Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95084.t1 | KEH32660.1 | 53.2 | 141 | 28 | 3 | 1 | 103 | 1 | 141 | 2.10E-23 | 119.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95084.t1 | A0A072V3C1 | 53.2 | 141 | 28 | 3 | 1 | 103 | 1 | 141 | 1.5e-23 | 119.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053963 | MS.gene95084 | 0.801452 | 8.95E-49 | -1.69E-46 |
| MS.gene056776 | MS.gene95084 | 0.814973 | 1.17E-51 | -1.69E-46 |
| MS.gene057208 | MS.gene95084 | 0.80416 | 2.47E-49 | -1.69E-46 |
| MS.gene060365 | MS.gene95084 | 0.809443 | 1.89E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95084.t1 | MTR_4g133280 | 51.131 | 221 | 49 | 5 | 1 | 183 | 1 | 200 | 1.62e-51 | 165 |
| MS.gene95084.t1 | MTR_4g133280 | 53.191 | 141 | 28 | 3 | 1 | 103 | 1 | 141 | 6.71e-38 | 129 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACAACTCCACTTCCCTTCT+TGG | 0.225568 | 8.2:-610351 | None:intergenic |
| CTACGTTTCCCACTGATAAT+AGG | 0.269442 | 8.2:-609708 | None:intergenic |
| TAGTGGAGTTGTCACTTCTA+AGG | 0.313166 | 8.2:+610388 | MS.gene95084:CDS |
| AAAACTCAAAACTACTGTTA+TGG | 0.398181 | 8.2:+609737 | MS.gene95084:CDS |
| ATGAAAGAGATAAGGAAGTC+AGG | 0.429458 | 8.2:+610177 | MS.gene95084:CDS |
| CTGCATTGTCTGCAGTGTCC+TGG | 0.444540 | 8.2:-609815 | None:intergenic |
| TTCTAAAGGTGATCATGTCC+TGG | 0.454471 | 8.2:+609680 | MS.gene95084:CDS |
| GTCAGGATAAATTCAAATCC+TGG | 0.455998 | 8.2:+610194 | MS.gene95084:CDS |
| TGATCCTTTCACTGAAGAGC+TGG | 0.462276 | 8.2:+609945 | MS.gene95084:CDS |
| AATGACACCTGAGATGCTTA+AGG | 0.475276 | 8.2:+610056 | MS.gene95084:CDS |
| CTCAAAACTACTGTTATGGA+TGG | 0.487863 | 8.2:+609741 | MS.gene95084:CDS |
| AAGTGGAGTTGTCACTCCTA+AGG | 0.488445 | 8.2:+610361 | MS.gene95084:CDS |
| GCAAGGGAAGTCACATATCC+AGG | 0.497708 | 8.2:+609797 | MS.gene95084:CDS |
| GTCTCCTCCAAGAAGGGAAG+TGG | 0.499665 | 8.2:+610344 | MS.gene95084:CDS |
| AGTGGAGTTGTCACTTCTAA+GGG | 0.511110 | 8.2:+610389 | MS.gene95084:CDS |
| GGAGATGGATGAAAGAGATA+AGG | 0.513093 | 8.2:+610169 | MS.gene95084:CDS |
| AGGGAAGTGGTAGTAGGCAA+GGG | 0.515942 | 8.2:+609781 | MS.gene95084:CDS |
| GAGGGAAGTGGTAGTAGGCA+AGG | 0.518111 | 8.2:+609780 | MS.gene95084:CDS |
| AACTCCACTTCCCTTCTTGG+AGG | 0.521516 | 8.2:-610348 | None:intergenic |
| TTTAGGACCGTTACTATGGA+GGG | 0.521731 | 8.2:+609654 | None:intergenic |
| TCAAGGAGGGAAGTGGTAGT+AGG | 0.524258 | 8.2:+609775 | MS.gene95084:CDS |
| ACAACTCCACTAGCTCCCTT+AGG | 0.528809 | 8.2:-610377 | None:intergenic |
| TCCTGGAGGTCCTAGAAAAG+AGG | 0.539090 | 8.2:+610211 | MS.gene95084:CDS |
| ACTATGGAGGGAAGTTCTAA+AGG | 0.549995 | 8.2:+609666 | None:intergenic |
