Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94862.t1 | XP_004516700.1 | 90 | 399 | 37 | 1 | 1 | 396 | 132 | 530 | 3.00E-206 | 727.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94862.t1 | A0A1S2Z812 | 90.0 | 399 | 37 | 1 | 1 | 396 | 132 | 530 | 2.2e-206 | 727.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene94862 | 0.803533 | 3.33E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene94862 | 0.816569 | 5.17E-52 | -1.69E-46 |
| MS.gene055539 | MS.gene94862 | 0.835243 | 1.88E-56 | -1.69E-46 |
| MS.gene058120 | MS.gene94862 | 0.821042 | 4.98E-53 | -1.69E-46 |
| MS.gene058948 | MS.gene94862 | 0.831872 | 1.31E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94862.t1 | MTR_6g053110 | 92.929 | 396 | 3 | 2 | 1 | 396 | 137 | 507 | 0.0 | 744 |
| MS.gene94862.t1 | MTR_6g053110 | 97.034 | 236 | 7 | 0 | 1 | 236 | 137 | 372 | 5.83e-170 | 480 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94862.t1 | AT5G62650 | 64.516 | 403 | 132 | 6 | 1 | 396 | 130 | 528 | 0.0 | 516 |
Find 86 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAGCATCATCTTCCAATTT+AGG | 0.100505 | 6.1:-29506357 | None:intergenic |
| ACCAAAAGGGAGTCTTAATT+TGG | 0.190178 | 6.1:-29506451 | None:intergenic |
| GAGGAAATTGGAGATAGTTT+AGG | 0.227554 | 6.1:+29500951 | MS.gene94862:CDS |
| TCAAACTAGCTTTGCTCATT+TGG | 0.235071 | 6.1:-29506614 | None:intergenic |
| AGGAAATTGGAGATAGTTTA+GGG | 0.236987 | 6.1:+29500952 | MS.gene94862:CDS |
| ACAATCCCACACAGTGGTTT+TGG | 0.240085 | 6.1:+29500070 | MS.gene94862:CDS |
| ATCAAGCAGCTGCCCTAAAT+TGG | 0.252779 | 6.1:+29506344 | MS.gene94862:CDS |
| ACAACCGAAATTTCCATATA+TGG | 0.268063 | 6.1:+29504619 | MS.gene94862:CDS |
| TCAAATGAGTCAACCATATA+TGG | 0.275386 | 6.1:-29504632 | None:intergenic |
| GCTTCTCGTTGGGAACAAAT+TGG | 0.281103 | 6.1:-29499936 | None:intergenic |
| GCCGAAGTGGTTGAGGAAAT+TGG | 0.282944 | 6.1:+29500939 | MS.gene94862:CDS |
| TAGCATCATCTTCCAATTTA+GGG | 0.287330 | 6.1:-29506356 | None:intergenic |
| TAATTTGGAATGATCGTCTT+TGG | 0.306511 | 6.1:-29506436 | None:intergenic |
| AGGGCCATTCCTCTCACTTT+TGG | 0.310613 | 6.1:-29498748 | None:intergenic |
| TCAACCATATATGGAAATTT+CGG | 0.312417 | 6.1:-29504623 | None:intergenic |
| GCCGCTGAATGTGCCGAGTT+TGG | 0.318648 | 6.1:-29500887 | None:intergenic |
| ACAAAGTACTCTTGGGTAAT+AGG | 0.319800 | 6.1:+29504573 | MS.gene94862:CDS |
| CAACTACAGATGGTATTAAA+TGG | 0.353512 | 6.1:+29500149 | MS.gene94862:CDS |
| TCAAGAGGTGCTTGATGAAA+TGG | 0.359395 | 6.1:+29504484 | MS.gene94862:CDS |
| TGTCAACAGTATAATGAAAC+TGG | 0.388539 | 6.1:-29504553 | None:intergenic |
| TACTGTTGACAAAGTACTCT+TGG | 0.392001 | 6.1:+29504565 | MS.gene94862:CDS |
| CCCACATTAGCTACGAATAG+TGG | 0.399716 | 6.1:-29506735 | None:intergenic |
| AATTATACCTGCTTCTCGTT+GGG | 0.400296 | 6.1:-29499946 | None:intergenic |
| TCCAAATTAAGACTCCCTTT+TGG | 0.403564 | 6.1:+29506450 | MS.gene94862:CDS |
| TTTAGCTTATGAACATTTCA+AGG | 0.411237 | 6.1:+29499701 | MS.gene94862:CDS |
| TTCAGAAAAGTCTGTACAAC+CGG | 0.413910 | 6.1:-29506385 | None:intergenic |
| CGTTGGGAACAAATTGGAAC+AGG | 0.435543 | 6.1:-29499930 | None:intergenic |
