AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92438

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92438.t1 MTR_8g038280 85.780 436 56 2 1 430 1 436 0.0 756
MS.gene92438.t1 MTR_5g080890 73.059 438 106 5 1 430 1 434 0.0 644
MS.gene92438.t1 MTR_5g080960 72.603 438 108 5 1 430 1 434 0.0 641
MS.gene92438.t1 MTR_8g096990 71.101 436 116 3 1 430 1 432 0.0 629
MS.gene92438.t1 MTR_5g044770 68.966 435 126 3 1 430 1 431 0.0 600
MS.gene92438.t1 MTR_8g038250 68.778 442 100 5 1 430 1 416 0.0 597
MS.gene92438.t1 MTR_6g090290 65.138 436 145 3 1 429 1 436 0.0 541
MS.gene92438.t1 MTR_8g038310 60.092 436 144 6 1 429 1 413 0.0 513
MS.gene92438.t1 MTR_4g109760 62.844 436 119 7 1 430 1 399 2.23e-168 478
MS.gene92438.t1 MTR_3g028120 50.355 423 192 7 22 430 21 439 2.72e-142 413
MS.gene92438.t1 MTR_5g038360 49.771 436 197 9 9 429 8 436 2.23e-135 396
MS.gene92438.t1 MTR_2g067830 52.927 410 177 8 32 429 1 406 2.52e-133 389
MS.gene92438.t1 MTR_6g088760 51.220 410 181 9 1 396 1 405 1.49e-130 386
MS.gene92438.t1 MTR_0024s0040 49.640 417 186 9 30 429 1 410 7.10e-127 373
MS.gene92438.t1 MTR_5g044570 47.630 443 186 11 1 429 1 411 1.03e-126 373
MS.gene92438.t1 MTR_2g105300 49.877 407 166 9 32 426 1 381 2.43e-119 353
MS.gene92438.t1 MTR_0036s0070 41.232 422 224 10 19 429 18 426 2.22e-101 308
MS.gene92438.t1 MTR_0036s0050 41.723 441 227 13 9 429 5 435 3.35e-99 303
MS.gene92438.t1 MTR_0036s0110 39.956 453 237 13 1 429 1 442 3.76e-95 293
MS.gene92438.t1 MTR_5g008070 41.787 414 212 10 27 428 29 425 1.78e-91 283
MS.gene92438.t1 MTR_2g059460 42.386 394 196 13 52 430 30 407 3.36e-86 269
MS.gene92438.t1 MTR_2g059490 38.600 443 222 12 3 430 4 411 4.50e-85 267
MS.gene92438.t1 MTR_5g008110 38.161 435 217 12 7 429 21 415 2.21e-84 265
MS.gene92438.t1 MTR_5g008150 40.365 384 209 9 59 429 36 412 7.77e-84 263
MS.gene92438.t1 MTR_3g097150 35.965 456 252 14 2 430 7 449 1.58e-77 248
MS.gene92438.t1 MTR_1g060750 35.980 403 217 12 40 429 2 376 5.17e-68 221
MS.gene92438.t1 MTR_7g117430 33.871 434 235 16 24 427 73 484 9.05e-57 194
MS.gene92438.t1 MTR_4g045857 37.107 318 154 9 3 309 6 288 7.31e-55 184
MS.gene92438.t1 MTR_5g008090 39.000 300 165 8 142 429 21 314 7.87e-55 184
MS.gene92438.t1 MTR_1g115210 32.227 422 245 15 30 427 82 486 7.39e-53 184
MS.gene92438.t1 MTR_1g039270 31.828 443 240 20 22 427 52 469 1.38e-48 172
MS.gene92438.t1 MTR_1g111350 33.894 357 203 12 85 427 130 467 3.67e-46 166
MS.gene92438.t1 MTR_2g028190 31.155 459 271 16 5 427 2 451 1.23e-45 164
MS.gene92438.t1 MTR_6g085010 33.616 354 205 12 86 427 156 491 2.29e-44 161
MS.gene92438.t1 MTR_8g103880 29.235 366 233 11 62 415 92 443 5.20e-42 154
MS.gene92438.t1 MTR_3g046300 30.749 387 240 12 68 430 79 461 5.96e-41 151
MS.gene92438.t1 MTR_6g088765 39.419 241 110 5 162 399 5 212 3.07e-40 144
MS.gene92438.t1 MTR_5g091130 29.070 430 269 14 24 428 136 554 1.18e-38 147
MS.gene92438.t1 MTR_4g007740 32.692 364 205 13 87 428 133 478 2.94e-38 144
MS.gene92438.t1 MTR_3g053710 31.405 363 217 12 85 427 116 466 1.41e-35 137
MS.gene92438.t1 MTR_2g070430 27.234 470 285 19 2 429 3 457 4.72e-32 127
MS.gene92438.t1 MTR_3g065160 28.108 370 223 15 88 430 76 429 1.55e-31 125
MS.gene92438.t1 MTR_2g055840 28.421 380 231 13 78 427 5 373 4.75e-29 117
MS.gene92438.t1 MTR_3g068000 27.895 380 219 15 89 427 76 441 2.38e-28 116
MS.gene92438.t1 MTR_7g105850 28.601 479 266 21 9 429 7 467 7.30e-28 115
MS.gene92438.t1 MTR_2g063110 27.723 404 219 17 85 427 117 508 2.78e-27 114
MS.gene92438.t1 MTR_5g077850 27.441 379 218 14 88 430 83 440 4.37e-26 110
