AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96314


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96314.t1 MTR_4g127290 98.489 331 5 0 1 331 1 331 0.0 666
MS.gene96314.t1 MTR_2g038200 76.136 176 34 4 1 172 1 172 4.05e-66 224
MS.gene96314.t1 MTR_2g038200 69.880 83 23 2 247 329 399 479 6.12e-26 108
MS.gene96314.t1 MTR_1g057150 63.102 187 49 5 1 177 1 177 1.31e-65 221
MS.gene96314.t1 MTR_1g090640 63.636 176 49 5 1 170 1 167 3.49e-60 207
MS.gene96314.t1 MTR_4g129230 63.068 176 56 3 1 174 1 169 4.49e-55 193
MS.gene96314.t1 MTR_3g464150 44.767 172 80 4 9 166 9 179 4.34e-26 109
MS.gene96314.t1 MTR_5g071060 38.559 236 128 7 9 230 9 241 2.19e-25 107
MS.gene96314.t1 MTR_5g071060 38.559 236 128 7 9 230 9 241 2.23e-25 107
MS.gene96314.t1 MTR_3g070850 42.604 169 83 4 9 166 9 174 6.15e-23 100
MS.gene96314.t1 MTR_1183s0010 50.000 92 41 2 84 173 12 100 1.81e-16 79.0
MS.gene96314.t1 MTR_1384s0010 39.394 132 45 7 1 125 1 104 4.35e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96314.t1 AT5G57130 61.905 231 60 5 1 208 1 226 1.17e-75 251
MS.gene96314.t1 AT4G29920 58.506 241 73 7 1 218 1 237 2.87e-64 219
MS.gene96314.t1 AT4G29920 58.506 241 73 7 1 218 1 237 2.97e-64 219
MS.gene96314.t1 AT3G52490 63.687 179 50 4 1 174 1 169 7.38e-62 211
MS.gene96314.t1 AT5G57710 58.291 199 64 5 1 189 1 190 6.71e-55 193
MS.gene96314.t1 AT4G30350 55.392 204 71 5 1 192 1 196 1.06e-48 175
MS.gene96314.t1 AT1G07200 44.379 169 80 3 9 166 9 174 2.52e-30 122
MS.gene96314.t1 AT2G29970 44.311 167 83 3 9 166 9 174 3.64e-30 121
MS.gene96314.t1 AT2G40130 45.455 165 80 3 9 166 9 170 4.05e-30 121
MS.gene96314.t1 AT2G40130 45.455 165 80 3 9 166 9 170 4.41e-30 121

Find 56 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAGAAAACATTGCCTTTC+TGG 0.197917 8.3:+3750274 MS.gene96314:CDS
GTTTCAATGTAGCTCTTAAT+AGG 0.268715 8.3:+3748953 MS.gene96314:CDS
TTGCTGCTGCTGCTGTTCTA+TGG 0.269718 8.3:-3749076 None:intergenic
GCTTGACCCTTCATTCTTCC+AGG 0.278647 8.3:+3749469 MS.gene96314:CDS
TTTAAATGTTATAATAGTTC+TGG 0.304204 8.3:+3749255 MS.gene96314:CDS
ATTCTCTTGTGTTGGCTAGA+AGG 0.358333 8.3:+3748782 MS.gene96314:CDS
TTTGAAGGAACTTAGTCTTA+AGG 0.360363 8.3:-3748854 None:intergenic
TTTCAGACAGAACAATTTCA+TGG 0.360555 8.3:+3749350 MS.gene96314:CDS
AAAGCCCTTTAGCAGTAATA+AGG 0.361117 8.3:+3750170 MS.gene96314:CDS
