AlfalfaGEDB | MS.gene93658

M. sativa cultivar XinJiangDaYe / MS.gene93658

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93658.t1	KEH23134.1	90.3	226	22	0	1	226	166	391	8.00E-119	436.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93658.t1	A0A072U022	90.3	226	22	0	1	226	166	391	5.8e-119	436.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052447	MS.gene93658	0.820587	6.34E-53	-1.69E-46
MS.gene059460	MS.gene93658	0.81853	1.87E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93658.t1	MTR_7g066890	90.265	226	22	0	1	226	64	289	5.32e-145	409
MS.gene93658.t1	MTR_7g066890	90.265	226	22	0	1	226	166	391	1.28e-143	409
MS.gene93658.t1	MTR_7g066880	55.111	225	94	1	2	226	64	281	7.40e-79	241
MS.gene93658.t1	MTR_7g066920	75.000	92	23	0	87	178	1	92	9.03e-44	142
MS.gene93658.t1	MTR_7g067580	43.646	181	73	2	24	176	30	209	9.21e-40	136
MS.gene93658.t1	MTR_8g098880	41.477	176	76	1	28	176	111	286	2.42e-38	134
MS.gene93658.t1	MTR_7g066970	42.073	164	68	1	40	176	113	276	2.77e-24	97.8
MS.gene93658.t1	MTR_7g078120	41.000	100	51	2	127	224	383	476	4.62e-15	73.9
MS.gene93658.t1	MTR_7g078120	41.000	100	51	2	127	224	383	476	4.72e-15	73.9
MS.gene93658.t1	MTR_1g073380	40.404	99	51	2	130	226	394	486	8.96e-14	70.1
MS.gene93658.t1	MTR_8g098645	38.384	99	53	2	130	226	142	234	1.94e-12	65.9
MS.gene93658.t1	MTR_1g073530	39.796	98	51	2	131	226	385	476	2.55e-12	65.9
MS.gene93658.t1	MTR_1g073590	39.796	98	51	2	131	226	382	473	2.67e-12	65.9
MS.gene93658.t1	MTR_8g098635	41.758	91	45	2	138	226	101	185	4.31e-12	64.7
MS.gene93658.t1	MTR_8g098635	41.758	91	45	2	138	226	184	268	4.72e-12	64.7
MS.gene93658.t1	MTR_8g098605	37.374	99	54	2	130	226	392	484	5.94e-12	64.7
MS.gene93658.t1	MTR_8g028905	34.783	115	63	3	116	224	390	498	8.40e-12	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93658.t1	AT5G04110	41.844	141	73	3	95	226	322	462	2.36e-26	106
MS.gene93658.t1	AT5G04110	40.816	147	78	3	89	226	315	461	2.38e-26	106
MS.gene93658.t1	AT5G04110	41.844	141	73	3	95	226	321	461	2.81e-26	106
MS.gene93658.t1	AT5G04110	41.844	141	73	3	95	226	321	461	2.81e-26	106
MS.gene93658.t1	AT2G46040	40.187	107	47	3	128	226	371	468	9.56e-14	70.1
MS.gene93658.t1	AT2G46040	40.187	107	47	3	128	226	357	454	1.02e-13	70.1
MS.gene93658.t1	AT2G46040	40.187	107	47	3	128	226	357	454	1.02e-13	70.1
MS.gene93658.t1	AT4G11400	32.086	187	104	7	55	226	289	467	6.99e-11	61.6
MS.gene93658.t1	AT4G11400	32.086	187	104	7	55	226	289	467	6.99e-11	61.6

