Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057287.t1 | XP_013467004.1 | 80.8 | 360 | 11 | 5 | 1 | 305 | 1 | 357 | 3.70E-143 | 517.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057287.t1 | A0A072VG82 | 80.8 | 360 | 11 | 5 | 1 | 305 | 1 | 357 | 2.7e-143 | 517.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049808 | MS.gene057287 | 0.83334 | 5.64E-56 | -1.69E-46 |
| MS.gene050807 | MS.gene057287 | 0.800894 | 1.16E-48 | -1.69E-46 |
| MS.gene052133 | MS.gene057287 | 0.802108 | 6.56E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene057287 | 0.802009 | 6.88E-49 | -1.69E-46 |
| MS.gene053678 | MS.gene057287 | 0.804386 | 2.21E-49 | -1.69E-46 |
| MS.gene055468 | MS.gene057287 | 0.805021 | 1.63E-49 | -1.69E-46 |
| MS.gene057287 | MS.gene060587 | 0.800525 | 1.38E-48 | -1.69E-46 |
| MS.gene057287 | MS.gene061296 | 0.816894 | 4.37E-52 | -1.69E-46 |
| MS.gene057287 | MS.gene062042 | 0.811073 | 8.40E-51 | -1.69E-46 |
| MS.gene057287 | MS.gene062743 | 0.802369 | 5.80E-49 | -1.69E-46 |
| MS.gene057287 | MS.gene063086 | 0.81517 | 1.06E-51 | -1.69E-46 |
| MS.gene057287 | MS.gene068924 | 0.814729 | 1.33E-51 | -1.69E-46 |
| MS.gene057287 | MS.gene071301 | 0.817089 | 3.95E-52 | -1.69E-46 |
| MS.gene057287 | MS.gene072150 | 0.80499 | 1.66E-49 | -1.69E-46 |
| MS.gene057287 | MS.gene073083 | 0.818003 | 2.46E-52 | -1.69E-46 |
| MS.gene057287 | MS.gene07388 | 0.81153 | 6.69E-51 | -1.69E-46 |
| MS.gene057287 | MS.gene074258 | 0.833509 | 5.12E-56 | -1.69E-46 |
| MS.gene057287 | MS.gene20302 | 0.811983 | 5.33E-51 | -1.69E-46 |
| MS.gene057287 | MS.gene22499 | 0.806946 | 6.43E-50 | -1.69E-46 |
| MS.gene057287 | MS.gene24488 | 0.840589 | 7.94E-58 | -1.69E-46 |
| MS.gene057287 | MS.gene25435 | 0.808902 | 2.46E-50 | -1.69E-46 |
| MS.gene057287 | MS.gene25436 | 0.801801 | 7.58E-49 | -1.69E-46 |
| MS.gene057287 | MS.gene26832 | 0.817706 | 2.87E-52 | -1.69E-46 |
| MS.gene057287 | MS.gene27482 | 0.806356 | 8.56E-50 | -1.69E-46 |
| MS.gene057287 | MS.gene29807 | 0.819086 | 1.40E-52 | -1.69E-46 |
| MS.gene057287 | MS.gene29920 | 0.805064 | 1.60E-49 | -1.69E-46 |
| MS.gene057287 | MS.gene29921 | 0.804569 | 2.03E-49 | -1.69E-46 |
| MS.gene057287 | MS.gene31802 | 0.80794 | 3.95E-50 | -1.69E-46 |
| MS.gene057287 | MS.gene35438 | 0.800447 | 1.44E-48 | -1.69E-46 |
| MS.gene057287 | MS.gene35575 | 0.810133 | 1.34E-50 | -1.69E-46 |
| MS.gene057287 | MS.gene36215 | 0.809296 | 2.03E-50 | -1.69E-46 |
| MS.gene057287 | MS.gene36568 | 0.811876 | 5.62E-51 | -1.69E-46 |
| MS.gene057287 | MS.gene37023 | 0.802317 | 5.94E-49 | -1.69E-46 |
| MS.gene057287 | MS.gene38844 | 0.82649 | 2.64E-54 | -1.69E-46 |
| MS.gene057287 | MS.gene39716 | 0.817272 | 3.59E-52 | -1.69E-46 |
| MS.gene057287 | MS.gene40591 | 0.825946 | 3.55E-54 | -1.69E-46 |
| MS.gene057287 | MS.gene40595 | 0.810825 | 9.51E-51 | -1.69E-46 |
| MS.gene057287 | MS.gene42162 | 0.807584 | 4.71E-50 | -1.69E-46 |
| MS.gene057287 | MS.gene43075 | 0.821376 | 4.17E-53 | -1.69E-46 |
