Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97236.t1 | XP_013467004.1 | 98.7 | 75 | 1 | 0 | 1 | 75 | 1 | 75 | 1.20E-33 | 152.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97236.t1 | A0A072VG82 | 98.7 | 75 | 1 | 0 | 1 | 75 | 1 | 75 | 8.3e-34 | 152.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057287 | MS.gene97236 | 0.818771 | 1.65E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97236.t1 | MTR_1g041155 | 98.851 | 87 | 1 | 0 | 1 | 87 | 1 | 87 | 6.23e-58 | 181 |
| MS.gene97236.t1 | MTR_1g076500 | 45.794 | 107 | 36 | 5 | 1 | 87 | 1 | 105 | 2.86e-15 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97236.t1 | AT3G22970 | 45.455 | 99 | 38 | 3 | 4 | 87 | 5 | 102 | 2.66e-14 | 66.6 |
Find 26 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTGTTTGCTTGGCGAAAA+TGG | 0.121680 | 1.3:-23972478 | MS.gene97236:CDS |
| TTCAACCTCCACTTCGAAAC+TGG | 0.330861 | 1.3:+23972585 | None:intergenic |
| TTCTCTGAATCTATGTCAAT+CGG | 0.419113 | 1.3:+23972636 | None:intergenic |
| TCTTTAGTGGTATTGTTCTT+CGG | 0.421271 | 1.3:+23972534 | None:intergenic |
| GTGGTATTGTTCTTCGGAAA+AGG | 0.429831 | 1.3:+23972540 | None:intergenic |
| TCCACCACCACCATCTTTAG+TGG | 0.439948 | 1.3:+23972521 | None:intergenic |
| TTCGCCAAGCAAACAGAGCT+TGG | 0.466708 | 1.3:+23972483 | None:intergenic |
| GGAACCAAGCTCTGTTTGCT+TGG | 0.476354 | 1.3:-23972487 | MS.gene97236:CDS |
| AGAGAAAGCCACTACGGAAC+TGG | 0.478102 | 1.3:-23972619 | MS.gene97236:CDS |
| CGAGACGGTGGTTGAAGTTG+CGG | 0.502860 | 1.3:+23972426 | None:intergenic |
| AAGAACAATACCACTAAAGA+TGG | 0.505083 | 1.3:-23972531 | MS.gene97236:CDS |
| TGGTGGTGGAGTTGCTGAGT+TGG | 0.512259 | 1.3:-23972508 | MS.gene97236:CDS |
| ACCACTAAAGATGGTGGTGG+TGG | 0.516726 | 1.3:-23972522 | MS.gene97236:CDS |
| GAAAATGGTTCAGAGTTTCA+TGG | 0.527012 | 1.3:-23972463 | MS.gene97236:CDS |
| GGTGGTTGAAGTTGCGGTTG+CGG | 0.536574 | 1.3:+23972432 | None:intergenic |
| CTGAATCTATGTCAATCGGT+TGG | 0.551079 | 1.3:+23972640 | None:intergenic |
| TTTCGAAGTGGAGGTTGAAG+AGG | 0.556890 | 1.3:-23972581 | MS.gene97236:CDS |
| ATGAACCAGTTTCGAAGTGG+AGG | 0.599985 | 1.3:-23972590 | MS.gene97236:CDS |
| ATTGCGAACCAGTTCCGTAG+TGG | 0.610392 | 1.3:+23972611 | None:intergenic |
| AGATTCAGAGAAAGCCACTA+CGG | 0.617727 | 1.3:-23972625 | MS.gene97236:CDS |
| GCAATGAACCAGTTTCGAAG+TGG | 0.634899 | 1.3:-23972593 | MS.gene97236:CDS |
| AACAATACCACTAAAGATGG+TGG | 0.638792 | 1.3:-23972528 | MS.gene97236:CDS |
| TCAATCGGTTGGATCTTCAA+CGG | 0.649259 | 1.3:+23972651 | None:intergenic |
| CAGTTACAGCGGTTACGAGA+CGG | 0.650994 | 1.3:+23972411 | None:intergenic |
| AATACCACTAAAGATGGTGG+TGG | 0.666485 | 1.3:-23972525 | MS.gene97236:CDS |