TGGCACAGAAGAACAAAATA+AGG 0.368675 8.3:+3750308 MS.gene96314:CDS
TAAGACTAAGTTCCTTCAAA+AGG 0.375696 8.3:+3748857 MS.gene96314:CDS
CCTTTCTGGCTTCAGTCTCA+TGG 0.390835 8.3:+3750288 MS.gene96314:CDS
TTGTTTCCAGCATATGAGAT+AGG 0.393515 8.3:-3750149 None:intergenic
TTGATGATCCTAGTGTTAGT+AGG 0.397262 8.3:+3749154 MS.gene96314:CDS
CTGTGATTCTCACTAGTTGA+AGG 0.411339 8.3:-3749306 None:intergenic
AGTAGGGTTATGAGAGAAGC+TGG 0.424536 8.3:+3749171 MS.gene96314:CDS
TGCACCTTATTACTGCTAAA+GGG 0.429521 8.3:-3750174 None:intergenic
GTGGATGTTGTGAAGTTTGA+TGG 0.431778 8.3:-3748906 None:intergenic
CAATGCAGCAATGAGAGCAT+TGG 0.451420 8.3:-3749022 None:intergenic
TTTGAAGCATTCTCTTGTGT+TGG 0.454108 8.3:+3748774 MS.gene96314:CDS
AATATTACCTGGAAGAATGA+AGG 0.464963 8.3:-3749476 None:intergenic
ATGCACCTTATTACTGCTAA+AGG 0.466662 8.3:-3750175 None:intergenic
GCAGCAGCAGCAACAGCAAA+CGG 0.470948 8.3:+3749086 MS.gene96314:CDS
AGATGCTCATCTTGTAATCA+AGG 0.479818 8.3:-3750119 None:intergenic
AAGACTAAGTTCCTTCAAAA+GGG 0.480191 8.3:+3748858 MS.gene96314:CDS
ATCAAAGGAGAAGGTGTTGT+TGG 0.481111 8.3:-3748979 None:intergenic
ACTGAGTTGACGAGTACATC+AGG 0.485948 8.3:+3749438 MS.gene96314:CDS
TGATGATCCTAGTGTTAGTA+GGG 0.489344 8.3:+3749155 MS.gene96314:CDS
GAAGGCTGAGAGTGAATCAA+AGG 0.518124 8.3:-3748994 None:intergenic
TGTTGTGAAGTTTGATGGTG+TGG 0.518229 8.3:-3748901 None:intergenic
TCAGAACCTATCTCATATGC+TGG 0.523908 8.3:+3750143 MS.gene96314:CDS
CCATGAGACTGAAGCCAGAA+AGG 0.527419 8.3:-3750288 None:intergenic
ACGAGTACATCAGGTGGACT+TGG 0.541210 8.3:+3749447 MS.gene96314:CDS
ATATATCAAGTGATGCGCTC+AGG 0.544488 8.3:+3748706 None:intergenic
TGAAGCAGCCTCTGCTGTGA+GGG 0.545563 8.3:-3748749 None:intergenic
GCTCAATTTCTCAGACCTGC+AGG 0.549147 8.3:+3750249 MS.gene96314:CDS
AAATGTTATAATAGTTCTGG+TGG 0.549816 8.3:+3749258 MS.gene96314:CDS
ATATTACCTGGAAGAATGAA+GGG 0.561317 8.3:-3749475 None:intergenic
TGTTCTGTCTGAAACTGCTA+AGG 0.561994 8.3:-3749341 None:intergenic
GCACAAGCTCATCAAAGAAG+AGG 0.562580 8.3:+3749051 MS.gene96314:CDS
TCACTAGTTGAAGGTGAACA+TGG 0.565455 8.3:-3749297 None:intergenic
TGAGAGTGAATCAAAGGAGA+AGG 0.577096 8.3:-3748988 None:intergenic
CTCATAACCCTACTAACACT+AGG 0.579503 8.3:-3749162 None:intergenic
AAAGTGATGGCTACATGTAG+AGG 0.593224 8.3:-3748826 None:intergenic
TCAAGAGCTCTAGATTGAAG+TGG 0.600534 8.3:-3748925 None:intergenic
