Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96527.t1 | XP_003614502.1 | 98.3 | 121 | 1 | 1 | 1 | 121 | 37 | 156 | 2.20E-59 | 238 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96527.t1 | Q9SX29 | 78.5 | 121 | 26 | 0 | 1 | 121 | 39 | 159 | 9.5e-51 | 200.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96527.t1 | G7JYE1 | 98.3 | 121 | 1 | 1 | 1 | 121 | 37 | 156 | 1.6e-59 | 238.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053895 | MS.gene96527 | 0.943182 | 1.91E-102 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96527.t1 | MTR_5g054770 | 98.347 | 121 | 1 | 1 | 1 | 121 | 37 | 156 | 2.74e-84 | 243 |
| MS.gene96527.t1 | MTR_6g085050 | 80.000 | 115 | 23 | 0 | 7 | 121 | 46 | 160 | 1.70e-67 | 201 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96527.t1 | AT1G68660 | 78.512 | 121 | 26 | 0 | 1 | 121 | 39 | 159 | 4.94e-70 | 207 |
| MS.gene96527.t1 | AT1G68660 | 57.851 | 121 | 21 | 1 | 1 | 121 | 39 | 129 | 4.07e-43 | 138 |
Find 37 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGGTGGCTTTCTCAATAAC+TGG | 0.289306 | 5.1:-42923658 | None:intergenic |
| TCAAATCAAATTCAGATTCC+CGG | 0.395114 | 5.1:-42923684 | None:intergenic |
| GATCATTGTATGCAACTTAG+AGG | 0.399829 | 5.1:+42924428 | MS.gene96527:CDS |
| TTGTGCAATATTACACGATA+TGG | 0.414798 | 5.1:-42924257 | None:intergenic |
| TAACAAAAGGATCTGGTAAA+GGG | 0.423712 | 5.1:+42923614 | MS.gene96527:CDS |
| GCATTATCAAGGGTCATTCC+AGG | 0.425052 | 5.1:-42924332 | None:intergenic |
| TCAAATTCAGATTCCCGGCC+TGG | 0.431777 | 5.1:-42923679 | None:intergenic |
| TGATGTTAACTGCATTATCA+AGG | 0.464225 | 5.1:-42924343 | None:intergenic |
| CCCATGGCTGTATTAACAAA+AGG | 0.470418 | 5.1:+42923601 | None:intergenic |
| CAAATTCAGATTCCCGGCCT+GGG | 0.480296 | 5.1:-42923678 | None:intergenic |
| GCTGTATTAACAAAAGGATC+TGG | 0.483670 | 5.1:+42923607 | MS.gene96527:CDS |
| TTAACAAAAGGATCTGGTAA+AGG | 0.488662 | 5.1:+42923613 | MS.gene96527:CDS |
| TGAGAAAGCCACCCCAGGCC+GGG | 0.496954 | 5.1:+42923666 | MS.gene96527:CDS |
| TGTATGCAACTTAGAGGCAA+TGG | 0.502603 | 5.1:+42924434 | MS.gene96527:CDS |
| CTCAGTTCAATTGAACCTGC+AGG | 0.507343 | 5.1:+42924461 | MS.gene96527:CDS |
| AATGATCTTCAGCATCAGCT+TGG | 0.508043 | 5.1:-42924412 | None:intergenic |
| ATGATCTTCAGCATCAGCTT+GGG | 0.510156 | 5.1:-42924411 | None:intergenic |
| TTCAGATTCCCGGCCTGGGG+TGG | 0.510475 | 5.1:-42923674 | None:intergenic |
| AACAAAAGGATCTGGTAAAG+GGG | 0.514249 | 5.1:+42923615 | MS.gene96527:CDS |
| TTGAGAAAGCCACCCCAGGC+CGG | 0.534808 | 5.1:+42923665 | MS.gene96527:CDS |
| GGATCACCTTCATAAGTACT+TGG | 0.538715 | 5.1:-42924310 | None:intergenic |
| CTAAGATGTGCAGGAAATCA+CGG | 0.554342 | 5.1:+42924222 | MS.gene96527:intron |
| CATCAGCAACCATCACCTGC+AGG | 0.555211 | 5.1:-42924476 | None:intergenic |
| TGTTGTCCAAGTACTTATGA+AGG | 0.564357 | 5.1:+42924304 | MS.gene96527:CDS |
| GTACTTATGAAGGTGATCCC+TGG | 0.569299 | 5.1:+42924314 | MS.gene96527:CDS |
| GTTATTGAGAAAGCCACCCC+AGG | 0.591201 | 5.1:+42923661 | MS.gene96527:CDS |
| TCAATTGAACCTGCAGGTGA+TGG | 0.594938 | 5.1:+42924467 | MS.gene96527:CDS |
| CATTATCAAGGGTCATTCCA+GGG | 0.602128 | 5.1:-42924331 | None:intergenic |
| GATGTTAACTGCATTATCAA+GGG | 0.610327 | 5.1:-42924342 | None:intergenic |
