Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94303.t1 | XP_013452335.1 | 87.4 | 277 | 31 | 1 | 252 | 528 | 82 | 354 | 1.40E-126 | 463.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94303.t1 | Q8LG95 | 72.2 | 209 | 58 | 0 | 252 | 460 | 35 | 243 | 6.1e-86 | 319.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94303.t1 | A0A072UBE9 | 87.4 | 277 | 31 | 1 | 252 | 528 | 82 | 354 | 1.0e-126 | 463.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene94303 | 0.810634 | 1.05E-50 | -1.69E-46 |
| MS.gene060084 | MS.gene94303 | 0.8074 | 5.15E-50 | -1.69E-46 |
| MS.gene060476 | MS.gene94303 | 0.800568 | 1.36E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94303.t1 | MTR_6g053090 | 95.390 | 282 | 9 | 1 | 247 | 528 | 77 | 354 | 0.0 | 546 |
| MS.gene94303.t1 | MTR_3g024400 | 46.500 | 200 | 106 | 1 | 262 | 461 | 76 | 274 | 5.39e-53 | 181 |
| MS.gene94303.t1 | MTR_1g050480 | 92.000 | 75 | 6 | 0 | 68 | 142 | 253 | 327 | 3.67e-41 | 151 |
| MS.gene94303.t1 | MTR_5g012120 | 39.744 | 156 | 93 | 1 | 286 | 440 | 4 | 159 | 5.62e-34 | 127 |
| MS.gene94303.t1 | MTR_5g012120 | 39.744 | 156 | 93 | 1 | 286 | 440 | 81 | 236 | 2.39e-33 | 128 |
| MS.gene94303.t1 | MTR_3g024400 | 45.455 | 132 | 71 | 1 | 330 | 461 | 96 | 226 | 9.76e-31 | 119 |
| MS.gene94303.t1 | MTR_4g085790 | 34.731 | 167 | 109 | 0 | 1 | 167 | 1 | 167 | 1.72e-28 | 113 |
| MS.gene94303.t1 | MTR_7g025150 | 35.625 | 160 | 103 | 0 | 9 | 168 | 9 | 168 | 1.86e-28 | 113 |
| MS.gene94303.t1 | MTR_1g054485 | 32.759 | 174 | 116 | 1 | 1 | 174 | 1 | 173 | 2.69e-24 | 100 |
| MS.gene94303.t1 | MTR_7g039210 | 36.029 | 136 | 85 | 2 | 5 | 139 | 6 | 140 | 2.51e-23 | 99.4 |
| MS.gene94303.t1 | MTR_1g054495 | 32.026 | 153 | 103 | 1 | 12 | 164 | 12 | 163 | 1.34e-22 | 97.1 |
| MS.gene94303.t1 | MTR_0014s0220 | 34.058 | 138 | 91 | 0 | 2 | 139 | 620 | 757 | 1.90e-21 | 99.0 |
| MS.gene94303.t1 | MTR_4g069750 | 36.782 | 87 | 55 | 0 | 9 | 95 | 9 | 95 | 4.05e-15 | 71.6 |
| MS.gene94303.t1 | MTR_4g123470 | 25.243 | 206 | 123 | 1 | 14 | 188 | 17 | 222 | 1.01e-13 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94303.t1 | AT4G21190 | 71.963 | 214 | 60 | 0 | 247 | 460 | 30 | 243 | 1.98e-111 | 333 |
| MS.gene94303.t1 | AT4G18975 | 47.208 | 197 | 103 | 1 | 262 | 458 | 58 | 253 | 2.40e-56 | 189 |
| MS.gene94303.t1 | AT4G18975 | 47.208 | 197 | 103 | 1 | 262 | 458 | 85 | 280 | 4.71e-56 | 189 |
| MS.gene94303.t1 | AT4G18975 | 47.208 | 197 | 103 | 1 | 262 | 458 | 85 | 280 | 4.71e-56 | 189 |
| MS.gene94303.t1 | AT4G18975 | 47.208 | 197 | 103 | 1 | 262 | 458 | 85 | 280 | 4.71e-56 | 189 |
| MS.gene94303.t1 | AT1G04590 | 33.594 | 256 | 154 | 4 | 260 | 504 | 131 | 381 | 6.67e-35 | 135 |
