Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93064.t1 | XP_013446213.1 | 95 | 120 | 6 | 0 | 1 | 120 | 1 | 120 | 5.60E-55 | 223.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93064.t1 | A0A072U339 | 95.0 | 120 | 6 | 0 | 1 | 120 | 1 | 120 | 4.1e-55 | 223.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056482 | MS.gene93064 | 0.954339 | 3.70E-112 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93064.t1 | MTR_8g070040 | 95.000 | 120 | 6 | 0 | 1 | 120 | 1 | 120 | 3.35e-78 | 226 |
| MS.gene93064.t1 | MTR_8g070015 | 93.805 | 113 | 7 | 0 | 1 | 113 | 1 | 113 | 1.56e-71 | 209 |
| MS.gene93064.t1 | MTR_7g065210 | 51.515 | 99 | 46 | 2 | 23 | 120 | 20 | 117 | 1.56e-26 | 95.5 |
| MS.gene93064.t1 | MTR_7g065160 | 51.579 | 95 | 44 | 2 | 27 | 120 | 24 | 117 | 6.92e-25 | 91.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93064.t1 | AT2G37870 | 67.797 | 118 | 35 | 1 | 3 | 120 | 1 | 115 | 1.64e-50 | 156 |
| MS.gene93064.t1 | AT5G05960 | 45.902 | 122 | 58 | 3 | 1 | 120 | 1 | 116 | 2.59e-29 | 102 |
| MS.gene93064.t1 | AT3G53980 | 46.809 | 94 | 49 | 1 | 27 | 120 | 22 | 114 | 2.03e-27 | 97.8 |
| MS.gene93064.t1 | AT3G53980 | 46.809 | 94 | 49 | 1 | 27 | 120 | 22 | 114 | 2.03e-27 | 97.8 |
Find 25 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCATCAAGTGTGTTAATTT+TGG | 0.223634 | 8.2:-32760648 | MS.gene93064:CDS |
| AGCTAGTCTAAGTCCATGTT+TGG | 0.350440 | 8.2:-32760576 | MS.gene93064:CDS |
| TTCCTAATGTTGCATCTCTT+AGG | 0.378053 | 8.2:+32760401 | None:intergenic |
| TGGGTGCTGTACGTAATGTT+AGG | 0.392658 | 8.2:-32760556 | MS.gene93064:CDS |
| GAATGTGGAAGGACACCAAT+TGG | 0.434211 | 8.2:-32760608 | MS.gene93064:CDS |
| AACAATAGTGATGGAGTTCA+AGG | 0.437224 | 8.2:-32760708 | None:intergenic |
| AGAACAGAACAAAGACATCT+TGG | 0.469439 | 8.2:+32760479 | None:intergenic |
| CTTCCACATTTCTTTCCAGC+AGG | 0.469545 | 8.2:+32760374 | None:intergenic |
| CCAACTCTAGCACAACAAAC+TGG | 0.478057 | 8.2:+32760521 | None:intergenic |
| CTAGCTGCTGCTGACCCAAT+TGG | 0.492373 | 8.2:+32760593 | None:intergenic |
| TTCCTAAGAGATGCAACATT+AGG | 0.493031 | 8.2:-32760403 | MS.gene93064:CDS |
| GGGTGCTGTACGTAATGTTA+GGG | 0.506383 | 8.2:-32760555 | MS.gene93064:CDS |
| CCAGTTTGTTGTGCTAGAGT+TGG | 0.508251 | 8.2:-32760521 | MS.gene93064:CDS |
| CACATTTCTTTCCAGCAGGT+CGG | 0.514268 | 8.2:+32760378 | None:intergenic |
| GCTGAGGCAGCTGGAGAATG+TGG | 0.520830 | 8.2:-32760623 | MS.gene93064:CDS |
| AATGTGGAAGGACACCAATT+GGG | 0.523864 | 8.2:-32760607 | MS.gene93064:CDS |
| AACATTAGGAACCGACCTGC+TGG | 0.527493 | 8.2:-32760389 | MS.gene93064:CDS |
| GCTAGTCTAAGTCCATGTTT+GGG | 0.544347 | 8.2:-32760575 | MS.gene93064:CDS |
| GGAACAGTGATAGCAATAGC+AGG | 0.582273 | 8.2:+32760422 | None:intergenic |
| TAGAACTTTCCTAGCCATAG+TGG | 0.583702 | 8.2:-32760684 | MS.gene93064:CDS |
| AAAACATCACCACTATGGCT+AGG | 0.592042 | 8.2:+32760675 | None:intergenic |
