AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93899

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93899.t1 MTR_4g033085 96.815 314 10 0 1 314 1 314 0.0 625
MS.gene93899.t1 MTR_4g033170 96.178 314 12 0 1 314 1 314 0.0 622
MS.gene93899.t1 MTR_4g033200 76.415 318 69 1 1 312 1 318 0.0 510
MS.gene93899.t1 MTR_4g088200 76.972 317 69 1 1 313 1 317 0.0 508
MS.gene93899.t1 MTR_4g033015 76.101 318 70 1 1 312 1 318 0.0 507
MS.gene93899.t1 MTR_4g033150 73.899 318 77 1 1 312 1 318 2.02e-177 495
MS.gene93899.t1 MTR_4g088190 74.455 321 75 1 1 314 1 321 1.91e-170 478
MS.gene93899.t1 MTR_0794s0020 73.092 249 61 1 1 243 1 249 1.40e-136 387
MS.gene93899.t1 MTR_7g014570 49.045 314 156 3 6 319 6 315 2.29e-106 315
MS.gene93899.t1 MTR_7g014510 46.541 318 163 3 6 319 6 320 2.71e-101 302
MS.gene93899.t1 MTR_1g076940 47.678 323 158 5 5 319 5 324 6.91e-99 296
MS.gene93899.t1 MTR_7g024500 45.687 313 162 4 6 317 5 310 9.28e-97 290
MS.gene93899.t1 MTR_4g088345 48.418 316 158 4 1 314 1 313 4.86e-95 286
MS.gene93899.t1 MTR_7g024520 45.367 313 163 4 6 317 5 310 2.88e-94 284
MS.gene93899.t1 MTR_5g074680 44.201 319 167 6 3 313 1 316 2.89e-93 282
MS.gene93899.t1 MTR_5g074600 44.728 313 165 5 11 318 11 320 7.31e-93 281
MS.gene93899.t1 MTR_5g074580 43.175 315 171 5 4 313 5 316 4.71e-88 268
MS.gene93899.t1 MTR_5g074540 41.311 305 159 5 11 313 11 297 1.22e-84 259
MS.gene93899.t1 MTR_5g074610 42.810 306 142 3 11 313 7 282 2.64e-84 258
MS.gene93899.t1 MTR_4g088340 47.099 293 150 4 24 314 1 290 6.65e-84 257
MS.gene93899.t1 MTR_4g079160 41.776 304 174 2 11 313 10 311 5.62e-83 255
MS.gene93899.t1 MTR_1g076930 45.833 240 121 5 24 257 1 237 4.24e-59 190
MS.gene93899.t1 MTR_3g083620 35.644 303 181 9 20 317 27 320 5.55e-48 165
MS.gene93899.t1 MTR_0017s0100 33.121 314 203 6 3 313 14 323 8.00e-46 159
MS.gene93899.t1 MTR_4g038440 32.803 314 204 6 3 313 14 323 5.68e-45 157
MS.gene93899.t1 MTR_0079s0070 32.990 291 188 6 26 313 3 289 1.17e-40 145
MS.gene93899.t1 MTR_5g098170 34.211 266 157 9 31 284 33 292 1.28e-34 130
MS.gene93899.t1 MTR_0591s0010 29.893 281 179 7 26 295 41 314 1.26e-32 125
MS.gene93899.t1 MTR_7g118320 31.111 270 171 6 26 284 26 291 8.87e-32 122
MS.gene93899.t1 MTR_1g096310 29.688 320 203 8 5 312 17 326 1.19e-31 122
MS.gene93899.t1 MTR_7g118300 30.545 275 176 7 26 292 42 309 1.38e-31 122
MS.gene93899.t1 MTR_8g068870 28.094 299 197 7 5 292 6 297 2.46e-31 121
MS.gene93899.t1 MTR_3g092900 30.841 321 185 10 9 312 17 317 2.47e-31 121
MS.gene93899.t1 MTR_2g072790 32.990 291 178 8 31 313 27 308 1.33e-30 119
MS.gene93899.t1 MTR_8g068690 29.537 281 180 7 26 295 40 313 1.59e-30 119
MS.gene93899.t1 MTR_7g012090 29.242 277 173 7 32 292 40 309 3.23e-30 118
MS.gene93899.t1 MTR_7g011900 29.021 286 187 7 32 307 40 319 6.08e-30 117
MS.gene93899.t1 MTR_8g068750 28.920 287 186 7 26 301 41 320 9.10e-30 117
MS.gene93899.t1 MTR_7g012040 28.519 270 178 7 32 292 39 302 2.63e-29 115