| MS.gene057287 | MS.gene43183 | 0.808839 | 2.54E-50 | -1.69E-46 |
| MS.gene057287 | MS.gene43537 | 0.81059 | 1.07E-50 | -1.69E-46 |
| MS.gene057287 | MS.gene44141 | 0.80097 | 1.12E-48 | -1.69E-46 |
| MS.gene057287 | MS.gene44631 | 0.802448 | 5.58E-49 | -1.69E-46 |
| MS.gene057287 | MS.gene45425 | 0.803435 | 3.49E-49 | -1.69E-46 |
| MS.gene057287 | MS.gene50775 | 0.817654 | 2.95E-52 | -1.69E-46 |
| MS.gene057287 | MS.gene52075 | 0.800964 | 1.13E-48 | -1.69E-46 |
| MS.gene057287 | MS.gene52946 | 0.801071 | 1.07E-48 | -1.69E-46 |
| MS.gene057287 | MS.gene56462 | 0.842444 | 2.58E-58 | -1.69E-46 |
| MS.gene057287 | MS.gene56646 | 0.802125 | 6.51E-49 | -1.69E-46 |
| MS.gene057287 | MS.gene59676 | 0.811729 | 6.05E-51 | -1.69E-46 |
| MS.gene057287 | MS.gene61133 | 0.805685 | 1.18E-49 | -1.69E-46 |
| MS.gene057287 | MS.gene62009 | 0.805917 | 1.06E-49 | -1.69E-46 |
| MS.gene057287 | MS.gene63032 | 0.804476 | 2.12E-49 | -1.69E-46 |
| MS.gene057287 | MS.gene64349 | 0.805106 | 1.57E-49 | -1.69E-46 |
| MS.gene057287 | MS.gene68663 | 0.809113 | 2.22E-50 | -1.69E-46 |
| MS.gene057287 | MS.gene68824 | 0.812683 | 3.75E-51 | -1.69E-46 |
| MS.gene057287 | MS.gene72121 | 0.800441 | 1.44E-48 | -1.69E-46 |
| MS.gene057287 | MS.gene75990 | 0.815154 | 1.07E-51 | -1.69E-46 |
| MS.gene057287 | MS.gene76034 | 0.818731 | 1.68E-52 | -1.69E-46 |
| MS.gene057287 | MS.gene79960 | 0.803681 | 3.10E-49 | -1.69E-46 |
| MS.gene057287 | MS.gene80257 | 0.836185 | 1.09E-56 | -1.69E-46 |
| MS.gene057287 | MS.gene83996 | 0.810548 | 1.09E-50 | -1.69E-46 |
| MS.gene057287 | MS.gene84469 | 0.870827 | 1.09E-66 | -1.69E-46 |
| MS.gene057287 | MS.gene84847 | 0.858716 | 6.81E-63 | -1.69E-46 |
| MS.gene057287 | MS.gene86716 | 0.813999 | 1.93E-51 | -1.69E-46 |
| MS.gene057287 | MS.gene89562 | 0.81377 | 2.16E-51 | -1.69E-46 |
| MS.gene057287 | MS.gene90646 | 0.806713 | 7.20E-50 | -1.69E-46 |
| MS.gene057287 | MS.gene96526 | 0.80961 | 1.74E-50 | -1.69E-46 |
| MS.gene057287 | MS.gene97236 | 0.818771 | 1.65E-52 | -1.69E-46 |
| MS.gene057287 | MS.gene97237 | 0.872291 | 3.56E-67 | -1.69E-46 |
| MS.gene057287 | MS.gene97238 | 0.89376 | 4.66E-75 | -1.69E-46 |
| MS.gene057287 | MS.gene98394 | 0.812037 | 5.19E-51 | -1.69E-46 |
| MS.gene057287 | MS.gene99550 | 0.814368 | 1.60E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057287.t1 | MTR_1g041155 | 81.111 | 360 | 10 | 5 | 1 | 305 | 1 | 357 | 0.0 | 549 |
| MS.gene057287.t1 | MTR_1g076500 | 65.833 | 240 | 72 | 5 | 69 | 298 | 142 | 381 | 9.11e-98 | 293 |
| MS.gene057287.t1 | MTR_7g095960 | 41.257 | 366 | 137 | 10 | 4 | 305 | 5 | 356 | 1.86e-77 | 240 |
| MS.gene057287.t1 | MTR_1g070970 | 54.237 | 236 | 83 | 6 | 70 | 303 | 136 | 348 | 1.51e-68 | 217 |
| MS.gene057287.t1 | MTR_8g093440 | 40.845 | 142 | 77 | 1 | 102 | 236 | 20 | 161 | 3.02e-37 | 132 |
| MS.gene057287.t1 | MTR_8g093440 | 40.845 | 142 | 77 | 1 | 102 | 236 | 82 | 223 | 1.12e-36 | 132 |
| MS.gene057287.t1 | MTR_7g083080 | 46.875 | 128 | 62 | 2 | 101 | 222 | 129 | 256 | 1.87e-35 | 130 |
