Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97400.t1 | AGC78837.1 | 92.2 | 258 | 20 | 0 | 15 | 272 | 1 | 258 | 1.60E-129 | 472.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97400.t1 | P05491 | 96.3 | 245 | 6 | 1 | 23 | 267 | 12 | 253 | 1.5e-131 | 470.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97400.t1 | R4IUC9 | 92.2 | 258 | 20 | 0 | 15 | 272 | 1 | 258 | 1.2e-129 | 472.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050945 | MS.gene97400 | -0.808647 | 2.79E-50 | -1.69E-46 |
| MS.gene051043 | MS.gene97400 | -0.810374 | 1.19E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97400.t1 | MTR_0212s0040 | 93.145 | 248 | 5 | 1 | 25 | 272 | 620 | 855 | 9.82e-163 | 473 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97400.t1 | ATMG00160 | 87.218 | 266 | 27 | 2 | 7 | 272 | 1 | 259 | 1.18e-167 | 464 |
| MS.gene97400.t1 | AT2G07695 | 73.148 | 108 | 22 | 2 | 7 | 114 | 1 | 101 | 1.33e-43 | 150 |
Find 0 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAAATCAGATCTCTATTT+CGG | - | 57107:4929-4948 | MS.gene97400:CDS | 20.0% |
| ! | AATCAATTAATCCACAAACA+GGG | - | 57107:5068-5087 | MS.gene97400:CDS | 25.0% |
| ! | TAAGCAAAGGATAATTAGTA+GGG | + | 57107:4295-4314 | None:intergenic | 25.0% |
| !! | AATAATACGTAGATGAGTTT+TGG | + | 57107:4835-4854 | None:intergenic | 25.0% |
| !! | ATTTGCTCTTTTATACTCAA+TGG | - | 57107:4617-4636 | MS.gene97400:CDS | 25.0% |
| ATCAATTAATCCACAAACAG+GGG | - | 57107:5069-5088 | MS.gene97400:CDS | 30.0% | |
| CAATCAATTAATCCACAAAC+AGG | - | 57107:5067-5086 | MS.gene97400:CDS | 30.0% | |
| CTAAGCAAAGGATAATTAGT+AGG | + | 57107:4296-4315 | None:intergenic | 30.0% | |
| CTAGTAAAGATTATGGTTCT+CGG | - | 57107:5039-5058 | MS.gene97400:CDS | 30.0% | |
| CTTGAAATCCTAATTGCAAT+GGG | + | 57107:4374-4393 | None:intergenic | 30.0% | |
| GAGTATAAAAGAGCAAATGA+TGG | + | 57107:4615-4634 | None:intergenic | 30.0% | |
| TAGTAAAGATTATGGTTCTC+GGG | - | 57107:5040-5059 | MS.gene97400:CDS | 30.0% | |
| TCAATCACGTTTATTAGAAG+TGG | - | 57107:4788-4807 | MS.gene97400:CDS | 30.0% | |
| TCTATTATTCCTTGCATCAT+AGG | + | 57107:4408-4427 | None:intergenic | 30.0% | |
| TCTTGAAATCCTAATTGCAA+TGG | + | 57107:4375-4394 | None:intergenic | 30.0% | |
| ! | CTGTTTGTGGATTAATTGAT+TGG | + | 57107:5069-5088 | None:intergenic | 30.0% |
| ! | TTTCTTCCTGATTCTGATTT+TGG | - | 57107:4446-4465 | MS.gene97400:CDS | 30.0% |
| ! | TTTTGACAGTTATACGATTC+CGG | - | 57107:4746-4765 | MS.gene97400:CDS | 30.0% |
| !!! | TGATTTTGGTTTTCGTATCA+CGG | - | 57107:4460-4479 | MS.gene97400:CDS | 30.0% |
| AAAAGTGAGTGACTGTTCAT+CGG | + | 57107:4730-4749 | None:intergenic | 35.0% | |
| ATGGTATAGCAATGAACATC+GGG | + | 57107:4596-4615 | None:intergenic | 35.0% | |
| ATTAGAAGTGGACAATAGAG+TGG | - | 57107:4800-4819 | MS.gene97400:CDS | 35.0% | |
| CGAAAACCAAAATCAGAATC+AGG | + | 57107:4455-4474 | None:intergenic | 35.0% | |
| CGAGAACCATAATCTTTACT+AGG | + | 57107:5041-5060 | None:intergenic | 35.0% | |
| CTATTGGACATCAATGGTAT+CGG | - | 57107:4676-4695 | MS.gene97400:CDS | 35.0% | |
| CTATTTCGGTACAAAGAGAA+GGG | - | 57107:4943-4962 | MS.gene97400:CDS | 35.0% | |
| GAACTACTATCGAGATTCTT+CGG | - | 57107:4550-4569 | MS.gene97400:CDS | 35.0% | |
| GATGGTATAGCAATGAACAT+CGG | + | 57107:4597-4616 | None:intergenic | 35.0% | |
| GCTGTTCCTAGTAAAGATTA+TGG | - | 57107:5032-5051 | MS.gene97400:CDS | 35.0% | |
| GGGAATTGAAGAAGATCAAT+CGG | + | 57107:4327-4346 | None:intergenic | 35.0% | |
| GTTCCATGAACAATTCTTTG+CGG | + | 57107:4534-4553 | None:intergenic | 35.0% | |
| TATTTCGGTACAAAGAGAAG+GGG | - | 57107:4944-4963 | MS.gene97400:CDS | 35.0% | |
