Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97256.t1 | AGC78837.1 | 93.8 | 259 | 16 | 0 | 26 | 284 | 1 | 259 | 5.50E-133 | 483.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97256.t1 | P05491 | 96.0 | 252 | 6 | 2 | 34 | 284 | 12 | 260 | 7.2e-134 | 478.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97256.t1 | R4IUC9 | 93.8 | 259 | 16 | 0 | 26 | 284 | 1 | 259 | 3.9e-133 | 483.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050945 | MS.gene97256 | -0.811104 | 8.27E-51 | -1.69E-46 |
| MS.gene053931 | MS.gene97256 | -0.80538 | 1.37E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97256.t1 | MTR_0212s0040 | 94.779 | 249 | 1 | 1 | 36 | 284 | 620 | 856 | 1.15e-166 | 484 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97256.t1 | ATMG00160 | 88.764 | 267 | 23 | 2 | 18 | 284 | 1 | 260 | 8.16e-172 | 475 |
| MS.gene97256.t1 | AT2G07695 | 73.148 | 108 | 22 | 2 | 18 | 125 | 1 | 101 | 1.36e-43 | 150 |
Find 0 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAAATCAGATCTCTATTT+CGG | + | 37480:15891-15910 | MS.gene97256:CDS | 20.0% |
| ! | TAAGCAAAGGATAATTAGTA+GGG | - | 37480:15257-15276 | None:intergenic | 25.0% |
| !! | AATAATACGTAGATGAGTTT+TGG | - | 37480:15797-15816 | None:intergenic | 25.0% |
| !! | ATTTGCTCTTTTATACTCAA+TGG | + | 37480:15579-15598 | MS.gene97256:CDS | 25.0% |
| AATCAATTAATCCCACAAAC+AGG | + | 37480:16030-16049 | MS.gene97256:CDS | 30.0% | |
| ATCAATTAATCCCACAAACA+GGG | + | 37480:16031-16050 | MS.gene97256:CDS | 30.0% | |
| CTAAGCAAAGGATAATTAGT+AGG | - | 37480:15258-15277 | None:intergenic | 30.0% | |
| CTAGTAAAGATTATGGTTCT+CGG | + | 37480:16001-16020 | MS.gene97256:CDS | 30.0% | |
| CTTGAAATCCTAATTGCAAT+GGG | - | 37480:15336-15355 | None:intergenic | 30.0% | |
| GAGTATAAAAGAGCAAATGA+TGG | - | 37480:15577-15596 | None:intergenic | 30.0% | |
| TAGTAAAGATTATGGTTCTC+GGG | + | 37480:16002-16021 | MS.gene97256:CDS | 30.0% | |
| TCAATCACGTTTATTAGAAG+TGG | + | 37480:15750-15769 | MS.gene97256:CDS | 30.0% | |
| TCTATTATTCCTTGCATCAT+AGG | - | 37480:15370-15389 | None:intergenic | 30.0% | |
| TCTTGAAATCCTAATTGCAA+TGG | - | 37480:15337-15356 | None:intergenic | 30.0% | |
| TGTTTGTGGGATTAATTGAT+TGG | - | 37480:16031-16050 | None:intergenic | 30.0% | |
| ! | TTTCTTCCTGATTCTGATTT+TGG | + | 37480:15408-15427 | MS.gene97256:CDS | 30.0% |
| ! | TTTTGACAGTTATACGATTC+CGG | + | 37480:15708-15727 | MS.gene97256:CDS | 30.0% |
| !!! | TGATTTTGGTTTTCGTATCA+CGG | + | 37480:15422-15441 | MS.gene97256:CDS | 30.0% |
