Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94210.t1 | XP_003616694.1 | 95.1 | 205 | 10 | 0 | 1 | 205 | 1 | 205 | 1.10E-106 | 396 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94210.t1 | O80920 | 72.7 | 165 | 44 | 1 | 39 | 203 | 32 | 195 | 1.6e-61 | 237.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94210.t1 | G7K772 | 95.1 | 205 | 10 | 0 | 1 | 205 | 1 | 205 | 7.8e-107 | 396.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052795 | MS.gene94210 | 0.848028 | 7.95E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94210.t1 | MTR_5g083270 | 95.122 | 205 | 10 | 0 | 1 | 205 | 1 | 205 | 1.68e-145 | 403 |
| MS.gene94210.t1 | MTR_3g071740 | 62.500 | 224 | 63 | 5 | 1 | 204 | 1 | 223 | 2.29e-83 | 247 |
| MS.gene94210.t1 | MTR_4g014460 | 64.327 | 171 | 54 | 4 | 39 | 205 | 42 | 209 | 1.29e-71 | 216 |
| MS.gene94210.t1 | MTR_7g070050 | 68.000 | 175 | 46 | 4 | 39 | 205 | 6 | 178 | 2.18e-70 | 212 |
| MS.gene94210.t1 | MTR_5g030500 | 55.488 | 164 | 66 | 3 | 46 | 203 | 44 | 206 | 2.11e-60 | 188 |
| MS.gene94210.t1 | MTR_8g027805 | 52.353 | 170 | 73 | 4 | 41 | 205 | 11 | 177 | 8.06e-58 | 181 |
| MS.gene94210.t1 | MTR_3g090980 | 51.534 | 163 | 73 | 3 | 44 | 203 | 19 | 178 | 2.91e-57 | 179 |
| MS.gene94210.t1 | MTR_1g028380 | 50.909 | 165 | 75 | 2 | 44 | 205 | 17 | 178 | 1.06e-56 | 178 |
| MS.gene94210.t1 | MTR_1g016480 | 45.226 | 199 | 94 | 4 | 16 | 202 | 1 | 196 | 1.17e-54 | 173 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94210.t1 | AT2G40330 | 58.095 | 210 | 80 | 3 | 1 | 203 | 1 | 209 | 1.11e-80 | 239 |
| MS.gene94210.t1 | AT5G05440 | 60.396 | 202 | 74 | 3 | 1 | 200 | 1 | 198 | 1.82e-76 | 228 |
| MS.gene94210.t1 | AT2G38310 | 65.285 | 193 | 60 | 3 | 13 | 203 | 8 | 195 | 3.17e-73 | 220 |
| MS.gene94210.t1 | AT1G01360 | 52.147 | 163 | 72 | 2 | 46 | 205 | 21 | 180 | 2.16e-60 | 187 |
| MS.gene94210.t1 | AT4G27920 | 50.909 | 165 | 76 | 2 | 44 | 205 | 12 | 174 | 1.32e-57 | 180 |
| MS.gene94210.t1 | AT4G01026 | 52.201 | 159 | 70 | 3 | 50 | 205 | 27 | 182 | 3.57e-56 | 177 |
| MS.gene94210.t1 | AT5G53160 | 50.909 | 165 | 75 | 3 | 44 | 205 | 17 | 178 | 4.37e-56 | 176 |
| MS.gene94210.t1 | AT4G17870 | 50.610 | 164 | 77 | 2 | 45 | 205 | 16 | 178 | 4.01e-53 | 169 |
| MS.gene94210.t1 | AT2G26040 | 49.383 | 162 | 75 | 3 | 46 | 202 | 22 | 181 | 1.61e-50 | 162 |
| MS.gene94210.t1 | AT1G73000 | 47.619 | 168 | 77 | 3 | 46 | 202 | 37 | 204 | 1.03e-49 | 160 |
| MS.gene94210.t1 | AT5G46790 | 51.765 | 170 | 75 | 2 | 42 | 205 | 40 | 208 | 8.49e-49 | 159 |
| MS.gene94210.t1 | AT5G46790 | 51.765 | 170 | 75 | 2 | 42 | 205 | 40 | 208 | 8.49e-49 | 159 |
