Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93086.t1 | PNX96101.1 | 58.6 | 140 | 42 | 2 | 1 | 124 | 1 | 140 | 2.60E-36 | 161.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93086.t1 | A0A2K3MZ73 | 58.6 | 140 | 42 | 2 | 1 | 124 | 1 | 140 | 1.9e-36 | 161.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052447 | MS.gene93086 | 0.805288 | 1.43E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTCGTCTTGTTTGATTTA+AGG | 0.037211 | 8.2:-33043295 | None:intergenic |
| TGGGGCAGTGTTTGGGGTTT+GGG | 0.153802 | 8.2:+33043643 | MS.gene93086:CDS |
| GTGTTTGGGGTTTGGGAGTT+GGG | 0.212269 | 8.2:+33043650 | MS.gene93086:CDS |
| ATGGGGCAGTGTTTGGGGTT+TGG | 0.214708 | 8.2:+33043642 | MS.gene93086:CDS |
| AAGTAGTGATCGACAAATTT+TGG | 0.215233 | 8.2:-33043365 | None:intergenic |
| CCTGAGCATGGGGCAGTGTT+TGG | 0.221032 | 8.2:+33043635 | MS.gene93086:CDS |
| CGGACATGACATTGATTTAA+AGG | 0.222984 | 8.2:+33043693 | MS.gene93086:CDS |
| CTGAGCATGGGGCAGTGTTT+GGG | 0.226392 | 8.2:+33043636 | MS.gene93086:CDS |
| ACACCATTCGTTGACGTCTA+TGG | 0.231944 | 8.2:-33043128 | None:intergenic |
| AGTGTTTGGGGTTTGGGAGT+TGG | 0.279528 | 8.2:+33043649 | MS.gene93086:CDS |
| AAATGAGAGCAAAACAAGTT+TGG | 0.299455 | 8.2:+33042984 | None:intergenic |
| GAGTTGGGATTTCCCCTTCT+AGG | 0.372583 | 8.2:+33043665 | MS.gene93086:CDS |
| TTATGACGCTGGCGAGTATT+GGG | 0.388766 | 8.2:+33043214 | MS.gene93086:CDS |
| AATGAGAGCAAAACAAGTTT+GGG | 0.397378 | 8.2:+33042985 | None:intergenic |
| TCATGTCCGAACTTCCTAGA+AGG | 0.409490 | 8.2:-33043679 | None:intergenic |
| TTGTTGGTGACTAGATTTCT+TGG | 0.411961 | 8.2:+33043076 | MS.gene93086:CDS |
| ATTATGACGCTGGCGAGTAT+TGG | 0.416058 | 8.2:+33043213 | MS.gene93086:CDS |
| AACTCCTGATGATGTGGATA+AGG | 0.427990 | 8.2:+33043334 | MS.gene93086:CDS |
| ATTTCCCCTTCTAGGAAGTT+CGG | 0.434336 | 8.2:+33043673 | MS.gene93086:CDS |
| GTCACCAACAACGATTCATT+TGG | 0.452802 | 8.2:-33043064 | None:intergenic |
| GATTTCTTGGCGAGGTTGCT+AGG | 0.453315 | 8.2:+33043089 | MS.gene93086:CDS |
| TGAGCATGGGGCAGTGTTTG+GGG | 0.467867 | 8.2:+33043637 | MS.gene93086:CDS |
| TAGCGCTAAGTTGGAAGCGC+TGG | 0.475821 | 8.2:+33043249 | MS.gene93086:CDS |
| CACCATTCGTTGACGTCTAT+GGG | 0.486883 | 8.2:-33043127 | None:intergenic |
| CCAAACACTGCCCCATGCTC+AGG | 0.489905 | 8.2:-33043635 | None:intergenic |
| TGGGACCTGTCACCTGAGCA+TGG | 0.498264 | 8.2:+33043623 | MS.gene93086:CDS |
| TGAAGAGCTTAGCGCTAAGT+TGG | 0.499911 | 8.2:+33043240 | MS.gene93086:CDS |
