AlfalfaGEDB | MS.gene95566

M. sativa cultivar XinJiangDaYe / MS.gene95566

KEGG: K11251
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF00125.27 PF16211.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95566.t1	XP_004495152.1	94.8	134	7	0	1	134	1	134	1.00E-60	242.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95566.t1	Q84MP7	85.4	137	17	1	1	134	1	137	2.2e-56	219.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95566.t1	G7ICP3	94.8	134	7	0	1	134	1	134	7.2e-61	242.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene95566	MS.gene76254	PPI
MS.gene065078	MS.gene95566	PPI
MS.gene029348	MS.gene95566	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95566.t1	MTR_1g071040	94.776	134	7	0	1	134	1	134	1.14e-81	236
MS.gene95566.t1	MTR_1g114050	87.313	134	14	1	1	134	1	131	6.18e-79	229
MS.gene95566.t1	MTR_1g011750	82.836	134	23	0	1	134	1	134	1.02e-75	221
MS.gene95566.t1	MTR_1g011850	82.836	134	23	0	1	134	1	134	1.02e-75	221
MS.gene95566.t1	MTR_4g048490	67.164	134	15	1	1	134	1	105	1.25e-45	144
MS.gene95566.t1	MTR_7g027610	65.574	122	16	2	13	134	3	98	1.39e-42	136
MS.gene95566.t1	MTR_2g082510	64.815	108	36	2	24	130	14	120	1.29e-38	127
MS.gene95566.t1	MTR_2g082220	64.815	108	36	2	24	130	14	120	1.48e-38	127
MS.gene95566.t1	MTR_7g108320	62.617	107	38	2	25	130	21	126	6.87e-38	125
MS.gene95566.t1	MTR_7g114060	62.617	107	38	2	25	130	18	123	1.25e-37	125
MS.gene95566.t1	MTR_7g114040	62.617	107	38	2	25	130	18	123	1.25e-37	125
MS.gene95566.t1	MTR_7g093910	55.118	127	50	3	10	130	1	126	2.74e-37	124
MS.gene95566.t1	MTR_2g096610	60.550	109	41	2	23	130	19	126	1.91e-35	119
MS.gene95566.t1	MTR_7g113160	55.752	113	48	2	19	130	19	130	3.03e-35	119
MS.gene95566.t1	MTR_4g063280	60.550	109	41	2	23	130	15	122	8.40e-35	118
MS.gene95566.t1	MTR_4g071150	62.500	104	37	2	28	130	26	128	4.87e-34	116
MS.gene95566.t1	MTR_4g064967	60.952	105	39	2	27	130	25	128	6.80e-34	115
MS.gene95566.t1	MTR_2g096570	60.952	105	39	2	27	130	24	127	9.12e-34	115
MS.gene95566.t1	MTR_4g064005	61.538	104	38	2	28	130	26	128	1.06e-33	115
MS.gene95566.t1	MTR_8g086640	60.952	105	39	2	27	130	25	128	1.52e-33	115
MS.gene95566.t1	MTR_4g063410	60.000	105	40	2	27	130	25	128	3.56e-33	114
MS.gene95566.t1	MTR_0653s0030	58.333	108	20	1	5	112	15	97	7.38e-33	113
MS.gene95566.t1	MTR_7g114050	50.000	130	48	6	9	133	1	118	8.46e-27	97.4
MS.gene95566.t1	MTR_4g063300	62.687	67	24	1	55	120	17	83	6.48e-20	79.3
MS.gene95566.t1	MTR_6g005150	45.283	106	47	4	27	130	27	123	1.48e-18	76.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95566.t1	AT3G54560	87.500	136	15	1	1	134	1	136	9.46e-74	216
MS.gene95566.t1	AT3G54560	87.500	136	15	1	1	134	1	136	9.46e-74	216
MS.gene95566.t1	AT2G38810	85.294	136	18	1	1	134	1	136	2.54e-72	213
MS.gene95566.t1	AT2G38810	85.294	136	18	1	1	134	1	136	2.54e-72	213
MS.gene95566.t1	AT2G38810	85.294	136	18	1	1	134	1	136	2.54e-72	213
MS.gene95566.t1	AT2G38810	85.294	136	18	1	1	134	1	136	2.54e-72	213
MS.gene95566.t1	AT1G52740	79.104	134	28	0	1	134	1	134	1.50e-67	200
MS.gene95566.t1	AT4G13570	74.725	91	23	0	34	124	28	118	1.52e-44	142
MS.gene95566.t1	AT4G13570	75.556	90	22	0	35	124	35	124	3.06e-44	141
MS.gene95566.t1	AT3G20670	64.815	108	36	2	24	130	14	120	6.87e-39	128
MS.gene95566.t1	AT4G27230	64.815	108	36	2	24	130	14	120	1.13e-38	127
MS.gene95566.t1	AT4G27230	64.815	108	36	2	24	130	14	120	1.13e-38	127
MS.gene95566.t1	AT5G54640	64.815	108	36	2	24	130	14	120	1.23e-38	127
MS.gene95566.t1	AT1G51060	63.889	108	37	2	24	130	14	120	1.88e-38	127
MS.gene95566.t1	AT5G02560	62.385	109	39	2	23	130	21	128	7.48e-38	126
MS.gene95566.t1	AT1G08880	62.162	111	39	3	25	133	21	130	7.60e-38	125
MS.gene95566.t1	AT1G54690	62.162	111	39	3	25	133	21	130	1.03e-37	125
MS.gene95566.t1	AT5G27670	60.177	113	43	2	19	130	18	129	4.36e-36	121
MS.gene95566.t1	AT5G59870	59.633	109	42	2	23	130	21	128	2.74e-34	117
MS.gene95566.t1	AT5G02560	51.128	133	39	3	23	130	21	152	1.94e-32	113