| MS.gene057287.t1 | MTR_4g132963 | 44.068 | 118 | 66 | 0 | 104 | 221 | 117 | 234 | 1.19e-34 | 127 |
| MS.gene057287.t1 | MTR_4g132940 | 44.068 | 118 | 66 | 0 | 104 | 221 | 117 | 234 | 1.19e-34 | 127 |
| MS.gene057287.t1 | MTR_4g132970 | 39.850 | 133 | 80 | 0 | 104 | 236 | 138 | 270 | 2.08e-34 | 127 |
| MS.gene057287.t1 | MTR_2g025520 | 42.754 | 138 | 75 | 1 | 110 | 247 | 142 | 275 | 4.67e-33 | 124 |
| MS.gene057287.t1 | MTR_2g013780 | 40.945 | 127 | 69 | 2 | 102 | 222 | 143 | 269 | 2.87e-32 | 122 |
| MS.gene057287.t1 | MTR_4g078310 | 38.889 | 126 | 68 | 2 | 102 | 219 | 119 | 243 | 6.82e-30 | 115 |
| MS.gene057287.t1 | MTR_4g088770 | 36.220 | 127 | 75 | 2 | 102 | 222 | 149 | 275 | 3.09e-29 | 114 |
| MS.gene057287.t1 | MTR_4g088760 | 36.220 | 127 | 75 | 2 | 102 | 222 | 202 | 328 | 9.48e-29 | 114 |
| MS.gene057287.t1 | MTR_2g013880 | 30.189 | 159 | 105 | 2 | 75 | 227 | 116 | 274 | 3.35e-27 | 108 |
| MS.gene057287.t1 | MTR_7g053310 | 37.143 | 140 | 76 | 3 | 90 | 223 | 114 | 247 | 3.96e-27 | 107 |
| MS.gene057287.t1 | MTR_8g012240 | 35.556 | 135 | 74 | 4 | 95 | 222 | 130 | 258 | 3.64e-25 | 102 |
| MS.gene057287.t1 | MTR_5g008720 | 34.677 | 124 | 75 | 1 | 102 | 219 | 97 | 220 | 3.59e-24 | 99.0 |
| MS.gene057287.t1 | MTR_1g103610 | 85.294 | 34 | 5 | 0 | 204 | 237 | 1 | 34 | 8.70e-14 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057287.t1 | AT3G22970 | 44.386 | 383 | 115 | 11 | 4 | 305 | 5 | 370 | 2.55e-84 | 258 |
| MS.gene057287.t1 | AT4G14620 | 45.480 | 354 | 123 | 10 | 4 | 303 | 2 | 339 | 1.50e-77 | 240 |
| MS.gene057287.t1 | AT3G22970 | 52.400 | 250 | 91 | 5 | 69 | 305 | 25 | 259 | 5.17e-76 | 233 |
| MS.gene057287.t1 | AT2G38820 | 40.190 | 316 | 114 | 8 | 4 | 255 | 5 | 309 | 2.64e-68 | 215 |
| MS.gene057287.t1 | AT3G54550 | 36.462 | 277 | 101 | 9 | 5 | 221 | 6 | 267 | 6.21e-49 | 165 |
| MS.gene057287.t1 | AT3G07350 | 39.735 | 151 | 79 | 2 | 95 | 239 | 134 | 278 | 2.56e-35 | 129 |
| MS.gene057287.t1 | AT2G39650 | 42.742 | 124 | 65 | 1 | 106 | 223 | 52 | 175 | 5.61e-32 | 119 |
| MS.gene057287.t1 | AT2G39650 | 42.742 | 124 | 65 | 1 | 106 | 223 | 114 | 237 | 1.76e-31 | 119 |
| MS.gene057287.t1 | AT3G25240 | 38.012 | 171 | 97 | 3 | 61 | 222 | 72 | 242 | 5.93e-31 | 117 |
| MS.gene057287.t1 | AT4G32480 | 40.496 | 121 | 66 | 2 | 106 | 220 | 120 | 240 | 6.35e-28 | 109 |
| MS.gene057287.t1 | AT1G77145 | 35.616 | 146 | 76 | 2 | 99 | 226 | 72 | 217 | 5.94e-27 | 106 |
| MS.gene057287.t1 | AT1G77160 | 35.616 | 146 | 76 | 2 | 99 | 226 | 22 | 167 | 8.05e-27 | 104 |
| MS.gene057287.t1 | AT1G77160 | 35.616 | 146 | 76 | 2 | 99 | 226 | 77 | 222 | 1.36e-26 | 105 |
| MS.gene057287.t1 | AT2G20670 | 39.823 | 113 | 62 | 1 | 114 | 220 | 130 | 242 | 1.44e-26 | 106 |
| MS.gene057287.t1 | AT1G62420 | 37.391 | 115 | 69 | 1 | 110 | 221 | 116 | 230 | 8.80e-26 | 103 |
| MS.gene057287.t1 | AT1G12030 | 38.053 | 113 | 69 | 1 | 110 | 221 | 118 | 230 | 1.01e-24 | 101 |
| MS.gene057287.t1 | AT1G77145 | 34.400 | 125 | 72 | 1 | 135 | 249 | 59 | 183 | 3.28e-21 | 89.7 |
