AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95500.t1 MTR_5g044770 95.824 431 18 0 1 431 1 431 0.0 850
MS.gene95500.t1 MTR_8g096990 72.222 432 119 1 1 431 1 432 0.0 643
MS.gene95500.t1 MTR_5g080960 71.396 437 116 5 1 431 1 434 0.0 626
MS.gene95500.t1 MTR_5g080890 70.938 437 118 5 1 431 1 434 0.0 625
MS.gene95500.t1 MTR_8g038280 68.807 436 131 2 1 431 1 436 0.0 615
MS.gene95500.t1 MTR_8g038250 68.037 438 111 3 1 431 1 416 0.0 598
MS.gene95500.t1 MTR_6g090290 63.532 436 153 3 1 430 1 436 0.0 543
MS.gene95500.t1 MTR_8g038310 62.731 432 140 5 1 430 1 413 0.0 540
MS.gene95500.t1 MTR_4g109760 62.587 433 126 6 1 431 1 399 4.55e-173 490
MS.gene95500.t1 MTR_3g028120 51.551 419 194 5 22 431 21 439 9.13e-144 417
MS.gene95500.t1 MTR_2g067830 53.695 406 181 6 32 430 1 406 1.90e-141 410
MS.gene95500.t1 MTR_5g038360 48.730 433 207 7 9 430 8 436 2.81e-138 403
MS.gene95500.t1 MTR_6g088760 52.068 411 185 7 1 401 1 409 1.90e-135 399
MS.gene95500.t1 MTR_0024s0040 50.121 413 191 7 30 430 1 410 7.03e-131 383
MS.gene95500.t1 MTR_2g105300 50.868 403 169 8 32 427 1 381 1.07e-126 372
MS.gene95500.t1 MTR_5g044570 46.189 433 192 10 9 430 9 411 1.86e-124 367
MS.gene95500.t1 MTR_0036s0050 42.792 437 228 12 9 430 6 435 3.21e-107 324
MS.gene95500.t1 MTR_0036s0070 40.972 432 234 11 9 430 6 426 3.23e-105 318
MS.gene95500.t1 MTR_5g008070 40.097 414 230 7 21 428 23 424 2.94e-99 303
MS.gene95500.t1 MTR_2g059490 45.983 361 185 7 77 428 49 408 2.53e-98 301
MS.gene95500.t1 MTR_0036s0110 39.159 452 243 12 1 430 1 442 6.34e-96 295
MS.gene95500.t1 MTR_5g008150 40.789 380 214 8 59 430 36 412 5.00e-92 284
MS.gene95500.t1 MTR_2g059460 43.099 355 185 9 86 430 59 406 1.09e-89 278
MS.gene95500.t1 MTR_5g008110 41.525 354 195 7 85 430 66 415 4.76e-88 274
MS.gene95500.t1 MTR_3g097150 36.854 445 251 14 6 431 16 449 3.18e-82 260
MS.gene95500.t1 MTR_1g060750 35.572 402 221 11 40 430 2 376 2.81e-70 227
MS.gene95500.t1 MTR_4g045857 40.193 311 149 7 6 310 9 288 4.28e-64 208
MS.gene95500.t1 MTR_1g115210 37.677 353 200 11 85 428 145 486 3.84e-61 206
MS.gene95500.t1 MTR_5g008090 38.047 297 173 7 142 430 21 314 4.76e-59 196
MS.gene95500.t1 MTR_1g039270 35.876 354 206 11 85 428 127 469 6.29e-58 197
MS.gene95500.t1 MTR_7g117430 32.864 426 249 15 25 428 74 484 8.92e-58 197
MS.gene95500.t1 MTR_6g085010 34.595 370 221 11 66 428 136 491 1.66e-52 183
MS.gene95500.t1 MTR_1g111350 32.458 419 239 16 21 428 82 467 5.18e-52 181
MS.gene95500.t1 MTR_2g028190 31.429 455 268 15 9 428 6 451 1.64e-48 172
MS.gene95500.t1 MTR_3g046300 29.759 457 274 13 11 431 16 461 3.81e-48 171
MS.gene95500.t1 MTR_6g088765 40.856 257 109 7 160 414 3 218 5.42e-47 161
MS.gene95500.t1 MTR_4g007740 31.671 401 236 14 49 428 94 477 2.87e-46 166
MS.gene95500.t1 MTR_5g091130 31.915 376 234 11 77 431 182 556 2.07e-44 162
MS.gene95500.t1 MTR_3g053710 32.507 363 220 12 82 428 113 466 5.97e-42 154
MS.gene95500.t1 MTR_8g103880 29.565 345 220 12 87 421 117 448 1.92e-36 139
MS.gene95500.t1 MTR_2g070430 28.540 459 276 17 6 426 7 451 7.73e-35 135
MS.gene95500.t1 MTR_2g055840 27.704 379 236 12 78 428 5 373 1.00e-33 130
MS.gene95500.t1 MTR_3g068000 28.750 400 227 14 71 428 58 441 8.68e-32 126
MS.gene95500.t1 MTR_3g053980 30.133 375 221 12 75 428 131 485 1.15e-30 123
MS.gene95500.t1 MTR_7g080230 27.689 437 257 18 32 428 13 430 4.72e-30 121
MS.gene95500.t1 MTR_7g105850 28.992 476 271 21 6 430 8 467 1.42e-28 117
MS.gene95500.t1 MTR_4g008110 51.724 116 33 1 158 273 45 137 8.65e-28 107
MS.gene95500.t1 MTR_8g075090 28.533 368 220 16 87 430 113 461 1.16e-26 112