TCTTAAGGTAAGTAAAGTGA+TGG 0.602666 8.3:-3748839 None:intergenic
CTTGTGTTGGCTAGAAGGAG+AGG 0.606431 8.3:+3748787 MS.gene96314:CDS
CTGAAGCAGCCTCTGCTGTG+AGG 0.607118 8.3:-3748750 None:intergenic
ATGAGAGCATTGGAAAGTGA+AGG 0.611410 8.3:-3749012 None:intergenic
TAGCAGTAATAAGGTGCATG+AGG 0.621940 8.3:+3750179 MS.gene96314:CDS
TATCAAGTGATGCGCTCAGG+AGG 0.632949 8.3:+3748709 None:intergenic
TGTTATAATAGTTCTGGTGG+TGG 0.635592 8.3:+3749261 MS.gene96314:CDS
GCAGCAGACCCTCACAGCAG+AGG 0.647393 8.3:+3748741 MS.gene96314:CDS
TAGAAGGAGAGGTCATCCAC+AGG 0.649362 8.3:+3748798 MS.gene96314:CDS
ACATGTAGAGGAGTGACCTG+TGG 0.684775 8.3:-3748814 None:intergenic
GAGTTGACGAGTACATCAGG+TGG 0.710146 8.3:+3749441 MS.gene96314:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAATGTTATAATAGTTC+TGG + chr8.3:3749255-3749274 MS.gene96314:CDS 15.0%
!!! TTCTTAATTTGTTTGATTTA+AGG + chr8.3:3749620-3749639 MS.gene96314:intron 15.0%
!! AAGCAAAATAAAATATTACC+TGG - chr8.3:3749490-3749509 None:intergenic 20.0%
!! CATTTAAAAACAGAATTAGA+AGG - chr8.3:3749243-3749262 None:intergenic 20.0%
!! CCATAATTAAGCAAATAAAT+AGG - chr8.3:3750066-3750085 None:intergenic 20.0%
!! CCTATTTATTTGCTTAATTA+TGG + chr8.3:3750063-3750082 MS.gene96314:intron 20.0%
!!! TGTTTGATTTAAGGTATAAA+AGG + chr8.3:3749629-3749648 MS.gene96314:intron 20.0%
!!! TTATGTTTACCTTTCTTTTT+TGG - chr8.3:3749680-3749699 None:intergenic 20.0%
! AAAGACTACACTTTATCTAA+AGG + chr8.3:3749949-3749968 MS.gene96314:intron 25.0%
! ACACATGTTATGTTACTAAA+AGG - chr8.3:3749601-3749620 None:intergenic 25.0%
! TAAATGCAACCAAAAAAGAA+AGG + chr8.3:3749668-3749687 MS.gene96314:intron 25.0%
! TATAACACTTAGTACATGTT+TGG + chr8.3:3749520-3749539 MS.gene96314:intron 25.0%
!!! AAATGTTATAATAGTTCTGG+TGG + chr8.3:3749258-3749277 MS.gene96314:CDS 25.0%
!!! TCAGTCAAATTATTTTGAGT+TGG + chr8.3:3749918-3749937 MS.gene96314:intron 25.0%
!!! TTGAGCTTCTTTTTCATAAT+TGG - chr8.3:3750235-3750254 None:intergenic 25.0%
AAGACTAAGTTCCTTCAAAA+GGG + chr8.3:3748858-3748877 MS.gene96314:CDS 30.0%
AATATTACCTGGAAGAATGA+AGG - chr8.3:3749479-3749498 None:intergenic 30.0%
ATATTACCTGGAAGAATGAA+GGG - chr8.3:3749478-3749497 None:intergenic 30.0%
GTTTCAATGTAGCTCTTAAT+AGG + chr8.3:3748953-3748972 MS.gene96314:CDS 30.0%
TAAGACTAAGTTCCTTCAAA+AGG + chr8.3:3748857-3748876 MS.gene96314:CDS 30.0%
TCTTAAGGTAAGTAAAGTGA+TGG - chr8.3:3748842-3748861 None:intergenic 30.0%
