AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92414

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92414.t1 MTR_8g038310 93.056 432 13 2 1 432 1 415 0.0 813
MS.gene92414.t1 MTR_8g096990 66.513 433 140 4 1 430 1 431 0.0 568
MS.gene92414.t1 MTR_5g044770 64.896 433 146 4 1 430 1 430 0.0 563
MS.gene92414.t1 MTR_5g080960 63.448 435 152 4 1 430 1 433 0.0 557
MS.gene92414.t1 MTR_5g080890 62.759 435 155 4 1 430 1 433 0.0 552
MS.gene92414.t1 MTR_8g038280 63.387 437 151 4 1 430 1 435 0.0 552
MS.gene92414.t1 MTR_8g038250 62.642 439 131 6 1 430 1 415 0.0 535
MS.gene92414.t1 MTR_6g090290 60.731 438 162 5 1 430 1 436 3.96e-175 497
MS.gene92414.t1 MTR_4g109760 57.044 433 148 7 1 430 1 398 3.12e-149 430
MS.gene92414.t1 MTR_3g028120 47.045 423 214 5 18 430 16 438 1.56e-134 394
MS.gene92414.t1 MTR_5g038360 46.154 429 217 7 14 430 10 436 2.30e-131 385
MS.gene92414.t1 MTR_6g088760 53.012 415 180 8 1 404 1 411 1.92e-130 386
MS.gene92414.t1 MTR_2g067830 52.696 408 181 9 33 430 1 406 5.73e-127 373
MS.gene92414.t1 MTR_0024s0040 46.973 413 203 7 31 430 1 410 6.44e-121 358
MS.gene92414.t1 MTR_5g044570 44.875 439 196 11 5 430 6 411 9.41e-120 355
MS.gene92414.t1 MTR_2g105300 48.148 405 176 11 33 427 1 381 3.66e-110 329
MS.gene92414.t1 MTR_0036s0050 41.216 444 234 12 4 430 2 435 3.49e-98 301
MS.gene92414.t1 MTR_0036s0070 39.498 438 241 10 4 430 2 426 6.17e-98 300
MS.gene92414.t1 MTR_0036s0110 40.133 451 240 11 1 430 1 442 6.84e-98 300
MS.gene92414.t1 MTR_2g059490 40.909 440 220 10 1 430 1 410 6.21e-96 295
MS.gene92414.t1 MTR_5g008070 40.767 417 224 9 22 429 23 425 1.02e-95 294
MS.gene92414.t1 MTR_2g059460 40.865 416 203 11 24 430 25 406 1.23e-90 280
MS.gene92414.t1 MTR_5g008150 41.361 382 208 8 60 430 36 412 9.75e-88 273
MS.gene92414.t1 MTR_5g008110 37.130 439 229 9 2 430 14 415 6.15e-87 271
MS.gene92414.t1 MTR_3g097150 35.971 417 243 9 28 430 42 448 1.66e-73 237
MS.gene92414.t1 MTR_1g060750 37.065 402 214 11 41 430 2 376 6.75e-71 228
MS.gene92414.t1 MTR_4g045857 41.667 312 145 8 7 311 7 288 2.61e-65 211
MS.gene92414.t1 MTR_5g008090 38.462 299 168 8 143 430 21 314 1.26e-54 184
MS.gene92414.t1 MTR_1g115210 33.333 426 253 13 22 428 73 486 7.29e-53 184
MS.gene92414.t1 MTR_1g039270 32.227 422 248 14 32 428 61 469 1.95e-50 177
MS.gene92414.t1 MTR_7g117430 33.488 430 240 16 26 428 74 484 8.06e-50 176
MS.gene92414.t1 MTR_1g111350 30.602 415 251 11 22 428 82 467 9.07e-45 162
MS.gene92414.t1 MTR_6g085010 32.853 347 217 8 87 428 156 491 3.94e-44 160
MS.gene92414.t1 MTR_3g046300 32.898 383 235 11 69 430 79 460 2.72e-41 152
MS.gene92414.t1 MTR_2g028190 32.609 368 222 13 78 428 93 451 4.26e-41 152
MS.gene92414.t1 MTR_4g007740 31.910 398 240 14 50 429 94 478 7.98e-40 149
MS.gene92414.t1 MTR_6g088765 39.167 240 113 4 163 401 5 212 1.36e-39 142
MS.gene92414.t1 MTR_8g103880 29.167 384 250 13 55 428 84 455 1.48e-38 145
MS.gene92414.t1 MTR_3g053710 32.778 360 216 12 86 428 116 466 7.87e-38 143
MS.gene92414.t1 MTR_5g091130 32.984 382 233 11 69 428 173 553 1.39e-37 144
MS.gene92414.t1 MTR_3g068000 29.659 381 211 14 90 428 76 441 7.11e-34 132
MS.gene92414.t1 MTR_2g070430 27.609 460 285 17 2 425 3 450 3.00e-33 130
MS.gene92414.t1 MTR_2g055840 29.155 367 227 12 88 428 14 373 4.02e-32 125
MS.gene92414.t1 MTR_7g062360 28.267 375 222 14 90 428 62 425 2.17e-29 119
MS.gene92414.t1 MTR_7g105850 27.495 491 259 22 6 430 8 467 7.83e-29 118
MS.gene92414.t1 MTR_4g008110 53.153 111 31 1 162 272 48 137 7.74e-28 107
MS.gene92414.t1 MTR_3g065160 26.287 369 229 12 89 430 76 428 1.08e-26 111
MS.gene92414.t1 MTR_3g053980 32.022 356 218 11 86 428 141 485 2.73e-26 111
