AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94737

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94737.t1 MTR_8g064590 97.037 135 4 0 231 365 1 135 5.21e-92 271
MS.gene94737.t1 MTR_5g034290 67.606 142 46 0 224 365 27 168 1.35e-64 203
MS.gene94737.t1 MTR_5g034290 69.630 135 41 0 231 365 1 135 1.29e-63 199
MS.gene94737.t1 MTR_2g014360 66.423 137 40 2 235 365 14 150 1.30e-59 189
MS.gene94737.t1 MTR_2g090635 68.702 131 37 1 239 365 8 138 7.40e-57 182
MS.gene94737.t1 MTR_6g060020 84.848 99 14 1 267 365 1 98 1.25e-51 167
MS.gene94737.t1 MTR_5g083900 48.800 125 55 2 241 364 111 227 1.08e-28 111
MS.gene94737.t1 MTR_5g083870 47.287 129 55 4 241 364 5 125 5.99e-25 98.6
MS.gene94737.t1 MTR_2g090640 55.000 80 32 1 284 359 456 535 4.22e-16 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94737.t1 AT1G23750 74.265 136 34 1 231 365 1 136 4.98e-72 221
MS.gene94737.t1 AT1G10590 76.642 137 29 2 231 364 15 151 8.80e-71 218
MS.gene94737.t1 AT1G10590 76.642 137 29 2 231 364 1 137 9.63e-71 218
MS.gene94737.t1 AT1G10590 76.642 137 29 2 231 364 1 137 9.63e-71 218
MS.gene94737.t1 AT2G33845 71.875 128 35 1 239 365 53 180 1.55e-57 185
MS.gene94737.t1 AT4G28440 66.667 132 38 3 239 364 18 149 6.33e-55 177
MS.gene94737.t1 AT1G23750 77.778 99 22 0 267 365 1 99 1.59e-52 169
MS.gene94737.t1 AT1G03810 61.417 127 46 2 239 362 15 141 1.91e-47 158
MS.gene94737.t1 AT4G13870 35.065 154 95 4 66 217 128 278 1.09e-19 88.6
MS.gene94737.t1 AT4G13870 35.065 154 95 4 66 217 128 278 1.65e-19 87.8
MS.gene94737.t1 AT2G36110 35.256 156 91 4 61 214 51 198 2.31e-17 80.9
MS.gene94737.t1 AT3G12410 28.037 214 126 8 26 214 12 222 1.01e-16 79.0
MS.gene94737.t1 AT3G12460 25.893 224 128 7 26 214 11 231 8.74e-15 73.6
MS.gene94737.t1 AT3G12430 31.915 141 93 3 76 214 91 230 6.51e-14 71.2
MS.gene94737.t1 AT3G12470 31.646 158 104 4 59 214 49 204 1.13e-12 67.0
MS.gene94737.t1 AT3G12440 31.176 170 109 5 54 216 179 347 4.73e-12 67.0
MS.gene94737.t1 AT3G12420 28.994 169 78 5 26 153 11 178 6.35e-12 64.3

Find 127 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGTTTCACTTCCAGTTTC+AGG 0.184004 7.1:-35387198 MS.gene94737:intron
GGGATAATACTTGGCTTGAT+TGG 0.261654 7.1:-35387589 MS.gene94737:CDS
CCAGTTTCAGGTTAAGATTA+TGG 0.295750 7.1:-35387186 MS.gene94737:CDS
TCCTGGCCGCGTTGGGAGTT+TGG 0.310200 7.1:+35387932 None:intergenic
AGTGGATTACGAGGAGTAGA+TGG 0.335791 7.1:-35387610 MS.gene94737:CDS
GGGAACAGGTGAGGAGGGTT+TGG 0.335938 7.1:+35388032 None:intergenic
AAATTAGCGGAACGTGTTCT+TGG 0.338136 7.1:-35387653 MS.gene94737:CDS
ACGCCGACGAATCTGTTGTT+AGG 0.344345 7.1:+35387803 None:intergenic
AGGAAGGTGAAGAGGGAAGT+GGG 0.344657 7.1:+35387833 None:intergenic
AGTTTGGTTGGGGATGAGAC+TGG 0.358059 7.1:-35386999 MS.gene94737:CDS
ATGTCGAGGCCGATGATTGC+GGG 0.358531 7.1:+35387965 None:intergenic
CCATAATCTTAACCTGAAAC+TGG 0.365342 7.1:+35387186 None:intergenic
AGGGTGTTGGTGTGGAGTTT+TGG 0.365688 7.1:+35388137 None:intergenic
TCCTACTGATTTCACTGTTA+AGG 0.375855 7.1:-35386832 MS.gene94737:CDS