MS.gene93899.t1 MTR_1g036460 29.492 295 190 7 24 306 29 317 4.11e-29 115
MS.gene93899.t1 MTR_7g012070 28.986 276 175 7 32 292 40 309 4.47e-29 115
MS.gene93899.t1 MTR_1g036490 29.153 295 191 7 24 306 29 317 7.47e-29 114
MS.gene93899.t1 MTR_4g038440 30.620 258 175 4 3 257 14 270 9.74e-29 112
MS.gene93899.t1 MTR_7g011990 26.619 278 187 7 32 300 40 309 7.48e-28 111
MS.gene93899.t1 MTR_8g068570 30.000 280 153 8 26 295 41 287 9.71e-28 110
MS.gene93899.t1 MTR_7g012020 29.545 264 171 7 32 284 39 298 1.07e-27 111
MS.gene93899.t1 MTR_2g055940 29.856 278 168 9 32 294 34 299 5.00e-27 109
MS.gene93899.t1 MTR_3g021440 30.584 291 183 9 28 305 38 322 4.55e-26 107
MS.gene93899.t1 MTR_8g024160 32.195 205 131 4 89 292 65 262 6.58e-26 105
MS.gene93899.t1 MTR_1g036510 28.620 297 188 10 25 306 44 331 5.58e-24 101
MS.gene93899.t1 MTR_6g078850 26.298 289 191 7 9 284 33 312 2.90e-23 100
MS.gene93899.t1 MTR_3g021430 29.252 294 185 10 32 312 29 312 3.23e-22 95.9
MS.gene93899.t1 MTR_3g096050 27.181 298 201 10 23 313 24 312 7.22e-21 92.4
MS.gene93899.t1 MTR_7g012020 28.216 241 158 7 32 261 39 275 1.02e-20 90.9
MS.gene93899.t1 MTR_8g068730 28.519 270 146 7 26 284 41 274 1.86e-20 90.9
MS.gene93899.t1 MTR_3g096070 25.676 296 185 9 23 313 24 289 1.16e-18 85.5
MS.gene93899.t1 MTR_8g068690 26.016 246 167 6 26 260 40 281 2.27e-18 84.0
MS.gene93899.t1 MTR_8g068740 28.571 273 148 8 26 287 41 277 5.88e-17 80.1
MS.gene93899.t1 MTR_8g068600 25.862 232 157 6 38 258 27 254 5.98e-17 80.1
MS.gene93899.t1 MTR_1g036510 28.226 248 157 9 25 257 44 285 1.04e-14 73.9
MS.gene93899.t1 MTR_4g025570 39.796 98 51 3 6 101 16 107 3.10e-14 71.2
MS.gene93899.t1 MTR_8g068620 33.929 112 72 1 149 260 4 113 3.64e-14 68.6
MS.gene93899.t1 MTR_7g012020 28.095 210 136 7 32 230 39 244 7.57e-13 67.8
MS.gene93899.t1 MTR_1g050220 33.613 119 66 2 188 306 147 252 2.42e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93899.t1 AT4G35150 30.645 310 158 8 16 313 15 279 7.65e-37 135
MS.gene93899.t1 AT5G54160 32.180 289 182 6 31 312 34 315 8.92e-36 133
MS.gene93899.t1 AT4G35160 32.500 320 190 10 16 317 29 340 1.03e-34 130
MS.gene93899.t1 AT1G77520 33.094 278 163 7 31 292 44 314 1.66e-32 124
MS.gene93899.t1 AT3G53140 31.987 297 178 10 31 313 27 313 9.60e-31 119
MS.gene93899.t1 AT5G53810 31.560 282 169 8 29 293 38 312 1.40e-26 108
MS.gene93899.t1 AT1G63140 29.545 308 191 9 9 297 19 319 2.19e-26 108
MS.gene93899.t1 AT1G33030 27.645 293 194 8 31 313 21 305 2.33e-26 107
MS.gene93899.t1 AT1G63140 29.545 308 191 9 9 297 39 339 2.44e-26 108
MS.gene93899.t1 AT1G51990 28.968 252 162 6 64 309 67 307 4.56e-25 103
MS.gene93899.t1 AT1G77530 31.900 279 167 7 31 293 44 315 1.71e-24 102
MS.gene93899.t1 AT1G51990 28.968 252 162 6 64 309 67 307 5.09e-24 101
MS.gene93899.t1 AT1G76790 26.769 325 205 10 7 313 9 318 3.72e-21 93.2
MS.gene93899.t1 AT1G62900 36.641 131 80 1 167 297 16 143 2.92e-19 85.1
