AlfalfaGEDB | MS.gene92955

M. sativa cultivar XinJiangDaYe / MS.gene92955

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene92955.t1	XP_013442640.1	84.6	214	33	0	1	214	1	214	8.30E-97	363.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene92955.t1	Q9FKK2	52.7	167	76	2	5	168	3	169	8.8e-39	161.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene92955.t1	A0A072TSY1	84.6	214	33	0	1	214	1	214	6.0e-97	363.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene92955.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene92955	MS.gene22094	PPI
MS.gene92955	MS.gene068836	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene92955.t1	MTR_0121s0080	84.186	215	34	0	1	215	1	215	8.57e-133	373
MS.gene92955.t1	MTR_7g062350	83.333	192	32	0	24	215	36	227	1.45e-115	330
MS.gene92955.t1	MTR_2g030740	72.558	215	59	0	1	215	1	215	1.19e-112	322
MS.gene92955.t1	MTR_1g105920	72.093	215	60	0	1	215	1	215	3.86e-112	320
MS.gene92955.t1	MTR_8g043650	71.628	215	61	0	1	215	1	215	3.72e-111	318
MS.gene92955.t1	MTR_1g012570	69.302	215	66	0	1	215	1	215	1.90e-107	309
MS.gene92955.t1	MTR_1g105910	69.767	215	65	0	1	215	1	215	2.17e-107	308
MS.gene92955.t1	MTR_3g052920	68.837	215	67	0	1	215	1	215	4.39e-106	305
MS.gene92955.t1	MTR_1g108510	69.124	217	59	1	1	217	1	209	7.51e-106	304
MS.gene92955.t1	MTR_2g093190	70.046	217	57	1	1	217	1	209	1.32e-104	301
MS.gene92955.t1	MTR_3g030770	69.124	217	59	1	1	217	1	209	1.61e-104	301
MS.gene92955.t1	MTR_1g108580	69.585	217	58	1	1	217	1	209	2.36e-104	300
MS.gene92955.t1	MTR_1g075600	61.860	215	82	0	1	215	1	215	1.19e-93	274
MS.gene92955.t1	MTR_1g106070	71.823	181	51	0	1	181	1	181	3.95e-93	270
MS.gene92955.t1	MTR_1g077360	60.930	215	84	0	1	215	1	215	1.27e-92	271
MS.gene92955.t1	MTR_2g085250	60.000	215	86	0	1	215	1	215	4.09e-90	265
MS.gene92955.t1	MTR_3g052870	63.256	215	60	2	1	215	1	196	2.20e-89	262
MS.gene92955.t1	MTR_2g085280	59.535	215	87	0	1	215	1	215	2.96e-89	263
MS.gene92955.t1	MTR_5g053390	60.930	215	84	0	1	215	1	215	3.23e-89	263
MS.gene92955.t1	MTR_5g045560	60.930	215	84	0	1	215	1	215	3.41e-89	262
MS.gene92955.t1	MTR_1g105905	63.959	197	71	0	18	214	1	197	5.03e-88	259
MS.gene92955.t1	MTR_5g055100	58.605	215	89	0	1	215	1	215	1.15e-87	258
MS.gene92955.t1	MTR_3g030780	85.606	132	19	0	1	132	1	132	3.58e-78	231
MS.gene92955.t1	MTR_0121s0100	71.970	132	37	0	1	132	1	132	2.27e-62	191
MS.gene92955.t1	MTR_1g075570	55.056	178	66	1	18	195	1	164	2.22e-54	171
MS.gene92955.t1	MTR_3g080940	43.523	193	103	1	9	195	9	201	4.12e-52	167
MS.gene92955.t1	MTR_3g067910	61.000	100	39	0	23	122	4	103	1.87e-37	126
MS.gene92955.t1	MTR_1g108500	72.917	96	5	1	23	118	111	185	5.31e-37	132
MS.gene92955.t1	MTR_8g086290	38.462	169	94	2	5	170	8	169	1.62e-31	113