| MS.gene057287.t1 | AT2G38820 | 33.158 | 190 | 63 | 5 | 4 | 133 | 5 | 190 | 1.92e-18 | 82.4 |
Find 73 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTGTTTGCTTGGCGAAAA+TGG | 0.121680 | 1.4:-25559360 | MS.gene057287:intron |
| CATCCATTTGCAAATCAAAA+TGG | 0.188560 | 1.4:-25558938 | MS.gene057287:CDS |
| TCTTTAGTGGTATTGTTCTT+AGG | 0.254266 | 1.4:+25559419 | None:intergenic |
| TTCAACCTCCACTTCGAAAC+TGG | 0.330861 | 1.4:+25559470 | None:intergenic |
| GCCGCATCGGAGACTTGCTT+TGG | 0.348894 | 1.4:+25558526 | None:intergenic |
| TCGGAGGTGGATAGTGATTC+TGG | 0.369464 | 1.4:-25558484 | MS.gene057287:CDS |
| GAGTCCAGCGTGGCAGTTAC+CGG | 0.378357 | 1.4:-25558383 | MS.gene057287:CDS |
| GTGGTATTGTTCTTAGGAAA+AGG | 0.405068 | 1.4:+25559425 | None:intergenic |
| GCTGAAAAGCTTCAATCTCT+TGG | 0.409442 | 1.4:-25558969 | MS.gene057287:CDS |
| ATCCATTTGCAAATCAAAAT+GGG | 0.412073 | 1.4:-25558937 | MS.gene057287:CDS |
| TTCTCTGAATCTATGTCAAT+CGG | 0.419113 | 1.4:+25559521 | None:intergenic |
| ATTCCTCTTTCACCACTCTT+CGG | 0.422133 | 1.4:+25558352 | None:intergenic |
| ACCACCACCACCATCTTTAG+TGG | 0.439948 | 1.4:+25559406 | None:intergenic |
| AGTCTCCGATGCGGCGGCGG+TGG | 0.440423 | 1.4:-25558518 | MS.gene057287:CDS |
| GGATAGTGATTCTGGTGAAT+TGG | 0.451879 | 1.4:-25558476 | MS.gene057287:CDS |
| TTCGCCAAGCAAACAGAGCT+TGG | 0.466708 | 1.4:+25559365 | None:intergenic |
| GGAACCAAGCTCTGTTTGCT+TGG | 0.476354 | 1.4:-25559369 | MS.gene057287:CDS |
| AGAGAAAGCCACTACGGAAC+TGG | 0.478102 | 1.4:-25559504 | MS.gene057287:CDS |
| TGGTGGTGGAGTTGCTGAGT+TGG | 0.486575 | 1.4:-25559390 | MS.gene057287:CDS |
| CCACTCTTCGGTTTCACCGC+CGG | 0.488504 | 1.4:+25558364 | None:intergenic |
| GCAAGTCTCCGATGCGGCGG+CGG | 0.496129 | 1.4:-25558521 | MS.gene057287:CDS |
| CGTCGCGGTCGTGTCGGAGG+CGG | 0.498858 | 1.4:-25558644 | MS.gene057287:CDS |
| AAGAACAATACCACTAAAGA+TGG | 0.502600 | 1.4:-25559416 | MS.gene057287:CDS |
| GAGTGGTGAAAGAGGAATGA+AGG | 0.505285 | 1.4:-25558347 | MS.gene057287:CDS |
| TGGATTAGCTTCTTTGCTGA+AGG | 0.511501 | 1.4:-25558320 | MS.gene057287:CDS |
| GGAGAAAGCTTCGCCGGAGG+AGG | 0.512134 | 1.4:-25558437 | MS.gene057287:CDS |
| TTCGCCGGAGGAGGTAGTTA+CGG | 0.512281 | 1.4:-25558428 | MS.gene057287:CDS |
| GCGATTACATGTTAGCGAAA+TGG | 0.512607 | 1.4:-25558570 | MS.gene057287:CDS |
| TGCTTTGGCCTAGCTGCCGA+CGG | 0.512808 | 1.4:+25558541 | None:intergenic |
| AAGCAGAGTTTGAAGAAGAA+AGG | 0.517627 | 1.4:-25558619 | MS.gene057287:CDS |
| AAAACAAATTCATGTCCTGC+TGG | 0.520750 | 1.4:-25558912 | MS.gene057287:intron |
| ACCGCCGGTAACTGCCACGC+TGG | 0.521213 | 1.4:+25558379 | None:intergenic |
| TGTTTCCACCGCCGCCGCAT+CGG | 0.530760 | 1.4:+25558513 | None:intergenic |
| GGAAACAGTTTCATCGGAGG+TGG | 0.532775 | 1.4:-25558497 | MS.gene057287:CDS |
| ACTAAAGATGGTGGTGGTGG+TGG | 0.532828 | 1.4:-25559404 | MS.gene057287:CDS |
| GCAGATCGTCGCGGTCGTGT+CGG | 0.535759 | 1.4:-25558650 | MS.gene057287:CDS |
| GGATCTCTCCGTCGGCAGCT+AGG | 0.537066 | 1.4:-25558549 | MS.gene057287:CDS |
| CTGAATCTATGTCAATCGGT+TGG | 0.551079 | 1.4:+25559525 | None:intergenic |