MS.gene92414.t1 MTR_2g063110 25.000 488 254 19 29 428 45 508 8.13e-26 109
MS.gene92414.t1 MTR_4g095270 28.032 371 217 17 86 430 79 425 8.81e-26 110
MS.gene92414.t1 MTR_4g095270 28.032 371 217 17 86 430 79 425 9.68e-26 110
MS.gene92414.t1 MTR_6g084710 25.066 379 236 13 86 431 190 553 1.03e-24 107
MS.gene92414.t1 MTR_1g037630 28.941 425 260 16 26 429 26 429 1.03e-24 105
MS.gene92414.t1 MTR_5g016260 26.230 366 230 15 86 430 87 433 1.61e-24 106
MS.gene92414.t1 MTR_5g016260 26.230 366 230 15 86 430 87 433 1.85e-24 106
MS.gene92414.t1 MTR_1g078240 27.078 373 233 14 86 429 68 430 2.46e-23 102
MS.gene92414.t1 MTR_8g075090 27.151 372 219 17 88 430 113 461 4.40e-23 102
MS.gene92414.t1 MTR_5g022880 27.248 367 223 14 88 428 113 461 5.66e-23 101
MS.gene92414.t1 MTR_3g014290 29.972 357 215 13 88 429 81 417 7.78e-23 100
MS.gene92414.t1 MTR_8g075090 27.606 355 209 16 88 417 113 444 8.75e-23 100
MS.gene92414.t1 MTR_1g078240 27.733 375 229 16 86 429 68 431 3.14e-22 99.0
MS.gene92414.t1 MTR_8g039540 28.285 449 274 19 10 429 5 434 7.79e-22 97.4
MS.gene92414.t1 MTR_6g084710 26.101 318 202 11 86 385 190 492 8.53e-22 97.8
MS.gene92414.t1 MTR_5g077850 27.005 374 224 12 89 430 83 439 1.20e-21 97.1
MS.gene92414.t1 MTR_7g080230 25.629 437 267 17 33 429 13 431 2.39e-21 96.3
MS.gene92414.t1 MTR_2g103340 41.880 117 24 1 21 137 33 105 2.56e-21 91.7
MS.gene92414.t1 MTR_8g094590 33.205 259 143 13 86 334 72 310 3.44e-21 94.0
MS.gene92414.t1 MTR_8g094590 30.961 281 162 14 86 354 72 332 4.52e-21 95.1
MS.gene92414.t1 MTR_8g094590 30.961 281 162 14 86 354 72 332 4.58e-21 95.1
MS.gene92414.t1 MTR_1g078240 29.316 307 187 12 86 372 68 364 1.20e-20 93.6
MS.gene92414.t1 MTR_8g075090 28.339 307 180 13 88 373 113 400 1.98e-20 93.6
MS.gene92414.t1 MTR_2g084550 27.005 374 232 14 86 429 76 438 4.08e-20 92.8
MS.gene92414.t1 MTR_4g056420 27.056 377 233 16 83 429 73 437 5.62e-20 92.4
MS.gene92414.t1 MTR_5g061690 27.479 353 201 19 94 417 108 434 5.63e-19 89.7
MS.gene92414.t1 MTR_0945s0010 37.903 124 72 3 310 429 1 123 7.04e-19 84.0
MS.gene92414.t1 MTR_3g096930 28.723 282 164 14 86 353 83 341 1.02e-18 88.2
MS.gene92414.t1 MTR_7g021510 24.211 380 236 15 86 429 71 434 1.13e-18 88.6
MS.gene92414.t1 MTR_7g102750 25.134 374 238 15 86 429 80 441 3.16e-18 87.0
MS.gene92414.t1 MTR_1g029810 24.866 374 234 16 86 430 56 411 5.86e-18 85.9
MS.gene92414.t1 MTR_1g029810 24.866 374 234 16 86 430 56 411 7.57e-18 85.9
MS.gene92414.t1 MTR_1g061940 25.600 375 237 17 86 429 82 445 8.91e-18 85.9
MS.gene92414.t1 MTR_4g023650 26.667 405 231 18 67 430 42 421 1.52e-17 84.7
MS.gene92414.t1 MTR_3g087780 26.989 352 207 17 94 417 106 435 1.67e-16 82.0
MS.gene92414.t1 MTR_4g100990 25.307 407 229 17 87 428 74 470 2.57e-16 81.3
MS.gene92414.t1 MTR_5g080330 24.438 356 203 13 104 428 2 322 6.12e-16 79.7
MS.gene92414.t1 MTR_7g102750 26.909 275 168 10 86 338 80 343 1.06e-15 79.3
MS.gene92414.t1 MTR_3g100500 29.064 406 243 19 41 421 45 430 5.53e-15 77.0
MS.gene92414.t1 MTR_5g077920 23.077 377 237 12 89 430 70 428 2.01e-14 75.1
MS.gene92414.t1 MTR_4g023630 24.000 400 248 14 67 430 42 421 2.86e-14 74.7
MS.gene92414.t1 MTR_5g061690 26.846 298 174 16 139 417 23 295 2.66e-13 71.6
MS.gene92414.t1 MTR_7g080230 25.648 347 217 14 111 429 28 361 8.75e-13 70.1
MS.gene92414.t1 MTR_4g117090 20.202 396 247 11 87 426 49 431 1.11e-12 70.1
MS.gene92414.t1 MTR_3g006820 23.545 378 236 19 86 430 57 414 1.20e-11 66.6
MS.gene92414.t1 MTR_3g087780 26.471 306 186 15 130 417 9 293 3.96e-11 64.7
MS.gene92414.t1 MTR_2g037710 27.184 206 124 9 231 428 18 205 5.89e-11 62.4
MS.gene92414.t1 MTR_3g087780 26.471 306 186 15 130 417 45 329 6.18e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92414.t1 AT1G64830 48.166 409 205 6 27 430 24 430 7.34e-126 371
