Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95055.t1 | XP_024637289.1 | 86.6 | 67 | 9 | 0 | 4 | 70 | 3 | 69 | 2.30E-24 | 120.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95055.t1 | Q9M9R9 | 76.6 | 47 | 11 | 0 | 8 | 54 | 8 | 54 | 2.8e-15 | 82.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95055.t1 | I3S7T5 | 86.6 | 67 | 9 | 0 | 4 | 70 | 3 | 69 | 1.7e-24 | 120.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene061145 | MS.gene95055 | 0.801452 | 8.95E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95055.t1 | MTR_4g134550 | 92.308 | 65 | 5 | 0 | 4 | 68 | 3 | 67 | 4.04e-34 | 111 |
| MS.gene95055.t1 | MTR_2g036340 | 60.345 | 58 | 22 | 1 | 8 | 64 | 3 | 60 | 1.70e-18 | 72.0 |
| MS.gene95055.t1 | MTR_8g046930 | 60.345 | 58 | 22 | 1 | 8 | 64 | 3 | 60 | 1.70e-18 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95055.t1 | AT1G14450 | 71.186 | 59 | 16 | 1 | 7 | 64 | 7 | 65 | 2.03e-26 | 92.0 |
| MS.gene95055.t1 | AT1G14450 | 71.186 | 59 | 16 | 1 | 7 | 64 | 31 | 89 | 2.93e-26 | 92.4 |
| MS.gene95055.t1 | AT2G02510 | 62.121 | 66 | 23 | 1 | 7 | 70 | 7 | 72 | 1.84e-24 | 87.0 |
Find 20 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCATCGCTCTTGTCGCTTT+TGG | 0.259915 | 8.2:-306409 | MS.gene95055:CDS |
| GGAAGAGGAATTCATCTTGT+TGG | 0.397407 | 8.2:+306360 | None:intergenic |
| TACTTTCGCAGGTGAAGGCT+GGG | 0.420519 | 8.2:-306008 | MS.gene95055:intron |
| TTACTTTCGCAGGTGAAGGC+TGG | 0.453299 | 8.2:-306009 | MS.gene95055:intron |
| TCTTCAAGAGGAGGGATGAA+TGG | 0.473314 | 8.2:-306483 | MS.gene95055:CDS |
| CGTCATGCCACTCCTGGTCT+CGG | 0.476105 | 8.2:-306430 | MS.gene95055:CDS |
| ATGGTGATGAGAAGAGGAAG+AGG | 0.488621 | 8.2:+306345 | None:intergenic |
| CAGATGCGTCATGCCACTCC+TGG | 0.493267 | 8.2:-306436 | MS.gene95055:CDS |
| ACAAGAGCGATGCCGAGACC+AGG | 0.501831 | 8.2:+306418 | None:intergenic |
| TCGGAAGAAATGGGAGCAGC+AGG | 0.508971 | 8.2:-306523 | None:intergenic |
| TGGAGGAAGCATCCCATGCT+TGG | 0.534788 | 8.2:-306463 | MS.gene95055:CDS |
| CGCATCTGATTACCAAGCAT+GGG | 0.552200 | 8.2:+306451 | None:intergenic |
| AGATACATGGTGATGAGAAG+AGG | 0.553982 | 8.2:+306339 | None:intergenic |
| ACGCATCTGATTACCAAGCA+TGG | 0.557487 | 8.2:+306450 | None:intergenic |
| AGCAGAGTTCTTCAAGAGGA+GGG | 0.574966 | 8.2:-306491 | MS.gene95055:CDS |
| CAGCAGAGTTCTTCAAGAGG+AGG | 0.588158 | 8.2:-306492 | MS.gene95055:CDS |
| ACTTTCGCAGGTGAAGGCTG+GGG | 0.593895 | 8.2:-306007 | MS.gene95055:intron |
| AGCGATGCCGAGACCAGGAG+TGG | 0.620806 | 8.2:+306423 | None:intergenic |
| TCAAGAGGAGGGATGAATGG+AGG | 0.649135 | 8.2:-306480 | MS.gene95055:CDS |
| ATACAGCAGAGTTCTTCAAG+AGG | 0.667371 | 8.2:-306495 | MS.gene95055:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGCATTGTTATAATTTAAT+GGG | + | chr8.2:306428-306447 | None:intergenic | 15.0% |
| !! | AATAAAATGTAAATTGGGAA+TGG | + | chr8.2:306265-306284 | None:intergenic | 20.0% |
| !! | AGCAGTAATAAAATGTAAAT+TGG | + | chr8.2:306271-306290 | None:intergenic | 20.0% |
| !! | ATAAAATGTAAATTGGGAAT+GGG | + | chr8.2:306264-306283 | None:intergenic | 20.0% |
| !! | ATGTATCTATTCTTATTCTT+AGG | - | chr8.2:306189-306208 | MS.gene95055:intron | 20.0% |
| !! | GCAGTAATAAAATGTAAATT+GGG | + | chr8.2:306270-306289 | None:intergenic | 20.0% |
| !! | TAAGAATAAGAATAGATACA+TGG | + | chr8.2:306190-306209 | None:intergenic | 20.0% |
| !! | TGTATCTATTCTTATTCTTA+GGG | - | chr8.2:306190-306209 | MS.gene95055:intron | 20.0% |
