AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92914

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92914.t1 MTR_5g044770 95.918 343 14 0 1 343 89 431 0.0 676
MS.gene92914.t1 MTR_8g096990 74.419 344 87 1 1 343 89 432 0.0 526
MS.gene92914.t1 MTR_5g080960 73.199 347 88 4 1 343 89 434 0.0 511
MS.gene92914.t1 MTR_5g080890 72.622 347 90 4 1 343 89 434 0.0 509
MS.gene92914.t1 MTR_8g038280 70.115 348 99 2 1 343 89 436 6.70e-176 495
MS.gene92914.t1 MTR_8g038250 68.571 350 81 3 1 343 89 416 5.73e-167 472
MS.gene92914.t1 MTR_4g109760 71.903 331 89 3 15 343 71 399 1.61e-159 452
MS.gene92914.t1 MTR_6g090290 64.655 348 117 3 1 342 89 436 7.72e-154 439
MS.gene92914.t1 MTR_8g038310 63.848 343 104 4 1 342 90 413 5.73e-151 431
MS.gene92914.t1 MTR_3g028120 55.172 348 151 3 1 343 92 439 3.33e-131 382
MS.gene92914.t1 MTR_2g067830 54.755 347 152 4 1 342 60 406 1.80e-124 363
MS.gene92914.t1 MTR_5g038360 51.437 348 161 4 1 342 91 436 4.39e-122 358
MS.gene92914.t1 MTR_0024s0040 53.736 348 152 5 1 342 66 410 2.08e-121 356
MS.gene92914.t1 MTR_2g105300 54.268 328 145 4 17 339 54 381 2.26e-117 344
MS.gene92914.t1 MTR_6g088760 54.375 320 138 5 1 313 91 409 4.90e-111 333
MS.gene92914.t1 MTR_5g044570 49.568 347 143 6 1 342 92 411 7.22e-111 329
MS.gene92914.t1 MTR_2g059490 46.591 352 178 7 1 343 61 411 7.61e-99 299
MS.gene92914.t1 MTR_5g008070 43.314 344 190 5 1 341 84 425 3.52e-91 279
MS.gene92914.t1 MTR_0036s0050 44.286 350 180 8 1 342 93 435 5.94e-91 279
MS.gene92914.t1 MTR_5g008150 43.429 350 187 8 1 342 66 412 2.38e-90 276
MS.gene92914.t1 MTR_2g059460 43.182 352 183 9 1 342 62 406 2.18e-89 274
MS.gene92914.t1 MTR_0036s0070 41.449 345 188 7 1 342 93 426 7.91e-89 273
MS.gene92914.t1 MTR_5g008110 42.286 350 190 7 1 342 70 415 1.45e-88 272
MS.gene92914.t1 MTR_0036s0110 40.395 354 191 8 1 342 97 442 1.10e-81 255
MS.gene92914.t1 MTR_3g097150 38.810 353 198 9 1 343 105 449 6.65e-74 235
MS.gene92914.t1 MTR_1g060750 37.576 330 187 10 23 342 56 376 3.16e-65 211
MS.gene92914.t1 MTR_4g045857 46.053 228 117 4 1 222 61 288 2.03e-62 201
MS.gene92914.t1 MTR_5g008090 39.057 297 170 7 54 342 21 314 1.06e-61 200
MS.gene92914.t1 MTR_1g115210 37.971 345 194 11 5 340 153 486 8.29e-60 199
MS.gene92914.t1 MTR_1g039270 35.920 348 198 11 5 340 135 469 6.55e-56 189
MS.gene92914.t1 MTR_7g117430 34.943 352 198 12 5 340 148 484 6.75e-56 189
MS.gene92914.t1 MTR_6g085010 34.211 342 206 10 5 340 163 491 1.96e-50 175
MS.gene92914.t1 MTR_6g088765 40.625 256 111 5 72 326 3 218 9.14e-50 166
MS.gene92914.t1 MTR_2g028190 34.571 350 208 10 5 340 109 451 5.91e-48 167
MS.gene92914.t1 MTR_1g111350 34.104 346 202 12 5 340 138 467 6.73e-48 168
MS.gene92914.t1 MTR_3g046300 32.869 359 220 9 5 343 104 461 1.64e-46 164
MS.gene92914.t1 MTR_4g007740 33.241 361 200 13 4 341 136 478 9.35e-46 162
MS.gene92914.t1 MTR_3g053710 33.523 352 209 12 5 340 124 466 7.34e-42 152
MS.gene92914.t1 MTR_5g091130 30.769 364 230 10 1 343 194 556 2.20e-41 152
MS.gene92914.t1 MTR_8g103880 30.178 338 215 12 5 333 123 448 1.12e-35 135
MS.gene92914.t1 MTR_2g070430 29.730 370 224 12 4 342 93 457 1.56e-33 129
MS.gene92914.t1 MTR_2g055840 28.142 366 221 13 5 340 20 373 2.30e-33 127