MS.gene92955.t1	MTR_2g105290	36.747	166	95	2	5	167	8	166	1.09e-30	111
MS.gene92955.t1	MTR_7g028448	41.606	137	68	3	23	159	26	150	6.61e-25	96.3
MS.gene92955.t1	MTR_4g036915	41.606	137	68	3	23	159	26	150	6.61e-25	96.3
MS.gene92955.t1	MTR_3g067875	75.000	56	14	0	18	73	1	56	2.20e-23	89.4
MS.gene92955.t1	MTR_3g067915	62.687	67	25	0	60	126	30	96	2.47e-23	90.1
MS.gene92955.t1	MTR_4g127140	34.104	173	100	3	5	167	8	176	7.80e-22	89.0
MS.gene92955.t1	MTR_1g047550	46.667	90	39	1	9	89	2	91	1.45e-20	85.5
MS.gene92955.t1	MTR_4g131030	36.719	128	66	2	9	121	2	129	1.58e-20	86.3
MS.gene92955.t1	MTR_1g054265	40.708	113	65	1	9	121	2	112	3.58e-20	84.0
MS.gene92955.t1	MTR_8g079502	53.623	69	32	0	9	77	2	70	5.70e-20	84.0
MS.gene92955.t1	MTR_3g067870	61.905	63	24	0	16	78	83	145	2.72e-19	81.3
MS.gene92955.t1	MTR_1g063160	41.837	98	55	2	9	106	2	97	8.10e-19	80.9
MS.gene92955.t1	MTR_8g051580	50.725	69	34	0	9	77	2	70	2.51e-18	79.3
MS.gene92955.t1	MTR_4g094632	39.091	110	65	1	9	118	2	109	2.65e-18	79.0
MS.gene92955.t1	MTR_8g046350	49.275	69	35	0	9	77	2	70	1.17e-17	77.4
MS.gene92955.t1	MTR_4g094638	37.736	106	65	1	9	113	2	107	5.42e-15	70.5
MS.gene92955.t1	MTR_3g005530	45.588	68	37	0	9	76	2	69	5.50e-15	71.2
MS.gene92955.t1	MTR_8g033270	47.143	70	35	1	7	76	2	69	1.49e-14	70.9
MS.gene92955.t1	MTR_4g093970	48.485	66	34	0	10	75	29	94	2.09e-14	70.1
MS.gene92955.t1	MTR_3g452380	44.595	74	41	0	3	76	11	84	3.12e-14	69.7
MS.gene92955.t1	MTR_6g464720	36.000	125	70	5	7	126	2	121	3.46e-14	69.7
MS.gene92955.t1	MTR_8g022970	31.013	158	96	3	8	159	17	167	3.56e-14	68.6
MS.gene92955.t1	MTR_4g102530	42.466	73	42	0	10	82	3	75	5.46e-14	68.6
MS.gene92955.t1	MTR_4g109830	45.070	71	36	2	7	76	2	70	6.14e-14	68.6
MS.gene92955.t1	MTR_5g066180	44.118	68	38	0	9	76	2	69	8.18e-14	68.2
MS.gene92955.t1	MTR_1g053070	36.634	101	63	1	9	108	2	102	8.53e-14	68.6
MS.gene92955.t1	MTR_4g084740	41.772	79	44	2	9	86	2	79	1.32e-13	68.9
MS.gene92955.t1	MTR_3g102570	39.216	102	59	3	9	109	2	101	1.51e-13	68.6
MS.gene92955.t1	MTR_8g087860	44.118	68	38	0	9	76	18	85	4.32e-13	66.6
MS.gene92955.t1	MTR_0003s0590	37.805	82	51	0	9	90	2	83	4.56e-13	66.6
MS.gene92955.t1	MTR_6g015975	39.785	93	50	3	7	95	2	92	5.48e-13	66.2
MS.gene92955.t1	MTR_2g017865	44.118	68	38	0	9	76	17	84	7.43e-13	66.2
MS.gene92955.t1	MTR_2g009890	41.176	68	40	0	9	76	2	69	9.32e-13	66.2
MS.gene92955.t1	MTR_1g101970	42.647	68	39	0	9	76	2	69	9.47e-13	64.7
MS.gene92955.t1	MTR_3g084980	36.364	110	64	3	7	112	2	109	9.60e-13	65.5
MS.gene92955.t1	MTR_7g016600	45.070	71	36	2	7	76	2	70	9.75e-13	65.5
MS.gene92955.t1	MTR_5g032150	41.176	68	40	0	9	76	2	69	1.04e-12	65.5
MS.gene92955.t1	MTR_5g032520	41.176	68	40	0	9	76	2	69	1.05e-12	65.1
