Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049194.t1 | RHN70040.1 | 82.6 | 138 | 17 | 1 | 1 | 131 | 1 | 138 | 8.00E-55 | 223 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049194.t1 | A0A396IX51 | 82.6 | 138 | 17 | 1 | 1 | 131 | 1 | 138 | 5.8e-55 | 223.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049194 | MS.gene052187 | 0.811601 | 6.45E-51 | -1.69E-46 |
| MS.gene049194 | MS.gene055359 | 0.811481 | 6.85E-51 | -1.69E-46 |
| MS.gene049194 | MS.gene06310 | 0.802017 | 6.85E-49 | -1.69E-46 |
| MS.gene049194 | MS.gene68655 | 0.829262 | 5.68E-55 | -1.69E-46 |
| MS.gene049194 | MS.gene68821 | 0.829367 | 5.36E-55 | -1.69E-46 |
| MS.gene049194 | MS.gene90334 | 0.821141 | 4.73E-53 | -1.69E-46 |
| MS.gene049194 | MS.gene94337 | 0.822262 | 2.60E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049194.t1 | MTR_3g098180 | 88.288 | 111 | 13 | 0 | 1 | 111 | 1 | 111 | 1.46e-70 | 209 |
| MS.gene049194.t1 | MTR_3g098170 | 75.000 | 116 | 26 | 1 | 4 | 119 | 9 | 121 | 1.47e-59 | 180 |
| MS.gene049194.t1 | MTR_3g098160 | 69.355 | 124 | 30 | 2 | 1 | 119 | 1 | 121 | 1.71e-56 | 172 |
| MS.gene049194.t1 | MTR_3g098170 | 75.000 | 108 | 24 | 1 | 4 | 111 | 9 | 113 | 7.64e-55 | 169 |
| MS.gene049194.t1 | MTR_3g098160 | 68.103 | 116 | 29 | 2 | 1 | 111 | 1 | 113 | 3.75e-51 | 160 |
| MS.gene049194.t1 | MTR_3g098130 | 66.355 | 107 | 36 | 0 | 4 | 110 | 9 | 115 | 2.16e-47 | 150 |
| MS.gene049194.t1 | MTR_3g098130 | 66.355 | 107 | 36 | 0 | 4 | 110 | 47 | 153 | 3.78e-47 | 151 |
| MS.gene049194.t1 | MTR_3g098120 | 60.000 | 110 | 36 | 2 | 2 | 111 | 48 | 149 | 4.76e-40 | 132 |
| MS.gene049194.t1 | MTR_3g098140 | 64.706 | 102 | 30 | 2 | 15 | 111 | 9 | 109 | 6.06e-39 | 129 |
| MS.gene049194.t1 | MTR_3g098100 | 61.468 | 109 | 38 | 3 | 5 | 111 | 2 | 108 | 7.95e-37 | 123 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049194.t1 | AT1G04290 | 50.806 | 124 | 59 | 2 | 5 | 127 | 2 | 124 | 4.28e-34 | 115 |
| MS.gene049194.t1 | AT1G04290 | 54.128 | 109 | 44 | 3 | 5 | 110 | 2 | 107 | 3.83e-31 | 108 |
Find 37 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAATTTCCTCCGAAGTTTC+TGG | 0.138608 | 3.3:-80798181 | MS.gene049194:CDS |
| TGAATTAACTTGCAGAATTC+AGG | 0.239073 | 3.3:-80797972 | MS.gene049194:intron |
| GTTTCTGGAACATCTAATCT+TGG | 0.344011 | 3.3:-80798166 | MS.gene049194:CDS |
| GAAAGGAGAAGCGTCGTTAA+AGG | 0.351047 | 3.3:-80798214 | MS.gene049194:CDS |
| GCTGGATTTCAGTTGGATAC+TGG | 0.394930 | 3.3:-80797882 | MS.gene049194:CDS |
| ACGTCTTCAGTATGTTAAAC+AGG | 0.429052 | 3.3:+80797756 | None:intergenic |
| AAGAGGTACTTGGAGGAGAA+AGG | 0.429137 | 3.3:-80798231 | MS.gene049194:CDS |
| CGCTTGGATCTCATTGAGCC+TGG | 0.445136 | 3.3:-80798135 | MS.gene049194:CDS |
| AATCTTGGGTGGCTTACGCT+TGG | 0.453352 | 3.3:-80798151 | MS.gene049194:CDS |
| TCAATCGCAATTCCCGCCGC+TGG | 0.459843 | 3.3:-80797900 | MS.gene049194:CDS |
| TTTCTGGAACATCTAATCTT+GGG | 0.471271 | 3.3:-80798165 | MS.gene049194:CDS |
