AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92410.t1 MTR_8g038250 88.610 439 26 2 1 438 1 416 0.0 774
MS.gene92410.t1 MTR_8g096990 78.311 438 89 2 1 438 1 432 0.0 702
MS.gene92410.t1 MTR_5g080890 73.469 441 107 6 1 438 1 434 0.0 645
MS.gene92410.t1 MTR_8g038280 71.719 442 115 3 1 438 1 436 0.0 644
MS.gene92410.t1 MTR_5g080960 73.243 441 108 6 1 438 1 434 0.0 642
MS.gene92410.t1 MTR_5g044770 69.635 438 126 3 1 438 1 431 0.0 612
MS.gene92410.t1 MTR_6g090290 64.706 442 145 4 1 437 1 436 0.0 553
MS.gene92410.t1 MTR_8g038310 60.731 438 146 5 1 437 1 413 0.0 526
MS.gene92410.t1 MTR_4g109760 64.920 439 113 7 1 438 1 399 0.0 513
MS.gene92410.t1 MTR_3g028120 53.571 420 185 6 25 438 24 439 6.63e-150 433
MS.gene92410.t1 MTR_5g038360 50.797 439 202 7 5 437 6 436 6.23e-142 413
MS.gene92410.t1 MTR_2g067830 52.670 412 183 7 32 437 1 406 8.15e-138 401
MS.gene92410.t1 MTR_6g088760 52.709 406 176 8 1 395 1 401 7.06e-136 400
MS.gene92410.t1 MTR_5g044570 48.315 445 188 10 1 437 1 411 3.94e-131 384
MS.gene92410.t1 MTR_0024s0040 50.239 418 190 9 30 437 1 410 3.57e-126 372
MS.gene92410.t1 MTR_2g105300 49.034 414 167 8 32 434 1 381 2.37e-123 363
MS.gene92410.t1 MTR_0036s0050 43.946 446 221 11 7 437 4 435 9.00e-114 341
MS.gene92410.t1 MTR_0036s0070 41.950 441 228 11 7 437 4 426 2.48e-109 329
MS.gene92410.t1 MTR_0036s0110 41.099 455 237 13 1 437 1 442 2.53e-100 306
MS.gene92410.t1 MTR_5g008070 42.180 422 219 10 21 436 23 425 7.93e-99 302
MS.gene92410.t1 MTR_2g059490 39.908 436 224 9 8 438 9 411 2.48e-92 286
MS.gene92410.t1 MTR_2g059460 40.284 422 205 10 23 437 25 406 6.97e-92 284
MS.gene92410.t1 MTR_5g008150 40.047 422 211 11 22 437 27 412 2.21e-90 280
MS.gene92410.t1 MTR_5g008110 38.673 437 223 11 6 437 19 415 1.03e-89 278
MS.gene92410.t1 MTR_3g097150 35.371 458 256 17 4 438 9 449 3.90e-76 244
MS.gene92410.t1 MTR_1g060750 37.531 405 216 12 40 437 2 376 2.48e-73 235
MS.gene92410.t1 MTR_4g045857 38.870 301 151 5 16 312 17 288 3.48e-60 198
MS.gene92410.t1 MTR_5g008090 37.748 302 174 8 142 437 21 314 6.36e-58 193
MS.gene92410.t1 MTR_7g117430 34.642 433 239 18 25 435 74 484 3.66e-54 188
MS.gene92410.t1 MTR_1g115210 32.258 434 255 15 21 435 73 486 1.72e-53 186
MS.gene92410.t1 MTR_1g039270 34.341 364 205 16 85 435 127 469 2.02e-49 175
MS.gene92410.t1 MTR_3g046300 32.041 387 243 9 68 438 79 461 1.28e-47 170
MS.gene92410.t1 MTR_6g085010 34.648 355 208 13 86 435 156 491 2.63e-46 167
MS.gene92410.t1 MTR_1g111350 33.983 359 208 12 85 435 130 467 1.09e-45 164
MS.gene92410.t1 MTR_6g088765 40.336 238 108 5 163 399 5 209 5.34e-44 154
MS.gene92410.t1 MTR_5g091130 31.795 390 241 11 68 438 173 556 1.16e-42 158
MS.gene92410.t1 MTR_2g028190 32.603 365 218 12 85 435 101 451 3.29e-40 149
MS.gene92410.t1 MTR_4g007740 29.877 405 247 14 49 436 94 478 1.52e-39 148
MS.gene92410.t1 MTR_3g053710 32.507 363 221 12 85 435 116 466 6.25e-39 146
MS.gene92410.t1 MTR_2g070430 28.508 449 266 17 28 437 25 457 5.45e-36 138
MS.gene92410.t1 MTR_8g103880 29.609 358 226 13 87 436 117 456 1.35e-35 137
MS.gene92410.t1 MTR_2g055840 29.581 382 232 12 78 435 5 373 2.03e-35 135
MS.gene92410.t1 MTR_7g080230 27.477 444 262 17 30 436 11 431 4.02e-34 133
MS.gene92410.t1 MTR_7g105850 29.193 483 268 22 8 437 6 467 5.20e-33 130
MS.gene92410.t1 MTR_1g078240 28.395 405 241 15 62 436 45 430 3.09e-32 128
MS.gene92410.t1 MTR_1g078240 28.818 406 239 16 62 436 45 431 1.22e-31 126
MS.gene92410.t1 MTR_3g068000 28.982 383 219 15 89 435 76 441 1.63e-30 123
MS.gene92410.t1 MTR_7g021510 29.381 388 221 19 82 436 67 434 2.88e-30 122
MS.gene92410.t1 MTR_3g065160 26.683 401 247 13 67 438 47 429 3.39e-30 121