TGGGTTATCCTGGCCGCGTT+GGG 0.389115 7.1:+35387925 None:intergenic
GGTGAAGAGGGAAGTGGGGA+TGG 0.395784 7.1:+35387838 None:intergenic
GCCAGGTGTCGACGTGGGTG+GGG 0.398494 7.1:+35388011 None:intergenic
GATGCGAATTGCTGAGAGTT+TGG 0.401501 7.1:-35387015 MS.gene94737:CDS
GTAGGTGTTGTGGGTGTCGT+AGG 0.407318 7.1:+35388084 None:intergenic
GGATAATACTTGGCTTGATT+GGG 0.410346 7.1:-35387588 MS.gene94737:CDS
GAGGAAGGTGAAGAGGGAAG+TGG 0.420070 7.1:+35387832 None:intergenic
GTTAGAGAGGAAGGTGAAGA+GGG 0.423894 7.1:+35387826 None:intergenic
GAGTTGCGGCTGGGTTATCC+TGG 0.424451 7.1:+35387915 None:intergenic
AGCCAGGTGTCGACGTGGGT+GGG 0.426262 7.1:+35388010 None:intergenic
AAGAGGGAAGTGGGGATGGA+AGG 0.428328 7.1:+35387842 None:intergenic
GTTGTTAGGGTTAGAGAGGA+AGG 0.431930 7.1:+35387817 None:intergenic
TACTTGGCTTGATTGGGATC+AGG 0.432986 7.1:-35387582 MS.gene94737:CDS
TAAGGTCGAACAGCTTCAAC+CGG 0.434832 7.1:-35387126 MS.gene94737:CDS
TAGGTGTTGTGGGTGTCGTA+GGG 0.434919 7.1:+35388085 None:intergenic
AATTAGCGGAACGTGTTCTT+GGG 0.439595 7.1:-35387652 MS.gene94737:CDS
CAGAGCTGGAGAGTTGCGGC+TGG 0.445912 7.1:+35387905 None:intergenic
CCAGGTGTCGACGTGGGTGG+GGG 0.446045 7.1:+35388012 None:intergenic
AGCGGTTGTTTGTGCAGAGC+TGG 0.450077 7.1:+35387891 None:intergenic
AGTGGATGAGATGGAAGATA+AGG 0.451037 7.1:+35387867 None:intergenic
ATTATGGCGGAGGCAAAACC+TGG 0.453548 7.1:-35387170 MS.gene94737:CDS
TGAGGCTTGCTGTGGACAAA+TGG 0.453565 7.1:-35386875 MS.gene94737:CDS
TCCAAACTCCCAACGCGGCC+AGG 0.456447 7.1:-35387933 MS.gene94737:CDS
CGAATTGCTGAGAGTTTGGT+TGG 0.459494 7.1:-35387011 MS.gene94737:CDS
GAGCCAGGTGTCGACGTGGG+TGG 0.461261 7.1:+35388009 None:intergenic
GGAAGGTGAAGAGGGAAGTG+GGG 0.462479 7.1:+35387834 None:intergenic
CTGGGTTATCCTGGCCGCGT+TGG 0.465522 7.1:+35387924 None:intergenic
GAGGGTTTGGATGGTGAAGG+TGG 0.465836 7.1:+35388045 None:intergenic
GGTGATGGAGTAGGTGTTGT+GGG 0.466954 7.1:+35388075 None:intergenic
TCGTCGTCTTCACTCTCATC+AGG 0.469464 7.1:-35387516 MS.gene94737:intron
CTGACGAGGCTGAGGACCAT+TGG 0.471346 7.1:+35387037 None:intergenic
TGGCCACACTCTGACTGTTA+AGG 0.471428 7.1:-35387096 MS.gene94737:CDS
ATCGTGATTTGTTGAAAAGT+GGG 0.476610 7.1:-35387682 MS.gene94737:CDS
GATGTTGAAGGGTGTTGGTG+TGG 0.478561 7.1:+35388129 None:intergenic
TGGAAGGAGAGTGGATGAGA+TGG 0.481033 7.1:+35387858 None:intergenic
AAGTGGGGATGGAAGGAGAG+TGG 0.482199 7.1:+35387849 None:intergenic
GAGGCTTGCTGTGGACAAAT+GGG 0.484723 7.1:-35386874 MS.gene94737:CDS
CCTTGCTGCTGAGGTGCTTG+AGG 0.487145 7.1:-35387705 MS.gene94737:CDS
GTCGATGAGGGTGATGTTGA+AGG 0.488166 7.1:+35388117 None:intergenic
GGTTAGAGAGGAAGGTGAAG+AGG 0.488884 7.1:+35387825 None:intergenic
GTTTGGTTGGGGATGAGACT+GGG 0.491245 7.1:-35386998 MS.gene94737:CDS
TGACGAGGCTGAGGACCATT+GGG 0.503537 7.1:+35387038 None:intergenic
CGCCGACGAATCTGTTGTTA+GGG 0.505935 7.1:+35387804 None:intergenic
TTCTATAAGCTTCTCGATGT+CGG 0.508461 7.1:+35387769 None:intergenic
GGATTGAGTTTCGAGGAGCC+AGG 0.512276 7.1:+35387994 None:intergenic
AAGGTCGGCTTTCTCAATCC+AGG 0.513274 7.1:+35387152 None:intergenic
