Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97244.t1 | AGC78837.1 | 93.8 | 259 | 16 | 0 | 26 | 284 | 1 | 259 | 5.50E-133 | 483.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97244.t1 | P05491 | 96.0 | 252 | 6 | 2 | 34 | 284 | 12 | 260 | 7.2e-134 | 478.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97244.t1 | R4IUC9 | 93.8 | 259 | 16 | 0 | 26 | 284 | 1 | 259 | 3.9e-133 | 483.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050945 | MS.gene97244 | -0.806358 | 8.55E-50 | -1.69E-46 |
| MS.gene053931 | MS.gene97244 | -0.807947 | 3.94E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97244.t1 | MTR_0212s0040 | 94.779 | 249 | 1 | 1 | 36 | 284 | 620 | 856 | 1.15e-166 | 484 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97244.t1 | ATMG00160 | 88.764 | 267 | 23 | 2 | 18 | 284 | 1 | 260 | 8.16e-172 | 475 |
| MS.gene97244.t1 | AT2G07695 | 73.148 | 108 | 22 | 2 | 18 | 125 | 1 | 101 | 1.36e-43 | 150 |
Find 0 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAAATCAGATCTCTATTT+CGG | - | 37479:7543-7562 | MS.gene97244:CDS | 20.0% |
| ! | TAAGCAAAGGATAATTAGTA+GGG | + | 37479:6909-6928 | None:intergenic | 25.0% |
| !! | AATAATACGTAGATGAGTTT+TGG | + | 37479:7449-7468 | None:intergenic | 25.0% |
| !! | ATTTGCTCTTTTATACTCAA+TGG | - | 37479:7231-7250 | MS.gene97244:CDS | 25.0% |
| AATCAATTAATCCCACAAAC+AGG | - | 37479:7682-7701 | MS.gene97244:CDS | 30.0% | |
| ATCAATTAATCCCACAAACA+GGG | - | 37479:7683-7702 | MS.gene97244:CDS | 30.0% | |
| CTAAGCAAAGGATAATTAGT+AGG | + | 37479:6910-6929 | None:intergenic | 30.0% | |
| CTAGTAAAGATTATGGTTCT+CGG | - | 37479:7653-7672 | MS.gene97244:CDS | 30.0% | |
| CTTGAAATCCTAATTGCAAT+GGG | + | 37479:6988-7007 | None:intergenic | 30.0% | |
| GAGTATAAAAGAGCAAATGA+TGG | + | 37479:7229-7248 | None:intergenic | 30.0% | |
| TAGTAAAGATTATGGTTCTC+GGG | - | 37479:7654-7673 | MS.gene97244:CDS | 30.0% | |
| TCAATCACGTTTATTAGAAG+TGG | - | 37479:7402-7421 | MS.gene97244:CDS | 30.0% | |
| TCTATTATTCCTTGCATCAT+AGG | + | 37479:7022-7041 | None:intergenic | 30.0% | |
| TCTTGAAATCCTAATTGCAA+TGG | + | 37479:6989-7008 | None:intergenic | 30.0% | |
| TGTTTGTGGGATTAATTGAT+TGG | + | 37479:7683-7702 | None:intergenic | 30.0% | |
| ! | TTTCTTCCTGATTCTGATTT+TGG | - | 37479:7060-7079 | MS.gene97244:CDS | 30.0% |
| ! | TTTTGACAGTTATACGATTC+CGG | - | 37479:7360-7379 | MS.gene97244:CDS | 30.0% |
| !!! | TGATTTTGGTTTTCGTATCA+CGG | - | 37479:7074-7093 | MS.gene97244:CDS | 30.0% |