| TCAATTCATTGATGCGATTG+TGG | 0.553988 | 1.4:-25558800 | MS.gene057287:CDS |
| TGGTGAGGAGAAAGCTTCGC+CGG | 0.554242 | 1.4:-25558443 | MS.gene057287:CDS |
| AAACCGAAGAGTGGTGAAAG+AGG | 0.554832 | 1.4:-25558355 | MS.gene057287:CDS |
| GAAAGAGGAATGAAGGTGAC+TGG | 0.556629 | 1.4:-25558340 | MS.gene057287:CDS |
| TTTCGAAGTGGAGGTTGAAG+AGG | 0.556890 | 1.4:-25559466 | MS.gene057287:CDS |
| GGCGGTGGAAACAGTTTCAT+CGG | 0.562645 | 1.4:-25558503 | MS.gene057287:CDS |
| CGTTCTTCAACACTTACACA+AGG | 0.565445 | 1.4:+25559074 | None:intergenic |
| CCGCGACGATCTGCTTCAAA+CGG | 0.566237 | 1.4:+25558659 | None:intergenic |
| CTTGTGTAAGTGTTGAAGAA+CGG | 0.570166 | 1.4:-25559073 | MS.gene057287:CDS |
| ATGTAATCGCGCTTCCTCCA+CGG | 0.582747 | 1.4:+25558583 | None:intergenic |
| TTCATTGATGCGATTGTGGA+AGG | 0.586622 | 1.4:-25558796 | MS.gene057287:CDS |
| GATCGTCGCGGTCGTGTCGG+AGG | 0.593085 | 1.4:-25558647 | MS.gene057287:CDS |
| ATGAACCAGTTTCGAAGTGG+AGG | 0.593261 | 1.4:-25559475 | MS.gene057287:CDS |
| ACCACTAAAGATGGTGGTGG+TGG | 0.595959 | 1.4:-25559407 | MS.gene057287:CDS |
| GCCGCCGTAACTACCTCCTC+CGG | 0.598847 | 1.4:+25558424 | None:intergenic |
| ATTTCGAACCAGTTCCGTAG+TGG | 0.603877 | 1.4:+25559496 | None:intergenic |
| AGATTCAGAGAAAGCCACTA+CGG | 0.617727 | 1.4:-25559510 | MS.gene057287:CDS |
| AAAGCAAGTCTCCGATGCGG+CGG | 0.623703 | 1.4:-25558524 | MS.gene057287:CDS |
| GAATGCATGTTCCGCCGTGG+AGG | 0.625206 | 1.4:-25558597 | MS.gene057287:CDS |
| GAAATGAACCAGTTTCGAAG+TGG | 0.634899 | 1.4:-25559478 | MS.gene057287:CDS |
| AACAATACCACTAAAGATGG+TGG | 0.638792 | 1.4:-25559413 | MS.gene057287:CDS |
| AGCGAAATGGATCTCTCCGT+CGG | 0.639822 | 1.4:-25558557 | MS.gene057287:CDS |
| TCAATCGGTTGGATCTTCAA+CGG | 0.649259 | 1.4:+25559536 | None:intergenic |
| TCCAGCGTGGCAGTTACCGG+CGG | 0.657779 | 1.4:-25558380 | MS.gene057287:CDS |
| CCGGCGGTGAAACCGAAGAG+TGG | 0.661349 | 1.4:-25558364 | MS.gene057287:CDS |
| AATACCACTAAAGATGGTGG+TGG | 0.666485 | 1.4:-25559410 | MS.gene057287:CDS |
| AAGGAATGCATGTTCCGCCG+TGG | 0.669326 | 1.4:-25558600 | MS.gene057287:CDS |
| GGCGGCGACAGCGACAGCGA+CGG | 0.672784 | 1.4:-25558407 | MS.gene057287:CDS |
| GCCGGAGGAGGTAGTTACGG+CGG | 0.688861 | 1.4:-25558425 | MS.gene057287:CDS |
| GGTGGAAACAGTTTCATCGG+AGG | 0.697111 | 1.4:-25558500 | MS.gene057287:CDS |
| TGAGGAGAAAGCTTCGCCGG+AGG | 0.709522 | 1.4:-25558440 | MS.gene057287:CDS |
| GCCAAAGCAAGTCTCCGATG+CGG | 0.718766 | 1.4:-25558527 | MS.gene057287:CDS |
| CCGTTTGAAGCAGATCGTCG+CGG | 0.731932 | 1.4:-25558659 | MS.gene057287:CDS |
| CAGCGACGGTGAGTCCAGCG+TGG | 0.734694 | 1.4:-25558393 | MS.gene057287:CDS |
| TAATCGCGCTTCCTCCACGG+CGG | 0.770748 | 1.4:+25558586 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCGTTTTTTGTTTTGTTTT+AGG | - | chr1.4:25559022-25559041 | MS.gene057287:CDS | 20.0% |
| ! | ATCCATTTGCAAATCAAAAT+GGG | - | chr1.4:25558914-25558933 | MS.gene057287:CDS | 25.0% |
| !!! | TATTGATATCGATTTTCGAT+CGG | - | chr1.4:25559090-25559109 | MS.gene057287:intron | 25.0% |