MS.gene93899.t1 AT1G21130 25.418 299 195 9 31 313 40 326 4.41e-16 78.6
MS.gene93899.t1 AT1G21110 24.749 299 197 9 31 313 40 326 7.20e-16 78.2
MS.gene93899.t1 AT1G21120 25.084 299 196 9 31 313 40 326 7.55e-16 78.2
MS.gene93899.t1 AT5G37170 30.000 150 100 3 166 313 43 189 7.78e-16 76.3
MS.gene93899.t1 AT1G21120 25.084 299 196 9 31 313 88 374 1.17e-15 77.8
MS.gene93899.t1 AT1G21100 24.749 299 197 8 31 313 40 326 1.17e-15 77.4
MS.gene93899.t1 AT5G37170 30.000 150 100 3 166 313 143 289 1.20e-15 77.0
MS.gene93899.t1 AT5G37170 32.824 131 80 4 188 313 14 141 5.22e-15 72.8
MS.gene93899.t1 AT1G63140 27.239 268 172 8 9 257 39 302 6.46e-14 71.6
MS.gene93899.t1 AT1G33030 26.160 237 160 7 31 257 21 252 1.19e-13 70.5
MS.gene93899.t1 AT1G33030 26.160 237 160 7 31 257 21 252 1.99e-13 69.7

Find 65 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTATACATGTTTGTATAAA+TGG 0.173388 4.4:-70296244 None:intergenic
ACTGTTCTTCAATATCTTTA+AGG 0.222796 4.4:-70297843 None:intergenic
TATAGGACACTAACTTTAGA+AGG 0.276793 4.4:-70296383 None:intergenic
TTCAAAGATGAGGATAATTC+AGG 0.281306 4.4:-70296353 None:intergenic
CAGCCATGTGTTTGAGGGTT+TGG 0.284838 4.4:+70296748 MS.gene93899:CDS
GTTTGAGGGTTTGGAGTCTT+TGG 0.303653 4.4:+70296757 MS.gene93899:CDS
CTCAGAGAACCATTTCTTAG+AGG 0.305502 4.4:-70296579 None:intergenic
GTTGATGTGGCAGGTGGGAC+TGG 0.337992 4.4:+70296779 MS.gene93899:CDS
CTAAAGTTAGTGTCCTATAT+AGG 0.347448 4.4:+70296387 MS.gene93899:CDS
ACTAACAAGTGGTGCAAAAC+AGG 0.393012 4.4:-70296519 None:intergenic
AAAGAAGCAATTTCAAGAAA+AGG 0.393418 4.4:+70297866 MS.gene93899:CDS
CCACTGATGAGAAGCTTTGA+AGG 0.393766 4.4:-70296488 None:intergenic
CAGGTTGTGGCTAACTTATC+TGG 0.396383 4.4:+70296866 MS.gene93899:CDS
GAGAGACTTGAAGCCGCATA+TGG 0.397843 4.4:-70298074 None:intergenic
TCATAGGCTTTCCATGTTTG+TGG 0.399025 4.4:-70296325 None:intergenic
GCACTTTATGAAAGTGCAAC+GGG 0.400827 4.4:+70296614 MS.gene93899:CDS
GATAAACTTAGTTTGTTTCA+AGG 0.401061 4.4:+70296677 MS.gene93899:CDS
GATAATTCAGGAACAGTCAT+AGG 0.424086 4.4:-70296341 None:intergenic
CAAAATTTGAACTTTGTTGG+TGG 0.427047 4.4:+70296893 MS.gene93899:CDS
ACTCAAAATTTGAACTTTGT+TGG 0.429435 4.4:+70296890 MS.gene93899:CDS
AAGAAGCAATTTCAAGAAAA+GGG 0.443928 4.4:+70297867 MS.gene93899:CDS
AATGTTCAAGCTTGCTCTCA+AGG 0.450410 4.4:+70296721 MS.gene93899:CDS
TGTGGCACTCCTCTAAGAAA+TGG 0.452397 4.4:+70296570 MS.gene93899:CDS
GATTGCAGCCATGTGTTTGA+GGG 0.455193 4.4:+70296743 MS.gene93899:CDS
ATAAACTTAGTTTGTTTCAA+GGG 0.456335 4.4:+70296678 MS.gene93899:CDS
AGTGCTTCATCCATGTTCTC+TGG 0.459421 4.4:+70296544 MS.gene93899:CDS
GCAAGCGCAAGAACCTATAT+AGG 0.464145 4.4:-70296400 None:intergenic
GGATTGCAGCCATGTGTTTG+AGG 0.478258 4.4:+70296742 MS.gene93899:CDS
GAGTCTTTGGTTGATGTGGC+AGG 0.479885 4.4:+70296770 MS.gene93899:CDS
ATCCATGTTCTCTGGATATG+TGG 0.500274 4.4:+70296552 MS.gene93899:CDS
TCTTTGGTTGATGTGGCAGG+TGG 0.504535 4.4:+70296773 MS.gene93899:CDS