| AGCTCCAGCTCTTCAGTGAA+AGG | 0.552717 | 8.2:-609949 | None:intergenic |
| GAACTTGTCAAGGAGGGAAG+TGG | 0.557621 | 8.2:+609768 | MS.gene95084:CDS |
| AAGAGTAGTCTCCTCCAAGA+AGG | 0.563577 | 8.2:+610337 | MS.gene95084:CDS |
| TCTAAAGGTGATCATGTCCT+GGG | 0.566442 | 8.2:+609681 | MS.gene95084:CDS |
| AGTGGAGTTGTCACTCCTAA+GGG | 0.567341 | 8.2:+610362 | MS.gene95084:CDS |
| ACAGGTTAGGTATCTGGAGA+TGG | 0.571644 | 8.2:+610154 | MS.gene95084:intron |
| GATGCACTTGAGATTTCTGA+TGG | 0.581693 | 8.2:+610233 | MS.gene95084:CDS |
| GCTGGAGCTGCAACTTAGAG+AGG | 0.583221 | 8.2:+609963 | MS.gene95084:CDS |
| AAGGGAGCTGGAGGCAACAA+AGG | 0.588532 | 8.2:+610416 | MS.gene95084:CDS |
| AGAGTAGTCTCCTCCAAGAA+GGG | 0.590436 | 8.2:+610338 | MS.gene95084:CDS |
| GGATGGAACTGAACTTGTCA+AGG | 0.596328 | 8.2:+609758 | MS.gene95084:CDS |
| GTCACTCCTAAGGGAGCTAG+TGG | 0.597101 | 8.2:+610371 | MS.gene95084:CDS |
| AAATAAACCTGAAGATGAGG+AGG | 0.600134 | 8.2:+609897 | MS.gene95084:intron |
| CAGGACACTGCAGACAATGC+AGG | 0.601280 | 8.2:+609816 | MS.gene95084:CDS |
| TGGGAAAACCTATTATCAGT+GGG | 0.604807 | 8.2:+609700 | MS.gene95084:CDS |
| CTGGGAAAACCTATTATCAG+TGG | 0.606836 | 8.2:+609699 | MS.gene95084:CDS |
| GTGGAGTTGTCACTTCTAAG+GGG | 0.614783 | 8.2:+610390 | MS.gene95084:CDS |
| GGAACTGAACTTGTCAAGGA+GGG | 0.640908 | 8.2:+609762 | MS.gene95084:CDS |
| GTGACTCAAGCAGTAAGACT+CGG | 0.647491 | 8.2:+610279 | MS.gene95084:CDS |
| TGGAACTGAACTTGTCAAGG+AGG | 0.668234 | 8.2:+609761 | MS.gene95084:CDS |
| TGGAGTTGTCACTTCTAAGG+GGG | 0.669080 | 8.2:+610391 | MS.gene95084:CDS |
| AGGATAAATTCAAATCCTGG+AGG | 0.721340 | 8.2:+610197 | MS.gene95084:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATAATTTTATACAGGTT+AGG | + | chr8.2:610141-610160 | MS.gene95084:intron | 15.0% |
| !! | CAACATTATCTATTCAATTA+AGG | - | chr8.2:610099-610118 | None:intergenic | 20.0% |
| ! | AAAACTCAAAACTACTGTTA+TGG | + | chr8.2:609737-609756 | MS.gene95084:CDS | 25.0% |
| !!! | CTATTGAATTGAATTTTGAC+TGG | + | chr8.2:609989-610008 | MS.gene95084:CDS | 25.0% |
| !!! | TTATTTCTGAAGTTAATAGC+AGG | - | chr8.2:609883-609902 | None:intergenic | 25.0% |
| ! | TATTTTACCTTAAGCATCTC+AGG | - | chr8.2:610066-610085 | None:intergenic | 30.0% |
| ! | TTTTATACAGGTTAGGTATC+TGG | + | chr8.2:610148-610167 | MS.gene95084:intron | 30.0% |
| !!! | CAGTAGTTTTGAGTTTTCTT+TGG | - | chr8.2:609734-609753 | None:intergenic | 30.0% |
| !!! | TTGAATTGAATTTTGACTGG+AGG | + | chr8.2:609992-610011 | MS.gene95084:CDS | 30.0% |
| AAATAAACCTGAAGATGAGG+AGG | + | chr8.2:609897-609916 | MS.gene95084:intron | 35.0% | |
| AGGATAAATTCAAATCCTGG+AGG | + | chr8.2:610197-610216 | MS.gene95084:CDS | 35.0% | |
