Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054576 | MS.gene94013 | 0.805428 | 1.34E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 124 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTCGACAACTAATATAT+TGG | 0.248484 | 3.1:-25583888 | MS.gene94013:CDS |
| GTTTAGTTGGTGTGTCATCT+TGG | 0.308803 | 3.1:-25583950 | MS.gene94013:intron |
| TTTAGTTGGTGTGTCATCTT+GGG | 0.317491 | 3.1:-25583949 | MS.gene94013:intron |
| TAAGAATAACATTGAAGTTA+TGG | 0.344264 | 3.1:-25583106 | MS.gene94013:CDS |
| CTGATCTGTTTCTAGATGTA+TGG | 0.402326 | 3.1:+25583859 | None:intergenic |
| TATTCATTTATTGCTTGCCA+AGG | 0.479709 | 3.1:-25585287 | MS.gene94013:intron |
| AAGAAATAAAGAAGAAAAGC+GGG | 0.507920 | 3.1:+25583022 | None:intergenic |
| CATACATCTAGAAACAGATC+AGG | 0.552542 | 3.1:-25583858 | MS.gene94013:intron |
| TCCAATATATTAGTTGTCGA+AGG | 0.561283 | 3.1:+25583887 | None:intergenic |
| TTAGTTGGTGTGTCATCTTG+GGG | 0.577027 | 3.1:-25583948 | MS.gene94013:intron |
| TAAACACACTCAGATAGAGA+AGG | 0.608686 | 3.1:+25585308 | None:intergenic |
| CAAGAAATAAAGAAGAAAAG+CGG | 0.610810 | 3.1:+25583021 | None:intergenic |
| TCATCTTGGGGATATTGTTG+CGG | 0.611322 | 3.1:-25583936 | MS.gene94013:CDS |
| AAACACACTCAGATAGAGAA+GGG | 0.627458 | 3.1:+25585309 | None:intergenic |
| TCTGTTTCTAGATGTATGGA+AGG | 0.627896 | 3.1:+25583863 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTTTAAAACAATATTATT+AGG | + | chr3.1:25584736-25584755 | None:intergenic | 10.0% |
| !! | AACACAAACTTAAAATTTAA+AGG | + | chr3.1:25584514-25584533 | None:intergenic | 15.0% |
| !! | ACTTAAAACTTGAATAAATA+TGG | - | chr3.1:25584926-25584945 | MS.gene94013:intron | 15.0% |
| !! | ATTAATAGGAAAAAAACTAT+AGG | - | chr3.1:25583974-25583993 | MS.gene94013:intron | 15.0% |
| !! | GCATATATTAATGTTAAATT+AGG | + | chr3.1:25584419-25584438 | None:intergenic | 15.0% |
| !!! | AAAAGTAGTACTTTTTTTTA+AGG | - | chr3.1:25584687-25584706 | MS.gene94013:intron | 15.0% |
| !!! | ATATAATGAAGTTTTACAAA+GGG | - | chr3.1:25584856-25584875 | MS.gene94013:intron | 15.0% |
| !!! | GTTGAATAAAAGTTTTTTAA+TGG | - | chr3.1:25584888-25584907 | MS.gene94013:intron | 15.0% |
| !!! | TATATAATGAAGTTTTACAA+AGG | - | chr3.1:25584855-25584874 | MS.gene94013:intron | 15.0% |
| !!! | TGATTTTATTATACATTTAG+AGG | - | chr3.1:25585322-25585341 | MS.gene94013:CDS | 15.0% |
| !! | AAATAAGAAAAGCTAAAACA+TGG | + | chr3.1:25583910-25583929 | None:intergenic | 20.0% |
| !! | ATTATACTTTGATTAGACTT+AGG | - | chr3.1:25584103-25584122 | MS.gene94013:intron | 20.0% |
| !! | CTAATCAAAGTATAATTAAC+AGG | + | chr3.1:25584099-25584118 | None:intergenic | 20.0% |
| !! | TAAGAATAACATTGAAGTTA+TGG | - | chr3.1:25585127-25585146 | MS.gene94013:intron | 20.0% |
| !! | TACATCTTTGTCATATTATT+AGG | + | chr3.1:25584773-25584792 | None:intergenic | 20.0% |