| MS.gene94303.t1 | AT1G04590 | 33.591 | 259 | 153 | 5 | 260 | 504 | 131 | 384 | 1.69e-33 | 131 |
Find 112 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGTTCGTTTGTCATTTC+AGG | 0.166797 | 6.2:+42967185 | MS.gene94303:CDS |
| ACGTATCTATCAACTTTATT+AGG | 0.201297 | 6.2:-42975283 | None:intergenic |
| TTGGTTGAGTTTCGTTATTA+TGG | 0.227889 | 6.2:+42967501 | MS.gene94303:CDS |
| TGATTTCCTTCTGCATATTA+TGG | 0.235368 | 6.2:+42969308 | MS.gene94303:CDS |
| TCCTATAATTACAGTGAAAA+AGG | 0.241597 | 6.2:+42973209 | MS.gene94303:CDS |
| TTTGATCGATGGAGGGTATT+AGG | 0.256472 | 6.2:+42967127 | None:intergenic |
| AGGTAATCAGCGAAAGTTTC+AGG | 0.264590 | 6.2:-42967777 | None:intergenic |
| GACAATGTTCAACGAAATTC+AGG | 0.282692 | 6.2:+42975316 | MS.gene94303:CDS |
| AATGTCAAAGAAGAAGTTTA+TGG | 0.291907 | 6.2:+42973150 | MS.gene94303:CDS |
| TGGTTGAGTTTCGTTATTAT+GGG | 0.293148 | 6.2:+42967502 | MS.gene94303:CDS |
| AGATGAGTCATCTGAGTTTA+CGG | 0.299452 | 6.2:+42975441 | MS.gene94303:CDS |
| GGATGTTGCGAAGTCAAAAT+TGG | 0.300090 | 6.2:+42967647 | MS.gene94303:CDS |
| TGGATTTCCTGAGGATGTTT+TGG | 0.304500 | 6.2:+42967482 | MS.gene94303:CDS |
| GTGGATCTCCACTTCTACTA+TGG | 0.307411 | 6.2:-42967587 | None:intergenic |
| GAACAGGATGCCGATTTAAT+AGG | 0.309673 | 6.2:+42975397 | MS.gene94303:CDS |
| GGAGATCCACAAGAAACTTT+TGG | 0.337222 | 6.2:+42967600 | MS.gene94303:CDS |
| GATGTATTTATAGGAGGTAT+TGG | 0.339617 | 6.2:+42968966 | MS.gene94303:intron |
| AAGGAAAGCGTGTAAGAATC+AGG | 0.347993 | 6.2:+42975248 | MS.gene94303:CDS |
| TGAAACTATAGGTAACAAAA+TGG | 0.351629 | 6.2:+42973458 | MS.gene94303:intron |
| GACCAAGATATCCTCGAGTT+TGG | 0.361562 | 6.2:+42971746 | MS.gene94303:CDS |
| GCTTGGTAGTGTTATTGTCT+TGG | 0.365307 | 6.2:+42969018 | MS.gene94303:CDS |
| CGATATGTTTAACGATCGTT+TGG | 0.365893 | 6.2:+42967347 | MS.gene94303:CDS |
| TGACTCATCTGAAATTTGTT+TGG | 0.365964 | 6.2:-42975428 | None:intergenic |
| GTAGAGTCTGTTTGTGCTGC+TGG | 0.368390 | 6.2:+42968989 | MS.gene94303:CDS |
| TAGGTTTGTGTTTGTCTTTG+TGG | 0.376110 | 6.2:+42969260 | MS.gene94303:CDS |
| TTGACGAAGCAGAAGAGCTT+TGG | 0.377250 | 6.2:+42973557 | MS.gene94303:CDS |
| CTTGGTAGTGTTATTGTCTT+GGG | 0.377944 | 6.2:+42969019 | MS.gene94303:CDS |
| GTCTTTGTGGCCTGTGTCGT+TGG | 0.386000 | 6.2:+42969273 | MS.gene94303:CDS |
| GGTTTCCATGCCTATTAAAT+CGG | 0.386492 | 6.2:-42975407 | None:intergenic |
| GCTGATTACCTTAGGATGAA+TGG | 0.398954 | 6.2:+42967789 | MS.gene94303:CDS |
| CAATAATTGTCGTCTTTATG+AGG | 0.406702 | 6.2:+42969160 | MS.gene94303:CDS |
| TTGATCTCCTTATGCATACT+AGG | 0.406771 | 6.2:+42969241 | MS.gene94303:CDS |
| AAACTTTCGCTGATTACCTT+AGG | 0.409074 | 6.2:+42967781 | MS.gene94303:CDS |