MS.gene92955.t1	MTR_8g097090	37.255	102	58	3	7	104	2	101	1.13e-12	65.5
MS.gene92955.t1	MTR_8g033220	40.476	84	49	1	10	92	3	86	1.37e-12	64.7
MS.gene92955.t1	MTR_8g066260	43.662	71	37	2	7	76	2	70	1.38e-12	65.1
MS.gene92955.t1	MTR_8g033220	40.476	84	49	1	10	92	3	86	1.62e-12	64.7
MS.gene92955.t1	MTR_7g075870	43.284	67	38	0	10	76	3	69	2.06e-12	64.3
MS.gene92955.t1	MTR_2g461710	51.724	58	26	1	7	64	2	57	2.51e-12	60.5
MS.gene92955.t1	MTR_7g016630	34.259	108	61	3	7	106	2	107	6.75e-12	63.5
MS.gene92955.t1	MTR_5g031000	41.176	68	40	0	9	76	2	69	6.82e-12	63.2
MS.gene92955.t1	MTR_5g031000	41.176	68	40	0	9	76	2	69	7.33e-12	63.2
MS.gene92955.t1	MTR_5g046870	40.580	69	40	1	9	76	2	70	3.04e-11	58.9
MS.gene92955.t1	MTR_7g075870	42.647	68	38	1	10	76	3	70	3.17e-11	60.8
MS.gene92955.t1	MTR_5g021270	42.857	70	38	1	9	76	2	71	3.85e-11	60.8
MS.gene92955.t1	MTR_5g046790	37.681	69	42	1	9	76	2	70	3.88e-11	60.8
MS.gene92955.t1	MTR_5g021270	42.857	70	38	1	9	76	2	71	4.17e-11	60.8
MS.gene92955.t1	MTR_3g088615	28.346	127	72	2	9	133	2	111	6.23e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene92955.t1	AT5G60440	52.695	167	76	2	5	168	3	169	3.84e-50	165
MS.gene92955.t1	AT2G24840	41.975	162	81	3	9	163	63	218	2.98e-35	125
MS.gene92955.t1	AT1G65360	37.566	189	111	3	5	192	3	185	1.12e-33	120
MS.gene92955.t1	AT1G01530	39.899	198	104	6	4	190	2	195	1.74e-33	120
MS.gene92955.t1	AT4G36590	36.264	182	108	3	5	179	3	183	2.75e-28	107
MS.gene92955.t1	AT3G04100	28.358	201	130	4	2	193	7	202	5.30e-23	92.4
MS.gene92955.t1	AT2G34440	39.823	113	67	1	9	121	2	113	1.49e-20	85.1
MS.gene92955.t1	AT3G66656	37.815	119	70	1	9	123	2	120	1.72e-20	85.1
MS.gene92955.t1	AT1G28460	29.240	171	114	2	5	168	4	174	6.14e-18	78.6
MS.gene92955.t1	AT1G72350	49.315	73	35	1	9	81	43	113	1.02e-17	79.0
MS.gene92955.t1	AT4G09960	37.190	121	70	3	9	126	2	119	8.34e-16	73.6
MS.gene92955.t1	AT2G45650	48.571	70	34	1	7	76	2	69	9.64e-16	73.9
MS.gene92955.t1	AT4G09960	37.600	125	70	4	5	126	26	145	1.21e-15	73.9
MS.gene92955.t1	AT4G09960	37.190	121	70	3	9	126	2	119	1.38e-15	73.2
MS.gene92955.t1	AT4G09960	37.190	121	70	3	9	126	3	120	1.52e-15	73.2
MS.gene92955.t1	AT4G09960	37.190	121	70	3	9	126	12	129	1.70e-15	73.2
MS.gene92955.t1	AT1G29962	36.634	101	61	1	5	102	4	104	2.13e-15	72.0
MS.gene92955.t1	AT4G09960	37.600	125	70	4	5	126	95	214	2.54e-15	73.9
MS.gene92955.t1	AT2G45660	45.588	68	37	0	10	77	3	70	6.54e-15	69.7
MS.gene92955.t1	AT2G45660	45.588	68	37	0	10	77	3	70	1.45e-14	70.1
MS.gene92955.t1	AT4G22950	46.269	67	36	0	10	76	3	69	1.97e-14	68.9
MS.gene92955.t1	AT1G28450	27.586	174	116	3	5	168	4	177	2.42e-14	68.9
MS.gene92955.t1	AT1G22130	36.957	92	57	1	9	99	2	93	4.59e-14	70.5