| AAGAACAATACCACTAAAGA+TGG | - | chr1.4:25558435-25558454 | MS.gene057287:CDS | 30.0% | |
| CATCCATTTGCAAATCAAAA+TGG | - | chr1.4:25558913-25558932 | MS.gene057287:CDS | 30.0% | |
| GAAATCGAAATCATCTTCAT+CGG | + | chr1.4:25558601-25558620 | None:intergenic | 30.0% | |
| GAAGATGATTTCGATTTCAA+CGG | - | chr1.4:25558603-25558622 | MS.gene057287:CDS | 30.0% | |
| TCTTTAGTGGTATTGTTCTT+AGG | + | chr1.4:25558435-25558454 | None:intergenic | 30.0% | |
| TTAGAGAATTAATTACCAGC+AGG | + | chr1.4:25558957-25558976 | None:intergenic | 30.0% | |
| TTCTCTGAATCTATGTCAAT+CGG | + | chr1.4:25558333-25558352 | None:intergenic | 30.0% | |
| ! | TTTTTGCCGACGAAAATATA+CGG | + | chr1.4:25559169-25559188 | None:intergenic | 30.0% |
| AAAACAAATTCATGTCCTGC+TGG | - | chr1.4:25558939-25558958 | MS.gene057287:CDS | 35.0% | |
| AACAATACCACTAAAGATGG+TGG | - | chr1.4:25558438-25558457 | MS.gene057287:CDS | 35.0% | |
| AAGCAGAGTTTGAAGAAGAA+AGG | - | chr1.4:25559232-25559251 | MS.gene057287:intron | 35.0% | |
| GAAAATGGTTCAGAGTTTCA+TGG | - | chr1.4:25558506-25558525 | MS.gene057287:CDS | 35.0% | |
| GTGGTATTGTTCTTAGGAAA+AGG | + | chr1.4:25558429-25558448 | None:intergenic | 35.0% | |
| TCAATTCATTGATGCGATTG+TGG | - | chr1.4:25559051-25559070 | MS.gene057287:CDS | 35.0% | |
| ! | GTATATTTTCGTCGGCAAAA+AGG | - | chr1.4:25559168-25559187 | MS.gene057287:intron | 35.0% |
| ! | TCACTTCCGTATATTTTCGT+CGG | - | chr1.4:25559160-25559179 | MS.gene057287:intron | 35.0% |
| !! | CTTGTGTAAGTGTTGAAGAA+CGG | - | chr1.4:25558778-25558797 | MS.gene057287:CDS | 35.0% |
| !!! | CTCCCATTTTGATTTGCAAA+TGG | + | chr1.4:25558919-25558938 | None:intergenic | 35.0% |
| AGATTCAGAGAAAGCCACTA+CGG | - | chr1.4:25558341-25558360 | MS.gene057287:CDS | 40.0% | |
| ATTCCTCTTTCACCACTCTT+CGG | + | chr1.4:25559502-25559521 | None:intergenic | 40.0% | |
| CGTTCTTCAACACTTACACA+AGG | + | chr1.4:25558780-25558799 | None:intergenic | 40.0% | |
| CTGAATCTATGTCAATCGGT+TGG | + | chr1.4:25558329-25558348 | None:intergenic | 40.0% | |
| CTTGAGTGAGTCAGTTGAAT+CGG | + | chr1.4:25558649-25558668 | None:intergenic | 40.0% | |
| GAAATGAACCAGTTTCGAAG+TGG | - | chr1.4:25558373-25558392 | MS.gene057287:CDS | 40.0% | |
| GCGATTACATGTTAGCGAAA+TGG | - | chr1.4:25559281-25559300 | MS.gene057287:intron | 40.0% | |
| GCTGAAAAGCTTCAATCTCT+TGG | - | chr1.4:25558882-25558901 | MS.gene057287:intron | 40.0% | |
| GGATAGTGATTCTGGTGAAT+TGG | - | chr1.4:25559375-25559394 | MS.gene057287:CDS | 40.0% | |
| TCAATCGGTTGGATCTTCAA+CGG | + | chr1.4:25558318-25558337 | None:intergenic | 40.0% | |
| TTCATTGATGCGATTGTGGA+AGG | - | chr1.4:25559055-25559074 | MS.gene057287:CDS | 40.0% | |
| ! | AATACCACTAAAGATGGTGG+TGG | - | chr1.4:25558441-25558460 | MS.gene057287:CDS | 40.0% |
| !! | GGTGAATTGGAGCTGATTTT+TGG | - | chr1.4:25559388-25559407 | MS.gene057287:CDS | 40.0% |
| !! | TGGATTAGCTTCTTTGCTGA+AGG | - | chr1.4:25559531-25559550 | MS.gene057287:CDS | 40.0% |
| !!! | ATTGGAGCTGATTTTTGGTG+AGG | - | chr1.4:25559393-25559412 | MS.gene057287:CDS | 40.0% |