MS.gene95500.t1 MTR_7g062360 27.000 400 236 15 72 431 45 428 1.32e-26 111
MS.gene95500.t1 MTR_4g095270 26.633 398 237 19 57 431 61 426 1.64e-26 112
MS.gene95500.t1 MTR_8g075090 28.689 366 218 16 87 428 113 459 2.31e-26 111
MS.gene95500.t1 MTR_4g095270 26.633 398 237 19 57 431 61 426 2.40e-26 112
MS.gene95500.t1 MTR_3g065160 25.806 372 230 12 88 431 76 429 3.70e-26 110
MS.gene95500.t1 MTR_6g084710 27.033 418 249 20 49 428 151 550 4.61e-26 110
MS.gene95500.t1 MTR_5g022880 26.984 441 266 17 27 428 38 461 7.75e-26 110
MS.gene95500.t1 MTR_5g016260 24.937 397 239 18 57 428 69 431 1.01e-25 110
MS.gene95500.t1 MTR_5g016260 24.937 397 239 18 57 428 69 431 1.40e-25 109
MS.gene95500.t1 MTR_6g084710 28.530 347 210 17 49 375 151 479 1.61e-25 108
MS.gene95500.t1 MTR_8g094590 27.114 402 231 17 65 431 53 427 4.26e-25 107
MS.gene95500.t1 MTR_8g075090 30.060 336 192 16 92 403 118 434 9.22e-25 106
MS.gene95500.t1 MTR_1g061940 29.867 375 220 18 85 428 82 444 6.68e-24 104
MS.gene95500.t1 MTR_2g103340 44.828 116 20 1 21 136 34 105 1.65e-23 97.4
MS.gene95500.t1 MTR_2g103340 45.455 77 37 2 349 425 88 159 1.07e-11 63.9
MS.gene95500.t1 MTR_8g094590 28.760 379 215 17 65 416 53 403 1.66e-23 102
MS.gene95500.t1 MTR_1g078240 26.203 374 234 14 85 428 68 429 1.69e-23 102
MS.gene95500.t1 MTR_3g087780 26.490 453 260 22 24 428 23 450 2.45e-23 102
MS.gene95500.t1 MTR_2g084550 30.645 372 220 15 85 428 76 437 3.29e-23 102
MS.gene95500.t1 MTR_1g078240 26.400 375 233 15 85 428 68 430 4.85e-23 101
MS.gene95500.t1 MTR_8g094590 30.851 282 158 12 65 333 53 310 1.84e-22 97.4
MS.gene95500.t1 MTR_3g096930 25.132 378 229 17 85 431 83 437 2.84e-22 99.0
MS.gene95500.t1 MTR_7g021510 26.385 379 228 16 85 428 71 433 3.72e-22 99.0
MS.gene95500.t1 MTR_7g102750 28.760 379 217 20 85 428 80 440 5.80e-22 98.6
MS.gene95500.t1 MTR_1g029810 25.729 377 230 16 85 431 56 412 6.80e-22 97.8
MS.gene95500.t1 MTR_1g029810 25.995 377 229 17 85 431 56 412 7.40e-22 97.4
MS.gene95500.t1 MTR_2g063110 23.750 480 273 18 27 428 44 508 2.61e-21 96.7
MS.gene95500.t1 MTR_4g056420 28.032 371 230 16 85 428 76 436 5.97e-21 95.1
MS.gene95500.t1 MTR_5g061690 29.226 349 199 20 93 416 108 433 9.87e-21 94.7
MS.gene95500.t1 MTR_4g023650 25.768 423 250 20 50 431 23 422 1.18e-20 94.0
MS.gene95500.t1 MTR_8g075010 25.474 369 234 12 87 431 101 452 1.66e-20 94.4
MS.gene95500.t1 MTR_4g100990 25.122 410 237 18 84 431 72 473 1.97e-20 93.6
MS.gene95500.t1 MTR_1g078240 26.861 309 192 12 85 371 68 364 8.83e-20 90.9
MS.gene95500.t1 MTR_5g077850 25.132 378 229 11 88 431 83 440 6.10e-19 89.0
MS.gene95500.t1 MTR_7g102750 28.940 349 196 19 85 399 80 410 6.89e-19 89.0
MS.gene95500.t1 MTR_3g087780 29.154 319 188 16 129 428 9 308 8.97e-19 88.2
MS.gene95500.t1 MTR_3g087780 29.154 319 188 16 129 428 45 344 1.24e-18 87.8
MS.gene95500.t1 MTR_0945s0010 37.398 123 73 3 309 428 1 122 3.72e-18 81.6
MS.gene95500.t1 MTR_1g037630 26.241 423 271 17 26 428 27 428 5.92e-18 85.9
MS.gene95500.t1 MTR_3g014290 26.912 353 230 12 87 428 81 416 6.17e-18 85.9
MS.gene95500.t1 MTR_7g080230 26.571 350 212 15 110 428 25 360 1.07e-17 85.1
MS.gene95500.t1 MTR_4g023630 23.990 421 260 19 50 431 23 422 3.71e-17 83.6
MS.gene95500.t1 MTR_8g039540 26.590 346 227 12 82 416 92 421 5.42e-16 80.1
MS.gene95500.t1 MTR_5g061690 28.912 294 172 18 138 416 23 294 1.41e-15 78.6
MS.gene95500.t1 MTR_3g100500 26.823 384 244 15 52 420 58 419 2.11e-13 72.0
MS.gene95500.t1 MTR_5g077920 24.165 389 251 13 73 430 53 428 2.33e-13 72.0