GATCGTGATTTGTTGAAAAG+TGG 0.513477 7.1:-35387683 MS.gene94737:CDS
AGGGTGATGTTGAAGGGTGT+TGG 0.516201 7.1:+35388124 None:intergenic
AACAACAGATTCGTCGGCGT+CGG 0.521128 7.1:-35387800 MS.gene94737:CDS
GAAGGTGGTGTCGATGGTGA+TGG 0.522246 7.1:+35388060 None:intergenic
GAATTGCTGAGAGTTTGGTT+GGG 0.529078 7.1:-35387010 MS.gene94737:CDS
CAAGTCGATCTGCTGAAAGA+TGG 0.529778 7.1:-35386948 MS.gene94737:CDS
GGAGTAGATGGGATAATACT+TGG 0.535209 7.1:-35387598 MS.gene94737:CDS
TCAGGTTAAGATTATGGCGG+AGG 0.536907 7.1:-35387180 MS.gene94737:CDS
CAGCTTCAACCGGGGACTAG+TGG 0.540664 7.1:-35387116 MS.gene94737:CDS
ATGCAGAAGGGTCGTCCCAA+TGG 0.541607 7.1:-35387053 MS.gene94737:CDS
AATTGCTGAGAGTTTGGTTG+GGG 0.542598 7.1:-35387009 MS.gene94737:CDS
CTACTCCTCGTAATCCACTT+TGG 0.553491 7.1:+35387614 None:intergenic
ATTCGCATCTGACGAGGCTG+AGG 0.553923 7.1:+35387029 None:intergenic
TCATCGGCCTCGACATCGAA+TGG 0.559761 7.1:-35387958 MS.gene94737:CDS
ACAGGTGAGGAGGGTTTGGA+TGG 0.561081 7.1:+35388036 None:intergenic
TACTAAGATGGTGATGCAGA+AGG 0.561427 7.1:-35387066 MS.gene94737:CDS
ATCTGTTGTTAGGGTTAGAG+AGG 0.563682 7.1:+35387813 None:intergenic
GAGAAAGCCGACCTTCACTA+AGG 0.566200 7.1:-35387144 MS.gene94737:CDS
GATGGTGAAGGTGGTGTCGA+TGG 0.566837 7.1:+35388054 None:intergenic
GTTCATATTCAACAAGGGAC+AGG 0.567897 7.1:+35386799 None:intergenic
TTTGCGTAACCTTGCTGCTG+AGG 0.567929 7.1:-35387714 MS.gene94737:CDS
TCGATGAGGGTGATGTTGAA+GGG 0.568102 7.1:+35388118 None:intergenic
AACCCTAACAACAGATTCGT+CGG 0.574849 7.1:-35387806 MS.gene94737:CDS
GGTGTCGATGGTGATGGAGT+AGG 0.574890 7.1:+35388066 None:intergenic
TGTCGAGGCCGATGATTGCG+GGG 0.579688 7.1:+35387966 None:intergenic
TGGTGATGGAGTAGGTGTTG+TGG 0.580668 7.1:+35388074 None:intergenic
AGGTGTTGTGGGTGTCGTAG+GGG 0.584862 7.1:+35388086 None:intergenic
GGGAGAGGGATTGAGTTTCG+AGG 0.587929 7.1:+35387987 None:intergenic
AGAGCTGGAGAGTTGCGGCT+GGG 0.589632 7.1:+35387906 None:intergenic
ATATGAACTTGTTAATGTTG+TGG 0.589925 7.1:-35386784 MS.gene94737:CDS
TGTTCGACCTTAGTGAAGGT+CGG 0.595402 7.1:+35387137 None:intergenic
TCCTTAACAGTGAAATCAGT+AGG 0.595652 7.1:+35386831 None:intergenic
AAGGTCGAACAGCTTCAACC+GGG 0.597345 7.1:-35387125 MS.gene94737:CDS
TGTTGTGGGTGTCGTAGGGG+AGG 0.597459 7.1:+35388089 None:intergenic
AGAGTGTGGCCACTAGTCCC+CGG 0.599002 7.1:+35387107 None:intergenic
GAGGAGGGTTTGGATGGTGA+AGG 0.599653 7.1:+35388042 None:intergenic
GTGGATTACGAGGAGTAGAT+GGG 0.602513 7.1:-35387609 MS.gene94737:CDS
CGAGGAGCCAGGTGTCGACG+TGG 0.603292 7.1:+35388005 None:intergenic
GATGTCGAGGCCGATGATTG+CGG 0.603787 7.1:+35387964 None:intergenic
AAGCTGTTCGACCTTAGTGA+AGG 0.608000 7.1:+35387133 None:intergenic
AAGTGGGATTAAGAAATTAG+CGG 0.610373 7.1:-35387666 MS.gene94737:CDS
AGGGGAGGTAGTTGTCGATG+AGG 0.611336 7.1:+35388104 None:intergenic
AGGATCAATGAGGCTTGCTG+TGG 0.611605 7.1:-35386883 MS.gene94737:CDS
ACTAAGATGGTGATGCAGAA+GGG 0.612291 7.1:-35387065 MS.gene94737:CDS
GGGGAGGTAGTTGTCGATGA+GGG 0.612787 7.1:+35388105 None:intergenic