MS.gene92410.t1 MTR_4g008110 55.752 113 27 1 162 274 48 137 1.97e-29 112
MS.gene92410.t1 MTR_8g094590 27.204 397 244 14 65 438 53 427 1.52e-27 114
MS.gene92410.t1 MTR_3g053980 31.680 363 218 13 85 435 141 485 4.15e-27 113
MS.gene92410.t1 MTR_4g095270 25.359 418 264 17 41 438 37 426 5.54e-27 114
MS.gene92410.t1 MTR_4g095270 25.359 418 264 17 41 438 37 426 7.79e-27 113
MS.gene92410.t1 MTR_3g096930 25.515 388 242 13 78 438 70 437 3.20e-26 110
MS.gene92410.t1 MTR_5g016260 25.061 411 250 16 49 438 61 434 3.60e-26 111
MS.gene92410.t1 MTR_5g077850 25.984 381 229 14 88 438 83 440 4.03e-26 110
MS.gene92410.t1 MTR_7g062360 27.865 384 226 16 89 438 62 428 4.18e-26 110
MS.gene92410.t1 MTR_5g016260 25.061 411 250 16 49 438 61 434 4.84e-26 111
MS.gene92410.t1 MTR_4g056420 29.630 378 224 15 85 436 76 437 7.62e-26 109
MS.gene92410.t1 MTR_2g063110 24.895 478 275 19 28 435 45 508 8.22e-26 110
MS.gene92410.t1 MTR_1g029810 26.330 376 236 14 85 438 56 412 1.56e-25 108
MS.gene92410.t1 MTR_1g029810 26.064 376 237 13 85 438 56 412 2.53e-25 107
MS.gene92410.t1 MTR_7g080230 28.045 353 209 15 110 436 28 361 6.38e-25 106
MS.gene92410.t1 MTR_4g023630 26.683 401 246 15 66 438 42 422 7.09e-25 106
MS.gene92410.t1 MTR_1g037630 28.467 411 262 16 25 423 26 416 2.15e-24 105
MS.gene92410.t1 MTR_4g023650 26.368 402 246 16 66 438 42 422 5.91e-24 103
MS.gene92410.t1 MTR_1g078240 28.614 339 196 13 62 373 45 364 6.92e-24 102
MS.gene92410.t1 MTR_6g084710 25.891 421 257 18 49 435 151 550 2.07e-23 103
MS.gene92410.t1 MTR_8g094590 31.047 277 164 10 65 334 53 309 2.22e-23 100
MS.gene92410.t1 MTR_8g039540 28.506 435 275 17 7 423 5 421 2.74e-23 102
MS.gene92410.t1 MTR_2g103340 36.813 182 65 4 20 199 33 166 5.00e-23 96.3
MS.gene92410.t1 MTR_2g103340 46.341 82 34 3 351 432 88 159 8.99e-12 64.3
MS.gene92410.t1 MTR_1g061940 29.243 383 223 18 84 436 81 445 5.85e-23 101
MS.gene92410.t1 MTR_3g014290 30.904 343 212 13 87 423 81 404 7.32e-23 100
MS.gene92410.t1 MTR_8g094590 30.070 286 173 10 65 343 53 318 1.10e-22 99.8
MS.gene92410.t1 MTR_0945s0010 38.760 129 74 4 311 438 1 125 1.92e-22 94.0
MS.gene92410.t1 MTR_2g084550 29.474 380 223 16 85 436 76 438 3.61e-22 99.0
MS.gene92410.t1 MTR_7g102750 28.165 387 218 20 85 436 80 441 3.74e-21 96.3
MS.gene92410.t1 MTR_6g084710 27.628 333 204 16 49 365 151 462 6.94e-21 95.5
MS.gene92410.t1 MTR_4g100990 27.338 417 222 21 86 438 74 473 2.72e-20 93.6
MS.gene92410.t1 MTR_5g061690 29.296 355 198 20 93 423 108 433 5.67e-19 89.7
MS.gene92410.t1 MTR_8g075090 27.441 379 217 19 87 437 113 461 8.86e-18 85.9
MS.gene92410.t1 MTR_7g102750 28.923 325 181 16 85 379 80 384 9.86e-18 85.5
MS.gene92410.t1 MTR_5g077920 27.005 374 234 13 88 437 70 428 1.36e-17 85.1
MS.gene92410.t1 MTR_8g075090 27.368 380 218 19 87 438 113 462 1.42e-17 85.1
MS.gene92410.t1 MTR_8g075090 29.605 304 172 14 92 374 118 400 1.06e-16 82.4
MS.gene92410.t1 MTR_8g075010 25.654 382 224 18 87 438 101 452 5.52e-15 77.4
MS.gene92410.t1 MTR_2g037710 27.650 217 124 7 228 436 15 206 4.00e-14 71.6
MS.gene92410.t1 MTR_3g100500 27.913 412 253 20 30 422 38 424 2.22e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92410.t1 AT1G64830 51.208 414 195 3 26 438 24 431 2.04e-139 406
MS.gene92410.t1 AT5G33340 52.771 415 186 5 27 438 30 437 8.48e-137 400
MS.gene92410.t1 AT2G35615 45.740 446 219 8 8 436 5 444 7.02e-118 352
MS.gene92410.t1 AT1G31450 43.750 448 223 9 4 436 9 442 2.19e-107 325
MS.gene92410.t1 AT2G28010 40.181 443 211 12 1 438 1 394 7.89e-92 283
MS.gene92410.t1 AT2G28040 39.955 443 211 13 1 438 1 393 5.32e-89 276
