Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056482.t1 | XP_013446213.1 | 95 | 120 | 6 | 0 | 1 | 120 | 1 | 120 | 5.60E-55 | 223.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056482.t1 | A0A072U339 | 95.0 | 120 | 6 | 0 | 1 | 120 | 1 | 120 | 4.1e-55 | 223.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056482 | MS.gene09712 | 0.815737 | 7.93E-52 | -1.69E-46 |
| MS.gene056482 | MS.gene21522 | 0.805971 | 1.03E-49 | -1.69E-46 |
| MS.gene056482 | MS.gene93064 | 0.954339 | 3.70E-112 | -1.69E-46 |
| MS.gene056482 | MS.gene97976 | 0.801767 | 7.71E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056482.t1 | MTR_8g070040 | 95.000 | 120 | 6 | 0 | 1 | 120 | 1 | 120 | 3.73e-78 | 226 |
| MS.gene056482.t1 | MTR_8g070015 | 93.805 | 113 | 7 | 0 | 1 | 113 | 1 | 113 | 2.06e-71 | 209 |
| MS.gene056482.t1 | MTR_7g065210 | 51.515 | 99 | 46 | 2 | 23 | 120 | 20 | 117 | 6.68e-27 | 96.7 |
| MS.gene056482.t1 | MTR_7g065160 | 51.579 | 95 | 44 | 2 | 27 | 120 | 24 | 117 | 2.93e-25 | 92.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056482.t1 | AT2G37870 | 67.797 | 118 | 35 | 1 | 3 | 120 | 1 | 115 | 1.83e-50 | 156 |
| MS.gene056482.t1 | AT5G05960 | 45.902 | 122 | 58 | 3 | 1 | 120 | 1 | 116 | 2.20e-29 | 102 |
| MS.gene056482.t1 | AT3G53980 | 46.809 | 94 | 49 | 1 | 27 | 120 | 22 | 114 | 1.84e-27 | 97.8 |
| MS.gene056482.t1 | AT3G53980 | 46.809 | 94 | 49 | 1 | 27 | 120 | 22 | 114 | 1.84e-27 | 97.8 |
Find 26 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCATCAAGTGTGTTAATTT+TGG | 0.223634 | 8.3:-32455751 | MS.gene056482:CDS |
| AGCTAGTCTAAGTCCATGTT+TGG | 0.350440 | 8.3:-32455679 | MS.gene056482:CDS |
| ATCTTAGCTTGTTTAGCAAT+AGG | 0.376748 | 8.3:+32455552 | None:intergenic |
| TTCCTAATGTTGCATCTCTT+AGG | 0.378053 | 8.3:+32455504 | None:intergenic |
| TGGGTGCTGTACGTAATGTT+AGG | 0.392658 | 8.3:-32455659 | MS.gene056482:CDS |
| GAATGTGGAAGGACACCAAT+TGG | 0.434211 | 8.3:-32455711 | MS.gene056482:CDS |
| AACAATAGTGATGGAGTTCA+AGG | 0.437224 | 8.3:-32455811 | None:intergenic |
| AGAACAGAACAAAGACATCT+TGG | 0.469439 | 8.3:+32455582 | None:intergenic |
| CTTCCACATTTCTTTCCAGC+AGG | 0.469545 | 8.3:+32455477 | None:intergenic |
| CCAACTCTAGCACAACAAAC+TGG | 0.478057 | 8.3:+32455624 | None:intergenic |
| CTAGCTGCTGCTGACCCAAT+TGG | 0.492373 | 8.3:+32455696 | None:intergenic |
| TTCCTAAGAGATGCAACATT+AGG | 0.493031 | 8.3:-32455506 | MS.gene056482:CDS |
| GGGTGCTGTACGTAATGTTA+GGG | 0.506383 | 8.3:-32455658 | MS.gene056482:CDS |
| CCAGTTTGTTGTGCTAGAGT+TGG | 0.508251 | 8.3:-32455624 | MS.gene056482:CDS |
| CACATTTCTTTCCAGCAGGT+CGG | 0.514268 | 8.3:+32455481 | None:intergenic |
| GCTGAGGCAGCTGGAGAATG+TGG | 0.520830 | 8.3:-32455726 | MS.gene056482:CDS |
| AATGTGGAAGGACACCAATT+GGG | 0.523864 | 8.3:-32455710 | MS.gene056482:CDS |
| AACATTAGGAACCGACCTGC+TGG | 0.527493 | 8.3:-32455492 | MS.gene056482:CDS |
| GCTAGTCTAAGTCCATGTTT+GGG | 0.544347 | 8.3:-32455678 | MS.gene056482:CDS |