MS.gene92414.t1 AT5G33340 48.900 409 201 7 28 430 30 436 2.47e-122 363
MS.gene92414.t1 AT2G35615 43.213 442 230 10 7 429 5 444 2.75e-105 319
MS.gene92414.t1 AT1G31450 43.371 445 233 8 1 429 1 442 1.68e-101 310
MS.gene92414.t1 AT2G28010 37.813 439 222 11 1 432 1 395 6.77e-81 255
MS.gene92414.t1 AT2G28220 37.585 439 223 11 1 429 1 398 2.01e-77 246
MS.gene92414.t1 AT2G28030 41.972 355 180 9 84 432 57 391 2.65e-75 240
MS.gene92414.t1 AT2G28225 36.219 439 229 11 1 429 1 398 1.01e-71 231
MS.gene92414.t1 AT2G28040 37.529 437 219 15 1 429 1 391 2.61e-71 230
MS.gene92414.t1 AT2G28040 38.537 410 199 14 27 429 19 382 1.25e-69 225
MS.gene92414.t1 AT2G03200 35.253 434 237 16 26 430 42 460 4.70e-63 211
MS.gene92414.t1 AT2G23945 28.821 458 273 16 9 430 10 450 4.40e-48 171
MS.gene92414.t1 AT3G20015 30.353 425 258 13 29 428 59 470 1.51e-47 169
MS.gene92414.t1 AT4G30030 34.181 354 208 12 88 428 78 419 2.52e-47 168
MS.gene92414.t1 AT1G25510 33.105 438 248 16 22 428 60 483 1.53e-45 164
MS.gene92414.t1 AT5G10760 34.152 407 229 15 32 428 87 464 3.65e-45 163
MS.gene92414.t1 AT1G01300 33.100 429 242 15 29 428 72 484 7.58e-45 162
MS.gene92414.t1 AT3G18490 30.404 421 271 14 19 428 91 500 5.87e-44 160
MS.gene92414.t1 AT4G30040 31.556 450 242 21 9 423 9 427 5.95e-42 154
MS.gene92414.t1 AT5G10770 32.227 422 248 16 25 429 74 474 5.04e-41 152
MS.gene92414.t1 AT3G61820 32.143 392 231 12 56 428 108 483 3.48e-39 147
MS.gene92414.t1 AT3G12700 26.411 443 303 11 4 429 25 461 4.50e-39 146
MS.gene92414.t1 AT3G25700 29.302 430 250 17 40 431 39 452 1.12e-38 145
MS.gene92414.t1 AT1G79720 30.541 370 206 13 88 428 135 482 1.26e-36 140
MS.gene92414.t1 AT3G59080 31.266 387 226 14 73 428 155 532 8.00e-34 133
MS.gene92414.t1 AT2G42980 31.116 421 246 15 43 428 113 524 1.80e-33 132
MS.gene92414.t1 AT2G39710 28.224 411 220 19 66 423 37 425 3.88e-31 124
MS.gene92414.t1 AT4G12920 28.954 373 231 15 68 428 37 387 2.57e-29 118
MS.gene92414.t1 AT5G02190 28.150 373 215 14 97 428 82 442 2.88e-29 119
MS.gene92414.t1 AT1G66180 28.302 371 222 14 89 430 73 428 3.84e-29 118
MS.gene92414.t1 AT5G37540 28.877 374 216 15 89 428 81 438 1.51e-27 114
MS.gene92414.t1 AT4G33490 26.344 372 231 14 86 430 58 413 8.98e-27 111
MS.gene92414.t1 AT3G54400 28.095 420 256 16 31 429 31 425 1.04e-26 111
MS.gene92414.t1 AT5G43100 27.671 365 223 15 86 429 74 418 1.15e-26 112
MS.gene92414.t1 AT5G43100 28.219 365 221 16 86 429 74 418 1.26e-26 113
MS.gene92414.t1 AT5G36260 25.936 374 232 15 86 429 76 434 1.77e-26 111
MS.gene92414.t1 AT3G59080 29.581 382 203 14 73 428 155 496 1.45e-25 109
MS.gene92414.t1 AT3G02740 27.174 368 234 13 86 429 83 440 9.76e-25 106
MS.gene92414.t1 AT3G02740 26.776 366 234 14 88 429 10 365 1.40e-24 105
MS.gene92414.t1 AT3G52500 29.574 399 208 20 86 429 88 468 2.24e-24 105
MS.gene92414.t1 AT4G33490 29.856 278 170 11 86 354 55 316 6.40e-24 103
MS.gene92414.t1 AT1G09750 27.624 362 228 13 86 428 102 448 9.95e-24 103
MS.gene92414.t1 AT3G50050 26.386 451 275 22 12 430 13 438 1.34e-23 103
MS.gene92414.t1 AT5G45120 28.607 402 220 18 88 428 83 478 9.47e-23 100
MS.gene92414.t1 AT2G17760 27.294 425 251 22 33 428 54 449 6.98e-22 98.2
MS.gene92414.t1 AT1G65240 26.757 370 231 17 86 429 72 427 7.70e-21 95.1
MS.gene92414.t1 AT1G49050 23.546 361 231 13 105 431 49 398 8.35e-21 94.4
MS.gene92414.t1 AT1G49050 23.546 361 231 13 105 431 222 571 2.14e-20 94.0
MS.gene92414.t1 AT5G10080 24.783 460 278 20 12 424 7 445 2.19e-20 94.0
MS.gene92414.t1 AT1G08210 25.000 372 239 14 86 429 82 441 6.92e-20 92.0
MS.gene92414.t1 AT1G08210 25.269 372 238 14 86 429 130 489 7.03e-20 92.4