| AAAAGTGAGTGACTGTTCAT+CGG | + | 37479:7344-7363 | None:intergenic | 35.0% | |
| ATGGTATAGCAATGAACATC+GGG | + | 37479:7210-7229 | None:intergenic | 35.0% | |
| ATTAGAAGTGGACAATAGAG+TGG | - | 37479:7414-7433 | MS.gene97244:CDS | 35.0% | |
| CGAAAACCAAAATCAGAATC+AGG | + | 37479:7069-7088 | None:intergenic | 35.0% | |
| CGAGAACCATAATCTTTACT+AGG | + | 37479:7655-7674 | None:intergenic | 35.0% | |
| CTATTGGACATCAATGGTAT+CGG | - | 37479:7290-7309 | MS.gene97244:CDS | 35.0% | |
| CTATTTCGGTACAAAGAGAA+GGG | - | 37479:7557-7576 | MS.gene97244:CDS | 35.0% | |
| GAACTACTATCGAGATTCTT+CGG | - | 37479:7164-7183 | MS.gene97244:CDS | 35.0% | |
| GATGGTATAGCAATGAACAT+CGG | + | 37479:7211-7230 | None:intergenic | 35.0% | |
| GCTGTTCCTAGTAAAGATTA+TGG | - | 37479:7646-7665 | MS.gene97244:CDS | 35.0% | |
| GGGAATTGAAGAAGATCAAT+CGG | + | 37479:6941-6960 | None:intergenic | 35.0% | |
| GTTCCATGAACAATTCTTTG+CGG | + | 37479:7148-7167 | None:intergenic | 35.0% | |
| TATTTCGGTACAAAGAGAAG+GGG | - | 37479:7558-7577 | MS.gene97244:CDS | 35.0% | |
| TCAAAGCTATTGGACATCAA+TGG | - | 37479:7284-7303 | MS.gene97244:CDS | 35.0% | |
| TCAATTAATCCCACAAACAG+GGG | - | 37479:7684-7703 | MS.gene97244:CDS | 35.0% | |
| TCTATTTCGGTACAAAGAGA+AGG | - | 37479:7556-7575 | MS.gene97244:CDS | 35.0% | |
| TTCCATGAACAATTCTTTGC+GGG | + | 37479:7147-7166 | None:intergenic | 35.0% | |
| ! | TCTTTTATACTCAATGGACG+AGG | - | 37479:7237-7256 | MS.gene97244:CDS | 35.0% |
| !! | TCCAATAGCTTTGATAGTCA+TGG | + | 37479:7278-7297 | None:intergenic | 35.0% |
| AGCATCACATTTGACACCTA+AGG | + | 37479:7512-7531 | None:intergenic | 40.0% | |
| ATCCCGCAAAGAATTGTTCA+TGG | - | 37479:7142-7161 | MS.gene97244:CDS | 40.0% | |
| ATGAACATCGGGATGATACT+AGG | + | 37479:7199-7218 | None:intergenic | 40.0% | |
| CAAAGAGAAGGGGTTTACTA+TGG | - | 37479:7568-7587 | MS.gene97244:CDS | 40.0% | |
| CATCCTGCGAAGAAAATCTT+GGG | + | 37479:6876-6895 | None:intergenic | 40.0% | |
| CCCAATTCTAGATCATCTTC+CGG | + | 37479:7382-7401 | None:intergenic | 40.0% | |
| CGGGATGATACTAGGAAATA+TGG | + | 37479:7191-7210 | None:intergenic | 40.0% | |
| GAACAATTCTTTGCGGGATT+GGG | + | 37479:7141-7160 | None:intergenic | 40.0% | |
| GAAGAAGATCAATCGGAAGA+AGG | + | 37479:6934-6953 | None:intergenic | 40.0% | |
| GAGATCTGATTTAAACGACC+AGG | + | 37479:7538-7557 | None:intergenic | 40.0% | |
| GCCATGACTATCAAAGCTAT+TGG | - | 37479:7274-7293 | MS.gene97244:CDS | 40.0% | |