| ATGAAAGAGATAAGGAAGTC+AGG | + | chr8.2:610177-610196 | MS.gene95084:CDS | 35.0% | |
| CAGAAATAAACCTGAAGATG+AGG | + | chr8.2:609894-609913 | MS.gene95084:intron | 35.0% | |
| CTCAAAACTACTGTTATGGA+TGG | + | chr8.2:609741-609760 | MS.gene95084:CDS | 35.0% | |
| GTCAGGATAAATTCAAATCC+TGG | + | chr8.2:610194-610213 | MS.gene95084:CDS | 35.0% | |
| TGGGAAAACCTATTATCAGT+GGG | + | chr8.2:609700-609719 | MS.gene95084:CDS | 35.0% | |
| AATGACACCTGAGATGCTTA+AGG | + | chr8.2:610056-610075 | MS.gene95084:CDS | 40.0% | |
| CTACGTTTCCCACTGATAAT+AGG | - | chr8.2:609711-609730 | None:intergenic | 40.0% | |
| CTGGGAAAACCTATTATCAG+TGG | + | chr8.2:609699-609718 | MS.gene95084:CDS | 40.0% | |
| GATGCACTTGAGATTTCTGA+TGG | + | chr8.2:610233-610252 | MS.gene95084:CDS | 40.0% | |
| GGAGATGGATGAAAGAGATA+AGG | + | chr8.2:610169-610188 | MS.gene95084:CDS | 40.0% | |
| TCTAAAGGTGATCATGTCCT+GGG | + | chr8.2:609681-609700 | MS.gene95084:CDS | 40.0% | |
| ! | AGTGGAGTTGTCACTTCTAA+GGG | + | chr8.2:610389-610408 | MS.gene95084:CDS | 40.0% |
| ! | CACTGATAATAGGTTTTCCC+AGG | - | chr8.2:609701-609720 | None:intergenic | 40.0% |
| ! | TAGTGGAGTTGTCACTTCTA+AGG | + | chr8.2:610388-610407 | MS.gene95084:CDS | 40.0% |
| ! | TCAAGTGCATCCTCTTTTCT+AGG | - | chr8.2:610224-610243 | None:intergenic | 40.0% |
| ! | TTCTAAAGGTGATCATGTCC+TGG | + | chr8.2:609680-609699 | MS.gene95084:CDS | 40.0% |
| AAGAGTAGTCTCCTCCAAGA+AGG | + | chr8.2:610337-610356 | MS.gene95084:CDS | 45.0% | |
| AAGTGGAGTTGTCACTCCTA+AGG | + | chr8.2:610361-610380 | MS.gene95084:CDS | 45.0% | |
| ACAGGTTAGGTATCTGGAGA+TGG | + | chr8.2:610154-610173 | MS.gene95084:intron | 45.0% | |
| AGAGTAGTCTCCTCCAAGAA+GGG | + | chr8.2:610338-610357 | MS.gene95084:CDS | 45.0% | |
| AGTGGAGTTGTCACTCCTAA+GGG | + | chr8.2:610362-610381 | MS.gene95084:CDS | 45.0% | |
| GGAACTGAACTTGTCAAGGA+GGG | + | chr8.2:609762-609781 | MS.gene95084:CDS | 45.0% | |
| GGATGGAACTGAACTTGTCA+AGG | + | chr8.2:609758-609777 | MS.gene95084:CDS | 45.0% | |
| GTGACTCAAGCAGTAAGACT+CGG | + | chr8.2:610279-610298 | MS.gene95084:CDS | 45.0% | |
| TGATCCTTTCACTGAAGAGC+TGG | + | chr8.2:609945-609964 | MS.gene95084:CDS | 45.0% | |
| TGGAACTGAACTTGTCAAGG+AGG | + | chr8.2:609761-609780 | MS.gene95084:CDS | 45.0% | |
| ! | GTGGAGTTGTCACTTCTAAG+GGG | + | chr8.2:610390-610409 | MS.gene95084:CDS | 45.0% |
| ! | TGGAGTTGTCACTTCTAAGG+GGG | + | chr8.2:610391-610410 | MS.gene95084:CDS | 45.0% |
| !!! | AATTATTTATAATTTTATAC+AGG | + | chr8.2:610136-610155 | MS.gene95084:intron | 5.0% |
| AACTCCACTTCCCTTCTTGG+AGG | - | chr8.2:610351-610370 | None:intergenic | 50.0% | |