| GAAGATGATGCTAAAGAAAC+CGG | 0.458835 | 6.1:+29506366 | MS.gene94862:CDS |
| GGATGATTTGACAAAAGAGT+TGG | 0.465210 | 6.1:+29506647 | MS.gene94862:CDS |
| CCTCAACCACTTCGGCTGCC+AGG | 0.465561 | 6.1:-29500932 | None:intergenic |
| TTTATTGATCTAGGAGATGC+AGG | 0.471377 | 6.1:+29498783 | MS.gene94862:CDS |
| TGAGTACAAATTAGAAGATC+GGG | 0.471795 | 6.1:+29506710 | MS.gene94862:CDS |
| TGGTTGACTCATTTGAAAGA+AGG | 0.477306 | 6.1:+29504639 | MS.gene94862:CDS |
| CAGGCACCCACCACGCCACT+TGG | 0.483053 | 6.1:-29499669 | None:intergenic |
| ACTGTTGACAAAGTACTCTT+GGG | 0.486851 | 6.1:+29504566 | MS.gene94862:CDS |
| TCATGAAACCAATCACCAAA+AGG | 0.488144 | 6.1:-29506465 | None:intergenic |
| CCTGGCAGCCGAAGTGGTTG+AGG | 0.489290 | 6.1:+29500932 | MS.gene94862:CDS |
| CAAGAGGTGCTTGATGAAAT+GGG | 0.490240 | 6.1:+29504485 | MS.gene94862:CDS |
| AAATTATACCTGCTTCTCGT+TGG | 0.491815 | 6.1:-29499947 | None:intergenic |
| AAAGCAGGTTTATCAAGAAA+AGG | 0.495281 | 6.1:+29506513 | MS.gene94862:CDS |
| ATGAGTACAAATTAGAAGAT+CGG | 0.495909 | 6.1:+29506709 | MS.gene94862:CDS |
| GGAAGTCCTCGTTGGATTCG+AGG | 0.502433 | 6.1:+29506786 | MS.gene94862:CDS |
| TGTGTTTAGTGCTCACAACT+TGG | 0.502465 | 6.1:+29500114 | MS.gene94862:CDS |
| TATTCTAGTGTTGGAAGGAC+GGG | 0.503511 | 6.1:+29506765 | MS.gene94862:CDS |
| ATTTGTTCCCAACGAGAAGC+AGG | 0.503660 | 6.1:+29499939 | MS.gene94862:CDS |
| TCCAATTTCCTCAACCACTT+CGG | 0.504874 | 6.1:-29500940 | None:intergenic |
| AGGTGGTTGCGGAAAGCGAC+AGG | 0.506989 | 6.1:+29504593 | MS.gene94862:CDS |
| GCCGAGTTTGGATTAGTGTA+TGG | 0.510363 | 6.1:-29500875 | None:intergenic |
| GCAGACAATGCTACTGTTGG+TGG | 0.521383 | 6.1:+29506681 | MS.gene94862:CDS |
| CCCACTATTCGTAGCTAATG+TGG | 0.523741 | 6.1:+29506734 | MS.gene94862:CDS |
| CATTCAGCGGCGGCGAGATG+TGG | 0.531124 | 6.1:+29500899 | MS.gene94862:CDS |
| GGTGATTATTATTCTAGTGT+TGG | 0.533354 | 6.1:+29506756 | MS.gene94862:CDS |
| ATTATTATTCTAGTGTTGGA+AGG | 0.534001 | 6.1:+29506760 | MS.gene94862:CDS |
| TTATTCTAGTGTTGGAAGGA+CGG | 0.554799 | 6.1:+29506764 | MS.gene94862:CDS |
| CATGAAACCAATCACCAAAA+GGG | 0.565941 | 6.1:-29506464 | None:intergenic |
| TCCAAACTCGGCACATTCAG+CGG | 0.567084 | 6.1:+29500886 | MS.gene94862:CDS |
| AATATTCCAAGTGGCGTGGT+GGG | 0.574038 | 6.1:+29499663 | MS.gene94862:CDS |
| AAATATTCCAAGTGGCGTGG+TGG | 0.589010 | 6.1:+29499662 | MS.gene94862:CDS |
| GAAGGACGGGAAGTCCTCGT+TGG | 0.593326 | 6.1:+29506778 | MS.gene94862:CDS |
| GGTCTCTCTACTGAAGAAGC+AGG | 0.594013 | 6.1:+29506591 | MS.gene94862:CDS |
| ATAAGCTAAATCGAGACGGC+AGG | 0.596438 | 6.1:-29499688 | None:intergenic |
| CACTGAGTGGTATACTCGGA+TGG | 0.601860 | 6.1:-29504508 | None:intergenic |
| TAAACTAGTCATACCTCTGG+AGG | 0.603392 | 6.1:-29500978 | None:intergenic |
| CCACTATTCGTAGCTAATGT+GGG | 0.605324 | 6.1:+29506735 | MS.gene94862:CDS |
| ACTAAATATTCCAAGTGGCG+TGG | 0.609725 | 6.1:+29499659 | MS.gene94862:CDS |
| TCTTGGGTAATAGGTGGTTG+CGG | 0.610993 | 6.1:+29504582 | MS.gene94862:CDS |
| GGAAATTGGAGATAGTTTAG+GGG | 0.611002 | 6.1:+29500953 | MS.gene94862:CDS |