MS.gene95500.t1 MTR_3g006820 22.680 388 230 16 85 431 57 415 3.80e-12 68.2
MS.gene95500.t1 MTR_2g037710 27.805 205 125 7 230 428 18 205 2.07e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95500.t1 AT1G64830 49.877 407 200 4 25 428 23 428 2.18e-136 398
MS.gene95500.t1 AT5G33340 49.020 408 203 5 28 431 31 437 5.38e-129 380
MS.gene95500.t1 AT2G35615 43.478 437 225 6 14 428 7 443 1.53e-111 335
MS.gene95500.t1 AT1G31450 42.697 445 234 8 1 428 1 441 5.16e-107 323
MS.gene95500.t1 AT2G28010 40.230 435 207 12 1 428 3 391 1.47e-88 275
MS.gene95500.t1 AT2G28040 41.050 419 195 12 16 428 18 390 7.24e-85 265
MS.gene95500.t1 AT2G28040 43.767 377 183 11 58 428 28 381 1.77e-84 264
MS.gene95500.t1 AT2G28220 39.294 425 203 14 15 428 17 397 1.27e-79 252
MS.gene95500.t1 AT2G28030 43.553 349 168 10 87 428 61 387 1.70e-78 248
MS.gene95500.t1 AT2G28225 42.776 353 169 11 87 428 67 397 2.86e-78 248
MS.gene95500.t1 AT2G03200 36.806 432 236 13 25 431 42 461 7.08e-74 239
MS.gene95500.t1 AT3G18490 32.160 426 258 14 18 428 91 500 4.81e-58 198
MS.gene95500.t1 AT3G20015 31.724 435 256 16 20 428 51 470 1.69e-57 196
MS.gene95500.t1 AT1G01300 36.056 355 206 12 85 428 140 484 2.60e-56 193
MS.gene95500.t1 AT3G61820 34.711 363 206 13 85 428 133 483 3.30e-52 182
MS.gene95500.t1 AT1G25510 31.116 421 257 14 22 428 82 483 7.85e-50 176
MS.gene95500.t1 AT4G30030 33.244 373 221 12 70 428 61 419 8.80e-50 174
MS.gene95500.t1 AT5G10760 34.243 403 235 14 31 428 87 464 7.85e-48 170
MS.gene95500.t1 AT2G23945 27.391 460 279 15 9 431 10 451 7.91e-47 167
MS.gene95500.t1 AT5G10770 33.604 369 215 12 75 428 120 473 1.68e-46 167
MS.gene95500.t1 AT3G12700 29.930 431 278 14 16 428 36 460 9.66e-44 159
MS.gene95500.t1 AT3G25700 28.066 424 258 15 39 428 39 449 3.28e-43 157
MS.gene95500.t1 AT2G42980 31.759 381 231 14 77 431 150 527 3.29e-42 156
MS.gene95500.t1 AT4G30040 30.995 442 262 16 6 423 5 427 6.01e-42 154
MS.gene95500.t1 AT3G59080 30.607 379 236 12 77 431 160 535 3.40e-41 154
MS.gene95500.t1 AT1G79720 29.120 443 265 17 20 428 55 482 4.46e-39 147
MS.gene95500.t1 AT5G36260 29.333 375 217 15 85 428 76 433 1.03e-35 137
MS.gene95500.t1 AT3G02740 28.796 382 222 17 76 428 79 439 1.67e-32 129
MS.gene95500.t1 AT3G02740 28.571 371 220 16 87 428 10 364 3.41e-32 127
MS.gene95500.t1 AT3G59080 29.144 374 212 11 77 431 160 499 9.29e-32 127
MS.gene95500.t1 AT2G39710 28.267 375 213 14 89 423 67 425 1.94e-29 119
MS.gene95500.t1 AT1G65240 27.882 373 222 16 85 428 72 426 2.76e-29 119
MS.gene95500.t1 AT5G43100 26.168 428 260 17 28 428 19 417 2.15e-26 111
MS.gene95500.t1 AT3G54400 27.557 352 218 13 62 396 56 387 4.20e-26 109
MS.gene95500.t1 AT5G02190 27.056 377 215 15 96 428 82 442 7.70e-26 109
MS.gene95500.t1 AT5G43100 26.168 428 260 17 28 428 19 417 7.94e-26 110
MS.gene95500.t1 AT2G17760 27.078 421 258 18 32 428 54 449 2.42e-24 105
MS.gene95500.t1 AT5G37540 25.737 373 230 13 88 428 81 438 2.43e-24 105
MS.gene95500.t1 AT1G09750 27.247 356 238 12 85 428 102 448 5.26e-24 103
MS.gene95500.t1 AT5G45120 27.228 404 226 18 87 429 83 479 8.47e-24 103
MS.gene95500.t1 AT1G49050 25.978 358 225 16 104 430 49 397 1.12e-23 102
MS.gene95500.t1 AT1G49050 25.783 415 259 19 49 430 172 570 1.26e-23 103
MS.gene95500.t1 AT1G44130 25.500 400 243 17 67 431 23 402 1.80e-23 102
MS.gene95500.t1 AT3G50050 25.612 449 277 19 12 428 13 436 1.94e-23 103
MS.gene95500.t1 AT1G05840 26.667 375 230 16 85 428 78 438 2.64e-23 102
MS.gene95500.t1 AT2G36670 28.267 375 223 19 85 428 98 457 2.87e-23 102
MS.gene95500.t1 AT2G36670 28.010 382 229 19 78 428 25 391 3.77e-23 101