MS.gene92955.t1	AT4G22950	46.269	67	36	0	10	76	3	69	5.35e-14	68.6
MS.gene92955.t1	AT4G22950	46.269	67	36	0	10	76	3	69	5.35e-14	68.6
MS.gene92955.t1	AT1G77950	41.026	78	44	2	9	85	2	78	6.99e-14	68.9
MS.gene92955.t1	AT1G77950	41.026	78	44	2	9	85	2	78	6.99e-14	68.9
MS.gene92955.t1	AT1G77950	41.026	78	44	2	9	85	2	78	6.99e-14	68.9
MS.gene92955.t1	AT1G77950	41.026	78	44	2	9	85	2	78	6.99e-14	68.9
MS.gene92955.t1	AT1G77980	38.636	88	53	1	9	95	2	89	7.41e-14	69.7
MS.gene92955.t1	AT3G57390	45.714	70	36	1	7	76	2	69	1.22e-13	67.8
MS.gene92955.t1	AT5G51870	47.761	67	35	0	10	76	3	69	1.31e-13	66.6
MS.gene92955.t1	AT5G51870	47.761	67	35	0	10	76	3	69	1.33e-13	67.4
MS.gene92955.t1	AT2G42830	44.000	75	40	1	2	76	12	84	1.37e-13	68.2
MS.gene92955.t1	AT2G42830	44.000	75	40	1	2	76	12	84	1.39e-13	68.2
MS.gene92955.t1	AT2G22540	44.118	68	38	0	9	76	2	69	1.52e-13	67.8
MS.gene92955.t1	AT2G22540	44.118	68	38	0	9	76	2	69	1.52e-13	67.8
MS.gene92955.t1	AT3G57230	39.706	68	41	0	9	76	2	69	1.60e-13	65.9
MS.gene92955.t1	AT3G58780	44.000	75	40	1	2	76	12	84	1.91e-13	67.8
MS.gene92955.t1	AT5G51870	47.761	67	35	0	10	76	3	69	2.14e-13	67.0
MS.gene92955.t1	AT3G57390	45.714	70	36	1	7	76	2	69	2.20e-13	67.8
MS.gene92955.t1	AT3G58780	44.000	75	40	1	2	76	18	90	2.21e-13	67.4
MS.gene92955.t1	AT5G13790	45.588	68	37	0	9	76	2	69	2.46e-13	67.8
MS.gene92955.t1	AT5G51870	47.761	67	35	0	10	76	13	79	2.51e-13	67.0
MS.gene92955.t1	AT5G13790	45.588	68	37	0	9	76	2	69	2.56e-13	67.8
MS.gene92955.t1	AT4G18960	35.000	100	55	1	9	108	18	107	2.59e-13	67.4
MS.gene92955.t1	AT5G51870	47.761	67	35	0	10	76	26	92	2.75e-13	66.2
MS.gene92955.t1	AT3G58780	44.000	75	40	1	2	76	12	84	2.78e-13	67.8
MS.gene92955.t1	AT4G24540	44.118	68	38	0	9	76	2	69	3.42e-13	66.6
MS.gene92955.t1	AT1G17310	50.000	74	35	1	8	81	47	118	3.74e-13	66.2
MS.gene92955.t1	AT4G37940	39.506	81	42	1	9	89	2	75	3.88e-13	66.2
MS.gene92955.t1	AT4G37940	39.506	81	42	1	9	89	2	75	3.90e-13	66.6
MS.gene92955.t1	AT4G37940	39.506	81	42	1	9	89	2	75	4.81e-13	65.1
MS.gene92955.t1	AT1G26310	45.070	71	36	2	7	76	2	70	5.12e-13	66.6
MS.gene92955.t1	AT5G10140	35.294	102	61	2	9	109	2	99	5.24e-13	65.1
MS.gene92955.t1	AT3G30260	43.662	71	37	2	7	76	2	70	5.40e-13	66.2
MS.gene92955.t1	AT1G17310	50.000	74	35	1	8	81	58	129	5.43e-13	66.2
MS.gene92955.t1	AT1G69120	45.070	71	36	2	7	76	2	70	6.29e-13	65.9
MS.gene92955.t1	AT3G57230	39.706	68	41	0	9	76	2	69	7.86e-13	65.9
MS.gene92955.t1	AT3G57230	39.706	68	41	0	9	76	2	69	7.86e-13	65.9
MS.gene92955.t1	AT5G10140	35.294	102	61	2	9	109	2	99	8.12e-13	64.7
MS.gene92955.t1	AT5G60910	45.070	71	36	2	7	76	2	70	8.98e-13	65.9
MS.gene92955.t1	AT5G10140	34.286	105	64	2	9	112	2	102	9.83e-13	64.7
MS.gene92955.t1	AT5G10140	35.294	102	61	2	9	109	2	99	9.95e-13	64.7