Find 47 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAAGGCATCGGCCAAATT+TGG	0.174872	7.1:-34725884	None:intergenic
AGATTTATTTATGTTGGTTT+TGG	0.214649	7.1:-34725829	None:intergenic
TATCCACATTAAAGATTTAA+TGG	0.219230	7.1:+34725632	MS.gene93658:CDS
TGACCGCAATCAACTGATTC+AGG	0.246249	7.1:-34725969	None:intergenic
AGTGGTTGGGCACCCAAATT+TGG	0.252889	7.1:+34725871	MS.gene93658:CDS
TTGGGTGCCTTCTTCGTTCT+TGG	0.260964	7.1:-34725416	None:intergenic
TTCTTCTTGATTGATTTCTT+AGG	0.293081	7.1:-34725678	None:intergenic
TCCAGGTTCACTTGATTCAT+AGG	0.374616	7.1:-34725609	None:intergenic
AGAAGGCATCGGCCAAATTT+GGG	0.394978	7.1:-34725883	None:intergenic
CTAGGTCCGATGGGAATAAC+TGG	0.404012	7.1:-34725777	None:intergenic
AATCTTTAATGTGGATATCC+AGG	0.417219	7.1:-34725626	None:intergenic
CGAAGAAGGCACCCAACTCT+TGG	0.437330	7.1:+34725423	MS.gene93658:CDS
CCGATGGGAATAACTGGTCT+TGG	0.438746	7.1:-34725771	None:intergenic
TTCAAGCGGAAGTTCCCATA+TGG	0.439747	7.1:+34725802	MS.gene93658:CDS
TTATCTAAAACCAATGCAAA+AGG	0.454766	7.1:+34725906	MS.gene93658:CDS
GTTATTAGATTTATTTATGT+TGG	0.467322	7.1:-34725835	None:intergenic
CAATGATTCTATCAAGGGAT+CGG	0.468598	7.1:+34725581	MS.gene93658:CDS
AATGATTCTATCAAGGGATC+GGG	0.471189	7.1:+34725582	MS.gene93658:CDS
TGACTTCAATGATTCTATCA+AGG	0.477723	7.1:+34725575	MS.gene93658:CDS
CGATGATTGTTTGAAGTGGT+TGG	0.480840	7.1:+34725857	MS.gene93658:CDS
GACATGAGAGGCCAAGAGTT+GGG	0.489477	7.1:-34725434	None:intergenic
CGCTTGAAACCTAGGTCCGA+TGG	0.493771	7.1:-34725787	None:intergenic
GATGATTGTTTGAAGTGGTT+GGG	0.496066	7.1:+34725858	MS.gene93658:CDS
CCAACTTGAAAATGCGGTAC+CGG	0.502800	7.1:-34726033	None:intergenic
TAGAATCATTGAAGTCACTT+AGG	0.508342	7.1:-34725569	None:intergenic
GGAACTTCCGCTTGAAACCT+AGG	0.508500	7.1:-34725795	None:intergenic
TCAAGCGGAAGTTCCCATAT+GGG	0.520349	7.1:+34725803	MS.gene93658:CDS
CAGTTATTCCCATCGGACCT+AGG	0.530953	7.1:+34725778	MS.gene93658:CDS
TCCTATGAATCAAGTGAACC+TGG	0.531531	7.1:+34725608	MS.gene93658:CDS
GAATAACTGGTCTTGGAAGA+TGG	0.535998	7.1:-34725764	None:intergenic
AAAATGTCCAAGAACGAAGA+AGG	0.539829	7.1:+34725409	MS.gene93658:CDS
CATCCATTAAATCTTTAATG+TGG	0.548632	7.1:-34725635	None:intergenic
GACTTCAATGATTCTATCAA+GGG	0.551326	7.1:+34725576	MS.gene93658:CDS
GAATGCTTAAAATTCAAGTC+CGG	0.559221	7.1:+34726014	MS.gene93658:CDS
AATGCAAAAGGTATCGATGA+AGG	0.561454	7.1:+34725918	MS.gene93658:CDS
TCATGTCCATCACCACGTGA+TGG	0.590594	7.1:+34725450	MS.gene93658:CDS
CTCATTGATACAATGAAACA+TGG	0.590636	7.1:+34725381	None:intergenic
ATAACGATGATTGTTTGAAG+TGG	0.592396	7.1:+34725853	MS.gene93658:CDS
CAAAAGGTATCGATGAAGGT+AGG	0.595054	7.1:+34725922	MS.gene93658:CDS
CCAAGACCAGTTATTCCCAT+CGG	0.603424	7.1:+34725771	MS.gene93658:CDS
CATCGGACCTAGGTTTCAAG+CGG	0.620694	7.1:+34725788	MS.gene93658:CDS
GGACATGAGAGGCCAAGAGT+TGG	0.623154	7.1:-34725435	None:intergenic
GCTTGAAACCTAGGTCCGAT+GGG	0.632753	7.1:-34725786	None:intergenic
AAACTTCCAACTTGAAAATG+CGG	0.649252	7.1:-34726039	None:intergenic
AGCGGAAGTTCCCATATGGG+AGG	0.653379	7.1:+34725806	MS.gene93658:CDS