MS.gene92410.t1 AT2G28040 41.587 416 190 12 26 438 19 384 3.05e-87 271
MS.gene92410.t1 AT2G28030 44.022 368 176 11 75 438 49 390 2.19e-85 266
MS.gene92410.t1 AT2G28220 37.892 446 223 12 1 438 1 400 1.79e-84 265
MS.gene92410.t1 AT2G28225 37.136 447 225 12 1 438 1 400 3.44e-79 251
MS.gene92410.t1 AT2G03200 36.384 437 238 14 25 438 42 461 9.15e-70 228
MS.gene92410.t1 AT1G01300 38.017 363 197 14 85 436 140 485 7.86e-54 187
MS.gene92410.t1 AT3G61820 36.616 396 216 15 55 435 108 483 3.55e-53 185
MS.gene92410.t1 AT3G18490 31.776 428 264 15 18 435 91 500 8.91e-53 184
MS.gene92410.t1 AT4G30030 36.243 378 212 15 72 438 63 422 1.28e-52 182
MS.gene92410.t1 AT2G23945 27.609 460 287 14 8 438 9 451 2.10e-48 172
MS.gene92410.t1 AT5G10760 32.634 429 251 14 23 435 58 464 1.48e-46 167
MS.gene92410.t1 AT3G25700 31.019 432 244 17 39 436 39 450 2.76e-46 166
MS.gene92410.t1 AT1G25510 33.973 365 200 15 85 435 146 483 6.67e-46 166
MS.gene92410.t1 AT3G20015 30.248 443 243 18 28 435 59 470 1.55e-44 162
MS.gene92410.t1 AT5G36260 31.926 379 211 15 85 436 76 434 2.26e-44 161
MS.gene92410.t1 AT5G10770 32.719 434 246 17 29 436 61 474 7.57e-42 154
MS.gene92410.t1 AT4G30040 30.464 453 251 21 8 430 9 427 1.20e-40 150
MS.gene92410.t1 AT2G42980 32.992 391 230 14 72 438 145 527 6.34e-40 150
MS.gene92410.t1 AT3G02740 30.667 375 220 14 85 436 83 440 9.23e-40 149
MS.gene92410.t1 AT3G02740 30.027 373 221 13 87 436 10 365 9.72e-40 147
MS.gene92410.t1 AT3G59080 32.051 390 233 15 72 438 155 535 1.59e-39 149
MS.gene92410.t1 AT1G65240 29.454 421 243 16 49 436 28 427 1.56e-38 145
MS.gene92410.t1 AT3G12700 27.682 466 273 19 5 436 26 461 8.85e-37 140
MS.gene92410.t1 AT1G79720 32.877 365 212 15 87 435 135 482 1.12e-34 135
MS.gene92410.t1 AT2G39710 29.787 376 213 15 89 430 67 425 3.97e-33 130
MS.gene92410.t1 AT5G37540 28.267 375 225 14 88 435 81 438 1.03e-31 126
MS.gene92410.t1 AT3G59080 30.311 386 209 14 72 438 155 499 1.24e-31 126
MS.gene92410.t1 AT1G66180 27.128 376 230 15 88 438 73 429 4.31e-30 121
MS.gene92410.t1 AT4G33490 25.316 395 236 13 78 438 45 414 3.00e-28 116
MS.gene92410.t1 AT5G02190 27.128 376 223 15 96 435 82 442 7.65e-28 115
MS.gene92410.t1 AT1G05840 28.000 375 234 15 85 436 78 439 2.08e-27 114
MS.gene92410.t1 AT5G43100 28.307 378 218 19 84 438 73 420 2.27e-27 114
MS.gene92410.t1 AT5G43100 28.307 378 218 19 84 438 73 420 4.30e-27 114
MS.gene92410.t1 AT3G54400 27.907 387 250 13 62 436 56 425 6.15e-27 112
MS.gene92410.t1 AT3G50050 26.124 467 265 22 12 438 13 439 1.45e-26 112
MS.gene92410.t1 AT1G44130 26.065 399 247 14 68 438 24 402 7.12e-26 108
MS.gene92410.t1 AT4G12920 26.234 385 246 12 61 435 31 387 8.70e-26 108
MS.gene92410.t1 AT5G45120 29.056 413 212 19 87 435 83 478 1.80e-25 108
MS.gene92410.t1 AT1G09750 28.997 369 222 16 85 435 102 448 3.59e-25 107
MS.gene92410.t1 AT2G36670 26.790 433 270 18 33 436 44 458 1.59e-23 103
MS.gene92410.t1 AT5G22850 28.232 379 229 14 85 436 79 441 1.75e-23 103
MS.gene92410.t1 AT2G36670 28.000 375 229 15 87 436 34 392 7.37e-23 100
MS.gene92410.t1 AT2G36670 28.000 375 229 15 87 436 34 392 7.37e-23 100
MS.gene92410.t1 AT1G77480 26.893 383 226 17 85 438 65 422 8.35e-23 100
MS.gene92410.t1 AT4G33490 29.167 288 163 11 78 349 42 304 8.99e-23 100
MS.gene92410.t1 AT1G77480 26.893 383 226 17 85 438 65 422 1.14e-22 100
MS.gene92410.t1 AT2G36670 28.117 377 226 16 87 436 105 463 1.16e-22 100
MS.gene92410.t1 AT2G17760 27.838 370 212 17 92 435 109 449 5.47e-21 95.9
MS.gene92410.t1 AT3G52500 28.043 460 256 23 37 436 24 468 6.87e-21 95.1
MS.gene92410.t1 AT5G07030 27.371 464 285 18 2 436 15 455 7.99e-21 94.7
MS.gene92410.t1 AT1G49050 23.077 364 233 12 105 437 50 397 9.07e-21 94.4