| AAACCGAAGAGTGGTGAAAG+AGG | - | chr1.4:25559496-25559515 | MS.gene057287:CDS | 45.0% | |
| ATGAACCAGTTTCGAAGTGG+AGG | - | chr1.4:25558376-25558395 | MS.gene057287:CDS | 45.0% | |
| ATTTCGAACCAGTTCCGTAG+TGG | + | chr1.4:25558358-25558377 | None:intergenic | 45.0% | |
| CTCTGTTTGCTTGGCGAAAA+TGG | - | chr1.4:25558491-25558510 | MS.gene057287:CDS | 45.0% | |
| GAAAGAGGAATGAAGGTGAC+TGG | - | chr1.4:25559511-25559530 | MS.gene057287:CDS | 45.0% | |
| GAGTGGTGAAAGAGGAATGA+AGG | - | chr1.4:25559504-25559523 | MS.gene057287:CDS | 45.0% | |
| TTCAACCTCCACTTCGAAAC+TGG | + | chr1.4:25558384-25558403 | None:intergenic | 45.0% | |
| TTTCGAAGTGGAGGTTGAAG+AGG | - | chr1.4:25558385-25558404 | MS.gene057287:CDS | 45.0% | |
| ! | CGATTCAACTGACTCACTCA+AGG | - | chr1.4:25558647-25558666 | MS.gene057287:CDS | 45.0% |
| ! | TAGCGTTGAAGCAGTTACAG+CGG | + | chr1.4:25558572-25558591 | None:intergenic | 45.0% |
| ACCACCACCACCATCTTTAG+TGG | + | chr1.4:25558448-25558467 | None:intergenic | 50.0% | |
| AGAGAAAGCCACTACGGAAC+TGG | - | chr1.4:25558347-25558366 | MS.gene057287:CDS | 50.0% | |
| AGCGAAATGGATCTCTCCGT+CGG | - | chr1.4:25559294-25559313 | MS.gene057287:intron | 50.0% | |
| ATGTAATCGCGCTTCCTCCA+CGG | + | chr1.4:25559271-25559290 | None:intergenic | 50.0% | |
| GAGTGAGTCAGTTGAATCGG+TGG | + | chr1.4:25558646-25558665 | None:intergenic | 50.0% | |
| GGAAACAGTTTCATCGGAGG+TGG | - | chr1.4:25559354-25559373 | MS.gene057287:intron | 50.0% | |
| GGAACCAAGCTCTGTTTGCT+TGG | - | chr1.4:25558482-25558501 | MS.gene057287:CDS | 50.0% | |
| GGCGGTGGAAACAGTTTCAT+CGG | - | chr1.4:25559348-25559367 | MS.gene057287:intron | 50.0% | |
| GGTGGAAACAGTTTCATCGG+AGG | - | chr1.4:25559351-25559370 | MS.gene057287:intron | 50.0% | |
| TCGGAGGTGGATAGTGATTC+TGG | - | chr1.4:25559367-25559386 | MS.gene057287:CDS | 50.0% | |
| TTCGCCAAGCAAACAGAGCT+TGG | + | chr1.4:25558489-25558508 | None:intergenic | 50.0% | |
| !! | ACCACTAAAGATGGTGGTGG+TGG | - | chr1.4:25558444-25558463 | MS.gene057287:CDS | 50.0% |
| !! | ACTAAAGATGGTGGTGGTGG+TGG | - | chr1.4:25558447-25558466 | MS.gene057287:CDS | 50.0% |
| AAAGCAAGTCTCCGATGCGG+CGG | - | chr1.4:25559327-25559346 | MS.gene057287:intron | 55.0% | |
| AAGGAATGCATGTTCCGCCG+TGG | - | chr1.4:25559251-25559270 | MS.gene057287:intron | 55.0% | |
| CAGTTACAGCGGTTGCGAGA+CGG | + | chr1.4:25558561-25558580 | None:intergenic | 55.0% | |
| CCGCGACGATCTGCTTCAAA+CGG | + | chr1.4:25559195-25559214 | None:intergenic | 55.0% | |
| CGAGACGGTGGTTGAAGTTG+CGG | + | chr1.4:25558546-25558565 | None:intergenic | 55.0% | |
| GCCAAAGCAAGTCTCCGATG+CGG | - | chr1.4:25559324-25559343 | MS.gene057287:intron | 55.0% | |
| GGTGGTTGAAGTTGCGGTTG+CGG | + | chr1.4:25558540-25558559 | None:intergenic | 55.0% | |
| TGGTGAGGAGAAAGCTTCGC+CGG | - | chr1.4:25559408-25559427 | MS.gene057287:CDS | 55.0% | |
| TTCGCCGGAGGAGGTAGTTA+CGG | - | chr1.4:25559423-25559442 | MS.gene057287:CDS | 55.0% | |
| ! | CCGTTTGAAGCAGATCGTCG+CGG | - | chr1.4:25559192-25559211 | MS.gene057287:intron | 55.0% |