| !! | TATTAATGTTAAATTAGGAG+AGG | + | chr3.1:25584414-25584433 | None:intergenic | 20.0% |
| !!! | GTCTTTGATTTCAAATTAAT+AGG | - | chr3.1:25583960-25583979 | MS.gene94013:intron | 20.0% |
| !!! | TATACATGATTTTGAACTAA+TGG | - | chr3.1:25583106-25583125 | MS.gene94013:CDS | 20.0% |
| ! | AAATATGCTACCAAAAAAAC+AGG | + | chr3.1:25584562-25584581 | None:intergenic | 25.0% |
| ! | AAGAAATAAAGAAGAAAAGC+GGG | + | chr3.1:25585214-25585233 | None:intergenic | 25.0% |
| ! | AAGGAAAACTAGTGAATATA+TGG | + | chr3.1:25584475-25584494 | None:intergenic | 25.0% |
| ! | AATATTGCATAGACCAAATA+AGG | + | chr3.1:25583228-25583247 | None:intergenic | 25.0% |
| ! | AATGTACAAACACAAACATA+AGG | + | chr3.1:25585026-25585045 | None:intergenic | 25.0% |
| ! | ACATTGTAAAAAAGAGAGTT+TGG | + | chr3.1:25583007-25583026 | None:intergenic | 25.0% |
| ! | AGGAAAACTAATGTACAATA+AGG | + | chr3.1:25584494-25584513 | None:intergenic | 25.0% |
| ! | AGTTCAAAATCATGTATACA+TGG | + | chr3.1:25583104-25583123 | None:intergenic | 25.0% |
| ! | ATGAACTGCATATTCTTAAA+TGG | + | chr3.1:25585187-25585206 | None:intergenic | 25.0% |
| ! | ATTTAAGAATATGCAGTTCA+TGG | - | chr3.1:25585186-25585205 | MS.gene94013:intron | 25.0% |
| ! | CAAGAAATAAAGAAGAAAAG+CGG | + | chr3.1:25585215-25585234 | None:intergenic | 25.0% |
| ! | GATACTAAGTTACTTGATAT+TGG | - | chr3.1:25585093-25585112 | MS.gene94013:intron | 25.0% |
| ! | GTAATATTGTACTTTACAGT+TGG | - | chr3.1:25585259-25585278 | MS.gene94013:intron | 25.0% |
| ! | TAAAAAAGACATGCATGTAA+TGG | - | chr3.1:25583654-25583673 | MS.gene94013:intron | 25.0% |
| ! | TAGATTTCATATACAAACCT+TGG | + | chr3.1:25582966-25582985 | None:intergenic | 25.0% |
| ! | TTATGGATGCAAGTAATAAA+TGG | - | chr3.1:25585144-25585163 | MS.gene94013:intron | 25.0% |
| ! | TTCCAATGAATAACCTTATT+TGG | - | chr3.1:25583212-25583231 | MS.gene94013:intron | 25.0% |
| !! | GTTTTGTACTTTAAGCTTTA+TGG | - | chr3.1:25583268-25583287 | MS.gene94013:intron | 25.0% |
| !!! | ACTCTCTTTTTTACAATGTT+TGG | - | chr3.1:25583008-25583027 | MS.gene94013:intron | 25.0% |
| !!! | CCAACTAAATTGTCATTTTT+GGG | - | chr3.1:25583473-25583492 | MS.gene94013:intron | 25.0% |
| !!! | GATTTGTTATAGAGAACAAT+AGG | - | chr3.1:25584030-25584049 | MS.gene94013:intron | 25.0% |
| !!! | TCCAACTAAATTGTCATTTT+TGG | - | chr3.1:25583472-25583491 | MS.gene94013:intron | 25.0% |
| AAATCATGTATACATGGATG+AGG | + | chr3.1:25583098-25583117 | None:intergenic | 30.0% | |
| AAGGAAAAGTCATCACTTTA+AGG | - | chr3.1:25583724-25583743 | MS.gene94013:intron | 30.0% | |
| ACAAACACTACTTCAAAACA+TGG | + | chr3.1:25584171-25584190 | None:intergenic | 30.0% | |
| ACTAGTGACAACAATATCAT+TGG | + | chr3.1:25583942-25583961 | None:intergenic | 30.0% | |
| ACTGCATATTCTTAAATGGT+TGG | + | chr3.1:25585183-25585202 | None:intergenic | 30.0% | |
| AGAAACTATGTGAATGATGA+AGG | - | chr3.1:25583705-25583724 | MS.gene94013:intron | 30.0% | |