MS.gene92438.t1 MTR_3g053980 30.400 375 224 14 73 427 128 485 3.69e-25 107
MS.gene92438.t1 MTR_7g062360 26.720 378 224 14 89 427 62 425 1.71e-24 105
MS.gene92438.t1 MTR_8g094590 26.121 379 224 17 85 430 72 427 3.51e-24 104
MS.gene92438.t1 MTR_3g096930 25.789 380 223 17 85 430 83 437 5.37e-23 100
MS.gene92438.t1 MTR_1g061940 26.566 463 274 22 8 428 7 445 6.75e-23 101
MS.gene92438.t1 MTR_8g094590 30.502 259 147 10 85 331 72 309 8.46e-23 98.6
MS.gene92438.t1 MTR_8g094590 28.667 300 175 12 85 368 72 348 1.25e-22 99.8
MS.gene92438.t1 MTR_1g078240 26.440 382 224 15 85 428 68 430 1.31e-22 100
MS.gene92438.t1 MTR_4g095270 26.810 373 223 17 84 430 78 426 3.06e-22 99.8
MS.gene92438.t1 MTR_4g095270 26.810 373 223 17 84 430 78 426 4.19e-22 99.4
MS.gene92438.t1 MTR_1g078240 26.632 383 223 16 85 428 68 431 2.05e-21 96.7
MS.gene92438.t1 MTR_7g021510 26.343 391 229 19 75 428 66 434 2.76e-21 96.3
MS.gene92438.t1 MTR_7g080230 24.099 444 269 18 30 428 11 431 3.06e-21 96.3
MS.gene92438.t1 MTR_6g084710 25.526 380 245 12 76 427 181 550 3.47e-21 96.3
MS.gene92438.t1 MTR_5g016260 24.119 369 238 14 84 430 86 434 3.76e-21 96.3
MS.gene92438.t1 MTR_7g102750 27.660 376 226 17 85 428 80 441 3.79e-21 95.9
MS.gene92438.t1 MTR_5g016260 24.119 369 238 14 84 430 86 434 4.91e-21 96.3
MS.gene92438.t1 MTR_0945s0010 40.945 127 69 4 308 430 1 125 5.73e-21 89.7
MS.gene92438.t1 MTR_2g103340 41.600 125 28 2 21 145 34 113 7.80e-21 90.1
MS.gene92438.t1 MTR_3g014290 27.701 361 218 14 87 428 81 417 7.83e-21 94.4
MS.gene92438.t1 MTR_2g084550 29.101 378 219 18 85 428 76 438 1.09e-20 94.7
MS.gene92438.t1 MTR_1g037630 26.531 441 252 19 24 428 25 429 1.28e-20 94.0
MS.gene92438.t1 MTR_8g039540 26.522 460 270 19 6 428 6 434 2.21e-20 93.2
MS.gene92438.t1 MTR_6g084710 26.923 286 189 9 76 351 181 456 4.41e-20 92.8
MS.gene92438.t1 MTR_4g056420 27.321 377 226 18 85 428 76 437 6.75e-19 89.0
MS.gene92438.t1 MTR_3g087780 24.945 453 264 24 28 430 27 453 8.08e-19 89.0
MS.gene92438.t1 MTR_5g022880 26.712 438 268 18 27 427 40 461 1.01e-18 88.6
MS.gene92438.t1 MTR_4g008110 42.735 117 43 2 158 274 45 137 1.98e-18 81.6
MS.gene92438.t1 MTR_3g006820 25.862 406 240 19 64 430 32 415 4.49e-18 86.3
MS.gene92438.t1 MTR_1g078240 27.532 316 180 13 85 370 68 364 5.21e-18 85.5
MS.gene92438.t1 MTR_1g029810 25.397 378 229 17 85 430 56 412 6.45e-18 85.9
MS.gene92438.t1 MTR_1g029810 25.132 378 230 17 85 430 56 412 8.17e-18 85.5
MS.gene92438.t1 MTR_8g075090 27.733 375 213 18 87 429 113 461 1.07e-17 85.5
MS.gene92438.t1 MTR_7g102750 27.907 344 205 15 85 398 80 410 4.55e-17 83.2
MS.gene92438.t1 MTR_8g075090 27.078 373 214 18 87 427 113 459 9.75e-17 82.4
MS.gene92438.t1 MTR_4g100990 26.456 412 224 17 86 430 74 473 4.32e-16 80.5
MS.gene92438.t1 MTR_5g077920 25.401 374 232 13 88 429 70 428 9.74e-16 79.3
MS.gene92438.t1 MTR_4g023650 25.130 386 228 19 84 430 59 422 1.54e-15 78.6
MS.gene92438.t1 MTR_5g012490 24.900 502 275 26 1 430 1 472 3.05e-14 74.7
MS.gene92438.t1 MTR_8g075090 27.003 337 196 16 87 395 113 427 3.80e-14 74.3
MS.gene92438.t1 MTR_4g023630 23.907 389 229 18 84 430 59 422 9.53e-14 73.2
MS.gene92438.t1 MTR_3g087780 26.543 324 195 18 129 430 9 311 1.31e-12 69.3
MS.gene92438.t1 MTR_3g087780 26.543 324 195 18 129 430 45 347 2.14e-12 68.9
MS.gene92438.t1 MTR_2g037710 29.187 209 119 8 227 427 18 205 2.59e-12 66.2
MS.gene92438.t1 MTR_7g080230 23.810 357 211 16 110 428 28 361 2.71e-12 68.6
MS.gene92438.t1 MTR_3g100500 26.558 369 218 19 85 427 96 437 3.33e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92438.t1 AT1G64830 49.034 414 198 6 25 430 23 431 3.28e-130 382