MS.gene92410.t1 AT1G49050 25.641 390 242 15 80 437 197 570 1.05e-20 95.1
MS.gene92410.t1 AT1G08210 27.851 377 230 15 85 436 130 489 1.97e-20 94.4
MS.gene92410.t1 AT1G08210 27.851 377 230 15 85 436 82 441 2.20e-20 94.0
MS.gene92410.t1 AT1G08210 27.851 377 230 15 85 436 116 475 2.82e-20 93.6
MS.gene92410.t1 AT3G51350 25.280 447 272 19 27 435 30 452 9.23e-19 89.0
MS.gene92410.t1 AT1G49050 27.957 279 168 12 80 341 197 459 7.42e-18 85.9
MS.gene92410.t1 AT3G25700 27.473 273 155 10 39 293 39 286 1.00e-16 81.6
MS.gene92410.t1 AT3G42550 24.384 365 209 12 87 436 78 390 1.28e-16 82.0
MS.gene92410.t1 AT1G08210 26.551 403 229 16 85 436 82 468 2.98e-16 81.3
MS.gene92410.t1 AT3G51340 23.967 363 234 13 92 435 108 447 3.57e-14 74.7
MS.gene92410.t1 AT5G24820 23.650 389 248 12 74 435 33 399 3.83e-13 71.2
MS.gene92410.t1 AT5G10080 24.635 479 280 27 10 437 7 455 1.38e-12 70.1
MS.gene92410.t1 AT3G51340 24.823 282 182 10 92 358 108 374 3.66e-11 65.1
MS.gene92410.t1 AT3G51340 24.555 281 182 10 92 357 108 373 6.42e-11 64.3
MS.gene92410.t1 AT3G51340 22.432 370 243 13 92 435 108 459 8.12e-11 64.3

Find 90 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGATGCCGCTGTGGTTTC+TGG 0.062205 8.4:-60176706 MS.gene92410:CDS
AACTGGTGTTTATAATAATT+TGG 0.122254 8.4:-60176512 MS.gene92410:CDS
TTAAAATGTGCCGTTATTAT+AGG 0.181038 8.4:+60176391 None:intergenic
GGTTCTGACCTTTCCATCTT+TGG 0.183882 8.4:-60176274 MS.gene92410:CDS
TTGATTGAGGTGTGTTTATA+AGG 0.192351 8.4:+60177268 None:intergenic
ACGACAAGCAAACTCAATTT+TGG 0.203851 8.4:-60176727 MS.gene92410:CDS
GGTTGGTATAGTGGTGATTT+TGG 0.223826 8.4:+60177369 None:intergenic
ACCCCACCATTTAAGTTATA+TGG 0.242744 8.4:-60177198 MS.gene92410:CDS
ACTGCAACAAATAAACTTAT+AGG 0.263470 8.4:+60176346 None:intergenic
GTCAACCCCTATAGTGAAAA+AGG 0.271435 8.4:-60176671 MS.gene92410:CDS
GCAATGCCATATAACTTAAA+TGG 0.273035 8.4:+60177192 None:intergenic
TCATTTCCTAAAACTGTGAT+AGG 0.290097 8.4:-60176901 MS.gene92410:CDS
GACAAAAGAATAGAATTTAA+AGG 0.318855 8.4:-60176601 MS.gene92410:CDS
CTTGTCGTGTTTGACTCAAC+CGG 0.337489 8.4:+60176742 None:intergenic
AGCGTCTCTTATTACACAAT+TGG 0.348748 8.4:-60176809 MS.gene92410:CDS
GGTAACATCATAATTGATTC+AGG 0.364855 8.4:-60176556 MS.gene92410:CDS
ATACAGGTAGTGACATTGTT+TGG 0.380232 8.4:-60177167 MS.gene92410:CDS
TATAGCAAAGGTTAAATTCT+CGG 0.388913 8.4:+60176440 None:intergenic
CTTAGCTCAGCAGAATTTGT+TGG 0.389893 8.4:-60176248 MS.gene92410:CDS
GCAGTTACTTCTGGGTTCCC+TGG 0.393771 8.4:-60176295 MS.gene92410:CDS
AGCTCTGGTGTAGTTGGCCT+TGG 0.395052 8.4:-60176841 MS.gene92410:CDS
GAGATGCCGCTGTGGTTTCT+GGG 0.406335 8.4:-60176705 MS.gene92410:CDS
GTCAAATAGTAGAAAACTAT+AGG 0.426026 8.4:+60176646 None:intergenic
GTGTTTGACTCAACCGGGTT+TGG 0.428845 8.4:+60176748 None:intergenic
GGAATCAGCTGTGTTGGAAT+TGG 0.432066 8.4:-60176491 MS.gene92410:CDS
ATAGCAAAGGTTAAATTCTC+GGG 0.433218 8.4:+60176441 None:intergenic
TCTGGTGTAGTTGGCCTTGG+AGG 0.438438 8.4:-60176838 MS.gene92410:CDS
ACGTTGACGGTTATTCCAAC+TGG 0.450671 8.4:-60176529 MS.gene92410:CDS
CTGAGCTAAGTTTCCAAAGA+TGG 0.451967 8.4:+60176261 None:intergenic
ATGAGATACTCACCATGATC+GGG 0.452999 8.4:+60177237 None:intergenic
GGTGTAGTTGGCCTTGGAGG+TGG 0.457901 8.4:-60176835 MS.gene92410:CDS
TGGTGGGGTACCAACTGAAT+AGG 0.458426 8.4:+60177212 None:intergenic
TGATTGAGGTGTGTTTATAA+GGG 0.459639 8.4:+60177269 None:intergenic
ACAAAAGAATAGAATTTAAA+GGG 0.460643 8.4:-60176600 MS.gene92410:CDS
GACAATACAATGTCATTTGA+AGG 0.463678 8.4:-60176868 MS.gene92410:CDS
AAAGAGAAATAATGAAACAA+AGG 0.467908 8.4:+60177442 None:intergenic
CCTAAAACTGTGATAGGATG+TGG 0.472735 8.4:-60176895 MS.gene92410:CDS
