AlfalfaGEDB | MS.gene97345

M. sativa cultivar XinJiangDaYe / MS.gene97345

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97345.t1	XP_013470478.1	98.8	334	4	0	1	334	1	334	3.70E-181	644

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97345.t1	O80624	48.5	328	115	12	9	329	8	288	2.4e-59	230.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97345.t1	A0A072VS60	98.8	334	4	0	1	334	1	334	2.7e-181	644.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene056387	MS.gene97345	-0.80074	1.25E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97345.t1	MTR_1g115605	98.802	334	4	0	1	334	1	334	0.0	686
MS.gene97345.t1	MTR_1g067410	43.030	330	135	13	13	331	16	303	4.68e-45	157

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97345.t1	AT2G39370	48.485	330	112	13	6	326	5	285	1.28e-61	200
MS.gene97345.t1	AT2G37380	35.777	341	149	10	3	329	8	292	1.40e-22	96.7

Find 56 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCTCTTGAAGCCTTTAATT+TGG	0.146793	1.2:+85816818	None:intergenic
GTTGGAATCAAACTTCTTTC+AGG	0.146827	1.2:-85816549	MS.gene97345:CDS
GATAGCTTTGCCTTTGAATT+TGG	0.163953	1.2:+85816652	None:intergenic
TTCTTTAGGTTCCAAATTAA+AGG	0.205767	1.2:-85816829	MS.gene97345:CDS
AAACTGAAACAGTCTTCTTT+AGG	0.271143	1.2:-85816843	MS.gene97345:CDS
TCTTAGTCCATGACTTCTTC+TGG	0.278627	1.2:+85816865	None:intergenic
CTTGAACAATGGAAGACATT+TGG	0.293756	1.2:+85817213	None:intergenic
TGCCTTTGAATTTGGCCATA+TGG	0.329386	1.2:+85816660	None:intergenic
AAGTCCTGGTTTGGAAAATC+TGG	0.332844	1.2:-85816789	MS.gene97345:CDS
TCACTTTCAAACAAATCATA+TGG	0.358419	1.2:-85816480	MS.gene97345:CDS
TCATCAAATTCCTCGATATC+TGG	0.379426	1.2:-85816516	MS.gene97345:CDS
ATCAGATGGTGAAATATTGC+AGG	0.390323	1.2:+85816977	None:intergenic
GCATATCTCAAGTCCTGGTT+TGG	0.399275	1.2:-85816798	MS.gene97345:CDS
TGCAGTGTGCAATTGCTCCT+TGG	0.412534	1.2:+85816391	None:intergenic
TCAACAAAAGTTGCTGATGA+AGG	0.445650	1.2:-85816741	MS.gene97345:CDS
TCATCAGCTGGTGAAGTTGT+TGG	0.457440	1.2:+85817182	None:intergenic
AAAGAAAAGAGCAGTGAAGA+TGG	0.463735	1.2:-85816600	MS.gene97345:CDS
AAGGCAAAGCTATCAGTCTT+CGG	0.466088	1.2:-85816643	MS.gene97345:CDS
GTAAAAGTAGTAGTGGCTAA+TGG	0.470831	1.2:+85817029	None:intergenic
CCTTCCACCTCGGTTACAAA+TGG	0.472089	1.2:-85817126	MS.gene97345:CDS
TCAAATGGTGTGCCGGTGAT+CGG	0.478034	1.2:+85817002	None:intergenic
CACAGAAGATCATTCTCTGT+TGG	0.492725	1.2:-85816567	MS.gene97345:CDS
AAACTCACCAGCTTCTCAAA+AGG	0.497441	1.2:-85816449	MS.gene97345:CDS
AAGGTTCAGTTTCAAAAGCA+AGG	0.506978	1.2:-85816722	MS.gene97345:CDS
TGGCTCTTTCTCTTGAACAA+TGG	0.508604	1.2:+85817202	None:intergenic