Find 40 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCATTTGCAGCTGGCTATTA+GGG	0.226611	4.3:+50642254	MS.gene95566:CDS
GGCATTTGCAGCTGGCTATT+AGG	0.249205	4.3:+50642253	MS.gene95566:CDS
AGCAACAGCTGCAGTTTATC+TGG	0.338549	4.3:+50642143	MS.gene95566:CDS
AGTTTATCTGGCATCAATTC+TGG	0.339438	4.3:+50642155	MS.gene95566:CDS
GGATCCTACCCACAGGAAAC+TGG	0.357207	4.3:-50642077	None:intergenic
CAGCAATTGTTCCCTTGATA+AGG	0.371937	4.3:-50642296	None:intergenic
CTTATCAAGGGAACAATTGC+TGG	0.372156	4.3:+50642297	MS.gene95566:CDS
AAGCAAAGAGTCCAAGCTAA+TGG	0.419783	4.3:+50642111	MS.gene95566:CDS
CTCCCACACGTCCATTAGCT+TGG	0.422586	4.3:-50642122	None:intergenic
AGCAATTGTTCCCTTGATAA+GGG	0.441041	4.3:-50642295	None:intergenic
AAGTCCTTGATCAACAAAAC+TGG	0.444348	4.3:+50642345	MS.gene95566:CDS
CCAATGATTTGAAGGTGAAA+AGG	0.447327	4.3:+50642220	MS.gene95566:CDS
CTCTTTGCTTTAGTTGTCTG+TGG	0.458030	4.3:-50642098	None:intergenic
ACTTCTCGTTCTTCTCGTGC+TGG	0.468244	4.3:+50642051	MS.gene95566:CDS
GACTGCAGAAGTTCTTGAAC+TGG	0.482588	4.3:+50642185	MS.gene95566:CDS
CACACCAAGGCATTTGCAGC+TGG	0.498611	4.3:+50642245	MS.gene95566:CDS
GGAACTTGATACCCTTATCA+AGG	0.512125	4.3:+50642284	MS.gene95566:CDS
GCAGAAGTTCTTGAACTGGC+TGG	0.518730	4.3:+50642189	MS.gene95566:CDS
GATTAAGCAATGGCAGGAAA+AGG	0.523608	4.3:+50641964	None:intergenic
AAATGCAACCAATGATTTGA+AGG	0.529114	4.3:+50642212	MS.gene95566:CDS
TGCTGGAATCCAGTTTCCTG+TGG	0.532053	4.3:+50642068	MS.gene95566:CDS
ATCAAGGACTTGTGAATGCT+AGG	0.547647	4.3:-50642333	None:intergenic
ATAGCCAGCTGCAAATGCCT+TGG	0.558142	4.3:-50642249	None:intergenic
CAAGGTGATTAAGCAATGGC+AGG	0.563211	4.3:+50641958	None:intergenic
AATGGCAGGAAAAGGAGAAG+AGG	0.580632	4.3:+50641972	None:intergenic
GTCCAAGCTAATGGACGTGT+GGG	0.594226	4.3:+50642120	MS.gene95566:CDS
GAATCCAGTTTCCTGTGGGT+AGG	0.598381	4.3:+50642073	MS.gene95566:CDS
ATGGCAGGAAAAGGAGAAGA+GGG	0.598808	4.3:+50641973	MS.gene95566:CDS
AGTCCAAGCTAATGGACGTG+TGG	0.600498	4.3:+50642119	MS.gene95566:CDS
AGCACGAGAAGAACGAGAAG+TGG	0.609010	4.3:-50642049	None:intergenic
CATTTGCAGCTGGCTATTAG+GGG	0.616027	4.3:+50642255	MS.gene95566:CDS
GCTGGCTATTAGGGGAGATG+AGG	0.618047	4.3:+50642263	MS.gene95566:CDS
GCTGGAATCCAGTTTCCTGT+GGG	0.620170	4.3:+50642069	MS.gene95566:CDS
AAGGGAACAATTGCTGGTGG+TGG	0.621502	4.3:+50642303	MS.gene95566:CDS
GAACTTGATACCCTTATCAA+GGG	0.630061	4.3:+50642285	MS.gene95566:CDS
GCACGAGAAGAACGAGAAGT+GGG	0.642443	4.3:-50642048	None:intergenic
TGTCTGTGGATCCTACCCAC+AGG	0.650154	4.3:-50642084	None:intergenic
ATCAAGGGAACAATTGCTGG+TGG	0.659902	4.3:+50642300	MS.gene95566:CDS
AGGTGAAAAGGATCACACCA+AGG	0.731656	4.3:+50642232	MS.gene95566:CDS
CGTTGCAGCGAAAATCACTG+CGG	0.858258	4.3:+50641999	MS.gene95566:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAATGCAACCAATGATTTGA+AGG	+	chr4.3:50642212-50642231	MS.gene95566:CDS	30.0%
	AAGTCCTTGATCAACAAAAC+TGG	+	chr4.3:50642345-50642364	MS.gene95566:CDS	35.0%
	AGCAATTGTTCCCTTGATAA+GGG	-	chr4.3:50642298-50642317	None:intergenic	35.0%
	AGTTTATCTGGCATCAATTC+TGG	+	chr4.3:50642155-50642174	MS.gene95566:CDS	35.0%
