Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93203.t1 | GAU19423.1 | 69.7 | 66 | 20 | 0 | 1 | 66 | 227 | 292 | 1.60E-18 | 101.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93203.t1 | Q9LDD4 | 69.0 | 42 | 12 | 1 | 1 | 42 | 510 | 550 | 2.2e-08 | 59.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93203.t1 | A0A2Z6MBA3 | 69.7 | 66 | 20 | 0 | 1 | 66 | 227 | 292 | 1.1e-18 | 101.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053008 | MS.gene93203 | 0.807068 | 6.05E-50 | -1.69E-46 |
| MS.gene05979 | MS.gene93203 | 0.802137 | 6.47E-49 | -1.69E-46 |
| MS.gene060803 | MS.gene93203 | 0.826943 | 2.06E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93203.t1 | MTR_8g098645 | 100.000 | 42 | 0 | 0 | 1 | 42 | 281 | 322 | 1.98e-24 | 93.6 |
| MS.gene93203.t1 | MTR_1g073420 | 90.909 | 44 | 4 | 0 | 1 | 44 | 69 | 112 | 2.86e-24 | 88.6 |
| MS.gene93203.t1 | MTR_1g073590 | 88.636 | 44 | 5 | 0 | 1 | 44 | 520 | 563 | 6.46e-21 | 85.1 |
| MS.gene93203.t1 | MTR_8g098605 | 86.364 | 44 | 6 | 0 | 1 | 44 | 531 | 574 | 7.87e-21 | 84.7 |
| MS.gene93203.t1 | MTR_1g073530 | 88.636 | 44 | 5 | 0 | 1 | 44 | 523 | 566 | 1.41e-20 | 84.0 |
| MS.gene93203.t1 | MTR_0259s0060 | 85.366 | 41 | 6 | 0 | 1 | 41 | 107 | 147 | 1.80e-19 | 77.4 |
| MS.gene93203.t1 | MTR_1g073380 | 84.091 | 44 | 7 | 0 | 1 | 44 | 533 | 576 | 4.11e-19 | 79.7 |
| MS.gene93203.t1 | MTR_8g098635 | 79.545 | 44 | 9 | 0 | 1 | 44 | 232 | 275 | 3.06e-18 | 76.3 |
| MS.gene93203.t1 | MTR_8g098635 | 79.545 | 44 | 9 | 0 | 1 | 44 | 315 | 358 | 1.19e-17 | 75.5 |
| MS.gene93203.t1 | MTR_7g078120 | 64.286 | 42 | 11 | 1 | 1 | 38 | 525 | 566 | 4.35e-12 | 59.7 |
| MS.gene93203.t1 | MTR_7g078120 | 64.286 | 42 | 11 | 1 | 1 | 38 | 525 | 566 | 4.73e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93203.t1 | AT4G11400 | 69.048 | 42 | 12 | 1 | 1 | 42 | 510 | 550 | 8.36e-13 | 62.0 |
| MS.gene93203.t1 | AT4G11400 | 69.048 | 42 | 12 | 1 | 1 | 42 | 510 | 550 | 8.36e-13 | 62.0 |
Find 6 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGATGACGAAGTTGAATT+TGG | 0.250331 | 4.2:-6250886 | MS.gene93203:CDS |
| CACTCCAAGCAATCCATATA+TGG | 0.312317 | 4.2:+6250824 | None:intergenic |
| AGCTATCAAGCATCCATATA+TGG | 0.363115 | 4.2:-6250837 | MS.gene93203:CDS |
| GAAGTTGAATTTGGATCTTT+TGG | 0.375334 | 4.2:-6250877 | MS.gene93203:CDS |
| GCATCCATATATGGATTGCT+TGG | 0.469079 | 4.2:-6250828 | MS.gene93203:CDS |
| TCATCGCTGTCAACAGTCTT+CGG | 0.477687 | 4.2:+6250904 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAAGTTGAATTTGGATCTTT+TGG | - | chr4.2:6250846-6250865 | MS.gene93203:CDS | 30.0% |
| AGCTATCAAGCATCCATATA+TGG | - | chr4.2:6250886-6250905 | MS.gene93203:CDS | 35.0% | |
| ! | GAATTTGGATCTTTTGGTGA+TGG | - | chr4.2:6250852-6250871 | MS.gene93203:CDS | 35.0% |
| ! | GATGATGACGAAGTTGAATT+TGG | - | chr4.2:6250837-6250856 | MS.gene93203:CDS | 35.0% |
| CACTCCAAGCAATCCATATA+TGG | + | chr4.2:6250902-6250921 | None:intergenic | 40.0% | |
| ! | GCATCCATATATGGATTGCT+TGG | - | chr4.2:6250895-6250914 | MS.gene93203:CDS | 40.0% |
| !! | AAGTGCGATAAGCCATTTTG+AGG | - | chr4.2:6250962-6250981 | MS.gene93203:CDS | 40.0% |
| !!! | CTTTTGGTGATGGTTTTGGA+GGG | - | chr4.2:6250862-6250881 | MS.gene93203:CDS | 40.0% |
| !!! | GGATCTTTTGGTGATGGTTT+TGG | - | chr4.2:6250858-6250877 | MS.gene93203:CDS | 40.0% |
| !!! | TCTTTTGGTGATGGTTTTGG+AGG | - | chr4.2:6250861-6250880 | MS.gene93203:CDS | 40.0% |
| TCATCGCTGTCAACAGTCTT+CGG | + | chr4.2:6250822-6250841 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 6250756 | 6250989 | 6250756 | ID=MS.gene93203 |
| chr4.2 | mRNA | 6250756 | 6250989 | 6250756 | ID=MS.gene93203.t1;Parent=MS.gene93203 |
| chr4.2 | exon | 6250756 | 6250989 | 6250756 | ID=MS.gene93203.t1.exon1;Parent=MS.gene93203.t1 |
| chr4.2 | CDS | 6250756 | 6250989 | 6250756 | ID=cds.MS.gene93203.t1;Parent=MS.gene93203.t1 |
| Gene Sequence |
| Protein sequence |