| !! | TGGTGGTGGAGTTGCTGAGT+TGG | - | chr1.4:25558461-25558480 | MS.gene057287:CDS | 55.0% |
| CCACTCTTCGGTTTCACCGC+CGG | + | chr1.4:25559490-25559509 | None:intergenic | 60.0% | |
| GAATGCATGTTCCGCCGTGG+AGG | - | chr1.4:25559254-25559273 | MS.gene057287:intron | 60.0% | |
| GAGTCCAGCGTGGCAGTTAC+CGG | - | chr1.4:25559468-25559487 | MS.gene057287:CDS | 60.0% | |
| TAATCGCGCTTCCTCCACGG+CGG | + | chr1.4:25559268-25559287 | None:intergenic | 60.0% | |
| TGAGGAGAAAGCTTCGCCGG+AGG | - | chr1.4:25559411-25559430 | MS.gene057287:CDS | 60.0% | |
| TGCTTTGGCCTAGCTGCCGA+CGG | + | chr1.4:25559313-25559332 | None:intergenic | 60.0% | |
| TTACAGCGGTTGCGAGACGG+TGG | + | chr1.4:25558558-25558577 | None:intergenic | 60.0% | |
| ! | GCCGCATCGGAGACTTGCTT+TGG | + | chr1.4:25559328-25559347 | None:intergenic | 60.0% |
| CCGGCGGTGAAACCGAAGAG+TGG | - | chr1.4:25559487-25559506 | MS.gene057287:CDS | 65.0% | |
| GCAGATCGTCGCGGTCGTGT+CGG | - | chr1.4:25559201-25559220 | MS.gene057287:intron | 65.0% | |
| GCCGCCGTAACTACCTCCTC+CGG | + | chr1.4:25559430-25559449 | None:intergenic | 65.0% | |
| GCCGGAGGAGGTAGTTACGG+CGG | - | chr1.4:25559426-25559445 | MS.gene057287:CDS | 65.0% | |
| GGAGAAAGCTTCGCCGGAGG+AGG | - | chr1.4:25559414-25559433 | MS.gene057287:CDS | 65.0% | |
| GGATCTCTCCGTCGGCAGCT+AGG | - | chr1.4:25559302-25559321 | MS.gene057287:intron | 65.0% | |
| TCCAGCGTGGCAGTTACCGG+CGG | - | chr1.4:25559471-25559490 | MS.gene057287:CDS | 65.0% | |
| TGTTTCCACCGCCGCCGCAT+CGG | + | chr1.4:25559341-25559360 | None:intergenic | 65.0% | |
| ACCGCCGGTAACTGCCACGC+TGG | + | chr1.4:25559475-25559494 | None:intergenic | 70.0% | |
| CAGCGACGGTGAGTCCAGCG+TGG | - | chr1.4:25559458-25559477 | MS.gene057287:CDS | 70.0% | |
| GATCGTCGCGGTCGTGTCGG+AGG | - | chr1.4:25559204-25559223 | MS.gene057287:intron | 70.0% | |
| GCAAGTCTCCGATGCGGCGG+CGG | - | chr1.4:25559330-25559349 | MS.gene057287:intron | 70.0% | |
| AGTCTCCGATGCGGCGGCGG+TGG | - | chr1.4:25559333-25559352 | MS.gene057287:intron | 75.0% | |
| CGTCGCGGTCGTGTCGGAGG+CGG | - | chr1.4:25559207-25559226 | MS.gene057287:intron | 75.0% | |
| GGCGGCGACAGCGACAGCGA+CGG | - | chr1.4:25559444-25559463 | MS.gene057287:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 25558309 | 25559564 | 25558309 | ID=MS.gene057287 |
| chr1.4 | mRNA | 25558309 | 25559564 | 25558309 | ID=MS.gene057287.t1;Parent=MS.gene057287 |
| chr1.4 | exon | 25559361 | 25559564 | 25559361 | ID=MS.gene057287.t1.exon1;Parent=MS.gene057287.t1 |
| chr1.4 | CDS | 25559361 | 25559564 | 25559361 | ID=cds.MS.gene057287.t1;Parent=MS.gene057287.t1 |
| chr1.4 | exon | 25558913 | 25559105 | 25558913 | ID=MS.gene057287.t1.exon2;Parent=MS.gene057287.t1 |
| chr1.4 | CDS | 25558913 | 25559105 | 25558913 | ID=cds.MS.gene057287.t1;Parent=MS.gene057287.t1 |
| chr1.4 | exon | 25558309 | 25558829 | 25558309 | ID=MS.gene057287.t1.exon3;Parent=MS.gene057287.t1 |
| chr1.4 | CDS | 25558309 | 25558829 | 25558309 | ID=cds.MS.gene057287.t1;Parent=MS.gene057287.t1 |
| Gene Sequence |
| Protein sequence |