MS.gene92914.t1 MTR_3g068000 29.581 382 211 14 1 340 76 441 1.00e-32 127
MS.gene92914.t1 MTR_4g008110 52.586 116 32 1 70 185 45 137 9.27e-29 108
MS.gene92914.t1 MTR_3g053980 31.339 351 212 12 5 340 149 485 1.85e-28 115
MS.gene92914.t1 MTR_8g075090 29.477 363 213 16 4 342 118 461 4.41e-28 114
MS.gene92914.t1 MTR_7g080230 28.142 366 222 14 5 341 78 431 9.36e-28 113
MS.gene92914.t1 MTR_3g065160 25.613 367 227 11 5 343 81 429 1.57e-27 112
MS.gene92914.t1 MTR_8g075090 29.121 364 215 16 4 343 118 462 1.64e-27 112
MS.gene92914.t1 MTR_7g062360 27.937 383 220 16 1 343 62 428 4.22e-27 111
MS.gene92914.t1 MTR_4g095270 27.348 362 218 18 5 343 87 426 1.31e-26 110
MS.gene92914.t1 MTR_8g094590 27.200 375 218 17 1 343 76 427 1.63e-26 109
MS.gene92914.t1 MTR_4g095270 27.348 362 218 18 5 343 87 426 1.65e-26 110
MS.gene92914.t1 MTR_8g075090 30.267 337 192 16 4 316 118 435 1.48e-25 107
MS.gene92914.t1 MTR_5g016260 25.275 364 223 17 5 343 95 434 2.11e-25 107
MS.gene92914.t1 MTR_5g016260 25.275 364 223 17 5 343 95 434 2.52e-25 107
MS.gene92914.t1 MTR_5g022880 27.978 361 219 13 4 340 118 461 4.04e-25 106
MS.gene92914.t1 MTR_1g061940 29.670 364 221 15 5 341 90 445 1.70e-24 104
MS.gene92914.t1 MTR_7g105850 29.365 378 218 16 6 342 98 467 2.26e-24 103
MS.gene92914.t1 MTR_8g094590 28.977 352 202 17 1 328 76 403 3.06e-24 102
MS.gene92914.t1 MTR_1g078240 27.248 367 225 15 5 341 76 430 4.19e-24 102
MS.gene92914.t1 MTR_6g084710 27.297 370 218 17 5 340 198 550 4.21e-24 103
MS.gene92914.t1 MTR_6g084710 30.035 283 170 13 4 275 197 462 8.90e-24 102
MS.gene92914.t1 MTR_1g078240 27.446 368 224 16 5 341 76 431 1.12e-23 101
MS.gene92914.t1 MTR_2g084550 30.959 365 214 15 5 341 84 438 1.54e-23 101
MS.gene92914.t1 MTR_8g094590 30.588 255 147 10 1 245 76 310 8.99e-23 97.1
MS.gene92914.t1 MTR_7g021510 26.902 368 226 16 5 341 79 434 1.00e-22 99.0
MS.gene92914.t1 MTR_3g087780 28.493 365 212 18 5 340 106 450 1.44e-22 99.0
MS.gene92914.t1 MTR_7g102750 29.570 372 209 20 5 341 88 441 4.63e-22 97.1
MS.gene92914.t1 MTR_1g029810 26.005 373 226 17 1 343 60 412 2.73e-21 94.4
MS.gene92914.t1 MTR_4g056420 28.571 364 223 16 5 341 84 437 3.00e-21 94.7
MS.gene92914.t1 MTR_1g029810 26.005 373 226 17 1 343 60 412 3.22e-21 94.4
MS.gene92914.t1 MTR_8g075010 25.683 366 231 13 2 343 104 452 7.94e-21 93.6
MS.gene92914.t1 MTR_5g061690 29.226 349 199 20 5 328 108 433 1.63e-20 92.8
MS.gene92914.t1 MTR_4g100990 25.823 395 225 17 9 343 87 473 3.08e-20 91.7
MS.gene92914.t1 MTR_4g023650 25.926 378 226 17 1 343 64 422 4.14e-20 90.9
MS.gene92914.t1 MTR_1g078240 27.575 301 184 12 5 283 76 364 5.21e-20 90.5
MS.gene92914.t1 MTR_0945s0010 38.095 126 74 3 221 343 1 125 5.57e-20 85.9
MS.gene92914.t1 MTR_5g077850 25.464 377 227 11 1 343 84 440 1.29e-19 89.7
MS.gene92914.t1 MTR_3g014290 27.586 348 224 12 5 341 87 417 2.77e-19 88.6
MS.gene92914.t1 MTR_7g080230 26.361 349 216 14 22 341 25 361 4.74e-19 87.8
MS.gene92914.t1 MTR_1g037630 27.586 348 224 12 5 341 99 429 5.81e-19 87.8
MS.gene92914.t1 MTR_7g102750 29.912 341 187 19 5 311 88 410 5.94e-19 87.8
MS.gene92914.t1 MTR_2g063110 24.615 390 236 15 4 340 124 508 1.03e-18 87.4