MS.gene92955.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.26e-12	63.9
MS.gene92955.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.26e-12	63.9
MS.gene92955.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.26e-12	63.9
MS.gene92955.t1	AT1G69120	45.070	71	36	2	7	76	2	70	1.42e-12	65.5
MS.gene92955.t1	AT5G20240	33.333	129	63	4	9	133	2	111	1.44e-12	63.5
MS.gene92955.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.51e-12	63.5
MS.gene92955.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.51e-12	63.5
MS.gene92955.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.51e-12	63.5
MS.gene92955.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.51e-12	63.5
MS.gene92955.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.51e-12	63.5
MS.gene92955.t1	AT5G15800	38.298	94	51	3	7	95	2	93	1.67e-12	65.1
MS.gene92955.t1	AT5G20240	33.333	129	63	4	9	133	2	111	1.76e-12	64.3
MS.gene92955.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.76e-12	62.0
MS.gene92955.t1	AT3G61120	42.647	68	39	0	9	76	2	69	2.37e-12	64.7
MS.gene92955.t1	AT5G15800	38.298	94	51	3	7	95	2	93	2.71e-12	64.7
MS.gene92955.t1	AT5G51860	44.776	67	37	0	10	76	3	69	3.60e-12	63.5
MS.gene92955.t1	AT5G62165	41.791	67	39	0	10	76	3	69	4.17e-12	63.5
MS.gene92955.t1	AT5G62165	41.791	67	39	0	10	76	3	69	4.17e-12	63.5
MS.gene92955.t1	AT5G62165	41.791	67	39	0	10	76	3	69	4.17e-12	63.5
MS.gene92955.t1	AT5G62165	41.791	67	39	0	10	76	3	69	4.28e-12	63.2
MS.gene92955.t1	AT5G62165	41.791	67	39	0	10	76	3	69	4.28e-12	63.2
MS.gene92955.t1	AT5G51860	44.776	67	37	0	10	76	3	69	4.63e-12	63.2
MS.gene92955.t1	AT2G22630	39.706	68	41	0	9	76	2	69	8.08e-12	62.8
MS.gene92955.t1	AT2G22630	39.706	68	41	0	9	76	2	69	8.08e-12	62.8
MS.gene92955.t1	AT3G54340	41.429	70	39	1	9	76	2	71	8.84e-12	62.8
MS.gene92955.t1	AT3G02310	37.234	94	52	3	7	95	2	93	1.38e-11	61.6
MS.gene92955.t1	AT4G11880	42.647	68	38	1	10	76	3	70	1.65e-11	61.2
MS.gene92955.t1	AT4G11880	42.647	68	38	1	10	76	3	70	1.65e-11	61.2
MS.gene92955.t1	AT1G24260	36.735	98	56	3	7	100	2	97	2.48e-11	61.6
MS.gene92955.t1	AT5G49420	35.106	94	54	2	5	91	3	96	2.56e-11	62.4
MS.gene92955.t1	AT1G47760	37.313	67	42	0	9	75	2	68	3.08e-11	60.5
MS.gene92955.t1	AT4G11880	42.647	68	38	1	10	76	3	70	3.09e-11	61.2
MS.gene92955.t1	AT4G11880	42.647	68	38	1	10	76	3	70	3.09e-11	61.2
MS.gene92955.t1	AT1G24260	36.458	96	55	3	7	98	2	95	3.10e-11	61.2
MS.gene92955.t1	AT1G24260	36.458	96	55	3	7	98	2	95	3.26e-11	61.6
MS.gene92955.t1	AT3G02310	37.234	94	52	3	7	95	2	93	3.56e-11	61.2
MS.gene92955.t1	AT5G65050	40.580	69	40	1	9	76	2	70	7.45e-11	59.3

Find 40 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAATTATTCAAGAAGATTT+TGG	0.197704	1.2:+80650442	MS.gene92955:CDS