TGTGCAGAGCTGGAGAGTTG+CGG 0.615992 7.1:+35387901 None:intergenic
TTGGACGCCATTCGATGTCG+AGG 0.616614 7.1:+35387951 None:intergenic
TAACAAGTTCATATTCAACA+AGG 0.629475 7.1:+35386793 None:intergenic
TAAGGTGGTGAATACTAAGA+TGG 0.631699 7.1:-35387078 MS.gene94737:CDS
CCACACTCTGACTGTTAAGG+TGG 0.632768 7.1:-35387093 MS.gene94737:CDS
ACATGTTTAAAGGATCAATG+AGG 0.632902 7.1:-35386893 MS.gene94737:CDS
CCTCAAGCACCTCAGCAGCA+AGG 0.633154 7.1:+35387705 None:intergenic
AAGACGACGAGCAATCTCAA+AGG 0.634616 7.1:+35387529 None:intergenic
AACAAGTTCATATTCAACAA+GGG 0.636521 7.1:+35386794 None:intergenic
AGATTGTTGAGAAGCCAAAG+TGG 0.637456 7.1:-35387628 MS.gene94737:CDS
TCAGCAATTCGCATCTGACG+AGG 0.644396 7.1:+35387023 None:intergenic
CCACCTTAACAGTCAGAGTG+TGG 0.652542 7.1:+35387093 None:intergenic
TGAGATGGAAGATAAGGCAG+CGG 0.659112 7.1:+35387873 None:intergenic
GAGGAGCCAGGTGTCGACGT+GGG 0.661679 7.1:+35388006 None:intergenic
AGGTCGAACAGCTTCAACCG+GGG 0.663813 7.1:-35387124 MS.gene94737:CDS
GTTTCAGGTTAAGATTATGG+CGG 0.668082 7.1:-35387183 MS.gene94737:CDS
GTCGAGGCCGATGATTGCGG+GGG 0.672413 7.1:+35387967 None:intergenic
CAACATAATTCGCAACTGTG+AGG 0.681320 7.1:+35387738 None:intergenic
AGGCTTGCTGTGGACAAATG+GGG 0.694034 7.1:-35386873 MS.gene94737:CDS
AGAAGCCAAAGTGGATTACG+AGG 0.699220 7.1:-35387619 MS.gene94737:CDS
TAGGATCTGTTACTTCAACA+CGG 0.728623 7.1:+35386850 None:intergenic
TGGCGTCCAAACTCCCAACG+CGG 0.767636 7.1:-35387938 MS.gene94737:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTGTTATTAGAATCTT+AGG - chr7.1:35387570-35387589 MS.gene94737:CDS 15.0%
!! AGATTCTAATAACAAATTTC+TGG + chr7.1:35387569-35387588 None:intergenic 20.0%
!!! TTCAAGTAGTTATGATTTTA+AGG - chr7.1:35387530-35387549 MS.gene94737:CDS 20.0%
! AACAAGTTCATATTCAACAA+GGG + chr7.1:35388145-35388164 None:intergenic 25.0%
! GCAAAAATTGACATGTTTAA+AGG - chr7.1:35388033-35388052 MS.gene94737:CDS 25.0%
! TAACAAGTTCATATTCAACA+AGG + chr7.1:35388146-35388165 None:intergenic 25.0%
!!! ATATGAACTTGTTAATGTTG+TGG - chr7.1:35388152-35388171 MS.gene94737:CDS 25.0%
!!! ATTCCTCTAGTTTTATGTTA+AGG - chr7.1:35387470-35387489 MS.gene94737:intron 25.0%
AAGTGGGATTAAGAAATTAG+CGG - chr7.1:35387270-35387289 MS.gene94737:intron 30.0%
ACATGTTTAAAGGATCAATG+AGG - chr7.1:35388043-35388062 MS.gene94737:CDS 30.0%
GTATTCGATGATGATTATTC+TGG - chr7.1:35387506-35387525 MS.gene94737:intron 30.0%
TCACCTTAACATAAAACTAG+AGG + chr7.1:35387476-35387495 None:intergenic 30.0%
!! ATCGTGATTTGTTGAAAAGT+GGG - chr7.1:35387254-35387273 MS.gene94737:intron 30.0%
CCATAATCTTAACCTGAAAC+TGG + chr7.1:35387753-35387772 None:intergenic 35.0%
TAAGGTGGTGAATACTAAGA+TGG - chr7.1:35387858-35387877 MS.gene94737:CDS 35.0%
TCCTTAACAGTGAAATCAGT+AGG + chr7.1:35388108-35388127 None:intergenic 35.0%
TTCTATAAGCTTCTCGATGT+CGG + chr7.1:35387170-35387189 None:intergenic 35.0%
! GGATAATACTTGGCTTGATT+GGG - chr7.1:35387348-35387367 MS.gene94737:intron 35.0%
! TAGGATCTGTTACTTCAACA+CGG + chr7.1:35388089-35388108 None:intergenic 35.0%
!! CCAGTTTCAGGTTAAGATTA+TGG - chr7.1:35387750-35387769 MS.gene94737:CDS 35.0%