ATCCATATGGCCAAATTCAA+AGG	0.520385	1.2:-85816662	MS.gene97345:CDS
CAACCTCAAAAGGTTTGTGA+GGG	0.520895	1.2:+85817084	None:intergenic
TAACCGAGGTGGAAGGTGAA+GGG	0.527822	1.2:+85817133	None:intergenic
GTAACCGAGGTGGAAGGTGA+AGG	0.531115	1.2:+85817132	None:intergenic
TCAAACTCTCTAGGATGTTG+TGG	0.534718	1.2:+85817239	None:intergenic
CAAGAGCATATCTCAAGTCC+TGG	0.537010	1.2:-85816803	MS.gene97345:CDS
GAAATATTGCAGGACTCAAA+TGG	0.540473	1.2:+85816987	None:intergenic
ATTTGGAACTCAAACTCTCT+AGG	0.552249	1.2:+85817230	None:intergenic
TCAACCTCAAAAGGTTTGTG+AGG	0.554753	1.2:+85817083	None:intergenic
TTGAAAACCAGAAGAAGTCA+TGG	0.561084	1.2:-85816872	MS.gene97345:CDS
GAGATAGAAAACTCAATCCA+AGG	0.565645	1.2:-85816408	MS.gene97345:CDS
TTGTAGAAAAGCTCATCAGC+TGG	0.575859	1.2:+85817170	None:intergenic
CGGCGTTGTAAAAGTAGTAG+TGG	0.581269	1.2:+85817022	None:intergenic
AGGTTCAGTTTCAAAAGCAA+GGG	0.584246	1.2:-85816721	MS.gene97345:CDS
TCGATGTAATCATCATCTGA+AGG	0.584817	1.2:+85817317	None:intergenic
GTCGAGGTTATAATACTCCT+CGG	0.586101	1.2:+85816926	None:intergenic
AACCGAGGTGGAAGGTGAAG+GGG	0.586622	1.2:+85817134	None:intergenic
CGATGTAATCATCATCTGAA+GGG	0.601976	1.2:+85817318	None:intergenic
ACCGAGGTGGAAGGTGAAGG+GGG	0.612840	1.2:+85817135	None:intergenic
TATCCGTTGTGTGGTAGTCG+AGG	0.615070	1.2:+85816910	None:intergenic
GCAGGACTCAAATGGTGTGC+CGG	0.615680	1.2:+85816995	None:intergenic
TCTTCGGTTTCTGTCATGAG+AGG	0.620110	1.2:-85816627	MS.gene97345:CDS
TACCACACAACGGATACAAG+TGG	0.625294	1.2:-85816903	MS.gene97345:CDS
ATCCACTTGTATCCGTTGTG+TGG	0.626565	1.2:+85816901	None:intergenic
CTAACTTGACAAGAATCAGA+TGG	0.628578	1.2:+85816963	None:intergenic
TGACGATGAACCAGATATCG+AGG	0.629004	1.2:+85816506	None:intergenic
AGATGATGATTACATCGACA+TGG	0.635328	1.2:-85817312	MS.gene97345:CDS
CCATTTGTAACCGAGGTGGA+AGG	0.639907	1.2:+85817126	None:intergenic
TAGTCGAGAACTAAATGCCG+AGG	0.663489	1.2:-85816943	MS.gene97345:CDS
TCGACCATTTGTAACCGAGG+TGG	0.669165	1.2:+85817122	None:intergenic
TAACCTCGACTACCACACAA+CGG	0.724478	1.2:-85816913	MS.gene97345:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTAAATAAGCATGTTCA+AGG	-	chr1.2:85817016-85817035	MS.gene97345:CDS	20.0%
!	TCACTTTCAAACAAATCATA+TGG	-	chr1.2:85817232-85817251	MS.gene97345:CDS	25.0%
!!	TTCTTTAGGTTCCAAATTAA+AGG	-	chr1.2:85816883-85816902	MS.gene97345:CDS	25.0%
	AAACTGAAACAGTCTTCTTT+AGG	-	chr1.2:85816869-85816888	MS.gene97345:CDS	30.0%
	GGAGCAAATAAAAATCCATA+TGG	-	chr1.2:85817037-85817056	MS.gene97345:CDS	30.0%
!	ATATCCTTTTCAACCTCAAA+AGG	+	chr1.2:85816641-85816660	None:intergenic	30.0%