| TTACGTACAGCACCCAAACA+TGG | 0.602239 | 8.2:+32760563 | None:intergenic |
| AGGCAGCTGGAGAATGTGGA+AGG | 0.609221 | 8.2:-32760619 | MS.gene93064:CDS |
| CGACCTGCTGGAAAGAAATG+TGG | 0.609362 | 8.2:-32760377 | MS.gene93064:CDS |
| ACTCTAGCACAACAAACTGG+TGG | 0.633479 | 8.2:+32760524 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCATTTAAACTTTGTTTTT+AGG | + | chr8.2:32760480-32760499 | None:intergenic | 15.0% |
| !! | CAAAAATATGAATCAATGAA+AGG | + | chr8.2:32760407-32760426 | None:intergenic | 20.0% |
| !!! | CTTTCATTGATTCATATTTT+TGG | - | chr8.2:32760405-32760424 | MS.gene93064:CDS | 20.0% |
| ! | AGAGTTTGTAAAACTAAAAC+AGG | - | chr8.2:32760441-32760460 | MS.gene93064:CDS | 25.0% |
| ! | TATGAATCAATGAAAGGTTA+AGG | + | chr8.2:32760401-32760420 | None:intergenic | 25.0% |
| !!! | CTTCTTTTTTTTGCTTTACA+GGG | - | chr8.2:32760683-32760702 | MS.gene93064:CDS | 25.0% |
| !!! | TTAAGGTGTTTTCAAAACTA+AGG | + | chr8.2:32760384-32760403 | None:intergenic | 25.0% |
| AAATGTACTAAGTGTCTGTT+TGG | + | chr8.2:32760285-32760304 | None:intergenic | 30.0% | |
| CAAAAAAAACATCACCACTA+TGG | + | chr8.2:32759781-32759800 | None:intergenic | 30.0% | |
| CCTGTAAAGCAAAAAAAAGA+AGG | + | chr8.2:32760685-32760704 | None:intergenic | 30.0% | |
| TTCATATTGTACGCACAATA+AGG | + | chr8.2:32760196-32760215 | None:intergenic | 30.0% | |
| ! | GTTTGAAATAGTAAGACGTA+AGG | - | chr8.2:32760315-32760334 | MS.gene93064:intron | 30.0% |
| !!! | CATAGTGGTGATGTTTTTTT+TGG | - | chr8.2:32759779-32759798 | MS.gene93064:intron | 30.0% |
| !!! | CCTTCTTTTTTTTGCTTTAC+AGG | - | chr8.2:32760682-32760701 | MS.gene93064:CDS | 30.0% |
| AGAACAGAACAAAGACATCT+TGG | + | chr8.2:32759972-32759991 | None:intergenic | 35.0% | |
| TATGTTCTGTTTGTGTCAAG+TGG | + | chr8.2:32760544-32760563 | None:intergenic | 35.0% | |
| TTATCATTTCTCACATCCCA+AGG | + | chr8.2:32760249-32760268 | None:intergenic | 35.0% | |
| TTCCTAAGAGATGCAACATT+AGG | - | chr8.2:32760045-32760064 | MS.gene93064:intron | 35.0% | |
| TTCCTAATGTTGCATCTCTT+AGG | + | chr8.2:32760050-32760069 | None:intergenic | 35.0% | |
| ! | AGGTGTTTTCAAAACTAAGG+TGG | + | chr8.2:32760381-32760400 | None:intergenic | 35.0% |
| ! | GGCATCAAGTGTGTTAATTT+TGG | - | chr8.2:32759800-32759819 | MS.gene93064:intron | 35.0% |
| ! | TGTGCACATTTTGCAAAACT+TGG | - | chr8.2:32760564-32760583 | MS.gene93064:CDS | 35.0% |
| ! | TTCTGTTCTTTTGTCACCTA+TGG | - | chr8.2:32759983-32760002 | MS.gene93064:intron | 35.0% |
| !! | TGTGTTAATTTTGGAAGCTG+AGG | - | chr8.2:32759809-32759828 | MS.gene93064:intron | 35.0% |
| !!! | ATCTTAGCTTGTTTTGCCAT+AGG | + | chr8.2:32760002-32760021 | None:intergenic | 35.0% |
| AAAACATCACCACTATGGCT+AGG | + | chr8.2:32759776-32759795 | None:intergenic | 40.0% | |
| AAGGTCAAACTAAAGCCGAT+AGG | - | chr8.2:32760334-32760353 | MS.gene93064:intron | 40.0% | |