TGTTGATAGGAGTGCAATTC+AGG	0.259070	1.2:+80650493	MS.gene92955:CDS
AAATCATTACCAACAGAAAA+TGG	0.265409	1.2:-80650536	None:intergenic
TGGTGGGCTTGTCCGATTAA+TGG	0.300675	1.2:+80650401	MS.gene92955:CDS
AGGCGTTCAAGGCTCAATTT+TGG	0.334747	1.2:+80650381	MS.gene92955:CDS
GTTGAGGATTTGGCTGATAA+TGG	0.350346	1.2:-80650577	None:intergenic
GGTGGGCTTGTCCGATTAAT+GGG	0.369852	1.2:+80650402	MS.gene92955:CDS
CCTTGAACGCCTTGCACAAA+AGG	0.373825	1.2:-80650370	None:intergenic
GTCCGATTAATGGGATGAAT+AGG	0.377904	1.2:+80650411	MS.gene92955:CDS
GCCCTATTCATCCCATTAAT+CGG	0.382765	1.2:-80650413	None:intergenic
TCTATTACCGTATCAACATT+TGG	0.384428	1.2:-80650197	None:intergenic
GTAATAGATCGCTATCTCTC+TGG	0.394171	1.2:+80650212	MS.gene92955:CDS
TTGTTGCTCATCATGTTGAT+AGG	0.402553	1.2:+80650480	MS.gene92955:CDS
TCCGATTAATGGGATGAATA+GGG	0.426477	1.2:+80650412	MS.gene92955:CDS
TAATAGATCGCTATCTCTCT+GGG	0.445296	1.2:+80650213	MS.gene92955:CDS
ATAATGGAGAGGGATGTTAG+AGG	0.448969	1.2:-80650561	None:intergenic
ATTAAATGAGGTTGAACATA+GGG	0.483578	1.2:-80650634	None:intergenic
ACCTTTCCTTCCGCTTGACA+TGG	0.495891	1.2:-80649997	None:intergenic
CGGTCATCCAAATGTTGATA+CGG	0.500939	1.2:+80650190	MS.gene92955:CDS
CACTTGAAGGTTCCTCTCGT+TGG	0.501301	1.2:-80650054	None:intergenic
TGTATTCTCACCTAGTGAAA+AGG	0.518205	1.2:+80650157	MS.gene92955:CDS
AGCATTGAGCTCATACACTT+TGG	0.530404	1.2:-80650288	None:intergenic
GCTCATACACTTTGGCGCTA+CGG	0.535806	1.2:-80650280	None:intergenic
GTTGATAGGAGTGCAATTCA+GGG	0.537321	1.2:+80650494	MS.gene92955:CDS
TTGTTGAAGCCAATTAAATG+AGG	0.551491	1.2:-80650646	None:intergenic
AATTAAATGAGGTTGAACAT+AGG	0.556548	1.2:-80650635	None:intergenic
TGCACTTCAACCATGTCAAG+CGG	0.580198	1.2:+80649987	None:intergenic
ACTCAACAACACACTGGACG+AGG	0.585246	1.2:+80650322	MS.gene92955:CDS
ACCATGTCAAGCGGAAGGAA+AGG	0.588345	1.2:+80649996	None:intergenic
GTGAGCCTCAATGAATTCCA+TGG	0.607397	1.2:-80650258	None:intergenic
AGCAACATCTACACCACAGA+GGG	0.623582	1.2:-80650129	None:intergenic
CTTCAACCATGTCAAGCGGA+AGG	0.624189	1.2:+80649991	None:intergenic
TCAAGCGGAAGGAAAGGTCG+TGG	0.632671	1.2:+80650002	MS.gene92955:CDS
GGATTTGGCTGATAATGGAG+AGG	0.633381	1.2:-80650572	None:intergenic
AAGCACCATGGAATTCATTG+AGG	0.665445	1.2:+80650253	MS.gene92955:CDS
ACCACAAAACAAAAGCACCA+TGG	0.677029	1.2:+80650241	MS.gene92955:CDS
GATTTGGCTGATAATGGAGA+GGG	0.685158	1.2:-80650571	None:intergenic
GATTCAACTCAACAACACAC+TGG	0.690388	1.2:+80650316	MS.gene92955:CDS
AATAGATCGCTATCTCTCTG+GGG	0.733972	1.2:+80650214	MS.gene92955:CDS
GAGCAACATCTACACCACAG+AGG	0.777707	1.2:-80650130	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGAATTATTCAAGAAGATTT+TGG	+	chr1.2:80650442-80650461	MS.gene92955:CDS	20.0%