MS.gene95500.t1 AT2G36670 28.010 382 229 19 78 428 25 391 3.77e-23 101
MS.gene95500.t1 AT4G33490 25.369 406 239 17 63 428 30 411 4.64e-23 101
MS.gene95500.t1 AT2G36670 28.198 383 225 20 81 428 95 462 5.09e-23 101
MS.gene95500.t1 AT1G66180 27.005 374 226 14 88 431 73 429 2.14e-22 99.4
MS.gene95500.t1 AT5G22850 30.263 380 211 19 85 428 79 440 7.66e-22 98.2
MS.gene95500.t1 AT3G51350 25.951 447 266 21 28 430 29 454 1.16e-21 97.8
MS.gene95500.t1 AT1G49050 27.063 303 192 13 49 338 172 458 1.11e-20 94.4
MS.gene95500.t1 AT3G52500 29.517 393 215 19 85 428 88 467 1.73e-20 94.0
MS.gene95500.t1 AT4G33490 32.432 185 102 8 63 232 27 203 2.26e-20 92.8
MS.gene95500.t1 AT1G08210 28.533 375 221 18 85 428 130 488 2.58e-20 93.6
MS.gene95500.t1 AT4G12920 25.995 377 247 14 62 428 33 387 3.25e-20 92.4
MS.gene95500.t1 AT1G08210 28.533 375 221 18 85 428 116 474 3.37e-20 93.2
MS.gene95500.t1 AT1G08210 28.533 375 221 18 85 428 82 440 3.38e-20 93.2
MS.gene95500.t1 AT3G51360 26.739 460 270 21 11 428 1 435 4.84e-20 92.8
MS.gene95500.t1 AT3G51360 26.739 460 270 21 11 428 11 445 5.28e-20 92.4
MS.gene95500.t1 AT3G51360 28.650 363 212 17 92 428 10 351 6.73e-20 91.7
MS.gene95500.t1 AT1G77480 24.409 381 231 15 85 431 65 422 1.05e-19 91.3
MS.gene95500.t1 AT1G77480 24.409 381 231 15 85 431 65 422 1.42e-19 91.3
MS.gene95500.t1 AT5G10080 25.000 472 275 25 11 428 7 453 6.97e-19 89.4
MS.gene95500.t1 AT4G35880 24.255 470 286 18 8 431 9 454 3.17e-18 87.4
MS.gene95500.t1 AT3G51360 27.316 421 241 20 12 392 12 407 1.31e-17 85.1
MS.gene95500.t1 AT3G51360 27.316 421 241 20 12 392 2 397 1.45e-17 84.7
MS.gene95500.t1 AT1G08210 28.698 338 196 17 85 393 82 403 8.46e-17 82.8
MS.gene95500.t1 AT3G51360 26.781 407 237 18 12 381 2 384 1.00e-16 82.0
MS.gene95500.t1 AT3G51360 26.781 407 237 18 12 381 12 394 1.09e-16 82.0
MS.gene95500.t1 AT5G07030 28.354 328 192 13 87 396 115 417 9.29e-16 79.3
MS.gene95500.t1 AT3G51340 25.627 359 230 16 92 430 108 449 2.52e-15 78.2
MS.gene95500.t1 AT4G35880 24.153 443 266 17 8 404 9 427 3.00e-15 77.8
MS.gene95500.t1 AT3G25700 25.521 192 122 5 39 217 39 222 3.45e-15 77.0
MS.gene95500.t1 AT3G51360 26.211 351 209 14 11 332 11 340 4.83e-15 76.6
MS.gene95500.t1 AT3G51360 28.931 318 188 16 128 428 13 309 1.09e-14 75.5
MS.gene95500.t1 AT3G51360 28.931 318 188 16 128 428 13 309 1.09e-14 75.5
MS.gene95500.t1 AT3G51340 22.826 368 241 12 92 430 108 461 1.14e-14 76.3
MS.gene95500.t1 AT3G51340 25.070 359 225 15 28 356 30 374 5.46e-14 73.6
MS.gene95500.t1 AT3G51340 25.140 358 224 15 28 355 30 373 1.04e-13 72.8
MS.gene95500.t1 AT4G16563 25.416 421 231 18 83 428 79 491 2.75e-12 68.9
MS.gene95500.t1 AT3G42550 31.731 104 69 1 87 188 78 181 8.15e-12 67.0
MS.gene95500.t1 AT5G24820 23.514 387 237 14 75 429 41 400 1.57e-11 66.2

Find 82 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTCATGTTTATACTAATT+TGG 0.143876 5.2:-35097800 MS.gene95500:CDS
GGAGATGCCGCTATGGTTTC+TGG 0.152074 5.2:-35097991 MS.gene95500:CDS
AAGACGAGCAAACTCAATTT+TGG 0.203851 5.2:-35098012 MS.gene95500:CDS
TTAAAATGTGCAGTGATTAT+AGG 0.235754 5.2:+35097679 None:intergenic
ACATGAGCAAAGGTACTAAT+TGG 0.237962 5.2:+35097634 None:intergenic
ATACAGGTAGTGACATTATT+TGG 0.244218 5.2:-35098449 MS.gene95500:CDS
CAAAGGTTCAATAGTTGATT+AGG 0.274777 5.2:+35097733 None:intergenic
ACAACACCATATACATTAAA+AGG 0.278090 5.2:+35098474 None:intergenic
TCATTTCCTAAAACTGTTAT+CGG 0.289191 5.2:-35098186 MS.gene95500:CDS
AAAGAAAAGAAGGGTAATAA+AGG 0.291962 5.2:+35098749 None:intergenic