| AATGTGGAAGGACACCAATT+GGG | - | chr8.2:32759841-32759860 | MS.gene93064:intron | 40.0% | |
| TACGCACAATAAGGAATGTG+CGG | + | chr8.2:32760187-32760206 | None:intergenic | 40.0% | |
| TAGAACTTTCCTAGCCATAG+TGG | - | chr8.2:32759764-32759783 | MS.gene93064:intron | 40.0% | |
| ! | AGCTAGTCTAAGTCCATGTT+TGG | - | chr8.2:32759872-32759891 | MS.gene93064:intron | 40.0% |
| ! | GCTAGTCTAAGTCCATGTTT+GGG | - | chr8.2:32759873-32759892 | MS.gene93064:intron | 40.0% |
| AATGTCGACGCTACACCTAT+CGG | + | chr8.2:32760352-32760371 | None:intergenic | 45.0% | |
| ACTCTAGCACAACAAACTGG+TGG | + | chr8.2:32759927-32759946 | None:intergenic | 45.0% | |
| CACATTTCTTTCCAGCAGGT+CGG | + | chr8.2:32760073-32760092 | None:intergenic | 45.0% | |
| CCAACTCTAGCACAACAAAC+TGG | + | chr8.2:32759930-32759949 | None:intergenic | 45.0% | |
| CTTCCACATTTCTTTCCAGC+AGG | + | chr8.2:32760077-32760096 | None:intergenic | 45.0% | |
| GAATGTGGAAGGACACCAAT+TGG | - | chr8.2:32759840-32759859 | MS.gene93064:intron | 45.0% | |
| GGAACAGTGATAGCAATAGC+AGG | + | chr8.2:32760029-32760048 | None:intergenic | 45.0% | |
| TTACGTACAGCACCCAAACA+TGG | + | chr8.2:32759888-32759907 | None:intergenic | 45.0% | |
| ! | GGGTGCTGTACGTAATGTTA+GGG | - | chr8.2:32759893-32759912 | MS.gene93064:intron | 45.0% |
| ! | TGGGTGCTGTACGTAATGTT+AGG | - | chr8.2:32759892-32759911 | MS.gene93064:intron | 45.0% |
| !! | CCAGTTTGTTGTGCTAGAGT+TGG | - | chr8.2:32759927-32759946 | MS.gene93064:intron | 45.0% |
| AACATTAGGAACCGACCTGC+TGG | - | chr8.2:32760059-32760078 | MS.gene93064:intron | 50.0% | |
| ACTTGTGTCGCAAGTAGCCT+TGG | - | chr8.2:32760229-32760248 | MS.gene93064:intron | 50.0% | |
| CGACCTGCTGGAAAGAAATG+TGG | - | chr8.2:32760071-32760090 | MS.gene93064:intron | 50.0% | |
| CTTGTGTCGCAAGTAGCCTT+GGG | - | chr8.2:32760230-32760249 | MS.gene93064:intron | 50.0% | |
| !! | ATTTTGGAAGCTGAGGCAGC+TGG | - | chr8.2:32759816-32759835 | MS.gene93064:intron | 50.0% |
| AGGCAGCTGGAGAATGTGGA+AGG | - | chr8.2:32759829-32759848 | MS.gene93064:intron | 55.0% | |
| CTAGCTGCTGCTGACCCAAT+TGG | + | chr8.2:32759858-32759877 | None:intergenic | 55.0% | |
| GCTGAGGCAGCTGGAGAATG+TGG | - | chr8.2:32759825-32759844 | MS.gene93064:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 32759750 | 32760720 | 32759750 | ID=MS.gene93064 |
| chr8.2 | mRNA | 32759750 | 32760720 | 32759750 | ID=MS.gene93064.t1;Parent=MS.gene93064 |
| chr8.2 | exon | 32760375 | 32760720 | 32760375 | ID=MS.gene93064.t1.exon1;Parent=MS.gene93064.t1 |
| chr8.2 | CDS | 32760375 | 32760720 | 32760375 | ID=cds.MS.gene93064.t1;Parent=MS.gene93064.t1 |
| chr8.2 | exon | 32759750 | 32759766 | 32759750 | ID=MS.gene93064.t1.exon2;Parent=MS.gene93064.t1 |
| chr8.2 | CDS | 32759750 | 32759766 | 32759750 | ID=cds.MS.gene93064.t1;Parent=MS.gene93064.t1 |
| Gene Sequence |
| Protein sequence |