MS.gene92414.t1 AT1G08210 25.000 372 239 14 86 429 116 475 7.19e-20 92.4
MS.gene92414.t1 AT2G36670 24.709 429 265 18 46 429 43 458 7.39e-20 92.0
MS.gene92414.t1 AT2G36670 24.528 371 239 14 88 429 34 392 3.33e-19 89.7
MS.gene92414.t1 AT2G36670 24.528 371 239 14 88 429 34 392 3.33e-19 89.7
MS.gene92414.t1 AT2G36670 24.528 371 239 14 88 429 105 463 3.58e-19 90.1
MS.gene92414.t1 AT1G05840 24.457 368 248 10 86 429 78 439 3.86e-19 90.1
MS.gene92414.t1 AT5G22850 25.867 375 235 15 86 429 79 441 7.35e-19 89.0
MS.gene92414.t1 AT3G42550 23.269 361 210 11 88 429 78 390 6.89e-18 85.9
MS.gene92414.t1 AT3G25700 31.148 183 103 6 40 208 39 212 1.41e-17 84.0
MS.gene92414.t1 AT3G51350 26.136 352 222 16 88 417 102 437 4.05e-17 84.0
MS.gene92414.t1 AT1G08210 28.205 273 165 9 86 338 82 343 1.00e-16 82.8
MS.gene92414.t1 AT5G07030 27.002 437 262 19 7 409 22 435 6.88e-16 79.7
MS.gene92414.t1 AT3G51360 25.714 350 216 15 93 419 10 338 2.45e-15 77.8
MS.gene92414.t1 AT3G51360 25.714 350 216 15 93 419 104 432 2.63e-15 78.2
MS.gene92414.t1 AT3G51360 25.714 350 216 15 93 419 94 422 2.65e-15 78.2
MS.gene92414.t1 AT4G35880 25.090 279 176 10 94 356 113 374 3.13e-15 77.8
MS.gene92414.t1 AT3G51360 25.439 342 212 14 93 411 104 425 1.72e-13 72.4
MS.gene92414.t1 AT3G51360 25.439 342 212 14 93 411 94 415 1.93e-13 72.4
MS.gene92414.t1 AT5G24820 25.172 290 191 8 156 432 127 403 5.40e-12 67.8
MS.gene92414.t1 AT3G51360 25.641 312 195 12 93 387 104 395 6.11e-12 67.4
MS.gene92414.t1 AT3G51360 25.641 312 195 12 93 387 94 385 8.66e-12 67.0
MS.gene92414.t1 AT3G51330 26.690 281 175 12 93 356 107 373 5.27e-11 64.7

Find 95 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCCCGCCATTTAAGGTATT+TGG 0.135768 8.4:-60139681 MS.gene92414:CDS
GGAGATGCCGCGGTGGTTTA+TGG 0.172384 8.4:-60139198 MS.gene92414:CDS
GATTGGTACCCCGCCATTTA+AGG 0.190570 8.4:-60139688 MS.gene92414:CDS
GGAGACAAATTAATAGAATT+TGG 0.199240 8.4:-60139096 MS.gene92414:CDS
TTAAAATGTGCAGTGATTAA+AGG 0.202169 8.4:+60138880 None:intergenic
TGGAAAATTCTCTTATTGTT+TGG 0.217865 8.4:-60139259 MS.gene92414:CDS
TGAATATACTTCTAAGTATA+TGG 0.237270 8.4:-60139481 MS.gene92414:CDS
GTCAAATATAGGTGTGATTT+GGG 0.239189 8.4:+60139596 None:intergenic
GGAAAATTCTCTTATTGTTT+GGG 0.247732 8.4:-60139258 MS.gene92414:CDS
ACAATGCCAAATACCTTAAA+TGG 0.265340 8.4:+60139675 None:intergenic
TGCTGATGGGATTGCTTGCT+TGG 0.287109 8.4:-60138812 MS.gene92414:CDS
TAACGAGTTTACATTCAATA+TGG 0.323889 8.4:+60138676 None:intergenic
ATACAGGTAGTGACCTTGTT+TGG 0.346831 8.4:-60139650 MS.gene92414:CDS
TCATTTCCTAAAACAATGAT+AGG 0.347087 8.4:-60139387 MS.gene92414:CDS
TCTCTTATTGTTTGGGTCCA+TGG 0.348492 8.4:-60139251 MS.gene92414:CDS
GGTCAAATATAGGTGTGATT+TGG 0.351752 8.4:+60139595 None:intergenic
CAAAGTTTAAATGTACCATT+TGG 0.354552 8.4:+60138934 None:intergenic
TGAGAGAATATTTGTAGAAA+TGG 0.355681 8.4:+60139769 None:intergenic
CCCATCAGCAACTTTAATAA+AGG 0.357410 8.4:+60138825 None:intergenic
TGCTATAATGTTGCGTATCA+TGG 0.359437 8.4:-60138913 MS.gene92414:CDS
TATGTCTCTTCCATCTCAAT+TGG 0.359626 8.4:-60139295 MS.gene92414:CDS
CCGACAAGCAAGGTATGTTT+TGG 0.369527 8.4:+60139553 None:intergenic
CCATCTCAAAGAGCCATCTT+TGG 0.370078 8.4:-60138781 MS.gene92414:CDS
TCAACTGTAAACTCTGATAA+AGG 0.374163 8.4:-60139732 MS.gene92414:CDS
GCCGCGGTGGTTTATGGTGA+TGG 0.392679 8.4:-60139192 MS.gene92414:CDS
TTGAGCCACGTTTCCAAAGA+TGG 0.399080 8.4:+60138768 None:intergenic
CCGCGGTGGTTTATGGTGAT+GGG 0.401200 8.4:-60139191 MS.gene92414:CDS
GATAATGAAGGGTCAAATAT+AGG 0.401499 8.4:+60139585 None:intergenic
AGAAATACGGGGACGTTTCA+TGG 0.401543 8.4:-60139354 MS.gene92414:CDS
CCAAAACATACCTTGCTTGT+CGG 0.403977 8.4:-60139553 MS.gene92414:CDS