	AAAGAAAAGAGCAGTGAAGA+TGG	-	chr1.2:85817112-85817131	MS.gene97345:CDS	35.0%
	AAGGTTCAGTTTCAAAAGCA+AGG	-	chr1.2:85816990-85817009	MS.gene97345:CDS	35.0%
	AGATGATGATTACATCGACA+TGG	-	chr1.2:85816400-85816419	MS.gene97345:CDS	35.0%
	AGGTTCAGTTTCAAAAGCAA+GGG	-	chr1.2:85816991-85817010	MS.gene97345:CDS	35.0%
	ATCAGATGGTGAAATATTGC+AGG	+	chr1.2:85816738-85816757	None:intergenic	35.0%
	ATCCATATGGCCAAATTCAA+AGG	-	chr1.2:85817050-85817069	MS.gene97345:CDS	35.0%
	CGATGTAATCATCATCTGAA+GGG	+	chr1.2:85816397-85816416	None:intergenic	35.0%
	CTAACTTGACAAGAATCAGA+TGG	+	chr1.2:85816752-85816771	None:intergenic	35.0%
	CTTGAACAATGGAAGACATT+TGG	+	chr1.2:85816502-85816521	None:intergenic	35.0%
	GAAATATTGCAGGACTCAAA+TGG	+	chr1.2:85816728-85816747	None:intergenic	35.0%
	GAGATAGAAAACTCAATCCA+AGG	-	chr1.2:85817304-85817323	MS.gene97345:CDS	35.0%
	GTAAAAGTAGTAGTGGCTAA+TGG	+	chr1.2:85816686-85816705	None:intergenic	35.0%
	GTTGGAATCAAACTTCTTTC+AGG	-	chr1.2:85817163-85817182	MS.gene97345:CDS	35.0%
	TCAACAAAAGTTGCTGATGA+AGG	-	chr1.2:85816971-85816990	MS.gene97345:CDS	35.0%
	TCATCAAATTCCTCGATATC+TGG	-	chr1.2:85817196-85817215	MS.gene97345:CDS	35.0%
	TCGATGTAATCATCATCTGA+AGG	+	chr1.2:85816398-85816417	None:intergenic	35.0%
	TGCTCTTGAAGCCTTTAATT+TGG	+	chr1.2:85816897-85816916	None:intergenic	35.0%
	TTGAAAACCAGAAGAAGTCA+TGG	-	chr1.2:85816840-85816859	MS.gene97345:CDS	35.0%
!	ATTTGGAACTCAAACTCTCT+AGG	+	chr1.2:85816485-85816504	None:intergenic	35.0%
!	GATAGCTTTGCCTTTGAATT+TGG	+	chr1.2:85817063-85817082	None:intergenic	35.0%
!!!	CAAACCTTTTGAGGTTGAAA+AGG	-	chr1.2:85816634-85816653	MS.gene97345:CDS	35.0%
	AAACTCACCAGCTTCTCAAA+AGG	-	chr1.2:85817263-85817282	MS.gene97345:CDS	40.0%
	AAGGCAAAGCTATCAGTCTT+CGG	-	chr1.2:85817069-85817088	MS.gene97345:CDS	40.0%
	CAACCTCAAAAGGTTTGTGA+GGG	+	chr1.2:85816631-85816650	None:intergenic	40.0%
	CACAGAAGATCATTCTCTGT+TGG	-	chr1.2:85817145-85817164	MS.gene97345:CDS	40.0%
	GTCGAGGTTATAATACTCCT+CGG	+	chr1.2:85816789-85816808	None:intergenic	40.0%
	TCAACCTCAAAAGGTTTGTG+AGG	+	chr1.2:85816632-85816651	None:intergenic	40.0%
	TCTTAGTCCATGACTTCTTC+TGG	+	chr1.2:85816850-85816869	None:intergenic	40.0%
	TGCCTTTGAATTTGGCCATA+TGG	+	chr1.2:85817055-85817074	None:intergenic	40.0%
	TGGCTCTTTCTCTTGAACAA+TGG	+	chr1.2:85816513-85816532	None:intergenic	40.0%
	TTGTAGAAAAGCTCATCAGC+TGG	+	chr1.2:85816545-85816564	None:intergenic	40.0%
!	AAACCCTCACAAACCTTTTG+AGG	-	chr1.2:85816625-85816644	MS.gene97345:CDS	40.0%
!	ACAACCAGATTTTCCAAACC+AGG	+	chr1.2:85816930-85816949	None:intergenic	40.0%
!	GCTGATGAGCTTTTCTACAA+AGG	-	chr1.2:85816545-85816564	MS.gene97345:CDS	40.0%