| AGGATTTGATCGATAAAGTT+TGG | - | chr3.1:25584205-25584224 | MS.gene94013:intron | 30.0% | |
| CAAACACTACTTCAAAACAT+GGG | + | chr3.1:25584170-25584189 | None:intergenic | 30.0% | |
| CCCAAAAATGACAATTTAGT+TGG | + | chr3.1:25583476-25583495 | None:intergenic | 30.0% | |
| CTAGTGACAACAATATCATT+GGG | + | chr3.1:25583941-25583960 | None:intergenic | 30.0% | |
| GACCAAATAAGGTTATTCAT+TGG | + | chr3.1:25583217-25583236 | None:intergenic | 30.0% | |
| GCTTAAAGTACAAAACAAAC+TGG | + | chr3.1:25583264-25583283 | None:intergenic | 30.0% | |
| TAACATTCATGTACTTAGGT+GGG | - | chr3.1:25584126-25584145 | MS.gene94013:intron | 30.0% | |
| TAGGTAACATTCATGTACTT+AGG | - | chr3.1:25584122-25584141 | MS.gene94013:intron | 30.0% | |
| TAGTGCAAAGATTGAAAAGT+AGG | + | chr3.1:25583831-25583850 | None:intergenic | 30.0% | |
| TATTCATTTATTGCTTGCCA+AGG | - | chr3.1:25582946-25582965 | MS.gene94013:intron | 30.0% | |
| TCCAATATATTAGTTGTCGA+AGG | + | chr3.1:25584349-25584368 | None:intergenic | 30.0% | |
| TCCTTCGACAACTAATATAT+TGG | - | chr3.1:25584345-25584364 | MS.gene94013:intron | 30.0% | |
| TGAATATATGGTTGAAACCA+TGG | + | chr3.1:25584463-25584482 | None:intergenic | 30.0% | |
| TGTCATCATATTACCTCAAA+TGG | + | chr3.1:25583761-25583780 | None:intergenic | 30.0% | |
| TTTAGAGATACATAGCAGAA+AGG | + | chr3.1:25583377-25583396 | None:intergenic | 30.0% | |
| ! | TTTTTCTGATTGTGATGTTC+AGG | - | chr3.1:25584261-25584280 | MS.gene94013:intron | 30.0% |
| !! | TTTGGTAGCATATTTTCTTC+TGG | - | chr3.1:25584567-25584586 | MS.gene94013:intron | 30.0% |
| !!! | AACAATAGGCTTTTTTGATC+AGG | - | chr3.1:25584044-25584063 | MS.gene94013:intron | 30.0% |
| !!! | ATTTTCTAGTTTCAGTGTGT+TGG | - | chr3.1:25583302-25583321 | MS.gene94013:intron | 30.0% |
| !!! | CATCGATTTTGTAACATTCA+TGG | + | chr3.1:25584827-25584846 | None:intergenic | 30.0% |
| !!! | GGAGTTTGGTTTTTTTGTTT+TGG | - | chr3.1:25584588-25584607 | MS.gene94013:intron | 30.0% |
| !!! | TTGTCATTTTTGGGTTCAAT+TGG | - | chr3.1:25583482-25583501 | MS.gene94013:intron | 30.0% |
| !!! | TTTTCTAGTTTCAGTGTGTT+GGG | - | chr3.1:25583303-25583322 | MS.gene94013:intron | 30.0% |
| !!! | TTTTTTGATCAGGATGGTAA+CGG | - | chr3.1:25584054-25584073 | MS.gene94013:intron | 30.0% |
| AAACACACTCAGATAGAGAA+GGG | + | chr3.1:25582927-25582946 | None:intergenic | 35.0% | |
| AGTTTATTGCAGTGTATGGA+AGG | - | chr3.1:25584236-25584255 | MS.gene94013:intron | 35.0% | |
| CATACATCTAGAAACAGATC+AGG | - | chr3.1:25584375-25584394 | MS.gene94013:intron | 35.0% | |
| CTAAGTTACTTGATATTGGC+AGG | - | chr3.1:25585097-25585116 | MS.gene94013:intron | 35.0% | |
| GGTTATTCATTGGAATCAAC+AGG | + | chr3.1:25583207-25583226 | None:intergenic | 35.0% | |
| GTAACATTCATGTACTTAGG+TGG | - | chr3.1:25584125-25584144 | MS.gene94013:intron | 35.0% | |
| GTCAACTTGTGTAAGAGAAA+AGG | + | chr3.1:25584441-25584460 | None:intergenic | 35.0% | |