| CAATGACAACTTCAACAGAC+GGG | 0.612734 | 5.1:+42924277 | MS.gene96527:CDS |
| TGCAATATTACACGATATGG+CGG | 0.626220 | 5.1:-42924254 | None:intergenic |
| ACAATGACAACTTCAACAGA+CGG | 0.629056 | 5.1:+42924276 | MS.gene96527:CDS |
| ATGCAAGAAGCTCACCACAA+TGG | 0.636426 | 5.1:+42924365 | MS.gene96527:CDS |
| AAATTCAGATTCCCGGCCTG+GGG | 0.643776 | 5.1:-42923677 | None:intergenic |
| ACAAAAGGATCTGGTAAAGG+GGG | 0.662590 | 5.1:+42923616 | MS.gene96527:CDS |
| TCACCACAATGGAATGTCAG+TGG | 0.665125 | 5.1:+42924376 | MS.gene96527:CDS |
| TCACCACTGACATTCCATTG+TGG | 0.707546 | 5.1:-42924379 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTTATGATGATTTTCAA+TGG | + | chr5.1:42923869-42923888 | MS.gene96527:intron | 15.0% |
| !!! | AATTTATGATGATTTTCAAT+GGG | + | chr5.1:42923870-42923889 | MS.gene96527:intron | 15.0% |
| !! | AGTCATTTAGAAAATATGAT+AGG | + | chr5.1:42924043-42924062 | MS.gene96527:intron | 20.0% |
| !! | TTATTAGTGATGATAACTTA+AGG | + | chr5.1:42923899-42923918 | MS.gene96527:intron | 20.0% |
| !!! | AAAAAGAAAGTATTTGATTC+TGG | + | chr5.1:42924194-42924213 | MS.gene96527:intron | 20.0% |
| ! | AAGTTATCATCACTAATAAC+TGG | - | chr5.1:42923898-42923917 | None:intergenic | 25.0% |
| !! | ACAAGTAATACTTGATGTTT+TGG | + | chr5.1:42923955-42923974 | MS.gene96527:intron | 25.0% |
| !!! | CATCAAGTATTACTTGTTTT+AGG | - | chr5.1:42923952-42923971 | None:intergenic | 25.0% |
| !!! | TTGTTTTTCTGAATGTGATT+AGG | + | chr5.1:42923768-42923787 | MS.gene96527:intron | 25.0% |
| CAAGTATCTTCAAATGTCAA+TGG | - | chr5.1:42923834-42923853 | None:intergenic | 30.0% | |
| CTTAACGATGAAATTGAAGT+TGG | + | chr5.1:42924082-42924101 | MS.gene96527:intron | 30.0% | |
| GATGTTAACTGCATTATCAA+GGG | - | chr5.1:42924345-42924364 | None:intergenic | 30.0% | |
| TAACAAAAGGATCTGGTAAA+GGG | + | chr5.1:42923614-42923633 | MS.gene96527:CDS | 30.0% | |
| TCAAATCAAATTCAGATTCC+CGG | - | chr5.1:42923687-42923706 | None:intergenic | 30.0% | |
| TGATGTTAACTGCATTATCA+AGG | - | chr5.1:42924346-42924365 | None:intergenic | 30.0% | |
| TTAACAAAAGGATCTGGTAA+AGG | + | chr5.1:42923613-42923632 | MS.gene96527:CDS | 30.0% | |
| TTGTGCAATATTACACGATA+TGG | - | chr5.1:42924260-42924279 | None:intergenic | 30.0% | |
| !! | AGATACTTGTTATTGTGCAA+TGG | + | chr5.1:42923845-42923864 | MS.gene96527:intron | 30.0% |
| !! | GATACTTGTTATTGTGCAAT+GGG | + | chr5.1:42923846-42923865 | MS.gene96527:intron | 30.0% |
| AACAAAAGGATCTGGTAAAG+GGG | + | chr5.1:42923615-42923634 | MS.gene96527:CDS | 35.0% | |
| ACAATGACAACTTCAACAGA+CGG | + | chr5.1:42924276-42924295 | MS.gene96527:CDS | 35.0% | |
| ATGTCATCGCATGTAGTAAA+AGG | + | chr5.1:42923923-42923942 | MS.gene96527:intron | 35.0% | |
| GATCATTGTATGCAACTTAG+AGG | + | chr5.1:42924428-42924447 | MS.gene96527:CDS | 35.0% | |
| GCTGTATTAACAAAAGGATC+TGG | + | chr5.1:42923607-42923626 | MS.gene96527:CDS | 35.0% | |
| TATGATAGGATGTTTAAGCG+TGG | + | chr5.1:42924057-42924076 | MS.gene96527:intron | 35.0% | |
| TGCAATATTACACGATATGG+CGG | - | chr5.1:42924257-42924276 | None:intergenic | 35.0% | |
| TGTTGTCCAAGTACTTATGA+AGG | + | chr5.1:42924304-42924323 | MS.gene96527:CDS | 35.0% | |
| AATGATCTTCAGCATCAGCT+TGG | - | chr5.1:42924415-42924434 | None:intergenic | 40.0% | |