MS.gene92438.t1 AT5G33340 48.916 415 198 7 25 430 28 437 6.36e-119 354
MS.gene92438.t1 AT2G35615 42.760 442 222 8 14 428 7 444 1.25e-104 318
MS.gene92438.t1 AT1G31450 42.316 449 231 10 1 428 1 442 1.37e-98 302
MS.gene92438.t1 AT2G28010 37.327 434 210 13 9 430 11 394 4.87e-74 237
MS.gene92438.t1 AT2G03200 37.788 434 228 13 25 430 42 461 8.24e-74 238
MS.gene92438.t1 AT2G28040 38.776 441 196 20 6 430 11 393 7.73e-73 234
MS.gene92438.t1 AT2G28040 39.667 421 183 19 26 430 19 384 3.35e-71 229
MS.gene92438.t1 AT2G28220 34.899 447 227 14 1 430 1 400 2.82e-68 222
MS.gene92438.t1 AT2G28030 40.489 368 179 13 76 430 50 390 2.83e-65 214
MS.gene92438.t1 AT2G28225 34.004 447 231 15 1 430 1 400 3.00e-62 207
MS.gene92438.t1 AT1G01300 36.785 367 188 15 85 428 140 485 2.34e-53 185
MS.gene92438.t1 AT3G18490 31.066 441 264 15 7 427 80 500 1.82e-52 183
MS.gene92438.t1 AT4G30030 33.735 415 226 18 35 427 32 419 4.51e-52 181
MS.gene92438.t1 AT3G61820 34.937 395 216 16 55 427 108 483 5.52e-52 182
MS.gene92438.t1 AT2G23945 28.233 464 269 17 9 430 10 451 8.02e-49 172
MS.gene92438.t1 AT1G25510 34.916 358 198 14 85 427 146 483 6.76e-48 171
MS.gene92438.t1 AT3G20015 30.134 448 245 18 20 427 51 470 6.98e-47 168
MS.gene92438.t1 AT5G10760 34.293 382 222 15 57 427 101 464 3.44e-44 160
MS.gene92438.t1 AT4G30040 30.575 435 264 17 9 422 10 427 8.52e-42 153
MS.gene92438.t1 AT5G10770 34.341 364 200 15 85 428 130 474 1.54e-40 150
MS.gene92438.t1 AT3G12700 28.635 447 289 14 3 428 24 461 2.09e-38 144
MS.gene92438.t1 AT2G42980 31.649 376 221 15 85 430 158 527 3.55e-38 145
MS.gene92438.t1 AT3G59080 31.283 374 223 15 85 430 168 535 2.82e-37 142
MS.gene92438.t1 AT3G25700 28.372 430 250 18 39 428 39 450 6.27e-37 140
MS.gene92438.t1 AT1G79720 32.880 368 200 16 87 427 135 482 4.97e-36 138
MS.gene92438.t1 AT5G37540 27.733 375 219 15 88 427 81 438 2.23e-31 125
MS.gene92438.t1 AT5G36260 28.421 380 215 16 85 428 76 434 1.20e-30 123
MS.gene92438.t1 AT1G66180 26.415 371 231 15 88 430 73 429 6.63e-30 120
MS.gene92438.t1 AT2G39710 27.078 373 219 13 89 422 67 425 5.83e-29 118
MS.gene92438.t1 AT3G54400 29.695 394 226 15 62 428 56 425 8.06e-29 117
MS.gene92438.t1 AT3G59080 28.997 369 202 14 85 430 168 499 3.02e-28 117
MS.gene92438.t1 AT5G02190 27.778 378 210 19 96 427 82 442 4.16e-26 110
MS.gene92438.t1 AT4G12920 26.371 383 240 16 61 427 31 387 7.52e-24 102
MS.gene92438.t1 AT3G02740 25.806 372 234 12 85 428 83 440 2.18e-23 102
MS.gene92438.t1 AT4G33490 24.403 377 234 13 85 430 58 414 2.43e-23 102
MS.gene92438.t1 AT3G02740 25.946 370 232 12 87 428 10 365 5.89e-23 100
MS.gene92438.t1 AT1G65240 24.728 368 241 11 85 428 72 427 2.01e-22 99.8
MS.gene92438.t1 AT3G50050 25.269 372 231 13 84 430 90 439 5.86e-22 99.0
MS.gene92438.t1 AT5G45120 28.501 407 219 19 84 427 81 478 6.65e-22 98.2
MS.gene92438.t1 AT1G09750 26.598 391 238 17 67 427 77 448 2.44e-21 96.3
MS.gene92438.t1 AT2G36670 27.467 375 227 17 85 428 98 458 2.59e-21 96.7
MS.gene92438.t1 AT2G36670 27.346 373 226 17 87 428 105 463 5.93e-21 95.5
MS.gene92438.t1 AT2G36670 27.346 373 226 17 87 428 34 392 6.12e-21 95.1
MS.gene92438.t1 AT2G36670 27.346 373 226 17 87 428 34 392 6.12e-21 95.1
MS.gene92438.t1 AT1G05840 25.858 379 229 15 85 428 78 439 7.78e-21 95.1
MS.gene92438.t1 AT5G43100 25.995 377 220 18 84 430 73 420 1.07e-20 95.1
MS.gene92438.t1 AT5G43100 25.806 372 227 17 84 430 73 420 1.32e-20 94.4
MS.gene92438.t1 AT4G33490 26.510 298 185 10 85 368 55 332 2.08e-19 90.1