TCTCAAACTGGTCCCATCTT+TGG 0.292790 5.2:-35097577 MS.gene95500:CDS
TTACTGTTTGTTGCCGTTGA+TGG 0.300361 5.2:-35098046 MS.gene95500:CDS
TTGACATATACAGTTGATTC+AGG 0.308336 5.2:+35098537 None:intergenic
GGTATAGTTGGTCTTGGAAT+TGG 0.333564 5.2:-35098120 MS.gene95500:CDS
TGAGTATTTGATGACTTATT+CGG 0.350299 5.2:-35098508 MS.gene95500:CDS
TAAATGATTGGCACGATTGA+TGG 0.356896 5.2:+35098584 None:intergenic
CTTGGCACAACTAAATTTGT+TGG 0.360015 5.2:-35097551 MS.gene95500:CDS
ATGTTTCAAGGGGCAACTTC+CGG 0.393396 5.2:-35098141 MS.gene95500:CDS
GGTAACATCATACTTGATTC+AGG 0.399481 5.2:-35097844 MS.gene95500:CDS
TATGTCTCTTACAACACAAT+TGG 0.409960 5.2:-35098094 MS.gene95500:CDS
GGAATCGGCGGTGGCACAAT+TGG 0.411720 5.2:-35097779 MS.gene95500:CDS
ACACAATTGGAATCTTCAAT+TGG 0.412966 5.2:-35098081 MS.gene95500:CDS
TTGTGCCAAGTTTCCAAAGA+TGG 0.428148 5.2:+35097564 None:intergenic
TGTTTATACTAATTTGGAAT+CGG 0.430186 5.2:-35097794 MS.gene95500:CDS
TGGTCCCATCTTTGGAAACT+TGG 0.432754 5.2:-35097569 MS.gene95500:CDS
ACTTCCGGTATAGTTGGTCT+TGG 0.461194 5.2:-35098126 MS.gene95500:CDS
CCTAAAACTGTTATCGGATG+TGG 0.461217 5.2:-35098180 MS.gene95500:CDS
AATAATACAGGGATGTTTCA+AGG 0.462978 5.2:-35098153 MS.gene95500:CDS
TGCTTGTAGCATTGTTCACA+AGG 0.465026 5.2:+35098414 None:intergenic
AAAAGAAATAATGAAACAAA+AGG 0.465503 5.2:+35098725 None:intergenic
TACTAATTTGGAATCGGCGG+TGG 0.466564 5.2:-35097788 MS.gene95500:CDS
AACAAAAGAATAGAATTTGA+AGG 0.474792 5.2:-35097886 MS.gene95500:CDS
GGATGTGGACACAATAATAC+AGG 0.478375 5.2:-35098165 MS.gene95500:CDS
ACCTTTGCTCATGTTGCTGA+TGG 0.482020 5.2:-35097625 MS.gene95500:CDS
AATTGTGTTGTAAGAGACAT+AGG 0.482732 5.2:+35098096 None:intergenic
ACATTAGAAGCATTTAGTGT+TGG 0.483347 5.2:-35097910 MS.gene95500:CDS
AACAAAAGGAAAAGAAAAGA+AGG 0.487257 5.2:+35098739 None:intergenic
TGCTCATGTTGCTGATGGTG+TGG 0.491769 5.2:-35097620 MS.gene95500:CDS
TGAAACAGAATGACCGGTGG+TGG 0.491841 5.2:+35098206 None:intergenic
GGAATCTTGAAATAAATGAT+TGG 0.494179 5.2:+35098572 None:intergenic
TAGTGTTGAGACTCTTACAT+TGG 0.503954 5.2:-35098232 MS.gene95500:CDS
GCACAACTAAATTTGTTGGT+TGG 0.505438 5.2:-35097547 MS.gene95500:CDS
AAGAAAAGAAGGGTAATAAA+GGG 0.506609 5.2:+35098750 None:intergenic
GAATCAACTGTATATGTCAA+TGG 0.515896 5.2:-35098534 MS.gene95500:CDS
CTTACATTGGATTCCACCAC+CGG 0.521813 5.2:-35098219 MS.gene95500:CDS
CATGAGCAAAGGTACTAATT+GGG 0.521844 5.2:+35097635 None:intergenic
GTATATGGTGTTGTTGATAC+AGG 0.522915 5.2:-35098465 MS.gene95500:CDS
AGGAAATGAAACAGAATGAC+CGG 0.524965 5.2:+35098200 None:intergenic
GGGGCAACTTCCGGTATAGT+TGG 0.526062 5.2:-35098132 MS.gene95500:CDS
GCCGCTATGGTTTCTGGTGA+CGG 0.531191 5.2:-35097985 MS.gene95500:CDS
TAAGCCTAGTGATTGTATCA+AGG 0.531478 5.2:-35097494 MS.gene95500:CDS
TGTGCCAAGTTTCCAAAGAT+GGG 0.540890 5.2:+35097565 None:intergenic
AATTCCAAGACCAACTATAC+CGG 0.550046 5.2:+35098122 None:intergenic
ACAAAAGAATAGAATTTGAA+GGG 0.554803 5.2:-35097885 MS.gene95500:CDS
ATAATACAGGGATGTTTCAA+GGG 0.555636 5.2:-35098152 MS.gene95500:CDS
AGAGTTCTGTTCATTACAAG+AGG 0.561935 5.2:+35098305 None:intergenic
ACATATACAGTTGATTCAGG+TGG 0.563120 5.2:+35098540 None:intergenic
ACAAAAGGAAAAGAAAAGAA+GGG 0.570124 5.2:+35098740 None:intergenic
GGGTTAGATGACAGCGAGGA+GGG 0.574251 5.2:-35097865 MS.gene95500:CDS
CTGATGTGATACTATAACAA+AGG 0.576754 5.2:+35097716 None:intergenic
TGAAGGATTAAATATAGGAG+TGG 0.580266 5.2:+35098389 None:intergenic
CAATTGGAATCTTCAATTGG+TGG 0.584460 5.2:-35098078 MS.gene95500:CDS
TTGATTCAGGTGGATTAGAG+AGG 0.588965 5.2:+35098550 None:intergenic
TGATTCAGGTGGATTAGAGA+GGG 0.593379 5.2:+35098551 None:intergenic