!! GATCGTGATTTGTTGAAAAG+TGG - chr7.1:35387253-35387272 MS.gene94737:intron 35.0%
!! GTTTCAGGTTAAGATTATGG+CGG - chr7.1:35387753-35387772 MS.gene94737:CDS 35.0%
!! TCCTACTGATTTCACTGTTA+AGG - chr7.1:35388104-35388123 MS.gene94737:CDS 35.0%
AAATTAGCGGAACGTGTTCT+TGG - chr7.1:35387283-35387302 MS.gene94737:intron 40.0%
AACCCTAACAACAGATTCGT+CGG - chr7.1:35387130-35387149 MS.gene94737:CDS 40.0%
AATTAGCGGAACGTGTTCTT+GGG - chr7.1:35387284-35387303 MS.gene94737:intron 40.0%
AATTGCTGAGAGTTTGGTTG+GGG - chr7.1:35387927-35387946 MS.gene94737:CDS 40.0%
AGATTGTTGAGAAGCCAAAG+TGG - chr7.1:35387308-35387327 MS.gene94737:intron 40.0%
AGTGGATGAGATGGAAGATA+AGG + chr7.1:35387072-35387091 None:intergenic 40.0%
ATCTGTTGTTAGGGTTAGAG+AGG + chr7.1:35387126-35387145 None:intergenic 40.0%
CAACATAATTCGCAACTGTG+AGG + chr7.1:35387201-35387220 None:intergenic 40.0%
GAATTGCTGAGAGTTTGGTT+GGG - chr7.1:35387926-35387945 MS.gene94737:CDS 40.0%
GGAGTAGATGGGATAATACT+TGG - chr7.1:35387338-35387357 MS.gene94737:intron 40.0%
GTTCATATTCAACAAGGGAC+AGG + chr7.1:35388140-35388159 None:intergenic 40.0%
! ACTAAGATGGTGATGCAGAA+GGG - chr7.1:35387871-35387890 MS.gene94737:CDS 40.0%
! GGGATAATACTTGGCTTGAT+TGG - chr7.1:35387347-35387366 MS.gene94737:intron 40.0%
! TACTAAGATGGTGATGCAGA+AGG - chr7.1:35387870-35387889 MS.gene94737:CDS 40.0%
! TTTTGGTCAACGAGGGTAAT+CGG + chr7.1:35386785-35386804 None:intergenic 40.0%
AAGACGACGAGCAATCTCAA+AGG + chr7.1:35387410-35387429 None:intergenic 45.0%
AAGCTGTTCGACCTTAGTGA+AGG + chr7.1:35387806-35387825 None:intergenic 45.0%
CAAGTCGATCTGCTGAAAGA+TGG - chr7.1:35387988-35388007 MS.gene94737:CDS 45.0%
CGAATTGCTGAGAGTTTGGT+TGG - chr7.1:35387925-35387944 MS.gene94737:CDS 45.0%
CTACTCCTCGTAATCCACTT+TGG + chr7.1:35387325-35387344 None:intergenic 45.0%
GATGCGAATTGCTGAGAGTT+TGG - chr7.1:35387921-35387940 MS.gene94737:CDS 45.0%
GCAGTTTCACTTCCAGTTTC+AGG - chr7.1:35387738-35387757 MS.gene94737:CDS 45.0%
GTTAGAGAGGAAGGTGAAGA+GGG + chr7.1:35387113-35387132 None:intergenic 45.0%
GTTGTTAGGGTTAGAGAGGA+AGG + chr7.1:35387122-35387141 None:intergenic 45.0%
TAAGGTCGAACAGCTTCAAC+CGG - chr7.1:35387810-35387829 MS.gene94737:CDS 45.0%
TCAGGTTAAGATTATGGCGG+AGG - chr7.1:35387756-35387775 MS.gene94737:CDS 45.0%
TCGATGAGGGTGATGTTGAA+GGG + chr7.1:35386821-35386840 None:intergenic 45.0%
TGAGATGGAAGATAAGGCAG+CGG + chr7.1:35387066-35387085 None:intergenic 45.0%
TGTTCGACCTTAGTGAAGGT+CGG + chr7.1:35387802-35387821 None:intergenic 45.0%
! GTGGATTACGAGGAGTAGAT+GGG - chr7.1:35387327-35387346 MS.gene94737:intron 45.0%
! TACTTGGCTTGATTGGGATC+AGG - chr7.1:35387354-35387373 MS.gene94737:intron 45.0%
!! AGAAGCCAAAGTGGATTACG+AGG - chr7.1:35387317-35387336 MS.gene94737:intron 45.0%
!! AGTGGATTACGAGGAGTAGA+TGG - chr7.1:35387326-35387345 MS.gene94737:intron 45.0%
!! TGTGGAGTTTTGGTCAACGA+GGG + chr7.1:35386792-35386811 None:intergenic 45.0%
AACAACAGATTCGTCGGCGT+CGG - chr7.1:35387136-35387155 MS.gene94737:CDS 50.0%