| GGAATCAGTGAAGAGGTACT+TGG | 0.484190 | 3.3:-80798241 | MS.gene049194:CDS |
| TCCCGCCGCTGGATTTCAGT+TGG | 0.485139 | 3.3:-80797889 | MS.gene049194:CDS |
| GCATGAGACATTGATCTCAA+TGG | 0.492399 | 3.3:+80797854 | None:intergenic |
| CATCATTACTCTGGTGGATG+TGG | 0.494972 | 3.3:-80797928 | MS.gene049194:CDS |
| TGAGATCAATGTCTCATGCT+TGG | 0.497702 | 3.3:-80797850 | MS.gene049194:CDS |
| CACATCCACCAGAGTAATGA+TGG | 0.509226 | 3.3:+80797929 | None:intergenic |
| CTGGAACATCTAATCTTGGG+TGG | 0.511525 | 3.3:-80798162 | MS.gene049194:CDS |
| ATTGAGAAAACGACACGGCC+AGG | 0.517820 | 3.3:+80798117 | None:intergenic |
| TCCAACTGAAATCCAGCGGC+GGG | 0.545347 | 3.3:+80797888 | None:intergenic |
| ATTAGATGTTCCAGAAACTT+CGG | 0.547234 | 3.3:+80798171 | None:intergenic |
| TGGTGCCATCATTACTCTGG+TGG | 0.548725 | 3.3:-80797934 | MS.gene049194:CDS |
| CCTTGGTGCCATCATTACTC+TGG | 0.553351 | 3.3:-80797937 | MS.gene049194:CDS |
| ATTACTCTGGTGGATGTGGT+AGG | 0.556926 | 3.3:-80797924 | MS.gene049194:CDS |
| ATCAGTGAAGAGGTACTTGG+AGG | 0.578355 | 3.3:-80798238 | MS.gene049194:CDS |
| TCAGGTACGTACTTGCACCT+TGG | 0.609189 | 3.3:-80797954 | MS.gene049194:CDS |
| CAGATTTGGAATCAGTGAAG+AGG | 0.627422 | 3.3:-80798248 | MS.gene049194:CDS |
| AGCTTTGAAATACACTAACA+TGG | 0.640226 | 3.3:+80797814 | None:intergenic |
| ATCCAACTGAAATCCAGCGG+CGG | 0.646543 | 3.3:+80797887 | None:intergenic |
| AGATGTTCCAGAAACTTCGG+AGG | 0.661872 | 3.3:+80798174 | None:intergenic |
| AGAGTACGAACGAGTAAACG+TGG | 0.662463 | 3.3:+80798084 | None:intergenic |
| CCAGAGTAATGATGGCACCA+AGG | 0.663656 | 3.3:+80797937 | None:intergenic |
| GAGTACGAACGAGTAAACGT+GGG | 0.664966 | 3.3:+80798085 | None:intergenic |
| AGTACGAACGAGTAAACGTG+GGG | 0.696588 | 3.3:+80798086 | None:intergenic |
| AGTATCCAACTGAAATCCAG+CGG | 0.715519 | 3.3:+80797884 | None:intergenic |
| TGTTCATTGAGAAAACGACA+CGG | 0.773076 | 3.3:+80798112 | None:intergenic |
| GTACGAACGAGTAAACGTGG+GGG | 0.804780 | 3.3:+80798087 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTAGATGTTCCAGAAACTT+CGG | + | chr3.3:80797860-80797879 | None:intergenic | 30.0% | |
| !! | AGCTTTGAAATACACTAACA+TGG | + | chr3.3:80798217-80798236 | None:intergenic | 30.0% |
| !! | TGAATTAACTTGCAGAATTC+AGG | - | chr3.3:80798056-80798075 | MS.gene049194:intron | 30.0% |
| !! | TTTCTGGAACATCTAATCTT+GGG | - | chr3.3:80797863-80797882 | MS.gene049194:CDS | 30.0% |
| TAAATTTCCTCCGAAGTTTC+TGG | - | chr3.3:80797847-80797866 | MS.gene049194:CDS | 35.0% | |
| TGTTCATTGAGAAAACGACA+CGG | + | chr3.3:80797919-80797938 | None:intergenic | 35.0% | |
| !! | GTTTCTGGAACATCTAATCT+TGG | - | chr3.3:80797862-80797881 | MS.gene049194:CDS | 35.0% |
| AGTATCCAACTGAAATCCAG+CGG | + | chr3.3:80798147-80798166 | None:intergenic | 40.0% | |
| GCATGAGACATTGATCTCAA+TGG | + | chr3.3:80798177-80798196 | None:intergenic | 40.0% | |
| TGAGATCAATGTCTCATGCT+TGG | - | chr3.3:80798178-80798197 | MS.gene049194:CDS | 40.0% | |
| ! | CAGATTTGGAATCAGTGAAG+AGG | - | chr3.3:80797780-80797799 | MS.gene049194:CDS | 40.0% |