| ACAAAAGGATCTGGTAAAGG+GGG | + | chr5.1:42923616-42923635 | MS.gene96527:CDS | 40.0% | |
| ATGATCTTCAGCATCAGCTT+GGG | - | chr5.1:42924414-42924433 | None:intergenic | 40.0% | |
| CAATGACAACTTCAACAGAC+GGG | + | chr5.1:42924277-42924296 | MS.gene96527:CDS | 40.0% | |
| CATTATCAAGGGTCATTCCA+GGG | - | chr5.1:42924334-42924353 | None:intergenic | 40.0% | |
| CGACAACTAAATCACTACAG+TGG | - | chr5.1:42924112-42924131 | None:intergenic | 40.0% | |
| CTAAGATGTGCAGGAAATCA+CGG | + | chr5.1:42924222-42924241 | MS.gene96527:intron | 40.0% | |
| GGATCACCTTCATAAGTACT+TGG | - | chr5.1:42924313-42924332 | None:intergenic | 40.0% | |
| TGTATGCAACTTAGAGGCAA+TGG | + | chr5.1:42924434-42924453 | MS.gene96527:CDS | 40.0% | |
| ! | TGTTGTTCTGTTGTGCTGAT+TGG | + | chr5.1:42924132-42924151 | MS.gene96527:intron | 40.0% |
| !!! | TCTCAATAACTGGCTTTTCC+AGG | - | chr5.1:42923651-42923670 | None:intergenic | 40.0% |
| ATGCAAGAAGCTCACCACAA+TGG | + | chr5.1:42924365-42924384 | MS.gene96527:CDS | 45.0% | |
| CTCAGTTCAATTGAACCTGC+AGG | + | chr5.1:42924461-42924480 | MS.gene96527:CDS | 45.0% | |
| GCATTATCAAGGGTCATTCC+AGG | - | chr5.1:42924335-42924354 | None:intergenic | 45.0% | |
| GTACTTATGAAGGTGATCCC+TGG | + | chr5.1:42924314-42924333 | MS.gene96527:CDS | 45.0% | |
| TCAATTGAACCTGCAGGTGA+TGG | + | chr5.1:42924467-42924486 | MS.gene96527:CDS | 45.0% | |
| TCACCACAATGGAATGTCAG+TGG | + | chr5.1:42924376-42924395 | MS.gene96527:CDS | 45.0% | |
| TCACCACTGACATTCCATTG+TGG | - | chr5.1:42924382-42924401 | None:intergenic | 45.0% | |
| !! | CTGGCATTTCTAAGATGTGC+AGG | + | chr5.1:42924213-42924232 | MS.gene96527:intron | 45.0% |
| AAAGGATCTGGTAAAGGGGG+AGG | + | chr5.1:42923619-42923638 | MS.gene96527:CDS | 50.0% | |
| AAATTCAGATTCCCGGCCTG+GGG | - | chr5.1:42923680-42923699 | None:intergenic | 50.0% | |
| CAAATTCAGATTCCCGGCCT+GGG | - | chr5.1:42923681-42923700 | None:intergenic | 50.0% | |
| GGGGTGGCTTTCTCAATAAC+TGG | - | chr5.1:42923661-42923680 | None:intergenic | 50.0% | |
| GTTATTGAGAAAGCCACCCC+AGG | + | chr5.1:42923661-42923680 | MS.gene96527:CDS | 50.0% | |
| TCAAATTCAGATTCCCGGCC+TGG | - | chr5.1:42923682-42923701 | None:intergenic | 50.0% | |
| TAAAGGGGGAGGAGGAATCC+TGG | + | chr5.1:42923630-42923649 | MS.gene96527:CDS | 55.0% | |
| GGATCTGGTAAAGGGGGAGG+AGG | + | chr5.1:42923622-42923641 | MS.gene96527:CDS | 60.0% | |
| TTGAGAAAGCCACCCCAGGC+CGG | + | chr5.1:42923665-42923684 | MS.gene96527:CDS | 60.0% | |
| TGAGAAAGCCACCCCAGGCC+GGG | + | chr5.1:42923666-42923685 | MS.gene96527:CDS | 65.0% | |
| TTCAGATTCCCGGCCTGGGG+TGG | - | chr5.1:42923677-42923696 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 42923604 | 42924496 | 42923604 | ID=MS.gene96527 |
| chr5.1 | mRNA | 42923604 | 42924496 | 42923604 | ID=MS.gene96527.t1;Parent=MS.gene96527 |
| chr5.1 | exon | 42923604 | 42923707 | 42923604 | ID=MS.gene96527.t1.exon1;Parent=MS.gene96527.t1 |
| chr5.1 | CDS | 42923604 | 42923707 | 42923604 | ID=cds.MS.gene96527.t1;Parent=MS.gene96527.t1 |
| chr5.1 | exon | 42924235 | 42924496 | 42924235 | ID=MS.gene96527.t1.exon2;Parent=MS.gene96527.t1 |
| chr5.1 | CDS | 42924235 | 42924496 | 42924235 | ID=cds.MS.gene96527.t1;Parent=MS.gene96527.t1 |
| Gene Sequence |
| Protein sequence |
