Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96512.t1 | AGC78837.1 | 93.8 | 259 | 16 | 0 | 26 | 284 | 1 | 259 | 5.50E-133 | 483.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96512.t1 | P05491 | 96.0 | 252 | 6 | 2 | 34 | 284 | 12 | 260 | 7.2e-134 | 478.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96512.t1 | R4IUC9 | 93.8 | 259 | 16 | 0 | 26 | 284 | 1 | 259 | 3.9e-133 | 483.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050945 | MS.gene96512 | -0.810874 | 9.28E-51 | -1.69E-46 |
| MS.gene053931 | MS.gene96512 | -0.806601 | 7.60E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96512.t1 | MTR_0212s0040 | 94.779 | 249 | 1 | 1 | 36 | 284 | 620 | 856 | 1.15e-166 | 484 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96512.t1 | ATMG00160 | 88.764 | 267 | 23 | 2 | 18 | 284 | 1 | 260 | 8.16e-172 | 475 |
| MS.gene96512.t1 | AT2G07695 | 73.148 | 108 | 22 | 2 | 18 | 125 | 1 | 101 | 1.36e-43 | 150 |
Find 0 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAAATCAGATCTCTATTT+CGG | + | 6898:10378-10397 | MS.gene96512:CDS | 20.0% |
| ! | TAAGCAAAGGATAATTAGTA+GGG | - | 6898:9744-9763 | None:intergenic | 25.0% |
| !! | AATAATACGTAGATGAGTTT+TGG | - | 6898:10284-10303 | None:intergenic | 25.0% |
| !! | ATTTGCTCTTTTATACTCAA+TGG | + | 6898:10066-10085 | MS.gene96512:CDS | 25.0% |
| AATCAATTAATCCCACAAAC+AGG | + | 6898:10517-10536 | MS.gene96512:CDS | 30.0% | |
| ATCAATTAATCCCACAAACA+GGG | + | 6898:10518-10537 | MS.gene96512:CDS | 30.0% | |
| CTAAGCAAAGGATAATTAGT+AGG | - | 6898:9745-9764 | None:intergenic | 30.0% | |
| CTAGTAAAGATTATGGTTCT+CGG | + | 6898:10488-10507 | MS.gene96512:CDS | 30.0% | |
| CTTGAAATCCTAATTGCAAT+GGG | - | 6898:9823-9842 | None:intergenic | 30.0% | |
| GAGTATAAAAGAGCAAATGA+TGG | - | 6898:10064-10083 | None:intergenic | 30.0% | |
| TAGTAAAGATTATGGTTCTC+GGG | + | 6898:10489-10508 | MS.gene96512:CDS | 30.0% | |
| TCAATCACGTTTATTAGAAG+TGG | + | 6898:10237-10256 | MS.gene96512:CDS | 30.0% | |
| TCTATTATTCCTTGCATCAT+AGG | - | 6898:9857-9876 | None:intergenic | 30.0% | |
| TCTTGAAATCCTAATTGCAA+TGG | - | 6898:9824-9843 | None:intergenic | 30.0% | |
| TGTTTGTGGGATTAATTGAT+TGG | - | 6898:10518-10537 | None:intergenic | 30.0% | |
| ! | TTTCTTCCTGATTCTGATTT+TGG | + | 6898:9895-9914 | MS.gene96512:CDS | 30.0% |
| ! | TTTTGACAGTTATACGATTC+CGG | + | 6898:10195-10214 | MS.gene96512:CDS | 30.0% |
| !!! | TGATTTTGGTTTTCGTATCA+CGG | + | 6898:9909-9928 | MS.gene96512:CDS | 30.0% |