CTCAAATCAGCTGTGGAACT+AGG 0.506689 4.4:+70296287 MS.gene93899:CDS
TTTAGATCAACCACAGGTTG+TGG 0.518679 4.4:+70296853 MS.gene93899:CDS
CACAGCTGATTTGAGTGCCA+TGG 0.520024 4.4:-70296279 None:intergenic
GAAATGGTTCTCTGAGGAGA+AGG 0.549990 4.4:+70296586 MS.gene93899:CDS
TGCACTTTATGAAAGTGCAA+CGG 0.553435 4.4:+70296613 MS.gene93899:CDS
TTTGGAGTCTTTGGTTGATG+TGG 0.555368 4.4:+70296766 MS.gene93899:CDS
TTGAGTAAAACAGCATCTGC+AGG 0.557051 4.4:-70296938 None:intergenic
TAAACTTAGTTTGTTTCAAG+GGG 0.566712 4.4:+70296679 MS.gene93899:CDS
AGGGTGAAGTTTCAAAGATG+AGG 0.567354 4.4:-70296363 None:intergenic
AGAGACTTGAAGCCGCATAT+GGG 0.571433 4.4:-70298073 None:intergenic
ATACAGCTTTATAAGTTCCA+TGG 0.573273 4.4:+70296262 MS.gene93899:CDS
ACAATGGATTTCAAGAGCAA+TGG 0.576228 4.4:+70296188 None:intergenic
CTTTGGTTGATGTGGCAGGT+GGG 0.578821 4.4:+70296774 MS.gene93899:CDS
GTTGCAATTGCACTTTGACA+TGG 0.578956 4.4:+70297958 MS.gene93899:CDS
CCTTCAAAGCTTCTCATCAG+TGG 0.586184 4.4:+70296488 MS.gene93899:CDS
TGCCACATATCCAGAGAACA+TGG 0.593078 4.4:-70296554 None:intergenic
CATTTGTGAATCAGCTGCCA+TGG 0.594995 4.4:-70296702 None:intergenic
GTCAAAGTGCAATTGCAACT+CGG 0.597693 4.4:-70297954 None:intergenic
TAGTTTGTTTCAAGGGGCCA+TGG 0.599443 4.4:+70296685 MS.gene93899:CDS
GATAAGTTAGCCACAACCTG+TGG 0.609880 4.4:-70296863 None:intergenic
AGTAAAACAGCATCTGCAGG+AGG 0.624714 4.4:-70296935 None:intergenic
TATAAGATTACTCCCATATG+CGG 0.627092 4.4:+70298061 MS.gene93899:CDS
GTAGATGCAATCCACAAACA+TGG 0.627357 4.4:+70296314 MS.gene93899:CDS
ATAGGACACTAACTTTAGAA+GGG 0.629455 4.4:-70296382 None:intergenic
CATGGCACTCAAATCAGCTG+TGG 0.632130 4.4:+70296280 MS.gene93899:CDS
ACTCCAAACCCTCAAACACA+TGG 0.632186 4.4:-70296751 None:intergenic
CACTTTATGAAAGTGCAACG+GGG 0.648505 4.4:+70296615 MS.gene93899:CDS
TTGCGCTTGCTAACACACGA+TGG 0.651331 4.4:+70296413 MS.gene93899:CDS
AAAACTGTGCATTTGATGTG+AGG 0.654544 4.4:-70296833 None:intergenic
TGAAGCACTCCACTAACAAG+TGG 0.666600 4.4:-70296530 None:intergenic
CTCTAAGAAATGGTTCTCTG+AGG 0.677453 4.4:+70296580 MS.gene93899:CDS
ACTTTATGAAAGTGCAACGG+GGG 0.680144 4.4:+70296616 MS.gene93899:CDS
GTAAAACAGCATCTGCAGGA+GGG 0.681270 4.4:-70296934 None:intergenic
CTGATGAGAAGCTTTGAAGG+AGG 0.702373 4.4:-70296485 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATATTATTTTAATTTAT+TGG + chr4.4:70297740-70297759 MS.gene93899:intron 0.0%
!! AAAATTCATATTTAATTCAT+TGG - chr4.4:70297433-70297452 None:intergenic 10.0%
!! ATTTCATTAAAAAAATATGA+AGG + chr4.4:70297558-70297577 MS.gene93899:intron 10.0%
!!! AATATGAATTTTTATGTTTT+TGG + chr4.4:70297441-70297460 MS.gene93899:intron 10.0%
!!! ATTTTACAATTTTATTTCTT+TGG - chr4.4:70297722-70297741 None:intergenic 10.0%
!!! TTTTACAATTTTATTTCTTT+GGG - chr4.4:70297721-70297740 None:intergenic 10.0%
!! TTTGTTAAGAAAGTTAAAAA+AGG - chr4.4:70296645-70296664 None:intergenic 15.0%
!! ACAAATTTATATCTTTGATC+AGG - chr4.4:70297386-70297405 None:intergenic 20.0%