MS.gene92914.t1 MTR_3g087780 29.154 319 188 16 41 340 9 308 1.89e-18 85.9
MS.gene92914.t1 MTR_3g087780 29.154 319 188 16 41 340 45 344 1.99e-18 86.3
MS.gene92914.t1 MTR_8g039540 27.089 347 226 12 6 341 104 434 6.12e-17 81.6
MS.gene92914.t1 MTR_4g023630 23.936 376 236 16 1 343 64 422 7.34e-17 81.6
MS.gene92914.t1 MTR_2g103340 82.051 39 7 0 10 48 67 105 1.14e-16 77.4
MS.gene92914.t1 MTR_2g103340 45.455 77 37 2 261 337 88 159 1.13e-11 63.5
MS.gene92914.t1 MTR_5g061690 28.912 294 172 18 50 328 23 294 2.65e-15 76.6
MS.gene92914.t1 MTR_5g077920 25.606 371 234 11 1 342 71 428 3.42e-14 73.6
MS.gene92914.t1 MTR_2g037710 28.293 205 124 7 142 340 18 205 2.10e-12 65.9
MS.gene92914.t1 MTR_3g100500 26.923 338 215 13 5 332 104 419 2.90e-11 64.7
MS.gene92914.t1 MTR_3g006820 22.656 384 227 16 1 343 61 415 3.99e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92914.t1 AT1G64830 51.304 345 165 3 1 343 88 431 2.54e-119 351
MS.gene92914.t1 AT5G33340 51.009 347 165 5 1 343 92 437 7.10e-111 330
MS.gene92914.t1 AT2G35615 46.927 358 173 6 1 341 87 444 4.11e-100 303
MS.gene92914.t1 AT1G31450 43.820 356 185 5 1 341 87 442 1.72e-90 278
MS.gene92914.t1 AT2G28010 44.286 350 166 10 1 343 67 394 4.18e-84 260
MS.gene92914.t1 AT2G28040 44.699 349 165 10 1 343 58 384 1.71e-81 253
MS.gene92914.t1 AT2G28040 44.699 349 165 10 1 343 67 393 2.37e-81 253
MS.gene92914.t1 AT2G28030 43.429 350 169 10 1 343 63 390 2.65e-79 248
MS.gene92914.t1 AT2G28225 42.373 354 171 11 1 343 69 400 5.78e-79 247
MS.gene92914.t1 AT2G28220 42.938 354 169 11 1 343 69 400 1.43e-78 246
MS.gene92914.t1 AT2G03200 38.674 362 194 11 1 343 109 461 5.21e-67 218
MS.gene92914.t1 AT1G01300 36.023 347 203 11 5 341 148 485 2.07e-55 188
MS.gene92914.t1 AT3G18490 34.971 346 202 12 5 340 168 500 2.51e-54 186
MS.gene92914.t1 AT3G20015 34.971 346 203 13 5 340 137 470 1.09e-52 181
MS.gene92914.t1 AT4G30030 34.758 351 201 13 4 340 83 419 3.92e-50 173
MS.gene92914.t1 AT3G61820 35.014 357 205 13 1 340 137 483 7.45e-50 173
MS.gene92914.t1 AT1G25510 33.908 348 200 12 5 340 154 483 4.16e-48 169
MS.gene92914.t1 AT5G10770 34.844 353 206 12 1 341 134 474 4.31e-46 163
MS.gene92914.t1 AT5G10760 36.628 344 201 11 1 340 134 464 4.13e-45 160
MS.gene92914.t1 AT3G12700 32.670 352 220 10 5 341 112 461 7.84e-44 157
MS.gene92914.t1 AT2G23945 29.396 364 226 10 1 343 98 451 1.44e-43 156
MS.gene92914.t1 AT3G25700 29.508 366 224 13 5 341 90 450 2.08e-42 153
MS.gene92914.t1 AT4G30040 33.429 350 203 12 4 335 90 427 2.98e-41 149
MS.gene92914.t1 AT2G42980 31.707 369 223 14 1 343 162 527 2.72e-40 149
MS.gene92914.t1 AT1G79720 31.339 351 207 12 11 340 145 482 2.70e-39 145
MS.gene92914.t1 AT3G59080 30.518 367 228 12 1 343 172 535 2.79e-39 146
MS.gene92914.t1 AT5G36260 29.348 368 212 15 5 341 84 434 9.05e-35 133
MS.gene92914.t1 AT3G02740 28.962 366 215 16 5 341 16 365 1.51e-32 125
MS.gene92914.t1 AT3G02740 29.428 367 212 17 5 341 91 440 2.84e-32 126
MS.gene92914.t1 AT2G39710 28.533 375 212 14 1 335 67 425 5.58e-31 122
MS.gene92914.t1 AT3G59080 28.453 362 206 11 1 343 172 499 1.03e-29 119
MS.gene92914.t1 AT1G65240 28.610 367 213 17 5 341 80 427 7.11e-29 116