TCAACGAGCAAACTCAATTT+CGG 0.420697 8.4:-60139219 MS.gene92414:CDS
AATCTATAATAAACATCATA+TGG 0.421746 8.4:+60139003 None:intergenic
TCTCAATTGGGTACATCAAT+TGG 0.426403 8.4:-60139282 MS.gene92414:CDS
CCAAAGATGGCTCTTTGAGA+TGG 0.441302 8.4:+60138781 None:intergenic
GTATTTGGCATTGTTGATAC+AGG 0.448885 8.4:-60139666 MS.gene92414:CDS
ACGTTTCATGGCCCAAGCTC+TGG 0.450877 8.4:-60139342 MS.gene92414:CDS
AGCTCTGGTATAGTTGGCCT+TGG 0.454633 8.4:-60139327 MS.gene92414:CDS
TGTAGCAGAATATATTAATC+TGG 0.456422 8.4:-60138971 MS.gene92414:CDS
TGGTAACTTGATGATAATGA+AGG 0.458982 8.4:+60139573 None:intergenic
GGCCCAAGCTCTGGTATAGT+TGG 0.464269 8.4:-60139333 MS.gene92414:CDS
ACTACCTCTTGTGATGTACT+AGG 0.468273 8.4:-60139510 MS.gene92414:CDS
AGGATGTGGATACAGAAATA+CGG 0.478251 8.4:-60139367 MS.gene92414:CDS
TATGGCTTGGTACAATCAAC+AGG 0.479709 8.4:+60138694 None:intergenic
CAAAGATGGCTCTTTGAGAT+GGG 0.480521 8.4:+60138782 None:intergenic
TGCCAAATACCTTAAATGGC+GGG 0.483575 8.4:+60139679 None:intergenic
ACCTTTATTAAAGTTGCTGA+TGG 0.490311 8.4:-60138826 MS.gene92414:CDS
GGTATAGTTGGCCTTGGAAG+CGG 0.493170 8.4:-60139321 MS.gene92414:CDS
ATGTCTCTTCCATCTCAATT+GGG 0.496511 8.4:-60139294 MS.gene92414:CDS
CGTGGCTCAACAGAACTTGT+TGG 0.505666 8.4:-60138755 MS.gene92414:CDS
AGTTTACATTCAATATGGCT+TGG 0.507657 8.4:+60138681 None:intergenic
AGGCCAACTATACCAGAGCT+TGG 0.510641 8.4:+60139330 None:intergenic
TCAGAGTTTACAGTTGATTG+AGG 0.512751 8.4:+60139738 None:intergenic
GGATGTGGATACAGAAATAC+GGG 0.513061 8.4:-60139366 MS.gene92414:CDS
GGCCAACTATACCAGAGCTT+GGG 0.516474 8.4:+60139331 None:intergenic
GAGACATAGGTCCGCTTCCA+AGG 0.518397 8.4:+60139310 None:intergenic
CTTTCTCATGCTCTAAACAA+TGG 0.518412 8.4:-60139906 MS.gene92414:CDS
GAATTTGGAGGACCTACATA+TGG 0.520740 8.4:-60139081 MS.gene92414:CDS
GAAATGGTTGACACGGTTGA+TGG 0.522721 8.4:+60139785 None:intergenic
CCTAAAACAATGATAGGATG+TGG 0.529275 8.4:-60139381 MS.gene92414:CDS
AGGAAATGAAACTGAGTAGC+CGG 0.538541 8.4:+60139401 None:intergenic
CAAATATAGGTGTGATTTGG+GGG 0.539813 8.4:+60139598 None:intergenic
TGAAACTGAGTAGCCGGTGG+TGG 0.544154 8.4:+60139407 None:intergenic
TCAAATATAGGTGTGATTTG+GGG 0.549910 8.4:+60139597 None:intergenic
TATACTTCTAAGTATATGGA+TGG 0.553688 8.4:-60139477 MS.gene92414:CDS
CCTTTATTAAAGTTGCTGAT+GGG 0.554774 8.4:-60138825 MS.gene92414:CDS
GATGGAACGACGCACAGCGT+TGG 0.559463 8.4:+60139803 None:intergenic
ACATTGGAAGCATTTAGTGT+AGG 0.561673 8.4:-60139117 MS.gene92414:CDS
GGTAACTTGATGATAATGAA+GGG 0.561789 8.4:+60139574 None:intergenic
GAGCATGTTGAGGATCCAAA+TGG 0.569777 8.4:-60138949 MS.gene92414:CDS
CGCGGTGGTTTATGGTGATG+GGG 0.573098 8.4:-60139190 MS.gene92414:CDS
GCCAAATACCTTAAATGGCG+GGG 0.573553 8.4:+60139680 None:intergenic
AATTGAGATGGAAGAGACAT+AGG 0.574625 8.4:+60139297 None:intergenic
CTCGTTGAGTTAGACAACCA+TGG 0.581026 8.4:+60139234 None:intergenic
GACAAATTAATAGAATTTGG+AGG 0.581912 8.4:-60139093 MS.gene92414:CDS
CAATTGGGTACATCAATTGG+TGG 0.588397 8.4:-60139279 MS.gene92414:CDS
GCTCAACAGAACTTGTTGGT+TGG 0.588410 8.4:-60138751 MS.gene92414:CDS
CTTACATTAGATTCCACCAC+CGG 0.589724 8.4:-60139420 MS.gene92414:CDS
TATCTCATGAGTTATTCGAT+TGG 0.591178 8.4:-60139705 MS.gene92414:CDS
TATTAATCTGGAGCATGTTG+AGG 0.595400 8.4:-60138959 MS.gene92414:CDS
ATTGATGTACCCAATTGAGA+TGG 0.596561 8.4:+60139285 None:intergenic
ATTTGTAGAAATGGTTGACA+CGG 0.603185 8.4:+60139778 None:intergenic
AAAACCTAGTACATCACAAG+AGG 0.605654 8.4:+60139506 None:intergenic
GCTCACATTGAAGCCAAACA+AGG 0.615577 8.4:+60139637 None:intergenic
CAATTTCGGAGATGCCGCGG+TGG 0.620480 8.4:-60139205 MS.gene92414:CDS
ATGCCAAATACCTTAAATGG+CGG 0.625151 8.4:+60139678 None:intergenic
AAGAGCCATCTTTGGAAACG+TGG 0.625504 8.4:-60138773 MS.gene92414:CDS