AATCCTGAATCAGTTGATTG+CGG	0.666835	7.1:+34725966	MS.gene93658:CDS
CACGTGGTGATGGACATGAG+AGG	0.700862	7.1:-34725446	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTTATTAGATTTATTTATGT+TGG	-	chr7.1:34725838-34725857	None:intergenic	15.0%
!!	TATCCACATTAAAGATTTAA+TGG	+	chr7.1:34725632-34725651	MS.gene93658:CDS	20.0%
!!!	AGATTTATTTATGTTGGTTT+TGG	-	chr7.1:34725832-34725851	None:intergenic	20.0%
!	CATCCATTAAATCTTTAATG+TGG	-	chr7.1:34725638-34725657	None:intergenic	25.0%
!	GGAAAAAGAATTATTGTACA+AGG	+	chr7.1:34725471-34725490	MS.gene93658:CDS	25.0%
!!	AATGGTAAAGAAGAATTTTG+TGG	+	chr7.1:34725522-34725541	MS.gene93658:CDS	25.0%
!!	AATTCTTCTTTACCATTTTG+TGG	-	chr7.1:34725519-34725538	None:intergenic	25.0%
!!	ATTCTTCTTTACCATTTTGT+GGG	-	chr7.1:34725518-34725537	None:intergenic	25.0%
!!	TTATCTAAAACCAATGCAAA+AGG	+	chr7.1:34725906-34725925	MS.gene93658:CDS	25.0%
!!	TTCTTCTTGATTGATTTCTT+AGG	-	chr7.1:34725681-34725700	None:intergenic	25.0%
	AAACTTCCAACTTGAAAATG+CGG	-	chr7.1:34726042-34726061	None:intergenic	30.0%
	AAGAAGAAACCGAAAAAAGA+AGG	+	chr7.1:34725693-34725712	MS.gene93658:CDS	30.0%
	AATCTTTAATGTGGATATCC+AGG	-	chr7.1:34725629-34725648	None:intergenic	30.0%
	ATAACGATGATTGTTTGAAG+TGG	+	chr7.1:34725853-34725872	MS.gene93658:CDS	30.0%
	CTAGAAGCTAAAAAGATCTT+GGG	-	chr7.1:34725738-34725757	None:intergenic	30.0%
	GAATGCTTAAAATTCAAGTC+CGG	+	chr7.1:34726014-34726033	MS.gene93658:CDS	30.0%
	GACTTCAATGATTCTATCAA+GGG	+	chr7.1:34725576-34725595	MS.gene93658:CDS	30.0%
	TAGAATCATTGAAGTCACTT+AGG	-	chr7.1:34725572-34725591	None:intergenic	30.0%
	TGACTTCAATGATTCTATCA+AGG	+	chr7.1:34725575-34725594	MS.gene93658:CDS	30.0%
!	ATAATTCTTTTTCCATCACG+TGG	-	chr7.1:34725465-34725484	None:intergenic	30.0%
!	TTCTTCTTTACCATTTTGTG+GGG	-	chr7.1:34725517-34725536	None:intergenic	30.0%
!!	GTTTTAGATAAAGAAGGCAT+CGG	-	chr7.1:34725897-34725916	None:intergenic	30.0%
!!!	AAGATCTTTTTAGCTTCTAG+GGG	+	chr7.1:34725738-34725757	MS.gene93658:CDS	30.0%
!!!	ATATCCATGCCTTCTTTTTT+CGG	-	chr7.1:34725705-34725724	None:intergenic	30.0%
!!!	CAAGATCTTTTTAGCTTCTA+GGG	+	chr7.1:34725737-34725756	MS.gene93658:CDS	30.0%
!!!	GCATTGGTTTTAGATAAAGA+AGG	-	chr7.1:34725903-34725922	None:intergenic	30.0%
	AAAATGTCCAAGAACGAAGA+AGG	+	chr7.1:34725409-34725428	MS.gene93658:CDS	35.0%
	AATCCTGAATCAGTTGATTG+CGG	+	chr7.1:34725966-34725985	MS.gene93658:CDS	35.0%
	AATGATTCTATCAAGGGATC+GGG	+	chr7.1:34725582-34725601	MS.gene93658:CDS	35.0%
	CAATGATTCTATCAAGGGAT+CGG	+	chr7.1:34725581-34725600	MS.gene93658:CDS	35.0%
	CCTAGAAGCTAAAAAGATCT+TGG	-	chr7.1:34725739-34725758	None:intergenic	35.0%
	GATTGCGGTCAAAAACATAT+TGG	+	chr7.1:34725981-34726000	MS.gene93658:CDS	35.0%
!	GATGATTGTTTGAAGTGGTT+GGG	+	chr7.1:34725858-34725877	MS.gene93658:CDS	35.0%
!	TCTTCTTTACCATTTTGTGG+GGG	-	chr7.1:34725516-34725535	None:intergenic	35.0%
!	TTCATCGATACCTTTTGCAT+TGG	-	chr7.1:34725919-34725938	None:intergenic	35.0%
!!	AATGCAAAAGGTATCGATGA+AGG	+	chr7.1:34725918-34725937	MS.gene93658:CDS	35.0%
!!!	CCAAGATCTTTTTAGCTTCT+AGG	+	chr7.1:34725736-34725755	MS.gene93658:CDS	35.0%