MS.gene92914.t1 AT5G02190 27.321 377 214 15 8 340 82 442 3.66e-27 111
MS.gene92914.t1 AT5G43100 27.072 362 218 16 5 343 82 420 2.50e-26 109
MS.gene92914.t1 AT5G43100 27.072 362 218 16 5 343 82 420 5.86e-26 109
MS.gene92914.t1 AT3G54400 27.273 352 221 13 5 341 94 425 3.01e-25 105
MS.gene92914.t1 AT5G37540 26.075 372 228 13 1 340 82 438 1.05e-24 104
MS.gene92914.t1 AT2G17760 27.298 359 221 14 4 340 109 449 3.81e-24 103
MS.gene92914.t1 AT1G44130 26.087 368 223 15 5 343 55 402 9.41e-24 101
MS.gene92914.t1 AT1G66180 26.810 373 226 13 1 343 74 429 1.38e-23 101
MS.gene92914.t1 AT1G49050 24.100 361 228 15 16 342 49 397 2.35e-23 100
MS.gene92914.t1 AT2G36670 28.533 368 217 19 5 341 40 392 2.61e-23 100
MS.gene92914.t1 AT2G36670 28.533 368 217 19 5 341 40 392 2.61e-23 100
MS.gene92914.t1 AT2G36670 28.533 368 217 19 5 341 106 458 3.39e-23 100
MS.gene92914.t1 AT2G36670 28.533 368 217 19 5 341 111 463 3.39e-23 100
MS.gene92914.t1 AT1G05840 26.630 368 225 16 5 341 86 439 3.79e-23 100
MS.gene92914.t1 AT3G50050 26.519 362 222 14 5 343 99 439 6.29e-23 100
MS.gene92914.t1 AT4G33490 25.661 378 221 17 1 343 62 414 6.49e-23 99.4
MS.gene92914.t1 AT1G09750 27.378 347 233 11 5 340 110 448 7.39e-23 99.4
MS.gene92914.t1 AT5G45120 27.182 401 224 18 1 340 85 478 8.15e-23 99.4
MS.gene92914.t1 AT1G49050 24.100 361 228 15 16 342 222 570 1.16e-22 99.4
MS.gene92914.t1 AT5G22850 30.831 373 204 19 5 341 87 441 3.76e-22 97.4
MS.gene92914.t1 AT3G52500 29.306 389 215 19 1 341 92 468 8.00e-21 93.6
MS.gene92914.t1 AT3G51350 26.446 363 224 16 4 340 107 452 1.94e-20 92.4
MS.gene92914.t1 AT4G33490 27.425 299 174 14 1 281 59 332 2.15e-20 91.7
MS.gene92914.t1 AT3G51360 28.571 364 211 18 4 340 10 351 2.22e-20 91.7
MS.gene92914.t1 AT4G12920 26.667 345 221 14 6 340 65 387 2.57e-20 91.3
MS.gene92914.t1 AT1G49050 27.642 246 158 10 16 250 222 458 2.80e-20 92.0
MS.gene92914.t1 AT3G51360 28.571 364 211 18 4 340 94 435 3.56e-20 91.7
MS.gene92914.t1 AT1G08210 29.076 368 214 18 5 341 90 441 3.58e-20 91.7
MS.gene92914.t1 AT3G51360 28.571 364 211 18 4 340 104 445 3.61e-20 91.7
MS.gene92914.t1 AT1G08210 29.076 368 214 18 5 341 124 475 5.09e-20 91.3
MS.gene92914.t1 AT1G08210 29.076 368 214 18 5 341 138 489 5.11e-20 91.3
MS.gene92914.t1 AT5G10080 27.128 376 210 22 5 342 106 455 4.14e-18 85.5
MS.gene92914.t1 AT4G35880 25.138 362 234 14 2 343 110 454 6.71e-18 85.1
MS.gene92914.t1 AT3G51360 29.448 326 183 17 4 304 94 397 2.19e-17 83.2
MS.gene92914.t1 AT3G51360 29.448 326 183 17 4 304 104 407 2.24e-17 83.2
MS.gene92914.t1 AT3G51360 28.763 299 174 13 4 283 94 372 1.05e-16 80.9
MS.gene92914.t1 AT3G51360 28.763 299 174 13 4 283 104 382 1.06e-16 80.9
MS.gene92914.t1 AT1G08210 29.394 330 188 17 5 305 90 403 2.15e-16 80.5
MS.gene92914.t1 AT5G07030 28.889 315 195 13 5 308 121 417 4.58e-16 79.3
MS.gene92914.t1 AT3G51340 25.281 356 231 14 4 340 108 447 1.37e-15 78.2
MS.gene92914.t1 AT3G51360 28.840 319 187 17 40 340 13 309 3.90e-15 75.9
MS.gene92914.t1 AT3G51360 28.840 319 187 17 40 340 13 309 3.90e-15 75.9
MS.gene92914.t1 AT4G35880 25.672 335 212 14 2 316 110 427 5.80e-15 75.9
MS.gene92914.t1 AT3G51330 25.895 363 227 16 4 340 107 453 6.26e-15 75.9