| TCACATTTGACACCTAAGGA+AGG | + | 37479:7508-7527 | None:intergenic | 40.0% | |
| TGAACAATTCTTTGCGGGAT+TGG | + | 37479:7142-7161 | None:intergenic | 40.0% | |
| TGCTGATGTACCTCATAGTT+GGG | - | 37479:7477-7496 | MS.gene97244:CDS | 40.0% | |
| ! | ATACCCAAGATTTTCTTCGC+AGG | - | 37479:6870-6889 | MS.gene97244:CDS | 40.0% |
| ! | CCGGAAGATGATCTAGAATT+GGG | - | 37479:7379-7398 | MS.gene97244:CDS | 40.0% |
| ! | TCCGGAAGATGATCTAGAAT+TGG | - | 37479:7378-7397 | MS.gene97244:CDS | 40.0% |
| !! | ATACGTAGATGAGTTTTGGC+TGG | + | 37479:7445-7464 | None:intergenic | 40.0% |
| !! | ATAGCTTTGATAGTCATGGC+TGG | + | 37479:7274-7293 | None:intergenic | 40.0% |
| CTGCTGATGTACCTCATAGT+TGG | - | 37479:7476-7495 | MS.gene97244:CDS | 45.0% | |
| GCATCCTGCGAAGAAAATCT+TGG | + | 37479:6877-6896 | None:intergenic | 45.0% | |
| GTCAAATGTGATGCTGTACC+TGG | - | 37479:7517-7536 | MS.gene97244:CDS | 45.0% | |
| TCGGAAGAAGGACTAAGCAA+AGG | + | 37479:6922-6941 | None:intergenic | 45.0% | |
| TTCTACGACGATAGGCGTAA+AGG | + | 37479:7626-7645 | None:intergenic | 45.0% | |
| ! | GGTTTTCGTATCACGGATCT+TGG | - | 37479:7081-7100 | MS.gene97244:CDS | 45.0% |
| AGTTGGGCTGTACCTTCCTT+AGG | - | 37479:7493-7512 | MS.gene97244:CDS | 50.0% | |
| GCAGCAACACCTATGATGCA+AGG | - | 37479:7010-7029 | MS.gene97244:CDS | 50.0% | |
| GGAACAGCTTCTACGACGAT+AGG | + | 37479:7634-7653 | None:intergenic | 50.0% | |
| GGTCAGTGCAGTGAGATTTG+TGG | - | 37479:7589-7608 | MS.gene97244:CDS | 50.0% | |
| ! | GAAGGTACAGCCCAACTATG+AGG | + | 37479:7490-7509 | None:intergenic | 50.0% |
| CTGCGCTCTTTCTGATGCAG+CGG | - | 37479:6961-6980 | MS.gene97244:CDS | 55.0% | |
| GCAGCGGACCCATTGCAATT+AGG | - | 37479:6977-6996 | MS.gene97244:CDS | 55.0% | |
| ! | CTGCATCAGAAAGAGCGCAG+GGG | + | 37479:6961-6980 | None:intergenic | 55.0% |
| ! | GCTGCATCAGAAAGAGCGCA+GGG | + | 37479:6962-6981 | None:intergenic | 55.0% |
| ! | GGATCTTGGTTCGCGCTTTG+TGG | - | 37479:7095-7114 | MS.gene97244:CDS | 55.0% |
| ! | CGCTGCATCAGAAAGAGCGC+AGG | + | 37479:6963-6982 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 37479 | gene | 6860 | 7714 | 6860 | ID=MS.gene97244 |
| 37479 | mRNA | 6860 | 7714 | 6860 | ID=MS.gene97244.t1;Parent=MS.gene97244 |
| 37479 | exon | 6860 | 7714 | 6860 | ID=MS.gene97244.t1.exon1;Parent=MS.gene97244.t1 |
| 37479 | CDS | 6860 | 7714 | 6860 | ID=cds.MS.gene97244.t1;Parent=MS.gene97244.t1 |
| Gene Sequence |
| Protein sequence |