AAGAGAAATAATGAAACAAA+GGG 0.474915 8.4:+60177443 None:intergenic
GCCGCTGTGGTTTCTGGGGA+TGG 0.476800 8.4:-60176700 MS.gene92410:CDS
TAATTTGGAATCAGCTGTGT+TGG 0.477151 8.4:-60176497 MS.gene92410:CDS
AAATTATTATAAACACCAGT+TGG 0.480158 8.4:+60176514 None:intergenic
GCGTCTCTTATTACACAATT+GGG 0.481758 8.4:-60176808 MS.gene92410:CDS
TTATATGGCATTGCTGATAC+AGG 0.486441 8.4:-60177183 MS.gene92410:CDS
GAATTTAAAGGGTCTTCAAA+TGG 0.487908 8.4:-60176589 MS.gene92410:CDS
CTGATTGTAGCAAAGTGTAA+TGG 0.496814 8.4:-60176183 None:intergenic
GGTGCGAGCTCTGGTGTAGT+TGG 0.497588 8.4:-60176847 MS.gene92410:CDS
CTTGTTGCACAGATTTACAT+AGG 0.501571 8.4:+60177046 None:intergenic
TCATTTGAAGGTGCGAGCTC+TGG 0.505867 8.4:-60176856 MS.gene92410:CDS
TGCCATATAACTTAAATGGT+GGG 0.513388 8.4:+60177196 None:intergenic
TGCTATAGTGTCACATCAGA+TGG 0.514224 8.4:-60176424 MS.gene92410:CDS
GGTGATACGTCACAATCAAA+AGG 0.514921 8.4:-60176976 MS.gene92410:CDS
CAAAGGTTAAATTCTCGGGT+TGG 0.519243 8.4:+60176445 None:intergenic
TTATTGTTTGTTGCCAAACC+CGG 0.519426 8.4:-60176761 MS.gene92410:CDS
TGATTGTAACATTCTTTGCA+AGG 0.519730 8.4:+60177132 None:intergenic
TGATTCAGGTACAACGTTGA+CGG 0.527583 8.4:-60176542 MS.gene92410:CDS
TTGTCGTGTTTGACTCAACC+GGG 0.535482 8.4:+60176743 None:intergenic
CATGAGATACTCACCATGAT+CGG 0.536629 8.4:+60177236 None:intergenic
TTATTTGTTGCAGTTGCAGA+TGG 0.539859 8.4:-60176337 MS.gene92410:CDS
GTCTTCAAATGGTGGTGATG+AGG 0.541942 8.4:-60176578 MS.gene92410:CDS
TCAACTGTAATCCCCGATCA+TGG 0.547516 8.4:-60177249 MS.gene92410:CDS
CTTTCTCATGCTTTAAACAA+CGG 0.553785 8.4:-60177423 MS.gene92410:CDS
AAGATGGAAAGGTCAGAACC+AGG 0.560391 8.4:+60176277 None:intergenic
GCTCAGCAGAATTTGTTGGT+TGG 0.563160 8.4:-60176244 MS.gene92410:CDS
TCTTCAAATGGTGGTGATGA+GGG 0.564161 8.4:-60176577 MS.gene92410:CDS
ATGCCATATAACTTAAATGG+TGG 0.567034 8.4:+60177195 None:intergenic
TCGGGGATTACAGTTGATTG+AGG 0.568413 8.4:+60177255 None:intergenic
CTAAGTTTCCAAAGATGGAA+AGG 0.568814 8.4:+60176266 None:intergenic
GCCATATAACTTAAATGGTG+GGG 0.578953 8.4:+60177197 None:intergenic
GTGCGGCATTGACAATATGT+TGG 0.580612 8.4:+60177331 None:intergenic
AACAAAGGGAGAAGTAAAAG+AGG 0.583827 8.4:+60177457 None:intergenic
GAGACGCTGGTCCACCTCCA+AGG 0.588914 8.4:+60176824 None:intergenic
TTTAAAGGGTCTTCAAATGG+TGG 0.588943 8.4:-60176586 MS.gene92410:CDS
TACACTTTGCTACAATCAGT+AGG 0.589800 8.4:+60176187 None:intergenic
TGATGGTTTAAACTTAGGAG+TGG 0.593505 8.4:+60177107 None:intergenic
GATTGGCACGGTTTATAGAA+CGG 0.609697 8.4:+60177307 None:intergenic
ACATTGGAAGCATTTAGTGT+TGG 0.611142 8.4:-60176625 MS.gene92410:CDS
CTTACATTAGAGTCCTCCAC+TGG 0.621526 8.4:-60176934 MS.gene92410:CDS
AAATGAAATAGGACGTCCAG+TGG 0.627128 8.4:+60176918 None:intergenic
CTGATGTGACACTATAGCAA+AGG 0.629182 8.4:+60176428 None:intergenic
TTACATAGGTCAGACGAACA+TGG 0.633649 8.4:+60177060 None:intergenic
TATCTCATGACCTATTCAGT+TGG 0.633970 8.4:-60177222 MS.gene92410:CDS
AGATGGAAAGGTCAGAACCA+GGG 0.650286 8.4:+60176278 None:intergenic
TGAAATAGGACGTCCAGTGG+AGG 0.654304 8.4:+60176921 None:intergenic
AGATGCCGCTGTGGTTTCTG+GGG 0.659067 8.4:-60176704 MS.gene92410:CDS
TGAGATACTCACCATGATCG+GGG 0.668047 8.4:+60177238 None:intergenic
AATTGTGTAATAAGAGACGC+TGG 0.670336 8.4:+60176811 None:intergenic
CTATGTAAATCTGTGCAACA+AGG 0.670936 8.4:-60177045 MS.gene92410:CDS
ACGGTTTATAGAACGGCGTG+CGG 0.686698 8.4:+60177314 None:intergenic
GAAGTAAAAGAGGATCAGCA+AGG 0.691367 8.4:+60177467 None:intergenic
ACCATCCCCAGAAACCACAG+CGG 0.788719 8.4:+60176699 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATTGTCATTACAAT+CGG + chr8.4:60176649-60176668 None:intergenic 15.0%