	GAAACCGAAAAAAGAAGGCA+TGG	+	chr7.1:34725698-34725717	MS.gene93658:CDS	40.0%
	TATGATTTCCCCCCACAAAA+TGG	+	chr7.1:34725504-34725523	MS.gene93658:CDS	40.0%
	TCCAGGTTCACTTGATTCAT+AGG	-	chr7.1:34725612-34725631	None:intergenic	40.0%
	TCCTATGAATCAAGTGAACC+TGG	+	chr7.1:34725608-34725627	MS.gene93658:CDS	40.0%
!	CGATGATTGTTTGAAGTGGT+TGG	+	chr7.1:34725857-34725876	MS.gene93658:CDS	40.0%
!	CTTCTTTACCATTTTGTGGG+GGG	-	chr7.1:34725515-34725534	None:intergenic	40.0%
!!	CAAAAGGTATCGATGAAGGT+AGG	+	chr7.1:34725922-34725941	MS.gene93658:CDS	40.0%
!!	GAATAACTGGTCTTGGAAGA+TGG	-	chr7.1:34725767-34725786	None:intergenic	40.0%
	CCAACTTGAAAATGCGGTAC+CGG	-	chr7.1:34726036-34726055	None:intergenic	45.0%
	CCAAGACCAGTTATTCCCAT+CGG	+	chr7.1:34725771-34725790	MS.gene93658:CDS	45.0%
	TCAAGCGGAAGTTCCCATAT+GGG	+	chr7.1:34725803-34725822	MS.gene93658:CDS	45.0%
	TTCAAGCGGAAGTTCCCATA+TGG	+	chr7.1:34725802-34725821	MS.gene93658:CDS	45.0%
!	CTTTTTCCATCACGTGGTGA+TGG	-	chr7.1:34725459-34725478	None:intergenic	45.0%
!	TGACCGCAATCAACTGATTC+AGG	-	chr7.1:34725972-34725991	None:intergenic	45.0%
!!	AAGAAGGCATCGGCCAAATT+TGG	-	chr7.1:34725887-34725906	None:intergenic	45.0%
!!	AGAAGGCATCGGCCAAATTT+GGG	-	chr7.1:34725886-34725905	None:intergenic	45.0%
!!!	TTGGTTTTGGCCTCCCATAT+GGG	-	chr7.1:34725819-34725838	None:intergenic	45.0%
	AGTGGTTGGGCACCCAAATT+TGG	+	chr7.1:34725871-34725890	MS.gene93658:CDS	50.0%
	CAGTTATTCCCATCGGACCT+AGG	+	chr7.1:34725778-34725797	MS.gene93658:CDS	50.0%
	CATCGGACCTAGGTTTCAAG+CGG	+	chr7.1:34725788-34725807	MS.gene93658:CDS	50.0%
	CTAGGTCCGATGGGAATAAC+TGG	-	chr7.1:34725780-34725799	None:intergenic	50.0%
	GACATGAGAGGCCAAGAGTT+GGG	-	chr7.1:34725437-34725456	None:intergenic	50.0%
	GCTTGAAACCTAGGTCCGAT+GGG	-	chr7.1:34725789-34725808	None:intergenic	50.0%
	GGAACTTCCGCTTGAAACCT+AGG	-	chr7.1:34725798-34725817	None:intergenic	50.0%
	TCATGTCCATCACCACGTGA+TGG	+	chr7.1:34725450-34725469	MS.gene93658:CDS	50.0%
	TTGGGTGCCTTCTTCGTTCT+TGG	-	chr7.1:34725419-34725438	None:intergenic	50.0%
!	CCGATGGGAATAACTGGTCT+TGG	-	chr7.1:34725774-34725793	None:intergenic	50.0%
!!!	CCGGTACCGCATTTTCAAGT+TGG	+	chr7.1:34726033-34726052	MS.gene93658:CDS	50.0%
!!!	GTTGGTTTTGGCCTCCCATA+TGG	-	chr7.1:34725820-34725839	None:intergenic	50.0%
	AGCGGAAGTTCCCATATGGG+AGG	+	chr7.1:34725806-34725825	MS.gene93658:CDS	55.0%
	CGCTTGAAACCTAGGTCCGA+TGG	-	chr7.1:34725790-34725809	None:intergenic	55.0%
	GGACATGAGAGGCCAAGAGT+TGG	-	chr7.1:34725438-34725457	None:intergenic	55.0%
!!	CACGTGGTGATGGACATGAG+AGG	-	chr7.1:34725449-34725468	None:intergenic	55.0%
!!	CGAAGAAGGCACCCAACTCT+TGG	+	chr7.1:34725423-34725442	MS.gene93658:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	34725393	34726073	34725393	ID=MS.gene93658
chr7.1	mRNA	34725393	34726073	34725393	ID=MS.gene93658.t1;Parent=MS.gene93658
chr7.1	exon	34725393	34726073	34725393	ID=MS.gene93658.t1.exon1;Parent=MS.gene93658.t1
chr7.1	CDS	34725393	34726073	34725393	ID=cds.MS.gene93658.t1;Parent=MS.gene93658.t1

