Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94337.t1 | XP_024633642.1 | 80.7 | 140 | 23 | 1 | 32 | 171 | 4 | 139 | 3.70E-52 | 214.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94337.t1 | A0A396IRE0 | 78.6 | 131 | 24 | 1 | 41 | 171 | 1 | 127 | 1.3e-46 | 195.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049194 | MS.gene94337 | 0.822262 | 2.60E-53 | -1.69E-46 |
| MS.gene055359 | MS.gene94337 | 0.828833 | 7.22E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTGGCGTAGTTGAAATTA+TGG | 0.312736 | 3.2:-43324497 | MS.gene94337:CDS |
| CATCGTTAGAGTTAGTTTCA+TGG | 0.341480 | 3.2:+43324290 | None:intergenic |
| TTCAAGCGCACACATGATTT+CGG | 0.347261 | 3.2:+43324333 | None:intergenic |
| CGATGAAAATGCATTACTTA+TGG | 0.347851 | 3.2:-43324272 | MS.gene94337:CDS |
| CGCAAGTTGTTGAGGGGTTG+TGG | 0.348606 | 3.2:-43324231 | MS.gene94337:CDS |
| TTGTTGAGGGGTTGTGGGAG+AGG | 0.395189 | 3.2:-43324225 | MS.gene94337:CDS |
| CTTATTACTTGTAGCAATGA+AGG | 0.409492 | 3.2:-43324434 | MS.gene94337:CDS |
| GTTGTGGGAGAGGCTTTCTA+TGG | 0.410974 | 3.2:-43324215 | MS.gene94337:CDS |
| AACACTGCTTGGAGCATAGC+TGG | 0.467684 | 3.2:-43324106 | None:intergenic |
| GTAAAGGAATCTAACAAGAA+AGG | 0.492418 | 3.2:-43324457 | MS.gene94337:CDS |
| GCAAGTTGTTGAGGGGTTGT+GGG | 0.506150 | 3.2:-43324230 | MS.gene94337:CDS |
| AACACAACGAAAACACTGCT+TGG | 0.517796 | 3.2:-43324117 | MS.gene94337:CDS |
| AGAGCTTAACTCAAAAGTAA+AGG | 0.520218 | 3.2:-43324473 | MS.gene94337:CDS |
| AGACAACCGCAAGTTGTTGA+GGG | 0.537910 | 3.2:-43324238 | MS.gene94337:CDS |
| TAGACAACCGCAAGTTGTTG+AGG | 0.551855 | 3.2:-43324239 | MS.gene94337:CDS |
| CACAACCCCTCAACAACTTG+CGG | 0.571050 | 3.2:+43324232 | None:intergenic |
| GAAGGAATTCAAGAAATGAA+AGG | 0.582757 | 3.2:-43324568 | MS.gene94337:CDS |
| TATTTGCGTGCAGCAAAACA+TGG | 0.589012 | 3.2:-43324361 | MS.gene94337:CDS |
| AAATCAACACAAGAGAGAGA+AGG | 0.626440 | 3.2:-43324586 | MS.gene94337:CDS |
| GACAACCGCAAGTTGTTGAG+GGG | 0.642150 | 3.2:-43324237 | MS.gene94337:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGAAAAAGATAAGACATTTT+TGG | - | chr3.2:43324177-43324196 | MS.gene94337:CDS | 20.0% |
| ! | ATGAAAGGTTCAACAAAAAA+TGG | - | chr3.2:43324154-43324173 | MS.gene94337:CDS | 25.0% |
| ! | GAATTATCAGCAAAGAAAAA+TGG | - | chr3.2:43324307-43324326 | MS.gene94337:CDS | 25.0% |
| AGAGCTTAACTCAAAAGTAA+AGG | - | chr3.2:43324234-43324253 | MS.gene94337:CDS | 30.0% | |
| CGATGAAAATGCATTACTTA+TGG | - | chr3.2:43324435-43324454 | MS.gene94337:CDS | 30.0% | |
| CTTATTACTTGTAGCAATGA+AGG | - | chr3.2:43324273-43324292 | MS.gene94337:CDS | 30.0% | |
| GAAGGAATTCAAGAAATGAA+AGG | - | chr3.2:43324139-43324158 | MS.gene94337:CDS | 30.0% | |
| GTAAAGGAATCTAACAAGAA+AGG | - | chr3.2:43324250-43324269 | MS.gene94337:CDS | 30.0% | |
| AAATCAACACAAGAGAGAGA+AGG | - | chr3.2:43324121-43324140 | MS.gene94337:CDS | 35.0% | |
| AAGTGGCGTAGTTGAAATTA+TGG | - | chr3.2:43324210-43324229 | MS.gene94337:CDS | 35.0% | |
| ! | CATCGTTAGAGTTAGTTTCA+TGG | + | chr3.2:43324420-43324439 | None:intergenic | 35.0% |
| AACACAACGAAAACACTGCT+TGG | - | chr3.2:43324590-43324609 | MS.gene94337:CDS | 40.0% | |
| TATTTGCGTGCAGCAAAACA+TGG | - | chr3.2:43324346-43324365 | MS.gene94337:CDS | 40.0% | |
| TTCAAGCGCACACATGATTT+CGG | + | chr3.2:43324377-43324396 | None:intergenic | 40.0% | |
| !! | TTTTTGGCAGTAGCGAAAAG+TGG | - | chr3.2:43324193-43324212 | MS.gene94337:CDS | 40.0% |
| AGACAACCGCAAGTTGTTGA+GGG | - | chr3.2:43324469-43324488 | MS.gene94337:CDS | 45.0% | |
| TAGACAACCGCAAGTTGTTG+AGG | - | chr3.2:43324468-43324487 | MS.gene94337:CDS | 45.0% | |
| CACAACCCCTCAACAACTTG+CGG | + | chr3.2:43324478-43324497 | None:intergenic | 50.0% | |
| GACAACCGCAAGTTGTTGAG+GGG | - | chr3.2:43324470-43324489 | MS.gene94337:CDS | 50.0% | |
| GCAAGTTGTTGAGGGGTTGT+GGG | - | chr3.2:43324477-43324496 | MS.gene94337:CDS | 50.0% | |
| GTTGTGGGAGAGGCTTTCTA+TGG | - | chr3.2:43324492-43324511 | MS.gene94337:CDS | 50.0% | |
| CACTGCTTGGAGCATAGCTG+AGG | - | chr3.2:43324603-43324622 | MS.gene94337:CDS | 55.0% | |
| CGCAAGTTGTTGAGGGGTTG+TGG | - | chr3.2:43324476-43324495 | MS.gene94337:CDS | 55.0% | |
| TTGTTGAGGGGTTGTGGGAG+AGG | - | chr3.2:43324482-43324501 | MS.gene94337:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 43324107 | 43324622 | 43324107 | ID=MS.gene94337 |
| chr3.2 | mRNA | 43324107 | 43324622 | 43324107 | ID=MS.gene94337.t1;Parent=MS.gene94337 |
| chr3.2 | exon | 43324107 | 43324622 | 43324107 | ID=MS.gene94337.t1.exon1;Parent=MS.gene94337.t1 |
| chr3.2 | CDS | 43324107 | 43324622 | 43324107 | ID=cds.MS.gene94337.t1;Parent=MS.gene94337.t1 |
| Gene Sequence |
| Protein sequence |