MS.gene92438.t1 AT3G51350 26.067 445 271 18 23 427 26 452 3.79e-19 90.1
MS.gene92438.t1 AT5G22850 27.660 376 227 17 85 428 79 441 1.00e-18 88.6
MS.gene92438.t1 AT1G08210 26.933 375 228 17 85 428 130 489 2.24e-18 87.8
MS.gene92438.t1 AT1G08210 26.933 375 228 17 85 428 116 475 2.96e-18 87.4
MS.gene92438.t1 AT1G08210 26.933 375 228 17 85 428 82 441 3.45e-18 87.0
MS.gene92438.t1 AT1G49050 22.365 389 231 17 82 429 39 397 4.82e-18 86.3
MS.gene92438.t1 AT1G49050 22.879 389 239 16 84 429 200 570 7.97e-18 86.3
MS.gene92438.t1 AT3G52500 28.030 396 218 19 85 428 88 468 9.01e-18 85.5
MS.gene92438.t1 AT2G17760 25.587 426 243 22 45 427 55 449 2.29e-17 84.7
MS.gene92438.t1 AT3G25700 28.249 177 106 6 39 202 39 207 2.73e-15 77.4
MS.gene92438.t1 AT3G42550 23.889 360 209 14 87 428 78 390 3.13e-15 77.8
MS.gene92438.t1 AT1G08210 25.373 402 227 18 85 428 82 468 7.62e-15 77.0

Find 93 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGAATATTCTACTTATTA+TGG 0.123621 8.4:-59800683 MS.gene92438:CDS
TGGCGTCATCTTTGGAAATT+TGG 0.135308 8.4:-59799973 MS.gene92438:CDS
GGTACAATCACTAGGTTTAA+AGG 0.207000 8.4:+59799902 None:intergenic
ACGACGAGCAAGCTCAATTT+TGG 0.216250 8.4:-59800410 MS.gene92438:CDS
GTCAAATAGTAAAAGGTTAT+TGG 0.216379 8.4:+59800329 None:intergenic
ACTCCACCATTTAAGTTATA+TGG 0.256368 8.4:-59800881 MS.gene92438:CDS
TCTCAAATTGGCGTCATCTT+TGG 0.276223 8.4:-59799981 MS.gene92438:CDS
GCAATACCATATAACTTAAA+TGG 0.279077 8.4:+59800875 None:intergenic
CTACAAAAGTCATCACATTT+TGG 0.283880 8.4:+59799881 None:intergenic
GGTAACATCATTATTGATTC+AGG 0.290403 8.4:-59800248 MS.gene92438:CDS
ACTGGAACAAATGTGCTAAT+AGG 0.295748 8.4:+59800038 None:intergenic
ACAAAGTTTCCCTTGCAAAT+AGG 0.300973 8.4:-59800961 MS.gene92438:CDS
GTGAATATTCTACTTATTAT+GGG 0.316224 8.4:-59800682 MS.gene92438:CDS
TTCCAATGTCAAATAGTAAA+AGG 0.325411 8.4:+59800322 None:intergenic
GGAAATAAAAGAGTAGAAAT+TGG 0.326779 8.4:-59800287 MS.gene92438:CDS
GGAGATGCGGCTGTGGTATC+TGG 0.329146 8.4:-59800389 MS.gene92438:CDS
ATGAGATACTCGCCGTCATC+AGG 0.341144 8.4:+59800920 None:intergenic
AACAAATGGAAAATAATAGA+AGG 0.359580 8.4:+59801140 None:intergenic
TTAAAATGTGCGGTGATTAG+AGG 0.364290 8.4:+59800083 None:intergenic
ACAGATTGACATAGCTTTGA+TGG 0.368927 8.4:+59800737 None:intergenic
TCAAATAGTAAAAGGTTATT+GGG 0.377983 8.4:+59800330 None:intergenic
TGCTGATGGTGTTAATTGCT+TGG 0.384661 8.4:-59800015 MS.gene92438:CDS
CTGGAACAAATGTGCTAATA+GGG 0.384780 8.4:+59800039 None:intergenic
GAAATCTGTTGTGGTAGATT+TGG 0.386651 8.4:-59800183 MS.gene92438:CDS
TCACATTCAGAGGGAGATCT+TGG 0.389279 8.4:-59800653 MS.gene92438:CDS
TCTAATATTGTGATAGGATG+TGG 0.390115 8.4:-59800581 MS.gene92438:CDS
ATACCGGTAGTGATATTGTT+TGG 0.392593 8.4:-59800850 MS.gene92438:CDS
GTGCGGCATTGACAAAATAT+TGG 0.399741 8.4:+59801014 None:intergenic
GGAAACTTTGTAGTAATGAT+TGG 0.403713 8.4:+59800973 None:intergenic
GGCATAAACTCTGGCATAGT+TGG 0.405114 8.4:-59800533 MS.gene92438:CDS
AACTCTGGCATAGTTGGCCT+TGG 0.407395 8.4:-59800527 MS.gene92438:CDS
CGGCTGTGGTATCTGGTGAC+GGG 0.412612 8.4:-59800382 MS.gene92438:CDS
GATTAAACATAGGACTGGTT+TGG 0.417379 8.4:+59800795 None:intergenic
GGCATAGTTGGCCTTGGAGG+TGG 0.421810 8.4:-59800521 MS.gene92438:CDS
TTTGGCTCAGCAAAATTTGT+TGG 0.424007 8.4:-59799955 MS.gene92438:CDS
AAAAGAAATAATGAAACAAA+TGG 0.430540 8.4:+59801126 None:intergenic
TGGTGGAGTACCAACTGAAT+AGG 0.444684 8.4:+59800895 None:intergenic
GTAATGATTGGCACGGTTGA+TGG 0.457739 8.4:+59800985 None:intergenic
TCTGGCATAGTTGGCCTTGG+AGG 0.460224 8.4:-59800524 MS.gene92438:CDS
AGAGAATTTACCACCAATTG+AGG 0.461430 8.4:+59800478 None:intergenic