| TGAACCTTATCCACATCATC+AGG | 0.518931 | 8.2:-33043338 | None:intergenic |
| ATTGATAACGACAGAGTAGC+AGG | 0.523233 | 8.2:+33043040 | MS.gene93086:CDS |
| CTGCCCCATGCTCAGGTGAC+AGG | 0.526102 | 8.2:-33043628 | None:intergenic |
| GGGACCTGTCACCTGAGCAT+GGG | 0.527720 | 8.2:+33043624 | MS.gene93086:CDS |
| AGGACCAAATGAATCGTTGT+TGG | 0.535995 | 8.2:+33043060 | MS.gene93086:CDS |
| CTACTTAAGTGATAGAATGA+AGG | 0.557840 | 8.2:+33043382 | MS.gene93086:CDS |
| TTCAGTTTAGATTATGACGC+TGG | 0.569234 | 8.2:+33043203 | MS.gene93086:intron |
| CATGTCCGAACTTCCTAGAA+GGG | 0.570347 | 8.2:-33043678 | None:intergenic |
| TTCCCATAGACGTCAACGAA+TGG | 0.604999 | 8.2:+33043125 | MS.gene93086:CDS |
| GGACCTGTCACCTGAGCATG+GGG | 0.618191 | 8.2:+33043625 | MS.gene93086:CDS |
| ACGAATGGTGTCTAATGCCA+AGG | 0.618696 | 8.2:+33043140 | MS.gene93086:CDS |
| ATGTCCGAACTTCCTAGAAG+GGG | 0.625510 | 8.2:-33043677 | None:intergenic |
| TAAATCAAACAAGACGAAGG+AGG | 0.675968 | 8.2:+33043298 | MS.gene93086:CDS |
| TTCTTGGCGAGGTTGCTAGG+AGG | 0.680138 | 8.2:+33043092 | MS.gene93086:CDS |
| CCTTAAATCAAACAAGACGA+AGG | 0.684217 | 8.2:+33043295 | MS.gene93086:CDS |
| GGTGACTAGATTTCTTGGCG+AGG | 0.689989 | 8.2:+33043081 | MS.gene93086:CDS |
| ATGATGTGGATAAGGTTCAG+TGG | 0.722685 | 8.2:+33043342 | MS.gene93086:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTTTTAAAGAAAATATAAAT+AGG | - | chr8.2:33043582-33043601 | None:intergenic | 10.0% |
| !!! | TCTTTAAAACTTTGTTTAAT+TGG | + | chr8.2:33043592-33043611 | MS.gene93086:intron | 15.0% |
| !! | AGATAAAAAATTCAAGGTTA+AGG | + | chr8.2:33043012-33043031 | MS.gene93086:CDS | 20.0% |
| !! | GGAAATAGATAAAAAATTCA+AGG | + | chr8.2:33043006-33043025 | MS.gene93086:CDS | 20.0% |
| !!! | AGATTCAATGATATTTTTGA+AGG | + | chr8.2:33043175-33043194 | MS.gene93086:intron | 20.0% |
| !!! | TGTTTAATTGGAAAAGTATT+GGG | + | chr8.2:33043604-33043623 | MS.gene93086:intron | 20.0% |
| !!! | TTGTTTAATTGGAAAAGTAT+TGG | + | chr8.2:33043603-33043622 | MS.gene93086:intron | 20.0% |
| ! | TTACTTCCTATATTTCTCAA+AGG | - | chr8.2:33043483-33043502 | None:intergenic | 25.0% |
| !! | AATCTATCTTTTATTGACCT+TGG | - | chr8.2:33043160-33043179 | None:intergenic | 25.0% |
| !! | TTGTTTCCTTTGAGAAATAT+AGG | + | chr8.2:33043474-33043493 | MS.gene93086:intron | 25.0% |
| CTACTTAAGTGATAGAATGA+AGG | + | chr8.2:33043382-33043401 | MS.gene93086:CDS | 30.0% | |
| TAAAATCCACTACTAGAAGA+TGG | + | chr8.2:33043534-33043553 | MS.gene93086:intron | 30.0% | |