Gene Sequence

>MS.gene93658

ATGAAACATGGTTCTGAAAATGTCCAAGAACGAAGAAGGCACCCAACTCTTGGCCTCTCATGTCCATCACCACGTGATGGAAAAAGAATTATTGTACAAGGAGTACAAAAATATGATTTCCCCCCACAAAATGGTAAAGAAGAATTTTGTGGTATTTTTACACAAGATTATTCTCACCTAAGTGACTTCAATGATTCTATCAAGGGATCGGGTTATCCTATGAATCAAGTGAACCTGGATATCCACATTAAAGATTTAATGGATGACAATAGTTATTATTGTAGTCCTAAGAAATCAATCAAGAAGAAACCGAAAAAAGAAGGCATGGATATCAATCACTCACCCAAGATCTTTTTAGCTTCTAGGGGTAACCATCTTCCAAGACCAGTTATTCCCATCGGACCTAGGTTTCAAGCGGAAGTTCCCATATGGGAGGCCAAAACCAACATAAATAAATCTAATAACGATGATTGTTTGAAGTGGTTGGGCACCCAAATTTGGCCGATGCCTTCTTTATCTAAAACCAATGCAAAAGGTATCGATGAAGGTAGGCTTGACTCATGCTCGTCTGACAATCCTGAATCAGTTGATTGCGGTCAAAAACATATTGGCGAAGCTAGAGAATGCTTAAAATTCAAGTCCGGTACCGCATTTTCAAGTTGGAAGTTTGATGACATGTGA

Protein sequence

>MS.gene93658.t1

MKHGSENVQERRRHPTLGLSCPSPRDGKRIIVQGVQKYDFPPQNGKEEFCGIFTQDYSHLSDFNDSIKGSGYPMNQVNLDIHIKDLMDDNSYYCSPKKSIKKKPKKEGMDINHSPKIFLASRGNHLPRPVIPIGPRFQAEVPIWEAKTNINKSNNDDCLKWLGTQIWPMPSLSKTNAKGIDEGRLDSCSSDNPESVDCGQKHIGEARECLKFKSGTAFSSWKFDDM