AAGGTCGAACAGCTTCAACC+GGG - chr7.1:35387811-35387830 MS.gene94737:CDS 50.0%
ACGCCGACGAATCTGTTGTT+AGG + chr7.1:35387136-35387155 None:intergenic 50.0%
AGGAAGGTGAAGAGGGAAGT+GGG + chr7.1:35387106-35387125 None:intergenic 50.0%
AGTTTGGTTGGGGATGAGAC+TGG - chr7.1:35387937-35387956 MS.gene94737:CDS 50.0%
CCACACTCTGACTGTTAAGG+TGG - chr7.1:35387843-35387862 MS.gene94737:CDS 50.0%
CCACCTTAACAGTCAGAGTG+TGG + chr7.1:35387846-35387865 None:intergenic 50.0%
CGCCGACGAATCTGTTGTTA+GGG + chr7.1:35387135-35387154 None:intergenic 50.0%
GAGAAAGCCGACCTTCACTA+AGG - chr7.1:35387792-35387811 MS.gene94737:CDS 50.0%
GGTTAGAGAGGAAGGTGAAG+AGG + chr7.1:35387114-35387133 None:intergenic 50.0%
GTCGATGAGGGTGATGTTGA+AGG + chr7.1:35386822-35386841 None:intergenic 50.0%
GTTTGGTTGGGGATGAGACT+GGG - chr7.1:35387938-35387957 MS.gene94737:CDS 50.0%
TCAGCAATTCGCATCTGACG+AGG + chr7.1:35387916-35387935 None:intergenic 50.0%
TCGTCGTCTTCACTCTCATC+AGG - chr7.1:35387420-35387439 MS.gene94737:intron 50.0%
TGGAAGGAGAGTGGATGAGA+TGG + chr7.1:35387081-35387100 None:intergenic 50.0%
TGGCCACACTCTGACTGTTA+AGG - chr7.1:35387840-35387859 MS.gene94737:CDS 50.0%
TTTGCGTAACCTTGCTGCTG+AGG - chr7.1:35387222-35387241 MS.gene94737:intron 50.0%
! AAGGTCGGCTTTCTCAATCC+AGG + chr7.1:35387787-35387806 None:intergenic 50.0%
! AGGGTGATGTTGAAGGGTGT+TGG + chr7.1:35386815-35386834 None:intergenic 50.0%
! ATTATGGCGGAGGCAAAACC+TGG - chr7.1:35387766-35387785 MS.gene94737:CDS 50.0%
! TAGGTGTTGTGGGTGTCGTA+GGG + chr7.1:35386854-35386873 None:intergenic 50.0%
!! AGGATCAATGAGGCTTGCTG+TGG - chr7.1:35388053-35388072 MS.gene94737:CDS 50.0%
!! AGGCTTGCTGTGGACAAATG+GGG - chr7.1:35388063-35388082 MS.gene94737:CDS 50.0%
!! GAGGCTTGCTGTGGACAAAT+GGG - chr7.1:35388062-35388081 MS.gene94737:CDS 50.0%
!! GATGTTGAAGGGTGTTGGTG+TGG + chr7.1:35386810-35386829 None:intergenic 50.0%
!! GGTGATGGAGTAGGTGTTGT+GGG + chr7.1:35386864-35386883 None:intergenic 50.0%
!! GTGTGGAGTTTTGGTCAACG+AGG + chr7.1:35386793-35386812 None:intergenic 50.0%
!! TGAGGCTTGCTGTGGACAAA+TGG - chr7.1:35388061-35388080 MS.gene94737:CDS 50.0%
!! TGGTGATGGAGTAGGTGTTG+TGG + chr7.1:35386865-35386884 None:intergenic 50.0%
!!! AGGGTGTTGGTGTGGAGTTT+TGG + chr7.1:35386802-35386821 None:intergenic 50.0%
AAGAGGGAAGTGGGGATGGA+AGG + chr7.1:35387097-35387116 None:intergenic 55.0%
AAGTGGGGATGGAAGGAGAG+TGG + chr7.1:35387090-35387109 None:intergenic 55.0%
ACAGGTGAGGAGGGTTTGGA+TGG + chr7.1:35386903-35386922 None:intergenic 55.0%
AGCGGTTGTTTGTGCAGAGC+TGG + chr7.1:35387048-35387067 None:intergenic 55.0%
AGGGGAGGTAGTTGTCGATG+AGG + chr7.1:35386835-35386854 None:intergenic 55.0%
AGGTCGAACAGCTTCAACCG+GGG - chr7.1:35387812-35387831 MS.gene94737:CDS 55.0%
ATGCAGAAGGGTCGTCCCAA+TGG - chr7.1:35387883-35387902 MS.gene94737:CDS 55.0%
ATGTCGAGGCCGATGATTGC+GGG + chr7.1:35386974-35386993 None:intergenic 55.0%
ATTCGCATCTGACGAGGCTG+AGG + chr7.1:35387910-35387929 None:intergenic 55.0%
GAGGAAGGTGAAGAGGGAAG+TGG + chr7.1:35387107-35387126 None:intergenic 55.0%
GAGGAGGGTTTGGATGGTGA+AGG + chr7.1:35386897-35386916 None:intergenic 55.0%