| MS.gene94210.t1 | AT5G45860 | 58.389 | 149 | 58 | 3 | 59 | 205 | 10 | 156 | 1.28e-46 | 151 |
| MS.gene94210.t1 | AT5G45870 | 56.954 | 151 | 62 | 3 | 56 | 205 | 7 | 155 | 2.42e-45 | 148 |
| MS.gene94210.t1 | AT4G18620 | 46.914 | 162 | 79 | 2 | 51 | 205 | 1 | 162 | 1.33e-35 | 123 |
| MS.gene94210.t1 | AT5G53160 | 46.667 | 105 | 53 | 2 | 44 | 148 | 17 | 118 | 6.60e-28 | 103 |
Find 59 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTGGTTTGCAACCGTTTC+TGG | 0.080678 | 5.4:+66036761 | None:intergenic |
| CGTGAAGTTCGACTTGTTTC+CGG | 0.131980 | 5.4:-66036563 | MS.gene94210:CDS |
| AAACGGAGGCCTTGATGGTT+TGG | 0.349101 | 5.4:+66036696 | None:intergenic |
| GTGTTGTTGGTGGTGTTCAT+AGG | 0.363891 | 5.4:-66036472 | MS.gene94210:CDS |
| TCTAACACCGCCATGACTAA+TGG | 0.405607 | 5.4:-66036854 | MS.gene94210:CDS |
| GTTATTAGTTTCAGTGTTGT+TGG | 0.408321 | 5.4:-66036485 | MS.gene94210:CDS |
| TTCATAGGTGTAGGAATTAC+CGG | 0.411086 | 5.4:-66036457 | MS.gene94210:CDS |
| AGGCCTCCGTTTCAACTGTC+TGG | 0.430513 | 5.4:-66036685 | MS.gene94210:CDS |
| CGACACGCTTGAATTTGTTT+CGG | 0.430906 | 5.4:+66036821 | None:intergenic |
| AGTTGAAACGGAGGCCTTGA+TGG | 0.442287 | 5.4:+66036691 | None:intergenic |
| TCGGTGTAATTTGCAGTCAC+TGG | 0.459352 | 5.4:-66036315 | MS.gene94210:CDS |
| GGTGGTTTGCAACCGTTTCT+GGG | 0.462922 | 5.4:+66036762 | None:intergenic |
| AACGTAGTTGCCTCCGGTGA+TGG | 0.485651 | 5.4:-66036605 | MS.gene94210:CDS |
| AACCGTTTCTGTCCCAGAAA+CGG | 0.488981 | 5.4:-66036774 | MS.gene94210:CDS |
| AGCTGCAACGTAGTTGCCTC+CGG | 0.500297 | 5.4:-66036611 | MS.gene94210:CDS |
| GCTTGAAACGGCAGGTAGAC+CGG | 0.502003 | 5.4:+66036544 | None:intergenic |
| CAACCTCTCCATGCTTGAAA+CGG | 0.516613 | 5.4:+66036532 | None:intergenic |
| GGATCACGACGGAGTAGCAC+TGG | 0.520709 | 5.4:+66036717 | None:intergenic |
| AACCGTTTCTGGGACAGAAA+CGG | 0.521454 | 5.4:+66036772 | None:intergenic |
| CGTTTCAAGCATGGAGAGGT+TGG | 0.529991 | 5.4:-66036531 | MS.gene94210:CDS |
| TCGAACCGTTGAAATTGAAC+CGG | 0.530260 | 5.4:+66036878 | None:intergenic |
| CGTTGAAATTGAACCGGTGA+AGG | 0.532412 | 5.4:+66036884 | None:intergenic |
| ACCGACACGAATGCCATCAC+CGG | 0.534492 | 5.4:+66036592 | None:intergenic |
| AACGGACCAGACAGTTGAAA+CGG | 0.535986 | 5.4:+66036679 | None:intergenic |
| CAAAATAGAAATGCCTTCAC+CGG | 0.537348 | 5.4:-66036897 | None:intergenic |
| CTTCACCGGTTCAATTTCAA+CGG | 0.537786 | 5.4:-66036883 | MS.gene94210:CDS |
| CTCTCCATGCTTGAAACGGC+AGG | 0.544665 | 5.4:+66036536 | None:intergenic |
| ACAAAGTGTTTGTAGCCTTG+TGG | 0.546590 | 5.4:+66036638 | None:intergenic |
| TCTACCTGCCGTTTCAAGCA+TGG | 0.550915 | 5.4:-66036540 | MS.gene94210:CDS |
| CGGTCAGTGACGACGTTGCA+CGG | 0.551799 | 5.4:-66036437 | MS.gene94210:CDS |