	CCAATGATTTGAAGGTGAAA+AGG	+	chr4.3:50642220-50642239	MS.gene95566:CDS	35.0%
	GAACTTGATACCCTTATCAA+GGG	+	chr4.3:50642285-50642304	MS.gene95566:CDS	35.0%
!	CCTTTTCACCTTCAAATCAT+TGG	-	chr4.3:50642223-50642242	None:intergenic	35.0%
!!!	TTTGCCAGTTTTGTTGATCA+AGG	-	chr4.3:50642352-50642371	None:intergenic	35.0%
	AAGCAAAGAGTCCAAGCTAA+TGG	+	chr4.3:50642111-50642130	MS.gene95566:CDS	40.0%
	ATCAAGGACTTGTGAATGCT+AGG	-	chr4.3:50642336-50642355	None:intergenic	40.0%
	CAGCAATTGTTCCCTTGATA+AGG	-	chr4.3:50642299-50642318	None:intergenic	40.0%
	CTCTTTGCTTTAGTTGTCTG+TGG	-	chr4.3:50642101-50642120	None:intergenic	40.0%
	CTTATCAAGGGAACAATTGC+TGG	+	chr4.3:50642297-50642316	MS.gene95566:CDS	40.0%
	GGAACTTGATACCCTTATCA+AGG	+	chr4.3:50642284-50642303	MS.gene95566:CDS	40.0%
	AGCAACAGCTGCAGTTTATC+TGG	+	chr4.3:50642143-50642162	MS.gene95566:CDS	45.0%
	AGGTGAAAAGGATCACACCA+AGG	+	chr4.3:50642232-50642251	MS.gene95566:CDS	45.0%
	ATCAAGGGAACAATTGCTGG+TGG	+	chr4.3:50642300-50642319	MS.gene95566:CDS	45.0%
	ATGGCAGGAAAAGGAGAAGA+GGG	+	chr4.3:50641973-50641992	MS.gene95566:CDS	45.0%
	CATTTGCAGCTGGCTATTAG+GGG	+	chr4.3:50642255-50642274	MS.gene95566:CDS	45.0%
	GACTGCAGAAGTTCTTGAAC+TGG	+	chr4.3:50642185-50642204	MS.gene95566:CDS	45.0%
	GCATTTGCAGCTGGCTATTA+GGG	+	chr4.3:50642254-50642273	MS.gene95566:CDS	45.0%
	AAGGGAACAATTGCTGGTGG+TGG	+	chr4.3:50642303-50642322	MS.gene95566:CDS	50.0%
	ACTTCTCGTTCTTCTCGTGC+TGG	+	chr4.3:50642051-50642070	MS.gene95566:CDS	50.0%
	AGCACGAGAAGAACGAGAAG+TGG	-	chr4.3:50642052-50642071	None:intergenic	50.0%
	ATAGCCAGCTGCAAATGCCT+TGG	-	chr4.3:50642252-50642271	None:intergenic	50.0%
	CGTTGCAGCGAAAATCACTG+CGG	+	chr4.3:50641999-50642018	MS.gene95566:CDS	50.0%
	GAATCCAGTTTCCTGTGGGT+AGG	+	chr4.3:50642073-50642092	MS.gene95566:CDS	50.0%
	GCACGAGAAGAACGAGAAGT+GGG	-	chr4.3:50642051-50642070	None:intergenic	50.0%
	GCAGAAGTTCTTGAACTGGC+TGG	+	chr4.3:50642189-50642208	MS.gene95566:CDS	50.0%
	GCTGGAATCCAGTTTCCTGT+GGG	+	chr4.3:50642069-50642088	MS.gene95566:CDS	50.0%
	GGCATTTGCAGCTGGCTATT+AGG	+	chr4.3:50642253-50642272	MS.gene95566:CDS	50.0%
	TGCTGGAATCCAGTTTCCTG+TGG	+	chr4.3:50642068-50642087	MS.gene95566:CDS	50.0%
!	AGTCCAAGCTAATGGACGTG+TGG	+	chr4.3:50642119-50642138	MS.gene95566:CDS	50.0%
!	GTCCAAGCTAATGGACGTGT+GGG	+	chr4.3:50642120-50642139	MS.gene95566:CDS	50.0%
	CTCCCACACGTCCATTAGCT+TGG	-	chr4.3:50642125-50642144	None:intergenic	55.0%
	GCTGGCTATTAGGGGAGATG+AGG	+	chr4.3:50642263-50642282	MS.gene95566:CDS	55.0%
	GGATCCTACCCACAGGAAAC+TGG	-	chr4.3:50642080-50642099	None:intergenic	55.0%
	TGTCTGTGGATCCTACCCAC+AGG	-	chr4.3:50642087-50642106	None:intergenic	55.0%
!	CACACCAAGGCATTTGCAGC+TGG	+	chr4.3:50642245-50642264	MS.gene95566:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	50641973	50642377	50641973	ID=MS.gene95566
chr4.3	mRNA	50641973	50642377	50641973	ID=MS.gene95566.t1;Parent=MS.gene95566
chr4.3	exon	50641973	50642377	50641973	ID=MS.gene95566.t1.exon1;Parent=MS.gene95566.t1
chr4.3	CDS	50641973	50642377	50641973	ID=cds.MS.gene95566.t1;Parent=MS.gene95566.t1