| AAAAGTGAGTGACTGTTCAT+CGG | - | 37480:15692-15711 | None:intergenic | 35.0% | |
| ATGGTATAGCAATGAACATC+GGG | - | 37480:15558-15577 | None:intergenic | 35.0% | |
| ATTAGAAGTGGACAATAGAG+TGG | + | 37480:15762-15781 | MS.gene97256:CDS | 35.0% | |
| CGAAAACCAAAATCAGAATC+AGG | - | 37480:15417-15436 | None:intergenic | 35.0% | |
| CGAGAACCATAATCTTTACT+AGG | - | 37480:16003-16022 | None:intergenic | 35.0% | |
| CTATTGGACATCAATGGTAT+CGG | + | 37480:15638-15657 | MS.gene97256:CDS | 35.0% | |
| CTATTTCGGTACAAAGAGAA+GGG | + | 37480:15905-15924 | MS.gene97256:CDS | 35.0% | |
| GAACTACTATCGAGATTCTT+CGG | + | 37480:15512-15531 | MS.gene97256:CDS | 35.0% | |
| GATGGTATAGCAATGAACAT+CGG | - | 37480:15559-15578 | None:intergenic | 35.0% | |
| GCTGTTCCTAGTAAAGATTA+TGG | + | 37480:15994-16013 | MS.gene97256:CDS | 35.0% | |
| GGGAATTGAAGAAGATCAAT+CGG | - | 37480:15289-15308 | None:intergenic | 35.0% | |
| GTTCCATGAACAATTCTTTG+CGG | - | 37480:15496-15515 | None:intergenic | 35.0% | |
| TATTTCGGTACAAAGAGAAG+GGG | + | 37480:15906-15925 | MS.gene97256:CDS | 35.0% | |
| TCAAAGCTATTGGACATCAA+TGG | + | 37480:15632-15651 | MS.gene97256:CDS | 35.0% | |
| TCAATTAATCCCACAAACAG+GGG | + | 37480:16032-16051 | MS.gene97256:CDS | 35.0% | |
| TCTATTTCGGTACAAAGAGA+AGG | + | 37480:15904-15923 | MS.gene97256:CDS | 35.0% | |
| TTCCATGAACAATTCTTTGC+GGG | - | 37480:15495-15514 | None:intergenic | 35.0% | |
| ! | TCTTTTATACTCAATGGACG+AGG | + | 37480:15585-15604 | MS.gene97256:CDS | 35.0% |
| !! | TCCAATAGCTTTGATAGTCA+TGG | - | 37480:15626-15645 | None:intergenic | 35.0% |
| AGCATCACATTTGACACCTA+AGG | - | 37480:15860-15879 | None:intergenic | 40.0% | |
| ATCCCGCAAAGAATTGTTCA+TGG | + | 37480:15490-15509 | MS.gene97256:CDS | 40.0% | |
| ATGAACATCGGGATGATACT+AGG | - | 37480:15547-15566 | None:intergenic | 40.0% | |
| CAAAGAGAAGGGGTTTACTA+TGG | + | 37480:15916-15935 | MS.gene97256:CDS | 40.0% | |
| CATCCTGCGAAGAAAATCTT+GGG | - | 37480:15224-15243 | None:intergenic | 40.0% | |
| CCCAATTCTAGATCATCTTC+CGG | - | 37480:15730-15749 | None:intergenic | 40.0% | |
| CGGGATGATACTAGGAAATA+TGG | - | 37480:15539-15558 | None:intergenic | 40.0% | |
| GAACAATTCTTTGCGGGATT+GGG | - | 37480:15489-15508 | None:intergenic | 40.0% | |
| GAAGAAGATCAATCGGAAGA+AGG | - | 37480:15282-15301 | None:intergenic | 40.0% | |
| GAGATCTGATTTAAACGACC+AGG | - | 37480:15886-15905 | None:intergenic | 40.0% | |
| GCCATGACTATCAAAGCTAT+TGG | + | 37480:15622-15641 | MS.gene97256:CDS | 40.0% | |