| TAGTAGTTCTGTTGTCTCAA+TGG | 0.409178 | 6.2:+42975132 | MS.gene94303:CDS |
| ATGTATTTATAGGAGGTATT+GGG | 0.416308 | 6.2:+42968967 | MS.gene94303:intron |
| TTCCAAACTCGAGGATATCT+TGG | 0.417267 | 6.2:-42971748 | None:intergenic |
| AGAAGAGACTTCAGAGTTAA+CGG | 0.420834 | 6.2:+42975360 | MS.gene94303:CDS |
| AGGGGTTGAACTTGGAAAAT+CGG | 0.431982 | 6.2:-42967559 | None:intergenic |
| AAGGTATTTGCTGCTGCAAA+AGG | 0.436480 | 6.2:+42971718 | MS.gene94303:intron |
| CTCTAGAATACGAGAAAGAA+TGG | 0.450096 | 6.2:+42973241 | MS.gene94303:CDS |
| CTATATTCCCATTGTGGTGG+TGG | 0.450226 | 6.2:-42975217 | None:intergenic |
| GATATGTTTAACGATCGTTT+GGG | 0.451096 | 6.2:+42967348 | MS.gene94303:CDS |
| AAAATATCCACCACCACAAT+GGG | 0.452410 | 6.2:+42975210 | MS.gene94303:CDS |
| TGTGCTGCTGGTGCTATGCT+TGG | 0.453101 | 6.2:+42969001 | MS.gene94303:CDS |
| TGGAAAGAAGGGGTTGAACT+TGG | 0.456140 | 6.2:-42967567 | None:intergenic |
| ACTCGAGGATATCTTGGTCT+TGG | 0.456302 | 6.2:-42971742 | None:intergenic |
| ATAGAGAAAGAGGTGGTCAT+TGG | 0.456658 | 6.2:+42967391 | MS.gene94303:CDS |
| CTCTTGATACCTATGTCGCT+TGG | 0.459035 | 6.2:+42973175 | MS.gene94303:CDS |
| CCACTTCTACTATGGAAAGA+AGG | 0.459497 | 6.2:-42967579 | None:intergenic |
| CTGATTACCTTAGGATGAAT+GGG | 0.460628 | 6.2:+42967790 | MS.gene94303:CDS |
| TTTGTTGTAAATAGAGAAAG+AGG | 0.465563 | 6.2:+42967381 | MS.gene94303:CDS |
| TTATTGTCTTGGGATGTTCA+TGG | 0.466306 | 6.2:+42969029 | MS.gene94303:CDS |
| TTATGAGGGATTCGCATGCT+TGG | 0.472149 | 6.2:+42969175 | MS.gene94303:CDS |
| CATGTAGGTATTTGCAGACA+TGG | 0.476328 | 6.2:+42975090 | MS.gene94303:intron |
| GGACGATGAACAATTCGAAC+AGG | 0.477783 | 6.2:+42975381 | MS.gene94303:CDS |
| AGTTCTGTTGTCTCAATGGT+TGG | 0.480356 | 6.2:+42975136 | MS.gene94303:CDS |
| CACTTCTACTATGGAAAGAA+GGG | 0.481339 | 6.2:-42967578 | None:intergenic |
| TCTTTGTGGCCTGTGTCGTT+GGG | 0.482109 | 6.2:+42969274 | MS.gene94303:CDS |
| CCTTCTTTCCATAGTAGAAG+TGG | 0.483976 | 6.2:+42967579 | MS.gene94303:CDS |
| AATGGTTGGAAATGTCTTCA+AGG | 0.484077 | 6.2:+42975150 | MS.gene94303:CDS |
| CTTCAAGGAGCTAGGTATGA+TGG | 0.487411 | 6.2:+42975165 | MS.gene94303:CDS |
| ATATCTATCTACTGTAAAAG+AGG | 0.491255 | 6.2:+42973637 | MS.gene94303:CDS |
| CGATATTCATCTTACTGACA+TGG | 0.495794 | 6.2:+42967626 | MS.gene94303:CDS |
| GCCACTTAGGGAGCATATGC+AGG | 0.496923 | 6.2:+42969137 | MS.gene94303:intron |
| GATGATCTTGAGAACTATCA+TGG | 0.497299 | 6.2:+42967462 | MS.gene94303:CDS |
| ACACAAACCTAGTATGCATA+AGG | 0.497863 | 6.2:-42969248 | None:intergenic |
| CTTGCATGATAAGATGTTTG+AGG | 0.498325 | 6.2:+42973660 | MS.gene94303:CDS |
| TCTTGATACCTATGTCGCTT+GGG | 0.501458 | 6.2:+42973176 | MS.gene94303:CDS |