!	TTTTCGACCATTTGTAACCG+AGG	+	chr1.2:85816596-85816615	None:intergenic	40.0%
!!	AAGGTTTGTGAGGGTTTTGA+AGG	+	chr1.2:85816622-85816641	None:intergenic	40.0%
!!	AAGTCCTGGTTTGGAAAATC+TGG	-	chr1.2:85816923-85816942	MS.gene97345:CDS	40.0%
!!	TCAAACTCTCTAGGATGTTG+TGG	+	chr1.2:85816476-85816495	None:intergenic	40.0%
	ATCCACTTGTATCCGTTGTG+TGG	+	chr1.2:85816814-85816833	None:intergenic	45.0%
	CGGCGTTGTAAAAGTAGTAG+TGG	+	chr1.2:85816693-85816712	None:intergenic	45.0%
	GCATATCTCAAGTCCTGGTT+TGG	-	chr1.2:85816914-85816933	MS.gene97345:CDS	45.0%
	TAACCTCGACTACCACACAA+CGG	-	chr1.2:85816799-85816818	MS.gene97345:CDS	45.0%
	TACCACACAACGGATACAAG+TGG	-	chr1.2:85816809-85816828	MS.gene97345:CDS	45.0%
	TAGTCGAGAACTAAATGCCG+AGG	-	chr1.2:85816769-85816788	MS.gene97345:CDS	45.0%
	TCATCAGCTGGTGAAGTTGT+TGG	+	chr1.2:85816533-85816552	None:intergenic	45.0%
	TCTTCGGTTTCTGTCATGAG+AGG	-	chr1.2:85817085-85817104	MS.gene97345:CDS	45.0%
	TGACGATGAACCAGATATCG+AGG	+	chr1.2:85817209-85817228	None:intergenic	45.0%
!	ACTTTTACAACGCCGATCAC+CGG	-	chr1.2:85816698-85816717	MS.gene97345:CDS	45.0%
!	CAAGAGCATATCTCAAGTCC+TGG	-	chr1.2:85816909-85816928	MS.gene97345:CDS	45.0%
!!	TGTTGCACCTTTTGAGAAGC+TGG	+	chr1.2:85817273-85817292	None:intergenic	45.0%
	CCATTTGTAACCGAGGTGGA+AGG	+	chr1.2:85816589-85816608	None:intergenic	50.0%
	CCTTCCACCTCGGTTACAAA+TGG	-	chr1.2:85816586-85816605	MS.gene97345:CDS	50.0%
	TAACCGAGGTGGAAGGTGAA+GGG	+	chr1.2:85816582-85816601	None:intergenic	50.0%
	TATCCGTTGTGTGGTAGTCG+AGG	+	chr1.2:85816805-85816824	None:intergenic	50.0%
	TCGACCATTTGTAACCGAGG+TGG	+	chr1.2:85816593-85816612	None:intergenic	50.0%
	TGCAGTGTGCAATTGCTCCT+TGG	+	chr1.2:85817324-85817343	None:intergenic	50.0%
!	TCAAATGGTGTGCCGGTGAT+CGG	+	chr1.2:85816713-85816732	None:intergenic	50.0%
	AACCGAGGTGGAAGGTGAAG+GGG	+	chr1.2:85816581-85816600	None:intergenic	55.0%
	GCAGGACTCAAATGGTGTGC+CGG	+	chr1.2:85816720-85816739	None:intergenic	55.0%
	GTAACCGAGGTGGAAGGTGA+AGG	+	chr1.2:85816583-85816602	None:intergenic	55.0%
	ACCGAGGTGGAAGGTGAAGG+GGG	+	chr1.2:85816580-85816599	None:intergenic	60.0%
	TCCCCCTTCACCTTCCACCT+CGG	-	chr1.2:85816576-85816595	MS.gene97345:CDS	60.0%
	GAGGTGGAAGGTGAAGGGGG+AGG	+	chr1.2:85816577-85816596	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	85816365	85817369	85816365	ID=MS.gene97345
chr1.2	mRNA	85816365	85817369	85816365	ID=MS.gene97345.t1;Parent=MS.gene97345
chr1.2	exon	85816365	85817369	85816365	ID=MS.gene97345.t1.exon1;Parent=MS.gene97345.t1
chr1.2	CDS	85816365	85817369	85816365	ID=cds.MS.gene97345.t1;Parent=MS.gene97345.t1