| AAAAGTGAGTGACTGTTCAT+CGG | - | 6898:10179-10198 | None:intergenic | 35.0% | |
| ATGGTATAGCAATGAACATC+GGG | - | 6898:10045-10064 | None:intergenic | 35.0% | |
| ATTAGAAGTGGACAATAGAG+TGG | + | 6898:10249-10268 | MS.gene96512:CDS | 35.0% | |
| CGAAAACCAAAATCAGAATC+AGG | - | 6898:9904-9923 | None:intergenic | 35.0% | |
| CGAGAACCATAATCTTTACT+AGG | - | 6898:10490-10509 | None:intergenic | 35.0% | |
| CTATTGGACATCAATGGTAT+CGG | + | 6898:10125-10144 | MS.gene96512:CDS | 35.0% | |
| CTATTTCGGTACAAAGAGAA+GGG | + | 6898:10392-10411 | MS.gene96512:CDS | 35.0% | |
| GAACTACTATCGAGATTCTT+CGG | + | 6898:9999-10018 | MS.gene96512:CDS | 35.0% | |
| GATGGTATAGCAATGAACAT+CGG | - | 6898:10046-10065 | None:intergenic | 35.0% | |
| GCTGTTCCTAGTAAAGATTA+TGG | + | 6898:10481-10500 | MS.gene96512:CDS | 35.0% | |
| GGGAATTGAAGAAGATCAAT+CGG | - | 6898:9776-9795 | None:intergenic | 35.0% | |
| GTTCCATGAACAATTCTTTG+CGG | - | 6898:9983-10002 | None:intergenic | 35.0% | |
| TATTTCGGTACAAAGAGAAG+GGG | + | 6898:10393-10412 | MS.gene96512:CDS | 35.0% | |
| TCAAAGCTATTGGACATCAA+TGG | + | 6898:10119-10138 | MS.gene96512:CDS | 35.0% | |
| TCAATTAATCCCACAAACAG+GGG | + | 6898:10519-10538 | MS.gene96512:CDS | 35.0% | |
| TCTATTTCGGTACAAAGAGA+AGG | + | 6898:10391-10410 | MS.gene96512:CDS | 35.0% | |
| TTCCATGAACAATTCTTTGC+GGG | - | 6898:9982-10001 | None:intergenic | 35.0% | |
| ! | TCTTTTATACTCAATGGACG+AGG | + | 6898:10072-10091 | MS.gene96512:CDS | 35.0% |
| !! | TCCAATAGCTTTGATAGTCA+TGG | - | 6898:10113-10132 | None:intergenic | 35.0% |
| AGCATCACATTTGACACCTA+AGG | - | 6898:10347-10366 | None:intergenic | 40.0% | |
| ATCCCGCAAAGAATTGTTCA+TGG | + | 6898:9977-9996 | MS.gene96512:CDS | 40.0% | |
| ATGAACATCGGGATGATACT+AGG | - | 6898:10034-10053 | None:intergenic | 40.0% | |
| CAAAGAGAAGGGGTTTACTA+TGG | + | 6898:10403-10422 | MS.gene96512:CDS | 40.0% | |
| CATCCTGCGAAGAAAATCTT+GGG | - | 6898:9711-9730 | None:intergenic | 40.0% | |
| CCCAATTCTAGATCATCTTC+CGG | - | 6898:10217-10236 | None:intergenic | 40.0% | |
| CGGGATGATACTAGGAAATA+TGG | - | 6898:10026-10045 | None:intergenic | 40.0% | |
| GAACAATTCTTTGCGGGATT+GGG | - | 6898:9976-9995 | None:intergenic | 40.0% | |
| GAAGAAGATCAATCGGAAGA+AGG | - | 6898:9769-9788 | None:intergenic | 40.0% | |
| GAGATCTGATTTAAACGACC+AGG | - | 6898:10373-10392 | None:intergenic | 40.0% | |
| GCCATGACTATCAAAGCTAT+TGG | + | 6898:10109-10128 | MS.gene96512:CDS | 40.0% | |