!	AAATCATTACCAACAGAAAA+TGG	-	chr1.2:80650539-80650558	None:intergenic	25.0%
!	ATAGGGTTATGAAAAATTTG+TGG	-	chr1.2:80650620-80650639	None:intergenic	25.0%
!!	AAAGAAAATACCTTTTCACT+AGG	-	chr1.2:80650170-80650189	None:intergenic	25.0%
!!	ATTATTCAAGAAGATTTTGG+TGG	+	chr1.2:80650445-80650464	MS.gene92955:CDS	25.0%
!!	GAAAATATCTCATTTTGTTG+AGG	-	chr1.2:80650596-80650615	None:intergenic	25.0%
!!!	AGTGAAAAGGTATTTTCTTT+CGG	+	chr1.2:80650170-80650189	MS.gene92955:CDS	25.0%
!!!	TCTTTTTCATCTCGATATTT+TGG	-	chr1.2:80650031-80650050	None:intergenic	25.0%
	TCTATTACCGTATCAACATT+TGG	-	chr1.2:80650200-80650219	None:intergenic	30.0%
!	ATCTCATTTTGTTGAGGATT+TGG	-	chr1.2:80650590-80650609	None:intergenic	30.0%
!	CAAACTTTTCCATTTTCTGT+TGG	+	chr1.2:80650527-80650546	MS.gene92955:CDS	30.0%
!	TCTTTGAAAAAGTCACTTGA+AGG	-	chr1.2:80650070-80650089	None:intergenic	30.0%
	TAATAGATCGCTATCTCTCT+GGG	+	chr1.2:80650213-80650232	MS.gene92955:CDS	35.0%
	TCCGATTAATGGGATGAATA+GGG	+	chr1.2:80650412-80650431	MS.gene92955:CDS	35.0%
	TGTATTCTCACCTAGTGAAA+AGG	+	chr1.2:80650157-80650176	MS.gene92955:CDS	35.0%
	TTGTTGCTCATCATGTTGAT+AGG	+	chr1.2:80650480-80650499	MS.gene92955:CDS	35.0%
!!!	ACTTTTTCAAAGAGTCGTAG+CGG	+	chr1.2:80650077-80650096	MS.gene92955:CDS	35.0%
	AAGCACCATGGAATTCATTG+AGG	+	chr1.2:80650253-80650272	MS.gene92955:CDS	40.0%
	AATAGATCGCTATCTCTCTG+GGG	+	chr1.2:80650214-80650233	MS.gene92955:CDS	40.0%
	CGGTCATCCAAATGTTGATA+CGG	+	chr1.2:80650190-80650209	MS.gene92955:CDS	40.0%
	GATTCAACTCAACAACACAC+TGG	+	chr1.2:80650316-80650335	MS.gene92955:CDS	40.0%
	GCCCTATTCATCCCATTAAT+CGG	-	chr1.2:80650416-80650435	None:intergenic	40.0%
	GTAATAGATCGCTATCTCTC+TGG	+	chr1.2:80650212-80650231	MS.gene92955:CDS	40.0%
	GTCCGATTAATGGGATGAAT+AGG	+	chr1.2:80650411-80650430	MS.gene92955:CDS	40.0%
	GTTGAGGATTTGGCTGATAA+TGG	-	chr1.2:80650580-80650599	None:intergenic	40.0%
	TGAAAAAGATGACCAACGAG+AGG	+	chr1.2:80650042-80650061	MS.gene92955:CDS	40.0%
!	AGCATTGAGCTCATACACTT+TGG	-	chr1.2:80650291-80650310	None:intergenic	40.0%
!	ATAATGGAGAGGGATGTTAG+AGG	-	chr1.2:80650564-80650583	None:intergenic	40.0%
!	GTTGATAGGAGTGCAATTCA+GGG	+	chr1.2:80650494-80650513	MS.gene92955:CDS	40.0%
!	TGTTGATAGGAGTGCAATTC+AGG	+	chr1.2:80650493-80650512	MS.gene92955:CDS	40.0%
!!	ACCACAAAACAAAAGCACCA+TGG	+	chr1.2:80650241-80650260	MS.gene92955:CDS	40.0%
!!	GATTTGGCTGATAATGGAGA+GGG	-	chr1.2:80650574-80650593	None:intergenic	40.0%
!!	GTTCAAGGCTCAATTTTGGT+GGG	+	chr1.2:80650385-80650404	MS.gene92955:CDS	40.0%
!!!	ATGGTGCTTTTGTTTTGTGG+TGG	-	chr1.2:80650242-80650261	None:intergenic	40.0%
!!!	CTTTTTCAAAGAGTCGTAGC+GGG	+	chr1.2:80650078-80650097	MS.gene92955:CDS	40.0%
!!!	TCCATGGTGCTTTTGTTTTG+TGG	-	chr1.2:80650245-80650264	None:intergenic	40.0%