!! AGGTGATAAAATAATAACAT+CGG + chr4.4:70297123-70297142 MS.gene93899:intron 20.0%
!! ATCCAAAAAAAAATTACTTG+TGG + chr4.4:70297050-70297069 MS.gene93899:intron 20.0%
!! CAAAATAAAATGGAATCAAA+TGG - chr4.4:70296990-70297009 None:intergenic 20.0%
!! TATGTTCTGATTTCATAATT+AGG + chr4.4:70297016-70297035 MS.gene93899:intron 20.0%
!! TCCAAAAAAAAATTACTTGT+GGG + chr4.4:70297051-70297070 MS.gene93899:intron 20.0%
!! TGATCAAAGATATAAATTTG+TGG + chr4.4:70297385-70297404 MS.gene93899:intron 20.0%
!! TGTATACATGTTTGTATAAA+TGG - chr4.4:70296247-70296266 None:intergenic 20.0%
!!! ACGTAAGAACAATTTTTATT+TGG - chr4.4:70297258-70297277 None:intergenic 20.0%
!!! ATAAACTTAGTTTGTTTCAA+GGG + chr4.4:70296678-70296697 MS.gene93899:CDS 20.0%
!!! TCATAGATGAAAAATTTTGA+GGG + chr4.4:70297618-70297637 MS.gene93899:intron 20.0%
!!! TTCATAGATGAAAAATTTTG+AGG + chr4.4:70297617-70297636 MS.gene93899:intron 20.0%
! AAAGAAGCAATTTCAAGAAA+AGG + chr4.4:70297866-70297885 MS.gene93899:CDS 25.0%
! AAGAAAGAGAAAAGAAAGAA+TGG + chr4.4:70298005-70298024 MS.gene93899:intron 25.0%
! AAGAAGCAATTTCAAGAAAA+GGG + chr4.4:70297867-70297886 MS.gene93899:CDS 25.0%
! AATCAAATGTTGGTACATTT+AGG + chr4.4:70297675-70297694 MS.gene93899:intron 25.0%
! ACTCAAAATTTGAACTTTGT+TGG + chr4.4:70296890-70296909 MS.gene93899:CDS 25.0%
! ACTGTTCTTCAATATCTTTA+AGG - chr4.4:70297846-70297865 None:intergenic 25.0%
! ATCAAATGTTGGTACATTTA+GGG + chr4.4:70297676-70297695 MS.gene93899:intron 25.0%
! ATTATGAAATCAGAACATAC+AGG - chr4.4:70297015-70297034 None:intergenic 25.0%
! ATTTCAAGAAAAGGGAAAAA+AGG + chr4.4:70297875-70297894 MS.gene93899:CDS 25.0%
! CAAAAAAAAATTACTTGTGG+GGG + chr4.4:70297053-70297072 MS.gene93899:intron 25.0%
! CCAAAAAAAAATTACTTGTG+GGG + chr4.4:70297052-70297071 MS.gene93899:intron 25.0%
! TTATGAAATCAGAACATACA+GGG - chr4.4:70297014-70297033 None:intergenic 25.0%
!! CATAATTTTTACCAATGTGT+AGG + chr4.4:70297103-70297122 MS.gene93899:intron 25.0%
!!! AAGCCTAAGAAATATTTTAG+AGG + chr4.4:70297646-70297665 MS.gene93899:intron 25.0%
!!! AGCCTAAGAAATATTTTAGA+GGG + chr4.4:70297647-70297666 MS.gene93899:intron 25.0%
!!! ATAAGTTTTGACTAAAACGT+AGG + chr4.4:70297587-70297606 MS.gene93899:intron 25.0%
!!! GATAAACTTAGTTTGTTTCA+AGG + chr4.4:70296677-70296696 MS.gene93899:CDS 25.0%
!!! TAAACTTAGTTTGTTTCAAG+GGG + chr4.4:70296679-70296698 MS.gene93899:CDS 25.0%
!!! TAAGTTTTGACTAAAACGTA+GGG + chr4.4:70297588-70297607 MS.gene93899:intron 25.0%
!!! TTATTTTATCACCTACACAT+TGG - chr4.4:70297117-70297136 None:intergenic 25.0%
AAGAAACTCATATATGATGC+AGG + chr4.4:70298028-70298047 MS.gene93899:intron 30.0%
ATCTCAAATGTCTTTCAAAG+TGG + chr4.4:70297792-70297811 MS.gene93899:intron 30.0%
CAAAATTTGAACTTTGTTGG+TGG + chr4.4:70296893-70296912 MS.gene93899:CDS 30.0%
CATACAGGGACAAAATAAAA+TGG - chr4.4:70297000-70297019 None:intergenic 30.0%
CATACATAGAGAGATATTTC+CGG - chr4.4:70297333-70297352 None:intergenic 30.0%
CTAAAGTTAGTGTCCTATAT+AGG + chr4.4:70296387-70296406 MS.gene93899:CDS 30.0%