MS.gene92914.t1 AT3G51330 25.895 363 227 16 4 340 107 453 6.27e-15 76.3
MS.gene92914.t1 AT3G51360 28.740 254 151 10 4 244 104 340 2.60e-14 73.6
MS.gene92914.t1 AT3G51340 22.951 366 239 12 4 340 108 459 3.59e-14 73.9
MS.gene92914.t1 AT3G51340 25.267 281 180 10 4 268 108 374 1.38e-13 71.6
MS.gene92914.t1 AT3G51340 25.490 306 196 12 4 292 108 398 1.94e-13 71.2
MS.gene92914.t1 AT3G25700 27.820 133 88 3 5 129 90 222 3.49e-13 70.1
MS.gene92914.t1 AT4G16563 25.062 403 219 17 16 343 100 494 5.25e-13 70.1
MS.gene92914.t1 AT3G42550 31.683 101 67 1 2 100 81 181 3.85e-11 64.3

Find 69 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGATGCCGCTATGGTTTC+TGG 0.152074 5.1:-33199947 MS.gene92914:CDS
AAGACGAGCAAACTCAATTT+TGG 0.203851 5.1:-33199968 MS.gene92914:CDS
TTAAAATGTGCAGTGATTAT+AGG 0.219892 5.1:+33199635 None:intergenic
ACATGAGCAAAGGTACTAAT+TGG 0.237962 5.1:+33199590 None:intergenic
ATACAGGTAGTGACATTATT+TGG 0.244218 5.1:-33200405 MS.gene92914:CDS
CAAAGGTTCAATAGTTGATT+AGG 0.274777 5.1:+33199689 None:intergenic
ACAACACCATATACATTAAA+AGG 0.278090 5.1:+33200430 None:intergenic
GGAAACAAAAGAATAGAATT+TGG 0.282532 5.1:-33199845 MS.gene92914:CDS
TCTCAAACTGGTCCCATCTT+TGG 0.292790 5.1:-33199533 MS.gene92914:CDS
TTACTGTTTGTTGCCGTTGA+TGG 0.300361 5.1:-33200002 MS.gene92914:CDS
TCATTTCCTAAAACCGTTAT+CGG 0.314542 5.1:-33200142 MS.gene92914:CDS
GGTATAGTTGGTCTTGGAAT+TGG 0.333564 5.1:-33200076 MS.gene92914:CDS
TGAGTATTTGATGACTTATT+CGG 0.350299 5.1:-33200464 None:intergenic
CTTGGCACAACTAAATTTGT+TGG 0.360015 5.1:-33199507 MS.gene92914:CDS
ATGTTTCAAGGGGCAACTTC+CGG 0.393396 5.1:-33200097 MS.gene92914:CDS
GGTAACATCATACTTGATTC+AGG 0.399481 5.1:-33199800 MS.gene92914:CDS
TATGTCTCTTACAACACAAT+TGG 0.409960 5.1:-33200050 MS.gene92914:CDS
ACACAATTGGAATCTTCAAT+TGG 0.412966 5.1:-33200037 MS.gene92914:CDS
AGAATCGGCGGTGGCGCAAT+TGG 0.424123 5.1:-33199735 MS.gene92914:CDS
TTGTGCCAAGTTTCCAAAGA+TGG 0.428148 5.1:+33199520 None:intergenic
TGGTCCCATCTTTGGAAACT+TGG 0.432754 5.1:-33199525 MS.gene92914:CDS
TGTTTATACTAATTTAGAAT+CGG 0.455605 5.1:-33199750 MS.gene92914:CDS
CCTAAAACCGTTATCGGATG+TGG 0.459444 5.1:-33200136 MS.gene92914:CDS
ACTTCCGGTATAGTTGGTCT+TGG 0.461194 5.1:-33200082 MS.gene92914:CDS
AATAATACAGGGATGTTTCA+AGG 0.462978 5.1:-33200109 MS.gene92914:CDS
TGCTTGTAGCATTGTTCACA+AGG 0.465026 5.1:+33200370 None:intergenic
GGATGTGGACACAATAATAC+AGG 0.478375 5.1:-33200121 MS.gene92914:CDS
ACCTTTGCTCATGTTGCTGA+TGG 0.482020 5.1:-33199581 MS.gene92914:CDS
AATTGTGTTGTAAGAGACAT+AGG 0.482732 5.1:+33200052 None:intergenic
ACATTAGAAGCATTTAGTGT+TGG 0.483347 5.1:-33199866 MS.gene92914:CDS
TGCTCATGTTGCTGATGGTG+TGG 0.491769 5.1:-33199576 MS.gene92914:CDS
TGAAACAGAATGACCGGTGG+TGG 0.491841 5.1:+33200162 None:intergenic
TAGTGTTGAGACTCTTACAT+TGG 0.503954 5.1:-33200188 MS.gene92914:CDS
GCACAACTAAATTTGTTGGT+TGG 0.505438 5.1:-33199503 MS.gene92914:CDS
ATTGTGTCCACATCCGATAA+CGG 0.507005 5.1:+33200129 None:intergenic
CTTACATTGGATTCCACCAC+CGG 0.521813 5.1:-33200175 MS.gene92914:CDS
CATGAGCAAAGGTACTAATT+GGG 0.521844 5.1:+33199591 None:intergenic
GTATATGGTGTTGTTGATAC+AGG 0.522915 5.1:-33200421 MS.gene92914:CDS
AGGAAATGAAACAGAATGAC+CGG 0.524965 5.1:+33200156 None:intergenic