| TTGGTGGTGTTCATAGGTGT+AGG | 0.559793 | 5.4:-66036466 | MS.gene94210:CDS |
| ATTAGTTTCAGTGTTGTTGG+TGG | 0.561597 | 5.4:-66036482 | MS.gene94210:CDS |
| CGTCGTGATCCAAACCATCA+AGG | 0.571418 | 5.4:-66036705 | MS.gene94210:CDS |
| ATACGTCGTTGATGTACCAC+AGG | 0.575028 | 5.4:-66036381 | MS.gene94210:CDS |
| CGTGCAACGTCGTCACTGAC+CGG | 0.576112 | 5.4:+66036438 | None:intergenic |
| ACAGTCAATGCCATTAGTCA+TGG | 0.577189 | 5.4:+66036844 | None:intergenic |
| CAACACTGAAACTAATAACA+TGG | 0.585088 | 5.4:+66036489 | None:intergenic |
| ACGTTGCACGGTGACGGTAA+CGG | 0.591404 | 5.4:-66036425 | MS.gene94210:CDS |
| GTGACGACGTTGCACGGTGA+CGG | 0.594931 | 5.4:-66036431 | MS.gene94210:CDS |
| AGCCTTGTGGATTGTCGAAG+CGG | 0.601019 | 5.4:+66036651 | None:intergenic |
| AACCACCACATGCACGTGGT+GGG | 0.602296 | 5.4:-66036746 | MS.gene94210:CDS |
| TCCGGTGATGGCATTCGTGT+CGG | 0.606951 | 5.4:-66036593 | MS.gene94210:CDS |
| TGTACCACAGGGTAACACTA+AGG | 0.609377 | 5.4:-66036369 | MS.gene94210:CDS |
| CGCCGCTTCGACAATCCACA+AGG | 0.611657 | 5.4:-66036653 | MS.gene94210:CDS |
| AAACCACCACATGCACGTGG+TGG | 0.622421 | 5.4:-66036747 | MS.gene94210:CDS |
| GTCCCACCACGTGCATGTGG+TGG | 0.623392 | 5.4:+66036744 | None:intergenic |
| CTGCCGTTTCAAGCATGGAG+AGG | 0.630256 | 5.4:-66036535 | MS.gene94210:CDS |
| ATTGTCGAAGCGGCGGACAA+CGG | 0.630831 | 5.4:+66036661 | None:intergenic |
| CTGCAAATTACACCGAACAA+TGG | 0.632705 | 5.4:+66036322 | None:intergenic |
| TGCAAACCACCACATGCACG+TGG | 0.642151 | 5.4:-66036750 | MS.gene94210:CDS |
| TTTGTCCCACCACGTGCATG+TGG | 0.645948 | 5.4:+66036741 | None:intergenic |
| TCTTCCTTAGTGTTACCCTG+TGG | 0.656284 | 5.4:+66036365 | None:intergenic |
| GACACGAATGCCATCACCGG+AGG | 0.657865 | 5.4:+66036595 | None:intergenic |
| GTCAATGCCATTAGTCATGG+CGG | 0.679359 | 5.4:+66036847 | None:intergenic |
| CTTGTGGATTGTCGAAGCGG+CGG | 0.689521 | 5.4:+66036654 | None:intergenic |
| CTTGATGGTTTGGATCACGA+CGG | 0.697791 | 5.4:+66036706 | None:intergenic |
| GGACCAGACAGTTGAAACGG+AGG | 0.712986 | 5.4:+66036682 | None:intergenic |
| TTGCACGGTGACGGTAACGG+AGG | 0.729282 | 5.4:-66036422 | MS.gene94210:CDS |
| TACGTCGTTGATGTACCACA+GGG | 0.754269 | 5.4:-66036380 | MS.gene94210:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAACACTGAAACTAATAACA+TGG | + | chr5.4:66036693-66036712 | None:intergenic | 30.0% | |
| ! | GTTATTAGTTTCAGTGTTGT+TGG | - | chr5.4:66036694-66036713 | MS.gene94210:CDS | 30.0% |
| !! | GTTTTGTTGATACCATTGTT+CGG | - | chr5.4:66036845-66036864 | MS.gene94210:CDS | 30.0% |
| TTCATAGGTGTAGGAATTAC+CGG | - | chr5.4:66036722-66036741 | MS.gene94210:CDS | 35.0% | |
| ! | ATTAGTTTCAGTGTTGTTGG+TGG | - | chr5.4:66036697-66036716 | MS.gene94210:CDS | 35.0% |