!!!	TGGTGCTTTTGTTTTGTGGT+GGG	-	chr1.2:80650241-80650260	None:intergenic	40.0%
	AGCAACATCTACACCACAGA+GGG	-	chr1.2:80650132-80650151	None:intergenic	45.0%
	GTGAGCCTCAATGAATTCCA+TGG	-	chr1.2:80650261-80650280	None:intergenic	45.0%
!	AGGCGTTCAAGGCTCAATTT+TGG	+	chr1.2:80650381-80650400	MS.gene92955:CDS	45.0%
!	CGTTCAAGGCTCAATTTTGG+TGG	+	chr1.2:80650384-80650403	MS.gene92955:CDS	45.0%
!!	GGATTTGGCTGATAATGGAG+AGG	-	chr1.2:80650575-80650594	None:intergenic	45.0%
!!	TGAGTTGAGCCTTTTGTGCA+AGG	+	chr1.2:80650361-80650380	MS.gene92955:CDS	45.0%
	ACTCAACAACACACTGGACG+AGG	+	chr1.2:80650322-80650341	MS.gene92955:CDS	50.0%
	CACTTGAAGGTTCCTCTCGT+TGG	-	chr1.2:80650057-80650076	None:intergenic	50.0%
	CCTTGAACGCCTTGCACAAA+AGG	-	chr1.2:80650373-80650392	None:intergenic	50.0%
	CTGGACGAGGAGAAAAAGCA+CGG	+	chr1.2:80650335-80650354	MS.gene92955:CDS	50.0%
	GAGCAACATCTACACCACAG+AGG	-	chr1.2:80650133-80650152	None:intergenic	50.0%
	GCTCATACACTTTGGCGCTA+CGG	-	chr1.2:80650283-80650302	None:intergenic	50.0%
!	AGTGAGTTTTGCACCCTCTG+TGG	+	chr1.2:80650116-80650135	MS.gene92955:CDS	50.0%
!	GGTGGGCTTGTCCGATTAAT+GGG	+	chr1.2:80650402-80650421	MS.gene92955:CDS	50.0%
!!	CCTTTTGTGCAAGGCGTTCA+AGG	+	chr1.2:80650370-80650389	MS.gene92955:CDS	50.0%
!!	TGGTGGGCTTGTCCGATTAA+TGG	+	chr1.2:80650401-80650420	MS.gene92955:CDS	50.0%
	TCAAGCGGAAGGAAAGGTCG+TGG	+	chr1.2:80650002-80650021	MS.gene92955:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	80649999	80650655	80649999	ID=MS.gene92955
chr1.2	mRNA	80649999	80650655	80649999	ID=MS.gene92955.t1;Parent=MS.gene92955
chr1.2	exon	80649999	80650655	80649999	ID=MS.gene92955.t1.exon1;Parent=MS.gene92955.t1
chr1.2	CDS	80649999	80650655	80649999	ID=cds.MS.gene92955.t1;Parent=MS.gene92955.t1

Gene Sequence

>MS.gene92955

ATGTCAAGCGGAAGGAAAGGTCGTGGTCGCCAAAATATCGAGATGAAAAAGATGACCAACGAGAGGAACCTTCAAGTGACTTTTTCAAAGAGTCGTAGCGGGCTCTTCAAGAAATTCAGTGAGTTTTGCACCCTCTGTGGTGTAGATGTTGCTCTCGTTGTATTCTCACCTAGTGAAAAGGTATTTTCTTTCGGTCATCCAAATGTTGATACGGTAATAGATCGCTATCTCTCTGGGGTCCCACCACAAAACAAAAGCACCATGGAATTCATTGAGGCTCACCGTAGCGCCAAAGTGTATGAGCTCAATGCTGAGTTGATTCAACTCAACAACACACTGGACGAGGAGAAAAAGCACGGTGATGAGTTGAGCCTTTTGTGCAAGGCGTTCAAGGCTCAATTTTGGTGGGCTTGTCCGATTAATGGGATGAATAGGGCTCAACTTGAATTATTCAAGAAGATTTTGGTGGAGCTTAAGAAACTTGTTGCTCATCATGTTGATAGGAGTGCAATTCAGGGTGCTTCTGCTCAAACTTTTCCATTTTCTGTTGGTAATGATTTATCCTCTAACATCCCTCTCCATTATCAGCCAAATCCTCAACAAAATGAGATATTTTCGCCACAAATTTTTCATAACCCTATGTTCAACCTCATTTAA

Protein sequence

>MS.gene92955.t1

MSSGRKGRGRQNIEMKKMTNERNLQVTFSKSRSGLFKKFSEFCTLCGVDVALVVFSPSEKVFSFGHPNVDTVIDRYLSGVPPQNKSTMEFIEAHRSAKVYELNAELIQLNNTLDEEKKHGDELSLLCKAFKAQFWWACPINGMNRAQLELFKKILVELKKLVAHHVDRSAIQGASAQTFPFSVGNDLSSNIPLHYQPNPQQNEIFSPQIFHNPMFNLI