TATAAGATTACTCCCATATG+CGG + chr4.4:70298061-70298080 MS.gene93899:CDS 30.0%
TATAGGACACTAACTTTAGA+AGG - chr4.4:70296386-70296405 None:intergenic 30.0%
TCAAATGTTGGTACATTTAG+GGG + chr4.4:70297677-70297696 MS.gene93899:intron 30.0%
TCTCAAATGTCTTTCAAAGT+GGG + chr4.4:70297793-70297812 MS.gene93899:intron 30.0%
TTCAAAGATGAGGATAATTC+AGG - chr4.4:70296356-70296375 None:intergenic 30.0%
TTTATATCTTTGATCAGGCA+CGG - chr4.4:70297381-70297400 None:intergenic 30.0%
! ATACAGCTTTATAAGTTCCA+TGG + chr4.4:70296262-70296281 MS.gene93899:CDS 30.0%
! ATAGGACACTAACTTTAGAA+GGG - chr4.4:70296385-70296404 None:intergenic 30.0%
! CTTTGGGTTAAATACGTAAA+TGG - chr4.4:70297705-70297724 None:intergenic 30.0%
!! GTCCCTCTAAAATATTTCTT+AGG - chr4.4:70297652-70297671 None:intergenic 30.0%
!!! CCCCACAAGTAATTTTTTTT+TGG - chr4.4:70297055-70297074 None:intergenic 30.0%
!!! TTGCTGATTTTTAACACTAC+CGG + chr4.4:70297311-70297330 MS.gene93899:intron 30.0%
AAAACTGTGCATTTGATGTG+AGG - chr4.4:70296836-70296855 None:intergenic 35.0%
CAAATGTTGGTACATTTAGG+GGG + chr4.4:70297678-70297697 MS.gene93899:intron 35.0%
GAGGGACTAAAATCAAATGT+TGG + chr4.4:70297665-70297684 MS.gene93899:intron 35.0%
GATAATTCAGGAACAGTCAT+AGG - chr4.4:70296344-70296363 None:intergenic 35.0%
GTAATGATGACTCTGCATAA+TGG + chr4.4:70297980-70297999 MS.gene93899:intron 35.0%
! TGCACTTTATGAAAGTGCAA+CGG + chr4.4:70296613-70296632 MS.gene93899:CDS 35.0%
!! AAAGTGGGTTTTGCATGATT+GGG + chr4.4:70297808-70297827 MS.gene93899:intron 35.0%
ACTAACAAGTGGTGCAAAAC+AGG - chr4.4:70296522-70296541 None:intergenic 40.0%
AGGGTGAAGTTTCAAAGATG+AGG - chr4.4:70296366-70296385 None:intergenic 40.0%
ATCCACTCAGATGAGATTTG+GGG - chr4.4:70297358-70297377 None:intergenic 40.0%
ATCCATGTTCTCTGGATATG+TGG + chr4.4:70296552-70296571 MS.gene93899:CDS 40.0%
CTCAGAGAACCATTTCTTAG+AGG - chr4.4:70296582-70296601 None:intergenic 40.0%
GATCCACTCAGATGAGATTT+GGG - chr4.4:70297359-70297378 None:intergenic 40.0%
GTAGATGCAATCCACAAACA+TGG + chr4.4:70296314-70296333 MS.gene93899:CDS 40.0%
GTCAAAGTGCAATTGCAACT+CGG - chr4.4:70297957-70297976 None:intergenic 40.0%
GTTGCAATTGCACTTTGACA+TGG + chr4.4:70297958-70297977 MS.gene93899:CDS 40.0%
TCATAGGCTTTCCATGTTTG+TGG - chr4.4:70296328-70296347 None:intergenic 40.0%
TTGAGTAAAACAGCATCTGC+AGG - chr4.4:70296941-70296960 None:intergenic 40.0%
TTTAGATCAACCACAGGTTG+TGG + chr4.4:70296853-70296872 MS.gene93899:CDS 40.0%
! AATGTTCAAGCTTGCTCTCA+AGG + chr4.4:70296721-70296740 MS.gene93899:CDS 40.0%
! ACTTTATGAAAGTGCAACGG+GGG + chr4.4:70296616-70296635 MS.gene93899:CDS 40.0%
! CACTTTATGAAAGTGCAACG+GGG + chr4.4:70296615-70296634 MS.gene93899:CDS 40.0%
! CTCTAAGAAATGGTTCTCTG+AGG + chr4.4:70296580-70296599 MS.gene93899:CDS 40.0%
! GCACTTTATGAAAGTGCAAC+GGG + chr4.4:70296614-70296633 MS.gene93899:CDS 40.0%
! TTTGGAGTCTTTGGTTGATG+TGG + chr4.4:70296766-70296785 MS.gene93899:CDS 40.0%
!! CAAAGTGGGTTTTGCATGAT+TGG + chr4.4:70297807-70297826 MS.gene93899:intron 40.0%