AAGTGGTTACTACTTGACAT+TGG 0.627228 8.4:-60139133 MS.gene92414:CDS
TGACATGTGTCCGACAAGCA+AGG 0.631262 8.4:+60139543 None:intergenic
TTTGGAGGACCTACATATGG+TGG 0.644320 8.4:-60139078 MS.gene92414:CDS
ACTCAATTTCGGAGATGCCG+CGG 0.645060 8.4:-60139208 MS.gene92414:CDS
TTCACAAGGATATCTTAGTG+TGG 0.648065 8.4:-60139448 MS.gene92414:CDS
ATGGATGGATCAATTTCACA+AGG 0.690637 8.4:-60139462 MS.gene92414:CDS
AAATGAAACTGAGTAGCCGG+TGG 0.704438 8.4:+60139404 None:intergenic
CCCATCACCATAAACCACCG+CGG 0.741460 8.4:+60139191 None:intergenic
GATGTGGATACAGAAATACG+GGG 0.856363 8.4:-60139365 MS.gene92414:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCTATAATAAACATCATA+TGG + chr8.4:60139656-60139675 None:intergenic 15.0%
!! AGAAATAATGAAACAAAAAA+TGG + chr8.4:60138730-60138749 None:intergenic 15.0%
!! TGAATATACTTCTAAGTATA+TGG - chr8.4:60139175-60139194 MS.gene92414:CDS 20.0%
! CAAAGTTTAAATGTACCATT+TGG + chr8.4:60139725-60139744 None:intergenic 25.0%
! GACAAATTAATAGAATTTGG+AGG - chr8.4:60139563-60139582 MS.gene92414:CDS 25.0%
! GGAAAATTCTCTTATTGTTT+GGG - chr8.4:60139398-60139417 MS.gene92414:CDS 25.0%
! GGAGACAAATTAATAGAATT+TGG - chr8.4:60139560-60139579 MS.gene92414:CDS 25.0%
! TATACTTCTAAGTATATGGA+TGG - chr8.4:60139179-60139198 MS.gene92414:CDS 25.0%
! TCATTTCCTAAAACAATGAT+AGG - chr8.4:60139269-60139288 MS.gene92414:CDS 25.0%
! TGAGAGAATATTTGTAGAAA+TGG + chr8.4:60138890-60138909 None:intergenic 25.0%
! TGGAAAATTCTCTTATTGTT+TGG - chr8.4:60139397-60139416 MS.gene92414:CDS 25.0%
! TGTAGCAGAATATATTAATC+TGG - chr8.4:60139685-60139704 MS.gene92414:CDS 25.0%
! TTAAAATGTGCAGTGATTAA+AGG + chr8.4:60139779-60139798 None:intergenic 25.0%
!! TTAATCACTGCACATTTTAA+AGG - chr8.4:60139779-60139798 MS.gene92414:CDS 25.0%
!!! GGTAACATTTTAATTGATTC+TGG - chr8.4:60139611-60139630 MS.gene92414:CDS 25.0%
ACAATGCCAAATACCTTAAA+TGG + chr8.4:60138984-60139003 None:intergenic 30.0%
ACATATGGTGGTAAAAATGA+GGG - chr8.4:60139590-60139609 MS.gene92414:CDS 30.0%
ACCTTTATTAAAGTTGCTGA+TGG - chr8.4:60139830-60139849 MS.gene92414:CDS 30.0%
ATTTGTAGAAATGGTTGACA+CGG + chr8.4:60138881-60138900 None:intergenic 30.0%
CCTTTATTAAAGTTGCTGAT+GGG - chr8.4:60139831-60139850 MS.gene92414:CDS 30.0%
GATAATGAAGGGTCAAATAT+AGG + chr8.4:60139074-60139093 None:intergenic 30.0%
GTCAAATATAGGTGTGATTT+GGG + chr8.4:60139063-60139082 None:intergenic 30.0%
TACATATGGTGGTAAAAATG+AGG - chr8.4:60139589-60139608 MS.gene92414:CDS 30.0%
TATCTCATGAGTTATTCGAT+TGG - chr8.4:60138951-60138970 MS.gene92414:CDS 30.0%
TCAAATATAGGTGTGATTTG+GGG + chr8.4:60139062-60139081 None:intergenic 30.0%
TCAACTGTAAACTCTGATAA+AGG - chr8.4:60138924-60138943 MS.gene92414:CDS 30.0%
! GGTAACTTGATGATAATGAA+GGG + chr8.4:60139085-60139104 None:intergenic 30.0%
! TGGTAACTTGATGATAATGA+AGG + chr8.4:60139086-60139105 None:intergenic 30.0%
!! TCATTTTTACCACCATATGT+AGG + chr8.4:60139590-60139609 None:intergenic 30.0%
!!! CTTTCGTATTTGTTTTGTGT+AGG + chr8.4:60138828-60138847 None:intergenic 30.0%
!!! TGTGCATACTTTTTCACTAT+AGG + chr8.4:60139503-60139522 None:intergenic 30.0%
AAAACCTAGTACATCACAAG+AGG + chr8.4:60139153-60139172 None:intergenic 35.0%
AAGTGGTTACTACTTGACAT+TGG - chr8.4:60139523-60139542 MS.gene92414:CDS 35.0%
AATTGAGATGGAAGAGACAT+AGG + chr8.4:60139362-60139381 None:intergenic 35.0%
AGGATGTGGATACAGAAATA+CGG - chr8.4:60139289-60139308 MS.gene92414:CDS 35.0%
ATGCCAAATACCTTAAATGG+CGG + chr8.4:60138981-60139000 None:intergenic 35.0%
ATGGATGGATCAATTTCACA+AGG - chr8.4:60139194-60139213 MS.gene92414:CDS 35.0%
ATGTCTCTTCCATCTCAATT+GGG - chr8.4:60139362-60139381 MS.gene92414:CDS 35.0%
ATTGATGTACCCAATTGAGA+TGG + chr8.4:60139374-60139393 None:intergenic 35.0%
CCCATCAGCAACTTTAATAA+AGG + chr8.4:60139834-60139853 None:intergenic 35.0%