TATTTGATGACTTATTCGGT+TGG 0.593557 5.2:-35098504 MS.gene95500:CDS
TGACATAAGTTAGAAGAGCA+AGG 0.597450 5.2:+35098342 None:intergenic
GTGCAGCATTGACAACATGC+TGG 0.603421 5.2:+35098613 None:intergenic
ACCATCAGCAACATGAGCAA+AGG 0.634882 5.2:+35097624 None:intergenic
TTGTTCGAGTCAACCATCAA+CGG 0.637876 5.2:+35098033 None:intergenic
TTATACTAATTTGGAATCGG+CGG 0.640658 5.2:-35097791 MS.gene95500:CDS
AAATTAGTATAAACATGAGA+CGG 0.651862 5.2:+35097802 None:intergenic
TACACCTTGATACAATCACT+AGG 0.657482 5.2:+35097490 None:intergenic
ACACCATATACATTAAAAGG+AGG 0.660614 5.2:+35098477 None:intergenic
TAATACAGGGATGTTTCAAG+GGG 0.675676 5.2:-35098151 MS.gene95500:CDS
AGGGTTAGATGACAGCGAGG+AGG 0.678229 5.2:-35097866 MS.gene95500:CDS
AGTGATCAATCATATTCACA+TGG 0.693931 5.2:-35098261 MS.gene95500:CDS
GATGTGGACACAATAATACA+GGG 0.698577 5.2:-35098164 MS.gene95500:CDS
ATGTTGTCAATGCTGCACGT+CGG 0.718840 5.2:-35098608 MS.gene95500:CDS
TCAACTGTATATGTCAATGG+AGG 0.730272 5.2:-35098531 MS.gene95500:CDS
TGAAGGGTTAGATGACAGCG+AGG 0.736906 5.2:-35097869 MS.gene95500:CDS
AAATGAAACAGAATGACCGG+TGG 0.751744 5.2:+35098203 None:intergenic
ACCGTCACCAGAAACCATAG+CGG 0.784680 5.2:+35097984 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGAAATAATGAAACAAA+AGG + chr5.2:35097529-35097548 None:intergenic 15.0%
!! AAATTAGTATAAACATGAGA+CGG + chr5.2:35098452-35098471 None:intergenic 20.0%
!! AACAAAAGAATAGAATTTGA+AGG - chr5.2:35098365-35098384 MS.gene95500:CDS 20.0%
!! ACAAAAGAATAGAATTTGAA+GGG - chr5.2:35098366-35098385 MS.gene95500:CDS 20.0%
!!! GATTTTGAAGGATTAAATAT+AGG + chr5.2:35097870-35097889 None:intergenic 20.0%
!!! TGTTTATACTAATTTGGAAT+CGG - chr5.2:35098457-35098476 MS.gene95500:CDS 20.0%
! AAAGAAAAGAAGGGTAATAA+AGG + chr5.2:35097505-35097524 None:intergenic 25.0%
! AACAAAAGGAAAAGAAAAGA+AGG + chr5.2:35097515-35097534 None:intergenic 25.0%
! AAGAAAAGAAGGGTAATAAA+GGG + chr5.2:35097504-35097523 None:intergenic 25.0%
! ACAAAAGGAAAAGAAAAGAA+GGG + chr5.2:35097514-35097533 None:intergenic 25.0%
! ACAACACCATATACATTAAA+AGG + chr5.2:35097780-35097799 None:intergenic 25.0%
! GGAATCTTGAAATAAATGAT+TGG + chr5.2:35097682-35097701 None:intergenic 25.0%
! GTCTCATGTTTATACTAATT+TGG - chr5.2:35098451-35098470 MS.gene95500:CDS 25.0%
! TCATTTCCTAAAACTGTTAT+CGG - chr5.2:35098065-35098084 MS.gene95500:CDS 25.0%
! TTAAAATGTGCAGTGATTAT+AGG + chr5.2:35098575-35098594 None:intergenic 25.0%
!! TGAGTATTTGATGACTTATT+CGG - chr5.2:35097743-35097762 MS.gene95500:CDS 25.0%
!!! TTGTAACTTGATGATTTTGA+AGG + chr5.2:35097882-35097901 None:intergenic 25.0%
AATAATACAGGGATGTTTCA+AGG - chr5.2:35098098-35098117 MS.gene95500:CDS 30.0%
AATTGTGTTGTAAGAGACAT+AGG + chr5.2:35098158-35098177 None:intergenic 30.0%
ACACAATTGGAATCTTCAAT+TGG - chr5.2:35098170-35098189 MS.gene95500:CDS 30.0%
ACACCATATACATTAAAAGG+AGG + chr5.2:35097777-35097796 None:intergenic 30.0%
AGTGATCAATCATATTCACA+TGG - chr5.2:35097990-35098009 MS.gene95500:CDS 30.0%
ATAATACAGGGATGTTTCAA+GGG - chr5.2:35098099-35098118 MS.gene95500:CDS 30.0%
ATACAGGTAGTGACATTATT+TGG - chr5.2:35097802-35097821 MS.gene95500:CDS 30.0%
CAAAGGTTCAATAGTTGATT+AGG + chr5.2:35098521-35098540 None:intergenic 30.0%
CTGATGTGATACTATAACAA+AGG + chr5.2:35098538-35098557 None:intergenic 30.0%
GAATCAACTGTATATGTCAA+TGG - chr5.2:35097717-35097736 MS.gene95500:CDS 30.0%
TATGTCTCTTACAACACAAT+TGG - chr5.2:35098157-35098176 MS.gene95500:CDS 30.0%
TGAAGGATTAAATATAGGAG+TGG + chr5.2:35097865-35097884 None:intergenic 30.0%
! ACACCTCCTTTTAATGTATA+TGG - chr5.2:35097771-35097790 MS.gene95500:CDS 30.0%