| GGAACAGTGATAGCAATAGC+AGG | 0.582273 | 8.3:+32455525 | None:intergenic |
| TAGAACTTTCCTAGCCATAG+TGG | 0.583702 | 8.3:-32455787 | MS.gene056482:CDS |
| AAAACATCACCACTATGGCT+AGG | 0.592042 | 8.3:+32455778 | None:intergenic |
| TTACGTACAGCACCCAAACA+TGG | 0.602239 | 8.3:+32455666 | None:intergenic |
| AGGCAGCTGGAGAATGTGGA+AGG | 0.609221 | 8.3:-32455722 | MS.gene056482:CDS |
| CGACCTGCTGGAAAGAAATG+TGG | 0.609362 | 8.3:-32455480 | MS.gene056482:CDS |
| ACTCTAGCACAACAAACTGG+TGG | 0.633479 | 8.3:+32455627 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCATTTAAACTTTGTTTTT+AGG | + | chr8.3:32455583-32455602 | None:intergenic | 15.0% |
| !! | CAAAAATATGAATCAATGAA+AGG | + | chr8.3:32455510-32455529 | None:intergenic | 20.0% |
| !!! | CTTTCATTGATTCATATTTT+TGG | - | chr8.3:32455508-32455527 | MS.gene056482:CDS | 20.0% |
| ! | AGAGTTTGTAAAACTAAAAC+AGG | - | chr8.3:32455544-32455563 | MS.gene056482:CDS | 25.0% |
| ! | TATGAATCAATGAAAGGTTA+AGG | + | chr8.3:32455504-32455523 | None:intergenic | 25.0% |
| !!! | CTTCTTTTTTTTGCTTTACA+GGG | - | chr8.3:32455786-32455805 | MS.gene056482:CDS | 25.0% |
| !!! | TTAAGGTGTTTTCAAAACTA+AGG | + | chr8.3:32455487-32455506 | None:intergenic | 25.0% |
| AAATGTACTAAGTGTCTGTT+TGG | + | chr8.3:32455388-32455407 | None:intergenic | 30.0% | |
| CAAAAAAAACATCACCACTA+TGG | + | chr8.3:32454884-32454903 | None:intergenic | 30.0% | |
| CCTGTAAAGCAAAAAAAAGA+AGG | + | chr8.3:32455788-32455807 | None:intergenic | 30.0% | |
| TATGTTCTATTTGTGTCAAG+TGG | + | chr8.3:32455647-32455666 | None:intergenic | 30.0% | |
| TTCATATTGTACGCACAATA+AGG | + | chr8.3:32455299-32455318 | None:intergenic | 30.0% | |
| ! | GTTTGAAATAGTAAGACGTA+AGG | - | chr8.3:32455418-32455437 | MS.gene056482:intron | 30.0% |
| !! | ATCTTAGCTTGTTTAGCAAT+AGG | + | chr8.3:32455105-32455124 | None:intergenic | 30.0% |
| !!! | CATAGTGGTGATGTTTTTTT+TGG | - | chr8.3:32454882-32454901 | MS.gene056482:intron | 30.0% |
| !!! | CCTTCTTTTTTTTGCTTTAC+AGG | - | chr8.3:32455785-32455804 | MS.gene056482:CDS | 30.0% |
| AGAACAGAACAAAGACATCT+TGG | + | chr8.3:32455075-32455094 | None:intergenic | 35.0% | |
| TTATCATTTCTCACATCCCA+AGG | + | chr8.3:32455352-32455371 | None:intergenic | 35.0% | |
| TTCCTAAGAGATGCAACATT+AGG | - | chr8.3:32455148-32455167 | MS.gene056482:intron | 35.0% | |
| TTCCTAATGTTGCATCTCTT+AGG | + | chr8.3:32455153-32455172 | None:intergenic | 35.0% | |
| ! | AGGTGTTTTCAAAACTAAGG+TGG | + | chr8.3:32455484-32455503 | None:intergenic | 35.0% |
| ! | GGCATCAAGTGTGTTAATTT+TGG | - | chr8.3:32454903-32454922 | MS.gene056482:intron | 35.0% |
| ! | TGTGCACATTTTGCAAAACT+TGG | - | chr8.3:32455667-32455686 | MS.gene056482:CDS | 35.0% |
| !! | TGTGTTAATTTTGGAAGCTG+AGG | - | chr8.3:32454912-32454931 | MS.gene056482:intron | 35.0% |
| AAAACATCACCACTATGGCT+AGG | + | chr8.3:32454879-32454898 | None:intergenic | 40.0% | |
