG+TGG | - | chr5.1:534139-534158 | MS.gene92337:CDS | 45.0% |
| CACGACCTCTACCACGAAAA+TGG | + | chr5.1:534240-534259 | None:intergenic | 50.0% | |
| CATATCCACCACGTCCTCTT+TGG | + | chr5.1:534174-534193 | None:intergenic | 50.0% | |
| CTGGCGACAACTACATAACC+TGG | - | chr5.1:533561-533580 | MS.gene92337:CDS | 50.0% | |
| ! | CATTTTCGTGGTAGAGGTCG+TGG | - | chr5.1:534238-534257 | MS.gene92337:CDS | 50.0% |
| ! | GATTTCTAGAGGGACCTTCC+TGG | + | chr5.1:534339-534358 | None:intergenic | 50.0% |
| ! | GGTGGATATGCTCGTTTTGG+TGG | - | chr5.1:534184-534203 | MS.gene92337:CDS | 50.0% |
| !! | TATGCTCGTTTTGGTGGTCG+TGG | - | chr5.1:534190-534209 | MS.gene92337:CDS | 50.0% |
| !!! | CGTGGTGGATATGCTCGTTT+TGG | - | chr5.1:534181-534200 | MS.gene92337:CDS | 50.0% |
| AACCATCAGCTGCGTCCTAC+AGG | - | chr5.1:534085-534104 | MS.gene92337:CDS | 55.0% | |
| AATCGCCAAAGAGGACGTGG+TGG | - | chr5.1:534166-534185 | MS.gene92337:CDS | 55.0% | |
| GCTGCGTCCTACAGGAACAA+TGG | - | chr5.1:534093-534112 | MS.gene92337:CDS | 55.0% | |
| TTCCTGTAGGACGCAGCTGA+TGG | + | chr5.1:534090-534109 | None:intergenic | 55.0% | |
| ! | CGATTTGGGAGATGTCCCAG+AGG | - | chr5.1:533643-533662 | MS.gene92337:CDS | 55.0% |
| CGTGGTAGAGGTCGTGGAAG+AGG | - | chr5.1:534244-534263 | MS.gene92337:CDS | 60.0% | |
| CGTGGTCGAGGCAGAAGTCA+TGG | - | chr5.1:534208-534227 | MS.gene92337:CDS | 60.0% | |
| !! | CGTTTTGGTGGTCGTGGTCG+AGG | - | chr5.1:534196-534215 | MS.gene92337:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 533521 | 534558 | 533521 | ID=MS.gene92337 |
| chr5.1 | mRNA | 533521 | 534558 | 533521 | ID=MS.gene92337.t1;Parent=MS.gene92337 |
| chr5.1 | exon | 533521 | 534558 | 533521 | ID=MS.gene92337.t1.exon1;Parent=MS.gene92337.t1 |
| chr5.1 | CDS | 533521 | 534558 | 533521 | ID=cds.MS.gene92337.t1;Parent=MS.gene92337.t1 |
| Gene Sequence |
| Protein sequence |