GCAAAGGTCATATGTTTGAC+TGG 0.465989 8.4:+59800136 None:intergenic
CAATTAACACCATCAGCAAC+TGG 0.467780 8.4:+59800020 None:intergenic
AACAATATTATGTCTTATGA+AGG 0.482827 8.4:-59800554 MS.gene92438:CDS
TGGTGTTGATACTCTTACAT+TGG 0.484515 8.4:-59800633 MS.gene92438:CDS
GCGGCTGTGGTATCTGGTGA+CGG 0.489652 8.4:-59800383 MS.gene92438:CDS
ATGATGTTACCCTCATTTGA+AGG 0.493808 8.4:+59800260 None:intergenic
TTTATCACATCCTCAATTGG+TGG 0.514796 8.4:-59800488 MS.gene92438:CDS
GCTCAGCAAAATTTGTTGGT+TGG 0.517823 8.4:-59799951 MS.gene92438:CDS
CTTTGCTATAGTGTTAAAGC+TGG 0.518454 8.4:-59800119 MS.gene92438:CDS
CTCTAATTTGAAATCTGTTG+TGG 0.519015 8.4:-59800192 MS.gene92438:CDS
TGGGGATAATTCACATTCAG+AGG 0.526471 8.4:-59800663 MS.gene92438:CDS
TAGATTGAGGCCTATTTGCA+AGG 0.527767 8.4:+59800951 None:intergenic
ACATTTGTTCCAGTTGCTGA+TGG 0.528758 8.4:-59800029 MS.gene92438:CDS
ACATTGGAAGCATTTAGTGT+TGG 0.529902 8.4:-59800308 MS.gene92438:CDS
TGACATAGCTTTGATGGGCA+AGG 0.531421 8.4:+59800743 None:intergenic
TGAAACTGTAAGTCCATTGG+TGG 0.538274 8.4:+59800607 None:intergenic
AGATGACGCCAATTTGAGAT+GGG 0.541027 8.4:+59799985 None:intergenic
AAGATGACGCCAATTTGAGA+TGG 0.541117 8.4:+59799984 None:intergenic
GGACGGATTAAACATAGGAC+TGG 0.549325 8.4:+59800790 None:intergenic
ACAAATGGAAAATAATAGAA+GGG 0.553964 8.4:+59801141 None:intergenic
CAGATTGACATAGCTTTGAT+GGG 0.556288 8.4:+59800738 None:intergenic
TGAATATTCTACTTATTATG+GGG 0.558847 8.4:-59800681 MS.gene92438:CDS
CAAAGGTCATATGTTTGACT+GGG 0.559861 8.4:+59800137 None:intergenic
AAATGAAACTGTAAGTCCAT+TGG 0.564165 8.4:+59800604 None:intergenic
TTTGCTATAGTGTTAAAGCT+GGG 0.564562 8.4:-59800118 MS.gene92438:CDS
AATTGGAGGACCTTCAAATG+AGG 0.564581 8.4:-59800270 MS.gene92438:CDS
TTATATGGTATTGCTGATAC+CGG 0.565680 8.4:-59800866 MS.gene92438:CDS
GATTTGGACGGATTAAACAT+AGG 0.566692 8.4:+59800785 None:intergenic
TTGTAACTTGACGATTTGGA+CGG 0.568255 8.4:+59800773 None:intergenic
TTGCTATAGTGTTAAAGCTG+GGG 0.572032 8.4:-59800117 MS.gene92438:CDS
TGCTATAGTGTTAAAGCTGG+GGG 0.572883 8.4:-59800116 MS.gene92438:CDS
AAGCCAAACAATATCACTAC+CGG 0.582306 8.4:+59800847 None:intergenic
AATAAAAGAGTAGAAATTGG+AGG 0.589379 8.4:-59800284 MS.gene92438:CDS
TCTTATGAAGGCATAAACTC+TGG 0.590331 8.4:-59800542 MS.gene92438:CDS
TGGTTGTAACATTGTTGACA+AGG 0.591014 8.4:+59800815 None:intergenic
AAGACGCGGCTCCACCTCCA+AGG 0.597864 8.4:+59800510 None:intergenic
ATTGGAGGACCTTCAAATGA+GGG 0.598213 8.4:-59800269 MS.gene92438:CDS
GGGGATAATTCACATTCAGA+GGG 0.603075 8.4:-59800662 MS.gene92438:CDS
ATACCATATAACTTAAATGG+TGG 0.607968 8.4:+59800878 None:intergenic
AGATTGAGGCCTATTTGCAA+GGG 0.608899 8.4:+59800952 None:intergenic
ATCAAAGCTATGTCAATCTG+TGG 0.613992 8.4:-59800735 MS.gene92438:CDS
ACGGTTGATGGAACGACGTG+CGG 0.614424 8.4:+59800997 None:intergenic
CAGCTTTAACACTATAGCAA+AGG 0.616318 8.4:+59800120 None:intergenic
TCAGGGATTACAGTAGATTG+AGG 0.616599 8.4:+59800938 None:intergenic
CTTTGTAGTAATGATTGGCA+CGG 0.624849 8.4:+59800978 None:intergenic
GGCTGTGGTATCTGGTGACG+GGG 0.627189 8.4:-59800381 MS.gene92438:CDS
TATCTCATGACCTATTCAGT+TGG 0.628251 8.4:-59800905 MS.gene92438:CDS
CTTACATTGGAATCCACCAA+TGG 0.633185 8.4:-59800620 MS.gene92438:CDS
TGAGATACTCGCCGTCATCA+GGG 0.634463 8.4:+59800921 None:intergenic
ATCCGCACCTTTAAAATGTG+CGG 0.635428 8.4:+59800073 None:intergenic
TCTACTGTAATCCCTGATGA+CGG 0.647222 8.4:-59800932 MS.gene92438:CDS
GATGACGCCAATTTGAGATG+GGG 0.668669 8.4:+59799986 None:intergenic
TGAGGATGTGATAAAAGACG+CGG 0.717968 8.4:+59800496 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGAAATAATGAAACAAA+TGG + chr8.4:59799933-59799952 None:intergenic 15.0%