! TATTTGATGACTTATTCGGT+TGG - chr5.2:35097747-35097766 MS.gene95500:CDS 30.0%
! TTATACTAATTTGGAATCGG+CGG - chr5.2:35098460-35098479 MS.gene95500:CDS 30.0%
! TTGACATATACAGTTGATTC+AGG + chr5.2:35097717-35097736 None:intergenic 30.0%
!! ACATTAGAAGCATTTAGTGT+TGG - chr5.2:35098341-35098360 MS.gene95500:CDS 30.0%
!!! TGAGAGTCTTTTTTCACAAA+AGG + chr5.2:35098305-35098324 None:intergenic 30.0%
AATTCCAAGACCAACTATAC+CGG + chr5.2:35098132-35098151 None:intergenic 35.0%
AGAGTTCTGTTCATTACAAG+AGG + chr5.2:35097949-35097968 None:intergenic 35.0%
AGGAAATGAAACAGAATGAC+CGG + chr5.2:35098054-35098073 None:intergenic 35.0%
CAATTGGAATCTTCAATTGG+TGG - chr5.2:35098173-35098192 MS.gene95500:CDS 35.0%
CTTGGCACAACTAAATTTGT+TGG - chr5.2:35098700-35098719 MS.gene95500:CDS 35.0%
GATGTGGACACAATAATACA+GGG - chr5.2:35098087-35098106 MS.gene95500:CDS 35.0%
GGTAACATCATACTTGATTC+AGG - chr5.2:35098407-35098426 MS.gene95500:CDS 35.0%
TAAATGATTGGCACGATTGA+TGG + chr5.2:35097670-35097689 None:intergenic 35.0%
TAAGCCTAGTGATTGTATCA+AGG - chr5.2:35098757-35098776 MS.gene95500:CDS 35.0%
TAATACAGGGATGTTTCAAG+GGG - chr5.2:35098100-35098119 MS.gene95500:CDS 35.0%
TACACCTTGATACAATCACT+AGG + chr5.2:35098764-35098783 None:intergenic 35.0%
TCAACTGTATATGTCAATGG+AGG - chr5.2:35097720-35097739 MS.gene95500:CDS 35.0%
TGACATAAGTTAGAAGAGCA+AGG + chr5.2:35097912-35097931 None:intergenic 35.0%
! AAGACGAGCAAACTCAATTT+TGG - chr5.2:35098239-35098258 MS.gene95500:CDS 35.0%
! ACATATACAGTTGATTCAGG+TGG + chr5.2:35097714-35097733 None:intergenic 35.0%
! GCTTTTACATCATCTCAAAC+TGG - chr5.2:35098662-35098681 MS.gene95500:CDS 35.0%
! TAGTGTTGAGACTCTTACAT+TGG - chr5.2:35098019-35098038 MS.gene95500:CDS 35.0%
!! ACATGAGCAAAGGTACTAAT+TGG + chr5.2:35098620-35098639 None:intergenic 35.0%
!! CATGAGCAAAGGTACTAATT+GGG + chr5.2:35098619-35098638 None:intergenic 35.0%
!! GCACAACTAAATTTGTTGGT+TGG - chr5.2:35098704-35098723 MS.gene95500:CDS 35.0%
!! GTATATGGTGTTGTTGATAC+AGG - chr5.2:35097786-35097805 MS.gene95500:CDS 35.0%
AAATGAAACAGAATGACCGG+TGG + chr5.2:35098051-35098070 None:intergenic 40.0%
CCTAAAACTGTTATCGGATG+TGG - chr5.2:35098071-35098090 MS.gene95500:CDS 40.0%
GGATGTGGACACAATAATAC+AGG - chr5.2:35098086-35098105 MS.gene95500:CDS 40.0%
TGCTTGTAGCATTGTTCACA+AGG + chr5.2:35097840-35097859 None:intergenic 40.0%
TGTGCCAAGTTTCCAAAGAT+GGG + chr5.2:35098689-35098708 None:intergenic 40.0%
TTGTGCCAAGTTTCCAAAGA+TGG + chr5.2:35098690-35098709 None:intergenic 40.0%
TTGTTCGAGTCAACCATCAA+CGG + chr5.2:35098221-35098240 None:intergenic 40.0%
! CCACATCCGATAACAGTTTT+AGG + chr5.2:35098074-35098093 None:intergenic 40.0%
! TTACTGTTTGTTGCCGTTGA+TGG - chr5.2:35098205-35098224 MS.gene95500:CDS 40.0%
! TTTTGTGCAGGTTTGTAGAG+TGG + chr5.2:35097612-35097631 None:intergenic 40.0%
!! GGTATAGTTGGTCTTGGAAT+TGG - chr5.2:35098131-35098150 MS.gene95500:CDS 40.0%
!! GTGGTGATTTTGAAGAATCG+CGG + chr5.2:35097593-35097612 None:intergenic 40.0%
!! TGATTCAGGTGGATTAGAGA+GGG + chr5.2:35097703-35097722 None:intergenic 40.0%
!! TTGATTCAGGTGGATTAGAG+AGG + chr5.2:35097704-35097723 None:intergenic 40.0%
!!! CACTGCACATTTTAAAGCTG+CGG - chr5.2:35098580-35098599 MS.gene95500:CDS 40.0%
!!! TGCTGGAATTTGTTTTGTGC+AGG + chr5.2:35097624-35097643 None:intergenic 40.0%
ACCATCAGCAACATGAGCAA+AGG + chr5.2:35098630-35098649 None:intergenic 45.0%
ACCTTTGCTCATGTTGCTGA+TGG - chr5.2:35098626-35098645 MS.gene95500:CDS 45.0%
ATGTTGTCAATGCTGCACGT+CGG - chr5.2:35097643-35097662 MS.gene95500:CDS 45.0%
ATGTTTCAAGGGGCAACTTC+CGG - chr5.2:35098110-35098129 MS.gene95500:CDS 45.0%