Gene Sequence

>MS.gene95566

ATGGCAGGAAAAGGAGAAGAGGGACTCGTTGCAGCGAAAATCACTGCGGCTAACAAAGACAAAGACAAGAAGAAACCCACTTCTCGTTCTTCTCGTGCTGGAATCCAGTTTCCTGTGGGTAGGATCCACAGACAACTAAAGCAAAGAGTCCAAGCTAATGGACGTGTGGGAGCAACAGCTGCAGTTTATCTGGCATCAATTCTGGAGTATCTGACTGCAGAAGTTCTTGAACTGGCTGGAAATGCAACCAATGATTTGAAGGTGAAAAGGATCACACCAAGGCATTTGCAGCTGGCTATTAGGGGAGATGAGGAACTTGATACCCTTATCAAGGGAACAATTGCTGGTGGTGGTGTCATTCCTAGCATTCACAAGTCCTTGATCAACAAAACTGGCAAAGAATGA

Protein sequence

>MS.gene95566.t1

MAGKGEEGLVAAKITAANKDKDKKKPTSRSSRAGIQFPVGRIHRQLKQRVQANGRVGATAAVYLASILEYLTAEVLELAGNATNDLKVKRITPRHLQLAIRGDEELDTLIKGTIAGGGVIPSIHKSLINKTGKE