TTTCAGACAGAACAATTTCA+TGG + chr8.3:3749350-3749369 MS.gene96314:CDS 30.0%
!! ACACATGTTAAAAGCATTGT+TGG + chr8.3:3749807-3749826 MS.gene96314:intron 30.0%
!! ATGTTAAAAGCATTGTTGGT+TGG + chr8.3:3749811-3749830 MS.gene96314:intron 30.0%
!! TTTGAAGGAACTTAGTCTTA+AGG - chr8.3:3748857-3748876 None:intergenic 30.0%
AAAGCCCTTTAGCAGTAATA+AGG + chr8.3:3750170-3750189 MS.gene96314:CDS 35.0%
AGAAGAAAACATTGCCTTTC+TGG + chr8.3:3750274-3750293 MS.gene96314:CDS 35.0%
AGATGCTCATCTTGTAATCA+AGG - chr8.3:3750122-3750141 None:intergenic 35.0%
ATGCACCTTATTACTGCTAA+AGG - chr8.3:3750178-3750197 None:intergenic 35.0%
CTAAAAGGTTAGTGCATGTT+TGG - chr8.3:3749586-3749605 None:intergenic 35.0%
TGATGATCCTAGTGTTAGTA+GGG + chr8.3:3749155-3749174 MS.gene96314:CDS 35.0%
TGCACCTTATTACTGCTAAA+GGG - chr8.3:3750177-3750196 None:intergenic 35.0%
TGGCACAGAAGAACAAAATA+AGG + chr8.3:3750308-3750327 MS.gene96314:CDS 35.0%
TTGACTGATTAACGTGTAAG+AGG - chr8.3:3749906-3749925 None:intergenic 35.0%
TTGTTTCCAGCATATGAGAT+AGG - chr8.3:3750152-3750171 None:intergenic 35.0%
! AATGTGTTGAGGAGTATGTA+CGG + chr8.3:3749766-3749785 MS.gene96314:intron 35.0%
! TTGATGATCCTAGTGTTAGT+AGG + chr8.3:3749154-3749173 MS.gene96314:CDS 35.0%
! TTTGAAGCATTCTCTTGTGT+TGG + chr8.3:3748774-3748793 MS.gene96314:CDS 35.0%
!! AAAGTGTTGTCTAGTTCTGA+TGG + chr8.3:3749713-3749732 MS.gene96314:intron 35.0%
!! ATTTGTTGTGCAATGTGTTG+AGG + chr8.3:3749755-3749774 MS.gene96314:intron 35.0%
!! TGTTATAATAGTTCTGGTGG+TGG + chr8.3:3749261-3749280 MS.gene96314:CDS 35.0%
AAAGTGATGGCTACATGTAG+AGG - chr8.3:3748829-3748848 None:intergenic 40.0%
CTCATAACCCTACTAACACT+AGG - chr8.3:3749165-3749184 None:intergenic 40.0%
CTGTGATTCTCACTAGTTGA+AGG - chr8.3:3749309-3749328 None:intergenic 40.0%
GTGGATGTTGTGAAGTTTGA+TGG - chr8.3:3748909-3748928 None:intergenic 40.0%
TAGCAGTAATAAGGTGCATG+AGG + chr8.3:3750179-3750198 MS.gene96314:CDS 40.0%
TATGTACGGTTACTGAGCTA+TGG + chr8.3:3749780-3749799 MS.gene96314:intron 40.0%
TCACTAGTTGAAGGTGAACA+TGG - chr8.3:3749300-3749319 None:intergenic 40.0%
TCAGAACCTATCTCATATGC+TGG + chr8.3:3750143-3750162 MS.gene96314:CDS 40.0%
TGAGAGTGAATCAAAGGAGA+AGG - chr8.3:3748991-3749010 None:intergenic 40.0%
! AATGTTTTCTTCTGTCCTGC+AGG - chr8.3:3750267-3750286 None:intergenic 40.0%
! CTCGTCAACTCAGTTCTTTT+TGG - chr8.3:3749432-3749451 None:intergenic 40.0%