| !!! | ATTTACATTTTATTACTGCT+AGG | - | chr8.2:306271-306290 | MS.gene95055:intron | 20.0% |
| !!! | GAAGCATTGTTATAATTTAA+TGG | + | chr8.2:306429-306448 | None:intergenic | 20.0% |
| !!! | TTTACATTTTATTACTGCTA+GGG | - | chr8.2:306272-306291 | MS.gene95055:intron | 20.0% |
| ! | TGTTTAGAAAGAATAGAGAT+TGG | - | chr8.2:306368-306387 | MS.gene95055:CDS | 25.0% |
| CTTAATTACATTACTTTCGC+AGG | - | chr8.2:306494-306513 | MS.gene95055:CDS | 30.0% | |
| AAATGTAAATTGGGAATGGG+AGG | + | chr8.2:306261-306280 | None:intergenic | 35.0% | |
| AATGTAAATTGGGAATGGGA+GGG | + | chr8.2:306260-306279 | None:intergenic | 35.0% | |
| TGAATAGTACAAACAGACAC+AGG | + | chr8.2:306468-306487 | None:intergenic | 35.0% | |
| TTCATTATGAAAACGAGTCC+CGG | + | chr8.2:306399-306418 | None:intergenic | 35.0% | |
| ATACAGCAGAGTTCTTCAAG+AGG | - | chr8.2:306018-306037 | MS.gene95055:intron | 40.0% | |
| GGAAGAGGAATTCATCTTGT+TGG | + | chr8.2:306156-306175 | None:intergenic | 40.0% | |
| TACATTACTTTCGCAGGTGA+AGG | - | chr8.2:306500-306519 | MS.gene95055:CDS | 40.0% | |
| ! | AGAATAGAGATTGGTTGCAC+CGG | - | chr8.2:306377-306396 | MS.gene95055:CDS | 40.0% |
| ! | AGATACATGGTGATGAGAAG+AGG | + | chr8.2:306177-306196 | None:intergenic | 40.0% |
| ACGCATCTGATTACCAAGCA+TGG | + | chr8.2:306066-306085 | None:intergenic | 45.0% | |
| AGCAGAGTTCTTCAAGAGGA+GGG | - | chr8.2:306022-306041 | MS.gene95055:intron | 45.0% | |
| CATACAACAAGCAACGATGC+TGG | + | chr8.2:306329-306348 | None:intergenic | 45.0% | |
| CGCATCTGATTACCAAGCAT+GGG | + | chr8.2:306065-306084 | None:intergenic | 45.0% | |
| TCTTCAAGAGGAGGGATGAA+TGG | - | chr8.2:306030-306049 | MS.gene95055:intron | 45.0% | |
| ! | ATGGTGATGAGAAGAGGAAG+AGG | + | chr8.2:306171-306190 | None:intergenic | 45.0% |
| ! | GAATAGAGATTGGTTGCACC+GGG | - | chr8.2:306378-306397 | MS.gene95055:CDS | 45.0% |
| CAGCAGAGTTCTTCAAGAGG+AGG | - | chr8.2:306021-306040 | MS.gene95055:intron | 50.0% | |
| TACTTTCGCAGGTGAAGGCT+GGG | - | chr8.2:306505-306524 | MS.gene95055:CDS | 50.0% | |
| TCAAGAGGAGGGATGAATGG+AGG | - | chr8.2:306033-306052 | MS.gene95055:intron | 50.0% | |
| TTACTTTCGCAGGTGAAGGC+TGG | - | chr8.2:306504-306523 | MS.gene95055:CDS | 50.0% | |
| ACTTTCGCAGGTGAAGGCTG+GGG | - | chr8.2:306506-306525 | MS.gene95055:CDS | 55.0% | |
| !! | TGGAGGAAGCATCCCATGCT+TGG | - | chr8.2:306050-306069 | MS.gene95055:intron | 55.0% |
| !!! | GGCATCGCTCTTGTCGCTTT+TGG | - | chr8.2:306104-306123 | MS.gene95055:intron | 55.0% |
| ACAAGAGCGATGCCGAGACC+AGG | + | chr8.2:306098-306117 | None:intergenic | 60.0% | |
| CAGATGCGTCATGCCACTCC+TGG | - | chr8.2:306077-306096 | MS.gene95055:intron | 60.0% | |
| CGTCATGCCACTCCTGGTCT+CGG | - | chr8.2:306083-306102 | MS.gene95055:intron | 60.0% | |
| AGCGATGCCGAGACCAGGAG+TGG | + | chr8.2:306093-306112 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 305999 | 306536 | 305999 | ID=MS.gene95055 |
| chr8.2 | mRNA | 305999 | 306536 | 305999 | ID=MS.gene95055.t1;Parent=MS.gene95055 |
| chr8.2 | exon | 306345 | 306536 | 306345 | ID=MS.gene95055.t1.exon1;Parent=MS.gene95055.t1 |
| chr8.2 | CDS | 306345 | 306536 | 306345 | ID=cds.MS.gene95055.t1;Parent=MS.gene95055.t1 |
| chr8.2 | exon | 305999 | 306019 | 305999 | ID=MS.gene95055.t1.exon2;Parent=MS.gene95055.t1 |
| chr8.2 | CDS | 305999 | 306019 | 305999 | ID=cds.MS.gene95055.t1;Parent=MS.gene95055.t1 |
| Gene Sequence |
| Protein sequence |