!! TGTTTTGCACCACTTGTTAG+TGG + chr4.4:70296521-70296540 MS.gene93899:CDS 40.0%
!!! CACAGTTTTAGATCAACCAC+AGG + chr4.4:70296847-70296866 MS.gene93899:CDS 40.0%
!!! TGCAGATGCTGTTTTACTCA+AGG + chr4.4:70296940-70296959 MS.gene93899:CDS 40.0%
ACTCCAAACCCTCAAACACA+TGG - chr4.4:70296754-70296773 None:intergenic 45.0%
AGAGACTTGAAGCCGCATAT+GGG - chr4.4:70298076-70298095 None:intergenic 45.0%
AGTAAAACAGCATCTGCAGG+AGG - chr4.4:70296938-70296957 None:intergenic 45.0%
AGTGCTTCATCCATGTTCTC+TGG + chr4.4:70296544-70296563 MS.gene93899:CDS 45.0%
CAGGTTGTGGCTAACTTATC+TGG + chr4.4:70296866-70296885 MS.gene93899:CDS 45.0%
CATTTGTGAATCAGCTGCCA+TGG - chr4.4:70296705-70296724 None:intergenic 45.0%
CCTTCAAAGCTTCTCATCAG+TGG + chr4.4:70296488-70296507 MS.gene93899:CDS 45.0%
CTCAAATCAGCTGTGGAACT+AGG + chr4.4:70296287-70296306 MS.gene93899:CDS 45.0%
GATAAGTTAGCCACAACCTG+TGG - chr4.4:70296866-70296885 None:intergenic 45.0%
GATTGCAGCCATGTGTTTGA+GGG + chr4.4:70296743-70296762 MS.gene93899:CDS 45.0%
GCAAGCGCAAGAACCTATAT+AGG - chr4.4:70296403-70296422 None:intergenic 45.0%
GGATCCACTCAGATGAGATT+TGG - chr4.4:70297360-70297379 None:intergenic 45.0%
GTAAAACAGCATCTGCAGGA+GGG - chr4.4:70296937-70296956 None:intergenic 45.0%
TGCCACATATCCAGAGAACA+TGG - chr4.4:70296557-70296576 None:intergenic 45.0%
! CCACTGATGAGAAGCTTTGA+AGG - chr4.4:70296491-70296510 None:intergenic 45.0%
! GAAATGGTTCTCTGAGGAGA+AGG + chr4.4:70296586-70296605 MS.gene93899:CDS 45.0%
! GTTTGAGGGTTTGGAGTCTT+TGG + chr4.4:70296757-70296776 MS.gene93899:CDS 45.0%
! TGAAGCACTCCACTAACAAG+TGG - chr4.4:70296533-70296552 None:intergenic 45.0%
! TGTGGCACTCCTCTAAGAAA+TGG + chr4.4:70296570-70296589 MS.gene93899:CDS 45.0%
!! CTGATGAGAAGCTTTGAAGG+AGG - chr4.4:70296488-70296507 None:intergenic 45.0%
!! TAGTTTGTTTCAAGGGGCCA+TGG + chr4.4:70296685-70296704 MS.gene93899:CDS 45.0%
!!! ATTAATTTTTCTAAATATAA+TGG - chr4.4:70297220-70297239 None:intergenic 5.0%
CAGCCATGTGTTTGAGGGTT+TGG + chr4.4:70296748-70296767 MS.gene93899:CDS 50.0%
CATGGCACTCAAATCAGCTG+TGG + chr4.4:70296280-70296299 MS.gene93899:CDS 50.0%
GAGAGACTTGAAGCCGCATA+TGG - chr4.4:70298077-70298096 None:intergenic 50.0%
GGATTGCAGCCATGTGTTTG+AGG + chr4.4:70296742-70296761 MS.gene93899:CDS 50.0%
TGCCCCAAATCTCATCTGAG+TGG + chr4.4:70297353-70297372 MS.gene93899:intron 50.0%
! CTTTGGTTGATGTGGCAGGT+GGG + chr4.4:70296774-70296793 MS.gene93899:CDS 50.0%
! GAGTCTTTGGTTGATGTGGC+AGG + chr4.4:70296770-70296789 MS.gene93899:CDS 50.0%
! TCTTTGGTTGATGTGGCAGG+TGG + chr4.4:70296773-70296792 MS.gene93899:CDS 50.0%
! TTGCGCTTGCTAACACACGA+TGG + chr4.4:70296413-70296432 MS.gene93899:CDS 50.0%
!! CACAGCTGATTTGAGTGCCA+TGG - chr4.4:70296282-70296301 None:intergenic 50.0%
GTTGATGTGGCAGGTGGGAC+TGG + chr4.4:70296779-70296798 MS.gene93899:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 70296191 70298100 70296191 ID=MS.gene93899