! CTTAAGACAAGCCCTTTTGA+AGG - chr8.3:3748872-3748891 None:intergenic 40.0%
! GTCAACTCAGTTCTTTTTGG+TGG - chr8.3:3749429-3749448 None:intergenic 40.0%
! TGTTCTGTCTGAAACTGCTA+AGG - chr8.3:3749344-3749363 None:intergenic 40.0%
!! AGCATTGTTGGTTGGTAGTA+AGG + chr8.3:3749819-3749838 MS.gene96314:intron 40.0%
!! ATCAAAGGAGAAGGTGTTGT+TGG - chr8.3:3748982-3749001 None:intergenic 40.0%
!! ATGAGAGCATTGGAAAGTGA+AGG - chr8.3:3749015-3749034 None:intergenic 40.0%
!! ATTCTCTTGTGTTGGCTAGA+AGG + chr8.3:3748782-3748801 MS.gene96314:CDS 40.0%
!! TCAAGAGCTCTAGATTGAAG+TGG - chr8.3:3748928-3748947 None:intergenic 40.0%
!! TGTTGTGAAGTTTGATGGTG+TGG - chr8.3:3748904-3748923 None:intergenic 40.0%
AGTAGGGTTATGAGAGAAGC+TGG + chr8.3:3749171-3749190 MS.gene96314:CDS 45.0%
GAAGGCTGAGAGTGAATCAA+AGG - chr8.3:3748997-3749016 None:intergenic 45.0%
GCACAAGCTCATCAAAGAAG+AGG + chr8.3:3749051-3749070 MS.gene96314:CDS 45.0%
! ACTGAGTTGACGAGTACATC+AGG + chr8.3:3749438-3749457 MS.gene96314:CDS 45.0%
! CAATGCAGCAATGAGAGCAT+TGG - chr8.3:3749025-3749044 None:intergenic 45.0%
ACATGTAGAGGAGTGACCTG+TGG - chr8.3:3748817-3748836 None:intergenic 50.0%
CCATGAGACTGAAGCCAGAA+AGG - chr8.3:3750291-3750310 None:intergenic 50.0%
GAGTTGACGAGTACATCAGG+TGG + chr8.3:3749441-3749460 MS.gene96314:CDS 50.0%
GCTCAATTTCTCAGACCTGC+AGG + chr8.3:3750249-3750268 MS.gene96314:CDS 50.0%
GCTTGACCCTTCATTCTTCC+AGG + chr8.3:3749469-3749488 MS.gene96314:CDS 50.0%
TAGAAGGAGAGGTCATCCAC+AGG + chr8.3:3748798-3748817 MS.gene96314:CDS 50.0%
TTGCTGCTGCTGCTGTTCTA+TGG - chr8.3:3749079-3749098 None:intergenic 50.0%
! ACGAGTACATCAGGTGGACT+TGG + chr8.3:3749447-3749466 MS.gene96314:CDS 50.0%
!! CCTTTCTGGCTTCAGTCTCA+TGG + chr8.3:3750288-3750307 MS.gene96314:CDS 50.0%
!! CTTGTGTTGGCTAGAAGGAG+AGG + chr8.3:3748787-3748806 MS.gene96314:CDS 50.0%
GCAGCAGCAGCAACAGCAAA+CGG + chr8.3:3749086-3749105 MS.gene96314:CDS 55.0%
TGAAGCAGCCTCTGCTGTGA+GGG - chr8.3:3748752-3748771 None:intergenic 55.0%
CTGAAGCAGCCTCTGCTGTG+AGG - chr8.3:3748753-3748772 None:intergenic 60.0%
GCAGCAGACCCTCACAGCAG+AGG + chr8.3:3748741-3748760 MS.gene96314:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 3748718 3750344 3748718 ID=MS.gene96314
chr8.3 mRNA 3748718 3750344 3748718 ID=MS.gene96314.t1;Parent=MS.gene96314
chr8.3 exon 3748718 3749490 3748718 ID=MS.gene96314.t1.exon1;Parent=MS.gene96314.t1