!! ACAAAAGAATAGAATTTAAA+GGG - chr8.4:60177066-60177085 MS.gene92410:CDS 15.0%
!!! ATATGATTTTCCTATAATAA+CGG - chr8.4:60177265-60177284 MS.gene92410:CDS 15.0%
!!! TGTGAATATAGTATTTTTTA+TGG - chr8.4:60176669-60176688 MS.gene92410:CDS 15.0%
!! AAAGAGAAATAATGAAACAA+AGG + chr8.4:60176227-60176246 None:intergenic 20.0%
!! AAATTATTATAAACACCAGT+TGG + chr8.4:60177155-60177174 None:intergenic 20.0%
!! AACTGGTGTTTATAATAATT+TGG - chr8.4:60177154-60177173 MS.gene92410:CDS 20.0%
!! AAGAGAAATAATGAAACAAA+GGG + chr8.4:60176226-60176245 None:intergenic 20.0%
!! GACAAAAGAATAGAATTTAA+AGG - chr8.4:60177065-60177084 MS.gene92410:CDS 20.0%
! ACTGCAACAAATAAACTTAT+AGG + chr8.4:60177323-60177342 None:intergenic 25.0%
! GTCAAATAGTAGAAAACTAT+AGG + chr8.4:60177023-60177042 None:intergenic 25.0%
! TATAGCAAAGGTTAAATTCT+CGG + chr8.4:60177229-60177248 None:intergenic 25.0%
!! AGTTTTCTACTATTTGACAT+TGG - chr8.4:60177025-60177044 MS.gene92410:CDS 25.0%
!! ATAATAACGGCACATTTTAA+AGG - chr8.4:60177278-60177297 MS.gene92410:CDS 25.0%
!! TTAAAATGTGCCGTTATTAT+AGG + chr8.4:60177278-60177297 None:intergenic 25.0%
!!! ACAGTTTTAGGAAATGAAAT+AGG + chr8.4:60176762-60176781 None:intergenic 25.0%
!!! GATTTTGATGGTTTAAACTT+AGG + chr8.4:60176567-60176586 None:intergenic 25.0%
ATAGCAAAGGTTAAATTCTC+GGG + chr8.4:60177228-60177247 None:intergenic 30.0%
ATGCCATATAACTTAAATGG+TGG + chr8.4:60176474-60176493 None:intergenic 30.0%
GAATTTAAAGGGTCTTCAAA+TGG - chr8.4:60177077-60177096 MS.gene92410:CDS 30.0%
GCAATGCCATATAACTTAAA+TGG + chr8.4:60176477-60176496 None:intergenic 30.0%
TCATTTCCTAAAACTGTGAT+AGG - chr8.4:60176765-60176784 MS.gene92410:CDS 30.0%
TGATTGAGGTGTGTTTATAA+GGG + chr8.4:60176400-60176419 None:intergenic 30.0%
TGATTGTAACATTCTTTGCA+AGG + chr8.4:60176537-60176556 None:intergenic 30.0%
TGCCATATAACTTAAATGGT+GGG + chr8.4:60176473-60176492 None:intergenic 30.0%
TTGATTGAGGTGTGTTTATA+AGG + chr8.4:60176401-60176420 None:intergenic 30.0%
! CTTTCTCATGCTTTAAACAA+CGG - chr8.4:60176243-60176262 MS.gene92410:CDS 30.0%
! GACAATACAATGTCATTTGA+AGG - chr8.4:60176798-60176817 MS.gene92410:CDS 30.0%
! GGTAACATCATAATTGATTC+AGG - chr8.4:60177110-60177129 MS.gene92410:CDS 30.0%
! TTTTGTAGAAATGATTGGCA+CGG + chr8.4:60176374-60176393 None:intergenic 30.0%
!! ATAGGATCCTTTTTCACTAT+AGG + chr8.4:60177005-60177024 None:intergenic 30.0%
!! TAGGATCCTTTTTCACTATA+GGG + chr8.4:60177004-60177023 None:intergenic 30.0%
!! TTGTAAGTTGACGATTTTGA+TGG + chr8.4:60176579-60176598 None:intergenic 30.0%
!!! TGTTGGTATTTGTTTTGTGT+AGG + chr8.4:60176321-60176340 None:intergenic 30.0%
AACAAAGGGAGAAGTAAAAG+AGG + chr8.4:60176212-60176231 None:intergenic 35.0%
AATTGTGTAATAAGAGACGC+TGG + chr8.4:60176858-60176877 None:intergenic 35.0%
AGCGTCTCTTATTACACAAT+TGG - chr8.4:60176857-60176876 MS.gene92410:CDS 35.0%
ATACAGGTAGTGACATTGTT+TGG - chr8.4:60176499-60176518 MS.gene92410:CDS 35.0%
CTAAGTTTCCAAAGATGGAA+AGG + chr8.4:60177403-60177422 None:intergenic 35.0%
CTATGTAAATCTGTGCAACA+AGG - chr8.4:60176621-60176640 MS.gene92410:CDS 35.0%
CTTGTTGCACAGATTTACAT+AGG + chr8.4:60176623-60176642 None:intergenic 35.0%
GCCATATAACTTAAATGGTG+GGG + chr8.4:60176472-60176491 None:intergenic 35.0%
GCGTCTCTTATTACACAATT+GGG - chr8.4:60176858-60176877 MS.gene92410:CDS 35.0%
TACACTTTGCTACAATCAGT+AGG + chr8.4:60177482-60177501 None:intergenic 35.0%
TATCTCATGACCTATTCAGT+TGG - chr8.4:60176444-60176463 MS.gene92410:CDS 35.0%
TGATGGTTTAAACTTAGGAG+TGG + chr8.4:60176562-60176581 None:intergenic 35.0%
TTATATGGCATTGCTGATAC+AGG - chr8.4:60176483-60176502 MS.gene92410:CDS 35.0%
TTTAAAGGGTCTTCAAATGG+TGG - chr8.4:60177080-60177099 MS.gene92410:CDS 35.0%