| TCACATTTGACACCTAAGGA+AGG | - | 6898:10343-10362 | None:intergenic | 40.0% | |
| TGAACAATTCTTTGCGGGAT+TGG | - | 6898:9977-9996 | None:intergenic | 40.0% | |
| TGCTGATGTACCTCATAGTT+GGG | + | 6898:10312-10331 | MS.gene96512:CDS | 40.0% | |
| ! | ATACCCAAGATTTTCTTCGC+AGG | + | 6898:9705-9724 | MS.gene96512:CDS | 40.0% |
| ! | CCGGAAGATGATCTAGAATT+GGG | + | 6898:10214-10233 | MS.gene96512:CDS | 40.0% |
| ! | TCCGGAAGATGATCTAGAAT+TGG | + | 6898:10213-10232 | MS.gene96512:CDS | 40.0% |
| !! | ATACGTAGATGAGTTTTGGC+TGG | - | 6898:10280-10299 | None:intergenic | 40.0% |
| !! | ATAGCTTTGATAGTCATGGC+TGG | - | 6898:10109-10128 | None:intergenic | 40.0% |
| CTGCTGATGTACCTCATAGT+TGG | + | 6898:10311-10330 | MS.gene96512:CDS | 45.0% | |
| GCATCCTGCGAAGAAAATCT+TGG | - | 6898:9712-9731 | None:intergenic | 45.0% | |
| GTCAAATGTGATGCTGTACC+TGG | + | 6898:10352-10371 | MS.gene96512:CDS | 45.0% | |
| TCGGAAGAAGGACTAAGCAA+AGG | - | 6898:9757-9776 | None:intergenic | 45.0% | |
| TTCTACGACGATAGGCGTAA+AGG | - | 6898:10461-10480 | None:intergenic | 45.0% | |
| ! | GGTTTTCGTATCACGGATCT+TGG | + | 6898:9916-9935 | MS.gene96512:CDS | 45.0% |
| AGTTGGGCTGTACCTTCCTT+AGG | + | 6898:10328-10347 | MS.gene96512:CDS | 50.0% | |
| GCAGCAACACCTATGATGCA+AGG | + | 6898:9845-9864 | MS.gene96512:CDS | 50.0% | |
| GGAACAGCTTCTACGACGAT+AGG | - | 6898:10469-10488 | None:intergenic | 50.0% | |
| GGTCAGTGCAGTGAGATTTG+TGG | + | 6898:10424-10443 | MS.gene96512:CDS | 50.0% | |
| ! | GAAGGTACAGCCCAACTATG+AGG | - | 6898:10325-10344 | None:intergenic | 50.0% |
| CTGCGCTCTTTCTGATGCAG+CGG | + | 6898:9796-9815 | MS.gene96512:CDS | 55.0% | |
| GCAGCGGACCCATTGCAATT+AGG | + | 6898:9812-9831 | MS.gene96512:CDS | 55.0% | |
| ! | CTGCATCAGAAAGAGCGCAG+GGG | - | 6898:9796-9815 | None:intergenic | 55.0% |
| ! | GCTGCATCAGAAAGAGCGCA+GGG | - | 6898:9797-9816 | None:intergenic | 55.0% |
| ! | GGATCTTGGTTCGCGCTTTG+TGG | + | 6898:9930-9949 | MS.gene96512:CDS | 55.0% |
| ! | CGCTGCATCAGAAAGAGCGC+AGG | - | 6898:9798-9817 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 6898 | gene | 9695 | 10549 | 9695 | ID=MS.gene96512 |
| 6898 | mRNA | 9695 | 10549 | 9695 | ID=MS.gene96512.t1;Parent=MS.gene96512 |
| 6898 | exon | 9695 | 10549 | 9695 | ID=MS.gene96512.t1.exon1;Parent=MS.gene96512.t1 |
| 6898 | CDS | 9695 | 10549 | 9695 | ID=cds.MS.gene96512.t1;Parent=MS.gene96512.t1 |
| Gene Sequence |
| Protein sequence |