chr8.3 CDS 3748718 3749490 3748718 ID=cds.MS.gene96314.t1;Parent=MS.gene96314.t1
chr8.3 exon 3750116 3750344 3750116 ID=MS.gene96314.t1.exon2;Parent=MS.gene96314.t1
chr8.3 CDS 3750116 3750344 3750116 ID=cds.MS.gene96314.t1;Parent=MS.gene96314.t1
Gene Sequence

>MS.gene96314

ATGCGCTCAGGAGGTTGTGCATTGCAGCAGACCCTCACAGCAGAGGCTGCTTCAGTTTTGAAGCATTCTCTTGTGTTGGCTAGAAGGAGAGGTCATCCACAGGTCACTCCTCTACATGTAGCCATCACTTTACTTACCTTAAGACTAAGTTCCTTCAAAAGGGCTTGTCTTAAGTCTCATCAACCACACCATCAAACTTCACAACATCCACTTCAATCTAGAGCTCTTGAGCTTTGTTTCAATGTAGCTCTTAATAGGTTACCAACAACACCTTCTCCTTTGATTCACTCTCAGCCTTCACTTTCCAATGCTCTCATTGCTGCATTGAAAAGAGCACAAGCTCATCAAAGAAGAGGTTCCATAGAACAGCAGCAGCAGCAACAGCAAACGGTTCTTACTGTGAAAGTTGAACTTGAACAGCTTATTATATCTATACTTGATGATCCTAGTGTTAGTAGGGTTATGAGAGAAGCTGGATTCTCTAGTACTCTTGTTAAGAACAATTTAGAAGACTCTTTCTCACCTTCTAATTCTGTTTTTAAATGTTATAATAGTTCTGGTGGTGGTGTGTTTTCATCTCCATGTTCACCTTCAACTAGTGAGAATCACAGAGAAAACATCAACCTTAGCAGTTTCAGACAGAACAATTTCATGGCTGCTACTAGTGCTAAGCACAGTTCTTGTGAGTTTAACTCAGTTCTCTTTTCTCCACCAAAAAGAACTGAGTTGACGAGTACATCAGGTGGACTTGGCTTGACCCTTCATTCTTCCAGGTAATATTTTATTTTGCTTAATCAATAAATATAACACTTAGTACATGTTTGGATTCACAGTGAGTTAGACAGAAACACAGTGATTTGTGTATCCAAACATGCACTAACCTTTTAGTAACATAACATGTGTTCTTAATTTGTTTGATTTAAGGTATAAAAGGTTTCATAATGACATATTAAATGCAACCAAAAAAGAAAGGTAAACATAAGTACATAAAACTAAAAGTGTTGTCTAGTTCTGATGGTGTTTTTGAAATGTTATTAATTTGTTGTGCAATGTGTTGAGGAGTATGTACGGTTACTGAGCTATGGCAGAACACATGTTAAAAGCATTGTTGGTTGGTAGTAAGGCAAATATATGAAGAAGAGTCTTTTTTTTCTGCATCACTTTAGTTTGTCAGTATATAACAAACCTCTTACACGTTAATCAGTCAAATTATTTTGAGTTGGAGAAAAACAAAGACTACACTTTATCTAAAGGAAAATAAAGAAGCAAAGAGTAATACTATTTTCTTTTTCATATGACAAAAAAGCATTTTCTGAGTAGTATGTATGTTTAAAACTCAACAGTGCCTATTTATTTGCTTAATTATGGATGTTAATTTCTTTTGTACTTAATTTGCAGTGTCCTTGATTACAAGATGAGCATCTCTCAGAACCTATCTCATATGCTGGAAACAAAGCCCTTTAGCAGTAATAAGGTGCATGAGGATAAACTTAATTGTTGTGAAGAATGTGCTTCCAATTATGAAAAAGAAGCTCAATTTCTCAGACCTGCAGGACAGAAGAAAACATTGCCTTTCTGGCTTCAGTCTCATGGCACAGAAGAACAAAATAAGGTAGCAAATCATTGA

Protein sequence

>MS.gene96314.t1

MRSGGCALQQTLTAEAASVLKHSLVLARRRGHPQVTPLHVAITLLTLRLSSFKRACLKSHQPHHQTSQHPLQSRALELCFNVALNRLPTTPSPLIHSQPSLSNALIAALKRAQAHQRRGSIEQQQQQQQTVLTVKVELEQLIISILDDPSVSRVMREAGFSSTLVKNNLEDSFSPSNSVFKCYNSSGGGVFSSPCSPSTSENHRENINLSSFRQNNFMAATSAKHSSCEFNSVLFSPPKRTELTSTSGGLGLTLHSSSVLDYKMSISQNLSHMLETKPFSSNKVHEDKLNCCEECASNYEKEAQFLRPAGQKKTLPFWLQSHGTEEQNKVANH