GAGGGTTTGGATGGTGAAGG+TGG + chr7.1:35386894-35386913 None:intergenic 55.0%
GATGTCGAGGCCGATGATTG+CGG + chr7.1:35386975-35386994 None:intergenic 55.0%
GGAAGGTGAAGAGGGAAGTG+GGG + chr7.1:35387105-35387124 None:intergenic 55.0%
GGATTGAGTTTCGAGGAGCC+AGG + chr7.1:35386945-35386964 None:intergenic 55.0%
GGGAGAGGGATTGAGTTTCG+AGG + chr7.1:35386952-35386971 None:intergenic 55.0%
GGGGAGGTAGTTGTCGATGA+GGG + chr7.1:35386834-35386853 None:intergenic 55.0%
TCATCGGCCTCGACATCGAA+TGG - chr7.1:35386978-35386997 MS.gene94737:CDS 55.0%
TGACGAGGCTGAGGACCATT+GGG + chr7.1:35387901-35387920 None:intergenic 55.0%
TGTGCAGAGCTGGAGAGTTG+CGG + chr7.1:35387038-35387057 None:intergenic 55.0%
TTGGACGCCATTCGATGTCG+AGG + chr7.1:35386988-35387007 None:intergenic 55.0%
! AGGTGTTGTGGGTGTCGTAG+GGG + chr7.1:35386853-35386872 None:intergenic 55.0%
! GATGGTGAAGGTGGTGTCGA+TGG + chr7.1:35386885-35386904 None:intergenic 55.0%
! GTAGGTGTTGTGGGTGTCGT+AGG + chr7.1:35386855-35386874 None:intergenic 55.0%
!! GAAGGTGGTGTCGATGGTGA+TGG + chr7.1:35386879-35386898 None:intergenic 55.0%
!! GGTGTCGATGGTGATGGAGT+AGG + chr7.1:35386873-35386892 None:intergenic 55.0%
AGAGCTGGAGAGTTGCGGCT+GGG + chr7.1:35387033-35387052 None:intergenic 60.0%
AGAGTGTGGCCACTAGTCCC+CGG + chr7.1:35387832-35387851 None:intergenic 60.0%
CAGCTTCAACCGGGGACTAG+TGG - chr7.1:35387820-35387839 MS.gene94737:CDS 60.0%
CCTCAAGCACCTCAGCAGCA+AGG + chr7.1:35387234-35387253 None:intergenic 60.0%
CTGACGAGGCTGAGGACCAT+TGG + chr7.1:35387902-35387921 None:intergenic 60.0%
GAGTTGCGGCTGGGTTATCC+TGG + chr7.1:35387024-35387043 None:intergenic 60.0%
GGGAACAGGTGAGGAGGGTT+TGG + chr7.1:35386907-35386926 None:intergenic 60.0%
GGTGAAGAGGGAAGTGGGGA+TGG + chr7.1:35387101-35387120 None:intergenic 60.0%
TCCCTCTCCCCCGCAATCAT+CGG - chr7.1:35386962-35386981 MS.gene94737:CDS 60.0%
TGGCGTCCAAACTCCCAACG+CGG - chr7.1:35386998-35387017 MS.gene94737:CDS 60.0%
TGGGTTATCCTGGCCGCGTT+GGG + chr7.1:35387014-35387033 None:intergenic 60.0%
TGTCGAGGCCGATGATTGCG+GGG + chr7.1:35386973-35386992 None:intergenic 60.0%
! CCTTGCTGCTGAGGTGCTTG+AGG - chr7.1:35387231-35387250 MS.gene94737:intron 60.0%
! TGTTGTGGGTGTCGTAGGGG+AGG + chr7.1:35386850-35386869 None:intergenic 60.0%
CAGAGCTGGAGAGTTGCGGC+TGG + chr7.1:35387034-35387053 None:intergenic 65.0%
CTGGGTTATCCTGGCCGCGT+TGG + chr7.1:35387015-35387034 None:intergenic 65.0%
GCCGATGATTGCGGGGGAGA+GGG + chr7.1:35386966-35386985 None:intergenic 65.0%
GGTGGGGGAACAGGTGAGGA+GGG + chr7.1:35386912-35386931 None:intergenic 65.0%
GTCGAGGCCGATGATTGCGG+GGG + chr7.1:35386972-35386991 None:intergenic 65.0%
TCCAAACTCCCAACGCGGCC+AGG - chr7.1:35387003-35387022 MS.gene94737:CDS 65.0%
TCCTGGCCGCGTTGGGAGTT+TGG + chr7.1:35387007-35387026 None:intergenic 65.0%
!! AGCCAGGTGTCGACGTGGGT+GGG + chr7.1:35386929-35386948 None:intergenic 65.0%
!! GAGGAGCCAGGTGTCGACGT+GGG + chr7.1:35386933-35386952 None:intergenic 65.0%
CGTGGGTGGGGGAACAGGTG+AGG + chr7.1:35386916-35386935 None:intergenic 70.0%