| TCACATTTGACACCTAAGGA+AGG | - | 37480:15856-15875 | None:intergenic | 40.0% | |
| TGAACAATTCTTTGCGGGAT+TGG | - | 37480:15490-15509 | None:intergenic | 40.0% | |
| TGCTGATGTACCTCATAGTT+GGG | + | 37480:15825-15844 | MS.gene97256:CDS | 40.0% | |
| ! | ATACCCAAGATTTTCTTCGC+AGG | + | 37480:15218-15237 | MS.gene97256:CDS | 40.0% |
| ! | CCGGAAGATGATCTAGAATT+GGG | + | 37480:15727-15746 | MS.gene97256:CDS | 40.0% |
| ! | TCCGGAAGATGATCTAGAAT+TGG | + | 37480:15726-15745 | MS.gene97256:CDS | 40.0% |
| !! | ATACGTAGATGAGTTTTGGC+TGG | - | 37480:15793-15812 | None:intergenic | 40.0% |
| !! | ATAGCTTTGATAGTCATGGC+TGG | - | 37480:15622-15641 | None:intergenic | 40.0% |
| CTGCTGATGTACCTCATAGT+TGG | + | 37480:15824-15843 | MS.gene97256:CDS | 45.0% | |
| GCATCCTGCGAAGAAAATCT+TGG | - | 37480:15225-15244 | None:intergenic | 45.0% | |
| GTCAAATGTGATGCTGTACC+TGG | + | 37480:15865-15884 | MS.gene97256:CDS | 45.0% | |
| TCGGAAGAAGGACTAAGCAA+AGG | - | 37480:15270-15289 | None:intergenic | 45.0% | |
| TTCTACGACGATAGGCGTAA+AGG | - | 37480:15974-15993 | None:intergenic | 45.0% | |
| ! | GGTTTTCGTATCACGGATCT+TGG | + | 37480:15429-15448 | MS.gene97256:CDS | 45.0% |
| AGTTGGGCTGTACCTTCCTT+AGG | + | 37480:15841-15860 | MS.gene97256:CDS | 50.0% | |
| GCAGCAACACCTATGATGCA+AGG | + | 37480:15358-15377 | MS.gene97256:CDS | 50.0% | |
| GGAACAGCTTCTACGACGAT+AGG | - | 37480:15982-16001 | None:intergenic | 50.0% | |
| GGTCAGTGCAGTGAGATTTG+TGG | + | 37480:15937-15956 | MS.gene97256:CDS | 50.0% | |
| ! | GAAGGTACAGCCCAACTATG+AGG | - | 37480:15838-15857 | None:intergenic | 50.0% |
| CTGCGCTCTTTCTGATGCAG+CGG | + | 37480:15309-15328 | MS.gene97256:CDS | 55.0% | |
| GCAGCGGACCCATTGCAATT+AGG | + | 37480:15325-15344 | MS.gene97256:CDS | 55.0% | |
| ! | CTGCATCAGAAAGAGCGCAG+GGG | - | 37480:15309-15328 | None:intergenic | 55.0% |
| ! | GCTGCATCAGAAAGAGCGCA+GGG | - | 37480:15310-15329 | None:intergenic | 55.0% |
| ! | GGATCTTGGTTCGCGCTTTG+TGG | + | 37480:15443-15462 | MS.gene97256:CDS | 55.0% |
| ! | CGCTGCATCAGAAAGAGCGC+AGG | - | 37480:15311-15330 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 37480 | gene | 15208 | 16062 | 15208 | ID=MS.gene97256 |
| 37480 | mRNA | 15208 | 16062 | 15208 | ID=MS.gene97256.t1;Parent=MS.gene97256 |
| 37480 | exon | 15208 | 16062 | 15208 | ID=MS.gene97256.t1.exon1;Parent=MS.gene97256.t1 |
| 37480 | CDS | 15208 | 16062 | 15208 | ID=cds.MS.gene97256.t1;Parent=MS.gene97256.t1 |
| Gene Sequence |
| Protein sequence |