| GAAGCAGACAATGCTACTGT+TGG | 0.611764 | 6.1:+29506678 | MS.gene94862:CDS |
| CCACATTAGCTACGAATAGT+GGG | 0.612532 | 6.1:-29506734 | None:intergenic |
| TCCATACACTAATCCAAACT+CGG | 0.614245 | 6.1:+29500874 | MS.gene94862:CDS |
| TAGTTTAGGGGAGCCTCCAG+AGG | 0.617502 | 6.1:+29500965 | MS.gene94862:CDS |
| ATTGCAATAGCAACTACAGA+TGG | 0.619655 | 6.1:+29500139 | MS.gene94862:CDS |
| AGATGTGGTTGATGATAACC+TGG | 0.623716 | 6.1:+29500914 | MS.gene94862:CDS |
| ATAACACTGAGTGGTATACT+CGG | 0.624599 | 6.1:-29504512 | None:intergenic |
| GGAACAAATTGGAACAGGTG+AGG | 0.626637 | 6.1:-29499925 | None:intergenic |
| CAGCAATCACCAAAAGTGAG+AGG | 0.633326 | 6.1:+29498739 | MS.gene94862:CDS |
| ATCACCAAAAGTGAGAGGAA+TGG | 0.635439 | 6.1:+29498744 | MS.gene94862:CDS |
| GTTCATAAGCTAAATCGAGA+CGG | 0.645085 | 6.1:-29499692 | None:intergenic |
| TGATAACCTGGCAGCCGAAG+TGG | 0.657256 | 6.1:+29500926 | MS.gene94862:CDS |
| AAGTACTCTTGGGTAATAGG+TGG | 0.663674 | 6.1:+29504576 | MS.gene94862:CDS |
| AAACTCGGCACATTCAGCGG+CGG | 0.687036 | 6.1:+29500889 | MS.gene94862:CDS |
| GCTTTAGAAATAACACTGAG+TGG | 0.689454 | 6.1:-29504521 | None:intergenic |
| AAGTTGTGAGCACTAAACAC+AGG | 0.711561 | 6.1:-29500112 | None:intergenic |
| AGTCCTCGTTGGATTCGAGG+AGG | 0.715571 | 6.1:+29506789 | MS.gene94862:CDS |
| GATCCTCCTCGAATCCAACG+AGG | 0.730797 | 6.1:-29506792 | None:intergenic |
| CTGAAGACAATCCCACACAG+TGG | 0.749428 | 6.1:+29500064 | MS.gene94862:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 29498732 | 29506821 | 29498732 | ID=MS.gene94862 |
| chr6.1 | mRNA | 29498732 | 29506821 | 29498732 | ID=MS.gene94862.t1;Parent=MS.gene94862 |
| chr6.1 | exon | 29498732 | 29498804 | 29498732 | ID=MS.gene94862.t1.exon1;Parent=MS.gene94862.t1 |
| chr6.1 | CDS | 29498732 | 29498804 | 29498732 | ID=cds.MS.gene94862.t1;Parent=MS.gene94862.t1 |
| chr6.1 | exon | 29499643 | 29499722 | 29499643 | ID=MS.gene94862.t1.exon2;Parent=MS.gene94862.t1 |
| chr6.1 | CDS | 29499643 | 29499722 | 29499643 | ID=cds.MS.gene94862.t1;Parent=MS.gene94862.t1 |
| chr6.1 | exon | 29499919 | 29499960 | 29499919 | ID=MS.gene94862.t1.exon3;Parent=MS.gene94862.t1 |
| chr6.1 | CDS | 29499919 | 29499960 | 29499919 | ID=cds.MS.gene94862.t1;Parent=MS.gene94862.t1 |
| chr6.1 | exon | 29500043 | 29500170 | 29500043 | ID=MS.gene94862.t1.exon4;Parent=MS.gene94862.t1 |
| chr6.1 | CDS | 29500043 | 29500170 | 29500043 | ID=cds.MS.gene94862.t1;Parent=MS.gene94862.t1 |
| chr6.1 | exon | 29500806 | 29500986 | 29500806 | ID=MS.gene94862.t1.exon5;Parent=MS.gene94862.t1 |
| chr6.1 | CDS | 29500806 | 29500986 | 29500806 | ID=cds.MS.gene94862.t1;Parent=MS.gene94862.t1 |
| chr6.1 | exon | 29504482 | 29504660 | 29504482 | ID=MS.gene94862.t1.exon6;Parent=MS.gene94862.t1 |
| chr6.1 | CDS | 29504482 | 29504660 | 29504482 | ID=cds.MS.gene94862.t1;Parent=MS.gene94862.t1 |
| chr6.1 | exon | 29506314 | 29506821 | 29506314 | ID=MS.gene94862.t1.exon7;Parent=MS.gene94862.t1 |
| chr6.1 | CDS | 29506314 | 29506821 | 29506314 | ID=cds.MS.gene94862.t1;Parent=MS.gene94862.t1 |
| Gene Sequence |
| Protein sequence |