| AAGGTCAAACTAAAGCCGAT+AGG | - | chr8.3:32455437-32455456 | MS.gene056482:intron | 40.0% | |
| AATGTGGAAGGACACCAATT+GGG | - | chr8.3:32454944-32454963 | MS.gene056482:intron | 40.0% | |
| TACGCACAATAAGGAATGTG+CGG | + | chr8.3:32455290-32455309 | None:intergenic | 40.0% | |
| TAGAACTTTCCTAGCCATAG+TGG | - | chr8.3:32454867-32454886 | MS.gene056482:intron | 40.0% | |
| ! | AGCTAGTCTAAGTCCATGTT+TGG | - | chr8.3:32454975-32454994 | MS.gene056482:intron | 40.0% |
| ! | GCTAGTCTAAGTCCATGTTT+GGG | - | chr8.3:32454976-32454995 | MS.gene056482:intron | 40.0% |
| AATGTCGACGCTACACCTAT+CGG | + | chr8.3:32455455-32455474 | None:intergenic | 45.0% | |
| ACTCTAGCACAACAAACTGG+TGG | + | chr8.3:32455030-32455049 | None:intergenic | 45.0% | |
| CACATTTCTTTCCAGCAGGT+CGG | + | chr8.3:32455176-32455195 | None:intergenic | 45.0% | |
| CCAACTCTAGCACAACAAAC+TGG | + | chr8.3:32455033-32455052 | None:intergenic | 45.0% | |
| CTTCCACATTTCTTTCCAGC+AGG | + | chr8.3:32455180-32455199 | None:intergenic | 45.0% | |
| GAATGTGGAAGGACACCAAT+TGG | - | chr8.3:32454943-32454962 | MS.gene056482:intron | 45.0% | |
| GGAACAGTGATAGCAATAGC+AGG | + | chr8.3:32455132-32455151 | None:intergenic | 45.0% | |
| TTACGTACAGCACCCAAACA+TGG | + | chr8.3:32454991-32455010 | None:intergenic | 45.0% | |
| ! | GGGTGCTGTACGTAATGTTA+GGG | - | chr8.3:32454996-32455015 | MS.gene056482:intron | 45.0% |
| ! | TGGGTGCTGTACGTAATGTT+AGG | - | chr8.3:32454995-32455014 | MS.gene056482:intron | 45.0% |
| !! | CCAGTTTGTTGTGCTAGAGT+TGG | - | chr8.3:32455030-32455049 | MS.gene056482:intron | 45.0% |
| AACATTAGGAACCGACCTGC+TGG | - | chr8.3:32455162-32455181 | MS.gene056482:intron | 50.0% | |
| ACTTGTGTCGCAAGTAGCCT+TGG | - | chr8.3:32455332-32455351 | MS.gene056482:intron | 50.0% | |
| CGACCTGCTGGAAAGAAATG+TGG | - | chr8.3:32455174-32455193 | MS.gene056482:intron | 50.0% | |
| CTTGTGTCGCAAGTAGCCTT+GGG | - | chr8.3:32455333-32455352 | MS.gene056482:intron | 50.0% | |
| !! | ATTTTGGAAGCTGAGGCAGC+TGG | - | chr8.3:32454919-32454938 | MS.gene056482:intron | 50.0% |
| AGGCAGCTGGAGAATGTGGA+AGG | - | chr8.3:32454932-32454951 | MS.gene056482:intron | 55.0% | |
| CTAGCTGCTGCTGACCCAAT+TGG | + | chr8.3:32454961-32454980 | None:intergenic | 55.0% | |
| GCTGAGGCAGCTGGAGAATG+TGG | - | chr8.3:32454928-32454947 | MS.gene056482:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 32454853 | 32455823 | 32454853 | ID=MS.gene056482 |
| chr8.3 | mRNA | 32454853 | 32455823 | 32454853 | ID=MS.gene056482.t1;Parent=MS.gene056482 |
| chr8.3 | exon | 32455478 | 32455823 | 32455478 | ID=MS.gene056482.t1.exon1;Parent=MS.gene056482.t1 |
| chr8.3 | CDS | 32455478 | 32455823 | 32455478 | ID=cds.MS.gene056482.t1;Parent=MS.gene056482.t1 |
| chr8.3 | exon | 32454853 | 32454869 | 32454853 | ID=MS.gene056482.t1.exon2;Parent=MS.gene056482.t1 |
| chr8.3 | CDS | 32454853 | 32454869 | 32454853 | ID=cds.MS.gene056482.t1;Parent=MS.gene056482.t1 |
| Gene Sequence |
| Protein sequence |