Gene Sequence

>MS.gene97345

ATGACTTCAAAAACACTCTTAGCATGTGACCCTTCAGATGATGATTACATCGACATGGAAGTTAGTTCATACTCAAAATTCTTCAATCATCATTCTGAAAACTCACATCCACAACATCCTAGAGAGTTTGAGTTCCAAATGTCTTCCATTGTTCAAGAGAAAGAGCCAACAACTTCACCAGCTGATGAGCTTTTCTACAAAGGAAAACTCCTCCCCCTTCACCTTCCACCTCGGTTACAAATGGTCGAAAAACTCCTTCAAAACCCTCACAAACCTTTTGAGGTTGAAAAGGATATCTTTGAAGAGTTCTATAGCACTCCATTAGCCACTACTACTTTTACAACGCCGATCACCGGCACACCATTTGAGTCCTGCAATATTTCACCATCTGATTCTTGTCAAGTTAGTCGAGAACTAAATGCCGAGGAGTATTATAACCTCGACTACCACACAACGGATACAAGTGGATTCGTTGTTGAAAACCAGAAGAAGTCATGGACTAAGAAACTGAAACAGTCTTCTTTAGGTTCCAAATTAAAGGCTTCAAGAGCATATCTCAAGTCCTGGTTTGGAAAATCTGGTTGTTCTTATGAAAACTATGCTTCATCAACAAAAGTTGCTGATGAAGGTTCAGTTTCAAAAGCAAGGGAAATTTTAAATAAGCATGTTCAAGGAGCAAATAAAAATCCATATGGCCAAATTCAAAGGCAAAGCTATCAGTCTTCGGTTTCTGTCATGAGAGGATACAAAGAAAAGAGCAGTGAAGATGGAAGTAATCATCACAGAAGATCATTCTCTGTTGGAATCAAACTTCTTTCAGGAAACAAGTCATCATCAAATTCCTCGATATCTGGTTCATCGTCATTTTCACTTTCAAACAAATCATATGGATGTCATCAAACTCACCAGCTTCTCAAAAGGTGCAACAGTGCAAGTTCAGAGATAGAAAACTCAATCCAAGGAGCAATTGCACACTGCAAGAAGTCTCAGCAAATTTTAACTTGA

Protein sequence

>MS.gene97345.t1

MTSKTLLACDPSDDDYIDMEVSSYSKFFNHHSENSHPQHPREFEFQMSSIVQEKEPTTSPADELFYKGKLLPLHLPPRLQMVEKLLQNPHKPFEVEKDIFEEFYSTPLATTTFTTPITGTPFESCNISPSDSCQVSRELNAEEYYNLDYHTTDTSGFVVENQKKSWTKKLKQSSLGSKLKASRAYLKSWFGKSGCSYENYASSTKVADEGSVSKAREILNKHVQGANKNPYGQIQRQSYQSSVSVMRGYKEKSSEDGSNHHRRSFSVGIKLLSGNKSSSNSSISGSSSFSLSNKSYGCHQTHQLLKRCNSASSEIENSIQGAIAHCKKSQQILT