GGCCGATGATTGCGGGGGAG+AGG + chr7.1:35386967-35386986 None:intergenic 70.0%
GGGTGGGGGAACAGGTGAGG+AGG + chr7.1:35386913-35386932 None:intergenic 70.0%
GTCGACGTGGGTGGGGGAAC+AGG + chr7.1:35386921-35386940 None:intergenic 70.0%
!! CCAGGTGTCGACGTGGGTGG+GGG + chr7.1:35386927-35386946 None:intergenic 70.0%
!! CGAGGAGCCAGGTGTCGACG+TGG + chr7.1:35386934-35386953 None:intergenic 70.0%
!! GAGCCAGGTGTCGACGTGGG+TGG + chr7.1:35386930-35386949 None:intergenic 70.0%
!! GCCAGGTGTCGACGTGGGTG+GGG + chr7.1:35386928-35386947 None:intergenic 70.0%
CCCCCACCCACGTCGACACC+TGG - chr7.1:35386924-35386943 MS.gene94737:CDS 75.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 35386779 35388179 35386779 ID=MS.gene94737
chr7.1 mRNA 35386779 35388179 35386779 ID=MS.gene94737.t1;Parent=MS.gene94737
chr7.1 exon 35387517 35388179 35387517 ID=MS.gene94737.t1.exon1;Parent=MS.gene94737.t1
chr7.1 CDS 35387517 35388179 35387517 ID=cds.MS.gene94737.t1;Parent=MS.gene94737.t1
chr7.1 exon 35386779 35387216 35386779 ID=MS.gene94737.t1.exon2;Parent=MS.gene94737.t1
chr7.1 CDS 35386779 35387216 35386779 ID=cds.MS.gene94737.t1;Parent=MS.gene94737.t1
Gene Sequence

>MS.gene94737

ATGCCGATTACCCTCGTTGACCAAAACTCCACACCAACACCCTTCAACATCACCCTCATCGACAACTACCTCCCCTACGACACCCACAACACCTACTCCATCACCATCGACACCACCTTCACCATCCAAACCCTCCTCACCTGTTCCCCCACCCACGTCGACACCTGGCTCCTCGAAACTCAATCCCTCTCCCCCGCAATCATCGGCCTCGACATCGAATGGCGTCCAAACTCCCAACGCGGCCAGGATAACCCAGCCGCAACTCTCCAGCTCTGCACAAACAACCGCTGCCTTATCTTCCATCTCATCCACTCTCCTTCCATCCCCACTTCCCTCTTCACCTTCCTCTCTAACCCTAACAACAGATTCGTCGGCGTCGGAATCGAATCCGACATCGAGAAGCTTATAGAAGACTACAACCTCACAGTTGCGAATTATGTTGATTTGCGTAACCTTGCTGCTGAGGTGCTTGAGGATCGTGATTTGTTGAAAAGTGGGATTAAGAAATTAGCGGAACGTGTTCTTGGGAAGATTGTTGAGAAGCCAAAGTGGATTACGAGGAGTAGATGGGATAATACTTGGCTTGATTGGGATCAGGTTCAGTATGCTACAGTTGATGCTTTTCTTTCCTTTGAGATTGCTCGTCGTCTTCACTCTCATCAGGTTATTGAATGAATGAACATGATTGATGATTCCTCTAGTTTTATGTTAAGGTGATTGTGATGATGTATTCGATGATGATTATTCTGGATTCAAGTAGTTATGATTTTAAGGTTTATCATTTGTGCCAGAAATTTGTTATTAGAATCTTAGGTGTGTATTTTATCGTTTGATTCTGCGTTGACGAAAGTTTATTTTGTTAAATTGATTTTATTAAAAGAAATAAACAATAAATTTCAGTACAAAATCAGTTTAGAAACAAAAGCTAGAATCTATATTGATAGCAAAATCAACTATAAGCAGTTTCACTTCCAGTTTCAGGTTAAGATTATGGCGGAGGCAAAACCTGGATTGAGAAAGCCGACCTTCACTAAGGTCGAACAGCTTCAACCGGGGACTAGTGGCCACACTCTGACTGTTAAGGTGGTGAATACTAAGATGGTGATGCAGAAGGGTCGTCCCAATGGTCCTCAGCCTCGTCAGATGCGAATTGCTGAGAGTTTGGTTGGGGATGAGACTGGGATGATCATCTTTACTGCAAGAAATGATCAAGTCGATCTGCTGAAAGATGGCTCTACTATCATTTTGCGAAATGCAAAAATTGACATGTTTAAAGGATCAATGAGGCTTGCTGTGGACAAATGGGGCCGTGTTGAAGTAACAGATCCTACTGATTTCACTGTTAAGGAAGACAACAACCTGTCCCTTGTTGAATATGAACTTGTTAATGTTGTGGCGTAA
Protein sequence

>MS.gene94737.t1

MPITLVDQNSTPTPFNITLIDNYLPYDTHNTYSITIDTTFTIQTLLTCSPTHVDTWLLETQSLSPAIIGLDIEWRPNSQRGQDNPAATLQLCTNNRCLIFHLIHSPSIPTSLFTFLSNPNNRFVGVGIESDIEKLIEDYNLTVANYVDLRNLAAEVLEDRDLLKSGIKKLAERVLGKIVEKPKWITRSRWDNTWLDWDQVQYATVDAFLSFEIARRLHSHQFHFQFQVKIMAEAKPGLRKPTFTKVEQLQPGTSGHTLTVKVVNTKMVMQKGRPNGPQPRQMRIAESLVGDETGMIIFTARNDQVDLLKDGSTIILRNAKIDMFKGSMRLAVDKWGRVEVTDPTDFTVKEDNNLSLVEYELVNVVA