! ACCCCACCATTTAAGTTATA+TGG - chr8.4:60176468-60176487 MS.gene92410:CDS 35.0%
! ACGACAAGCAAACTCAATTT+TGG - chr8.4:60176939-60176958 MS.gene92410:CDS 35.0%
! ACGACACAATTTTTCGTTGA+AGG + chr8.4:60177454-60177473 None:intergenic 35.0%
! GTTGGTATAGTGGTGATTTT+GGG + chr8.4:60176299-60176318 None:intergenic 35.0%
! TAATTTGGAATCAGCTGTGT+TGG - chr8.4:60177169-60177188 MS.gene92410:CDS 35.0%
! TTATTGTTTGTTGCCAAACC+CGG - chr8.4:60176905-60176924 MS.gene92410:CDS 35.0%
! TTATTTGTTGCAGTTGCAGA+TGG - chr8.4:60177329-60177348 MS.gene92410:CDS 35.0%
! TTGGTATAGTGGTGATTTTG+GGG + chr8.4:60176298-60176317 None:intergenic 35.0%
!! ACATTGGAAGCATTTAGTGT+TGG - chr8.4:60177041-60177060 MS.gene92410:CDS 35.0%
!! AGGATCCTTTTTCACTATAG+GGG + chr8.4:60177003-60177022 None:intergenic 35.0%
!! GGCAGTTTTGTAGAAATGAT+TGG + chr8.4:60176379-60176398 None:intergenic 35.0%
!! TTGCTTTTGCAGTTACTTCT+GGG - chr8.4:60177363-60177382 MS.gene92410:CDS 35.0%
!! TTTGCTTTTGCAGTTACTTC+TGG - chr8.4:60177362-60177381 MS.gene92410:CDS 35.0%
!!! GGTATTTGTTTTGTGTAGGT+TGG + chr8.4:60176317-60176336 None:intergenic 35.0%
!!! TTTTGTGTAGGTTGGTATAG+TGG + chr8.4:60176309-60176328 None:intergenic 35.0%
AAATGAAATAGGACGTCCAG+TGG + chr8.4:60176751-60176770 None:intergenic 40.0%
ATGAGATACTCACCATGATC+GGG + chr8.4:60176432-60176451 None:intergenic 40.0%
CAAAGGTTAAATTCTCGGGT+TGG + chr8.4:60177224-60177243 None:intergenic 40.0%
CATGAGATACTCACCATGAT+CGG + chr8.4:60176433-60176452 None:intergenic 40.0%
CCTAAAACTGTGATAGGATG+TGG - chr8.4:60176771-60176790 MS.gene92410:CDS 40.0%
CTGAGCTAAGTTTCCAAAGA+TGG + chr8.4:60177408-60177427 None:intergenic 40.0%
CTGATGTGACACTATAGCAA+AGG + chr8.4:60177241-60177260 None:intergenic 40.0%
CTTAGCTCAGCAGAATTTGT+TGG - chr8.4:60177418-60177437 MS.gene92410:CDS 40.0%
GAAGTAAAAGAGGATCAGCA+AGG + chr8.4:60176202-60176221 None:intergenic 40.0%
GATTGGCACGGTTTATAGAA+CGG + chr8.4:60176362-60176381 None:intergenic 40.0%
GGTGATACGTCACAATCAAA+AGG - chr8.4:60176690-60176709 MS.gene92410:CDS 40.0%
GTCAACCCCTATAGTGAAAA+AGG - chr8.4:60176995-60177014 MS.gene92410:CDS 40.0%
TGATTCAGGTACAACGTTGA+CGG - chr8.4:60177124-60177143 MS.gene92410:CDS 40.0%
TGCTATAGTGTCACATCAGA+TGG - chr8.4:60177242-60177261 MS.gene92410:CDS 40.0%
TTACATAGGTCAGACGAACA+TGG + chr8.4:60176609-60176628 None:intergenic 40.0%
! CCACATCCTATCACAGTTTT+AGG + chr8.4:60176774-60176793 None:intergenic 40.0%
! TCTTCAAATGGTGGTGATGA+GGG - chr8.4:60177089-60177108 MS.gene92410:CDS 40.0%
!! GGTTGGTATAGTGGTGATTT+TGG + chr8.4:60176300-60176319 None:intergenic 40.0%
AAGATGGAAAGGTCAGAACC+AGG + chr8.4:60177392-60177411 None:intergenic 45.0%
ACGTTGACGGTTATTCCAAC+TGG - chr8.4:60177137-60177156 MS.gene92410:CDS 45.0%
AGATGGAAAGGTCAGAACCA+GGG + chr8.4:60177391-60177410 None:intergenic 45.0%
CTTACATTAGAGTCCTCCAC+TGG - chr8.4:60176732-60176751 MS.gene92410:CDS 45.0%
CTTGTCGTGTTTGACTCAAC+CGG + chr8.4:60176927-60176946 None:intergenic 45.0%
GGAATCAGCTGTGTTGGAAT+TGG - chr8.4:60177175-60177194 MS.gene92410:CDS 45.0%
TCAACTGTAATCCCCGATCA+TGG - chr8.4:60176417-60176436 MS.gene92410:CDS 45.0%
TCGGGGATTACAGTTGATTG+AGG + chr8.4:60176414-60176433 None:intergenic 45.0%
TGAGATACTCACCATGATCG+GGG + chr8.4:60176431-60176450 None:intergenic 45.0%
TTGTCGTGTTTGACTCAACC+GGG + chr8.4:60176926-60176945 None:intergenic 45.0%
! GGTTCTGACCTTTCCATCTT+TGG - chr8.4:60177392-60177411 MS.gene92410:CDS 45.0%
! GTCTTCAAATGGTGGTGATG+AGG - chr8.4:60177088-60177107 MS.gene92410:CDS 45.0%
!! GCTCAGCAGAATTTGTTGGT+TGG - chr8.4:60177422-60177441 MS.gene92410:CDS 45.0%
!! GTGCGGCATTGACAATATGT+TGG + chr8.4:60176338-60176357 None:intergenic 45.0%
ACGGTTTATAGAACGGCGTG+CGG + chr8.4:60176355-60176374 None:intergenic 50.0%