| GTCAAGTTTATTGCAGTGTA+TGG | - | chr3.1:25584232-25584251 | MS.gene94013:intron | 35.0% | |
| GTTTGTTTGTCTACTTGTGT+AGG | - | chr3.1:25584185-25584204 | MS.gene94013:intron | 35.0% | |
| TAAACACACTCAGATAGAGA+AGG | + | chr3.1:25582928-25582947 | None:intergenic | 35.0% | |
| TGTTTGTGCAAGATATGCAT+CGG | + | chr3.1:25584993-25585012 | None:intergenic | 35.0% | |
| ! | GAAGTTGATCAATGCTAAAG+AGG | - | chr3.1:25583998-25584017 | MS.gene94013:intron | 35.0% |
| ! | TCATCACTTTAAGGAAAAGC+CGG | - | chr3.1:25583733-25583752 | MS.gene94013:intron | 35.0% |
| ! | TTCAAGATGCGTTTTCCTTT+GGG | - | chr3.1:25583398-25583417 | MS.gene94013:intron | 35.0% |
| ! | TTGTGATGTTCAGGTTTAGT+TGG | - | chr3.1:25584270-25584289 | MS.gene94013:intron | 35.0% |
| ! | TTTAGTTGGTGTGTCATCTT+GGG | - | chr3.1:25584284-25584303 | MS.gene94013:intron | 35.0% |
| !! | CTGATCTGTTTCTAGATGTA+TGG | + | chr3.1:25584377-25584396 | None:intergenic | 35.0% |
| !! | GTTCAATTGGTGTCATTCAA+GGG | - | chr3.1:25583495-25583514 | MS.gene94013:intron | 35.0% |
| !! | TCTGTTTCTAGATGTATGGA+AGG | + | chr3.1:25584373-25584392 | None:intergenic | 35.0% |
| !!! | AGTTTTGGAGCTTTGTTTTG+CGG | - | chr3.1:25583542-25583561 | MS.gene94013:intron | 35.0% |
| !!! | ATAGGCTTTTTTGATCAGGA+TGG | - | chr3.1:25584048-25584067 | MS.gene94013:intron | 35.0% |
| !!! | GCATATTTTCTTCTGGAGTT+TGG | - | chr3.1:25584574-25584593 | MS.gene94013:intron | 35.0% |
| AAGACATGCATGTAATGGTG+AGG | - | chr3.1:25583659-25583678 | MS.gene94013:intron | 40.0% | |
| AGTTACTTGATATTGGCAGG+TGG | - | chr3.1:25585100-25585119 | MS.gene94013:intron | 40.0% | |
| CATATTACCTCAAATGGTGC+CGG | + | chr3.1:25583755-25583774 | None:intergenic | 40.0% | |
| GGAAAATGCATCAAACCCAA+AGG | + | chr3.1:25583416-25583435 | None:intergenic | 40.0% | |
| TCATCTTGGGGATATTGTTG+CGG | - | chr3.1:25584297-25584316 | MS.gene94013:intron | 40.0% | |
| TCTTCTTCCTTGCTTCTTTG+TGG | - | chr3.1:25584534-25584553 | MS.gene94013:intron | 40.0% | |
| TTCAGTGTGTTGGGATTTGT+TGG | - | chr3.1:25583312-25583331 | MS.gene94013:intron | 40.0% | |
| ! | GTTCAAGATGCGTTTTCCTT+TGG | - | chr3.1:25583397-25583416 | MS.gene94013:intron | 40.0% |
| ! | GTTTAGTTGGTGTGTCATCT+TGG | - | chr3.1:25584283-25584302 | MS.gene94013:intron | 40.0% |
| ! | TCTCTTACACAAGTTGACCA+TGG | - | chr3.1:25584443-25584462 | MS.gene94013:intron | 40.0% |
| ! | TGGTTTTCAACCCCTATGAA+GGG | - | chr3.1:25584608-25584627 | MS.gene94013:intron | 40.0% |
| ! | TGTTTTGCGGCTGTTTGAAT+AGG | - | chr3.1:25583555-25583574 | MS.gene94013:intron | 40.0% |
| ! | TTAGTTGGTGTGTCATCTTG+GGG | - | chr3.1:25584285-25584304 | MS.gene94013:intron | 40.0% |
| ! | TTGGGTTTGATGCATTTTCC+CGG | - | chr3.1:25583416-25583435 | MS.gene94013:intron | 40.0% |
| !! | GGTTCAATTGGTGTCATTCA+AGG | - | chr3.1:25583494-25583513 | MS.gene94013:intron | 40.0% |