| TCAAAGCTATTGGACATCAA+TGG | - | 57107:4670-4689 | MS.gene97400:CDS | 35.0% | |
| TCTATTTCGGTACAAAGAGA+AGG | - | 57107:4942-4961 | MS.gene97400:CDS | 35.0% | |
| TTCCATGAACAATTCTTTGC+GGG | + | 57107:4533-4552 | None:intergenic | 35.0% | |
| ! | TCTTTTATACTCAATGGACG+AGG | - | 57107:4623-4642 | MS.gene97400:CDS | 35.0% |
| !! | TCCAATAGCTTTGATAGTCA+TGG | + | 57107:4664-4683 | None:intergenic | 35.0% |
| AGCATCACATTTGACACCTA+AGG | + | 57107:4898-4917 | None:intergenic | 40.0% | |
| ATCCCGCAAAGAATTGTTCA+TGG | - | 57107:4528-4547 | MS.gene97400:CDS | 40.0% | |
| ATGAACATCGGGATGATACT+AGG | + | 57107:4585-4604 | None:intergenic | 40.0% | |
| CAAAGAGAAGGGGTTTACTA+TGG | - | 57107:4954-4973 | MS.gene97400:CDS | 40.0% | |
| CCCAATTCTAGATCATCTTC+CGG | + | 57107:4768-4787 | None:intergenic | 40.0% | |
| CGGGATGATACTAGGAAATA+TGG | + | 57107:4577-4596 | None:intergenic | 40.0% | |
| GAACAATTCTTTGCGGGATT+GGG | + | 57107:4527-4546 | None:intergenic | 40.0% | |
| GAAGAAGATCAATCGGAAGA+AGG | + | 57107:4320-4339 | None:intergenic | 40.0% | |
| GAGATCTGATTTAAACGACC+AGG | + | 57107:4924-4943 | None:intergenic | 40.0% | |
| GCCATGACTATCAAAGCTAT+TGG | - | 57107:4660-4679 | MS.gene97400:CDS | 40.0% | |
| TCACATTTGACACCTAAGGA+AGG | + | 57107:4894-4913 | None:intergenic | 40.0% | |
| TGAACAATTCTTTGCGGGAT+TGG | + | 57107:4528-4547 | None:intergenic | 40.0% | |
| TGCTGATGTACCTCATAGTT+GGG | - | 57107:4863-4882 | MS.gene97400:CDS | 40.0% | |
| ! | CCGGAAGATGATCTAGAATT+GGG | - | 57107:4765-4784 | MS.gene97400:CDS | 40.0% |
| ! | TCCGGAAGATGATCTAGAAT+TGG | - | 57107:4764-4783 | MS.gene97400:CDS | 40.0% |
| !! | ATACGTAGATGAGTTTTGGC+TGG | + | 57107:4831-4850 | None:intergenic | 40.0% |
| !! | ATAGCTTTGATAGTCATGGC+TGG | + | 57107:4660-4679 | None:intergenic | 40.0% |
| ACAAACAGGGGAAGCTTAAG+CGG | - | 57107:5081-5100 | MS.gene97400:CDS | 45.0% | |
| CTGCTGATGTACCTCATAGT+TGG | - | 57107:4862-4881 | MS.gene97400:CDS | 45.0% | |
| GTCAAATGTGATGCTGTACC+TGG | - | 57107:4903-4922 | MS.gene97400:CDS | 45.0% | |
| TCGGAAGAAGGACTAAGCAA+AGG | + | 57107:4308-4327 | None:intergenic | 45.0% | |
| TTCTACGACGATAGGCGTAA+AGG | + | 57107:5012-5031 | None:intergenic | 45.0% | |
| ! | GGTTTTCGTATCACGGATCT+TGG | - | 57107:4467-4486 | MS.gene97400:CDS | 45.0% |
| AGTTGGGCTGTACCTTCCTT+AGG | - | 57107:4879-4898 | MS.gene97400:CDS | 50.0% | |
| GCAGCAACACCTATGATGCA+AGG | - | 57107:4396-4415 | MS.gene97400:CDS | 50.0% | |
| GCTTAAGCTTCCCCTGTTTG+TGG | + | 57107:5082-5101 | None:intergenic | 50.0% | |
| GGAACAGCTTCTACGACGAT+AGG | + | 57107:5020-5039 | None:intergenic | 50.0% | |
| GGTCAGTGCAGTGAGATTTG+TGG | - | 57107:4975-4994 | MS.gene97400:CDS | 50.0% | |
| ! | GAAGGTACAGCCCAACTATG+AGG | + | 57107:4876-4895 | None:intergenic | 50.0% |
| CTGCGCTCTTTCTGATGCAG+CGG | - | 57107:4347-4366 | MS.gene97400:CDS | 55.0% | |
| GCAGCGGACCCATTGCAATT+AGG | - | 57107:4363-4382 | MS.gene97400:CDS | 55.0% | |
| ! | CTGCATCAGAAAGAGCGCAG+GGG | + | 57107:4347-4366 | None:intergenic | 55.0% |
| ! | GCTGCATCAGAAAGAGCGCA+GGG | + | 57107:4348-4367 | None:intergenic | 55.0% |
| ! | GGATCTTGGTTCGCGCTTTG+TGG | - | 57107:4481-4500 | MS.gene97400:CDS | 55.0% |
| ! | CGCTGCATCAGAAAGAGCGC+AGG | + | 57107:4349-4368 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57107 | gene | 4279 | 5109 | 4279 | ID=MS.gene97400 |
| 57107 | mRNA | 4279 | 5109 | 4279 | ID=MS.gene97400.t1;Parent=MS.gene97400 |
| 57107 | exon | 4279 | 5109 | 4279 | ID=MS.gene97400.t1.exon1;Parent=MS.gene97400.t1 |
| 57107 | CDS | 4279 | 5109 | 4279 | ID=cds.MS.gene97400.t1;Parent=MS.gene97400.t1 |
| Gene Sequence |
| Protein sequence |