GGGGCAACTTCCGGTATAGT+TGG 0.526062 5.1:-33200088 MS.gene92914:CDS
GCCGCTATGGTTTCTGGTGA+CGG 0.531191 5.1:-33199941 MS.gene92914:CDS
AACAAAAGAATAGAATTTGG+AGG 0.531892 5.1:-33199842 MS.gene92914:CDS
TGTGCCAAGTTTCCAAAGAT+GGG 0.540890 5.1:+33199521 None:intergenic
ACTCTAATTTCATTACACCT+TGG 0.549070 5.1:+33199433 None:intergenic
AATTCCAAGACCAACTATAC+CGG 0.550046 5.1:+33200078 None:intergenic
ATAATACAGGGATGTTTCAA+GGG 0.555636 5.1:-33200108 MS.gene92914:CDS
AGAGTTCTGTTCATTACAAG+AGG 0.561935 5.1:+33200261 None:intergenic
ACAAAAGAATAGAATTTGGA+GGG 0.566114 5.1:-33199841 MS.gene92914:CDS
CTGATGTGATACTATAACAA+AGG 0.576754 5.1:+33199672 None:intergenic
TGAAGGATTAAATATAGGAG+TGG 0.580266 5.1:+33200345 None:intergenic
GGGTTAGATGACAGCGAGGA+GGG 0.580976 5.1:-33199821 MS.gene92914:CDS
TACTAATTTAGAATCGGCGG+TGG 0.581102 5.1:-33199744 MS.gene92914:CDS
CAATTGGAATCTTCAATTGG+TGG 0.584460 5.1:-33200034 MS.gene92914:CDS
TGACATAAGTTAGAAGAGCA+AGG 0.597450 5.1:+33200298 None:intergenic
TACACCTTGGTACAATCACT+AGG 0.605033 5.1:+33199446 None:intergenic
ACCATCAGCAACATGAGCAA+AGG 0.634882 5.1:+33199580 None:intergenic
TTGTTCGAGTCAACCATCAA+CGG 0.637876 5.1:+33199989 None:intergenic
TTATACTAATTTAGAATCGG+CGG 0.638414 5.1:-33199747 MS.gene92914:CDS
AAATTAGTATAAACATGAGA+CGG 0.651862 5.1:+33199758 None:intergenic
ACACCATATACATTAAAAGG+AGG 0.660614 5.1:+33200433 None:intergenic
TATTTGATGACTTATTCGGT+CGG 0.668923 5.1:-33200460 None:intergenic
TAATACAGGGATGTTTCAAG+GGG 0.675676 5.1:-33200107 MS.gene92914:CDS
AGGGTTAGATGACAGCGAGG+AGG 0.678229 5.1:-33199822 MS.gene92914:CDS
TAAGCCTAGTGATTGTACCA+AGG 0.684085 5.1:-33199450 MS.gene92914:CDS
AGTGATCAATCATATTCACA+TGG 0.693931 5.1:-33200217 MS.gene92914:CDS
GATGTGGACACAATAATACA+GGG 0.698577 5.1:-33200120 MS.gene92914:CDS
TGGAGGGTTAGATGACAGCG+AGG 0.736906 5.1:-33199825 MS.gene92914:CDS
AAATGAAACAGAATGACCGG+TGG 0.751744 5.1:+33200159 None:intergenic
ACCGTCACCAGAAACCATAG+CGG 0.784680 5.1:+33199940 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTTATACTAATTTAGAAT+CGG - chr5.1:33200149-33200168 MS.gene92914:CDS 15.0%
!! AAATTAGTATAAACATGAGA+CGG + chr5.1:33200144-33200163 None:intergenic 20.0%
!!! GATTTTGAAGGATTAAATAT+AGG + chr5.1:33199562-33199581 None:intergenic 20.0%
! AACAAAAGAATAGAATTTGG+AGG - chr5.1:33200057-33200076 MS.gene92914:CDS 25.0%
! ACAAAAGAATAGAATTTGGA+GGG - chr5.1:33200058-33200077 MS.gene92914:CDS 25.0%
! ACAACACCATATACATTAAA+AGG + chr5.1:33199472-33199491 None:intergenic 25.0%
! GGAAACAAAAGAATAGAATT+TGG - chr5.1:33200054-33200073 MS.gene92914:CDS 25.0%
! TTAAAATGTGCAGTGATTAT+AGG + chr5.1:33200267-33200286 None:intergenic 25.0%
!! ATAATCACTGCACATTTTAA+AGG - chr5.1:33200267-33200286 MS.gene92914:CDS 25.0%
!! TTATACTAATTTAGAATCGG+CGG - chr5.1:33200152-33200171 MS.gene92914:CDS 25.0%
!!! TTGTAACTTGATGATTTTGA+AGG + chr5.1:33199574-33199593 None:intergenic 25.0%
AATAATACAGGGATGTTTCA+AGG - chr5.1:33199790-33199809 MS.gene92914:CDS 30.0%
AATTGTGTTGTAAGAGACAT+AGG + chr5.1:33199850-33199869 None:intergenic 30.0%
ACACAATTGGAATCTTCAAT+TGG - chr5.1:33199862-33199881 MS.gene92914:CDS 30.0%
ACACCATATACATTAAAAGG+AGG + chr5.1:33199469-33199488 None:intergenic 30.0%