| ACAACTCCACTAGCTCCCTT+AGG | - | chr8.2:610380-610399 | None:intergenic | 50.0% | |
| AGCTCCAGCTCTTCAGTGAA+AGG | - | chr8.2:609952-609971 | None:intergenic | 50.0% | |
| AGGGAAGTGGTAGTAGGCAA+GGG | + | chr8.2:609781-609800 | MS.gene95084:CDS | 50.0% | |
| GAACTTGTCAAGGAGGGAAG+TGG | + | chr8.2:609768-609787 | MS.gene95084:CDS | 50.0% | |
| GACAACTCCACTTCCCTTCT+TGG | - | chr8.2:610354-610373 | None:intergenic | 50.0% | |
| GCAAGGGAAGTCACATATCC+AGG | + | chr8.2:609797-609816 | MS.gene95084:CDS | 50.0% | |
| TCAAGGAGGGAAGTGGTAGT+AGG | + | chr8.2:609775-609794 | MS.gene95084:CDS | 50.0% | |
| TCCTGGAGGTCCTAGAAAAG+AGG | + | chr8.2:610211-610230 | MS.gene95084:CDS | 50.0% | |
| ! | GTCACTTCTAAGGGGGCTAA+GGG | + | chr8.2:610398-610417 | MS.gene95084:CDS | 50.0% |
| ! | TGTCACTTCTAAGGGGGCTA+AGG | + | chr8.2:610397-610416 | MS.gene95084:CDS | 50.0% |
| !!! | TCCTCTTTTCTAGGACCTCC+AGG | - | chr8.2:610215-610234 | None:intergenic | 50.0% |
| AAGGGAGCTGGAGGCAACAA+AGG | + | chr8.2:610416-610435 | MS.gene95084:CDS | 55.0% | |
| CACAGCTCCTCCTCATCTTC+AGG | - | chr8.2:609907-609926 | None:intergenic | 55.0% | |
| CAGGACACTGCAGACAATGC+AGG | + | chr8.2:609816-609835 | MS.gene95084:CDS | 55.0% | |
| CTGCATTGTCTGCAGTGTCC+TGG | - | chr8.2:609818-609837 | None:intergenic | 55.0% | |
| GAGGGAAGTGGTAGTAGGCA+AGG | + | chr8.2:609780-609799 | MS.gene95084:CDS | 55.0% | |
| GCTGGAGCTGCAACTTAGAG+AGG | + | chr8.2:609963-609982 | MS.gene95084:CDS | 55.0% | |
| GTCACTCCTAAGGGAGCTAG+TGG | + | chr8.2:610371-610390 | MS.gene95084:CDS | 55.0% | |
| GTCTCCTCCAAGAAGGGAAG+TGG | + | chr8.2:610344-610363 | MS.gene95084:CDS | 55.0% | |
| TCTAAGGGGGCTAAGGGAGC+TGG | + | chr8.2:610404-610423 | MS.gene95084:CDS | 60.0% | |
| AAGGGGGCTAAGGGAGCTGG+AGG | + | chr8.2:610407-610426 | MS.gene95084:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 609669 | 610469 | 609669 | ID=MS.gene95084 |
| chr8.2 | mRNA | 609669 | 610469 | 609669 | ID=MS.gene95084.t1;Parent=MS.gene95084 |
| chr8.2 | exon | 609669 | 609837 | 609669 | ID=MS.gene95084.t1.exon1;Parent=MS.gene95084.t1 |
| chr8.2 | CDS | 609669 | 609837 | 609669 | ID=cds.MS.gene95084.t1;Parent=MS.gene95084.t1 |
| chr8.2 | exon | 609911 | 610077 | 609911 | ID=MS.gene95084.t1.exon2;Parent=MS.gene95084.t1 |
| chr8.2 | CDS | 609911 | 610077 | 609911 | ID=cds.MS.gene95084.t1;Parent=MS.gene95084.t1 |
| chr8.2 | exon | 610158 | 610469 | 610158 | ID=MS.gene95084.t1.exon3;Parent=MS.gene95084.t1 |
| chr8.2 | CDS | 610158 | 610469 | 610158 | ID=cds.MS.gene95084.t1;Parent=MS.gene95084.t1 |
| Gene Sequence |
| Protein sequence |