| AGAGTACGAACGAGTAAACG+TGG | + | chr3.3:80797947-80797966 | None:intergenic | 45.0% | |
| AGATGTTCCAGAAACTTCGG+AGG | + | chr3.3:80797857-80797876 | None:intergenic | 45.0% | |
| AGTACGAACGAGTAAACGTG+GGG | + | chr3.3:80797945-80797964 | None:intergenic | 45.0% | |
| CACATCCACCAGAGTAATGA+TGG | + | chr3.3:80798102-80798121 | None:intergenic | 45.0% | |
| CTGGAACATCTAATCTTGGG+TGG | - | chr3.3:80797866-80797885 | MS.gene049194:CDS | 45.0% | |
| GAGTACGAACGAGTAAACGT+GGG | + | chr3.3:80797946-80797965 | None:intergenic | 45.0% | |
| GCTGGATTTCAGTTGGATAC+TGG | - | chr3.3:80798146-80798165 | MS.gene049194:CDS | 45.0% | |
| ! | ATTACTCTGGTGGATGTGGT+AGG | - | chr3.3:80798104-80798123 | MS.gene049194:CDS | 45.0% |
| ! | CATCATTACTCTGGTGGATG+TGG | - | chr3.3:80798100-80798119 | MS.gene049194:CDS | 45.0% |
| !! | AAGAGGTACTTGGAGGAGAA+AGG | - | chr3.3:80797797-80797816 | MS.gene049194:CDS | 45.0% |
| !! | ATCAGTGAAGAGGTACTTGG+AGG | - | chr3.3:80797790-80797809 | MS.gene049194:CDS | 45.0% |
| !! | GAAAGGAGAAGCGTCGTTAA+AGG | - | chr3.3:80797814-80797833 | MS.gene049194:CDS | 45.0% |
| !! | GGAATCAGTGAAGAGGTACT+TGG | - | chr3.3:80797787-80797806 | MS.gene049194:CDS | 45.0% |
| AATCTTGGGTGGCTTACGCT+TGG | - | chr3.3:80797877-80797896 | MS.gene049194:CDS | 50.0% | |
| ATCCAACTGAAATCCAGCGG+CGG | + | chr3.3:80798144-80798163 | None:intergenic | 50.0% | |
| ATTGAGAAAACGACACGGCC+AGG | + | chr3.3:80797914-80797933 | None:intergenic | 50.0% | |
| CCAGAGTAATGATGGCACCA+AGG | + | chr3.3:80798094-80798113 | None:intergenic | 50.0% | |
| GTACGAACGAGTAAACGTGG+GGG | + | chr3.3:80797944-80797963 | None:intergenic | 50.0% | |
| TCAGGTACGTACTTGCACCT+TGG | - | chr3.3:80798074-80798093 | MS.gene049194:intron | 50.0% | |
| !! | CCTTGGTGCCATCATTACTC+TGG | - | chr3.3:80798091-80798110 | MS.gene049194:intron | 50.0% |
| !! | TGGTGCCATCATTACTCTGG+TGG | - | chr3.3:80798094-80798113 | MS.gene049194:intron | 50.0% |
| CGCTTGGATCTCATTGAGCC+TGG | - | chr3.3:80797893-80797912 | MS.gene049194:CDS | 55.0% | |
| TCCAACTGAAATCCAGCGGC+GGG | + | chr3.3:80798143-80798162 | None:intergenic | 55.0% | |
| TCAATCGCAATTCCCGCCGC+TGG | - | chr3.3:80798128-80798147 | MS.gene049194:CDS | 60.0% | |
| TCCCGCCGCTGGATTTCAGT+TGG | - | chr3.3:80798139-80798158 | MS.gene049194:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 80797770 | 80798280 | 80797770 | ID=MS.gene049194 |
| chr3.3 | mRNA | 80797770 | 80798280 | 80797770 | ID=MS.gene049194.t1;Parent=MS.gene049194 |
| chr3.3 | exon | 80798095 | 80798280 | 80798095 | ID=MS.gene049194.t1.exon1;Parent=MS.gene049194.t1 |
| chr3.3 | CDS | 80798095 | 80798280 | 80798095 | ID=cds.MS.gene049194.t1;Parent=MS.gene049194.t1 |
| chr3.3 | exon | 80797770 | 80797979 | 80797770 | ID=MS.gene049194.t1.exon2;Parent=MS.gene049194.t1 |
| chr3.3 | CDS | 80797770 | 80797979 | 80797770 | ID=cds.MS.gene049194.t1;Parent=MS.gene049194.t1 |
| Gene Sequence |
| Protein sequence |