AGTGATCAATCATATTCACA+TGG - chr5.1:33199682-33199701 MS.gene92914:CDS 30.0%
ATAATACAGGGATGTTTCAA+GGG - chr5.1:33199791-33199810 MS.gene92914:CDS 30.0%
ATACAGGTAGTGACATTATT+TGG - chr5.1:33199494-33199513 MS.gene92914:CDS 30.0%
CAAAGGTTCAATAGTTGATT+AGG + chr5.1:33200213-33200232 None:intergenic 30.0%
CTGATGTGATACTATAACAA+AGG + chr5.1:33200230-33200249 None:intergenic 30.0%
TATGTCTCTTACAACACAAT+TGG - chr5.1:33199849-33199868 MS.gene92914:CDS 30.0%
TCATTTCCTAAAACCGTTAT+CGG - chr5.1:33199757-33199776 MS.gene92914:CDS 30.0%
TGAAGGATTAAATATAGGAG+TGG + chr5.1:33199557-33199576 None:intergenic 30.0%
! ACACCTCCTTTTAATGTATA+TGG - chr5.1:33199463-33199482 MS.gene92914:CDS 30.0%
!! ACATTAGAAGCATTTAGTGT+TGG - chr5.1:33200033-33200052 MS.gene92914:CDS 30.0%
!!! TGAGAGTCTTTTTTCACAAA+AGG + chr5.1:33199997-33200016 None:intergenic 30.0%
AATTCCAAGACCAACTATAC+CGG + chr5.1:33199824-33199843 None:intergenic 35.0%
AGAGTTCTGTTCATTACAAG+AGG + chr5.1:33199641-33199660 None:intergenic 35.0%
AGGAAATGAAACAGAATGAC+CGG + chr5.1:33199746-33199765 None:intergenic 35.0%
CAATTGGAATCTTCAATTGG+TGG - chr5.1:33199865-33199884 MS.gene92914:CDS 35.0%
CTTGGCACAACTAAATTTGT+TGG - chr5.1:33200392-33200411 MS.gene92914:CDS 35.0%
GATGTGGACACAATAATACA+GGG - chr5.1:33199779-33199798 MS.gene92914:CDS 35.0%
GGTAACATCATACTTGATTC+AGG - chr5.1:33200099-33200118 MS.gene92914:CDS 35.0%
TAATACAGGGATGTTTCAAG+GGG - chr5.1:33199792-33199811 MS.gene92914:CDS 35.0%
TGACATAAGTTAGAAGAGCA+AGG + chr5.1:33199604-33199623 None:intergenic 35.0%
! AAGACGAGCAAACTCAATTT+TGG - chr5.1:33199931-33199950 MS.gene92914:CDS 35.0%
! GCTTTTACATCATCTCAAAC+TGG - chr5.1:33200354-33200373 MS.gene92914:CDS 35.0%
! TAGTGTTGAGACTCTTACAT+TGG - chr5.1:33199711-33199730 MS.gene92914:CDS 35.0%
!! ACATGAGCAAAGGTACTAAT+TGG + chr5.1:33200312-33200331 None:intergenic 35.0%
!! CATGAGCAAAGGTACTAATT+GGG + chr5.1:33200311-33200330 None:intergenic 35.0%
!! GCACAACTAAATTTGTTGGT+TGG - chr5.1:33200396-33200415 MS.gene92914:CDS 35.0%
!! GTATATGGTGTTGTTGATAC+AGG - chr5.1:33199478-33199497 MS.gene92914:CDS 35.0%
AAATGAAACAGAATGACCGG+TGG + chr5.1:33199743-33199762 None:intergenic 40.0%
ATTGTGTCCACATCCGATAA+CGG + chr5.1:33199773-33199792 None:intergenic 40.0%
GGATGTGGACACAATAATAC+AGG - chr5.1:33199778-33199797 MS.gene92914:CDS 40.0%
TAAGCCTAGTGATTGTACCA+AGG - chr5.1:33200449-33200468 MS.gene92914:CDS 40.0%
TACACCTTGGTACAATCACT+AGG + chr5.1:33200456-33200475 None:intergenic 40.0%
TGCTTGTAGCATTGTTCACA+AGG + chr5.1:33199532-33199551 None:intergenic 40.0%
TGTGCCAAGTTTCCAAAGAT+GGG + chr5.1:33200381-33200400 None:intergenic 40.0%
TTGTGCCAAGTTTCCAAAGA+TGG + chr5.1:33200382-33200401 None:intergenic 40.0%
TTGTTCGAGTCAACCATCAA+CGG + chr5.1:33199913-33199932 None:intergenic 40.0%
! TACTAATTTAGAATCGGCGG+TGG - chr5.1:33200155-33200174 MS.gene92914:CDS 40.0%
! TTACTGTTTGTTGCCGTTGA+TGG - chr5.1:33199897-33199916 MS.gene92914:CDS 40.0%
!! GGTATAGTTGGTCTTGGAAT+TGG - chr5.1:33199823-33199842 MS.gene92914:CDS 40.0%
ACCATCAGCAACATGAGCAA+AGG + chr5.1:33200322-33200341 None:intergenic 45.0%
ACCTTTGCTCATGTTGCTGA+TGG - chr5.1:33200318-33200337 MS.gene92914:CDS 45.0%