GTGTTTGACTCAACCGGGTT+TGG + chr8.4:60176921-60176940 None:intergenic 50.0%
TGAAATAGGACGTCCAGTGG+AGG + chr8.4:60176748-60176767 None:intergenic 50.0%
! TCATTTGAAGGTGCGAGCTC+TGG - chr8.4:60176810-60176829 MS.gene92410:CDS 50.0%
! TGGTGGGGTACCAACTGAAT+AGG + chr8.4:60176457-60176476 None:intergenic 50.0%
!! CAATTTTGGAGATGCCGCTG+TGG - chr8.4:60176953-60176972 MS.gene92410:CDS 50.0%
ACCATCCCCAGAAACCACAG+CGG + chr8.4:60176970-60176989 None:intergenic 55.0%
AGATGCCGCTGTGGTTTCTG+GGG - chr8.4:60176962-60176981 MS.gene92410:CDS 55.0%
GAGATGCCGCTGTGGTTTCT+GGG - chr8.4:60176961-60176980 MS.gene92410:CDS 55.0%
GCAGTTACTTCTGGGTTCCC+TGG - chr8.4:60177371-60177390 MS.gene92410:CDS 55.0%
! AGCTCTGGTGTAGTTGGCCT+TGG - chr8.4:60176825-60176844 MS.gene92410:CDS 55.0%
!! GTGGTGATTTTGGGGAGTCG+CGG + chr8.4:60176290-60176309 None:intergenic 55.0%
!! TCTGGTGTAGTTGGCCTTGG+AGG - chr8.4:60176828-60176847 MS.gene92410:CDS 55.0%
GGAGATGCCGCTGTGGTTTC+TGG - chr8.4:60176960-60176979 MS.gene92410:CDS 60.0%
! GGTGCGAGCTCTGGTGTAGT+TGG - chr8.4:60176819-60176838 MS.gene92410:CDS 60.0%
!! GGTGTAGTTGGCCTTGGAGG+TGG - chr8.4:60176831-60176850 MS.gene92410:CDS 60.0%
!! GTGATTTTGGGGAGTCGCGG+TGG + chr8.4:60176287-60176306 None:intergenic 60.0%
GAGACGCTGGTCCACCTCCA+AGG + chr8.4:60176845-60176864 None:intergenic 65.0%
! GCCGCTGTGGTTTCTGGGGA+TGG - chr8.4:60176966-60176985 MS.gene92410:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 60176186 60177502 60176186 ID=MS.gene92410
chr8.4 mRNA 60176186 60177502 60176186 ID=MS.gene92410.t1;Parent=MS.gene92410
chr8.4 exon 60176186 60177502 60176186 ID=MS.gene92410.t1.exon1;Parent=MS.gene92410.t1
chr8.4 CDS 60176186 60177502 60176186 ID=cds.MS.gene92410.t1;Parent=MS.gene92410.t1
Gene Sequence

>MS.gene92410

ATGAATATATGTTCCTTGCTGATCCTCTTTTACTTCTCCCTTTGTTTCATTATTTCTCTTTCTCATGCTTTAAACAACGGTTTTAGTGTTGAACTCATCCACCGCGACTCCCCAAAATCACCACTATACCAACCTACACAAAACAAATACCAACATATTGTCAATGCCGCACGCCGTTCTATAAACCGTGCCAATCATTTCTACAAAACTGCCCTTATAAACACACCTCAATCAACTGTAATCCCCGATCATGGTGAGTATCTCATGACCTATTCAGTTGGTACCCCACCATTTAAGTTATATGGCATTGCTGATACAGGTAGTGACATTGTTTGGCTTCAATGCGAGCCTTGCAAAGAATGTTACAATCAAACCACTCCTAAGTTTAAACCATCAAAATCGTCAACTTACAAAAACATTCCATGTTCGTCTGACCTATGTAAATCTGTGCAACAAGGTGCCGATTGTAATGACAATTTTTTTTGTGAATATAGTATTTTTTATGGTGATACGTCACAATCAAAAGGAAATCTTAGTGTCGATACTCTTACATTAGAGTCCTCCACTGGACGTCCTATTTCATTTCCTAAAACTGTGATAGGATGTGGAACTGACAATACAATGTCATTTGAAGGTGCGAGCTCTGGTGTAGTTGGCCTTGGAGGTGGACCAGCGTCTCTTATTACACAATTGGGATCATCAATTGATTCAAAATTCTCTTATTGTTTGTTGCCAAACCCGGTTGAGTCAAACACGACAAGCAAACTCAATTTTGGAGATGCCGCTGTGGTTTCTGGGGATGGTGTAGTGTCAACCCCTATAGTGAAAAAGGATCCTATAGTTTTCTACTATTTGACATTGGAAGCATTTAGTGTTGGTGACAAAAGAATAGAATTTAAAGGGTCTTCAAATGGTGGTGATGAGGGTAACATCATAATTGATTCAGGTACAACGTTGACGGTTATTCCAACTGGTGTTTATAATAATTTGGAATCAGCTGTGTTGGAATTGGTGAAACTGAAGCGTGTTGATGATCCAACCCGAGAATTTAACCTTTGCTATAGTGTCACATCAGATGGATATGATTTTCCTATAATAACGGCACATTTTAAAGGTGCAGATGTTAAGTTGCATCCTATAAGTTTATTTGTTGCAGTTGCAGATGGCGTTGTTTGCTTTGCTTTTGCAGTTACTTCTGGGTTCCCTGGTTCTGACCTTTCCATCTTTGGAAACTTAGCTCAGCAGAATTTGTTGGTTGGTTATGACCTTCAACGAAAAATTGTGTCGTTTAAGCCTACTGATTGTAGCAAAGTGTAA
Protein sequence

>MS.gene92410.t1

MNICSLLILFYFSLCFIISLSHALNNGFSVELIHRDSPKSPLYQPTQNKYQHIVNAARRSINRANHFYKTALINTPQSTVIPDHGEYLMTYSVGTPPFKLYGIADTGSDIVWLQCEPCKECYNQTTPKFKPSKSSTYKNIPCSSDLCKSVQQGADCNDNFFCEYSIFYGDTSQSKGNLSVDTLTLESSTGRPISFPKTVIGCGTDNTMSFEGASSGVVGLGGGPASLITQLGSSIDSKFSYCLLPNPVESNTTSKLNFGDAAVVSGDGVVSTPIVKKDPIVFYYLTLEAFSVGDKRIEFKGSSNGGDEGNIIIDSGTTLTVIPTGVYNNLESAVLELVKLKRVDDPTREFNLCYSVTSDGYDFPIITAHFKGADVKLHPISLFVAVADGVVCFAFAVTSGFPGSDLSIFGNLAQQNLLVGYDLQRKIVSFKPTDCSKV