CCTAAAACAATGATAGGATG+TGG - chr8.4:60139275-60139294 MS.gene92414:CDS 35.0%
GGTCAAATATAGGTGTGATT+TGG + chr8.4:60139064-60139083 None:intergenic 35.0%
GTATTTGGCATTGTTGATAC+AGG - chr8.4:60138990-60139009 MS.gene92414:CDS 35.0%
TATGTCTCTTCCATCTCAAT+TGG - chr8.4:60139361-60139380 MS.gene92414:CDS 35.0%
TCAACGAGCAAACTCAATTT+CGG - chr8.4:60139437-60139456 MS.gene92414:CDS 35.0%
TCAGAGTTTACAGTTGATTG+AGG + chr8.4:60138921-60138940 None:intergenic 35.0%
TCTCAATTGGGTACATCAAT+TGG - chr8.4:60139374-60139393 MS.gene92414:CDS 35.0%
TGCTATAATGTTGCGTATCA+TGG - chr8.4:60139743-60139762 MS.gene92414:CDS 35.0%
TTCACAAGGATATCTTAGTG+TGG - chr8.4:60139208-60139227 MS.gene92414:CDS 35.0%
! CAAATATAGGTGTGATTTGG+GGG + chr8.4:60139061-60139080 None:intergenic 35.0%
! CCACATCCTATCATTGTTTT+AGG + chr8.4:60139278-60139297 None:intergenic 35.0%
! CTTTCTCATGCTCTAAACAA+TGG - chr8.4:60138750-60138769 MS.gene92414:CDS 35.0%
! GTGAAAAAGTATGCACAAAG+TGG - chr8.4:60139506-60139525 MS.gene92414:CDS 35.0%
! TATTAATCTGGAGCATGTTG+AGG - chr8.4:60139697-60139716 MS.gene92414:CDS 35.0%
!! ACATTGGAAGCATTTAGTGT+AGG - chr8.4:60139539-60139558 MS.gene92414:CDS 35.0%
!!! CGTATTTGTTTTGTGTAGGT+TGG + chr8.4:60138824-60138843 None:intergenic 35.0%
!!! TTTTGTGTAGGTTGGTAGAA+TGG + chr8.4:60138816-60138835 None:intergenic 35.0%
ACTACCTCTTGTGATGTACT+AGG - chr8.4:60139146-60139165 MS.gene92414:CDS 40.0%
AGGAAATGAAACTGAGTAGC+CGG + chr8.4:60139258-60139277 None:intergenic 40.0%
ATACAGGTAGTGACCTTGTT+TGG - chr8.4:60139006-60139025 MS.gene92414:CDS 40.0%
CAATTGGGTACATCAATTGG+TGG - chr8.4:60139377-60139396 MS.gene92414:CDS 40.0%
CCAAAACATACCTTGCTTGT+CGG - chr8.4:60139103-60139122 MS.gene92414:CDS 40.0%
CTTACATTAGATTCCACCAC+CGG - chr8.4:60139236-60139255 MS.gene92414:CDS 40.0%
GAATTTGGAGGACCTACATA+TGG - chr8.4:60139575-60139594 MS.gene92414:CDS 40.0%
GATGTGGATACAGAAATACG+GGG - chr8.4:60139291-60139310 MS.gene92414:CDS 40.0%
GGATGTGGATACAGAAATAC+GGG - chr8.4:60139290-60139309 MS.gene92414:CDS 40.0%
TCTCTTATTGTTTGGGTCCA+TGG - chr8.4:60139405-60139424 MS.gene92414:CDS 40.0%
TGCCAAATACCTTAAATGGC+GGG + chr8.4:60138980-60138999 None:intergenic 40.0%
!! CAAAGATGGCTCTTTGAGAT+GGG + chr8.4:60139877-60139896 None:intergenic 40.0%
!! GTGATTTTGATGAGTCACGA+TGG + chr8.4:60138794-60138813 None:intergenic 40.0%
!! TATGGCTTGGTACAATCAAC+AGG + chr8.4:60139965-60139984 None:intergenic 40.0%
AAATGAAACTGAGTAGCCGG+TGG + chr8.4:60139255-60139274 None:intergenic 45.0%
AGAAATACGGGGACGTTTCA+TGG - chr8.4:60139302-60139321 MS.gene92414:CDS 45.0%
CCATCTCAAAGAGCCATCTT+TGG - chr8.4:60139875-60139894 MS.gene92414:CDS 45.0%
CTCGTTGAGTTAGACAACCA+TGG + chr8.4:60139425-60139444 None:intergenic 45.0%
GAAATGGTTGACACGGTTGA+TGG + chr8.4:60138874-60138893 None:intergenic 45.0%
GAGCATGTTGAGGATCCAAA+TGG - chr8.4:60139707-60139726 MS.gene92414:CDS 45.0%
GCCAAATACCTTAAATGGCG+GGG + chr8.4:60138979-60138998 None:intergenic 45.0%
GCTCACATTGAAGCCAAACA+AGG + chr8.4:60139022-60139041 None:intergenic 45.0%
TGGGGGTAACATTGTTCACA+AGG + chr8.4:60139044-60139063 None:intergenic 45.0%
TTGAGCCACGTTTCCAAAGA+TGG + chr8.4:60139891-60139910 None:intergenic 45.0%
TTTGGAGGACCTACATATGG+TGG - chr8.4:60139578-60139597 MS.gene92414:CDS 45.0%
! AAGAGCCATCTTTGGAAACG+TGG - chr8.4:60139883-60139902 MS.gene92414:CDS 45.0%
! ACCCCGCCATTTAAGGTATT+TGG - chr8.4:60138975-60138994 MS.gene92414:CDS 45.0%
! CCAAAGATGGCTCTTTGAGA+TGG + chr8.4:60139878-60139897 None:intergenic 45.0%
! CCGACAAGCAAGGTATGTTT+TGG + chr8.4:60139106-60139125 None:intergenic 45.0%
!! GCTCAACAGAACTTGTTGGT+TGG - chr8.4:60139905-60139924 MS.gene92414:CDS 45.0%