| ACAGTCAATGCCATTAGTCA+TGG | + | chr5.4:66036338-66036357 | None:intergenic | 40.0% | |
| CTGCAAATTACACCGAACAA+TGG | + | chr5.4:66036860-66036879 | None:intergenic | 40.0% | |
| TCGAACCGTTGAAATTGAAC+CGG | + | chr5.4:66036304-66036323 | None:intergenic | 40.0% | |
| ! | ACAAAGTGTTTGTAGCCTTG+TGG | + | chr5.4:66036544-66036563 | None:intergenic | 40.0% |
| ! | CGACACGCTTGAATTTGTTT+CGG | + | chr5.4:66036361-66036380 | None:intergenic | 40.0% |
| !! | CTTCACCGGTTCAATTTCAA+CGG | - | chr5.4:66036296-66036315 | MS.gene94210:CDS | 40.0% |
| AACCGTTTCTGTCCCAGAAA+CGG | - | chr5.4:66036405-66036424 | MS.gene94210:CDS | 45.0% | |
| AACGGACCAGACAGTTGAAA+CGG | + | chr5.4:66036503-66036522 | None:intergenic | 45.0% | |
| ATACGTCGTTGATGTACCAC+AGG | - | chr5.4:66036798-66036817 | MS.gene94210:CDS | 45.0% | |
| CAACCTCTCCATGCTTGAAA+CGG | + | chr5.4:66036650-66036669 | None:intergenic | 45.0% | |
| CGTTGAAATTGAACCGGTGA+AGG | + | chr5.4:66036298-66036317 | None:intergenic | 45.0% | |
| GTCAATGCCATTAGTCATGG+CGG | + | chr5.4:66036335-66036354 | None:intergenic | 45.0% | |
| TACGTCGTTGATGTACCACA+GGG | - | chr5.4:66036799-66036818 | MS.gene94210:CDS | 45.0% | |
| TCTAACACCGCCATGACTAA+TGG | - | chr5.4:66036325-66036344 | MS.gene94210:CDS | 45.0% | |
| TCTTCCTTAGTGTTACCCTG+TGG | + | chr5.4:66036817-66036836 | None:intergenic | 45.0% | |
| TGTACCACAGGGTAACACTA+AGG | - | chr5.4:66036810-66036829 | MS.gene94210:CDS | 45.0% | |
| ! | CGTGAAGTTCGACTTGTTTC+CGG | - | chr5.4:66036616-66036635 | MS.gene94210:CDS | 45.0% |
| ! | CTTGATGGTTTGGATCACGA+CGG | + | chr5.4:66036476-66036495 | None:intergenic | 45.0% |
| ! | TCGGTGTAATTTGCAGTCAC+TGG | - | chr5.4:66036864-66036883 | MS.gene94210:CDS | 45.0% |
| !! | AACCGTTTCTGGGACAGAAA+CGG | + | chr5.4:66036410-66036429 | None:intergenic | 45.0% |
| !! | GTGTTGTTGGTGGTGTTCAT+AGG | - | chr5.4:66036707-66036726 | MS.gene94210:CDS | 45.0% |
| !! | TTGGTGGTGTTCATAGGTGT+AGG | - | chr5.4:66036713-66036732 | MS.gene94210:CDS | 45.0% |
| AGTTGAAACGGAGGCCTTGA+TGG | + | chr5.4:66036491-66036510 | None:intergenic | 50.0% | |
| CGTCGTGATCCAAACCATCA+AGG | - | chr5.4:66036474-66036493 | MS.gene94210:CDS | 50.0% | |
| CGTTTCAAGCATGGAGAGGT+TGG | - | chr5.4:66036648-66036667 | MS.gene94210:CDS | 50.0% | |
| ! | AAACGGAGGCCTTGATGGTT+TGG | + | chr5.4:66036486-66036505 | None:intergenic | 50.0% |
| ! | AGCCTTGTGGATTGTCGAAG+CGG | + | chr5.4:66036531-66036550 | None:intergenic | 50.0% |
| ! | GGTGGTTTGCAACCGTTTCT+GGG | + | chr5.4:66036420-66036439 | None:intergenic | 50.0% |
| ! | TCTACCTGCCGTTTCAAGCA+TGG | - | chr5.4:66036639-66036658 | MS.gene94210:CDS | 50.0% |