| ! | AAGTAGTGATCGACAAATTT+TGG | - | chr8.2:33043368-33043387 | None:intergenic | 30.0% |
| ! | TTGTTCTACGTATTCTAGAA+AGG | + | chr8.2:33043432-33043451 | MS.gene93086:intron | 30.0% |
| !! | TAAGGTATTTTTCTCGCAAA+TGG | - | chr8.2:33043280-33043299 | None:intergenic | 30.0% |
| CCTTAAATCAAACAAGACGA+AGG | + | chr8.2:33043295-33043314 | MS.gene93086:CDS | 35.0% | |
| TAAATCAAACAAGACGAAGG+AGG | + | chr8.2:33043298-33043317 | MS.gene93086:CDS | 35.0% | |
| TTCAGTTTAGATTATGACGC+TGG | + | chr8.2:33043203-33043222 | MS.gene93086:intron | 35.0% | |
| ! | AAGATGGTTTGGAGAAACAA+AGG | + | chr8.2:33043550-33043569 | MS.gene93086:intron | 35.0% |
| ! | TGAAAAAACTCCTGATGATG+TGG | + | chr8.2:33043328-33043347 | MS.gene93086:CDS | 35.0% |
| !! | CCTTCGTCTTGTTTGATTTA+AGG | - | chr8.2:33043298-33043317 | None:intergenic | 35.0% |
| !! | TTGTTGGTGACTAGATTTCT+TGG | + | chr8.2:33043076-33043095 | MS.gene93086:CDS | 35.0% |
| AGGACCAAATGAATCGTTGT+TGG | + | chr8.2:33043060-33043079 | MS.gene93086:CDS | 40.0% | |
| ATGATGTGGATAAGGTTCAG+TGG | + | chr8.2:33043342-33043361 | MS.gene93086:CDS | 40.0% | |
| GTCACCAACAACGATTCATT+TGG | - | chr8.2:33043067-33043086 | None:intergenic | 40.0% | |
| TCCAAACCATCTTCTAGTAG+TGG | - | chr8.2:33043543-33043562 | None:intergenic | 40.0% | |
| TCCACTACTAGAAGATGGTT+TGG | + | chr8.2:33043539-33043558 | MS.gene93086:intron | 40.0% | |
| TGAACCTTATCCACATCATC+AGG | - | chr8.2:33043341-33043360 | None:intergenic | 40.0% | |
| ! | AACTCCTGATGATGTGGATA+AGG | + | chr8.2:33043334-33043353 | MS.gene93086:CDS | 40.0% |
| ! | ATTTCCCCTTCTAGGAAGTT+CGG | + | chr8.2:33043673-33043692 | MS.gene93086:CDS | 40.0% |
| !! | ATTGATAACGACAGAGTAGC+AGG | + | chr8.2:33043040-33043059 | MS.gene93086:CDS | 40.0% |
| ACACCATTCGTTGACGTCTA+TGG | - | chr8.2:33043131-33043150 | None:intergenic | 45.0% | |
| ACGAATGGTGTCTAATGCCA+AGG | + | chr8.2:33043140-33043159 | MS.gene93086:CDS | 45.0% | |
| ATGTCCGAACTTCCTAGAAG+GGG | - | chr8.2:33043680-33043699 | None:intergenic | 45.0% | |
| ATTATGACGCTGGCGAGTAT+TGG | + | chr8.2:33043213-33043232 | MS.gene93086:CDS | 45.0% | |
| CACCATTCGTTGACGTCTAT+GGG | - | chr8.2:33043130-33043149 | None:intergenic | 45.0% | |
| CATGTCCGAACTTCCTAGAA+GGG | - | chr8.2:33043681-33043700 | None:intergenic | 45.0% | |
| TCATGTCCGAACTTCCTAGA+AGG | - | chr8.2:33043682-33043701 | None:intergenic | 45.0% | |
| TGAAGAGCTTAGCGCTAAGT+TGG | + | chr8.2:33043240-33043259 | MS.gene93086:CDS | 45.0% | |
| TTATGACGCTGGCGAGTATT+GGG | + | chr8.2:33043214-33043233 | MS.gene93086:CDS | 45.0% | |