!! AACAAATGGAAAATAATAGA+AGG + chr8.4:59799919-59799938 None:intergenic 20.0%
!! AACAATATTATGTCTTATGA+AGG - chr8.4:59800502-59800521 MS.gene92438:CDS 20.0%
!! ACAAATGGAAAATAATAGAA+GGG + chr8.4:59799918-59799937 None:intergenic 20.0%
!! GTGAATATTCTACTTATTAT+GGG - chr8.4:59800374-59800393 MS.gene92438:CDS 20.0%
!! TCAAATAGTAAAAGGTTATT+GGG + chr8.4:59800729-59800748 None:intergenic 20.0%
!! TGAATATTCTACTTATTATG+GGG - chr8.4:59800375-59800394 MS.gene92438:CDS 20.0%
!! TGTGAATATTCTACTTATTA+TGG - chr8.4:59800373-59800392 MS.gene92438:CDS 20.0%
!!! TCATTTTCTAATATTGTGAT+AGG - chr8.4:59800469-59800488 MS.gene92438:CDS 20.0%
! AATAAAAGAGTAGAAATTGG+AGG - chr8.4:59800772-59800791 MS.gene92438:CDS 25.0%
! ATACCATATAACTTAAATGG+TGG + chr8.4:59800181-59800200 None:intergenic 25.0%
! GCAATACCATATAACTTAAA+TGG + chr8.4:59800184-59800203 None:intergenic 25.0%
! GGAAATAAAAGAGTAGAAAT+TGG - chr8.4:59800769-59800788 MS.gene92438:CDS 25.0%
! GTCAAATAGTAAAAGGTTAT+TGG + chr8.4:59800730-59800749 None:intergenic 25.0%
! TTCCAATGTCAAATAGTAAA+AGG + chr8.4:59800737-59800756 None:intergenic 25.0%
!! AACCTTTTACTATTTGACAT+TGG - chr8.4:59800732-59800751 MS.gene92438:CDS 25.0%
!! ATAATTTTTGTCATTACACG+AGG + chr8.4:59800353-59800372 None:intergenic 25.0%
!! ATTTTTGTAACTTGACGATT+TGG + chr8.4:59800290-59800309 None:intergenic 25.0%
!! TTTTCTCATACTCAAAAGAA+TGG - chr8.4:59799950-59799969 MS.gene92438:CDS 25.0%
!!! TATTGGTATTTGTTTTGAGT+AGG + chr8.4:59800028-59800047 None:intergenic 25.0%
AAATGAAACTGTAAGTCCAT+TGG + chr8.4:59800455-59800474 None:intergenic 30.0%
GGAAACTTTGTAGTAATGAT+TGG + chr8.4:59800086-59800105 None:intergenic 30.0%
GTGTTTGTCACAAAAAACAA+TGG + chr8.4:59800616-59800635 None:intergenic 30.0%
TCTAATATTGTGATAGGATG+TGG - chr8.4:59800475-59800494 MS.gene92438:CDS 30.0%
TTATATGGTATTGCTGATAC+CGG - chr8.4:59800190-59800209 MS.gene92438:CDS 30.0%
! ACTCCACCATTTAAGTTATA+TGG - chr8.4:59800175-59800194 MS.gene92438:CDS 30.0%
! CTCTAATTTGAAATCTGTTG+TGG - chr8.4:59800864-59800883 MS.gene92438:CDS 30.0%
! TCTTTTATCACATCCTCAAT+TGG - chr8.4:59800565-59800584 MS.gene92438:CDS 30.0%
! TTTGCTATAGTGTTAAAGCT+GGG - chr8.4:59800938-59800957 MS.gene92438:CDS 30.0%
!! GGTAACATCATTATTGATTC+AGG - chr8.4:59800808-59800827 MS.gene92438:CDS 30.0%
!! TTTTGAGTAGGTTTATAGAG+TGG + chr8.4:59800016-59800035 None:intergenic 30.0%
!!! ATTGGGTCTTTTTTCACTAT+AGG + chr8.4:59800712-59800731 None:intergenic 30.0%
AAGCCAAACAATATCACTAC+CGG + chr8.4:59800212-59800231 None:intergenic 35.0%
ACAAAGTTTCCCTTGCAAAT+AGG - chr8.4:59800095-59800114 MS.gene92438:CDS 35.0%
ACTGGAACAAATGTGCTAAT+AGG + chr8.4:59801021-59801040 None:intergenic 35.0%
AGAGAATTTACCACCAATTG+AGG + chr8.4:59800581-59800600 None:intergenic 35.0%
ATACCGGTAGTGATATTGTT+TGG - chr8.4:59800206-59800225 MS.gene92438:CDS 35.0%
ATCAAAGCTATGTCAATCTG+TGG - chr8.4:59800321-59800340 MS.gene92438:CDS 35.0%
ATGATGTTACCCTCATTTGA+AGG + chr8.4:59800799-59800818 None:intergenic 35.0%
CAAAGGTCATATGTTTGACT+GGG + chr8.4:59800922-59800941 None:intergenic 35.0%
CTGGAACAAATGTGCTAATA+GGG + chr8.4:59801020-59801039 None:intergenic 35.0%
CTTTGTAGTAATGATTGGCA+CGG + chr8.4:59800081-59800100 None:intergenic 35.0%
GAAATCTGTTGTGGTAGATT+TGG - chr8.4:59800873-59800892 MS.gene92438:CDS 35.0%
GGTACAATCACTAGGTTTAA+AGG + chr8.4:59801157-59801176 None:intergenic 35.0%
TATCTCATGACCTATTCAGT+TGG - chr8.4:59800151-59800170 MS.gene92438:CDS 35.0%
TGGTTGTAACATTGTTGACA+AGG + chr8.4:59800244-59800263 None:intergenic 35.0%