| AACAAAATGGATGTTAAGCA+AGG | 0.507980 | 6.2:+42973471 | MS.gene94303:CDS |
| AACTCAACCAAAACATCCTC+AGG | 0.508428 | 6.2:-42967489 | None:intergenic |
| TCTCGTTATACATCTAAACT+TGG | 0.517938 | 6.2:-42975466 | None:intergenic |
| ATTGAGACAACAGAACTACT+AGG | 0.527919 | 6.2:-42975130 | None:intergenic |
| AATAATTGTCGTCTTTATGA+GGG | 0.534051 | 6.2:+42969161 | MS.gene94303:CDS |
| ATTCATCCACTCATAGTGAA+AGG | 0.534451 | 6.2:+42967435 | MS.gene94303:CDS |
| AGATATACCCATTCATCCTA+AGG | 0.541122 | 6.2:-42967797 | None:intergenic |
| GCTCTTCTGCTTCGTCAACT+CGG | 0.545541 | 6.2:-42973552 | None:intergenic |
| AACAAATTTCCCAACGACAC+AGG | 0.550756 | 6.2:-42969283 | None:intergenic |
| TGATAGATACGTAAGAAAGC+AGG | 0.551062 | 6.2:+42975294 | MS.gene94303:CDS |
| TTCATCCACTCATAGTGAAA+GGG | 0.552865 | 6.2:+42967436 | MS.gene94303:CDS |
| GGTTGAGTTTCGTTATTATG+GGG | 0.553177 | 6.2:+42967503 | MS.gene94303:CDS |
| TCGAAACCAAAAGTTTCTTG+TGG | 0.554992 | 6.2:-42967606 | None:intergenic |
| TTCTAGTTCCCAAGCGACAT+AGG | 0.558781 | 6.2:-42973184 | None:intergenic |
| AATACGAGAAAGAATGGAAG+CGG | 0.559112 | 6.2:+42973247 | MS.gene94303:CDS |
| GGATGCCGATTTAATAGGCA+TGG | 0.559417 | 6.2:+42975402 | MS.gene94303:CDS |
| ATGTATCTATATTCCCATTG+TGG | 0.561013 | 6.2:-42975223 | None:intergenic |
| AAGGGTCAAGGAAAGACAAT+GGG | 0.563191 | 6.2:+42973490 | MS.gene94303:CDS |
| AGAATGGAAGCGGATAATAC+AGG | 0.564642 | 6.2:+42973257 | MS.gene94303:CDS |
| TTAAACATATCGACGAACGC+TGG | 0.565555 | 6.2:-42967336 | None:intergenic |
| TTATTAGTCTTCCAAACTCG+AGG | 0.571818 | 6.2:-42971757 | None:intergenic |
| ATCCACTCATAGTGAAAGGG+TGG | 0.575900 | 6.2:+42967439 | MS.gene94303:CDS |
| GGAAATGTCTTCAAGGAGCT+AGG | 0.581726 | 6.2:+42975157 | MS.gene94303:CDS |
| ATTACTTAATGCTTTAGCAG+AGG | 0.581952 | 6.2:+42973525 | MS.gene94303:CDS |
| TCCCACCCTTTCACTATGAG+TGG | 0.587518 | 6.2:-42967441 | None:intergenic |
| AAGTTTGTTTGATCGATGGA+GGG | 0.594586 | 6.2:+42967120 | None:intergenic |
| CGTGTAAGAATCAGGGTGCA+AGG | 0.595586 | 6.2:+42975256 | MS.gene94303:CDS |
| ACGAAATTCAGGCTTAAGAG+AGG | 0.595718 | 6.2:+42975327 | MS.gene94303:CDS |
| TGGGAATATAGATACATCAA+AGG | 0.609942 | 6.2:+42975229 | MS.gene94303:CDS |
| AGGAAAGCGTGTAAGAATCA+GGG | 0.610217 | 6.2:+42975249 | MS.gene94303:CDS |
| TATGGCTGTGTTCGTCTATG+TGG | 0.611511 | 6.2:+42969326 | MS.gene94303:CDS |
| TGGATGTTAAGCAAGGGTCA+AGG | 0.625921 | 6.2:+42973478 | MS.gene94303:CDS |
| TATCTATATTCCCATTGTGG+TGG | 0.627567 | 6.2:-42975220 | None:intergenic |
| ACAAAATGGATGTTAAGCAA+GGG | 0.632530 | 6.2:+42973472 | MS.gene94303:CDS |
| CAAGGGTCAAGGAAAGACAA+TGG | 0.634494 | 6.2:+42973489 | MS.gene94303:CDS |
| ACACAGCCATAATATGCAGA+AGG | 0.636985 | 6.2:-42969314 | None:intergenic |