| TTACAGCGGTTACGAGACGG+TGG | 0.731805 | 1.3:+23972414 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGAACAATACCACTAAAGA+TGG | - | chr1.3:23972544-23972563 | MS.gene97236:CDS | 30.0% | |
| TCTTTAGTGGTATTGTTCTT+CGG | + | chr1.3:23972544-23972563 | None:intergenic | 30.0% | |
| TTCTCTGAATCTATGTCAAT+CGG | + | chr1.3:23972442-23972461 | None:intergenic | 30.0% | |
| AACAATACCACTAAAGATGG+TGG | - | chr1.3:23972547-23972566 | MS.gene97236:CDS | 35.0% | |
| GAAAATGGTTCAGAGTTTCA+TGG | - | chr1.3:23972612-23972631 | MS.gene97236:CDS | 35.0% | |
| AGATTCAGAGAAAGCCACTA+CGG | - | chr1.3:23972450-23972469 | MS.gene97236:CDS | 40.0% | |
| CTGAATCTATGTCAATCGGT+TGG | + | chr1.3:23972438-23972457 | None:intergenic | 40.0% | |
| GTGGTATTGTTCTTCGGAAA+AGG | + | chr1.3:23972538-23972557 | None:intergenic | 40.0% | |
| TCAATCGGTTGGATCTTCAA+CGG | + | chr1.3:23972427-23972446 | None:intergenic | 40.0% | |
| ! | AATACCACTAAAGATGGTGG+TGG | - | chr1.3:23972550-23972569 | MS.gene97236:CDS | 40.0% |
| ATGAACCAGTTTCGAAGTGG+AGG | - | chr1.3:23972485-23972504 | MS.gene97236:CDS | 45.0% | |
| CTCTGTTTGCTTGGCGAAAA+TGG | - | chr1.3:23972597-23972616 | MS.gene97236:CDS | 45.0% | |
| GCAATGAACCAGTTTCGAAG+TGG | - | chr1.3:23972482-23972501 | MS.gene97236:CDS | 45.0% | |
| TTCAACCTCCACTTCGAAAC+TGG | + | chr1.3:23972493-23972512 | None:intergenic | 45.0% | |
| TTTCGAAGTGGAGGTTGAAG+AGG | - | chr1.3:23972494-23972513 | MS.gene97236:CDS | 45.0% | |
| AGAGAAAGCCACTACGGAAC+TGG | - | chr1.3:23972456-23972475 | MS.gene97236:CDS | 50.0% | |
| ATTGCGAACCAGTTCCGTAG+TGG | + | chr1.3:23972467-23972486 | None:intergenic | 50.0% | |
| GGAACCAAGCTCTGTTTGCT+TGG | - | chr1.3:23972588-23972607 | MS.gene97236:CDS | 50.0% | |
| TCCACCACCACCATCTTTAG+TGG | + | chr1.3:23972557-23972576 | None:intergenic | 50.0% | |
| TTCGCCAAGCAAACAGAGCT+TGG | + | chr1.3:23972595-23972614 | None:intergenic | 50.0% | |
| !! | ACCACTAAAGATGGTGGTGG+TGG | - | chr1.3:23972553-23972572 | MS.gene97236:CDS | 50.0% |
| CGAGACGGTGGTTGAAGTTG+CGG | + | chr1.3:23972652-23972671 | None:intergenic | 55.0% | |
| GGTGGTTGAAGTTGCGGTTG+CGG | + | chr1.3:23972646-23972665 | None:intergenic | 55.0% | |
| !! | TGGTGGTGGAGTTGCTGAGT+TGG | - | chr1.3:23972567-23972586 | MS.gene97236:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 23972418 | 23972679 | 23972418 | ID=MS.gene97236 |
| chr1.3 | mRNA | 23972418 | 23972679 | 23972418 | ID=MS.gene97236.t1;Parent=MS.gene97236 |
| chr1.3 | exon | 23972418 | 23972679 | 23972418 | ID=MS.gene97236.t1.exon1;Parent=MS.gene97236.t1 |
| chr1.3 | CDS | 23972418 | 23972679 | 23972418 | ID=cds.MS.gene97236.t1;Parent=MS.gene97236.t1 |
| Gene Sequence |
| Protein sequence |