ACTCAATTTCGGAGATGCCG+CGG - chr8.4:60139448-60139467 MS.gene92414:CDS 50.0%
AGGCCAACTATACCAGAGCT+TGG + chr8.4:60139329-60139348 None:intergenic 50.0%
CGTGGCTCAACAGAACTTGT+TGG - chr8.4:60139901-60139920 MS.gene92414:CDS 50.0%
GATTGGTACCCCGCCATTTA+AGG - chr8.4:60138968-60138987 MS.gene92414:CDS 50.0%
GGCCAACTATACCAGAGCTT+GGG + chr8.4:60139328-60139347 None:intergenic 50.0%
TGACATGTGTCCGACAAGCA+AGG + chr8.4:60139116-60139135 None:intergenic 50.0%
TGCTGATGGGATTGCTTGCT+TGG - chr8.4:60139844-60139863 MS.gene92414:CDS 50.0%
! AGCTCTGGTATAGTTGGCCT+TGG - chr8.4:60139329-60139348 MS.gene92414:CDS 50.0%
!! GGTATAGTTGGCCTTGGAAG+CGG - chr8.4:60139335-60139354 MS.gene92414:CDS 50.0%
ACGTTTCATGGCCCAAGCTC+TGG - chr8.4:60139314-60139333 MS.gene92414:CDS 55.0%
CCCATCACCATAAACCACCG+CGG + chr8.4:60139468-60139487 None:intergenic 55.0%
CCGCGGTGGTTTATGGTGAT+GGG - chr8.4:60139465-60139484 MS.gene92414:CDS 55.0%
GAGACATAGGTCCGCTTCCA+AGG + chr8.4:60139349-60139368 None:intergenic 55.0%
TGAAACTGAGTAGCCGGTGG+TGG + chr8.4:60139252-60139271 None:intergenic 55.0%
! CGCGGTGGTTTATGGTGATG+GGG - chr8.4:60139466-60139485 MS.gene92414:CDS 55.0%
! GGCCCAAGCTCTGGTATAGT+TGG - chr8.4:60139323-60139342 MS.gene92414:CDS 55.0%
CAATTTCGGAGATGCCGCGG+TGG - chr8.4:60139451-60139470 MS.gene92414:CDS 60.0%
GATGGAACGACGCACAGCGT+TGG + chr8.4:60138856-60138875 None:intergenic 60.0%
GCCGCGGTGGTTTATGGTGA+TGG - chr8.4:60139464-60139483 MS.gene92414:CDS 60.0%
GGAGATGCCGCGGTGGTTTA+TGG - chr8.4:60139458-60139477 MS.gene92414:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 60138690 60139988 60138690 ID=MS.gene92414
chr8.4 mRNA 60138690 60139988 60138690 ID=MS.gene92414.t1;Parent=MS.gene92414
chr8.4 exon 60138690 60139988 60138690 ID=MS.gene92414.t1.exon1;Parent=MS.gene92414.t1
chr8.4 CDS 60138690 60139988 60138690 ID=cds.MS.gene92414.t1;Parent=MS.gene92414.t1
Gene Sequence

>MS.gene92414

ATGAGTGCACATTCATTTCTTACACTTCTTTTCTTTACCATTTTTTGTTTCATTATTTCTCTTTCTCATGCTCTAAACAATGGTTTCACTCTTGAACTCATCCATCGTGACTCATCAAAATCACCATTCTACCAACCTACACAAAACAAATACGAAAGTGTTGCCAACGCTGTGCGTCGTTCCATCAACCGTGTCAACCATTTCTACAAATATTCTCTCACTAGTACACCTCAATCAACTGTAAACTCTGATAAAGGTGAGTATCTCATGAGTTATTCGATTGGTACCCCGCCATTTAAGGTATTTGGCATTGTTGATACAGGTAGTGACCTTGTTTGGCTTCAATGTGAGCCTTGTGAACAATGTTACCCCCAAATCACACCTATATTTGACCCTTCATTATCATCAAGTTACCAAAACATACCTTGCTTGTCGGACACATGTCAATCAATGAGAACTACCTCTTGTGATGTACTAGGTTTTTGTGAATATACTTCTAAGTATATGGATGGATCAATTTCACAAGGATATCTTAGTGTGGAGACTCTTACATTAGATTCCACCACCGGCTACTCAGTTTCATTTCCTAAAACAATGATAGGATGTGGATACAGAAATACGGGGACGTTTCATGGCCCAAGCTCTGGTATAGTTGGCCTTGGAAGCGGACCTATGTCTCTTCCATCTCAATTGGGTACATCAATTGGTGGAAAATTCTCTTATTGTTTGGGTCCATGGTTGTCTAACTCAACGAGCAAACTCAATTTCGGAGATGCCGCGGTGGTTTATGGTGATGGGGCTATGACAACTCCTATAGTGAAAAAGTATGCACAAAGTGGTTACTACTTGACATTGGAAGCATTTAGTGTAGGAGACAAATTAATAGAATTTGGAGGACCTACATATGGTGGTAAAAATGAGGGTAACATTTTAATTGATTCTGGTACAACATTTACATTTTTGCCATATGATGTTTATTATAGATTTGAATCAGCTGTAGCAGAATATATTAATCTGGAGCATGTTGAGGATCCAAATGGTACATTTAAACTTTGCTATAATGTTGCGTATCATGGATTTGAAGCTCCTTTAATCACTGCACATTTTAAAGGTGCAGATATTAAATTGTATTCTATTAGTACCTTTATTAAAGTTGCTGATGGGATTGCTTGCTTGGCTTTTATCCCATCTCAAAGAGCCATCTTTGGAAACGTGGCTCAACAGAACTTGTTGGTTGGTTATAATCTTGTTCAAAATACTGTTACATTTAAGCCTGTTGATTGTACCAAGCCATATTGA
Protein sequence

>MS.gene92414.t1

MSAHSFLTLLFFTIFCFIISLSHALNNGFTLELIHRDSSKSPFYQPTQNKYESVANAVRRSINRVNHFYKYSLTSTPQSTVNSDKGEYLMSYSIGTPPFKVFGIVDTGSDLVWLQCEPCEQCYPQITPIFDPSLSSSYQNIPCLSDTCQSMRTTSCDVLGFCEYTSKYMDGSISQGYLSVETLTLDSTTGYSVSFPKTMIGCGYRNTGTFHGPSSGIVGLGSGPMSLPSQLGTSIGGKFSYCLGPWLSNSTSKLNFGDAAVVYGDGAMTTPIVKKYAQSGYYLTLEAFSVGDKLIEFGGPTYGGKNEGNILIDSGTTFTFLPYDVYYRFESAVAEYINLEHVEDPNGTFKLCYNVAYHGFEAPLITAHFKGADIKLYSISTFIKVADGIACLAFIPSQRAIFGNVAQQNLLVGYNLVQNTVTFKPVDCTKPY