| GTTGTAAATAGAGAAAGAGG+TGG | 0.641113 | 6.2:+42967384 | MS.gene94303:CDS |
| GAACTATCATGGATTTCCTG+AGG | 0.644616 | 6.2:+42967473 | MS.gene94303:CDS |
| TCCACTCATAGTGAAAGGGT+GGG | 0.654277 | 6.2:+42967440 | MS.gene94303:CDS |
| ACTTCTACTATGGAAAGAAG+GGG | 0.668936 | 6.2:-42967577 | None:intergenic |
| AAAGTTGTTAATGCAGTACA+CGG | 0.674701 | 6.2:+42973582 | MS.gene94303:CDS |
| GTAGGTATTTGCAGACATGG+AGG | 0.706718 | 6.2:+42975093 | MS.gene94303:intron |
| GCTGGATCGATTTCTGCATG+CGG | 0.709034 | 6.2:-42967318 | None:intergenic |
| ACAACAATAAAACTCGCGAG+AGG | 0.722901 | 6.2:-42967168 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 42967135 | 42975483 | 42967135 | ID=MS.gene94303 |
| chr6.2 | mRNA | 42967135 | 42975483 | 42967135 | ID=MS.gene94303.t1;Parent=MS.gene94303 |
| chr6.2 | exon | 42967135 | 42967206 | 42967135 | ID=MS.gene94303.t1.exon1;Parent=MS.gene94303.t1 |
| chr6.2 | CDS | 42967135 | 42967206 | 42967135 | ID=cds.MS.gene94303.t1;Parent=MS.gene94303.t1 |
| chr6.2 | exon | 42967318 | 42967668 | 42967318 | ID=MS.gene94303.t1.exon2;Parent=MS.gene94303.t1 |
| chr6.2 | CDS | 42967318 | 42967668 | 42967318 | ID=cds.MS.gene94303.t1;Parent=MS.gene94303.t1 |
| chr6.2 | exon | 42967771 | 42967811 | 42967771 | ID=MS.gene94303.t1.exon3;Parent=MS.gene94303.t1 |
| chr6.2 | CDS | 42967771 | 42967811 | 42967771 | ID=cds.MS.gene94303.t1;Parent=MS.gene94303.t1 |
| chr6.2 | exon | 42968979 | 42969053 | 42968979 | ID=MS.gene94303.t1.exon4;Parent=MS.gene94303.t1 |
| chr6.2 | CDS | 42968979 | 42969053 | 42968979 | ID=cds.MS.gene94303.t1;Parent=MS.gene94303.t1 |
| chr6.2 | exon | 42969146 | 42969347 | 42969146 | ID=MS.gene94303.t1.exon5;Parent=MS.gene94303.t1 |
| chr6.2 | CDS | 42969146 | 42969347 | 42969146 | ID=cds.MS.gene94303.t1;Parent=MS.gene94303.t1 |
| chr6.2 | exon | 42971721 | 42971822 | 42971721 | ID=MS.gene94303.t1.exon6;Parent=MS.gene94303.t1 |
| chr6.2 | CDS | 42971721 | 42971822 | 42971721 | ID=cds.MS.gene94303.t1;Parent=MS.gene94303.t1 |
| chr6.2 | exon | 42973135 | 42973278 | 42973135 | ID=MS.gene94303.t1.exon7;Parent=MS.gene94303.t1 |
| chr6.2 | CDS | 42973135 | 42973278 | 42973135 | ID=cds.MS.gene94303.t1;Parent=MS.gene94303.t1 |
| chr6.2 | exon | 42973469 | 42973681 | 42973469 | ID=MS.gene94303.t1.exon8;Parent=MS.gene94303.t1 |
| chr6.2 | CDS | 42973469 | 42973681 | 42973469 | ID=cds.MS.gene94303.t1;Parent=MS.gene94303.t1 |
| chr6.2 | exon | 42975097 | 42975483 | 42975097 | ID=MS.gene94303.t1.exon9;Parent=MS.gene94303.t1 |
| chr6.2 | CDS | 42975097 | 42975483 | 42975097 | ID=cds.MS.gene94303.t1;Parent=MS.gene94303.t1 |
| Gene Sequence |
| Protein sequence |