| ! | TGGTGGTTTGCAACCGTTTC+TGG | + | chr5.4:66036421-66036440 | None:intergenic | 50.0% |
| AAACCACCACATGCACGTGG+TGG | - | chr5.4:66036432-66036451 | MS.gene94210:CDS | 55.0% | |
| AACCACCACATGCACGTGGT+GGG | - | chr5.4:66036433-66036452 | MS.gene94210:CDS | 55.0% | |
| AACGTAGTTGCCTCCGGTGA+TGG | - | chr5.4:66036574-66036593 | MS.gene94210:CDS | 55.0% | |
| ACCGACACGAATGCCATCAC+CGG | + | chr5.4:66036590-66036609 | None:intergenic | 55.0% | |
| AGCTGCAACGTAGTTGCCTC+CGG | - | chr5.4:66036568-66036587 | MS.gene94210:CDS | 55.0% | |
| AGGCCTCCGTTTCAACTGTC+TGG | - | chr5.4:66036494-66036513 | MS.gene94210:CDS | 55.0% | |
| ATTGTCGAAGCGGCGGACAA+CGG | + | chr5.4:66036521-66036540 | None:intergenic | 55.0% | |
| CTCTCCATGCTTGAAACGGC+AGG | + | chr5.4:66036646-66036665 | None:intergenic | 55.0% | |
| GCTTGAAACGGCAGGTAGAC+CGG | + | chr5.4:66036638-66036657 | None:intergenic | 55.0% | |
| GGACCAGACAGTTGAAACGG+AGG | + | chr5.4:66036500-66036519 | None:intergenic | 55.0% | |
| TGCAAACCACCACATGCACG+TGG | - | chr5.4:66036429-66036448 | MS.gene94210:CDS | 55.0% | |
| TTTGTCCCACCACGTGCATG+TGG | + | chr5.4:66036441-66036460 | None:intergenic | 55.0% | |
| ! | ACGTTGCACGGTGACGGTAA+CGG | - | chr5.4:66036754-66036773 | MS.gene94210:CDS | 55.0% |
| ! | CTGCCGTTTCAAGCATGGAG+AGG | - | chr5.4:66036644-66036663 | MS.gene94210:CDS | 55.0% |
| ! | CTTGTGGATTGTCGAAGCGG+CGG | + | chr5.4:66036528-66036547 | None:intergenic | 55.0% |
| ! | TCCGGTGATGGCATTCGTGT+CGG | - | chr5.4:66036586-66036605 | MS.gene94210:CDS | 55.0% |
| CGCCGCTTCGACAATCCACA+AGG | - | chr5.4:66036526-66036545 | MS.gene94210:CDS | 60.0% | |
| CGGTCAGTGACGACGTTGCA+CGG | - | chr5.4:66036742-66036761 | MS.gene94210:CDS | 60.0% | |
| CGTGCAACGTCGTCACTGAC+CGG | + | chr5.4:66036744-66036763 | None:intergenic | 60.0% | |
| GACACGAATGCCATCACCGG+AGG | + | chr5.4:66036587-66036606 | None:intergenic | 60.0% | |
| GGATCACGACGGAGTAGCAC+TGG | + | chr5.4:66036465-66036484 | None:intergenic | 60.0% | |
| GTGACGACGTTGCACGGTGA+CGG | - | chr5.4:66036748-66036767 | MS.gene94210:CDS | 60.0% | |
| ! | TTGCACGGTGACGGTAACGG+AGG | - | chr5.4:66036757-66036776 | MS.gene94210:CDS | 60.0% |
| GTCCCACCACGTGCATGTGG+TGG | + | chr5.4:66036438-66036457 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 66036292 | 66036909 | 66036292 | ID=MS.gene94210 |
| chr5.4 | mRNA | 66036292 | 66036909 | 66036292 | ID=MS.gene94210.t1;Parent=MS.gene94210 |
| chr5.4 | exon | 66036292 | 66036909 | 66036292 | ID=MS.gene94210.t1.exon1;Parent=MS.gene94210.t1 |
| chr5.4 | CDS | 66036292 | 66036909 | 66036292 | ID=cds.MS.gene94210.t1;Parent=MS.gene94210.t1 |
| Gene Sequence |
| Protein sequence |