| !! | TTGGTTTTCAACCCCTATGA+AGG | - | chr3.1:25584607-25584626 | MS.gene94013:intron | 40.0% |
| !!! | CTTTGTGGCTCCTGTTTTTT+TGG | - | chr3.1:25584549-25584568 | MS.gene94013:intron | 40.0% |
| AGCAGGAAAACACCCTTCAT+AGG | + | chr3.1:25584623-25584642 | None:intergenic | 45.0% | |
| CAGATCTGAACAACACAACC+GGG | + | chr3.1:25583437-25583456 | None:intergenic | 45.0% | |
| CAGGAAAACACCCTTCATAG+GGG | + | chr3.1:25584621-25584640 | None:intergenic | 45.0% | |
| GCAGATCTGAACAACACAAC+CGG | + | chr3.1:25583438-25583457 | None:intergenic | 45.0% | |
| GCAGGAAAACACCCTTCATA+GGG | + | chr3.1:25584622-25584641 | None:intergenic | 45.0% | |
| TTGCTATCTGTCAAGGCTGA+AGG | - | chr3.1:25583519-25583538 | MS.gene94013:intron | 45.0% | |
| ! | TGTCAAGGCTGAAGGAGTTT+TGG | - | chr3.1:25583527-25583546 | MS.gene94013:intron | 45.0% |
| !! | CAAGGGTTTGCTATCTGTCA+AGG | - | chr3.1:25583512-25583531 | MS.gene94013:intron | 45.0% |
| !! | TTGTTGGCTTATGTCGCTTG+TGG | - | chr3.1:25583328-25583347 | MS.gene94013:intron | 45.0% |
| !!! | TATGAAGGGTGTTTTCCTGC+TGG | - | chr3.1:25584622-25584641 | MS.gene94013:intron | 45.0% |
| !!! | AATCTAATTTTAATTATAAT+TGG | - | chr3.1:25582980-25582999 | MS.gene94013:intron | 5.0% |
| ACAGGAGCCACAAAGAAGCA+AGG | + | chr3.1:25584544-25584563 | None:intergenic | 50.0% | |
| ATACAGACTGCTACACCAGC+AGG | + | chr3.1:25584640-25584659 | None:intergenic | 50.0% | |
| GTACTTAGGTGGGTGTGAGA+TGG | - | chr3.1:25584136-25584155 | MS.gene94013:intron | 50.0% | |
| GGAAAAGCCGGCACCATTTG+AGG | - | chr3.1:25583745-25583764 | MS.gene94013:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 25582908 | 25585347 | 25582908 | ID=MS.gene94013 |
| chr3.1 | mRNA | 25582908 | 25585347 | 25582908 | ID=MS.gene94013.t1;Parent=MS.gene94013 |
| chr3.1 | exon | 25585288 | 25585347 | 25585288 | ID=MS.gene94013.t1.exon1;Parent=MS.gene94013.t1 |
| chr3.1 | CDS | 25585288 | 25585347 | 25585288 | ID=cds.MS.gene94013.t1;Parent=MS.gene94013.t1 |
| chr3.1 | exon | 25583859 | 25583966 | 25583859 | ID=MS.gene94013.t1.exon2;Parent=MS.gene94013.t1 |
| chr3.1 | CDS | 25583859 | 25583966 | 25583859 | ID=cds.MS.gene94013.t1;Parent=MS.gene94013.t1 |
| chr3.1 | exon | 25583100 | 25583136 | 25583100 | ID=MS.gene94013.t1.exon3;Parent=MS.gene94013.t1 |
| chr3.1 | CDS | 25583100 | 25583136 | 25583100 | ID=cds.MS.gene94013.t1;Parent=MS.gene94013.t1 |
| chr3.1 | exon | 25583016 | 25583053 | 25583016 | ID=MS.gene94013.t1.exon4;Parent=MS.gene94013.t1 |
| chr3.1 | CDS | 25583016 | 25583053 | 25583016 | ID=cds.MS.gene94013.t1;Parent=MS.gene94013.t1 |
| chr3.1 | exon | 25582908 | 25582913 | 25582908 | ID=MS.gene94013.t1.exon5;Parent=MS.gene94013.t1 |
| chr3.1 | CDS | 25582908 | 25582913 | 25582908 | ID=cds.MS.gene94013.t1;Parent=MS.gene94013.t1 |
| Gene Sequence |
| Protein sequence |