ATGTTTCAAGGGGCAACTTC+CGG - chr5.1:33199802-33199821 MS.gene92914:CDS 45.0%
CCTAAAACCGTTATCGGATG+TGG - chr5.1:33199763-33199782 MS.gene92914:CDS 45.0%
CTTACATTGGATTCCACCAC+CGG - chr5.1:33199724-33199743 MS.gene92914:CDS 45.0%
TCTCAAACTGGTCCCATCTT+TGG - chr5.1:33200366-33200385 MS.gene92914:CDS 45.0%
! ACTTCCGGTATAGTTGGTCT+TGG - chr5.1:33199817-33199836 MS.gene92914:CDS 45.0%
! TGGTCCCATCTTTGGAAACT+TGG - chr5.1:33200374-33200393 MS.gene92914:CDS 45.0%
!! CAATTTTGGAGATGCCGCTA+TGG - chr5.1:33199945-33199964 MS.gene92914:CDS 45.0%
!! CCACATCCGATAACGGTTTT+AGG + chr5.1:33199766-33199785 None:intergenic 45.0%
ACCGTCACCAGAAACCATAG+CGG + chr5.1:33199962-33199981 None:intergenic 50.0%
TGAAACAGAATGACCGGTGG+TGG + chr5.1:33199740-33199759 None:intergenic 50.0%
! TGCTCATGTTGCTGATGGTG+TGG - chr5.1:33200323-33200342 MS.gene92914:CDS 50.0%
AGGGTTAGATGACAGCGAGG+AGG - chr5.1:33200077-33200096 MS.gene92914:CDS 55.0%
GGAGATGCCGCTATGGTTTC+TGG - chr5.1:33199952-33199971 MS.gene92914:CDS 55.0%
GGGGCAACTTCCGGTATAGT+TGG - chr5.1:33199811-33199830 MS.gene92914:CDS 55.0%
GGGTTAGATGACAGCGAGGA+GGG - chr5.1:33200078-33200097 MS.gene92914:CDS 55.0%
TGGAGGGTTAGATGACAGCG+AGG - chr5.1:33200074-33200093 MS.gene92914:CDS 55.0%
!! GCCGCTATGGTTTCTGGTGA+CGG - chr5.1:33199958-33199977 MS.gene92914:CDS 55.0%
AGAATCGGCGGTGGCGCAAT+TGG - chr5.1:33200164-33200183 MS.gene92914:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 33199445 33200476 33199445 ID=MS.gene92914
chr5.1 mRNA 33199445 33200476 33199445 ID=MS.gene92914.t1;Parent=MS.gene92914
chr5.1 exon 33199445 33200476 33199445 ID=MS.gene92914.t1.exon1;Parent=MS.gene92914.t1
chr5.1 CDS 33199445 33200476 33199445 ID=cds.MS.gene92914.t1;Parent=MS.gene92914.t1
Gene Sequence

>MS.gene92914

ATGACTTATTCGGTCGGTACACCTCCTTTTAATGTATATGGTGTTGTTGATACAGGTAGTGACATTATTTGGCTTCAATGTAAGCCTTGTGAACAATGCTACAAGCAAACCACTCCTATATTTAATCCTTCAAAATCATCAAGTTACAAAAATATTCCTTGCTCTTCTAACTTATGTCAATCTGTGAGATATACCTCTTGTAATGAACAGAACTCTTGTGAATATACTATTAACTTTAGTGATCAATCATATTCACATGGAGAACTTAGTGTTGAGACTCTTACATTGGATTCCACCACCGGTCATTCTGTTTCATTTCCTAAAACCGTTATCGGATGTGGACACAATAATACAGGGATGTTTCAAGGGGCAACTTCCGGTATAGTTGGTCTTGGAATTGGACCTATGTCTCTTACAACACAATTGGAATCTTCAATTGGTGGTAAATTTTCTTACTGTTTGTTGCCGTTGATGGTTGACTCGAACAAGACGAGCAAACTCAATTTTGGAGATGCCGCTATGGTTTCTGGTGACGGTGTTGTGTCAACTCCTTTTGTGAAAAAAGACTCTCAAGTTTTCTACTATTTGACATTAGAAGCATTTAGTGTTGGAAACAAAAGAATAGAATTTGGAGGGTTAGATGACAGCGAGGAGGGTAACATCATACTTGATTCAGGTACAACATTGACTCTTTTACCGTCTCATGTTTATACTAATTTAGAATCGGCGGTGGCGCAATTGGTGAAACTAGATCGTGTTGATGATCCTAATCAACTATTGAACCTTTGTTATAGTATCACATCAGATCAATATGATTTTCCTATAATCACTGCACATTTTAAAGGTGCAGATATTAAGTTGAACCCAATTAGTACCTTTGCTCATGTTGCTGATGGTGTGGTTTGCTTAGCTTTTACATCATCTCAAACTGGTCCCATCTTTGGAAACTTGGCACAACTAAATTTGTTGGTTGGTTATGATCTTCAACAAAATATTGTGTCGTTTAAGCCTAGTGATTGTACCAAGGTGTAA
Protein sequence

>MS.gene92914.t1

MTYSVGTPPFNVYGVVDTGSDIIWLQCKPCEQCYKQTTPIFNPSKSSSYKNIPCSSNLCQSVRYTSCNEQNSCEYTINFSDQSYSHGELSVETLTLDSTTGHSVSFPKTVIGCGHNNTGMFQGATSGIVGLGIGPMSLTTQLESSIGGKFSYCLLPLMVDSNKTSKLNFGDAAMVSGDGVVSTPFVKKDSQVFYYLTLEAFSVGNKRIEFGGLDDSEEGNIILDSGTTLTLLPSHVYTNLESAVAQLVKLDRVDDPNQLLNLCYSITSDQYDFPIITAHFKGADIKLNPISTFAHVADGVVCLAFTSSQTGPIFGNLAQLNLLVGYDLQQNIVSFKPSDCTKV