| TTCCCATAGACGTCAACGAA+TGG | + | chr8.2:33043125-33043144 | MS.gene93086:CDS | 45.0% | |
| GAGTTGGGATTTCCCCTTCT+AGG | + | chr8.2:33043665-33043684 | MS.gene93086:CDS | 50.0% | |
| GATTTCTTGGCGAGGTTGCT+AGG | + | chr8.2:33043089-33043108 | MS.gene93086:CDS | 50.0% | |
| GGTGACTAGATTTCTTGGCG+AGG | + | chr8.2:33043081-33043100 | MS.gene93086:CDS | 50.0% | |
| ! | AGTGTTTGGGGTTTGGGAGT+TGG | + | chr8.2:33043649-33043668 | MS.gene93086:CDS | 50.0% |
| ! | GTGTTTGGGGTTTGGGAGTT+GGG | + | chr8.2:33043650-33043669 | MS.gene93086:CDS | 50.0% |
| CTGAGCATGGGGCAGTGTTT+GGG | + | chr8.2:33043636-33043655 | MS.gene93086:CDS | 55.0% | |
| TGAGCATGGGGCAGTGTTTG+GGG | + | chr8.2:33043637-33043656 | MS.gene93086:CDS | 55.0% | |
| TTCTTGGCGAGGTTGCTAGG+AGG | + | chr8.2:33043092-33043111 | MS.gene93086:CDS | 55.0% | |
| ! | ATGGGGCAGTGTTTGGGGTT+TGG | + | chr8.2:33043642-33043661 | MS.gene93086:CDS | 55.0% |
| ! | TAGCGCTAAGTTGGAAGCGC+TGG | + | chr8.2:33043249-33043268 | MS.gene93086:CDS | 55.0% |
| ! | TGGGGCAGTGTTTGGGGTTT+GGG | + | chr8.2:33043643-33043662 | MS.gene93086:CDS | 55.0% |
| CCAAACACTGCCCCATGCTC+AGG | - | chr8.2:33043638-33043657 | None:intergenic | 60.0% | |
| CCTGAGCATGGGGCAGTGTT+TGG | + | chr8.2:33043635-33043654 | MS.gene93086:CDS | 60.0% | |
| GGACCTGTCACCTGAGCATG+GGG | + | chr8.2:33043625-33043644 | MS.gene93086:CDS | 60.0% | |
| GGGACCTGTCACCTGAGCAT+GGG | + | chr8.2:33043624-33043643 | MS.gene93086:CDS | 60.0% | |
| TGGGACCTGTCACCTGAGCA+TGG | + | chr8.2:33043623-33043642 | MS.gene93086:CDS | 60.0% | |
| CTGCCCCATGCTCAGGTGAC+AGG | - | chr8.2:33043631-33043650 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 33042986 | 33043712 | 33042986 | ID=MS.gene93086 |
| chr8.2 | mRNA | 33042986 | 33043712 | 33042986 | ID=MS.gene93086.t1;Parent=MS.gene93086 |
| chr8.2 | exon | 33042986 | 33043161 | 33042986 | ID=MS.gene93086.t1.exon1;Parent=MS.gene93086.t1 |
| chr8.2 | CDS | 33042986 | 33043161 | 33042986 | ID=cds.MS.gene93086.t1;Parent=MS.gene93086.t1 |
| chr8.2 | exon | 33043208 | 33043403 | 33043208 | ID=MS.gene93086.t1.exon2;Parent=MS.gene93086.t1 |
| chr8.2 | CDS | 33043208 | 33043403 | 33043208 | ID=cds.MS.gene93086.t1;Parent=MS.gene93086.t1 |
| chr8.2 | exon | 33043620 | 33043712 | 33043620 | ID=MS.gene93086.t1.exon3;Parent=MS.gene93086.t1 |
| chr8.2 | CDS | 33043620 | 33043712 | 33043620 | ID=cds.MS.gene93086.t1;Parent=MS.gene93086.t1 |
| Gene Sequence |
| Protein sequence |