chr4.4 mRNA 70296191 70298100 70296191 ID=MS.gene93899.t1;Parent=MS.gene93899
chr4.4 exon 70296191 70296961 70296191 ID=MS.gene93899.t1.exon1;Parent=MS.gene93899.t1
chr4.4 CDS 70296191 70296961 70296191 ID=cds.MS.gene93899.t1;Parent=MS.gene93899.t1
chr4.4 exon 70297812 70297979 70297812 ID=MS.gene93899.t1.exon2;Parent=MS.gene93899.t1
chr4.4 CDS 70297812 70297979 70297812 ID=cds.MS.gene93899.t1;Parent=MS.gene93899.t1
chr4.4 exon 70298050 70298100 70298050 ID=MS.gene93899.t1.exon3;Parent=MS.gene93899.t1
chr4.4 CDS 70298050 70298100 70298050 ID=cds.MS.gene93899.t1;Parent=MS.gene93899.t1
Gene Sequence

>MS.gene93899

ATGGATTTCAAGAGCAATGGCAGTGAAGAAACTCAGCTGTATCATGCTCAAATCCATTTATACAAACATGTATACAGCTTTATAAGTTCCATGGCACTCAAATCAGCTGTGGAACTAGGCATAGTAGATGCAATCCACAAACATGGAAAGCCTATGACTGTTCCTGAATTATCCTCATCTTTGAAACTTCACCCTTCTAAAGTTAGTGTCCTATATAGGTTCTTGCGCTTGCTAACACACGATGGTTTCTTTGCAAAAACAACAGTGAAAGAAGAAACAGCTTATGCTCTCACACCTCCTTCAAAGCTTCTCATCAGTGGTGAATCAACCTGTTTTGCACCACTTGTTAGTGGAGTGCTTCATCCATGTTCTCTGGATATGTGGCACTCCTCTAAGAAATGGTTCTCTGAGGAGAAGGATCTTGCACTTTATGAAAGTGCAACGGGGGAGACCTTTTTTAACTTTCTTAACAAAGATTCTGAATCTGATAAACTTAGTTTGTTTCAAGGGGCCATGGCAGCTGATTCACAAATGTTCAAGCTTGCTCTCAAGGATTGCAGCCATGTGTTTGAGGGTTTGGAGTCTTTGGTTGATGTGGCAGGTGGGACTGGAGTTGTGTCAAAACTCATTCATGAAGAATTTCCTCACATCAAATGCACAGTTTTAGATCAACCACAGGTTGTGGCTAACTTATCTGGAACTCAAAATTTGAACTTTGTTGGTGGAGATATGTTCAAATCTATCCCTCCTGCAGATGCTGTTTTACTCAAGGTACGTTTATGTTTATCAATTTTTACCATTTGATTCCATTTTATTTTGTCCCTGTATGTTCTGATTTCATAATTAGGATTAGTGACAAATCCAAAAAAAAATTACTTGTGGGGGCAAGATTTAATATAATAAATATTTATACATAATTTTTACCAATGTGTAGGTGATAAAATAATAACATCGGTTTTCTTTTTACTACTAATAAATAATAACATCATTATCTTTGAAAAATATACTTGAGATTTTAAACTCACACCATTATATTTAGAAAAATTAATGATTTTTTAAGATCTCCAAATAAAAATTGTTCTTACGTGTCTTTATATGATTAAAAAAAGTATTGTAAATTTTGCTGATTTTTAACACTACCGGAAATATCTCTCTATGTATGTGCCCCAAATCTCATCTGAGTGGATCCGTGCCTGATCAAAGATATAAATTTGTGGTAACTTTTTTAGTTAAGATATTCCAATGAATTAAATATGAATTTTTATGTTTTTGGTGTTAAAAAATTCATGTTATATTTATCTTTTGTTAAAAAATTCTATTTCTTTTTTAGAAAAATATTGTCATAATTTTCTACACATTTCTTTAAAATTTCATTAAAAAAATATGAAGGTTAGACATAAGTTTTGACTAAAACGTAGGGACTAATTTCATAGATGAAAAATTTTGAGGGATCGAAAGCCTAAGAAATATTTTAGAGGGACTAAAATCAAATGTTGGTACATTTAGGGGGACCATTTACGTATTTAACCCAAAGAAATAAAATTGTAAAATAATATTATTTTAATTTATTGGTCTATCTCATTTTGTAACATTGTACTAAAATCTCAAATGTCTTTCAAAGTGGGTTTTGCATGATTGGGATGATGAGCTCTCCTTAAAGATATTGAAGAACAGTAAAGAAGCAATTTCAAGAAAAGGGAAAAAAGGAAAGATAATAATCATAGACATATCAATTGATGAAACAAGTGATGATCGTGAGTTAACCGAGTTGCAATTGCACTTTGACATGGTAATGATGACTCTGCATAATGGAAAAGAAAGAGAAAAGAAAGAATGGAAGAAACTCATATATGATGCAGGCTTCAGCAGCTATAAGATTACTCCCATATGCGGCTTCAAGTCTCTCATAG
Protein sequence

>MS.gene93899.t1

MDFKSNGSEETQLYHAQIHLYKHVYSFISSMALKSAVELGIVDAIHKHGKPMTVPELSSSLKLHPSKVSVLYRFLRLLTHDGFFAKTTVKEETAYALTPPSKLLISGESTCFAPLVSGVLHPCSLDMWHSSKKWFSEEKDLALYESATGETFFNFLNKDSESDKLSLFQGAMAADSQMFKLALKDCSHVFEGLESLVDVAGGTGVVSKLIHEEFPHIKCTVLDQPQVVANLSGTQNLNFVGGDMFKSIPPADAVLLKWVLHDWDDELSLKILKNSKEAISRKGKKGKIIIIDISIDETSDDRELTELQLHFDMASAAIRLLPYAASSLS