TTAAAATGTGCGGTGATTAG+AGG + chr8.4:59800976-59800995 None:intergenic 35.0%
TTGCTATAGTGTTAAAGCTG+GGG - chr8.4:59800939-59800958 MS.gene92438:CDS 35.0%
TTTATCACATCCTCAATTGG+TGG - chr8.4:59800568-59800587 MS.gene92438:CDS 35.0%
! CAGCTTTAACACTATAGCAA+AGG + chr8.4:59800939-59800958 None:intergenic 35.0%
! CTAATCACCGCACATTTTAA+AGG - chr8.4:59800976-59800995 MS.gene92438:CDS 35.0%
! CTTTGCTATAGTGTTAAAGC+TGG - chr8.4:59800937-59800956 MS.gene92438:CDS 35.0%
! TCTTATGAAGGCATAAACTC+TGG - chr8.4:59800514-59800533 MS.gene92438:CDS 35.0%
! TTGTAACTTGACGATTTGGA+CGG + chr8.4:59800286-59800305 None:intergenic 35.0%
! TTTGGCTCAGCAAAATTTGT+TGG - chr8.4:59801101-59801120 MS.gene92438:CDS 35.0%
!! ACAGATTGACATAGCTTTGA+TGG + chr8.4:59800322-59800341 None:intergenic 35.0%
!! ACATTGGAAGCATTTAGTGT+TGG - chr8.4:59800748-59800767 MS.gene92438:CDS 35.0%
!! CACATTTTGGTACAATCACT+AGG + chr8.4:59801165-59801184 None:intergenic 35.0%
!! CAGATTGACATAGCTTTGAT+GGG + chr8.4:59800321-59800340 None:intergenic 35.0%
!! GATTAAACATAGGACTGGTT+TGG + chr8.4:59800264-59800283 None:intergenic 35.0%
!! GATTTGGACGGATTAAACAT+AGG + chr8.4:59800274-59800293 None:intergenic 35.0%
!! TGGTGTTGATACTCTTACAT+TGG - chr8.4:59800423-59800442 MS.gene92438:CDS 35.0%
AAGATGACGCCAATTTGAGA+TGG + chr8.4:59801075-59801094 None:intergenic 40.0%
AATTGGAGGACCTTCAAATG+AGG - chr8.4:59800786-59800805 MS.gene92438:CDS 40.0%
ACATTTGTTCCAGTTGCTGA+TGG - chr8.4:59801027-59801046 MS.gene92438:CDS 40.0%
AGATTGAGGCCTATTTGCAA+GGG + chr8.4:59800107-59800126 None:intergenic 40.0%
ATTGGAGGACCTTCAAATGA+GGG - chr8.4:59800787-59800806 MS.gene92438:CDS 40.0%
CAATTAACACCATCAGCAAC+TGG + chr8.4:59801039-59801058 None:intergenic 40.0%
CTTACATTGGAATCCACCAA+TGG - chr8.4:59800436-59800455 MS.gene92438:CDS 40.0%
GCAAAGGTCATATGTTTGAC+TGG + chr8.4:59800923-59800942 None:intergenic 40.0%
GGGGATAATTCACATTCAGA+GGG - chr8.4:59800394-59800413 MS.gene92438:CDS 40.0%
TAGATTGAGGCCTATTTGCA+AGG + chr8.4:59800108-59800127 None:intergenic 40.0%
TCAGGGATTACAGTAGATTG+AGG + chr8.4:59800121-59800140 None:intergenic 40.0%
TCTACTGTAATCCCTGATGA+CGG - chr8.4:59800124-59800143 MS.gene92438:CDS 40.0%
TCTCAAATTGGCGTCATCTT+TGG - chr8.4:59801075-59801094 MS.gene92438:CDS 40.0%
TGAAACTGTAAGTCCATTGG+TGG + chr8.4:59800452-59800471 None:intergenic 40.0%
TGAGGATGTGATAAAAGACG+CGG + chr8.4:59800563-59800582 None:intergenic 40.0%
TGCTATAGTGTTAAAGCTGG+GGG - chr8.4:59800940-59800959 MS.gene92438:CDS 40.0%
TGGGGATAATTCACATTCAG+AGG - chr8.4:59800393-59800412 MS.gene92438:CDS 40.0%
! AGATGACGCCAATTTGAGAT+GGG + chr8.4:59801074-59801093 None:intergenic 40.0%
! TGCTGATGGTGTTAATTGCT+TGG - chr8.4:59801041-59801060 MS.gene92438:CDS 40.0%
! TGGCGTCATCTTTGGAAATT+TGG - chr8.4:59801083-59801102 MS.gene92438:CDS 40.0%
!! ATCCGCACCTTTAAAATGTG+CGG + chr8.4:59800986-59801005 None:intergenic 40.0%
!! CAAGCTCAATTTTGGAGATG+CGG - chr8.4:59800654-59800673 MS.gene92438:CDS 40.0%
!! GCTCAGCAAAATTTGTTGGT+TGG - chr8.4:59801105-59801124 MS.gene92438:CDS 40.0%
!! GTGCGGCATTGACAAAATAT+TGG + chr8.4:59800045-59800064 None:intergenic 40.0%
!!! GTGGTGATTTTAAAGTGTCG+CGG + chr8.4:59799997-59800016 None:intergenic 40.0%
GGCATAAACTCTGGCATAGT+TGG - chr8.4:59800523-59800542 MS.gene92438:CDS 45.0%
GTAATGATTGGCACGGTTGA+TGG + chr8.4:59800074-59800093 None:intergenic 45.0%
TCACATTCAGAGGGAGATCT+TGG - chr8.4:59800403-59800422 MS.gene92438:CDS 45.0%
! ACGACGAGCAAGCTCAATTT+TGG - chr8.4:59800646-59800665 MS.gene92438:CDS 45.0%
! GATGACGCCAATTTGAGATG+GGG + chr8.4:59801073-59801092 None:intergenic 45.0%