CTTACATTGGATTCCACCAC+CGG - chr5.2:35098032-35098051 MS.gene95500:CDS 45.0%
TCTCAAACTGGTCCCATCTT+TGG - chr5.2:35098674-35098693 MS.gene95500:CDS 45.0%
! ACTTCCGGTATAGTTGGTCT+TGG - chr5.2:35098125-35098144 MS.gene95500:CDS 45.0%
! TACTAATTTGGAATCGGCGG+TGG - chr5.2:35098463-35098482 MS.gene95500:CDS 45.0%
! TGGTCCCATCTTTGGAAACT+TGG - chr5.2:35098682-35098701 MS.gene95500:CDS 45.0%
!! CAATTTTGGAGATGCCGCTA+TGG - chr5.2:35098253-35098272 MS.gene95500:CDS 45.0%
!! GTGATTTTGAAGAATCGCGG+TGG + chr5.2:35097590-35097609 None:intergenic 45.0%
ACCGTCACCAGAAACCATAG+CGG + chr5.2:35098270-35098289 None:intergenic 50.0%
TGAAACAGAATGACCGGTGG+TGG + chr5.2:35098048-35098067 None:intergenic 50.0%
TGAAGGGTTAGATGACAGCG+AGG - chr5.2:35098382-35098401 MS.gene95500:CDS 50.0%
! TGCTCATGTTGCTGATGGTG+TGG - chr5.2:35098631-35098650 MS.gene95500:CDS 50.0%
!! GTGCAGCATTGACAACATGC+TGG + chr5.2:35097641-35097660 None:intergenic 50.0%
AGGGTTAGATGACAGCGAGG+AGG - chr5.2:35098385-35098404 MS.gene95500:CDS 55.0%
GGAGATGCCGCTATGGTTTC+TGG - chr5.2:35098260-35098279 MS.gene95500:CDS 55.0%
GGGGCAACTTCCGGTATAGT+TGG - chr5.2:35098119-35098138 MS.gene95500:CDS 55.0%
GGGTTAGATGACAGCGAGGA+GGG - chr5.2:35098386-35098405 MS.gene95500:CDS 55.0%
!! GCCGCTATGGTTTCTGGTGA+CGG - chr5.2:35098266-35098285 MS.gene95500:CDS 55.0%
GGAATCGGCGGTGGCACAAT+TGG - chr5.2:35098472-35098491 MS.gene95500:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 35097489 35098784 35097489 ID=MS.gene95500
chr5.2 mRNA 35097489 35098784 35097489 ID=MS.gene95500.t1;Parent=MS.gene95500
chr5.2 exon 35097489 35098784 35097489 ID=MS.gene95500.t1.exon1;Parent=MS.gene95500.t1
chr5.2 CDS 35097489 35098784 35097489 ID=cds.MS.gene95500.t1;Parent=MS.gene95500.t1
Gene Sequence

>MS.gene95500

ATGAATACTCGTCCCTTTATTACCCTTCTTTTCTTTTCCTTTTGTTTCATTATTTCTTTTTCTCATTCTCTACGCAATAGTTTTAGTTTTGAACTCATCCACCGCGATTCTTCAAAATCACCACTCTACAAACCTGCACAAAACAAATTCCAGCATGTTGTCAATGCTGCACGTCGGTCCATCAATCGTGCCAATCATTTATTTCAAGATTCCCTCTCTAATCCACCTGAATCAACTGTATATGTCAATGGAGGTGAGTATTTGATGACTTATTCGGTTGGTACACCTCCTTTTAATGTATATGGTGTTGTTGATACAGGTAGTGACATTATTTGGCTTCAATGTAAGCCTTGTGAACAATGCTACAAGCAAACCACTCCTATATTTAATCCTTCAAAATCATCAAGTTACAAAAATATTCCTTGCTCTTCTAACTTATGTCAATCTGTGAGATATACCTCTTGTAATGAACAGAACTCTTGTGAATATACTATTAACTTTAGTGATCAATCATATTCACATGGAGAACTTAGTGTTGAGACTCTTACATTGGATTCCACCACCGGTCATTCTGTTTCATTTCCTAAAACTGTTATCGGATGTGGACACAATAATACAGGGATGTTTCAAGGGGCAACTTCCGGTATAGTTGGTCTTGGAATTGGACCTATGTCTCTTACAACACAATTGGAATCTTCAATTGGTGGTAAATTTTCTTACTGTTTGTTGCCGTTGATGGTTGACTCGAACAAGACGAGCAAACTCAATTTTGGAGATGCCGCTATGGTTTCTGGTGACGGTGCTGTGTCAACTCCTTTTGTGAAAAAAGACTCTCAAGTTTTCTACTATTTGACATTAGAAGCATTTAGTGTTGGAAACAAAAGAATAGAATTTGAAGGGTTAGATGACAGCGAGGAGGGTAACATCATACTTGATTCAGGTACAACATTGACTCTTTTACCGTCTCATGTTTATACTAATTTGGAATCGGCGGTGGCACAATTGGTGAAACTAGATCGTGTTGATGATCCTAATCAACTATTGAACCTTTGTTATAGTATCACATCAGATCAATATGATTTTCCTATAATCACTGCACATTTTAAAGCTGCGGATATTAAGTTGAACCCAATTAGTACCTTTGCTCATGTTGCTGATGGTGTGGTTTGCTTAGCTTTTACATCATCTCAAACTGGTCCCATCTTTGGAAACTTGGCACAACTAAATTTGTTGGTTGGTTATGATCTTCAACAAAATATTGTGTCGTTTAAGCCTAGTGATTGTATCAAGGTGTAA
Protein sequence

>MS.gene95500.t1

MNTRPFITLLFFSFCFIISFSHSLRNSFSFELIHRDSSKSPLYKPAQNKFQHVVNAARRSINRANHLFQDSLSNPPESTVYVNGGEYLMTYSVGTPPFNVYGVVDTGSDIIWLQCKPCEQCYKQTTPIFNPSKSSSYKNIPCSSNLCQSVRYTSCNEQNSCEYTINFSDQSYSHGELSVETLTLDSTTGHSVSFPKTVIGCGHNNTGMFQGATSGIVGLGIGPMSLTTQLESSIGGKFSYCLLPLMVDSNKTSKLNFGDAAMVSGDGAVSTPFVKKDSQVFYYLTLEAFSVGNKRIEFEGLDDSEEGNIILDSGTTLTLLPSHVYTNLESAVAQLVKLDRVDDPNQLLNLCYSITSDQYDFPIITAHFKAADIKLNPISTFAHVADGVVCLAFTSSQTGPIFGNLAQLNLLVGYDLQQNIVSFKPSDCIKV