! GCTTTTGCCCCATCTCAAAT+TGG - chr8.4:59801063-59801082 MS.gene92438:CDS 45.0%
! TGGTGGAGTACCAACTGAAT+AGG + chr8.4:59800164-59800183 None:intergenic 45.0%
!! CACCGCACATTTTAAAGGTG+CGG - chr8.4:59800981-59801000 MS.gene92438:CDS 45.0%
!! GGACGGATTAAACATAGGAC+TGG + chr8.4:59800269-59800288 None:intergenic 45.0%
!! TGACATAGCTTTGATGGGCA+AGG + chr8.4:59800316-59800335 None:intergenic 45.0%
!!! GTGATTTTAAAGTGTCGCGG+TGG + chr8.4:59799994-59800013 None:intergenic 45.0%
ATGAGATACTCGCCGTCATC+AGG + chr8.4:59800139-59800158 None:intergenic 50.0%
TGAGATACTCGCCGTCATCA+GGG + chr8.4:59800138-59800157 None:intergenic 50.0%
! AACTCTGGCATAGTTGGCCT+TGG - chr8.4:59800529-59800548 MS.gene92438:CDS 50.0%
!! CAATTTTGGAGATGCGGCTG+TGG - chr8.4:59800660-59800679 MS.gene92438:CDS 50.0%
!! ACGGTTGATGGAACGACGTG+CGG + chr8.4:59800062-59800081 None:intergenic 55.0%
!! TCTGGCATAGTTGGCCTTGG+AGG - chr8.4:59800532-59800551 MS.gene92438:CDS 55.0%
CGGCTGTGGTATCTGGTGAC+GGG - chr8.4:59800674-59800693 MS.gene92438:CDS 60.0%
GCGGCTGTGGTATCTGGTGA+CGG - chr8.4:59800673-59800692 MS.gene92438:CDS 60.0%
GGAGATGCGGCTGTGGTATC+TGG - chr8.4:59800667-59800686 MS.gene92438:CDS 60.0%
! GGCTGTGGTATCTGGTGACG+GGG - chr8.4:59800675-59800694 MS.gene92438:CDS 60.0%
!! GGCATAGTTGGCCTTGGAGG+TGG - chr8.4:59800535-59800554 MS.gene92438:CDS 60.0%
AAGACGCGGCTCCACCTCCA+AGG + chr8.4:59800549-59800568 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 59799893 59801185 59799893 ID=MS.gene92438
chr8.4 mRNA 59799893 59801185 59799893 ID=MS.gene92438.t1;Parent=MS.gene92438
chr8.4 exon 59799893 59801185 59799893 ID=MS.gene92438.t1.exon1;Parent=MS.gene92438.t1
chr8.4 CDS 59799893 59801185 59799893 ID=cds.MS.gene92438.t1;Parent=MS.gene92438.t1
Gene Sequence

>MS.gene92438

ATGAGCAAACGTTCATTTCTTACCCTTCTATTATTTTCCATTTGTTTCATTATTTCTTTTTCTCATACTCAAAAGAATGGTTTTAGTGTTGAACTCATCCACCGCGACACTTTAAAATCACCACTCTATAAACCTACTCAAAACAAATACCAATATTTTGTCAATGCCGCACGTCGTTCCATCAACCGTGCCAATCATTACTACAAAGTTTCCCTTGCAAATAGGCCTCAATCTACTGTAATCCCTGATGACGGCGAGTATCTCATGACCTATTCAGTTGGTACTCCACCATTTAAGTTATATGGTATTGCTGATACCGGTAGTGATATTGTTTGGCTTCAATGTGAGCCTTGTCAACAATGTTACAACCAAACCAGTCCTATGTTTAATCCGTCCAAATCGTCAAGTTACAAAAATATTCCTTGCCCATCAAAGCTATGTCAATCTGTGGAAGATACCTCGTGTAATGACAAAAATTATTGTGAATATTCTACTTATTATGGGGATAATTCACATTCAGAGGGAGATCTTGGTGTTGATACTCTTACATTGGAATCCACCAATGGACTTACAGTTTCATTTTCTAATATTGTGATAGGATGTGGAACTAACAATATTATGTCTTATGAAGGCATAAACTCTGGCATAGTTGGCCTTGGAGGTGGAGCCGCGTCTTTTATCACATCCTCAATTGGTGGTAAATTCTCTTATTGTTTAACTCCATTGTTTTTTGTGACAAACACTGAGTCAAACACGACGAGCAAGCTCAATTTTGGAGATGCGGCTGTGGTATCTGGTGACGGGGTTGTGACAACTCCTATAGTGAAAAAAGACCCAATAACCTTTTACTATTTGACATTGGAAGCATTTAGTGTTGGAAATAAAAGAGTAGAAATTGGAGGACCTTCAAATGAGGGTAACATCATTATTGATTCAGGTACAACATTGACACTTTTAACAAATGATGATTACTCTAATTTGAAATCTGTTGTGGTAGATTTGGTGAAATTAGAACGTGTTGACGATCCCAGTCAAACATATGACCTTTGCTATAGTGTTAAAGCTGGGGGATATGATTTTCCTCTAATCACCGCACATTTTAAAGGTGCGGATGTGAATTTGCACCCTATTAGCACATTTGTTCCAGTTGCTGATGGTGTTAATTGCTTGGCTTTTGCCCCATCTCAAATTGGCGTCATCTTTGGAAATTTGGCTCAGCAAAATTTGTTGGTTGGTTATGATCTTCAGCAAAAAACTGTGTCCTTTAAACCTAGTGATTGTACCAAAATGTGA
Protein sequence

>MS.gene92438.t1

MSKRSFLTLLLFSICFIISFSHTQKNGFSVELIHRDTLKSPLYKPTQNKYQYFVNAARRSINRANHYYKVSLANRPQSTVIPDDGEYLMTYSVGTPPFKLYGIADTGSDIVWLQCEPCQQCYNQTSPMFNPSKSSSYKNIPCPSKLCQSVEDTSCNDKNYCEYSTYYGDNSHSEGDLGVDTLTLESTNGLTVSFSNIVIGCGTNNIMSYEGINSGIVGLGGGAASFITSSIGGKFSYCLTPLFFVTNTESNTTSKLNFGDAAVVSGDGVVTTPIVKKDPITFYYLTLEAFSVGNKRVEIGGPSNEGNIIIDSGTTLTLLTNDDYSNLKSVVVDLVKLERVDDPSQTYDLCYSVKAGGYDFPLITAHFKGADVNLHPISTFVPVADGVNCLAFAPSQIGVIFGNLAQQNLLVGYDLQQKTVSFKPSDCTKM